##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211394_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8772532 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.271069515619892 31.0 31.0 34.0 28.0 34.0 2 31.308896336884267 31.0 31.0 34.0 28.0 34.0 3 31.366238504459147 31.0 31.0 34.0 28.0 34.0 4 35.00261817226771 37.0 35.0 37.0 32.0 37.0 5 35.0564664796891 37.0 35.0 37.0 32.0 37.0 6 35.121387075020074 37.0 35.0 37.0 32.0 37.0 7 35.04925305487629 37.0 35.0 37.0 32.0 37.0 8 35.050103550491464 37.0 35.0 37.0 32.0 37.0 9 36.602648129411214 39.0 37.0 39.0 32.0 39.0 10-11 36.524273379681034 39.0 37.0 39.0 32.0 39.0 12-13 36.41765923452887 39.0 37.0 39.0 32.0 39.0 14-15 37.58357416080101 40.0 37.0 41.0 32.0 41.0 16-17 37.562638642982435 40.0 37.0 41.0 32.0 41.0 18-19 37.55984110402789 40.0 37.0 41.0 32.0 41.0 20-21 37.54287707357466 40.0 37.0 41.0 32.0 41.0 22-23 37.423853740288436 40.0 37.0 41.0 31.0 41.0 24-25 37.32825431699764 40.0 36.5 41.0 31.0 41.0 26-27 37.18927044096277 40.0 36.0 41.0 31.0 41.0 28-29 37.03968888343753 39.5 36.0 41.0 30.0 41.0 30-31 36.87485927666037 39.0 36.0 41.0 30.0 41.0 32-33 36.652424408369214 39.0 35.0 41.0 30.0 41.0 34-35 36.53656834765607 39.0 35.0 41.0 30.0 41.0 36-37 36.411719045310974 39.0 35.0 41.0 29.5 41.0 38-39 36.254745152254785 39.0 35.0 40.0 29.0 41.0 40-41 36.083386073712816 39.0 35.0 40.0 28.5 41.0 42-43 35.89740556090305 38.0 35.0 40.0 27.5 41.0 44-45 35.762898841520325 38.0 35.0 40.0 27.0 41.0 46-47 35.52740634060953 38.0 34.0 40.0 26.0 41.0 48-49 35.27514456487591 38.0 34.0 40.0 25.5 41.0 50-51 34.595766934791456 37.5 33.5 39.5 24.5 40.5 52-53 34.89663149704099 38.0 33.5 39.5 25.5 40.5 54-55 35.30378874651013 38.0 34.0 40.0 26.0 41.0 56-57 35.21829718033516 38.0 34.0 40.0 26.0 41.0 58-59 35.01344007636564 38.0 34.0 40.0 26.0 41.0 60-61 34.77771571537157 37.5 34.0 40.0 26.0 41.0 62-63 34.47557506772275 37.0 33.0 40.0 25.0 41.0 64-65 34.261691550398446 36.0 33.0 40.0 25.0 41.0 66-67 34.060201604280266 36.0 33.0 39.0 24.5 41.0 68-69 33.7195415759099 35.5 33.0 39.0 24.0 41.0 70-71 33.35311504135864 35.0 32.0 38.5 23.5 40.5 72-73 32.97405378515576 35.0 32.0 37.5 23.0 40.0 74-75 32.60500178283762 35.0 32.0 37.0 22.0 39.5 76-77 32.230313665427495 35.0 31.0 36.5 21.0 39.0 78-79 31.849061935596247 35.0 31.0 36.0 20.0 39.0 80-81 31.47894347948802 35.0 31.0 35.5 20.0 37.5 82-83 31.12200274675544 34.5 30.0 35.0 18.5 37.0 84-85 30.794122951047655 34.0 30.0 35.0 18.0 36.5 86-87 30.461297148873324 34.0 29.5 35.0 17.5 36.0 88-89 30.158551943726167 34.0 29.0 35.0 15.5 36.0 90-91 29.860629690493006 34.0 29.0 35.0 13.5 35.5 92-93 29.563604726662724 34.0 29.0 35.0 10.5 35.0 94-95 29.274208518133648 34.0 27.5 35.0 10.0 35.0 96-97 28.963914466199725 34.0 27.0 35.0 10.0 35.0 98-99 28.635525752428148 34.0 25.5 35.0 10.0 35.0 100 27.3690040686087 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3667146394773333 1101 2 -0.33794146228407485 1101 3 -0.3867395820398123 1101 4 -0.26912382519999056 1101 5 -0.34712643077370586 1101 6 -0.289801103491925 1101 7 -0.3051552504906283 1101 8 -0.37477224284423727 1101 9 -0.46696766899895437 1101 10-11 -0.3861387483936696 1101 12-13 -0.3657161844426966 1101 14-15 -0.6404691499907287 1101 16-17 -0.5811482484978328 1101 18-19 -0.5933054350611826 1101 20-21 -0.6370026586520083 1101 22-23 -0.6047657804233211 1101 24-25 -0.6490591149688925 1101 26-27 -0.7149693715108185 1101 28-29 -0.7096951620930341 1101 30-31 -0.8015583288795725 1101 32-33 -0.7325938891719233 1101 34-35 -0.74291889747591 1101 36-37 -0.742738894141894 1101 38-39 -0.8795873768083382 1101 40-41 -0.9519988815693736 1101 42-43 -0.9334115817081639 1101 44-45 -0.9039393458970011 1101 46-47 -0.8690013972685691 1101 48-49 -0.9387688172382411 1101 50-51 -0.8515115552064856 1101 52-53 -0.6231741475235495 1101 54-55 -0.6215293917758302 1101 56-57 -0.7351279689541741 1101 58-59 -0.7135630806195508 1101 60-61 -0.6612424182833934 1101 62-63 -0.8477763718722358 1101 64-65 -0.7945577032147639 1101 66-67 -0.8083414295032654 1101 68-69 -0.8918192109646625 1101 70-71 -0.7993521346005679 1101 72-73 -0.8563612018535096 1101 74-75 -0.9116680520275224 1101 76-77 -0.84782804498278 1101 78-79 -0.9364039327751144 1101 80-81 -1.0191933930693864 1101 82-83 -0.9776039802980598 1101 84-85 -1.043646473869238 1101 86-87 -1.144458631299969 1101 88-89 -1.208760169575168 1101 90-91 -1.2372883763920406 1101 92-93 -1.3467299516423878 1101 94-95 -1.3917468328599512 1101 96-97 -1.4615830845562279 1101 98-99 -1.5820496194885827 1101 100 -1.6157975944542535 1102 1 -0.35549570045989753 1102 2 -0.34819936964746034 1102 3 -0.37911513834302824 1102 4 -0.29665626524082 1102 5 -0.2787010855248653 1102 6 -0.2987783346652293 1102 7 -0.4181220209514862 1102 8 -0.30715252915588565 1102 9 -0.4735028470259337 1102 10-11 -0.4450353838550356 1102 12-13 -0.5307662584693489 1102 14-15 -0.6405600080412484 1102 16-17 -0.6215683156418166 1102 18-19 -0.6970458675646753 1102 20-21 -0.7606587200460382 1102 22-23 -0.7540829152984898 1102 24-25 -0.7141224320063202 1102 26-27 -0.7392974080859886 1102 28-29 -0.7883304128607094 1102 30-31 -0.7036151048246424 1102 32-33 -0.7884705593024108 1102 34-35 -0.8348907210081151 1102 36-37 -0.8826390763499532 1102 38-39 -0.9272372289268347 1102 40-41 -1.12834428338153 1102 42-43 -1.0584147604286542 1102 44-45 -1.0868838890078791 1102 46-47 -1.2191654391410722 1102 48-49 -1.257908700871205 1102 50-51 -1.022851046497621 1102 52-53 -0.8703705793550895 1102 54-55 -0.8866760356022425 1102 56-57 -0.8958696694370616 1102 58-59 -0.8104269685497556 1102 60-61 -0.892348510643501 1102 62-63 -0.854844218446182 1102 64-65 -0.9791021691464934 1102 66-67 -0.9419888559912621 1102 68-69 -0.8642196153666433 1102 70-71 -0.8261667535388071 1102 72-73 -0.8758676394362297 1102 74-75 -0.9899925930410802 1102 76-77 -0.9850759896302534 1102 78-79 -1.0531181660020792 1102 80-81 -1.087947887364166 1102 82-83 -1.0689797326962278 1102 84-85 -1.1385775821790176 1102 86-87 -1.181849599378527 1102 88-89 -1.2448316476134558 1102 90-91 -1.2263212481058225 1102 92-93 -1.2912561063354389 1102 94-95 -1.3608338348828646 1102 96-97 -1.3934987531649519 1102 98-99 -1.4285240214649981 1102 100 -1.4046495259822827 1103 1 -0.5485916844703631 1103 2 -0.5679072962747185 1103 3 -0.6452389601770463 1103 4 -0.4837511804362933 1103 5 -0.47301199647180425 1103 6 -0.4558839587582 1103 7 -0.5103171958393915 1103 8 -0.5520582071291784 1103 9 -0.6667564947870019 1103 10-11 -0.6982553762867525 1103 12-13 -0.795831269843049 1103 14-15 -0.8805695733622727 1103 16-17 -0.9084826006105899 1103 18-19 -0.980277234925218 1103 20-21 -0.9231073817433071 1103 22-23 -0.9181963212325144 1103 24-25 -0.9900213184645423 1103 26-27 -1.0476415321934383 1103 28-29 -1.1080572373355366 1103 30-31 -1.0294094781681125 1103 32-33 -1.0647455477409338 1103 34-35 -1.0258506926310957 1103 36-37 -1.0285563940118365 1103 38-39 -1.1315100466799564 1103 40-41 -1.1042044721539312 1103 42-43 -1.1590667133123134 1103 44-45 -1.1475884242556873 1103 46-47 -1.2275706295998319 1103 48-49 -1.1647063733906364 1103 50-51 -1.0127697973576346 1103 52-53 -0.9363480589160318 1103 54-55 -1.014742526979127 1103 56-57 -1.0090550992739509 1103 58-59 -0.935894452031981 1103 60-61 -0.9950474460940555 1103 62-63 -0.9494953398808192 1103 64-65 -0.9650927945622172 1103 66-67 -1.020124475371027 1103 68-69 -0.9692733442963188 1103 70-71 -1.077025235913645 1103 72-73 -1.0265411732820304 1103 74-75 -1.0355683885485085 1103 76-77 -1.1328568059659219 1103 78-79 -1.153408795134773 1103 80-81 -1.0461571735357786 1103 82-83 -1.1383197913048733 1103 84-85 -1.2200011334052832 1103 86-87 -1.2201385322061036 1103 88-89 -1.3072490420588352 1103 90-91 -1.3173435625815522 1103 92-93 -1.4546934148712154 1103 94-95 -1.3865889654779302 1103 96-97 -1.592937797602005 1103 98-99 -1.5657750373122212 1103 100 -1.5813417633520466 1104 1 -0.48334679551042115 1104 2 -0.48126339541868646 1104 3 -0.42350366007832463 1104 4 -0.30436263115989703 1104 5 -0.3449916352489808 1104 6 -0.35108556394316537 1104 7 -0.366170144608688 1104 8 -0.3415002954742832 1104 9 -0.4575374726047059 1104 10-11 -0.4727667893846643 1104 12-13 -0.5754981161196255 1104 14-15 -0.7492718494410084 1104 16-17 -0.7250486832692999 1104 18-19 -0.7814423826358379 1104 20-21 -0.8108895836694572 1104 22-23 -0.7211881733781169 1104 24-25 -0.7406705557687872 1104 26-27 -0.6960323637065216 1104 28-29 -0.8259374406526874 1104 30-31 -0.8107680843333966 1104 32-33 -0.7801927270591733 1104 34-35 -0.8202167815355779 1104 36-37 -0.9431775609535364 1104 38-39 -0.9471187660191518 1104 40-41 -0.9354815477856491 1104 42-43 -0.9827828634046512 1104 44-45 -0.9998992060245939 1104 46-47 -1.027077371540372 1104 48-49 -1.0248640672373455 1104 50-51 -1.0099492578668006 1104 52-53 -0.8040234180629611 1104 54-55 -0.7157673608153701 1104 56-57 -0.6825665088239177 1104 58-59 -0.7332833303882964 1104 60-61 -0.713659736308685 1104 62-63 -0.785291898585875 1104 64-65 -0.8167924178731951 1104 66-67 -0.6849835121539627 1104 68-69 -0.8162932828931275 1104 70-71 -0.6947608309477928 1104 72-73 -0.7234831573845355 1104 74-75 -0.8276277213628589 1104 76-77 -0.8869220740921158 1104 78-79 -0.8451397031687726 1104 80-81 -0.8608101366654424 1104 82-83 -0.9017772472747119 1104 84-85 -0.9855826355026593 1104 86-87 -0.9894692638127971 1104 88-89 -0.9328480154615661 1104 90-91 -0.9288588076970861 1104 92-93 -0.9908249005655279 1104 94-95 -0.9920383276554965 1104 96-97 -1.1824056365912945 1104 98-99 -1.2515289452504277 1104 100 -1.1260504740532475 1105 1 -0.42963403239214415 1105 2 -0.4144328106774431 1105 3 -0.44384695091508064 1105 4 -0.3331425034298263 1105 5 -0.33259548210111944 1105 6 -0.26558071970975305 1105 7 -0.304202323521082 1105 8 -0.3174379117337409 1105 9 -0.4142710433969796 1105 10-11 -0.43847033252974654 1105 12-13 -0.5066076826017465 1105 14-15 -0.677225514814431 1105 16-17 -0.683522074916219 1105 18-19 -0.6006985564308351 1105 20-21 -0.6632000249912551 1105 22-23 -0.6907031374451975 1105 24-25 -0.681876851119199 1105 26-27 -0.6951014012632157 1105 28-29 -0.7322677200758463 1105 30-31 -0.6718638336843696 1105 32-33 -0.7625603545703683 1105 34-35 -0.8022126190925007 1105 36-37 -0.7660543339357346 1105 38-39 -0.8518513339904246 1105 40-41 -0.9016817197025588 1105 42-43 -0.8029277596367805 1105 44-45 -0.7913443840655958 1105 46-47 -0.876465288409122 1105 48-49 -0.9030609694114773 1105 50-51 -0.9564476864921616 1105 52-53 -0.7490683495953618 1105 54-55 -0.6923452318123182 1105 56-57 -0.7875873795471549 1105 58-59 -0.7070999712700754 1105 60-61 -0.6896376119265923 1105 62-63 -0.8015213675147166 1105 64-65 -0.8022953775907311 1105 66-67 -0.7629479144612716 1105 68-69 -0.752574718383066 1105 70-71 -0.7903211667980159 1105 72-73 -0.7426893741057157 1105 74-75 -0.748633664002174 1105 76-77 -0.8091805816702404 1105 78-79 -0.857658905092709 1105 80-81 -0.8522340894555533 1105 82-83 -0.8658765569187032 1105 84-85 -0.9004686584246713 1105 86-87 -0.942886202690076 1105 88-89 -0.9450876642899075 1105 90-91 -1.0334172759042453 1105 92-93 -0.8844672335080048 1105 94-95 -1.0250125571246897 1105 96-97 -0.924682950212425 1105 98-99 -1.0123371658766196 1105 100 -0.9698504450081664 1106 1 -0.5006545721069919 1106 2 -0.5363919594744466 1106 3 -0.5919383018649533 1106 4 -0.42524017681003556 1106 5 -0.4538978060745862 1106 6 -0.4586486701567196 1106 7 -0.4970313242820126 1106 8 -0.5145269046214693 1106 9 -0.716844964870937 1106 10-11 -0.8055906813494857 1106 12-13 -0.6453729363712526 1106 14-15 -0.7912504586774673 1106 16-17 -0.8263843727598683 1106 18-19 -0.8485728166730624 1106 20-21 -0.9212983061091862 1106 22-23 -0.8103425882050885 1106 24-25 -0.8755221371096695 1106 26-27 -0.9679759540547508 1106 28-29 -0.919917902079753 1106 30-31 -0.9219577286277314 1106 32-33 -0.9521595344839113 1106 34-35 -0.8559858053460587 1106 36-37 -0.9202937558307696 1106 38-39 -1.0633520553443603 1106 40-41 -1.0047480418162493 1106 42-43 -0.8897430938707274 1106 44-45 -0.9715954548534285 1106 46-47 -0.921933386040898 1106 48-49 -0.9840640946029282 1106 50-51 -0.967353014553133 1106 52-53 -0.8192611468554816 1106 54-55 -0.865865121608806 1106 56-57 -0.9342839012850277 1106 58-59 -0.9152482228692165 1106 60-61 -0.7514843407167078 1106 62-63 -0.8586474365338717 1106 64-65 -0.7770611909133791 1106 66-67 -0.7376541471535418 1106 68-69 -0.7091082163496409 1106 70-71 -0.762129233954191 1106 72-73 -0.804459435856181 1106 74-75 -0.8447465670787153 1106 76-77 -0.8222775897206596 1106 78-79 -0.8520523754227405 1106 80-81 -0.887073033221764 1106 82-83 -0.7917362943249167 1106 84-85 -0.855750374230567 1106 86-87 -0.7855830438229496 1106 88-89 -0.8083340254545348 1106 90-91 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1.157607355214175 2208 100 1.1935202956032214 2209 1 0.5927407947153753 2209 2 0.6377681775445794 2209 3 0.7008664069446411 2209 4 0.5276057916742971 2209 5 0.5120774481108086 2209 6 0.5699448544661294 2209 7 0.6363910396840282 2209 8 0.5849570996884381 2209 9 0.7575138309295255 2209 10-11 0.7946991824101417 2209 12-13 0.8571812437638684 2209 14-15 1.0109232403807766 2209 16-17 1.0131383231464568 2209 18-19 0.9554420828824632 2209 20-21 0.9712100159043331 2209 22-23 1.006741067974474 2209 24-25 1.0460644710377238 2209 26-27 1.0773510983584345 2209 28-29 1.0809954993223485 2209 30-31 1.0898511417028303 2209 32-33 1.0940882650931485 2209 34-35 1.10074454319858 2209 36-37 1.1439160767358132 2209 38-39 1.1515874611223396 2209 40-41 1.159416217644285 2209 42-43 1.2064147788906823 2209 44-45 1.2741305311902096 2209 46-47 1.1979733933038759 2209 48-49 1.1965248474398962 2209 50-51 1.2199442534405307 2209 52-53 1.1235172788184897 2209 54-55 1.0503997759633634 2209 56-57 1.0570178058993989 2209 58-59 1.0991861696348195 2209 60-61 1.0656473861279068 2209 62-63 1.0747137519281083 2209 64-65 1.0778581041403115 2209 66-67 1.083545398114424 2209 68-69 1.0599167924432393 2209 70-71 1.0814369805955337 2209 72-73 1.0602574599541725 2209 74-75 1.069748648332812 2209 76-77 1.0897902627870195 2209 78-79 1.0740097703095302 2209 80-81 1.0614042344369317 2209 82-83 1.0780626051624402 2209 84-85 1.0386853287573636 2209 86-87 0.9953661931433864 2209 88-89 1.0458452869410308 2209 90-91 1.1153466456028873 2209 92-93 1.1242011876932843 2209 94-95 1.190677880914567 2209 96-97 1.1266311169649192 2209 98-99 1.1508744334436578 2209 100 1.2990973696013484 2210 1 0.7669100190281952 2210 2 0.762795425489692 2210 3 0.812135217696504 2210 4 0.695011889522668 2210 5 0.6703158038793191 2210 6 0.7392559739208409 2210 7 0.7362549450045677 2210 8 0.8011810718572647 2210 9 0.9837902711060167 2210 10-11 1.0258768268560203 2210 12-13 1.069356841263989 2210 14-15 1.235937876222522 2210 16-17 1.2402086627670599 2210 18-19 1.2157593083633813 2210 20-21 1.2358059906955958 2210 22-23 1.2729603710404191 2210 24-25 1.2467103267668662 2210 26-27 1.2901878159625255 2210 28-29 1.2698962405493361 2210 30-31 1.3205393453663348 2210 32-33 1.3154108285466677 2210 34-35 1.3015026646057564 2210 36-37 1.37357901171179 2210 38-39 1.32751482369531 2210 40-41 1.3307405769066065 2210 42-43 1.4220288943899249 2210 44-45 1.401253391311343 2210 46-47 1.4338412344847598 2210 48-49 1.361485937788693 2210 50-51 1.3532537163306984 2210 52-53 1.26823179909303 2210 54-55 1.1935696934583788 2210 56-57 1.2045397969595797 2210 58-59 1.2313692183600793 2210 60-61 1.1365561399774933 2210 62-63 1.1207068328149319 2210 64-65 1.1405703998221597 2210 66-67 1.1334379258645058 2210 68-69 1.1736297404181002 2210 70-71 1.1354571196242844 2210 72-73 1.0806455535760477 2210 74-75 1.0305930127670706 2210 76-77 1.0125853894592254 2210 78-79 1.021013007462109 2210 80-81 1.0518167289562648 2210 82-83 1.0508264985753861 2210 84-85 1.0627805795614051 2210 86-87 1.0256587663136187 2210 88-89 1.0109197895227098 2210 90-91 1.0523822874346216 2210 92-93 1.0954499572189071 2210 94-95 1.1255683600612194 2210 96-97 1.1521223880908842 2210 98-99 1.1411956870618702 2210 100 1.2290139694777409 2211 1 0.6522277140648107 2211 2 0.6497356936174974 2211 3 0.6934658329219552 2211 4 0.5424421628788139 2211 5 0.5763948265011294 2211 6 0.6075473816428385 2211 7 0.6593724840135735 2211 8 0.6811549180936254 2211 9 0.807239223465686 2211 10-11 0.8337223326133625 2211 12-13 0.8726107050019962 2211 14-15 1.0469437655172058 2211 16-17 1.0533605440828282 2211 18-19 1.036537615897025 2211 20-21 1.0809506522155203 2211 22-23 1.0754185407573544 2211 24-25 1.144928852506652 2211 26-27 1.1554550416714733 2211 28-29 1.2000162945795338 2211 30-31 1.1829420616316284 2211 32-33 1.1799514937957838 2211 34-35 1.174559306854853 2211 36-37 1.211248373856158 2211 38-39 1.2256405620324173 2211 40-41 1.262141521181121 2211 42-43 1.3175491639223438 2211 44-45 1.4040097584629834 2211 46-47 1.3556077260289143 2211 48-49 1.332115498487866 2211 50-51 1.2905345504199772 2211 52-53 1.2032634709286256 2211 54-55 1.1671860360473119 2211 56-57 1.1694253037756752 2211 58-59 1.1111362945599268 2211 60-61 1.112882583974141 2211 62-63 1.0924777279219953 2211 64-65 1.1172466154020313 2211 66-67 1.085853653936141 2211 68-69 1.0878520549402708 2211 70-71 1.086222416448372 2211 72-73 1.0795104525220438 2211 74-75 1.0728823430456558 2211 76-77 1.0057978063294186 2211 78-79 1.0070657958899005 2211 80-81 1.0341426389683512 2211 82-83 1.0212830517149492 2211 84-85 1.0077772729591032 2211 86-87 1.0687925698063516 2211 88-89 1.0845663629639972 2211 90-91 1.0532230133245868 2211 92-93 1.1071000316480735 2211 94-95 1.1253740841519182 2211 96-97 1.1105254353637228 2211 98-99 1.1248571982513234 2211 100 1.1439115975165883 2212 1 0.65830588832765 2212 2 0.7342819011880515 2212 3 0.758605313831648 2212 4 0.6040323552568196 2212 5 0.5933776876023984 2212 6 0.6568953681532079 2212 7 0.6628772082409711 2212 8 0.6789449719200817 2212 9 0.8403106564524307 2212 10-11 0.9229340367262537 2212 12-13 0.9021540322491077 2212 14-15 1.0884677045569546 2212 16-17 1.1158635092268199 2212 18-19 1.0655210917819105 2212 20-21 1.0709715014456336 2212 22-23 1.1138591501253217 2212 24-25 1.1323525216114874 2212 26-27 1.1825865963200215 2212 28-29 1.1499089362567574 2212 30-31 1.1173478210343646 2212 32-33 1.1747902484163575 2212 34-35 1.2221678822497068 2212 36-37 1.2376920061605006 2212 38-39 1.336704783002439 2212 40-41 1.322644392799063 2212 42-43 1.3975160808700977 2212 44-45 1.375272986133453 2212 46-47 1.3662622861012963 2212 48-49 1.3920923933350977 2212 50-51 1.348695820789743 2212 52-53 1.1872582269631025 2212 54-55 1.1619383344875018 2212 56-57 1.203736537873695 2212 58-59 1.214640689873086 2212 60-61 1.2274306055782276 2212 62-63 1.2364873576728783 2212 64-65 1.2058296374853725 2212 66-67 1.137537449943956 2212 68-69 1.1371305136281222 2212 70-71 1.1718217305477836 2212 72-73 1.1551707380260439 2212 74-75 1.1267610427632988 2212 76-77 1.146007493447243 2212 78-79 1.086759088366847 2212 80-81 1.1047163104488753 2212 82-83 1.1290308786733085 2212 84-85 1.184090481829621 2212 86-87 1.1806755369717372 2212 88-89 1.2345212874125764 2212 90-91 1.2244145448411636 2212 92-93 1.21195234969084 2212 94-95 1.2741553567904695 2212 96-97 1.2794727707240021 2212 98-99 1.309133331479753 2212 100 1.3299130468128304 2213 1 0.5919884667810003 2213 2 0.5879036613458979 2213 3 0.6494818812459009 2213 4 0.4842298640090874 2213 5 0.4897635839016843 2213 6 0.5314074797691504 2213 7 0.6041061914390795 2213 8 0.6213111400325033 2213 9 0.7656169497558309 2213 10-11 0.7726571254553676 2213 12-13 0.8391768319938748 2213 14-15 0.9799598839810102 2213 16-17 0.9698677117960486 2213 18-19 0.9799120527389604 2213 20-21 1.0056017901963514 2213 22-23 0.9819526302184585 2213 24-25 1.0090916073815634 2213 26-27 1.0434739106159157 2213 28-29 1.0202624114072094 2213 30-31 1.0279510578575994 2213 32-33 1.0533932253956095 2213 34-35 1.0493951629215204 2213 36-37 1.0884447262519998 2213 38-39 1.0833737961505747 2213 40-41 1.1673577634910615 2213 42-43 1.2353290252612865 2213 44-45 1.1424676249637145 2213 46-47 1.2079569721153902 2213 48-49 1.2506321237416813 2213 50-51 1.2544836864837023 2213 52-53 1.143763800561267 2213 54-55 1.1425366775765653 2213 56-57 1.1520694984573154 2213 58-59 1.1300969878274358 2213 60-61 1.174602391591641 2213 62-63 1.1864192165014131 2213 64-65 1.1585025728147045 2213 66-67 1.1766516176545565 2213 68-69 1.1553902300613146 2213 70-71 1.1017097714418966 2213 72-73 1.1130109777949713 2213 74-75 1.198493524957989 2213 76-77 1.1780612879657824 2213 78-79 1.2104116708012072 2213 80-81 1.1940450950079757 2213 82-83 1.2377602825658478 2213 84-85 1.2685282299219622 2213 86-87 1.2728169865542824 2213 88-89 1.3238579272978086 2213 90-91 1.31688800624649 2213 92-93 1.395949426523817 2213 94-95 1.4115456249743268 2213 96-97 1.412542600881391 2213 98-99 1.417644857071938 2213 100 1.4714435604014042 2214 1 0.5099729190686695 2214 2 0.5189059620773371 2214 3 0.5913731993007296 2214 4 0.4901025174059086 2214 5 0.4603672013136162 2214 6 0.505366072415633 2214 7 0.4697009934466436 2214 8 0.5290601174320813 2214 9 0.6651357435748935 2214 10-11 0.6901238628196396 2214 12-13 0.7420969730903053 2214 14-15 0.8953352269180712 2214 16-17 0.8676994835591287 2214 18-19 0.914579784305559 2214 20-21 0.8709285317144833 2214 22-23 0.9393445365150086 2214 24-25 0.9445402451690299 2214 26-27 0.9792615053475089 2214 28-29 0.9874949801524906 2214 30-31 1.0025115310676043 2214 32-33 1.0567332871364528 2214 34-35 1.0664557408446385 2214 36-37 1.1425296456226377 2214 38-39 1.1198727747292097 2214 40-41 1.1293461997465002 2214 42-43 1.1891297750199783 2214 44-45 1.193684322590542 2214 46-47 1.1302000936233227 2214 48-49 1.2031086062455714 2214 50-51 1.23550931217423 2214 52-53 1.069746894879735 2214 54-55 0.9996881860025297 2214 56-57 0.954537062182105 2214 58-59 0.9734610478015 2214 60-61 0.9835284030980702 2214 62-63 1.0080433413438143 2214 64-65 1.0005125895328533 2214 66-67 0.9921901702976967 2214 68-69 0.9893549777743331 2214 70-71 1.0015525322319263 2214 72-73 1.0022424691486833 2214 74-75 1.047201395644791 2214 76-77 1.0538695770181405 2214 78-79 1.0752493389518065 2214 80-81 1.0596811959211827 2214 82-83 1.100488379672118 2214 84-85 1.1266524040553811 2214 86-87 1.1785391819194686 2214 88-89 1.281045149079894 2214 90-91 1.3073516555488283 2214 92-93 1.2686298333363908 2214 94-95 1.3072983792353092 2214 96-97 1.3435190133173478 2214 98-99 1.402459020026864 2214 100 1.497966201978322 2215 1 0.4531672654300607 2215 2 0.4198119171767871 2215 3 0.49496556990553486 2215 4 0.3745737864533112 2215 5 0.3765841488634081 2215 6 0.4145075046471618 2215 7 0.4706641402103173 2215 8 0.460534611748983 2215 9 0.5701985208123119 2215 10-11 0.5908317525303062 2215 12-13 0.615457527386738 2215 14-15 0.7229909738822897 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0.9886134235388688 2215 84-85 0.995452440467929 2215 86-87 1.0506946992745192 2215 88-89 1.0187338071389256 2215 90-91 1.058279461364961 2215 92-93 1.101348650402585 2215 94-95 1.198019596045409 2215 96-97 1.212095944002165 2215 98-99 1.276261115889806 2215 100 1.4512228793153277 2216 1 0.3315286489131246 2216 2 0.35706197890885605 2216 3 0.3438682012336365 2216 4 0.22213902843868283 2216 5 0.25222330972827933 2216 6 0.3383521158257352 2216 7 0.2031438069297664 2216 8 0.32254900419653865 2216 9 0.3762907214427642 2216 10-11 0.35793394972560577 2216 12-13 0.4348812816063159 2216 14-15 0.5815378126842248 2216 16-17 0.6033050688089148 2216 18-19 0.6359536682141993 2216 20-21 0.6451638047293997 2216 22-23 0.6197450086304173 2216 24-25 0.6659242669062877 2216 26-27 0.5881250744145063 2216 28-29 0.616310278876675 2216 30-31 0.7699820586696049 2216 32-33 0.7756244991307426 2216 34-35 0.8542452949100365 2216 36-37 0.8593651587907019 2216 38-39 0.9042064547309252 2216 40-41 0.89562454978104 2216 42-43 0.816767561171531 2216 44-45 0.8023855814098368 2216 46-47 0.7736837384500461 2216 48-49 0.8197855854441016 2216 50-51 0.7185682908357904 2216 52-53 0.48643336684868643 2216 54-55 0.5398164875728781 2216 56-57 0.5853174828114476 2216 58-59 0.49900667925996345 2216 60-61 0.5588282799843469 2216 62-63 0.680635379850834 2216 64-65 0.6446817304764423 2216 66-67 0.5953695966101549 2216 68-69 0.6804790887678109 2216 70-71 0.5976313587124977 2216 72-73 0.5459301312668003 2216 74-75 0.6787865305676988 2216 76-77 0.7457705337755058 2216 78-79 0.8272474822929041 2216 80-81 0.8297251361429012 2216 82-83 0.8235952989240864 2216 84-85 0.9389146931824541 2216 86-87 1.0577605313365233 2216 88-89 1.0702656866307372 2216 90-91 1.0243016371487137 2216 92-93 1.0913275975529402 2216 94-95 1.1224524020810875 2216 96-97 1.2449432320909857 2216 98-99 1.37071490849684 2216 100 1.2796542446807706 2301 1 0.7363860116583645 2301 2 0.7426019833510864 2301 3 0.8417371551577197 2301 4 0.6728816409978364 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1.6218622637673938 2311 1 0.6658425874739535 2311 2 0.7009070883823973 2311 3 0.738516972851837 2311 4 0.631853425504616 2311 5 0.6393178523012892 2311 6 0.6304207316541692 2311 7 0.6725386197863656 2311 8 0.6823560201762788 2311 9 0.8400039782987676 2311 10-11 0.6870576437038594 2311 12-13 0.8421365936373135 2311 14-15 1.0852825801944803 2311 16-17 1.0723207611510546 2311 18-19 1.0983329980140155 2311 20-21 1.0751802068187502 2311 22-23 1.1120131024489268 2311 24-25 1.1726673995127328 2311 26-27 1.1886037425478975 2311 28-29 1.225902081299914 2311 30-31 1.234919953474055 2311 32-33 1.2431611479218816 2311 34-35 1.2306587632161552 2311 36-37 1.281997460741529 2311 38-39 1.2884084231383142 2311 40-41 1.297133896625617 2311 42-43 1.3163448781148546 2311 44-45 1.337453329975176 2311 46-47 1.2553512071754582 2311 48-49 1.3549390259983838 2311 50-51 1.406152727753529 2311 52-53 1.2813003552930624 2311 54-55 1.2490078386119805 2311 56-57 1.170573436869219 2311 58-59 1.2324826165720637 2311 60-61 1.2100629264580292 2311 62-63 1.1717766333611195 2311 64-65 1.0603516095082313 2311 66-67 1.1001666567598605 2311 68-69 1.1154050801565631 2311 70-71 1.1143963238610368 2311 72-73 1.0945952945065187 2311 74-75 1.099607301914169 2311 76-77 1.104861331358478 2311 78-79 1.1760991864980141 2311 80-81 1.2026395905611302 2311 82-83 1.2451282030100153 2311 84-85 1.2092623614346323 2311 86-87 1.3226874847685046 2311 88-89 1.3410815765513462 2311 90-91 1.3772370938037888 2311 92-93 1.3892036236493084 2311 94-95 1.3915333029380825 2311 96-97 1.445119629232682 2311 98-99 1.4591195958619885 2311 100 1.5906448835942584 2312 1 0.615351719055024 2312 2 0.6629142046852863 2312 3 0.7121523924327526 2312 4 0.539924584977129 2312 5 0.5885484013104829 2312 6 0.4660854652344 2312 7 0.5571750576754937 2312 8 0.6389584864025863 2312 9 0.8234964790930235 2312 10-11 0.8584242305101881 2312 12-13 0.9199119576913901 2312 14-15 1.089742716806633 2312 16-17 1.1139980475434186 2312 18-19 1.095444852472994 2312 20-21 1.0730485315061458 2312 22-23 1.0950085259892717 2312 24-25 1.091291948446056 2312 26-27 1.1344274341581766 2312 28-29 1.2151013757154558 2312 30-31 1.0857915168320247 2312 32-33 1.2374565029427629 2312 34-35 1.2766757140765606 2312 36-37 1.300638857993171 2312 38-39 1.3247448518994105 2312 40-41 1.350359912444361 2312 42-43 1.377758183811217 2312 44-45 1.3607605407590526 2312 46-47 1.3498670128836707 2312 48-49 1.4405257416402506 2312 50-51 1.3541442511378676 2312 52-53 1.2376629145066786 2312 54-55 1.2014769100192169 2312 56-57 1.1804383717401734 2312 58-59 1.2080802650980615 2312 60-61 1.2247242233962297 2312 62-63 1.1735622291010586 2312 64-65 1.1678037229423168 2312 66-67 1.1590342660235038 2312 68-69 1.1725146555730177 2312 70-71 1.1520994372287348 2312 72-73 1.197260084988713 2312 74-75 1.241493036234111 2312 76-77 1.1718252466172174 2312 78-79 1.15672317589528 2312 80-81 0.9942535290548484 2312 82-83 1.1408994973876254 2312 84-85 1.1637169263953453 2312 86-87 1.2682575625108221 2312 88-89 1.2465963704713658 2312 90-91 1.3047062689385704 2312 92-93 1.4095269631786351 2312 94-95 1.406286250578784 2312 96-97 1.4156940487140197 2312 98-99 1.2811910558480477 2312 100 1.4242833735107574 2313 1 0.5984203680189673 2313 2 0.6187388314917328 2313 3 0.6720549176892874 2313 4 0.5700709756538416 2313 5 0.5697986989129973 2313 6 0.6344863278848862 2313 7 0.640561050120354 2313 8 0.6795886696463782 2313 9 0.8477456279498341 2313 10-11 0.7614396631845608 2313 12-13 0.8881848016603797 2313 14-15 1.047173157178996 2313 16-17 1.0396700836207842 2313 18-19 1.0624726019234245 2313 20-21 1.0533000006705606 2313 22-23 1.1181348453604159 2313 24-25 1.1690192770865266 2313 26-27 1.1773863570751573 2313 28-29 1.1853346762463772 2313 30-31 1.1955129757589162 2313 32-33 1.2747253897903192 2313 34-35 1.2668503670720312 2313 36-37 1.2672593880034029 2313 38-39 1.2904596782582658 2313 40-41 1.2678449033575632 2313 42-43 1.3188186258898398 2313 44-45 1.2777717236695239 2313 46-47 1.2589060444454105 2313 48-49 1.2746013039106074 2313 50-51 1.2770630919562365 2313 52-53 1.1435882797641668 2313 54-55 1.08701898307865 2313 56-57 1.0486964347842829 2313 58-59 1.14036552504394 2313 60-61 1.063918672910937 2313 62-63 1.0811469763770063 2313 64-65 1.0770361961382449 2313 66-67 1.0795582746103278 2313 68-69 1.1186327283784934 2313 70-71 1.0982900506470443 2313 72-73 1.094865475959054 2313 74-75 1.0956270079579085 2313 76-77 1.1417652846706616 2313 78-79 1.1370774419281204 2313 80-81 1.142794507929036 2313 82-83 1.106097115377107 2313 84-85 1.238827336928093 2313 86-87 1.2330034446840337 2313 88-89 1.2277532952850692 2313 90-91 1.2980095923650516 2313 92-93 1.394430083640195 2313 94-95 1.478016516556071 2313 96-97 1.4757028676685238 2313 98-99 1.5590869515125725 2313 100 1.5940140233952853 2314 1 0.4876052359263703 2314 2 0.4453142617086172 2314 3 0.5188470885996423 2314 4 0.39882730193546934 2314 5 0.4495399546756147 2314 6 0.4650122397214034 2314 7 0.5130065837317233 2314 8 0.4985817646700923 2314 9 0.6751318040798537 2314 10-11 0.5839875828676426 2314 12-13 0.4366122473076075 2314 14-15 0.8204136610402983 2314 16-17 0.844618750242752 2314 18-19 0.8520434479598222 2314 20-21 0.8482770376477617 2314 22-23 0.732450895145675 2314 24-25 0.7405982871790542 2314 26-27 0.871988445083467 2314 28-29 0.902101880762757 2314 30-31 0.872284582382612 2314 32-33 0.9296921416156039 2314 34-35 0.910958121470614 2314 36-37 1.0003952235072333 2314 38-39 1.024770830756644 2314 40-41 1.1455719392906119 2314 42-43 1.1483069082567212 2314 44-45 1.2068415262185326 2314 46-47 1.175638151031265 2314 48-49 1.1736501114127549 2314 50-51 1.0752303382673247 2314 52-53 0.9688526787467922 2314 54-55 0.9235350455495706 2314 56-57 0.9817860670977154 2314 58-59 0.9233363602776947 2314 60-61 0.9432284959637158 2314 62-63 0.9272715397677374 2314 64-65 0.9333916682333765 2314 66-67 0.9889249215524032 2314 68-69 0.9795157043177269 2314 70-71 0.975075121624073 2314 72-73 0.9836435727974902 2314 74-75 1.0114389884573924 2314 76-77 1.0136001882670982 2314 78-79 1.0664416444741818 2314 80-81 1.0766150964118886 2314 82-83 0.9663488878119786 2314 84-85 0.9526836979749405 2314 86-87 1.1306532018124429 2314 88-89 1.2156361471285493 2314 90-91 1.249262049768813 2314 92-93 1.332348070579208 2314 94-95 1.3601845591895092 2314 96-97 1.3599581239871235 2314 98-99 1.3147651549544221 2314 100 1.3123676607422041 2315 1 0.4367968111177589 2315 2 0.4928726943809387 2315 3 0.5544161367513532 2315 4 0.41417754807845597 2315 5 0.40479929278796334 2315 6 -0.24821291369163845 2315 7 0.2505191524154924 2315 8 0.3021478956501795 2315 9 0.5995207584113089 2315 10-11 0.6406976077381756 2315 12-13 0.6819453116843661 2315 14-15 0.7882418507999418 2315 16-17 0.7092267681175812 2315 18-19 0.8142216852374773 2315 20-21 0.85428591992995 2315 22-23 0.7402501411483513 2315 24-25 0.8789616121750896 2315 26-27 0.9252896485316313 2315 28-29 1.05207158338726 2315 30-31 1.0704893944081206 2315 32-33 1.1070392030440814 2315 34-35 1.0428807227649273 2315 36-37 1.1208428329921745 2315 38-39 1.1392628986581101 2315 40-41 1.1973543244371356 2315 42-43 1.2412684365097064 2315 44-45 1.2102978038100005 2315 46-47 1.2998666944988528 2315 48-49 1.3281691032461609 2315 50-51 1.2820092368742309 2315 52-53 1.133406675579387 2315 54-55 0.9696548432631218 2315 56-57 0.9210401119608065 2315 58-59 0.9020297154951251 2315 60-61 0.7795918319159298 2315 62-63 1.0450036420504034 2315 64-65 1.0505257220593265 2315 66-67 0.9924066899366082 2315 68-69 1.0280224298226202 2315 70-71 1.0320879435370003 2315 72-73 1.1246320518427382 2315 74-75 1.148293541263179 2315 76-77 1.1118658336056129 2315 78-79 1.083222273097455 2315 80-81 1.1338471391424143 2315 82-83 1.2260034394161217 2315 84-85 1.200597278797808 2315 86-87 1.2060336552074986 2315 88-89 1.3695956933730002 2315 90-91 1.472839504492395 2315 92-93 1.5064825676003615 2315 94-95 1.5655249818789834 2315 96-97 1.6309437968362595 2315 98-99 1.7098541615574625 2315 100 1.6751216453462945 2316 1 0.002149766244460949 2316 2 -0.0873391339411711 2316 3 0.12932784407105657 2316 4 0.08652088499604815 2316 5 0.036993647176792877 2316 6 0.13446918954038978 2316 7 0.1169958264393216 2316 8 0.12094958779741916 2316 9 0.2843272758326947 2316 10-11 0.16652778126479006 2316 12-13 0.2791601509182442 2316 14-15 0.468187874698593 2316 16-17 0.5083119905976261 2316 18-19 0.45900317011756897 2316 20-21 0.4183815886366844 2316 22-23 0.343408836208809 2316 24-25 0.42104976606643163 2316 26-27 0.4571541770399534 2316 28-29 0.5765049404359885 2316 30-31 0.5636836322411796 2316 32-33 0.7463059661892828 2316 34-35 0.7242146867121377 2316 36-37 0.7359608743431991 2316 38-39 0.7862801994420678 2316 40-41 0.7496147147440126 2316 42-43 0.8212767975334572 2316 44-45 0.6892767516819447 2316 46-47 0.71891967212823 2316 48-49 0.7363733662229279 2316 50-51 0.47012777409257467 2316 52-53 0.23330007421090926 2316 54-55 0.17252139094858165 2316 56-57 0.3031306946588117 2316 58-59 0.3645807170662678 2316 60-61 0.48779185037620465 2316 62-63 0.5143531294745074 2316 64-65 0.5478898773196974 2316 66-67 0.5333352823471458 2316 68-69 0.5308222105899745 2316 70-71 0.4114371580993321 2316 72-73 0.4683170197865323 2316 74-75 0.621246606343071 2316 76-77 0.7640977587026967 2316 78-79 0.7608999229558364 2316 80-81 0.86701973667871 2316 82-83 0.9128625679562496 2316 84-85 0.9675936534417389 2316 86-87 1.012491737575374 2316 88-89 1.0709753128217798 2316 90-91 1.0852491063971677 2316 92-93 1.1871744136820652 2316 94-95 1.2734284231712572 2316 96-97 1.316668612031357 2316 98-99 1.379612150006988 2316 100 1.246170236301566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 41.0 1 497.0 2 243.0 3 187.0 4 155.0 5 116.0 6 167.0 7 850.0 8 3001.0 9 5516.0 10 7736.0 11 10635.0 12 14357.0 13 17112.0 14 20838.0 15 25231.0 16 32213.0 17 41094.0 18 50628.0 19 60902.0 20 73194.0 21 85431.0 22 96024.0 23 100292.0 24 101523.0 25 106746.0 26 117489.0 27 135269.0 28 158946.0 29 187995.0 30 225496.0 31 277163.0 32 346065.0 33 444777.0 34 610408.0 35 807462.0 36 1145704.0 37 1642736.0 38 1455086.0 39 362835.0 40 372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.13031791533743 18.117538969979716 12.115778232014357 23.6363648826685 2 16.675419028853113 20.17460962561961 40.02769551825781 23.12227582726947 3 18.875795662356737 26.945462659410392 30.13935428936222 24.039387388870654 4 12.938761991034836 16.44150381700713 37.18586438921329 33.43386980274473 5 12.904885385711932 37.565753931667224 33.71377704357416 15.81558363904669 6 29.941843555857673 35.918521669805074 19.547488346470523 14.592146427866734 7 27.316479545356298 33.11363389314791 22.496178623583614 17.07370793791217 8 26.758950406566562 34.53636219928936 20.645328470699766 18.059358923444314 9 28.759105208622948 15.292588040299778 20.063948859159012 35.88435789191826 10-11 25.40012031464275 25.400143123105828 28.810903585775506 20.38883297647592 12-13 25.50283789285458 23.80237158206967 28.07767969044901 22.617110834626743 14-15 23.415465236698115 25.150514854580504 26.213921328546352 25.220098580175033 16-17 22.52893353106342 27.347447160085096 27.046616422367702 23.077002886483783 18-19 22.173801210848957 26.894603740039045 28.851051395009687 22.08054365410231 20-21 23.120570642764392 26.52094765227605 28.32577265799687 22.032709046962687 22-23 23.004773070902136 26.080038201442257 28.412128843896127 22.50305988375948 24-25 22.348521126728446 25.83143152415184 28.77276527159614 23.04728207752358 26-27 22.37907722028676 26.05366053100719 28.510903903450586 23.05635834525546 28-29 22.27623630978034 27.25949336781114 28.29045840430699 22.17381191810153 30-31 23.635534458270616 26.200781381158322 28.543397095487606 21.620287065083456 32-33 23.410054881898393 26.18203372363983 28.189356859131294 22.218554535330487 34-35 23.225722148690522 26.541786428065166 28.329912284942584 21.902579138301725 36-37 22.77096186802549 26.4409948086332 28.372615397596213 22.415427925745096 38-39 22.80327421748136 26.641663802887468 28.249954221339763 22.30510775829141 40-41 22.684748027660216 25.856300612603306 28.88232321202414 22.576628147712334 42-43 22.427221259105696 26.41495126798897 28.083468688152813 23.074358784752512 44-45 21.646548470123765 26.71312383039819 28.8542378216013 22.78608987787675 46-47 21.610049335588116 25.99987945650966 28.527358268881436 23.86271293902079 48-49 22.07672356203619 26.13925114511843 28.44792234246197 23.33610295038341 50-51 21.97002034139728 26.54389004664913 28.416696536329656 23.069393075623942 52-53 21.613187630777176 26.863536255869146 27.989018252582014 23.53425786077166 54-55 21.425753429469065 26.290076852973293 28.679342582436206 23.604827135121432 56-57 21.967485321295 26.232900028567663 28.098528223541695 23.70108642659564 58-59 21.5264500880666 26.36159379332336 28.52699773868877 23.58495837992127 60-61 22.18190471271323 26.37641936503799 28.156427148050433 23.285248774198344 62-63 22.256696088669305 26.170136189382504 28.045994809384293 23.527172912563902 64-65 21.90694685167006 25.882753965810174 27.953469378028267 24.256829804491503 66-67 21.978633096043655 25.81078028505093 27.71567907841187 24.494907540493546 68-69 21.44498208104993 26.089193067101057 28.093264635709787 24.372560216139227 70-71 21.829609468123653 25.99657144584005 28.07871248753778 24.095106598498518 72-73 22.017069259224943 26.609968709684317 27.332864810155456 24.040097220935287 74-75 22.481411010154996 26.53263495456975 27.156404061998927 23.829549973276322 76-77 22.345187177298705 26.244088108812775 27.259048747891036 24.15167596599749 78-79 22.127380485131134 26.47145926129249 27.56203377578939 23.839126477786984 80-81 22.38331976823434 26.605243761607937 27.156558572866075 23.854877897291647 82-83 22.9923889800686 26.37518548690062 27.306613338410767 23.32581219462001 84-85 23.132328331123343 26.83314516760658 26.610823456770277 23.423703044499796 86-87 22.72029468676278 27.002139637039807 26.98497009723991 23.29259557895751 88-89 22.921318209725754 26.241458196397495 26.98713976491406 23.850083828962692 90-91 23.875805402673976 25.97965195294186 26.830466841672244 23.314075802711915 92-93 23.960200926784314 26.143558646078098 26.619127630602467 23.27711279653512 94-95 23.760070842588625 25.969534790795045 26.572247508260855 23.69814685835547 96-97 23.845843579709083 25.746696764192876 26.65402943031814 23.753430225779905 98-99 23.876822916265795 25.988007517294502 26.276853110416575 23.858316456023125 100 24.249642784777016 26.000946644571453 26.159784983288908 23.589625587362626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3293.0 1 2380.5 2 3765.0 3 5596.0 4 3850.0 5 2811.5 6 3777.0 7 6246.0 8 9759.5 9 11445.0 10 9817.5 11 8155.0 12 8399.0 13 9169.5 14 10064.5 15 10948.5 16 11670.5 17 12554.0 18 13599.5 19 14559.5 20 15630.5 21 17081.5 22 19018.0 23 21806.5 24 25489.0 25 29823.5 26 35814.5 27 43128.0 28 52003.5 29 63293.0 30 76589.5 31 90120.5 32 107306.0 33 124750.0 34 140829.5 35 157193.5 36 175782.5 37 200536.5 38 215690.5 39 219340.0 40 222383.0 41 227303.0 42 235499.0 43 249962.5 44 269734.0 45 294668.0 46 321373.5 47 345924.5 48 372296.5 49 397501.0 50 414410.5 51 420150.0 52 407888.0 53 385128.0 54 360800.5 55 324644.0 56 289524.5 57 254961.5 58 216130.0 59 179949.0 60 141274.5 61 108214.5 62 84559.5 63 65575.0 64 48805.0 65 35040.5 66 25741.5 67 19628.0 68 15158.5 69 11418.5 70 8762.0 71 6669.0 72 4992.5 73 3882.0 74 3009.5 75 2274.0 76 1647.0 77 1150.0 78 835.0 79 575.5 80 395.5 81 283.5 82 207.5 83 161.0 84 125.5 85 101.5 86 80.0 87 56.5 88 43.0 89 41.5 90 36.5 91 23.0 92 17.0 93 16.5 94 16.0 95 12.5 96 10.0 97 7.5 98 4.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22096243137101124 2 0.14809863332501952 3 0.02290102789023739 4 0.02185229988331761 5 0.026605773566856183 6 0.05473904227422596 7 0.061589971971604096 8 0.053781508007038334 9 0.04160714375279566 10-11 0.04396678176836517 12-13 0.042199903061054665 14-15 0.020552789092134403 16-17 0.018842906472156497 18-19 0.018934100211888655 20-21 0.02201188892784888 22-23 0.0248787921206785 24-25 0.022068885015181477 26-27 0.019697847782145454 28-29 0.019059491604020366 30-31 0.017378107027708763 32-33 0.02559124321233596 34-35 0.026400587652458837 36-37 0.025101076861275627 38-39 0.02359638015569507 40-41 0.02478759838094635 42-43 0.03055560241900514 44-45 0.04014804391708118 46-47 0.04459943833775699 48-49 0.040695206355474106 50-51 0.028332755013033863 52-53 0.043317026372773564 54-55 0.04155584727419632 56-57 0.04419476611769555 58-59 0.04751193840045269 60-61 0.0244969183355501 62-63 0.026007314649863916 64-65 0.02679386065505375 66-67 0.03192920812372072 68-69 0.0363008080221309 70-71 0.040233538048080074 72-73 0.04382429155003367 74-75 0.025300563166939717 76-77 0.018808708819756943 78-79 0.01829004442503031 80-81 0.021231042531392307 82-83 0.01934447204068335 84-85 0.011501810423718032 86-87 0.01350237308909218 88-89 0.01934447204068335 90-91 0.020108219610940146 92-93 0.02023361100307186 94-95 0.02395545550589043 96-97 0.024559614031615957 98-99 0.029883048588480496 100 0.02974055837014901 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8772532.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.317060173342725 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99939068497163 40.93428747311273 2 14.881545421071834 16.46406687059527 3 4.9096022386073805 8.147542873806678 4 2.149910041796928 4.757068125974177 5 1.1386349564508007 3.1492969200730205 6 0.6843983883744578 2.2715344099349197 7 0.4544226853224147 1.7596128919678395 8 0.32595429857518654 1.442466683843465 9 0.22597667096769924 1.12503285951227 >10 1.1397897490228577 11.004994694548301 >50 0.05853800629586688 2.2282672802900594 >100 0.027883441138659964 2.8791745445776846 >500 0.0025134812882999435 0.9671756016494476 >1k 0.0012043102075504873 1.1479739889753262 >5k 1.570839389880092E-4 0.5806126759763254 >10k+ 7.85419694940046E-5 1.1408921051623298 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34533 0.39364917677131306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27119 0.3091353784745385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17126 0.19522299833161055 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8832 0.10067788866429897 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2798434933038716E-5 2 0.0 0.0 0.0 0.0 2.2798434933038716E-5 3 0.0 0.0 0.0 0.0 2.2798434933038716E-5 4 0.0 0.0 0.0 0.0 2.2798434933038716E-5 5 0.0 0.0 0.0 0.0 2.2798434933038716E-5 6 0.0 0.0 0.0 0.0 2.2798434933038716E-5 7 0.0 0.0 0.0 0.0 2.2798434933038716E-5 8 0.0 0.0 0.0 0.0 2.2798434933038716E-5 9 0.0 0.0 0.0 0.0 2.2798434933038716E-5 10-11 0.0 0.0 0.0 5.1296478599337114E-5 2.2798434933038716E-5 12-13 0.0 0.0 0.0 1.0259295719867423E-4 7.409491353237583E-5 14-15 0.0 5.699608733259679E-6 0.0 1.310910008649726E-4 1.3109100086497263E-4 16-17 0.0 1.1399217466519358E-5 0.0 1.880870881975694E-4 2.1088552313060813E-4 18-19 0.0 1.1399217466519358E-5 0.0 2.963796541295033E-4 2.1658513186386782E-4 20-21 0.0 1.1399217466519358E-5 0.0 6.55455004324863E-4 2.3938356679690652E-4 22-23 0.0 1.1399217466519358E-5 0.0 0.0020689579701732635 2.678816104632049E-4 24-25 0.0 1.1399217466519358E-5 0.0 0.006092881735854597 2.9068004539624365E-4 26-27 0.0 1.1399217466519358E-5 0.0 0.012630332952903448 3.0777887159602266E-4 28-29 0.0 1.1399217466519358E-5 0.0 0.03446553401002128 3.0777887159602266E-4 30-31 0.0 1.1399217466519358E-5 0.0 0.10369298168419334 3.0777887159602266E-4 32-33 0.0 1.1399217466519358E-5 0.0 0.26116177176669175 3.1917808906254205E-4 34-35 0.0 1.1399217466519358E-5 0.0 0.4887756465294171 3.419765239955807E-4 36-37 0.0 2.2798434933038716E-5 0.0 0.8350382762924091 3.419765239955807E-4 38-39 0.0 3.4197652399558074E-5 0.0 1.366281707493344 3.6477495892861945E-4 40-41 0.0 3.4197652399558074E-5 0.0 1.9807052285474707 3.6477495892861945E-4 42-43 0.0 3.4197652399558074E-5 0.0 2.5374088119598763 3.704745676618792E-4 44-45 0.0 4.559686986607743E-5 0.0 3.1592532235847073 3.7617417639513885E-4 46-47 0.0 4.559686986607743E-5 0.0 3.8505473676243076 3.7617417639513885E-4 48-49 0.0 4.559686986607743E-5 0.0 4.573177960479368 4.103718287946969E-4 50-51 0.0 4.559686986607743E-5 0.0 5.318037027394143 4.3317026372773564E-4 52-53 0.0 4.559686986607743E-5 0.0 6.060992424992009 4.673679161272937E-4 54-55 0.0 6.269569606585647E-5 0.0 6.875677398497948 5.015655685268517E-4 56-57 0.0 6.839530479911615E-5 0.0 7.709250875345909 5.243640034598905E-4 58-59 0.0 6.839530479911615E-5 0.0 8.641381986409398 5.414628296596695E-4 60-61 0.0 6.839530479911615E-5 0.0 9.544963757327988 5.585616558594485E-4 62-63 5.699608733259679E-6 6.839530479911615E-5 0.0 10.492227329578279 5.69960873325968E-4 64-65 1.1399217466519358E-5 6.839530479911615E-5 0.0 11.357764212202362 5.87059699525747E-4 66-67 1.1399217466519358E-5 7.979452226563551E-5 0.0 12.302770739394283 6.04158525725526E-4 68-69 1.1399217466519358E-5 7.979452226563551E-5 0.0 13.349241701255693 6.155577431920453E-4 70-71 1.1399217466519358E-5 7.979452226563551E-5 0.0 14.401343876545564 6.21257351925305E-4 72-73 1.1399217466519358E-5 7.979452226563551E-5 0.0 15.34646439591215 7.181507003907196E-4 74-75 1.1399217466519358E-5 8.54941309988952E-5 0.0 16.266649127070725 7.409491353237582E-4 76-77 1.1399217466519358E-5 9.119373973215486E-5 0.0 17.247825371283913 7.46648744057018E-4 78-79 1.1399217466519358E-5 9.119373973215486E-5 0.0 18.267006606530472 7.63747570256797E-4 80-81 2.2798434933038716E-5 9.689334846541455E-5 0.0 19.247698384001332 7.979452226563551E-4 82-83 2.2798434933038716E-5 1.1969178339845326E-4 0.0 20.23493901190671 8.435420925224325E-4 84-85 2.2798434933038716E-5 1.2539139213171293E-4 0.0 21.205901557269897 8.435420925224325E-4 86-87 2.2798434933038716E-5 1.310910008649726E-4 0.0 22.156225819409947 8.606409187222116E-4 88 2.2798434933038716E-5 1.5958904453127102E-4 0.0 22.876154797725444 8.777397449219906E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14150 0.0 55.901707 1 TACCTGG 9220 0.0 51.709774 2 GTATCAA 27180 0.0 43.17726 1 ACCTGGG 10920 0.0 43.176704 3 ATCAACG 32965 0.0 34.965405 3 CAACGCA 33305 0.0 34.636288 5 TATCAAC 33790 0.0 34.528877 2 GTACATG 33115 0.0 33.859695 1 TCAACGC 34525 0.0 33.46644 4 CCTGGGG 10725 0.0 33.354053 4 AACGCAG 35495 0.0 33.043194 6 TACATGG 33725 0.0 32.697495 2 ACATGGG 34370 0.0 30.622862 3 ATGGGGG 22100 0.0 30.586716 5 CATGGGG 27120 0.0 30.45393 4 TGGGGGG 24800 0.0 30.157623 6 GTACCTG 16415 0.0 29.877254 1 ACGCAGA 39480 0.0 29.172592 7 CGCAGAG 40290 0.0 28.490316 8 GGGTTAG 3245 0.0 28.455889 1 >>END_MODULE