FastQCFastQC Report
Mon 6 Jun 2016
ERR1211394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8772532
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319740.36447857927448996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263560.3004377755475842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173480.19775362460917784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146750.054.884741
TACCTGG92100.053.936122
ACCTGGG109050.044.3895573
GTATCAA268250.041.225881
CCTGGGG101300.035.3060724
ATCAACG332400.032.617893
CAACGCA335750.032.2592935
TATCAAC342650.032.053272
CTGGGGG72650.032.0161825
TCAACGC348300.031.1697084
AACGCAG359400.030.6575556
GTACATG321950.030.6400431
TGGGGGG209700.030.5624856
TACATGG328300.029.8612352
GTACCTG170600.029.4901581
ACATGGG327350.028.5125583
ATGGGGG201450.028.247295
GGGTTAG32600.028.1247941
CATGGGG253850.028.0485044
ACGCAGA403950.026.9391377
Produced by FastQC (version 0.11.5)