FastQCFastQC Report
Mon 6 Jun 2016
ERR1211393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558278
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134860.37900355171799394No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128720.3617480140674787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119990.33721367470444974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77880.21886991404269146No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36780.10336460501399833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG36850.055.947442
ACCTGGG44100.045.0934753
CCTGGGG40250.034.338974
GTACCTG62850.033.367571
GTACATG105750.030.1701971
TACATGG107100.029.0945822
ACATGGG108150.027.820533
CTGGGGG32800.027.0894345
CATGGGG78100.027.08744
ATGGGGG59250.025.3906355
TGGGGGG69500.024.9608636
GTACTAG13250.023.8302571
GAGTACA74700.021.9547481
TAACGCC5850.021.6977024
CTAGTAC16500.021.369253
CTGTGCG15300.021.2022449
AGAGTAC147550.020.83802610-11
TAGTACT17050.020.6796264
AGTACAT75950.020.3897422
TCTAACG6050.020.2284032
Produced by FastQC (version 0.11.5)