FastQCFastQC Report
Mon 6 Jun 2016
ERR1211393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558278
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128650.3615512896968702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120470.3385626418171936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113040.31768175505117924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79770.2241814720491204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG37050.053.8999022
ACCTGGG41200.046.988693
CCTGGGG40500.035.271024
GTACCTG65400.030.9870241
GTACATG106750.029.6433661
CTGGGGG29350.029.2945985
TACATGG110100.028.1672132
TGGGGGG62450.027.8356676
TAACGCA3900.027.711674
ACATGGG110650.026.9659443
CATGGGG77500.026.374674
ATGGGGG58900.026.084175
TCGTGAT6500.023.8554632
TATCACG2756.002665E-1122.2125232
ATAACGC4500.021.9280553
CTAACGC5150.021.8976423
GAGTACA80150.021.5299421
CGTTAAC4100.020.6428181
GAGTACT115000.020.63097612-13
AGTACAT80350.019.8829272
Produced by FastQC (version 0.11.5)