##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211393_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3558278 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05583262465721 33.0 31.0 34.0 30.0 34.0 2 32.180632598127524 33.0 31.0 34.0 30.0 34.0 3 32.26135900567634 34.0 31.0 34.0 30.0 34.0 4 35.90607872684484 37.0 35.0 37.0 35.0 37.0 5 35.77163195230952 37.0 35.0 37.0 35.0 37.0 6 35.815785332118516 37.0 35.0 37.0 35.0 37.0 7 35.738956034351446 37.0 35.0 37.0 33.0 37.0 8 35.72876936540653 37.0 35.0 37.0 33.0 37.0 9 37.31588285119937 39.0 37.0 39.0 34.0 39.0 10-11 37.29241841137764 39.0 37.0 39.0 34.0 39.0 12-13 37.20520403408615 39.0 37.0 39.0 33.0 39.0 14-15 38.40005727489533 40.0 38.0 41.0 33.5 41.0 16-17 38.396292673028924 40.0 38.0 41.0 33.5 41.0 18-19 38.41220640995448 40.0 38.0 41.0 33.5 41.0 20-21 38.36509246888524 40.0 38.0 41.0 34.0 41.0 22-23 38.25480836516989 40.0 38.0 41.0 34.0 41.0 24-25 38.13337603188958 40.0 37.0 41.0 33.0 41.0 26-27 38.004804009130254 40.0 37.0 41.0 33.0 41.0 28-29 37.93230953286955 40.0 37.0 41.0 33.0 41.0 30-31 37.98189615875994 40.0 37.0 41.0 33.0 41.0 32-33 37.810709702839404 40.0 37.0 41.0 33.0 41.0 34-35 37.68002317413086 40.0 37.0 41.0 33.0 41.0 36-37 37.52254924994618 40.0 36.5 41.0 32.0 41.0 38-39 37.403938927762255 40.0 36.0 41.0 32.0 41.0 40-41 37.34339180356341 40.0 36.0 41.0 32.0 41.0 42-43 37.20711844324698 40.0 36.0 41.0 31.0 41.0 44-45 37.045904226707414 39.0 36.0 41.0 31.0 41.0 46-47 36.82759272884243 39.0 35.5 41.0 30.5 41.0 48-49 36.59910088531588 39.0 35.0 41.0 30.0 41.0 50-51 36.421060833358155 39.0 35.0 41.0 30.0 41.0 52-53 36.22746466127717 39.0 35.0 40.0 29.0 41.0 54-55 36.03355344916839 38.5 35.0 40.0 29.0 41.0 56-57 35.79864979633407 38.0 35.0 40.0 28.0 41.0 58-59 35.53083443733176 38.0 34.0 40.0 28.0 41.0 60-61 35.262806334974385 38.0 34.0 40.0 27.0 41.0 62-63 34.98127479078363 37.0 34.0 40.0 27.0 41.0 64-65 34.634595160917726 36.5 34.0 39.5 26.0 41.0 66-67 34.27291923228033 36.0 33.0 39.0 26.0 40.5 68-69 33.896813290024 35.5 33.0 39.0 26.0 40.0 70-71 33.50835516505455 35.0 32.5 38.0 25.0 40.0 72-73 33.14506103795151 35.0 32.0 37.5 25.0 40.0 74-75 32.778779791798165 35.0 32.0 37.0 24.0 39.0 76-77 31.758279426171875 34.0 30.5 36.0 23.5 38.5 78-79 32.24392234108746 35.0 31.5 36.0 24.0 38.5 80-81 32.13017715310608 35.0 31.5 35.5 24.0 37.5 82-83 31.858466792083135 34.0 31.0 35.0 24.0 37.0 84-85 31.5886591772762 34.0 31.0 35.0 23.0 36.5 86-87 31.356343433537234 34.0 31.0 35.0 23.0 36.0 88-89 31.123059946412283 34.0 31.0 35.0 21.5 36.0 90-91 30.892729573124978 34.0 30.5 35.0 20.0 35.0 92-93 30.720973600151535 34.0 30.0 35.0 20.0 35.0 94-95 30.55895604559284 34.0 30.0 35.0 20.0 35.0 96-97 30.400248940639266 34.0 30.0 35.0 19.0 35.0 98-99 30.210919720156774 34.0 29.0 35.0 19.0 35.0 100 28.32089117264025 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17915143853533166 1101 2 -0.1575748584462744 1101 3 -0.26743348534792943 1101 4 -0.07553743963883619 1101 5 -0.13076338033921076 1101 6 -0.23094312001409634 1101 7 -0.24764904260751308 1101 8 -0.36640859256930014 1101 9 -0.452238259359234 1101 10-11 -0.44273844989518807 1101 12-13 -0.36237909279666525 1101 14-15 -0.5084272441791668 1101 16-17 -0.5446217904905595 1101 18-19 -0.5540678571890965 1101 20-21 -0.5334055426954691 1101 22-23 -0.648786016537457 1101 24-25 -0.6787004421302569 1101 26-27 -0.5445320025233329 1101 28-29 -0.574645648072476 1101 30-31 -0.5977892954947919 1101 32-33 -0.45395202007576074 1101 34-35 -0.4871719576703981 1101 36-37 -0.5209912993805474 1101 38-39 -0.5088118151492367 1101 40-41 -0.5387543720871051 1101 42-43 -0.5200748297779398 1101 44-45 -0.5506320466156751 1101 46-47 -0.6199230962784483 1101 48-49 -0.453926463576515 1101 50-51 -0.5159903991554273 1101 52-53 -0.41026552517369197 1101 54-55 -0.5309510118653122 1101 56-57 -0.4283732854686093 1101 58-59 -0.506212803124356 1101 60-61 -0.5434209917910238 1101 62-63 -0.5555326932399325 1101 64-65 -0.6650157967434112 1101 66-67 -0.6339120952626942 1101 68-69 -0.7114270246133927 1101 70-71 -0.7467949775747229 1101 72-73 -0.7870225028854705 1101 74-75 -0.7348526420619876 1101 76-77 -0.6888276777935545 1101 78-79 -0.6066825641110754 1101 80-81 -0.43194202796236425 1101 82-83 -0.48768492745404046 1101 84-85 -0.50836030581711 1101 86-87 -0.4806745523525322 1101 88-89 -0.4475081146797031 1101 90-91 -0.7788204267081227 1101 92-93 -0.6833216058261122 1101 94-95 -0.6197146730066585 1101 96-97 -0.7817739206580434 1101 98-99 -0.8923788306083686 1101 100 -0.7741672945616855 1102 1 -0.2591181728577183 1102 2 -0.20869001468446413 1102 3 -0.29459560900087567 1102 4 -0.19384751995334426 1102 5 -0.1963582175680827 1102 6 -0.2190457938689363 1102 7 -0.2835416619306841 1102 8 -0.36632659151084823 1102 9 -0.3468777551787312 1102 10-11 -0.3841171920416002 1102 12-13 -0.40485418715604027 1102 14-15 -0.5866875616764133 1102 16-17 -0.5346190183507602 1102 18-19 -0.5577629450736765 1102 20-21 -0.5679805232614186 1102 22-23 -0.4646486657338258 1102 24-25 -0.6051830038200592 1102 26-27 -0.5317589322137266 1102 28-29 -0.7079460258976837 1102 30-31 -0.5312153888907005 1102 32-33 -0.4867831696220435 1102 34-35 -0.5303266139929121 1102 36-37 -0.3969365893599317 1102 38-39 -0.5179307011755228 1102 40-41 -0.6236848844289398 1102 42-43 -0.5887973385838521 1102 44-45 -0.5695959060546159 1102 46-47 -0.6585672097462023 1102 48-49 -0.6627402203687396 1102 50-51 -0.7409924831066732 1102 52-53 -0.7606129150005216 1102 54-55 -0.8299707793545039 1102 56-57 -0.7264729157853509 1102 58-59 -0.7574037570312413 1102 60-61 -0.8643040249384484 1102 62-63 -0.7798865229607586 1102 64-65 -0.7064578328510152 1102 66-67 -0.7094124858823889 1102 68-69 -0.9549389574151732 1102 70-71 -0.9506606586778332 1102 72-73 -0.9571221642191645 1102 74-75 -0.8743409981055379 1102 76-77 -0.8025873778596591 1102 78-79 -0.6323290251741405 1102 80-81 -0.6121834561377746 1102 82-83 -0.5793845011648528 1102 84-85 -0.7465339309423911 1102 86-87 -0.785150007283935 1102 88-89 -0.7667109015525533 1102 90-91 -0.6924903714785664 1102 92-93 -0.7458638906508774 1102 94-95 -0.8029431302817791 1102 96-97 -0.8942210222211031 1102 98-99 -0.9123725496762312 1102 100 -0.880491262715033 1103 1 -0.41063907420242884 1103 2 -0.29371494073129867 1103 3 -0.36043455020368853 1103 4 -0.17205107677648357 1103 5 -0.17785480177647628 1103 6 -0.2599470992945285 1103 7 -0.24836175833496554 1103 8 -0.29350069670701373 1103 9 -0.4242824606821003 1103 10-11 -0.5159321775259613 1103 12-13 -0.5798674456915833 1103 14-15 -0.6994783164504739 1103 16-17 -0.751386155253492 1103 18-19 -0.7470196028227463 1103 20-21 -0.6758550130533294 1103 22-23 -0.7549179809648408 1103 24-25 -0.77149512742497 1103 26-27 -0.7682815817209061 1103 28-29 -0.6989802113129002 1103 30-31 -0.6773531283791385 1103 32-33 -0.5475282796777066 1103 34-35 -0.6793307506560069 1103 36-37 -0.6705770493034464 1103 38-39 -0.6590646693471811 1103 40-41 -0.71871430855672 1103 42-43 -0.5961442368722203 1103 44-45 -0.6051487182391142 1103 46-47 -0.6762755062502706 1103 48-49 -0.6304137500462517 1103 50-51 -0.7893022653524184 1103 52-53 -0.8045621122207436 1103 54-55 -0.8637729737894517 1103 56-57 -0.9107024279105289 1103 58-59 -0.9510082323271476 1103 60-61 -0.8074855038446103 1103 62-63 -0.9263069147848668 1103 64-65 -0.8091059061402746 1103 66-67 -0.9152773745303335 1103 68-69 -0.9149483660648485 1103 70-71 -0.9721394862697323 1103 72-73 -1.001779465449161 1103 74-75 -1.1709198048766254 1103 76-77 -0.9685473154757958 1103 78-79 -0.6685249254385255 1103 80-81 -0.6129121845361851 1103 82-83 -0.7171881216289577 1103 84-85 -0.633011010371817 1103 86-87 -0.6624510997872619 1103 88-89 -0.69237883711018 1103 90-91 -0.8799381721330484 1103 92-93 -0.7921607325659181 1103 94-95 -0.9130517324196425 1103 96-97 -1.0655158999086574 1103 98-99 -1.0917446254325576 1103 100 -1.092625474450898 1104 1 -0.36934216969595823 1104 2 -0.30059730068436963 1104 3 -0.3491824876915324 1104 4 -0.22529251460625233 1104 5 -0.28164033501821706 1104 6 -0.3584602868247231 1104 7 -0.28337427456843045 1104 8 -0.33364479023684623 1104 9 -0.5494408741632455 1104 10-11 -0.4234869321262238 1104 12-13 -0.5379317897970353 1104 14-15 -0.6154366317466256 1104 16-17 -0.5923688207406812 1104 18-19 -0.6829762477193242 1104 20-21 -0.7109575191232338 1104 22-23 -0.6912426235150164 1104 24-25 -0.8407792631774811 1104 26-27 -0.8002069956349445 1104 28-29 -0.7284245328954029 1104 30-31 -0.7158244696551037 1104 32-33 -0.5365254047082928 1104 34-35 -0.707083890693518 1104 36-37 -0.624491862061852 1104 38-39 -0.7994256795546519 1104 40-41 -0.8759547849264493 1104 42-43 -0.7488373836854478 1104 44-45 -0.8557666876605978 1104 46-47 -0.828637710048433 1104 48-49 -0.8875719611973025 1104 50-51 -0.9039324561387048 1104 52-53 -0.9365225025766577 1104 54-55 -0.7941112336252019 1104 56-57 -0.8828353791693644 1104 58-59 -1.01144866101599 1104 60-61 -0.9654460949621608 1104 62-63 -0.9734935462555043 1104 64-65 -0.9911151537232925 1104 66-67 -1.0045812100187206 1104 68-69 -1.1115089113836518 1104 70-71 -1.134013753019211 1104 72-73 -1.083039307474845 1104 74-75 -0.9873001509395003 1104 76-77 -0.9066186235006128 1104 78-79 -0.907082884382767 1104 80-81 -0.9718091012765697 1104 82-83 -1.032328019554214 1104 84-85 -1.0497102223823873 1104 86-87 -0.9982637609455338 1104 88-89 -1.0239010024499358 1104 90-91 -0.9186089026544622 1104 92-93 -1.1888780837042994 1104 94-95 -1.1741824360891897 1104 96-97 -1.2332734237647855 1104 98-99 -1.1426618159616275 1104 100 -1.235252503241373 1105 1 -0.31460290518899825 1105 2 -0.3364330986530497 1105 3 -0.35131926922167267 1105 4 -0.1864598920049687 1105 5 -0.2598005133890311 1105 6 -0.3727101779222295 1105 7 -0.4412874735504744 1105 8 -0.3697720456647744 1105 9 -0.45803671981975924 1105 10-11 -0.4877571707946302 1105 12-13 -0.47861202915325407 1105 14-15 -0.587279749109122 1105 16-17 -0.6479601039559455 1105 18-19 -0.6283143612988908 1105 20-21 -0.7261541454386631 1105 22-23 -0.7212373790002644 1105 24-25 -0.7408456077220364 1105 26-27 -0.7179239981798204 1105 28-29 -0.7520141023293405 1105 30-31 -0.7012679320665569 1105 32-33 -0.6817240944393603 1105 34-35 -0.7754527319710078 1105 36-37 -0.859419900441381 1105 38-39 -0.8036169925748027 1105 40-41 -0.7247601532461445 1105 42-43 -0.74606796465158 1105 44-45 -0.7897001310427711 1105 46-47 -0.9835596728152396 1105 48-49 -0.8917545333595385 1105 50-51 -0.8498595029323894 1105 52-53 -0.9109014717859125 1105 54-55 -0.8805292300486229 1105 56-57 -0.9737732588951644 1105 58-59 -1.1362249810288247 1105 60-61 -1.0703859693230768 1105 62-63 -1.088888489740846 1105 64-65 -0.9948401921257499 1105 66-67 -0.879679652139906 1105 68-69 -0.960996496499547 1105 70-71 -0.9720052471614054 1105 72-73 -0.9372670168542143 1105 74-75 -0.9271289447584259 1105 76-77 -0.9337651489379439 1105 78-79 -0.9093837361148935 1105 80-81 -0.8704539817130943 1105 82-83 -0.9375251044064505 1105 84-85 -0.803788047542298 1105 86-87 -0.875796317058974 1105 88-89 -0.8661502974495896 1105 90-91 -0.8871843720815207 1105 92-93 -0.9648354225859279 1105 94-95 -0.8148608126396191 1105 96-97 -0.8287660069319003 1105 98-99 -1.0014131342549497 1105 100 -1.2010261694724313 1106 1 -0.4242358839637923 1106 2 -0.37036637981700693 1106 3 -0.3234530933335833 1106 4 -0.23330026337364274 1106 5 -0.25662977461269776 1106 6 -0.33470188878794715 1106 7 -0.40571182312394427 1106 8 -0.40398742790206654 1106 9 -0.6206196836200064 1106 10-11 -0.5455221960581369 1106 12-13 -0.49268520153439965 1106 14-15 -0.6751349114621519 1106 16-17 -0.7535625153404553 1106 18-19 -0.7575978052506258 1106 20-21 -0.7568848065405902 1106 22-23 -0.7705374398645333 1106 24-25 -0.7365300409597211 1106 26-27 -0.772721165575895 1106 28-29 -0.7242580617918009 1106 30-31 -0.7601343108355465 1106 32-33 -0.7350991632739508 1106 34-35 -0.7244581324675821 1106 36-37 -0.8266555997525487 1106 38-39 -0.8678674442263556 1106 40-41 -0.8868475079689233 1106 42-43 -0.7782429446050187 1106 44-45 -0.9333253230550653 1106 46-47 -1.0034622899789625 1106 48-49 -1.1207413847444414 1106 50-51 -1.0535326134831635 1106 52-53 -1.003902080428638 1106 54-55 -0.9964959575795547 1106 56-57 -1.1531054908749923 1106 58-59 -1.0588954734497733 1106 60-61 -1.1554442734318968 1106 62-63 -1.1082075827665747 1106 64-65 -1.212027103408822 1106 66-67 -1.0394203510163393 1106 68-69 -1.1068176328258588 1106 70-71 -1.1045289890582453 1106 72-73 -0.9892170888286316 1106 74-75 -1.0525972615231503 1106 76-77 -1.164488081243345 1106 78-79 -0.9972079832818075 1106 80-81 -1.011840721030449 1106 82-83 -0.9980956828102592 1106 84-85 -0.8903109585935134 1106 86-87 -0.9045831858336726 1106 88-89 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0.9940265554039556 2311 40-41 0.9735279398017695 2311 42-43 1.0395983226307308 2311 44-45 0.9940493000663011 2311 46-47 0.9949866614483653 2311 48-49 0.9774386400978088 2311 50-51 0.9781602220844832 2311 52-53 0.9819754198460302 2311 54-55 0.9365810787375821 2311 56-57 0.9210363027785533 2311 58-59 1.0895107907584958 2311 60-61 1.0168684980947873 2311 62-63 1.0358419506197123 2311 64-65 1.099890968396899 2311 66-67 1.1214756883074344 2311 68-69 1.170407990332997 2311 70-71 1.1489133826893863 2311 72-73 1.1939102848246463 2311 74-75 1.0484994337854943 2311 76-77 1.0564490732981398 2311 78-79 0.9942375284154892 2311 80-81 0.9399336139775158 2311 82-83 0.9075907860071482 2311 84-85 0.9805533642278164 2311 86-87 1.0344522205294027 2311 88-89 1.0312397089279237 2311 90-91 1.0399086082684477 2311 92-93 1.062263126027613 2311 94-95 1.0081812559628744 2311 96-97 1.196996084181226 2311 98-99 1.1772303105253208 2311 100 1.399415505193975 2312 1 0.10438357063060977 2312 2 0.08979083090193285 2312 3 0.3021935846384949 2312 4 0.2178126163458387 2312 5 0.28193199501189525 2312 6 0.37060203092700306 2312 7 0.42083655197932757 2312 8 0.19660168479883566 2312 9 0.33533024228898256 2312 10-11 0.28059795107835583 2312 12-13 0.35610855381234074 2312 14-15 0.7231459230278645 2312 16-17 0.7492325698825297 2312 18-19 0.780093584313704 2312 20-21 0.8118278273325572 2312 22-23 0.3949610658568119 2312 24-25 0.5027384880222456 2312 26-27 0.7701280136579101 2312 28-29 0.813321446193072 2312 30-31 0.8106928360327217 2312 32-33 0.6281014218325041 2312 34-35 0.8224285549435919 2312 36-37 0.9267060997139822 2312 38-39 0.8854458872211168 2312 40-41 0.9545874685579321 2312 42-43 0.8377902231533554 2312 44-45 0.7785910911452092 2312 46-47 0.8178984929380348 2312 48-49 0.9078863754693955 2312 50-51 0.9344264368816226 2312 52-53 0.9876012708888027 2312 54-55 0.9432199022938903 2312 56-57 0.9288335976663333 2312 58-59 0.9398271711376225 2312 60-61 0.9675726988571398 2312 62-63 0.9619358161958118 2312 64-65 1.0225665832455562 2312 66-67 1.047233642252202 2312 68-69 1.0003038495610994 2312 70-71 0.8391591554517177 2312 72-73 0.9335814456692404 2312 74-75 0.9087886923063948 2312 76-77 0.911010487991561 2312 78-79 0.8303761514216674 2312 80-81 0.7966910555366624 2312 82-83 0.7744634788498246 2312 84-85 0.6392866048308861 2312 86-87 0.825134613646199 2312 88-89 0.47111800354548805 2312 90-91 0.8418579094629948 2312 92-93 1.0439453964827408 2312 94-95 1.1304849097149976 2312 96-97 1.0637018490206671 2312 98-99 1.0644311058775564 2312 100 1.34381411890309 2313 1 -0.4027170350329037 2313 2 -0.08510416370633322 2313 3 0.23940924181788859 2313 4 0.10825763060323368 2313 5 0.23433292586726395 2313 6 0.36370913062900456 2313 7 0.13472269677667015 2313 8 0.041915434219014 2313 9 0.2713016814538989 2313 10-11 0.339735759600849 2313 12-13 0.5384426449667714 2313 14-15 0.5369807008266676 2313 16-17 0.7059508109536878 2313 18-19 0.6490934754936148 2313 20-21 0.8007295126066936 2313 22-23 0.8413847311273273 2313 24-25 0.7021175657472725 2313 26-27 0.8002379154089994 2313 28-29 0.7521544615855902 2313 30-31 0.8547457259680371 2313 32-33 0.9113531292163373 2313 34-35 0.9051848941489098 2313 36-37 0.7453853853730195 2313 38-39 0.4925501891745867 2313 40-41 0.6679993015089281 2313 42-43 0.7205467093392741 2313 44-45 0.9192958936166136 2313 46-47 1.006875181868999 2313 48-49 1.0205090641245746 2313 50-51 1.0138733989586086 2313 52-53 0.9215398050164154 2313 54-55 1.0010003449570064 2313 56-57 1.1322492798759782 2313 58-59 1.009356018285473 2313 60-61 1.0649269609216958 2313 62-63 1.183609537659187 2313 64-65 1.2211308168443367 2313 66-67 1.1902307977420534 2313 68-69 1.214607528142615 2313 70-71 1.0960704963715386 2313 72-73 1.1077595015633577 2313 74-75 0.7159114103849191 2313 76-77 0.9470013285959453 2313 78-79 0.7938911565896589 2313 80-81 0.921392963905582 2313 82-83 0.9385565423942808 2313 84-85 1.0173251770047074 2313 86-87 1.0854458880763502 2313 88-89 0.9809483928301468 2313 90-91 1.132795885772012 2313 92-93 0.8458890625197313 2313 94-95 0.8920694988750917 2313 96-97 1.2069082885565763 2313 98-99 1.2943116253462001 2313 100 1.3770843757832658 2314 1 -0.3073058158089914 2314 2 -0.11196158369165232 2314 3 0.16521358012866472 2314 4 -0.08475425026691141 2314 5 0.20607196306534092 2314 6 0.19598378996659704 2314 7 0.12280218280665167 2314 8 0.1527010477185513 2314 9 0.18170615176292415 2314 10-11 0.4013497262120893 2314 12-13 0.47182925678759347 2314 14-15 0.47922957328977844 2314 16-17 0.6686606291388912 2314 18-19 0.7716824021892705 2314 20-21 0.3682606309806147 2314 22-23 0.6075586137001565 2314 24-25 0.81576994695385 2314 26-27 0.8538693032365288 2314 28-29 0.7738207310027505 2314 30-31 0.7604837179320754 2314 32-33 0.8203724183436165 2314 34-35 0.7779775971324554 2314 36-37 0.7319747125091496 2314 38-39 0.7430198981829577 2314 40-41 0.7887099600566714 2314 42-43 0.7096759724903805 2314 44-45 0.7132749702227059 2314 46-47 0.7879318861129221 2314 48-49 0.6370862725817048 2314 50-51 0.7953167251371269 2314 52-53 0.9296400713331323 2314 54-55 0.9603992007161679 2314 56-57 0.830731467656932 2314 58-59 0.8553797562286576 2314 60-61 0.8383608283974269 2314 62-63 0.5474281606187859 2314 64-65 0.6420271631710506 2314 66-67 0.7701837919568177 2314 68-69 0.8401474859535725 2314 70-71 0.9075673838986518 2314 72-73 0.9112296233975385 2314 74-75 0.8942671370705497 2314 76-77 0.7078118290064737 2314 78-79 0.7205216332582438 2314 80-81 0.7188080188993595 2314 82-83 0.7295802340953443 2314 84-85 0.6161625583078205 2314 86-87 0.9043197990272347 2314 88-89 0.945131684889013 2314 90-91 0.9117316454155606 2314 92-93 1.0136054251810016 2314 94-95 1.057235974002051 2314 96-97 1.0209866488750237 2314 98-99 1.1512493946558138 2314 100 1.3166773013130708 2315 1 -0.07920180661192688 2315 2 0.06594862598939244 2315 3 0.00480511542632911 2315 4 -0.10267604940028718 2315 5 0.20173517772883542 2315 6 0.3418822645864168 2315 7 0.4131249477841621 2315 8 0.3584619748408713 2315 9 0.6252990005036381 2315 10-11 0.5679754315580965 2315 12-13 0.575803691844115 2315 14-15 0.7077261762426801 2315 16-17 0.7108318122915946 2315 18-19 0.7970948319827116 2315 20-21 0.7587202005962723 2315 22-23 0.8279049544088934 2315 24-25 0.7730525717066001 2315 26-27 0.779005033797425 2315 28-29 0.8958225475160688 2315 30-31 0.8612484609387252 2315 32-33 0.8282602988579697 2315 34-35 0.7804086625366722 2315 36-37 0.7322337079855359 2315 38-39 0.741681058237198 2315 40-41 0.7763455847971485 2315 42-43 0.857229346193499 2315 44-45 0.7388203633730726 2315 46-47 0.7966854570229813 2315 48-49 0.8942504737031456 2315 50-51 0.7245987907347882 2315 52-53 0.8202972605819596 2315 54-55 0.7131482300254675 2315 56-57 0.8663926579758083 2315 58-59 0.9129646358452277 2315 60-61 0.986465394209894 2315 62-63 0.9395125498635295 2315 64-65 1.0941605927727522 2315 66-67 1.0205078669911174 2315 68-69 0.9068533449762342 2315 70-71 0.5901033215712701 2315 72-73 0.772800324347763 2315 74-75 1.0098547282295485 2315 76-77 0.9546798885097161 2315 78-79 0.8517733713586324 2315 80-81 0.8258351614136359 2315 82-83 0.8385401601141709 2315 84-85 0.8294702009124819 2315 86-87 0.8258003335727295 2315 88-89 0.9222804531604929 2315 90-91 0.9534217689418512 2315 92-93 0.9487038307635807 2315 94-95 0.9718535517049283 2315 96-97 1.0964217930214843 2315 98-99 0.9240615257393756 2315 100 0.831667239907599 2316 1 0.4955296335183519 2316 2 0.49198464959178523 2316 3 0.4644295804669838 2316 4 0.324818763560053 2316 5 0.3930999207931407 2316 6 0.41190698535042714 2316 7 0.47437438461385995 2316 8 0.4706079536161667 2316 9 0.4650943546947559 2316 10-11 0.40034737271487586 2316 12-13 0.5831659112342891 2316 14-15 0.8081553812266051 2316 16-17 0.7637432873740906 2316 18-19 0.8175045116321513 2316 20-21 0.8695101659712563 2316 22-23 0.896386934637313 2316 24-25 1.0100514130649856 2316 26-27 1.0106664994413066 2316 28-29 0.9314913106724418 2316 30-31 0.8343261939611892 2316 32-33 0.7674718331729764 2316 34-35 0.6934301036989083 2316 36-37 0.6351199714963798 2316 38-39 0.5798032617872835 2316 40-41 0.6626697124809553 2316 42-43 0.771317192789688 2316 44-45 0.6873121471693722 2316 46-47 0.7161518945901477 2316 48-49 0.4136865609007856 2316 50-51 0.5281613200469053 2316 52-53 0.6559172890571858 2316 54-55 0.7227405303863108 2316 56-57 0.8054143713894462 2316 58-59 0.7878136181115565 2316 60-61 0.716188327007842 2316 62-63 0.713691013392328 2316 64-65 0.7520965641901256 2316 66-67 0.7301054844346666 2316 68-69 0.7803272268786827 2316 70-71 0.7016738598572019 2316 72-73 0.7487037669648018 2316 74-75 0.8208334867463236 2316 76-77 0.6760714141495967 2316 78-79 0.5744762343192704 2316 80-81 0.6033827886314 2316 82-83 0.5289994784536844 2316 84-85 0.5407856975133143 2316 86-87 0.5899395261983713 2316 88-89 0.5062115999807766 2316 90-91 0.5742840927859589 2316 92-93 0.6175205250189499 2316 94-95 0.6126665766488628 2316 96-97 0.7262999804395243 2316 98-99 0.8151552968451519 2316 100 0.5054981948514587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 22.0 13 106.0 14 362.0 15 1235.0 16 3092.0 17 6391.0 18 10472.0 19 14633.0 20 19052.0 21 24421.0 22 29319.0 23 30630.0 24 31608.0 25 34075.0 26 39869.0 27 48004.0 28 58480.0 29 71103.0 30 87475.0 31 108562.0 32 134645.0 33 175465.0 34 242020.0 35 334037.0 36 502103.0 37 767762.0 38 678406.0 39 104906.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43063509211369 19.135010288592344 12.233667738359888 24.20068688093408 2 16.17144585105492 21.2376885673351 38.46661222085515 24.12425336075484 3 18.77652617361544 26.069126695553297 31.157852197045877 23.996494933785385 4 12.170227070801557 16.394283078619896 38.348198839222896 33.087291011355646 5 12.128967273097231 38.00687605866319 35.04046914359541 14.823687524644175 6 30.069938324099464 37.34494606660863 18.286823008207904 14.298292601084006 7 26.50670704580565 33.09861272589259 22.826832005301448 17.567848223000315 8 26.31571255854684 34.53865960404265 20.630268372593136 18.515359464817376 9 26.739699540359425 14.84662956004626 20.829404631254718 37.584266268339604 10-11 25.24736183443036 25.423417529239806 29.030823868706207 20.298396767623625 12-13 25.41698300732505 23.874336633112424 28.587598153427713 22.12108220613481 14-15 22.482152884063584 25.46189477044795 26.84445959534359 25.211492750144878 16-17 21.692396131889208 27.799049792251836 27.73151705974342 22.777037016115536 18-19 21.283820432242788 27.677755925759595 29.31396872307335 21.724454918924266 20-21 22.536789218940907 26.67561762678712 28.885886499858426 21.901706654413548 22-23 22.111161931580657 26.6793132351257 28.849436279210938 22.3600885540827 24-25 22.05243804196724 26.492510206137776 29.261112581080358 22.193939170814623 26-27 21.980758670345597 26.356386431863953 29.398152701953023 22.264702195837422 28-29 21.504114393632808 27.656861257250775 29.047338009802598 21.791686339313816 30-31 22.417176210645298 26.626468033275934 29.113422986093994 21.84293276998477 32-33 22.329309265848586 26.79466518860032 28.937378884122683 21.93864666142841 34-35 22.546170322812777 26.78535086634359 29.119278253145488 21.549200557698146 36-37 22.203162241897857 26.580702781955935 29.29312352174461 21.923011454401596 38-39 21.962401800782782 27.0496468932112 29.181765949959015 21.806185356047003 40-41 21.97031279969817 26.5575873651995 29.38175671165813 22.090343123444203 42-43 21.57127418045059 26.866252386551086 28.82567283281067 22.73680060018765 44-45 21.357148628945843 26.91147610097142 29.121337853742514 22.610037416340226 46-47 21.571034766740237 26.7532514986512 28.9141340146566 22.761579719951964 48-49 21.5988056483256 26.734129998726843 29.013125333571665 22.653939019375894 50-51 21.005407543015888 27.010845300479875 29.163097731372755 22.820649425131478 52-53 21.12394701115599 27.3205105502666 28.446692132342815 23.108850306234594 54-55 20.722905924194993 26.873328727643525 28.935574119470992 23.46819122869049 56-57 21.34350466243687 26.55612580787632 28.528206130592636 23.57216339909417 58-59 21.298138782696093 26.769733169870435 28.553982946570457 23.378145100863016 60-61 21.256342698958047 27.03745547288325 28.53958083032973 23.166620997828975 62-63 21.066954149536247 26.780774545625214 28.364998323609896 23.787272981228647 64-65 20.579461156330662 26.93567623618408 28.17231708849356 24.312545518991698 66-67 20.879169851458542 26.53980264670087 27.800242168364232 24.780785333476356 68-69 20.606687575033853 26.686652729942246 27.928698034466326 24.777961660557573 70-71 20.88056861017539 26.464112577099346 27.83804951537755 24.81726929734771 72-73 20.772410027641197 26.484085471087514 27.922333055979504 24.82117144529178 74-75 20.62249710345355 27.03552052166088 27.444094158597355 24.897888216288212 76-77 20.590452386678436 26.675157294717796 27.51766073592409 25.21672958267968 78-79 20.557485560099497 26.707632418454967 27.601093356943096 25.133788664502436 80-81 20.691487268843797 26.61285200382216 27.435276263279185 25.26038446405486 82-83 20.7052196208967 26.324231739394804 27.85489174480904 25.11565689489946 84-85 20.673691444021376 26.665474501441565 27.378524268153946 25.282309786383113 86-87 20.266888007219436 26.85701335620969 27.438285364263653 25.437813272307224 88-89 20.323853817402934 26.46753510792526 27.317838730545454 25.89077234412635 90-91 20.88807697649221 25.86986798551827 27.331746055843155 25.91030898214636 92-93 20.839555786751664 26.10220176814425 27.402286339631615 25.655956105472466 94-95 20.212334358388322 25.768541805759693 27.87162131303066 26.147502522821327 96-97 20.008023826972003 25.789999163891935 28.152517352755396 26.049459656380662 98-99 20.147775039901596 26.032213052866872 27.610792629210046 26.20921927802149 100 20.095760891227286 25.569237333705118 27.686669509398303 26.648332265669296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1043.0 1 760.5 2 1120.5 3 1810.0 4 1382.0 5 1027.5 6 1498.5 7 2655.5 8 3883.0 9 4394.5 10 3830.5 11 3027.5 12 3064.5 13 3403.0 14 3783.0 15 4179.0 16 4580.5 17 5032.5 18 5583.0 19 6181.5 20 6849.5 21 7909.0 22 9385.0 23 11082.0 24 13299.5 25 16192.5 26 19534.0 27 23262.0 28 27030.5 29 31727.0 30 37971.5 31 44015.5 32 50875.0 33 58103.0 34 64187.0 35 70475.5 36 77758.5 37 86141.0 38 91501.0 39 93459.5 40 94751.0 41 96489.0 42 100767.5 43 106327.5 44 112889.0 45 122554.5 46 131950.5 47 145906.0 48 166570.0 49 170996.0 50 162081.5 51 156967.0 52 149927.5 53 140714.0 54 131476.5 55 118360.0 56 103945.0 57 89782.0 58 76288.5 59 65914.5 60 52887.5 61 39566.5 62 30926.5 63 23977.5 64 17395.0 65 12189.5 66 8669.0 67 6504.0 68 5023.5 69 3648.0 70 2696.5 71 2025.0 72 1557.0 73 1215.5 74 939.5 75 700.0 76 475.0 77 271.0 78 157.5 79 104.5 80 67.0 81 47.5 82 31.0 83 20.5 84 13.0 85 10.5 86 8.0 87 4.0 88 3.5 89 3.5 90 2.5 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05373385665763046 2 0.0 3 0.0 4 0.013742602461078085 5 0.003793970004592109 6 0.0 7 1.405174075774855E-4 8 4.4965570424795366E-4 9 0.0017705193354763174 10-11 8.15000963949416E-4 12-13 2.669830743972225E-4 14-15 0.0 16-17 9.836218530423987E-5 18-19 0.0 20-21 1.4051740757748552E-5 22-23 1.4051740757748552E-5 24-25 7.025870378874276E-5 26-27 0.0 28-29 0.00106793229758889 30-31 8.712079269804102E-4 32-33 0.01339130894213437 34-35 0.02242657824936669 36-37 0.0305344326665876 38-39 0.02006588580206493 40-41 2.9508655591271957E-4 42-43 0.0016159501871410833 44-45 0.009569235456026764 46-47 0.013925275090928814 48-49 0.005536385858552929 50-51 0.010609064272100156 52-53 0.015400707870492412 54-55 0.010117253345578956 56-57 0.0029930207814004416 58-59 0.007981388750401178 60-61 0.0014894845203213465 62-63 0.001039828816073393 64-65 0.01958812661630148 66-67 0.04669393453799844 68-69 0.05526549640022505 70-71 0.04908273046681569 72-73 0.04073599645671305 74-75 0.028792016812626785 76-77 0.02873580984959579 78-79 0.01202829008863276 80-81 0.0021920715582087738 82-83 0.027794343218826636 84-85 0.048450402132717 86-87 0.049672903578641125 88-89 0.06004308825785956 90-91 0.0349607310052784 92-93 0.02620649651320105 94-95 0.007236646490240505 96-97 0.003526986930194886 98-99 0.021808301656025753 100 0.018745022170836567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3558278.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.08730975226433 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9974773263693 50.11558656884348 2 14.952760192014628 19.464698685379673 3 4.1662012747450134 8.135004985788216 4 1.4990596856192573 3.9027904838013034 5 0.7117909375923962 2.3164278616965475 6 0.4258116126386823 1.6628959396755065 7 0.2671013909433584 1.2169437677313715 8 0.1792167114702304 0.9331786889796058 9 0.1285820053989295 0.7532151132570706 >10 0.6360343073750266 6.988658529261055 >50 0.02439013636294623 1.0814066944962837 >100 0.01042782685810755 1.2050418869828734 >500 5.255216145844354E-4 0.2459635216095451 >1k 4.2997222903871555E-4 0.6039943659869461 >5k 4.777469211541285E-5 0.24804690370046686 >10k+ 1.4332407634623853E-4 1.1261460028100927 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12865 0.3615512896968702 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12047 0.3385626418171936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11304 0.31768175505117924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7977 0.2241814720491204 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.620696303099421E-5 10-11 0.0 0.0 0.0 7.025870378874276E-5 5.620696303099421E-5 12-13 0.0 0.0 0.0 8.43104445464913E-5 5.620696303099421E-5 14-15 0.0 0.0 0.0 1.1241392606198841E-4 5.620696303099421E-5 16-17 0.0 0.0 0.0 1.2646566681973696E-4 8.43104445464913E-5 18-19 0.0 0.0 0.0 1.686208890929826E-4 1.1241392606198841E-4 20-21 0.0 0.0 0.0 3.091382966704681E-4 1.1241392606198841E-4 22-23 0.0 0.0 0.0 0.0010538805568311414 1.2646566681973696E-4 24-25 0.0 2.8103481515497104E-5 0.0 0.002599572040183482 1.405174075774855E-4 26-27 0.0 2.8103481515497104E-5 0.0 0.00621086941492486 1.405174075774855E-4 28-29 0.0 2.8103481515497104E-5 0.0 0.031124605778413042 1.405174075774855E-4 30-31 0.0 2.8103481515497104E-5 0.0 0.11277927132168987 1.405174075774855E-4 32-33 0.0 2.8103481515497104E-5 0.0 0.262093068613526 1.686208890929826E-4 34-35 0.0 2.8103481515497104E-5 0.0 0.48980152759284123 1.9672437060847973E-4 36-37 0.0 2.8103481515497104E-5 0.0 0.8505518680665198 1.9672437060847973E-4 38-39 0.0 2.8103481515497104E-5 0.0 1.3912347489431687 2.2482785212397683E-4 40-41 0.0 2.8103481515497104E-5 0.0 1.9732719028698713 2.529313336394739E-4 42-43 0.0 2.8103481515497104E-5 0.0 2.5127041788190807 2.529313336394739E-4 44-45 0.0 2.8103481515497104E-5 0.0 3.1454681168812555 2.669830743972225E-4 46-47 0.0 2.8103481515497104E-5 0.0 3.904135652132857 2.9508655591271957E-4 48-49 0.0 2.8103481515497104E-5 0.0 4.666147501684804 3.372417781859652E-4 50-51 0.0 2.8103481515497104E-5 0.0 5.44244997158738 3.372417781859652E-4 52-53 0.0 2.8103481515497104E-5 0.0 6.171636954729226 3.9344874121695946E-4 54-55 0.0 2.8103481515497104E-5 0.0 6.966136428913087 4.4965570424795366E-4 56-57 0.0 2.8103481515497104E-5 0.0 7.860290848550901 4.4965570424795366E-4 58-59 0.0 2.8103481515497104E-5 0.0 8.809247057143933 4.4965570424795366E-4 60-61 0.0 2.8103481515497104E-5 0.0 9.734202892522731 4.4965570424795366E-4 62-63 0.0 2.8103481515497104E-5 0.0 10.668629601172253 4.4965570424795366E-4 64-65 0.0 2.8103481515497104E-5 0.0 11.533879590071376 4.4965570424795366E-4 66-67 0.0 5.620696303099421E-5 0.0 12.482006745959703 4.4965570424795366E-4 68-69 0.0 5.620696303099421E-5 0.0 13.510945462945841 4.4965570424795366E-4 70-71 0.0 5.620696303099421E-5 0.0 14.556451182285365 4.4965570424795366E-4 72-73 0.0 5.620696303099421E-5 0.0 15.50838635991904 5.901731118254391E-4 74-75 0.0 5.620696303099421E-5 0.0 16.42389942550863 5.901731118254391E-4 76-77 0.0 5.620696303099421E-5 0.0 17.38215788648329 5.901731118254391E-4 78-79 2.8103481515497104E-5 5.620696303099421E-5 0.0 18.42245041000169 5.901731118254391E-4 80-81 2.8103481515497104E-5 5.620696303099421E-5 0.0 19.447553001760966 5.901731118254391E-4 82-83 2.8103481515497104E-5 5.620696303099421E-5 0.0 20.435825980994178 6.182765933409362E-4 84-85 2.8103481515497104E-5 5.620696303099421E-5 0.0 21.36423854459938 6.463800748564333E-4 86-87 2.8103481515497104E-5 5.620696303099421E-5 0.0 22.288168602902864 6.744835563719304E-4 88 2.8103481515497104E-5 5.620696303099421E-5 0.0 23.00767393666262 6.744835563719304E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3705 0.0 53.899902 2 ACCTGGG 4120 0.0 46.98869 3 CCTGGGG 4050 0.0 35.27102 4 GTACCTG 6540 0.0 30.987024 1 GTACATG 10675 0.0 29.643366 1 CTGGGGG 2935 0.0 29.294598 5 TACATGG 11010 0.0 28.167213 2 TGGGGGG 6245 0.0 27.835667 6 TAACGCA 390 0.0 27.71167 4 ACATGGG 11065 0.0 26.965944 3 CATGGGG 7750 0.0 26.37467 4 ATGGGGG 5890 0.0 26.08417 5 TCGTGAT 650 0.0 23.855463 2 TATCACG 275 6.002665E-11 22.212523 2 ATAACGC 450 0.0 21.928055 3 CTAACGC 515 0.0 21.897642 3 GAGTACA 8015 0.0 21.529942 1 CGTTAAC 410 0.0 20.642818 1 GAGTACT 11500 0.0 20.630976 12-13 AGTACAT 8035 0.0 19.882927 2 >>END_MODULE