FastQCFastQC Report
Mon 6 Jun 2016
ERR1211392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18601463
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780410.419542269336557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT596480.320662950005599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376660.2024894493513763No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC303360.1630839466766673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA427250.048.027151
GTATCAA761050.040.6445431
TACCTGG71050.040.1465342
ACCTGGG76500.036.8085333
GTACTAG172300.035.2491261
CAACGCA894650.033.4496775
TAGGCAT202800.033.2398955
ATCAACG902450.033.1557123
TATTCTC100700.032.117185
TATCAAC963950.031.9493452
GCATCGT181050.031.4160278
AACGCAG1000300.030.7116476
GGGCACC153050.030.36956894
CTAGGCA223900.030.2122294
CTGTGCG194650.029.4858749
TCAACGC1058100.028.5445044
TAGTACT204800.028.3473914
CCTGGGG81600.028.343344
ACTGTGC212400.028.2839958
GTACATG1042350.028.283511
Produced by FastQC (version 0.11.5)