FastQCFastQC Report
Mon 6 Jun 2016
ERR1211392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18601463
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT722160.3882275281250727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT593930.3192920900899031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378400.2034248596467923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA420850.048.9883961
GTATCAA755950.040.8809971
TACCTGG69500.037.3234832
GTACTAG175550.035.406381
ACCTGGG71400.035.146263
TAGGCAT204050.033.940725
GGGCACC155700.033.62704594
CAACGCA912650.032.8507045
ATCAACG922950.032.474493
TATTCTC108000.032.3675165
TATCAAC979550.031.465812
CTAGGCA223650.031.224324
AACGCAG1015000.030.3615256
CTGTGCG206250.030.2522229
ACTGTGC223050.029.3216888
TAGTACT216550.029.0579814
GCTATTG258600.028.851878
AGGGCTA262500.028.6206385
CTATTGA263450.028.3029639
GCATCGT207600.028.2222168
Produced by FastQC (version 0.11.5)