FastQCFastQC Report
Mon 6 Jun 2016
ERR1211391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648571
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134270.36800709099535134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109300.29956933824228715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.1660101996096554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.053.589361
GTATCAA102700.039.214311
TACCTGG26350.032.5069242
ATCAACG123250.032.494323
CAACGCA123050.032.4707345
TATCAAC128100.031.412472
TCAACGC129500.031.0349564
GTGGTAT24050.030.5533241
AACGCAG133050.030.4895296
ACGCAGA145350.027.424687
CGCAGAG147700.026.8917528
TGGTATC26200.025.6873822
GTACATG95100.024.5173551
GCAGAGT161400.023.9101629
GTACTAG12250.023.8399071
ACCTGGG35100.023.8378833
TACATGG94200.023.7816052
ACATGGG95650.022.2130553
CAGAGTA157850.021.84230410-11
GAGTACT112150.021.75050212-13
Produced by FastQC (version 0.11.5)