FastQCFastQC Report
Mon 6 Jun 2016
ERR1211391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648571
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119680.32801883257856296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103990.28501569518586867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61890.1696280543807425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.053.398451
GTATCAA101200.038.9724961
TACCTGG28650.033.950322
ATCAACG124200.031.251763
CAACGCA125100.031.136635
TATCAAC128500.030.5704152
TCAACGC130750.029.7944134
AACGCAG134600.029.3908146
GTGGTAT23200.028.3672241
ACCTGGG34150.027.3828333
ATAACGC3300.027.0555063
ACGCAGA149850.026.1175757
CGCAGAG151700.025.3964428
TAACGCA3800.024.7325214
TGGTATC26900.023.4072672
GCAGAGT165900.022.85459
GTACATG98950.022.8510441
TACATGG100650.021.8022482
GTACTAG13350.021.4795591
GTACCTG47100.021.0590291
Produced by FastQC (version 0.11.5)