FastQCFastQC Report
Mon 6 Jun 2016
ERR1211390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206307
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186390.4431202953089254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148060.3519952300200627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.20875794372593345No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50320.11962987960698065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44180.10503275200787769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73950.056.1276441
TACCTGG34350.044.3943632
GTATCAA135400.043.702881
TATCAAC169050.034.7184942
ATCAACG167650.034.573793
ACCTGGG43050.034.2881433
CAACGCA169300.034.208665
TCAACGC177000.032.826724
AACGCAG182000.032.106846
ACGCAGA196950.029.2400387
GTACATG135500.028.9283141
CGCAGAG201250.028.4717378
GTGGTAT28300.027.9680351
TACATGG138450.027.6380372
ACATGGG139450.026.429263
GCAGAGT214500.025.923489
CCTGGGG41200.025.7865684
GTACCTG61800.025.4622691
CATGGGG112050.025.3400464
ATGGGGG88250.025.3023725
Produced by FastQC (version 0.11.5)