##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211390_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4206307 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40223526242854 31.0 31.0 34.0 30.0 34.0 2 31.444154694367292 31.0 31.0 34.0 28.0 34.0 3 31.503097848064822 31.0 31.0 34.0 28.0 34.0 4 35.141637307975856 37.0 35.0 37.0 32.0 37.0 5 35.19902993290789 37.0 35.0 37.0 32.0 37.0 6 35.266891360996716 37.0 35.0 37.0 32.0 37.0 7 35.20213907353885 37.0 35.0 37.0 32.0 37.0 8 35.205894148952986 37.0 35.0 37.0 32.0 37.0 9 36.781910592831196 39.0 37.0 39.0 32.0 39.0 10-11 36.711460551975875 39.0 37.0 39.0 32.0 39.0 12-13 36.61853010253412 39.0 37.0 39.0 32.0 39.0 14-15 37.80114040653714 40.0 37.0 41.0 32.0 41.0 16-17 37.78718731181533 40.0 37.0 41.0 32.0 41.0 18-19 37.79179705618253 40.0 37.0 41.0 32.0 41.0 20-21 37.777524560142666 40.0 37.0 41.0 32.0 41.0 22-23 37.651645731041505 40.0 37.0 41.0 32.0 41.0 24-25 37.55543069015172 40.0 37.0 41.0 32.0 41.0 26-27 37.413384472412496 40.0 37.0 41.0 31.5 41.0 28-29 37.27582782236294 40.0 36.0 41.0 31.0 41.0 30-31 37.10569711150423 39.0 36.0 41.0 31.0 41.0 32-33 36.88454920194841 39.0 35.5 41.0 30.0 41.0 34-35 36.752700052563924 39.0 35.0 41.0 30.0 41.0 36-37 36.6141765211146 39.0 35.0 41.0 30.0 41.0 38-39 36.45164879786473 39.0 35.0 41.0 30.0 41.0 40-41 36.27619679210291 39.0 35.0 40.0 29.0 41.0 42-43 36.074973010766925 39.0 35.0 40.0 28.5 41.0 44-45 35.93133038078295 38.5 35.0 40.0 27.5 41.0 46-47 35.69718615403012 38.0 35.0 40.0 27.0 41.0 48-49 35.43613554597893 38.0 34.0 40.0 26.0 41.0 50-51 34.76315197630606 37.5 33.5 39.5 25.0 40.5 52-53 35.05768242783991 38.0 34.0 39.5 26.0 40.5 54-55 35.45798987092478 38.0 34.0 40.0 26.0 41.0 56-57 35.37558064592052 38.0 34.0 40.0 26.0 41.0 58-59 35.17062484977915 38.0 34.0 40.0 26.0 41.0 60-61 34.94071438437565 38.0 34.0 40.0 26.0 41.0 62-63 34.650463696539504 37.0 33.5 40.0 25.5 41.0 64-65 34.43132051464622 37.0 33.0 40.0 25.0 41.0 66-67 34.22240162213552 36.0 33.0 39.5 25.0 41.0 68-69 33.88155179828767 36.0 33.0 39.0 24.0 41.0 70-71 33.50393956028412 35.0 32.5 39.0 24.0 41.0 72-73 33.11703924606549 35.0 32.0 38.0 23.0 40.0 74-75 32.73767547161917 35.0 32.0 37.0 22.5 39.5 76-77 32.34622591741402 35.0 31.5 37.0 21.0 39.0 78-79 31.937910024161337 35.0 31.0 36.0 20.0 39.0 80-81 31.540751067385237 35.0 31.0 36.0 19.5 38.0 82-83 31.165428367449167 35.0 30.5 35.0 18.5 37.0 84-85 30.829485698499894 34.0 30.0 35.0 18.0 37.0 86-87 30.485025700691843 34.0 29.5 35.0 17.0 36.0 88-89 30.159160280027113 34.0 29.0 35.0 15.0 36.0 90-91 29.848320866736543 34.0 29.0 35.0 11.5 36.0 92-93 29.55055515443832 34.0 29.0 35.0 10.0 35.0 94-95 29.25401438839343 34.0 27.5 35.0 10.0 35.0 96-97 28.94892396108986 34.0 27.0 35.0 10.0 35.0 98-99 28.620189872018376 34.0 25.5 35.0 9.0 35.0 100 27.35770070991014 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3187750869066903 1101 2 -0.2551611632991424 1101 3 -0.3588745844326091 1101 4 -0.22883190994586045 1101 5 -0.2212537721567216 1101 6 -0.2393363236107362 1101 7 -0.25267769650081107 1101 8 -0.24578152553246468 1101 9 -0.44992664286348116 1101 10-11 -0.35425745331799874 1101 12-13 -0.395575022233551 1101 14-15 -0.6241716759376956 1101 16-17 -0.610961831683305 1101 18-19 -0.6645893803795886 1101 20-21 -0.6244359727801552 1101 22-23 -0.6064171883237179 1101 24-25 -0.6985686834783706 1101 26-27 -0.7692922107443678 1101 28-29 -0.7875559812318826 1101 30-31 -0.6554126182598239 1101 32-33 -0.6161457358469491 1101 34-35 -0.7625977096363599 1101 36-37 -0.9062216831963568 1101 38-39 -0.8730373215610356 1101 40-41 -0.8750259243083249 1101 42-43 -0.8905899385274694 1101 44-45 -0.9262873405159127 1101 46-47 -0.9090458308869316 1101 48-49 -0.8824857288027914 1101 50-51 -0.8823092083904953 1101 52-53 -0.577302282430324 1101 54-55 -0.551449852434402 1101 56-57 -0.6230364346131694 1101 58-59 -0.5775746559426267 1101 60-61 -0.6157489281707456 1101 62-63 -0.6861225855257587 1101 64-65 -0.6073264439449915 1101 66-67 -0.7702157905707878 1101 68-69 -0.7934370588685269 1101 70-71 -0.6682860659804746 1101 72-73 -0.8671721271956585 1101 74-75 -0.8571641063980913 1101 76-77 -0.7628731773691015 1101 78-79 -0.9013476457687837 1101 80-81 -1.0635642513824948 1101 82-83 -1.0728370264365061 1101 84-85 -1.0991819003860925 1101 86-87 -1.151708459737268 1101 88-89 -1.0698755449138204 1101 90-91 -1.3212237984040485 1101 92-93 -1.4109967269486958 1101 94-95 -1.454337085216757 1101 96-97 -1.5100491175775659 1101 98-99 -1.5787952296451415 1101 100 -1.5108775078100258 1102 1 -0.47205526156656674 1102 2 -0.45006769758305865 1102 3 -0.5646568099538456 1102 4 -0.4629044207704851 1102 5 -0.32604384266991815 1102 6 -0.3480355538139932 1102 7 -0.3020664281371168 1102 8 -0.24722423033181684 1102 9 -0.49431047043109544 1102 10-11 -0.363916289304818 1102 12-13 -0.4732596772800832 1102 14-15 -0.6708527067657784 1102 16-17 -0.679460059040828 1102 18-19 -0.7286043369759199 1102 20-21 -0.7971169814895163 1102 22-23 -0.7702504894547886 1102 24-25 -0.7541051200690845 1102 26-27 -0.7939750834985873 1102 28-29 -0.7588510782257529 1102 30-31 -0.8064739810292778 1102 32-33 -0.8722097908124269 1102 34-35 -0.7870934406136598 1102 36-37 -0.8799216496062527 1102 38-39 -0.9939650633669643 1102 40-41 -1.1366924142420274 1102 42-43 -0.9740284984508918 1102 44-45 -0.8797345766183611 1102 46-47 -0.968476572924672 1102 48-49 -1.0835775915617276 1102 50-51 -1.069978798849256 1102 52-53 -0.7752006997920518 1102 54-55 -0.7786739066799342 1102 56-57 -0.7831447903525515 1102 58-59 -0.8094693395765233 1102 60-61 -0.8431936275588185 1102 62-63 -0.8694096321704663 1102 64-65 -0.9110199243635222 1102 66-67 -1.0001271616998153 1102 68-69 -0.8417563934331369 1102 70-71 -0.9417436805365114 1102 72-73 -0.8190894846930519 1102 74-75 -0.9406052685246706 1102 76-77 -0.8716262948626934 1102 78-79 -1.0450587604506936 1102 80-81 -1.0030377649661055 1102 82-83 -1.0034203297902913 1102 84-85 -1.093654387183708 1102 86-87 -1.1354542314282021 1102 88-89 -1.142988779567272 1102 90-91 -1.2235473923415228 1102 92-93 -1.087625169419514 1102 94-95 -1.1205517275103176 1102 96-97 -1.2440452971096363 1102 98-99 -1.499084759442212 1102 100 -1.4457569401480797 1103 1 -0.41352097333065174 1103 2 -0.4649252139733342 1103 3 -0.6143244987417056 1103 4 -0.4112352162799269 1103 5 -0.29182400476154413 1103 6 -0.26768127684803034 1103 7 -0.3704171194513606 1103 8 -0.3489399433494498 1103 9 -0.4933298753380555 1103 10-11 -0.5017590041118183 1103 12-13 -0.5925793144860236 1103 14-15 -0.6061573363822603 1103 16-17 -0.7113080362218156 1103 18-19 -0.7068513338019287 1103 20-21 -0.7090716186026 1103 22-23 -0.7017723243095801 1103 24-25 -0.6221341818426112 1103 26-27 -0.8178702473250539 1103 28-29 -0.8596528992159094 1103 30-31 -0.7974067780261791 1103 32-33 -0.8832525203936754 1103 34-35 -0.8308253687263303 1103 36-37 -0.8755074179899083 1103 38-39 -0.8992373174552952 1103 40-41 -0.8354834397170947 1103 42-43 -0.9464014940838013 1103 44-45 -0.8845748038847816 1103 46-47 -1.0724812357126083 1103 48-49 -1.0278763297685458 1103 50-51 -1.0607376170192424 1103 52-53 -0.7579609411490935 1103 54-55 -0.664909515467059 1103 56-57 -0.7123021798551648 1103 58-59 -0.7808496636481976 1103 60-61 -0.7651652006826453 1103 62-63 -0.8165888552213474 1103 64-65 -0.8229416866465087 1103 66-67 -0.7604407303745617 1103 68-69 -0.7759061666391815 1103 70-71 -0.890037893528266 1103 72-73 -0.894931647089912 1103 74-75 -0.9933878739471211 1103 76-77 -0.9476509461123008 1103 78-79 -0.8991290846205295 1103 80-81 -0.9844312114269904 1103 82-83 -0.9296175368210022 1103 84-85 -1.0292284420994342 1103 86-87 -1.1928898435487092 1103 88-89 -1.1153032290473455 1103 90-91 -0.9407403423112548 1103 92-93 -1.0145337934231975 1103 94-95 -1.133316554394149 1103 96-97 -1.2057813607417458 1103 98-99 -1.2432803744977967 1103 100 -1.2409213245388138 1104 1 -0.4878829586351543 1104 2 -0.44629392597045836 1104 3 -0.4564007373889254 1104 4 -0.36146279641905465 1104 5 -0.4518562744223331 1104 6 -0.256715816715122 1104 7 -0.2854698601344907 1104 8 -0.34449052083230924 1104 9 -0.4432686091420237 1104 10-11 -0.35690728788900117 1104 12-13 -0.4907125838488753 1104 14-15 -0.7158869118690703 1104 16-17 -0.7715369032308033 1104 18-19 -0.6906468445843572 1104 20-21 -0.7686843267223225 1104 22-23 -0.7235136410851908 1104 24-25 -0.6983118426209245 1104 26-27 -0.7022259661328007 1104 28-29 -0.7724594225271417 1104 30-31 -0.7650814811019373 1104 32-33 -0.6850251137022312 1104 34-35 -0.7803494716591288 1104 36-37 -0.839907456875757 1104 38-39 -0.9060196462141832 1104 40-41 -0.8493510012245764 1104 42-43 -0.8216599870752503 1104 44-45 -0.7224254934092329 1104 46-47 -0.7696172158948045 1104 48-49 -0.8278912266860772 1104 50-51 -0.8677066817865651 1104 52-53 -0.6672069263249085 1104 54-55 -0.5589281233256287 1104 56-57 -0.624807512671886 1104 58-59 -0.6855921637845768 1104 60-61 -0.5727795887270588 1104 62-63 -0.6765117409431056 1104 64-65 -0.7088965161152672 1104 66-67 -0.5726985855261688 1104 68-69 -0.5608903185569645 1104 70-71 -0.5062907103045688 1104 72-73 -0.6807324368363581 1104 74-75 -0.6393656362762385 1104 76-77 -0.6986535855943075 1104 78-79 -0.5533732024362656 1104 80-81 -0.6453894678861651 1104 82-83 -0.6433517604258299 1104 84-85 -0.6176656015859727 1104 86-87 -0.7356550471469099 1104 88-89 -0.6954442741815079 1104 90-91 -0.7464448476147005 1104 92-93 -0.8219150761806802 1104 94-95 -0.834329125154504 1104 96-97 -0.8021454987557419 1104 98-99 -0.9446188240713802 1104 100 -0.8801344875390669 1105 1 -0.44114924000494327 1105 2 -0.4390861734696401 1105 3 -0.4509667124628791 1105 4 -0.3691394479526693 1105 5 -0.4774467462614709 1105 6 -0.3769550145761542 1105 7 -0.3958024789155772 1105 8 -0.41808358217567587 1105 9 -0.5737113646726755 1105 10-11 -0.49818037567955287 1105 12-13 -0.629922384763475 1105 14-15 -0.7696962851565914 1105 16-17 -0.8519352120471311 1105 18-19 -0.7595113690666295 1105 20-21 -0.7534545634355894 1105 22-23 -0.7721825862211062 1105 24-25 -0.7597712960367602 1105 26-27 -0.6537536493961298 1105 28-29 -0.6781892527600135 1105 30-31 -0.7104707211321397 1105 32-33 -0.6914931458860636 1105 34-35 -0.8476201604313687 1105 36-37 -0.8165827667259506 1105 38-39 -0.845505134085883 1105 40-41 -0.7565793896999011 1105 42-43 -0.8313583084526073 1105 44-45 -0.7439174812678289 1105 46-47 -0.8193011542049788 1105 48-49 -0.9634543265269144 1105 50-51 -0.9347026153263016 1105 52-53 -0.7242864114055863 1105 54-55 -0.7400053142932848 1105 56-57 -0.7511229007593414 1105 58-59 -0.6956493716751027 1105 60-61 -0.73988229100447 1105 62-63 -0.7819925591491383 1105 64-65 -0.7547568637106608 1105 66-67 -0.7136551285104247 1105 68-69 -0.7426578829460411 1105 70-71 -0.7636217955359754 1105 72-73 -0.6735992913203006 1105 74-75 -0.6851962722093425 1105 76-77 -0.8000430975500592 1105 78-79 -0.8382775039671913 1105 80-81 -0.9068173806988291 1105 82-83 -0.8489742352796199 1105 84-85 -0.8257692245453221 1105 86-87 -0.9089856382191961 1105 88-89 -0.9810211428414668 1105 90-91 -0.9962238218342243 1105 92-93 -1.0643310104657964 1105 94-95 -1.1273551320037178 1105 96-97 -1.2790915608734785 1105 98-99 -1.2315179134658685 1105 100 -1.277005273210662 1106 1 -0.38531859590398554 1106 2 -0.4266360664794071 1106 3 -0.4104782838261478 1106 4 -0.33841870642621785 1106 5 -0.4482556142223757 1106 6 -0.42313017335838055 1106 7 -0.42204196127496374 1106 8 -0.420326255507689 1106 9 -0.5237311549704557 1106 10-11 -0.6010077654516763 1106 12-13 -0.496458217464955 1106 14-15 -0.6821186312299758 1106 16-17 -0.7129806847695619 1106 18-19 -0.7473780430907908 1106 20-21 -0.8031452761656155 1106 22-23 -0.6745418213414212 1106 24-25 -0.6695647343778859 1106 26-27 -0.7079851284364693 1106 28-29 -0.7934072423791392 1106 30-31 -0.7324891816309034 1106 32-33 -0.8032597552596528 1106 34-35 -0.7915190943440322 1106 36-37 -0.7904962930027679 1106 38-39 -0.932726290152651 1106 40-41 -0.9795896472735208 1106 42-43 -0.7649101866233963 1106 44-45 -0.8897625065455941 1106 46-47 -0.8580748778670184 1106 48-49 -0.7799962904738322 1106 50-51 -0.7393089519003411 1106 52-53 -0.7533335954267457 1106 54-55 -0.665552783417354 1106 56-57 -0.8400844449066867 1106 58-59 -0.765333292347691 1106 60-61 -0.6561700807654347 1106 62-63 -0.8099174527550019 1106 64-65 -0.8748079437976699 1106 66-67 -0.6585976344594329 1106 68-69 -0.7232004096050346 1106 70-71 -0.6789499091356888 1106 72-73 -0.6406022908618212 1106 74-75 -0.49110474906490964 1106 76-77 -0.5087500451367681 1106 78-79 -0.6462615095915858 1106 80-81 -0.625767459417208 1106 82-83 -0.600052857602396 1106 84-85 -0.7233134904342755 1106 86-87 -0.6312656644609262 1106 88-89 -0.7295944020276082 1106 90-91 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42-43 1.3884780328461872 2311 44-45 1.367901615703076 2311 46-47 1.3712537039426778 2311 48-49 1.4429761914997314 2311 50-51 1.4380818015763666 2311 52-53 1.3258717216156555 2311 54-55 1.3363489712316152 2311 56-57 1.3736161112422849 2311 58-59 1.226102825478435 2311 60-61 1.2207576490182106 2311 62-63 1.2068225064760085 2311 64-65 1.1615734856988453 2311 66-67 1.2564789271198507 2311 68-69 1.2571466681662926 2311 70-71 1.1959952776041831 2311 72-73 1.2448533515204474 2311 74-75 1.3152776382321605 2311 76-77 1.2747031694293014 2311 78-79 1.248171743161567 2311 80-81 1.2559849303992898 2311 82-83 1.4076368233459071 2311 84-85 1.3681289780259753 2311 86-87 1.3378946151335498 2311 88-89 1.2856146379439082 2311 90-91 1.3573538116324038 2311 92-93 1.4217413442875326 2311 94-95 1.4485341923018602 2311 96-97 1.5056747863546427 2311 98-99 1.4947612570150604 2311 100 1.50812422123742 2312 1 0.6161019635107614 2312 2 0.656823424610895 2312 3 0.6265848307078983 2312 4 0.5510945742207767 2312 5 0.5692374224567089 2312 6 0.4425126544210727 2312 7 0.5824858752296223 2312 8 0.6386401737349061 2312 9 0.803743655425869 2312 10-11 0.8352636383705985 2312 12-13 0.8904519976429413 2312 14-15 1.1861140485579327 2312 16-17 1.1359416783969891 2312 18-19 1.1384557936151438 2312 20-21 1.1894440305225302 2312 22-23 1.150537512277154 2312 24-25 1.1775990756492902 2312 26-27 1.1717226637713267 2312 28-29 1.265765359889393 2312 30-31 1.0918766213322755 2312 32-33 1.1831306752078135 2312 34-35 1.2427029013605164 2312 36-37 1.2542568866341384 2312 38-39 1.2696661454156626 2312 40-41 1.3750803919895418 2312 42-43 1.4737088211453226 2312 44-45 1.3443747547718985 2312 46-47 1.2968766305728607 2312 48-49 1.354602972504793 2312 50-51 1.3740641373811755 2312 52-53 1.2188129111674328 2312 54-55 1.191488769990471 2312 56-57 1.1085574453413045 2312 58-59 1.1604152646508652 2312 60-61 1.1260552560747357 2312 62-63 1.155621554426446 2312 64-65 1.1063933968513524 2312 66-67 1.085176066000308 2312 68-69 1.1600870434135189 2312 70-71 1.1734790209458552 2312 72-73 1.0979504395150457 2312 74-75 1.1217413195951167 2312 76-77 1.0864145367283982 2312 78-79 1.1257081873781054 2312 80-81 0.8653913252735528 2312 82-83 1.0593753635627472 2312 84-85 1.1054881262307141 2312 86-87 1.134476841579545 2312 88-89 1.1141239798811853 2312 90-91 1.196914394582894 2312 92-93 1.2094767567900178 2312 94-95 1.2808365910010657 2312 96-97 1.2379880260963674 2312 98-99 1.0953510882152848 2312 100 1.2497779266174582 2313 1 0.5205868072696234 2313 2 0.5739381739445335 2313 3 0.607818526051858 2313 4 0.5440711664058853 2313 5 0.5042385566936503 2313 6 0.5456156785803188 2313 7 0.5641750375956391 2313 8 0.5597974144199824 2313 9 0.7260085493747113 2313 10-11 0.604951953603404 2313 12-13 0.7250199951476191 2313 14-15 0.9466704272229407 2313 16-17 0.9389637109495865 2313 18-19 0.9379971365516795 2313 20-21 0.9496078568708484 2313 22-23 1.0092315270505878 2313 24-25 0.9717838074022609 2313 26-27 1.0392756893483721 2313 28-29 0.9635115956405471 2313 30-31 1.0219531291585113 2313 32-33 1.020060632532143 2313 34-35 1.0165945391986568 2313 36-37 1.1417972938251992 2313 38-39 1.1759174745995793 2313 40-41 1.1113171355952787 2313 42-43 1.156197354718735 2313 44-45 1.1563129530365188 2313 46-47 1.1102662976743218 2313 48-49 1.1707409488843012 2313 50-51 1.163620207675514 2313 52-53 1.0652709018666968 2313 54-55 1.0384819787467876 2313 56-57 1.0505904055579407 2313 58-59 1.0538696541478316 2313 60-61 1.0178459503695194 2313 62-63 1.0510262997902515 2313 64-65 1.0052318673328315 2313 66-67 0.9893224109217655 2313 68-69 1.0395996228101083 2313 70-71 1.0256237035012887 2313 72-73 1.0128795674286124 2313 74-75 1.0098063113718894 2313 76-77 1.0058828277168885 2313 78-79 1.1039237491089544 2313 80-81 1.073425444538568 2313 82-83 1.023159975511927 2313 84-85 1.1520476665811934 2313 86-87 1.1507048480163427 2313 88-89 1.1236502410108855 2313 90-91 1.2778732146929137 2313 92-93 1.3095603181522364 2313 94-95 1.328571572995056 2313 96-97 1.4282899457923932 2313 98-99 1.4651016978676417 2313 100 1.6192334574403446 2314 1 0.47222352052517635 2314 2 0.45784606268710704 2314 3 0.5701635733937849 2314 4 0.49084678629441925 2314 5 0.4445585299206698 2314 6 0.4963137640257571 2314 7 0.527719213320097 2314 8 0.5559884504581802 2314 9 0.6625567173639269 2314 10-11 0.6390524834540869 2314 12-13 0.388739424501793 2314 14-15 0.8189789515121291 2314 16-17 0.8608596176006458 2314 18-19 0.8721417505153823 2314 20-21 0.8796779037921141 2314 22-23 0.7509672165015076 2314 24-25 0.7527582914335085 2314 26-27 0.967132610966992 2314 28-29 0.9385144857635268 2314 30-31 0.9107218386149967 2314 32-33 0.9057172404283094 2314 34-35 0.9555926901431633 2314 36-37 1.0658470821529065 2314 38-39 1.0829967879873763 2314 40-41 1.0622146862666924 2314 42-43 1.1536081628750168 2314 44-45 1.2143396113439024 2314 46-47 1.212817832228346 2314 48-49 1.2482506195006522 2314 50-51 1.1856465282046358 2314 52-53 1.025109293356678 2314 54-55 1.044916592168299 2314 56-57 1.0508662526054735 2314 58-59 0.9655253331537921 2314 60-61 0.9306691689475741 2314 62-63 0.8838241553969013 2314 64-65 0.8742371602308268 2314 66-67 0.8859913221727069 2314 68-69 0.9213871272582494 2314 70-71 0.984428116493163 2314 72-73 0.9679899981706654 2314 74-75 1.0271573321539833 2314 76-77 0.9283694568083121 2314 78-79 1.067899924212707 2314 80-81 1.138821020578252 2314 82-83 1.0368922663028606 2314 84-85 1.025784347866221 2314 86-87 1.1951271287923326 2314 88-89 1.2532471335132627 2314 90-91 1.2813857268220836 2314 92-93 1.4064639214111097 2314 94-95 1.5047254253809719 2314 96-97 1.518872320373518 2314 98-99 1.58988334167611 2314 100 1.5080219147721579 2315 1 0.3780463028317591 2315 2 0.35575142306464613 2315 3 0.49221528723924735 2315 4 0.386666022151573 2315 5 0.326157771774632 2315 6 -0.30008757870317737 2315 7 0.22161446639225346 2315 8 0.24936275743606728 2315 9 0.5670607534317256 2315 10-11 0.6069748370464154 2315 12-13 0.6895244029256702 2315 14-15 0.8041273075388133 2315 16-17 0.6214491623536915 2315 18-19 0.737385126928487 2315 20-21 0.8248094885401756 2315 22-23 0.6803154782883496 2315 24-25 0.7577164511810537 2315 26-27 0.8141537444236988 2315 28-29 0.9118669531653225 2315 30-31 0.9522634019756637 2315 32-33 1.0237675054986042 2315 34-35 0.9596610670167465 2315 36-37 0.9577787926949881 2315 38-39 0.9300136374720722 2315 40-41 0.9799162058198689 2315 42-43 1.0344271934034737 2315 44-45 0.9988268315948687 2315 46-47 0.9809842136994789 2315 48-49 1.0108752396802956 2315 50-51 0.9929031163119433 2315 52-53 0.8228939438585385 2315 54-55 0.717376849741072 2315 56-57 0.7212210983477547 2315 58-59 0.7356797229757674 2315 60-61 0.55864552927288 2315 62-63 0.6108040336369882 2315 64-65 0.7324576156196869 2315 66-67 0.7968762118825126 2315 68-69 0.8933657606930652 2315 70-71 0.8555191524459147 2315 72-73 0.869168520758187 2315 74-75 0.8819974127998478 2315 76-77 0.8387475356442735 2315 78-79 0.7834461264922759 2315 80-81 0.8869777614138705 2315 82-83 1.029156620666381 2315 84-85 0.9717659896684872 2315 86-87 1.1008113897131757 2315 88-89 1.1861906102958741 2315 90-91 1.284231737391078 2315 92-93 1.3521386081263103 2315 94-95 1.410563418619013 2315 96-97 1.4521932584962016 2315 98-99 1.5952903560065437 2315 100 1.5538257283300752 2316 1 0.10340749300113217 2316 2 -0.007434954493447776 2316 3 0.13450558524180778 2316 4 0.10093925266509274 2316 5 0.14095247211293582 2316 6 0.163369060049682 2316 7 0.18998153353386726 2316 8 0.30165868243917515 2316 9 0.3390532868054592 2316 10-11 0.2811267568933644 2316 12-13 0.3496009085958036 2316 14-15 0.5282735647321388 2316 16-17 0.5843346061614838 2316 18-19 0.4740724065274762 2316 20-21 0.410217494102632 2316 22-23 0.3344595857469983 2316 24-25 0.42840527543116025 2316 26-27 0.4777156470505801 2316 28-29 0.593697762388544 2316 30-31 0.5556994376189763 2316 32-33 0.6730178287318935 2316 34-35 0.6764759977080885 2316 36-37 0.7452259138240791 2316 38-39 0.8217943785051531 2316 40-41 0.7699111027011796 2316 42-43 0.8526716740078939 2316 44-45 0.7311786249607195 2316 46-47 0.644136142386941 2316 48-49 0.7172891871909002 2316 50-51 0.5084258996613613 2316 52-53 0.2678164244406247 2316 54-55 0.2957848840806605 2316 56-57 0.3734350649436635 2316 58-59 0.45887913646092215 2316 60-61 0.49848008010402367 2316 62-63 0.5163805850922003 2316 64-65 0.5523147974197613 2316 66-67 0.5054901357965988 2316 68-69 0.5257014847103605 2316 70-71 0.49035259239284557 2316 72-73 0.5100732239912986 2316 74-75 0.610473790075261 2316 76-77 0.6476031619153844 2316 78-79 0.7506523041654241 2316 80-81 0.8352019164933786 2316 82-83 0.8760635148171083 2316 84-85 0.9246355034657334 2316 86-87 0.9421520208663985 2316 88-89 1.0359074212246888 2316 90-91 1.0631257230926643 2316 92-93 1.0964591193418762 2316 94-95 1.1945761121310596 2316 96-97 1.3155454046455652 2316 98-99 1.3595514117238174 2316 100 1.3639822187552397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15.0 1 227.0 2 110.0 3 87.0 4 85.0 5 57.0 6 69.0 7 382.0 8 1427.0 9 2653.0 10 3771.0 11 4826.0 12 5954.0 13 7172.0 14 8489.0 15 10413.0 16 13844.0 17 18449.0 18 23336.0 19 28778.0 20 34990.0 21 41751.0 22 45628.0 23 46530.0 24 46001.0 25 48005.0 26 52803.0 27 61083.0 28 71779.0 29 85562.0 30 103928.0 31 129231.0 32 162843.0 33 210586.0 34 287682.0 35 386710.0 36 552403.0 37 792091.0 38 728877.0 39 187476.0 40 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.242928845431315 18.863726410722705 12.466612457201643 23.426732286644338 2 16.39837247395387 20.732553377902217 40.1020417880883 22.767032360055616 3 18.88311817399478 26.95120695554906 30.4033711480161 23.762303722440052 4 12.8973730717502 17.022267730196265 38.09198148651913 31.988377711534397 5 12.855943650182159 37.38471306680364 34.50791408650325 15.251429196510955 6 29.316885337382775 36.77462002008545 19.35489165416678 14.553602988364997 7 26.051521809253124 33.43260830883969 23.069561626447616 17.44630825545957 8 25.67053262499982 34.83475204600521 21.10642937779463 18.388285951200345 9 27.37940559424759 15.81800655429843 20.86665720077373 35.93593065068025 10-11 24.60653232567681 25.764025235017453 29.254068581689747 20.37537385761599 12-13 25.231519992446277 24.232174164634245 28.512096596294896 22.02420924662458 14-15 22.901971983570885 25.541599363304044 26.772701018984602 24.78372763414047 16-17 21.970806885621165 27.946902976818826 27.516507069889727 22.565783067670278 18-19 21.559383631047954 27.312831056845983 29.569864028987304 21.557921283118763 20-21 22.50050886761262 26.83224442005262 29.18807556731605 21.479171145018707 22-23 22.40951071712993 26.569426936679797 29.085598879693297 21.935463466496973 24-25 21.929093775751866 26.34387054071795 29.479886512565574 22.247149170964615 26-27 21.826122065808075 26.573331792494688 29.201900272547725 22.398645869149508 28-29 21.764089884787055 27.418543308706163 29.066497650586122 21.750869155920665 30-31 22.752257322186427 26.69687486290573 29.179640961324942 21.371226853582904 32-33 22.54854762854532 26.752848058184032 28.883373332675426 21.815230980595217 34-35 22.52830926301867 26.851786897755638 29.034689741320907 21.585214097904785 36-37 22.17017036773702 26.75087359686492 29.05999907260217 22.01895696279589 38-39 22.212756840459 26.836770871999054 29.027486913795965 21.92298537374598 40-41 22.208996855755323 26.23215571293623 29.51320386583367 22.04564356547478 42-43 21.907215299671883 26.648470324127555 28.85589554958523 22.58841882661533 44-45 21.30426737717874 26.990995957587128 29.363038737669356 22.341697927564773 46-47 21.319990548153577 26.414640420152885 29.0947645119081 23.170604519785435 48-49 21.56742918667149 26.516662302410378 29.025431841376054 22.890476669542082 50-51 21.500898962254762 26.88865602497333 28.879820856756627 22.730624156015285 52-53 21.312932679077733 27.05102399278102 28.595260333459148 23.04078299468209 54-55 21.05324619942667 26.76915532006537 29.054220379145228 23.123378101362736 56-57 21.582067425773175 26.59367355217156 28.57401086298706 23.25024815906821 58-59 21.19947444695746 26.666322577207847 28.855754289681624 23.278448686153073 60-61 21.62068489925784 26.670669561433336 28.59667228657239 23.111973252736433 62-63 21.74847125435022 26.469932191822355 28.491467203456633 23.290129350370787 64-65 21.358567272332092 26.28880915445473 28.486380999567324 23.866242573645856 66-67 21.417819750646345 26.304131649255414 28.20478947460417 24.07325912549407 68-69 21.13059101124103 26.51341550176304 28.416458403614804 23.939535083381124 70-71 21.336833100219017 26.424448962710667 28.363166495467766 23.875551441602546 72-73 21.53207125245736 26.867181588306792 27.909908017881495 23.69083914135435 74-75 21.962092389026527 26.877197921978723 27.603777705989213 23.55693198300553 76-77 21.98129473103775 26.719066434307216 27.601946759439244 23.69769207521579 78-79 21.871726811888326 26.832551394329094 27.701922045781657 23.593799748000926 80-81 22.0765087873714 26.926728330470173 27.55064662103607 23.446116261122356 82-83 22.542858685052718 26.751828019784536 27.636287372723434 23.069025922439316 84-85 22.717982614650108 27.101387594392556 27.010953816597873 23.169675974359464 86-87 22.516830118371665 27.18332646381639 27.200933192273443 23.098910225538503 88-89 22.72544991541515 26.5559444737246 27.22638148413979 23.492224126720455 90-91 23.40237733552427 26.34788461270955 27.13437727417613 23.11536077759005 92-93 23.617590567573238 26.491106570752414 26.89818136639169 22.993121495282658 94-95 23.44360587046963 26.324826200698702 26.851250435902713 23.380317492928953 96-97 23.635321452698967 26.112344069943603 26.745100445125487 23.507234032231946 98-99 23.71703613918901 26.210391555144785 26.508718605061237 23.563853700604966 100 24.185208331549795 26.10945598750477 26.381885528226988 23.323450152718443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2648.0 1 1877.5 2 2400.0 3 3469.5 4 2380.0 5 1610.5 6 2122.5 7 3356.5 8 5079.5 9 5984.0 10 5046.5 11 4046.0 12 4149.0 13 4496.5 14 4941.0 15 5479.0 16 5923.0 17 6485.5 18 7139.0 19 7620.0 20 8253.5 21 9268.0 22 10439.5 23 11885.5 24 14025.5 25 16623.5 26 19661.5 27 23523.0 28 28163.0 29 34174.5 30 41732.5 31 49112.0 32 57722.5 33 66666.0 34 74688.0 35 82579.5 36 91717.0 37 103362.0 38 111217.0 39 114158.5 40 116218.5 41 119196.0 42 123777.5 43 131037.0 44 139469.5 45 150316.5 46 162427.5 47 172054.5 48 181077.0 49 188123.0 50 189381.5 51 186480.0 52 179580.5 53 169378.0 54 157695.5 55 141499.5 56 125251.5 57 108469.0 58 90819.0 59 75631.5 60 59810.0 61 45904.5 62 35859.5 63 27356.5 64 19973.5 65 14236.5 66 10344.0 67 7863.0 68 5936.0 69 4332.5 70 3322.0 71 2492.5 72 1840.5 73 1349.5 74 1045.0 75 798.0 76 556.5 77 379.5 78 285.0 79 199.5 80 141.5 81 107.5 82 82.0 83 64.5 84 44.5 85 34.5 86 35.0 87 25.5 88 19.5 89 17.5 90 12.5 91 10.5 92 10.5 93 9.0 94 7.5 95 6.5 96 4.5 97 3.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2141546016493803 2 0.1442357868790842 3 0.02315570404157376 4 0.02232362022077799 5 0.027268575498650003 6 0.053942805411017314 7 0.05931568951101287 8 0.053752614823406854 9 0.04131890515837289 10-11 0.04355364456279582 12-13 0.04175872089222209 14-15 0.020112654639806365 16-17 0.018079992734719552 18-19 0.017628290089144703 20-21 0.021182476695115218 22-23 0.024487038154846996 24-25 0.021574744782061797 26-27 0.01913792787830275 28-29 0.01878132052653313 30-31 0.017010170679410706 32-33 0.024475151243121343 34-35 0.025152705211483613 36-37 0.0235836328636973 38-39 0.021967012869008373 40-41 0.023880805656838647 42-43 0.02970539240240905 44-45 0.03898907046014473 46-47 0.043684400591778014 48-49 0.04032040457341796 50-51 0.028064998584268815 52-53 0.043601192209698436 54-55 0.04146154809908074 56-57 0.043898365002839786 58-59 0.046929527492881526 60-61 0.024177978449979994 62-63 0.025247800505288843 64-65 0.026091771237810268 66-67 0.031274464750195366 68-69 0.0353873362072716 70-71 0.03913171340085258 72-73 0.043945912649742395 74-75 0.02511704447630665 76-77 0.017925462882286053 78-79 0.017497534060162512 80-81 0.02064756566746079 82-83 0.01901905876104621 84-85 0.011102375551760725 86-87 0.012564465694016154 88-89 0.0182226356754274 90-91 0.019030945672771863 92-93 0.01936377920109017 94-95 0.02266834066082195 96-97 0.023203251688476378 98-99 0.02830273681878189 100 0.028124433142897084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4206307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.52161183354545 #Duplication Level Percentage of deduplicated Percentage of total 1 81.01890943518329 53.895084446244354 2 11.99421697530235 15.957492917567691 3 3.243822751104989 6.473529537174888 4 1.2518210160462107 3.3309260685800193 5 0.6982081117235179 2.3222964493552296 6 0.4256720031401319 1.6989832656777357 7 0.26509140067235454 1.2344015079156068 8 0.19300541485030176 1.0271225030755347 9 0.14790178692840783 0.8854798733585363 >10 0.7191919504638099 8.16374921315223 >50 0.02774503821205652 1.24262931120219 >100 0.012931943104559437 1.538956934193374 >500 9.614097104491346E-4 0.41970155564504724 >1k 3.605286169068028E-4 0.5503286379169944 >5k 8.011747042373304E-5 0.3680849058467657 >10k+ 8.011747042373304E-5 0.8912328730938304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18639 0.4431202953089254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14806 0.3519952300200627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8781 0.20875794372593345 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5032 0.11962987960698065 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4418 0.10503275200787769 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3773823451307762E-5 2 0.0 0.0 0.0 0.0 2.3773823451307762E-5 3 0.0 0.0 0.0 0.0 2.3773823451307762E-5 4 0.0 0.0 0.0 0.0 2.3773823451307762E-5 5 0.0 0.0 0.0 0.0 2.3773823451307762E-5 6 0.0 0.0 0.0 2.3773823451307762E-5 2.3773823451307762E-5 7 0.0 0.0 0.0 2.3773823451307762E-5 2.3773823451307762E-5 8 0.0 0.0 0.0 2.3773823451307762E-5 2.3773823451307762E-5 9 0.0 0.0 0.0 1.188691172565388E-4 4.7547646902615525E-5 10-11 0.0 0.0 0.0 2.1396441106176985E-4 4.7547646902615525E-5 12-13 0.0 0.0 0.0 2.377382345130776E-4 4.7547646902615525E-5 14-15 0.0 0.0 0.0 2.615120579643854E-4 1.188691172565388E-4 16-17 0.0 0.0 0.0 2.9717279314134704E-4 1.4264294070784656E-4 18-19 0.0 0.0 0.0 5.111372042031169E-4 1.4264294070784656E-4 20-21 0.0 1.1886911725653881E-5 0.0 9.98500584954926E-4 1.5452985243350045E-4 22-23 0.0 2.3773823451307762E-5 0.0 0.0036017342528731257 1.6641676415915434E-4 24-25 0.0 2.3773823451307762E-5 0.0 0.009485755557071797 1.6641676415915434E-4 26-27 0.0 2.3773823451307762E-5 0.0 0.019221136260382327 1.6641676415915434E-4 28-29 0.0 2.3773823451307762E-5 0.0 0.05093541674442688 1.6641676415915434E-4 30-31 0.0 2.3773823451307762E-5 0.0 0.1402893321861671 1.6641676415915434E-4 32-33 0.0 2.3773823451307762E-5 0.0 0.33355862993357355 1.6641676415915434E-4 34-35 0.0 2.3773823451307762E-5 0.0 0.6184046956154174 1.6641676415915434E-4 36-37 0.0 2.3773823451307762E-5 0.0 1.0322713011675089 1.6641676415915434E-4 38-39 0.0 2.3773823451307762E-5 0.0 1.5997049193033224 2.1396441106176985E-4 40-41 0.0 2.3773823451307762E-5 0.0 2.214258255519628 2.377382345130776E-4 42-43 0.0 2.3773823451307762E-5 0.0 2.786624942021588 2.377382345130776E-4 44-45 0.0 2.3773823451307762E-5 0.0 3.4633111658278866 2.6151205796438534E-4 46-47 0.0 2.3773823451307762E-5 0.0 4.21990121025403 3.2094661659265477E-4 48-49 0.0 2.3773823451307762E-5 0.0 4.985406438474415 3.566073517696164E-4 50-51 0.0 2.3773823451307762E-5 0.0 5.756688705793467 4.04154998672232E-4 52-53 0.0 2.3773823451307762E-5 0.0 6.504660739218512 4.279288221235397E-4 54-55 0.0 2.3773823451307762E-5 0.0 7.3159662383178405 4.279288221235397E-4 56-57 0.0 2.3773823451307762E-5 0.0 8.19982944659056 4.3981573384919363E-4 58-59 0.0 2.3773823451307762E-5 0.0 9.13347504117032 4.517026455748475E-4 60-61 0.0 2.3773823451307762E-5 0.0 10.046698921405405 4.517026455748475E-4 62-63 1.1886911725653881E-5 2.3773823451307762E-5 0.0 10.962965851042256 4.992502924774631E-4 64-65 2.3773823451307762E-5 2.3773823451307762E-5 0.0 11.854650647230457 4.992502924774631E-4 66-67 2.3773823451307762E-5 2.3773823451307762E-5 0.0 12.825359632570803 5.111372042031169E-4 68-69 2.3773823451307762E-5 2.3773823451307762E-5 0.0 13.861268328726362 5.230241159287708E-4 70-71 2.3773823451307762E-5 2.3773823451307762E-5 0.0 14.915684946438764 5.230241159287708E-4 72-73 2.3773823451307762E-5 2.3773823451307762E-5 0.0 15.902429375697018 5.705717628313862E-4 74-75 2.3773823451307762E-5 2.3773823451307762E-5 0.0 16.877025381171656 5.705717628313862E-4 76-77 2.3773823451307762E-5 2.3773823451307762E-5 0.0 17.889326670640067 5.705717628313862E-4 78-79 2.3773823451307762E-5 2.3773823451307762E-5 0.0 18.939784946747825 5.824586745570401E-4 80-81 2.3773823451307762E-5 4.7547646902615525E-5 0.0 19.978962543627937 6.300063214596557E-4 82-83 2.3773823451307762E-5 4.7547646902615525E-5 0.0 20.996565395725987 6.894408800879251E-4 84-85 2.3773823451307762E-5 4.7547646902615525E-5 0.0 21.991060091429368 7.132147035392328E-4 86-87 2.3773823451307762E-5 4.7547646902615525E-5 0.0 22.975641102753556 7.369885269905407E-4 88 2.3773823451307762E-5 4.7547646902615525E-5 0.0 23.724587862940105 7.845361738931561E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7395 0.0 56.127644 1 TACCTGG 3435 0.0 44.394363 2 GTATCAA 13540 0.0 43.70288 1 TATCAAC 16905 0.0 34.718494 2 ATCAACG 16765 0.0 34.57379 3 ACCTGGG 4305 0.0 34.288143 3 CAACGCA 16930 0.0 34.20866 5 TCAACGC 17700 0.0 32.82672 4 AACGCAG 18200 0.0 32.10684 6 ACGCAGA 19695 0.0 29.240038 7 GTACATG 13550 0.0 28.928314 1 CGCAGAG 20125 0.0 28.471737 8 GTGGTAT 2830 0.0 27.968035 1 TACATGG 13845 0.0 27.638037 2 ACATGGG 13945 0.0 26.42926 3 GCAGAGT 21450 0.0 25.92348 9 CCTGGGG 4120 0.0 25.786568 4 GTACCTG 6180 0.0 25.462269 1 CATGGGG 11205 0.0 25.340046 4 ATGGGGG 8825 0.0 25.302372 5 >>END_MODULE