FastQCFastQC Report
Mon 6 Jun 2016
ERR1211390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206307
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168890.40151610426913686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142570.3389434009452948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.21013682548610935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47100.11197470845565956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71900.057.1541671
TACCTGG35350.043.7370722
GTATCAA128750.040.426461
ACCTGGG43550.034.856063
ATCAACG159800.032.1151353
CAACGCA162850.031.5679955
TATCAAC165950.031.1500452
TCAACGC166900.030.7778364
AACGCAG174250.030.0136726
GTACATG132250.028.619731
TACATGG134650.027.5368252
CCTGGGG40450.027.4200154
GTGGTAT26550.027.0951941
ACGCAGA193350.026.708557
ACATGGG133100.026.1639963
CGCAGAG198300.026.0889268
TGGGGGG85000.025.982196
ATGGGGG81650.025.668245
CATGGGG107000.024.6407174
GTACCTG65100.024.267421
Produced by FastQC (version 0.11.5)