##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4206307 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02756622376826 33.0 31.0 34.0 30.0 34.0 2 32.160741952501326 33.0 31.0 34.0 30.0 34.0 3 32.23363891413537 34.0 31.0 34.0 30.0 34.0 4 35.892984748854516 37.0 35.0 37.0 35.0 37.0 5 35.75029069442625 37.0 35.0 37.0 35.0 37.0 6 35.78949396703569 37.0 35.0 37.0 33.0 37.0 7 35.72087201433467 37.0 35.0 37.0 33.0 37.0 8 35.70695695773038 37.0 35.0 37.0 33.0 37.0 9 37.28199106722358 39.0 37.0 39.0 34.0 39.0 10-11 37.2576064942478 39.0 37.0 39.0 34.0 39.0 12-13 37.17317304704578 39.0 37.0 39.0 33.0 39.0 14-15 38.36201791737978 40.0 38.0 41.0 33.0 41.0 16-17 38.34429857354682 40.0 38.0 41.0 33.0 41.0 18-19 38.35634643405724 40.0 38.0 41.0 33.5 41.0 20-21 38.31924916559824 40.0 38.0 41.0 34.0 41.0 22-23 38.21595832163463 40.0 38.0 41.0 33.5 41.0 24-25 38.09388972321801 40.0 37.0 41.0 33.0 41.0 26-27 37.957818461657695 40.0 37.0 41.0 33.0 41.0 28-29 37.86982916368206 40.0 37.0 41.0 33.0 41.0 30-31 37.896344703322896 40.0 37.0 41.0 33.0 41.0 32-33 37.712770846255395 40.0 37.0 41.0 33.0 41.0 34-35 37.592714226517465 40.0 36.0 41.0 32.5 41.0 36-37 37.439508694919326 40.0 36.0 41.0 32.0 41.0 38-39 37.31484613462594 40.0 36.0 41.0 32.0 41.0 40-41 37.23767773013239 39.5 36.0 41.0 31.0 41.0 42-43 37.09316937636744 39.0 36.0 41.0 31.0 41.0 44-45 36.93511636216758 39.0 35.5 41.0 31.0 41.0 46-47 36.7043477806066 39.0 35.0 41.0 30.0 41.0 48-49 36.471637590884356 39.0 35.0 41.0 30.0 41.0 50-51 36.28544314525782 39.0 35.0 40.5 29.0 41.0 52-53 36.07600550791942 39.0 35.0 40.0 29.0 41.0 54-55 35.87144827992822 38.0 35.0 40.0 28.0 41.0 56-57 35.64303164272127 38.0 34.5 40.0 28.0 41.0 58-59 35.37788611244971 38.0 34.0 40.0 27.0 41.0 60-61 35.12054041704516 37.5 34.0 40.0 27.0 41.0 62-63 34.817773524376605 37.0 34.0 40.0 26.0 41.0 64-65 34.46492017819907 36.5 33.5 39.5 26.0 41.0 66-67 34.10736353765904 36.0 33.0 39.0 26.0 40.5 68-69 33.73595662418364 35.5 33.0 39.0 25.0 40.0 70-71 33.35814599362338 35.0 32.0 38.0 25.0 40.0 72-73 32.99527721110228 35.0 32.0 37.0 24.0 39.5 74-75 32.61844309034029 35.0 32.0 37.0 24.0 39.0 76-77 31.594645493065535 34.0 30.5 36.0 22.5 38.0 78-79 32.06922675876963 34.5 31.0 36.0 24.0 38.0 80-81 31.96015495302649 35.0 31.0 35.5 23.5 37.0 82-83 31.683274544630244 34.0 31.0 35.0 23.0 37.0 84-85 31.409540839506008 34.0 31.0 35.0 22.5 36.5 86-87 31.151012871861234 34.0 31.0 35.0 20.5 36.0 88-89 30.91784325300079 34.0 30.5 35.0 20.0 36.0 90-91 30.695415717397708 34.0 30.0 35.0 20.0 35.0 92-93 30.512191097796713 34.0 30.0 35.0 19.5 35.0 94-95 30.337549541676346 34.0 30.0 35.0 19.0 35.0 96-97 30.15827862778442 34.0 29.0 35.0 18.5 35.0 98-99 29.95159150295021 34.0 29.0 35.0 18.0 35.0 100 28.14564842746856 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3201993465743378 1101 2 -0.27480699076463466 1101 3 -0.26141857300715543 1101 4 -0.22537237070698524 1101 5 -0.1312267717474711 1101 6 -0.23266514990055498 1101 7 -0.24124452545415664 1101 8 -0.25432059102573845 1101 9 -0.44082068767932014 1101 10-11 -0.4630019202377511 1101 12-13 -0.49033142495357396 1101 14-15 -0.6284818284622133 1101 16-17 -0.6317302936338223 1101 18-19 -0.6262781559329724 1101 20-21 -0.6634103566870735 1101 22-23 -0.6989675905524138 1101 24-25 -0.7157261164195177 1101 26-27 -0.6968388182571843 1101 28-29 -0.6841163497449969 1101 30-31 -0.5228925797708328 1101 32-33 -0.4328155060369312 1101 34-35 -0.546391477790543 1101 36-37 -0.5778391285591695 1101 38-39 -0.628453389539672 1101 40-41 -0.6116937232056401 1101 42-43 -0.4311642701783356 1101 44-45 -0.5048039524678956 1101 46-47 -0.628284888354095 1101 48-49 -0.49883452256957383 1101 50-51 -0.454803356152766 1101 52-53 -0.6164652554909154 1101 54-55 -0.6105757027588652 1101 56-57 -0.5865051425429328 1101 58-59 -0.5940601517893782 1101 60-61 -0.5917955487206754 1101 62-63 -0.6879347058229754 1101 64-65 -0.5530920245381097 1101 66-67 -0.6909222981775827 1101 68-69 -0.7219716292653686 1101 70-71 -0.7357175281031871 1101 72-73 -0.7469158292427736 1101 74-75 -0.7659614413276614 1101 76-77 -0.5855652401965763 1101 78-79 -0.6293539544621183 1101 80-81 -0.5359629300391084 1101 82-83 -0.6383771929693935 1101 84-85 -0.6555010597472126 1101 86-87 -0.6398912549542324 1101 88-89 -0.7617039298297996 1101 90-91 -0.8476335038601199 1101 92-93 -0.8637850696649529 1101 94-95 -0.9411969826463711 1101 96-97 -0.8711263312541071 1101 98-99 -1.0571353159586963 1101 100 -0.893925603027693 1102 1 -0.33930493899082137 1102 2 -0.24540556471393415 1102 3 -0.34856180282267957 1102 4 -0.2201228595416893 1102 5 -0.24428469223585125 1102 6 -0.3378312387696951 1102 7 -0.3388288785800597 1102 8 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-0.9940503789446851 1102 72-73 -0.8329179929746928 1102 74-75 -0.7753068202594022 1102 76-77 -0.5557427993603739 1102 78-79 -0.6629795152097948 1102 80-81 -0.5378950658819193 1102 82-83 -0.6143551713914945 1102 84-85 -0.6469646407213752 1102 86-87 -0.7470196218527327 1102 88-89 -0.8098619277987815 1102 90-91 -0.6531994862991546 1102 92-93 -0.7774264041007548 1102 94-95 -0.9022397244837457 1102 96-97 -0.9357525887416749 1102 98-99 -1.0700484238414845 1102 100 -1.0539756260463484 1103 1 -0.26458606469280355 1103 2 -0.22080692895935528 1103 3 -0.30085940033292147 1103 4 -0.12911254715594112 1103 5 -0.16684042036955304 1103 6 -0.25170285630809275 1103 7 -0.3392007066797973 1103 8 -0.30499208054978055 1103 9 -0.3492361486133788 1103 10-11 -0.4513081933056071 1103 12-13 -0.5704308116421046 1103 14-15 -0.6726078393456447 1103 16-17 -0.7003273598117161 1103 18-19 -0.5894530580800961 1103 20-21 -0.5967140220250755 1103 22-23 -0.699941242874047 1103 24-25 -0.6989193176049753 1103 26-27 -0.8111126526121666 1103 28-29 -0.7067015769243312 1103 30-31 -0.7000195708450789 1103 32-33 -0.6375293128907558 1103 34-35 -0.7520370028873984 1103 36-37 -0.6215140193461011 1103 38-39 -0.6413436257739136 1103 40-41 -0.8142549018483933 1103 42-43 -0.7866991777052874 1103 44-45 -0.6709435209165449 1103 46-47 -0.7343151330525899 1103 48-49 -0.843473035104978 1103 50-51 -0.7792413682699646 1103 52-53 -0.8117035678093529 1103 54-55 -0.9220365786653346 1103 56-57 -1.0346126699949565 1103 58-59 -0.9486821529639684 1103 60-61 -0.7849020902835164 1103 62-63 -0.8374007293932877 1103 64-65 -0.9136386754334751 1103 66-67 -0.8956219321895134 1103 68-69 -0.7645589005483941 1103 70-71 -0.9527183487399569 1103 72-73 -1.0372456553821827 1103 74-75 -1.0073186560457046 1103 76-77 -0.955128375495125 1103 78-79 -0.855464223117103 1103 80-81 -0.7185865729255703 1103 82-83 -0.6825055416411772 1103 84-85 -0.7452617951452929 1103 86-87 -0.7611460175587617 1103 88-89 -0.7399588453403005 1103 90-91 -0.8600974589991317 1103 92-93 -0.7686217053800313 1103 94-95 -0.8087735457628931 1103 96-97 -1.029604967265648 1103 98-99 -1.0243081649431964 1103 100 -1.0954326582903064 1104 1 -0.32719689985736267 1104 2 -0.36358416199330534 1104 3 -0.4019738016656724 1104 4 -0.22031336459806283 1104 5 -0.23707484050179062 1104 6 -0.25593337107002867 1104 7 -0.32851150393764783 1104 8 -0.2733517970467503 1104 9 -0.4393731303160493 1104 10-11 -0.4412469341034324 1104 12-13 -0.5479347876503766 1104 14-15 -0.6690582815672883 1104 16-17 -0.6340579675919642 1104 18-19 -0.669603300137517 1104 20-21 -0.6873890062539303 1104 22-23 -0.6927899881059787 1104 24-25 -0.7648251690862082 1104 26-27 -0.8183991336245455 1104 28-29 -0.806807359026493 1104 30-31 -0.7850281313272731 1104 32-33 -0.7707043285919681 1104 34-35 -0.7980418664162414 1104 36-37 -0.735284471135337 1104 38-39 -0.8367206157013598 1104 40-41 -0.8207354806041209 1104 42-43 -0.7277293100430668 1104 44-45 -0.9264396092517728 1104 46-47 -0.8943478573141448 1104 48-49 -0.8514555448649048 1104 50-51 -0.856285917746618 1104 52-53 -0.7772717558266393 1104 54-55 -0.7578531395615542 1104 56-57 -0.7845081118179422 1104 58-59 -1.051139095277236 1104 60-61 -1.0314020839890432 1104 62-63 -1.030498525908385 1104 64-65 -0.9054929639972542 1104 66-67 -0.9995567466354771 1104 68-69 -0.8333860865136771 1104 70-71 -0.9931087179177425 1104 72-73 -0.7937815905239418 1104 74-75 -0.7534118520010189 1104 76-77 -0.6648474601949417 1104 78-79 -0.6631339675475445 1104 80-81 -0.8342684461045451 1104 82-83 -0.9173544323453591 1104 84-85 -0.7766879966551308 1104 86-87 -0.6630348138178874 1104 88-89 -0.8064748060236298 1104 90-91 -0.8375515338306165 1104 92-93 -1.0436690188293625 1104 94-95 -1.0584813773451955 1104 96-97 -1.0830974201934858 1104 98-99 -1.0310177575789403 1104 100 -1.1282987552839252 1105 1 -0.37500707097721175 1105 2 -0.4122308147554179 1105 3 -0.45333046170214075 1105 4 -0.2674239140671162 1105 5 -0.24051946086197518 1105 6 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1105 68-69 -1.037681612993289 1105 70-71 -0.9376771775906292 1105 72-73 -1.115721944965781 1105 74-75 -1.099165223808825 1105 76-77 -1.0955265977078312 1105 78-79 -0.9688409532735278 1105 80-81 -0.9060516496635955 1105 82-83 -0.8750599673416275 1105 84-85 -0.7366742209343577 1105 86-87 -0.8127080545493008 1105 88-89 -0.770522831940756 1105 90-91 -0.7880584955388024 1105 92-93 -0.923712720164005 1105 94-95 -0.9344928161626349 1105 96-97 -1.03769765512612 1105 98-99 -1.0126206482717706 1105 100 -0.9935238944559757 1106 1 -0.34365590116964384 1106 2 -0.3599490036400148 1106 3 -0.32168104086639815 1106 4 -0.20663247928074924 1106 5 -0.25463372411449114 1106 6 -0.35659695319592544 1106 7 -0.32528829412122917 1106 8 -0.3876157687988524 1106 9 -0.3997246056194328 1106 10-11 -0.42537068100874365 1106 12-13 -0.5510653259568556 1106 14-15 -0.6333564132555267 1106 16-17 -0.6237579013013956 1106 18-19 -0.6637108802611706 1106 20-21 -0.5725842252157349 1106 22-23 -0.5950908796464915 1106 24-25 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0.8669675991889889 2311 32-33 1.050873756131125 2311 34-35 1.0946597211398128 2311 36-37 1.095535970072774 2311 38-39 1.1668827104458472 2311 40-41 1.0921887782480937 2311 42-43 1.1075653349611727 2311 44-45 1.0936630355499588 2311 46-47 1.1848003342726088 2311 48-49 1.2675335325131627 2311 50-51 1.315744533966452 2311 52-53 1.2938212657612524 2311 54-55 1.270810832661489 2311 56-57 1.2344340307709984 2311 58-59 1.266861398329084 2311 60-61 1.2647431459597769 2311 62-63 1.3496653556092184 2311 64-65 1.3971833736969757 2311 66-67 1.4029869079919877 2311 68-69 1.4035197401503439 2311 70-71 1.418804491746819 2311 72-73 1.4335478516165452 2311 74-75 1.2451725103819626 2311 76-77 1.1449756220179346 2311 78-79 1.2403362536471505 2311 80-81 1.205626968419292 2311 82-83 1.2074269253673435 2311 84-85 1.2151722452541627 2311 86-87 1.2226116838976004 2311 88-89 1.2463684929541436 2311 90-91 1.3023125556766644 2311 92-93 1.297999054123121 2311 94-95 1.3022367354754927 2311 96-97 1.3574426357485656 2311 98-99 1.3572101535381726 2311 100 1.5180736601385298 2312 1 0.23498841155896955 2312 2 0.24587774390185047 2312 3 0.36443101406833023 2312 4 0.2735303407803258 2312 5 0.35407860683621095 2312 6 0.43350894381404004 2312 7 0.4964925332555268 2312 8 0.3767076984315878 2312 9 0.5112165123711065 2312 10-11 0.3649005424383489 2312 12-13 0.5136454759995885 2312 14-15 0.8804148647235905 2312 16-17 0.9378708970643217 2312 18-19 0.8989537645255652 2312 20-21 0.9214028318163869 2312 22-23 0.4688572079571145 2312 24-25 0.4662262647548232 2312 26-27 0.7297966659946908 2312 28-29 0.8727704993062702 2312 30-31 0.880866616921864 2312 32-33 0.7867548107634335 2312 34-35 0.8419627869800976 2312 36-37 0.8509697360449238 2312 38-39 0.7943774583450107 2312 40-41 0.8383684962436391 2312 42-43 0.7616882504723392 2312 44-45 0.7706791097255845 2312 46-47 1.0207984165690362 2312 48-49 1.0101057014803771 2312 50-51 1.0656548460053443 2312 52-53 1.0598243123721787 2312 54-55 1.1527538418078578 2312 56-57 1.1104892746306803 2312 58-59 1.1389450270794725 2312 60-61 1.1209789038058844 2312 62-63 1.092885534403031 2312 64-65 1.1131396386749444 2312 66-67 1.2232937667255115 2312 68-69 1.207362685329663 2312 70-71 1.1267992128295248 2312 72-73 1.181624997325379 2312 74-75 1.2509271744829675 2312 76-77 1.190747877059426 2312 78-79 1.0714637155285374 2312 80-81 0.9185066992344026 2312 82-83 0.961293809138283 2312 84-85 0.7990609026785158 2312 86-87 0.9132101290664529 2312 88-89 0.7026503129673252 2312 90-91 1.0608700298805438 2312 92-93 1.235017257263653 2312 94-95 1.2884117900240852 2312 96-97 1.3581111667039103 2312 98-99 1.331543862737913 2312 100 1.481656750597626 2313 1 -0.4162168924165286 2313 2 -0.11924553347579803 2313 3 0.22177679328910926 2313 4 0.09432356229680039 2313 5 0.22273219669612843 2313 6 0.35155412188924373 2313 7 0.18063147019194759 2313 8 0.06175136655386382 2313 9 0.24496059307206508 2313 10-11 0.36811495220980106 2313 12-13 0.5377369344865102 2313 14-15 0.5946513992043947 2313 16-17 0.7147828838221031 2313 18-19 0.608367576695926 2313 20-21 0.8583184339544161 2313 22-23 0.885794289707249 2313 24-25 0.7445888342268319 2313 26-27 0.9443845480907527 2313 28-29 0.7553277959943046 2313 30-31 0.7691411766961522 2313 32-33 0.9014691425561026 2313 34-35 0.8943570016627973 2313 36-37 0.7430231656938702 2313 38-39 0.48915695559448835 2313 40-41 0.7173715551388824 2313 42-43 0.7824842189689036 2313 44-45 0.8877181841882376 2313 46-47 0.9641379645756132 2313 48-49 0.9688850498612496 2313 50-51 0.8618701277214456 2313 52-53 0.7810634307865101 2313 54-55 0.8240014669992632 2313 56-57 0.9733250264879985 2313 58-59 0.9563519310379576 2313 60-61 0.9735329750076076 2313 62-63 1.0315188852225177 2313 64-65 1.0729464462232912 2313 66-67 1.1201809952175452 2313 68-69 1.1089087034490959 2313 70-71 0.9594243865095535 2313 72-73 1.0742353550353627 2313 74-75 0.6823027380569542 2313 76-77 0.8749452980881749 2313 78-79 0.7482708739814399 2313 80-81 0.8735878148982188 2313 82-83 0.9364411713284966 2313 84-85 0.9079303697289411 2313 86-87 0.9962105756722472 2313 88-89 0.9983963927608954 2313 90-91 1.0936667568980702 2313 92-93 0.8406399710673185 2313 94-95 0.9500498585603516 2313 96-97 1.1750466534333377 2313 98-99 1.2801115341494729 2313 100 1.366791334899787 2314 1 -0.3239414978173656 2314 2 -0.08199527593545497 2314 3 0.09683031119977414 2314 4 -0.13554941874586035 2314 5 0.18339661026135445 2314 6 0.257807413201796 2314 7 0.14867294213236448 2314 8 0.1766042304709785 2314 9 0.32042739077841276 2314 10-11 0.44533015746617366 2314 12-13 0.5362489885584552 2314 14-15 0.548344033768835 2314 16-17 0.8097880424189654 2314 18-19 0.841069684320054 2314 20-21 0.4821980633705394 2314 22-23 0.724595888919012 2314 24-25 0.8788861495129225 2314 26-27 0.83450222828953 2314 28-29 0.790209087503797 2314 30-31 0.8189274400273305 2314 32-33 0.8914258094076501 2314 34-35 0.90095847878829 2314 36-37 0.8573718161077082 2314 38-39 0.8149573249070201 2314 40-41 0.9020782240158596 2314 42-43 0.6697255256971104 2314 44-45 0.7350361812255173 2314 46-47 0.8414732774224731 2314 48-49 0.7458922363740541 2314 50-51 0.8094233542827709 2314 52-53 0.94145384355528 2314 54-55 1.0460681168185673 2314 56-57 0.9287234957666897 2314 58-59 0.989492916689855 2314 60-61 1.0670830229010804 2314 62-63 0.6105575944858046 2314 64-65 0.8009930798765978 2314 66-67 0.9056560473020809 2314 68-69 0.9229651632089286 2314 70-71 1.023921064192443 2314 72-73 1.0748177420806329 2314 74-75 1.0351418437422808 2314 76-77 0.8556164905887549 2314 78-79 0.8460886441713242 2314 80-81 0.8689390793542948 2314 82-83 0.8674468079252193 2314 84-85 0.791281851372684 2314 86-87 0.9761137433093516 2314 88-89 1.032238135858723 2314 90-91 1.0920899260557952 2314 92-93 1.1880184970952534 2314 94-95 1.27603465822731 2314 96-97 1.3076493517331649 2314 98-99 1.3430368340523167 2314 100 1.3401620845493873 2315 1 0.010028543215160113 2315 2 0.19091423630922577 2315 3 0.168331200605202 2315 4 -0.03836994817384465 2315 5 0.20192873348393903 2315 6 0.3469542664035501 2315 7 0.40867411069424264 2315 8 0.36781394263939404 2315 9 0.5559162376554383 2315 10-11 0.5423317394030533 2315 12-13 0.6563512762221606 2315 14-15 0.7542720961030085 2315 16-17 0.7924074018054981 2315 18-19 0.8129289327022633 2315 20-21 0.8152408037477912 2315 22-23 0.8936449535712825 2315 24-25 0.8341088284908551 2315 26-27 0.9521277196063949 2315 28-29 0.8924249993912099 2315 30-31 0.9442144284078395 2315 32-33 0.9754899657195608 2315 34-35 0.946320646914451 2315 36-37 0.911028074873812 2315 38-39 0.9068694726059903 2315 40-41 0.8848181951430192 2315 42-43 0.8972889677368912 2315 44-45 0.7910339232136963 2315 46-47 0.7789548771021586 2315 48-49 0.8303876207056007 2315 50-51 0.6987261214191918 2315 52-53 0.800575725918236 2315 54-55 0.7696190434700227 2315 56-57 0.8307823840385851 2315 58-59 0.9331187513283936 2315 60-61 0.9429558019918503 2315 62-63 0.8925773223467601 2315 64-65 0.9376658845126755 2315 66-67 1.0824954301323118 2315 68-69 0.9376158379401787 2315 70-71 0.5163391320010433 2315 72-73 0.7762116069193894 2315 74-75 0.9389418175024815 2315 76-77 0.9090518700226049 2315 78-79 0.8273931072322398 2315 80-81 0.9055598686438593 2315 82-83 0.8172228222414617 2315 84-85 0.7350490570875827 2315 86-87 0.8774424879991152 2315 88-89 0.8305281063366827 2315 90-91 1.0021066414729312 2315 92-93 1.074921749230139 2315 94-95 1.1313485990195709 2315 96-97 1.1635447049030638 2315 98-99 1.003430142691851 2315 100 0.9134311786844442 2316 1 0.5165121088046014 2316 2 0.5051650272162291 2316 3 0.49019675993113054 2316 4 0.35988468118647177 2316 5 0.4264815266419575 2316 6 0.4363634015135176 2316 7 0.5216458513059266 2316 8 0.5393256979845233 2316 9 0.5862829043912257 2316 10-11 0.550151185555869 2316 12-13 0.7082786648860733 2316 14-15 0.8590308425017312 2316 16-17 0.8844571197951581 2316 18-19 0.8835534104347929 2316 20-21 0.9079941423759763 2316 22-23 0.9375908383209861 2316 24-25 0.9901403840783729 2316 26-27 1.0301133196219396 2316 28-29 0.9922981472187047 2316 30-31 0.8743879064300657 2316 32-33 0.8128147149575042 2316 34-35 0.7196299246112474 2316 36-37 0.7255631626438515 2316 38-39 0.6695780007806391 2316 40-41 0.7739890585945588 2316 42-43 0.8869207453630068 2316 44-45 0.7858179334515114 2316 46-47 0.8315968188004348 2316 48-49 0.6496196004337023 2316 50-51 0.6938378670579581 2316 52-53 0.8550103804416125 2316 54-55 0.9014352513053083 2316 56-57 0.947783976748596 2316 58-59 0.8306768179030826 2316 60-61 0.8884447298395983 2316 62-63 0.8998394709761968 2316 64-65 0.9650248872569875 2316 66-67 0.9730958726014762 2316 68-69 0.8759038640417174 2316 70-71 0.7759443877439836 2316 72-73 0.8611280366659386 2316 74-75 0.9334700533343039 2316 76-77 0.8715785457051304 2316 78-79 0.7944409197835256 2316 80-81 0.7088317146176486 2316 82-83 0.756945193230365 2316 84-85 0.7873132035623307 2316 86-87 0.834274822482552 2316 88-89 0.6764940140695153 2316 90-91 0.8618673955397576 2316 92-93 0.8156991637225239 2316 94-95 0.7471397153874477 2316 96-97 0.860707298879376 2316 98-99 0.9820533978878423 2316 100 0.7256971667494696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 26.0 13 167.0 14 662.0 15 1885.0 16 4260.0 17 8647.0 18 13644.0 19 18567.0 20 24556.0 21 31320.0 22 37063.0 23 39075.0 24 39235.0 25 42508.0 26 49010.0 27 58793.0 28 71271.0 29 86272.0 30 105288.0 31 129774.0 32 162783.0 33 210615.0 34 292080.0 35 399350.0 36 594128.0 37 899748.0 38 778002.0 39 107565.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.180149918390235 19.677109829944385 13.30084483108849 23.841895420576886 2 16.006824039905798 21.231099211517186 39.61204914716143 23.150027601415587 3 18.34407236561668 26.831208468616296 31.152671452654314 23.672047713112715 4 12.777368415921792 17.473386922089443 38.00074802656279 31.748496635425976 5 12.6353764006597 37.58070041841183 34.54492631372732 15.238996867201138 6 29.392528885789837 37.1388251023998 19.001751417573658 14.4668945942367 7 26.05459717785093 33.58387827015585 22.799050376364207 17.56247417562902 8 25.762959838888634 35.17825595662489 20.79296614136519 18.26581806312129 9 27.224225473864045 15.810477681013605 20.590663772220978 36.374633072901375 10-11 24.631422564414795 25.912794305933744 29.121002236302513 20.33478089334895 12-13 25.12950836508735 24.246866361156098 28.45734437837411 22.166280895382442 14-15 22.80055502388022 25.65680262643786 26.584481322148378 24.958161027533546 16-17 21.944633578552573 28.059907224965198 27.424408697041393 22.57105049944084 18-19 21.51893573150985 27.364110608189087 29.527706845933977 21.58924681436709 20-21 22.388813367358438 26.884004634945725 29.1222036627863 21.60497833490954 22-23 22.247377568969647 26.694948799505124 29.032747728589474 22.024925902935756 24-25 21.840014197735805 26.427794278802214 29.434554726125985 22.297636797335997 26-27 21.825230974636654 26.678741394468208 29.091166928892005 22.404860702003134 28-29 21.726876986840715 27.55671028687059 28.9819280481196 21.7344846781691 30-31 22.692429168513613 26.75323745975798 29.132602923470053 21.421730448258355 32-33 22.54451829320051 26.821693574714384 28.78417677922783 21.84961135285727 34-35 22.458574200360705 26.901442093051987 29.032283761243484 21.60769994534383 36-37 22.133149618114754 26.785414901431825 29.069841826238346 22.011593654215076 38-39 22.162996341068315 26.818358266183566 29.068954960364053 21.949690432384074 40-41 22.130237216709265 26.233925456841362 29.613783034810986 22.02205429163839 42-43 21.86579602192604 26.625626763529304 28.90412712662154 22.60445008792312 44-45 21.211735030503558 27.035882663009588 29.379231998455502 22.373150308031356 46-47 21.25120327211551 26.42080623779118 29.053578109524423 23.27441238056889 48-49 21.440769762234556 26.567612393303214 29.00723667036132 22.98438117410091 50-51 21.32941309198456 26.85100217826851 28.865725195313637 22.9538595344333 52-53 21.155883252992822 27.02632012575491 28.55426794631306 23.263528674939206 54-55 20.893388390943908 26.677171757038774 28.981626394842003 23.44781345717531 56-57 21.36214710582017 26.55188801818656 28.414360478371957 23.671604397621312 58-59 20.800606472503375 26.745513481281275 28.71862949767847 23.73525054853688 60-61 21.38962671508682 26.871723244348424 28.257462875075408 23.48118716548935 62-63 21.38550987323451 26.72446687338976 28.058308653912217 23.831714599463517 64-65 20.893467865065304 26.509029483050888 28.0339132008291 24.563589451054703 66-67 20.958576470202086 26.597591773613587 27.786185592216956 24.657646163967364 68-69 20.694877558544736 26.785159548530586 27.8402373901357 24.679725502788976 70-71 20.89374524370486 26.68524924443364 27.707072990689408 24.71393252117209 72-73 20.92584561264396 27.113509859857043 27.163800024235364 24.796844503263628 74-75 21.20891876151723 27.163812244760127 26.848969338041567 24.77829965568107 76-77 20.99738375435471 27.04383266729234 26.968697202889413 24.990086375463534 78-79 20.876509881368417 27.152625639772182 26.923620129754333 25.047244349105068 80-81 20.792931433214203 27.395570020879756 26.75213121496638 25.059367330939658 82-83 21.16361394090911 27.105995530717227 26.898547471207323 24.831843057166346 84-85 21.254999460674874 27.352287053687647 26.310693848018772 25.082019637618707 86-87 20.852886173423123 27.460292259436 26.517843285911784 25.168978281229094 88-89 20.81085388597092 27.002823608890917 26.61717620080688 25.56914630433128 90-91 21.23138163182288 26.643311299841265 26.619874281981215 25.505432786354636 92-93 21.156468125608246 26.855864173494 26.50217259868247 25.485495102215282 94-95 20.7661992083705 26.608187621136242 26.718922075278982 25.906691095214274 96-97 20.741993490254938 26.54620115685214 26.760850852993904 25.950954499899016 98-99 20.661170093365495 26.6767457521363 26.58890647471066 26.073177679787552 100 20.540072689746513 26.809533603849207 26.75289409577118 25.89749961063311 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2564.0 1 1812.5 2 2071.0 3 3029.0 4 2167.5 5 1456.0 6 1930.5 7 3203.5 8 4768.5 9 5369.5 10 4515.5 11 3658.5 12 3655.0 13 3963.0 14 4467.0 15 4914.0 16 5452.5 17 6083.5 18 6661.0 19 7369.0 20 8058.0 21 8897.5 22 10173.0 23 11799.5 24 13873.5 25 16537.0 26 19857.5 27 23757.0 28 28348.0 29 34471.0 30 41947.0 31 49224.0 32 58137.0 33 66634.0 34 74050.0 35 82068.0 36 90695.5 37 101472.5 38 109603.5 39 112771.0 40 115010.0 41 118903.5 42 123920.5 43 131127.0 44 140203.5 45 151292.0 46 162312.5 47 172102.0 48 182152.0 49 189107.5 50 191736.0 51 191146.5 52 184825.0 53 173611.0 54 160981.0 55 144094.0 56 126254.0 57 108450.0 58 90527.0 59 75443.5 60 59738.5 61 45141.5 62 34470.5 63 25982.5 64 18634.0 65 13003.5 66 9373.0 67 7075.0 68 5333.0 69 3878.5 70 2893.0 71 2111.5 72 1540.0 73 1191.0 74 889.0 75 598.0 76 395.5 77 247.5 78 149.5 79 88.5 80 60.5 81 39.5 82 19.5 83 11.5 84 9.5 85 9.0 86 7.0 87 4.5 88 3.0 89 2.0 90 1.0 91 1.0 92 2.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05118504189066561 2 2.3773823451307762E-5 3 0.0 4 0.013646174661050657 5 0.0038989070460144735 6 0.0 7 2.1396441106176988E-4 8 5.705717628313864E-4 9 0.0021396441106176986 10-11 7.964230856188101E-4 12-13 3.5660735176961647E-4 14-15 3.566073517696165E-5 16-17 7.13214703539233E-5 18-19 0.0 20-21 2.3773823451307762E-5 22-23 0.0 24-25 5.94345586282694E-5 26-27 2.3773823451307762E-5 28-29 8.91518379424041E-4 30-31 9.15292202875349E-4 32-33 0.013408436426537579 34-35 0.02202644742763664 36-37 0.029669731667232087 38-39 0.020076993904629404 40-41 1.307560289821927E-4 42-43 0.0017949236705737362 44-45 0.009604624674328337 46-47 0.01396712127764331 48-49 0.005444205570349477 50-51 0.010543690700654994 52-53 0.015833366418570968 54-55 0.00973538070331053 56-57 0.0029479541079621627 58-59 0.008213856002426832 60-61 0.0016522807298658894 62-63 8.558576442470795E-4 64-65 0.019161701701754054 66-67 0.04481365720571513 68-69 0.05365751952960162 70-71 0.047797272048854256 72-73 0.04059380354310801 74-75 0.02838594520086147 76-77 0.02937255887409074 78-79 0.012195971430520881 80-81 0.0018900189643789671 82-83 0.027530087556614385 84-85 0.04703650969841241 86-87 0.047416890873633336 88-89 0.05865002245437625 90-91 0.03377071621258268 92-93 0.025901580650199808 94-95 0.007108373211941021 96-97 0.003601734252873126 98-99 0.021372667282725678 100 0.018115653469896513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4206307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.90771711340746 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53481629665956 53.078658902604744 2 12.237222982546887 16.130548611747773 3 3.3699595218870373 6.663190165564941 4 1.3119055574945526 3.458588014514323 5 0.6952172174565212 2.2910089850247335 6 0.4316449440752373 1.7069239720525993 7 0.28564589269709717 1.3178388083340915 8 0.2029346308122665 1.0699966592070913 9 0.14963307181942945 0.8875776751456623 >10 0.7374942474941248 8.326248887901198 >50 0.028276007362501396 1.2549935246417743 >100 0.013754110656460773 1.6296384350013529 >500 9.700664273656928E-4 0.44149686565202584 >1k 4.041943160722906E-4 0.678109099775848 >5k 4.0419431607228975E-5 0.2354667583154082 >10k+ 8.083886321445795E-5 0.8297146345165407 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16889 0.40151610426913686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14257 0.3389434009452948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8839 0.21013682548610935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4710 0.11197470845565956 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.3773823451307762E-5 7 0.0 0.0 0.0 0.0 2.3773823451307762E-5 8 0.0 0.0 0.0 0.0 2.3773823451307762E-5 9 0.0 0.0 0.0 9.509529380523105E-5 4.7547646902615525E-5 10-11 0.0 0.0 0.0 1.6641676415915432E-4 4.7547646902615525E-5 12-13 0.0 0.0 0.0 1.901905876104621E-4 5.94345586282694E-5 14-15 0.0 0.0 0.0 2.0207749933611596E-4 1.783036758848082E-4 16-17 0.0 0.0 0.0 2.496251462387315E-4 2.615120579643854E-4 18-19 0.0 0.0 0.0 4.6358955730050136E-4 2.615120579643854E-4 20-21 0.0 0.0 0.0 9.390660263266567E-4 3.2094661659265477E-4 22-23 0.0 0.0 0.0 0.0035779604294218182 3.5660735176961647E-4 24-25 0.0 1.1886911725653881E-5 0.0 0.009378773351540913 3.803811752209242E-4 26-27 0.0 2.3773823451307762E-5 0.0 0.019090380231400132 3.803811752209242E-4 28-29 0.0 2.3773823451307762E-5 0.0 0.050899756009249925 3.803811752209242E-4 30-31 0.0 2.3773823451307762E-5 0.0 0.1401585761571849 3.9226808694657806E-4 32-33 0.0 2.3773823451307762E-5 0.0 0.3342480708136615 4.04154998672232E-4 34-35 0.0 2.3773823451307762E-5 0.0 0.6196647082583369 4.04154998672232E-4 36-37 0.0 2.3773823451307762E-5 0.0 1.0346011358657368 4.04154998672232E-4 38-39 0.0 2.3773823451307762E-5 0.0 1.6042932672294246 4.754764690261553E-4 40-41 0.0 2.3773823451307762E-5 0.0 2.2202135982941806 5.230241159287708E-4 42-43 0.0 2.3773823451307762E-5 0.0 2.7942087917025553 5.230241159287708E-4 44-45 0.0 2.3773823451307762E-5 0.0 3.4717270993296494 5.349110276544247E-4 46-47 0.0 2.3773823451307762E-5 0.0 4.23007640669119 5.705717628313862E-4 48-49 0.0 2.3773823451307762E-5 0.0 4.9964731532909985 5.943455862826941E-4 50-51 0.0 2.3773823451307762E-5 0.0 5.767089753553414 5.943455862826941E-4 52-53 0.0 2.3773823451307762E-5 0.0 6.5142891377162915 6.181194097340018E-4 54-55 0.0 2.3773823451307762E-5 0.0 7.323787826233321 6.656670566366174E-4 56-57 0.0 2.3773823451307762E-5 0.0 8.20699725436113 6.775539683622712E-4 58-59 0.0 2.3773823451307762E-5 0.0 9.13976321747319 6.894408800879251E-4 60-61 0.0 2.3773823451307762E-5 0.0 10.049135738309163 6.894408800879251E-4 62-63 0.0 2.3773823451307762E-5 0.0 10.962466600749778 7.369885269905407E-4 64-65 0.0 2.3773823451307762E-5 0.0 11.850050412392628 7.369885269905407E-4 66-67 0.0 2.3773823451307762E-5 0.0 12.817977860389172 7.488754387161945E-4 68-69 0.0 2.3773823451307762E-5 0.0 13.851628043316857 7.845361738931561E-4 70-71 0.0 2.3773823451307762E-5 0.0 14.904071433682802 7.845361738931561E-4 72-73 0.0 2.3773823451307762E-5 0.0 15.889817362356101 8.08309997344464E-4 74-75 0.0 2.3773823451307762E-5 0.0 16.863236563569895 8.08309997344464E-4 76-77 0.0 2.3773823451307762E-5 0.0 17.87573993053764 8.08309997344464E-4 78-79 0.0 2.3773823451307762E-5 0.0 18.92634084958611 8.08309997344464E-4 80-81 0.0 2.3773823451307762E-5 0.0 19.964222773088125 8.439707325214256E-4 82-83 0.0 3.566073517696165E-5 0.0 20.980446743426004 9.509529380523104E-4 84-85 0.0 4.7547646902615525E-5 0.0 21.974465962660357 9.509529380523104E-4 86-87 1.1886911725653881E-5 4.7547646902615525E-5 0.0 22.95790583045888 9.747267615036183E-4 88 4.7547646902615525E-5 4.7547646902615525E-5 0.0 23.70575899476667 9.985005849549261E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7190 0.0 57.154167 1 TACCTGG 3535 0.0 43.737072 2 GTATCAA 12875 0.0 40.42646 1 ACCTGGG 4355 0.0 34.85606 3 ATCAACG 15980 0.0 32.115135 3 CAACGCA 16285 0.0 31.567995 5 TATCAAC 16595 0.0 31.150045 2 TCAACGC 16690 0.0 30.777836 4 AACGCAG 17425 0.0 30.013672 6 GTACATG 13225 0.0 28.61973 1 TACATGG 13465 0.0 27.536825 2 CCTGGGG 4045 0.0 27.420015 4 GTGGTAT 2655 0.0 27.095194 1 ACGCAGA 19335 0.0 26.70855 7 ACATGGG 13310 0.0 26.163996 3 CGCAGAG 19830 0.0 26.088926 8 TGGGGGG 8500 0.0 25.98219 6 ATGGGGG 8165 0.0 25.66824 5 CATGGGG 10700 0.0 24.640717 4 GTACCTG 6510 0.0 24.26742 1 >>END_MODULE