FastQCFastQC Report
Mon 6 Jun 2016
ERR1211389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4212449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160620.38129838485878403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128980.3061876832218028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79340.188346493927879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42200.048.972882
GGTATCA90300.044.885421
ACCTGGG48800.041.80553
GTATCAA146150.035.0530131
ATCAACG173400.029.359113
CCTGGGG48450.029.3002664
CAACGCA174250.029.1619425
GTACCTG71450.029.1551071
TATCAAC177150.028.9926012
TCAACGC179400.028.4816724
GTACATG130750.028.1877021
AACGCAG184500.027.873386
ATAACGC4800.027.4208153
TACATGG133650.026.9813352
ACATGGG132100.025.9410933
ACGCAGA200300.025.3229527
TAACGCA4900.024.9422534
CTGGGGG36050.024.7748975
CGCAGAG204600.024.7206488
ATGGGGG67950.024.3509965
Produced by FastQC (version 0.11.5)