FastQCFastQC Report
Mon 6 Jun 2016
ERR1211389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4212449
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142790.33897146291860153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121590.28864444412264695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77840.18478561995646714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42200.047.437692
GGTATCA82250.045.6136131
ACCTGGG48650.040.568863
GTATCAA137400.036.3041421
CCTGGGG43250.029.8794774
GTACCTG69750.029.4553871
TAACGCA4650.029.3070054
ATCAACG166950.029.0200823
CAACGCA167150.028.9822625
TATCAAC172950.028.5839042
TCAACGC171000.028.4152074
AACGCAG177950.027.6180526
CTGGGGG30400.026.8940165
ATAACGC5250.026.8527223
GTACATG130650.026.1249071
TACATGG132200.025.1312642
ACGCAGA195250.024.9543957
CGCAGAG198650.024.3617218
ACATGGG131850.024.2356783
CATGGGG91050.023.8445114
Produced by FastQC (version 0.11.5)