FastQCFastQC Report
Mon 6 Jun 2016
ERR1211388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702493
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.41291503807780444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90810.33602307203015885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.19567118212702123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.1112676332556643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27120.10035178629509862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56600.048.3571431
TACCTGG22100.043.0139242
GTATCAA98450.039.2372971
ACCTGGG26350.035.8570633
ATCAACG115000.032.9455263
CAACGCA115900.032.9330445
TATCAAC117700.032.3061262
TCAACGC119600.031.7957134
AACGCAG123050.031.2110046
GTACATG92000.028.4188251
ACGCAGA134000.028.3804367
CGCAGAG135850.027.8555418
TACATGG93050.027.765532
ATAACGC3050.027.7416273
GTACACG3400.027.7109891
ACATGGG94050.026.6395993
CATGGGG70200.026.382164
ATGGGGG55650.026.1851965
CCTGGGG26200.025.6558254
TAACGCA2750.025.6395154
Produced by FastQC (version 0.11.5)