##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211388_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2702493 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.465062814223757 31.0 31.0 34.0 30.0 34.0 2 31.510342857502312 31.0 31.0 34.0 28.0 34.0 3 31.572816284815538 33.0 31.0 34.0 28.0 34.0 4 35.2111568836626 37.0 35.0 37.0 32.0 37.0 5 35.269934464215076 37.0 35.0 37.0 33.0 37.0 6 35.33111390112759 37.0 35.0 37.0 32.0 37.0 7 35.26866600579539 37.0 35.0 37.0 32.0 37.0 8 35.271088583763216 37.0 35.0 37.0 32.0 37.0 9 36.85216538951257 39.0 37.0 39.0 33.0 39.0 10-11 36.78284846621249 39.0 37.0 39.0 32.0 39.0 12-13 36.699907085790784 39.0 37.0 39.0 32.0 39.0 14-15 37.898537202501544 40.0 38.0 41.0 32.0 41.0 16-17 37.88651755989747 40.0 37.5 41.0 32.0 41.0 18-19 37.89497678624885 40.0 37.5 41.0 32.0 41.0 20-21 37.87581854976128 40.0 37.5 41.0 32.0 41.0 22-23 37.757228048324265 40.0 37.0 41.0 32.0 41.0 24-25 37.66836768864896 40.0 37.0 41.0 32.0 41.0 26-27 37.533785286400374 40.0 37.0 41.0 32.0 41.0 28-29 37.39054125209575 40.0 36.5 41.0 31.0 41.0 30-31 37.22173711458272 39.5 36.0 41.0 31.0 41.0 32-33 37.000738762320566 39.0 36.0 41.0 30.5 41.0 34-35 36.87349994986111 39.0 36.0 41.0 30.0 41.0 36-37 36.74608389364931 39.0 35.0 41.0 30.0 41.0 38-39 36.591186545164035 39.0 35.0 41.0 30.0 41.0 40-41 36.42640350964831 39.0 35.0 40.5 30.0 41.0 42-43 36.227596704228276 39.0 35.0 40.0 29.0 41.0 44-45 36.08640151889385 39.0 35.0 40.0 28.0 41.0 46-47 35.859010550628625 38.5 35.0 40.0 27.5 41.0 48-49 35.61065153545263 38.0 34.5 40.0 26.5 41.0 50-51 34.935186141092686 37.5 33.5 39.5 25.5 40.5 52-53 35.23228663311987 38.0 34.0 40.0 26.5 40.5 54-55 35.64830177173447 39.0 35.0 40.0 27.0 41.0 56-57 35.57260481340747 38.5 34.5 40.0 26.5 41.0 58-59 35.381682209722655 38.0 34.0 40.0 26.0 41.0 60-61 35.159238710331536 38.0 34.0 40.0 26.0 41.0 62-63 34.872205034388614 37.5 34.0 40.0 26.0 41.0 64-65 34.65446737512363 37.0 34.0 40.0 26.0 41.0 66-67 34.448486268049535 36.5 33.5 40.0 25.5 41.0 68-69 34.10500841260273 36.0 33.0 39.0 25.0 41.0 70-71 33.72536358096025 35.5 33.0 39.0 24.0 41.0 72-73 33.321297409466 35.0 32.5 38.5 23.5 40.0 74-75 32.92783219049966 35.0 32.0 37.5 23.0 40.0 76-77 32.51662816517934 35.0 32.0 37.0 22.0 39.0 78-79 32.089941768581824 35.0 31.0 36.5 20.5 39.0 80-81 31.67276066950035 35.0 31.0 36.0 20.0 38.5 82-83 31.27640349114688 35.0 31.0 35.5 18.5 37.0 84-85 30.915213101384538 34.5 30.0 35.0 18.0 37.0 86-87 30.552922986294504 34.0 30.0 35.0 17.0 36.5 88-89 30.21321775856589 34.0 29.0 35.0 14.5 36.0 90-91 29.891590283490096 34.0 29.0 35.0 11.5 36.0 92-93 29.5838479507625 34.0 29.0 35.0 10.0 35.0 94-95 29.2733620401607 34.0 27.5 35.0 10.0 35.0 96-97 28.951619301141577 34.0 27.0 35.0 10.0 35.0 98-99 28.60694680800283 34.0 25.5 35.0 9.0 35.0 100 27.32731962672984 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4621651099033173 1101 2 -0.4176361494685068 1101 3 -0.46715431984134526 1101 4 -0.3808179397892957 1101 5 -0.3425100629596045 1101 6 -0.32637186614035585 1101 7 -0.2741193234423065 1101 8 -0.34883015428528097 1101 9 -0.4759676417258589 1101 10-11 -0.45133041850053246 1101 12-13 -0.5468190154729413 1101 14-15 -0.7284422714545329 1101 16-17 -0.6880802663224159 1101 18-19 -0.6742939712896785 1101 20-21 -0.7215454919446529 1101 22-23 -0.6813301619133654 1101 24-25 -0.7221600546879117 1101 26-27 -0.7199589925493584 1101 28-29 -0.8331106496872067 1101 30-31 -0.8410845861609388 1101 32-33 -0.8383377519545121 1101 34-35 -0.8200160498248579 1101 36-37 -0.897122248183976 1101 38-39 -0.8432919312591665 1101 40-41 -1.0629216359252638 1101 42-43 -0.9509344006905636 1101 44-45 -1.0340890180313451 1101 46-47 -1.2213454211236296 1101 48-49 -1.258766918556347 1101 50-51 -1.0474733389879063 1101 52-53 -0.7778986955092861 1101 54-55 -0.797641369672192 1101 56-57 -0.939462770179496 1101 58-59 -0.8379390510333167 1101 60-61 -0.872756237119674 1101 62-63 -0.9220716379302729 1101 64-65 -0.7699515521841036 1101 66-67 -0.954836344782656 1101 68-69 -1.0721115882728398 1101 70-71 -0.9691722702413514 1101 72-73 -1.0510208628284516 1101 74-75 -1.0944847589208244 1101 76-77 -1.1215908411915336 1101 78-79 -1.0669568140127872 1101 80-81 -1.1082123936480883 1101 82-83 -1.134879061568892 1101 84-85 -1.095659032447461 1101 86-87 -1.2735580897649648 1101 88-89 -1.4463735391183086 1101 90-91 -1.5383846684849978 1101 92-93 -1.5052708532083194 1101 94-95 -1.6851760468900032 1101 96-97 -1.9098417109663437 1101 98-99 -1.845615889000232 1101 100 -1.7636851489890333 1102 1 -0.20741071551901769 1102 2 -0.2079807863268357 1102 3 -0.40978924228381786 1102 4 -0.27076433684153756 1102 5 -0.1896357573983991 1102 6 -0.23210941504592597 1102 7 -0.3757256223987113 1102 8 -0.38020357730933085 1102 9 -0.44507217154097845 1102 10-11 -0.4231463301980156 1102 12-13 -0.40062368600596443 1102 14-15 -0.5996085219324101 1102 16-17 -0.6572667866858382 1102 18-19 -0.5937891763067213 1102 20-21 -0.6638750646665343 1102 22-23 -0.5589852973035079 1102 24-25 -0.6387012411106596 1102 26-27 -0.6607352998837754 1102 28-29 -0.6774714406357063 1102 30-31 -0.6535388528417059 1102 32-33 -0.7522901356182246 1102 34-35 -0.785711383358823 1102 36-37 -0.7041195883715474 1102 38-39 -0.7481096893707857 1102 40-41 -1.00124847562887 1102 42-43 -0.8651995359993663 1102 44-45 -0.8455780249533689 1102 46-47 -0.9627842413707413 1102 48-49 -0.876073756795833 1102 50-51 -0.9511487605315736 1102 52-53 -0.7638862017114505 1102 54-55 -0.5792719892376823 1102 56-57 -0.7089112861343025 1102 58-59 -0.6309354476788656 1102 60-61 -0.7770595208085993 1102 62-63 -0.7354493556973267 1102 64-65 -0.7298868056796906 1102 66-67 -0.798576268811253 1102 68-69 -0.8161064313276327 1102 70-71 -0.839756371558245 1102 72-73 -0.7986448450638335 1102 74-75 -0.9629909529488536 1102 76-77 -0.9936808681150175 1102 78-79 -1.0203761777726363 1102 80-81 -1.0506944785506036 1102 82-83 -1.2375065820868656 1102 84-85 -1.2718120652030116 1102 86-87 -1.3495111078793478 1102 88-89 -1.291886427414319 1102 90-91 -1.2657412053604098 1102 92-93 -1.398916829733647 1102 94-95 -1.395148391347913 1102 96-97 -1.6732394383315032 1102 98-99 -1.655841851047068 1102 100 -1.46399619249771 1103 1 -0.5293093344272961 1103 2 -0.6533668437562703 1103 3 -0.5918711010261823 1103 4 -0.4163254091086799 1103 5 -0.4413149718578353 1103 6 -0.4891155163642651 1103 7 -0.5560610046373924 1103 8 -0.5438491266983689 1103 9 -0.6420596664754612 1103 10-11 -0.6305440034319858 1103 12-13 -0.740732460247834 1103 14-15 -0.8540712667712569 1103 16-17 -0.9039497497941937 1103 18-19 -0.9151249971673749 1103 20-21 -0.8777438305765912 1103 22-23 -0.8262264118706355 1103 24-25 -0.8215531083918037 1103 26-27 -0.9255435479795722 1103 28-29 -0.8792163482480362 1103 30-31 -0.9835535637017117 1103 32-33 -0.9952621699629205 1103 34-35 -1.0552449152403724 1103 36-37 -1.0037046499889186 1103 38-39 -0.9429133376106549 1103 40-41 -1.036953188244695 1103 42-43 -1.0407921930537398 1103 44-45 -0.9438986102099349 1103 46-47 -1.008045588627823 1103 48-49 -1.0173407400447445 1103 50-51 -0.8372027684481296 1103 52-53 -0.8108122428377698 1103 54-55 -0.840614739924817 1103 56-57 -0.8227537146980026 1103 58-59 -0.6924167114442099 1103 60-61 -0.6479023759772247 1103 62-63 -0.6926767040773356 1103 64-65 -0.5773883962684678 1103 66-67 -0.6649973539099321 1103 68-69 -0.7521832048591719 1103 70-71 -0.8555758869644237 1103 72-73 -0.8458964311462296 1103 74-75 -0.8632148037025544 1103 76-77 -0.8848060669803388 1103 78-79 -0.9484865716444837 1103 80-81 -1.0078686682182187 1103 82-83 -0.9746532727609072 1103 84-85 -1.1590479156389257 1103 86-87 -1.238140183909156 1103 88-89 -1.3145186866245453 1103 90-91 -1.169824862653119 1103 92-93 -1.1792389932281466 1103 94-95 -1.3896526817650567 1103 96-97 -1.3120283243051425 1103 98-99 -1.4127302020621428 1103 100 -1.3342854293021666 1104 1 -0.3598875588829067 1104 2 -0.4819055372236072 1104 3 -0.47265636065767325 1104 4 -0.27876896019745345 1104 5 -0.3507467976534855 1104 6 -0.35192288654851467 1104 7 -0.45673973160557324 1104 8 -0.40882199101997685 1104 9 -0.4302941723381082 1104 10-11 -0.4971426633984919 1104 12-13 -0.5166108522076343 1104 14-15 -0.8256545163524933 1104 16-17 -0.8435782255060928 1104 18-19 -0.8134409100651894 1104 20-21 -0.8092352878630251 1104 22-23 -0.7200036313011182 1104 24-25 -0.7755437281572952 1104 26-27 -0.6853345027534417 1104 28-29 -0.8878330986667962 1104 30-31 -0.8424600963650235 1104 32-33 -0.8313908131789987 1104 34-35 -0.6655752334983234 1104 36-37 -0.9269549012452032 1104 38-39 -0.9530674414632543 1104 40-41 -0.8557257175579238 1104 42-43 -0.8974568496701565 1104 44-45 -0.8508808547660394 1104 46-47 -0.8549454211236309 1104 48-49 -0.8466526328420656 1104 50-51 -0.9618366042940281 1104 52-53 -0.6557640016317379 1104 54-55 -0.6317638186517911 1104 56-57 -0.5426954232407155 1104 58-59 -0.6365349694006639 1104 60-61 -0.6088501146706946 1104 62-63 -0.7071410256853738 1104 64-65 -0.7231066542249209 1104 66-67 -0.7043832835581654 1104 68-69 -0.7292177107218194 1104 70-71 -0.6819232906495145 1104 72-73 -0.5862780056855996 1104 74-75 -0.6680888405534802 1104 76-77 -0.8017785962935733 1104 78-79 -0.7444751813597286 1104 80-81 -0.6825674956889074 1104 82-83 -0.7555239595280803 1104 84-85 -0.8918549508148068 1104 86-87 -0.9993703346629239 1104 88-89 -0.9635572125876948 1104 90-91 -0.8683397705258145 1104 92-93 -0.9716463634124004 1104 94-95 -0.8816372713797982 1104 96-97 -0.9923315068847103 1104 98-99 -0.8577097665512525 1104 100 -0.9429259653155668 1105 1 -0.6270129719434898 1105 2 -0.5018529801819085 1105 3 -0.5573311049535015 1105 4 -0.46403379103931286 1105 5 -0.4330722107140659 1105 6 -0.43931234366889527 1105 7 -0.4879878826677242 1105 8 -0.6147949257170637 1105 9 -0.7467237553436163 1105 10-11 -0.6732251181585198 1105 12-13 -0.7334132125538204 1105 14-15 -0.9162535722140248 1105 16-17 -1.0012787762535282 1105 18-19 -0.8738281641957997 1105 20-21 -1.035051810286312 1105 22-23 -0.9921082733152815 1105 24-25 -1.0274806282359705 1105 26-27 -0.9525031888274 1105 28-29 -1.06909934571636 1105 30-31 -0.9682078540361516 1105 32-33 -1.093390498466178 1105 34-35 -1.1774952964408882 1105 36-37 -1.18694530250405 1105 38-39 -1.1685347900077048 1105 40-41 -1.0784679677546194 1105 42-43 -1.046608383895176 1105 44-45 -1.035608470816392 1105 46-47 -1.004689244884446 1105 48-49 -1.1403775738334048 1105 50-51 -1.265675943397099 1105 52-53 -0.9789216727568331 1105 54-55 -0.8987677525620938 1105 56-57 -0.9338570282761154 1105 58-59 -1.029442286473838 1105 60-61 -0.9958844183685684 1105 62-63 -0.8337943695091354 1105 64-65 -0.8612078343980158 1105 66-67 -0.8342839916620264 1105 68-69 -0.83825956752748 1105 70-71 -0.6786509066998647 1105 72-73 -0.8265215934117975 1105 74-75 -0.7412899420483683 1105 76-77 -0.8311202170646226 1105 78-79 -0.9098696738852965 1105 80-81 -0.944543420157828 1105 82-83 -1.0215378068923577 1105 84-85 -0.8105375629312519 1105 86-87 -0.9705275337187835 1105 88-89 -1.0272723974814788 1105 90-91 -1.0556755691096065 1105 92-93 -0.7897426655367141 1105 94-95 -1.0018958079651625 1105 96-97 -1.067085244099502 1105 98-99 -1.1732995383844553 1105 100 -1.135702361853724 1106 1 -0.3842285755689758 1106 2 -0.5031039850311352 1106 3 -0.558523337762832 1106 4 -0.5083883626033554 1106 5 -0.40759476040086184 1106 6 -0.39218831146120436 1106 7 -0.5947400420440658 1106 8 -0.5223529184549776 1106 9 -0.7136989065251456 1106 10-11 -0.7143602031734204 1106 12-13 -0.6981997540314566 1106 14-15 -0.8767689129376706 1106 16-17 -0.7797845894952218 1106 18-19 -0.7797161914000768 1106 20-21 -0.801363188522707 1106 22-23 -0.7470758082510542 1106 24-25 -0.7816658080952053 1106 26-27 -0.7736200285586463 1106 28-29 -0.8452168006458365 1106 30-31 -0.9112842329579607 1106 32-33 -1.0865670646341812 1106 34-35 -0.9501638248836528 1106 36-37 -0.9775209082300691 1106 38-39 -1.0239766382036493 1106 40-41 -0.988717490937816 1106 42-43 -0.6718764072953007 1106 44-45 -0.8703708043197835 1106 46-47 -0.8685359527495535 1106 48-49 -0.731702225391544 1106 50-51 -0.8911119632385365 1106 52-53 -0.7691151075688722 1106 54-55 -0.7099531861333546 1106 56-57 -0.763117153935319 1106 58-59 -0.7412739682365199 1106 60-61 -0.6327314495562177 1106 62-63 -0.7954207307688037 1106 64-65 -0.7790881055248846 1106 66-67 -0.9371658213928598 1106 68-69 -0.8112593870896845 1106 70-71 -0.7619580597608788 1106 72-73 -0.6368659761939455 1106 74-75 -0.6735440986054755 1106 76-77 -0.7968924496113843 1106 78-79 -0.7055255500054365 1106 80-81 -0.8744812713970944 1106 82-83 -0.7638527138936198 1106 84-85 -0.7671429601279627 1106 86-87 -0.7206546187141463 1106 88-89 -0.7799848416136932 1106 90-91 -0.811929448445877 1106 92-93 -0.8033051915070857 1106 94-95 -0.9229753389001729 1106 96-97 -1.0217432259378718 1106 98-99 -0.895416479783691 1106 100 -0.7008753638409821 1107 1 -0.5103046263770885 1107 2 -0.4720520073554937 1107 3 -0.45105387811306485 1107 4 -0.410502396970152 1107 5 -0.4044166192205907 1107 6 -0.48485384077660143 1107 7 -0.5065361629476968 1107 8 -0.37095108333960525 1107 9 -0.5391677177221226 1107 10-11 -0.5473654717770771 1107 12-13 -0.6487149639221386 1107 14-15 -0.8546324837691017 1107 16-17 -0.7561279539777743 1107 18-19 -0.7964042925322232 1107 20-21 -0.8413106951554923 1107 22-23 -0.7641114668325386 1107 24-25 -0.8058278243559016 1107 26-27 -0.8789982731180643 1107 28-29 -0.7313510195356869 1107 30-31 -0.8619081956668069 1107 32-33 -0.823884529237958 1107 34-35 -0.910721548471173 1107 36-37 -0.8664507895306954 1107 38-39 -0.7293521582980063 1107 40-41 -0.7958642745788751 1107 42-43 -0.7381895106476577 1107 44-45 -0.9445031976675224 1107 46-47 -0.9724470502935318 1107 48-49 -0.8435181099561078 1107 50-51 -0.942867015465481 1107 52-53 -0.801063148257029 1107 54-55 -0.7750593966192056 1107 56-57 -0.6408238949241891 1107 58-59 -0.5616992828219196 1107 60-61 -0.5830513559429917 1107 62-63 -0.7127869527606236 1107 64-65 -0.6306314020914883 1107 66-67 -0.6500886365449787 1107 68-69 -0.6202959108769761 1107 70-71 -0.6449544776161602 1107 72-73 -0.6716204416824922 1107 74-75 -0.6650689801966081 1107 76-77 -0.7067505125076963 1107 78-79 -0.7391901542068915 1107 80-81 -0.739380994525213 1107 82-83 -0.7083497159283887 1107 84-85 -0.7805441672616631 1107 86-87 -0.8033635076652494 1107 88-89 -0.6625781590578264 1107 90-91 -0.7642002825506395 1107 92-93 -1.0064358125978181 1107 94-95 -0.8935825002393791 1107 96-97 -0.8986992338048019 1107 98-99 -0.7104183778778612 1107 100 -0.8139493943303044 1108 1 -0.5554956807103189 1108 2 -0.4784386102419589 1108 3 -0.570589043203551 1108 4 -0.33876724211857834 1108 5 -0.3400789335379031 1108 6 -0.5092255027140737 1108 7 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2207 56-57 1.1317321168662389 2207 58-59 1.2211487204389044 2207 60-61 1.1412464093934815 2207 62-63 1.1303480117477775 2207 64-65 1.1481756987162512 2207 66-67 1.0903300854257836 2207 68-69 1.019320257192625 2207 70-71 0.9829023777996824 2207 72-73 1.0455337590202873 2207 74-75 1.0712001968796727 2207 76-77 0.937617660390913 2207 78-79 0.9623387223835884 2207 80-81 0.9569803652736653 2207 82-83 0.9401888746965206 2207 84-85 0.9265236588108614 2207 86-87 0.9756157615937617 2207 88-89 0.9350510761823578 2207 90-91 0.8949564861586765 2207 92-93 0.8453052408656738 2207 94-95 0.8998910708661896 2207 96-97 0.8704946963238491 2207 98-99 0.8075979874594417 2207 100 0.7842472967165186 2208 1 0.621105205974974 2208 2 0.6777704317686393 2208 3 0.8071751638901326 2208 4 0.5535173447121053 2208 5 0.5828108250855379 2208 6 0.5238936509191845 2208 7 0.628839596559807 2208 8 0.7066296885665864 2208 9 0.8475378690055635 2208 10-11 0.8552278792371695 2208 12-13 0.8120581400404276 2208 14-15 1.071597938427864 2208 16-17 1.0758848235895186 2208 18-19 1.0029456532422998 2208 20-21 1.0500435235064813 2208 22-23 1.0948477898875097 2208 24-25 1.1418345664163425 2208 26-27 1.1326479261871256 2208 28-29 1.0867488134779038 2208 30-31 1.1139900328339465 2208 32-33 1.0815544064592402 2208 34-35 1.147560942212266 2208 36-37 1.2173497499175951 2208 38-39 1.2256198970380368 2208 40-41 1.177507357377479 2208 42-43 1.1608953467122731 2208 44-45 1.1495555793424828 2208 46-47 1.2115098760339862 2208 48-49 1.2651838012664598 2208 50-51 1.2286140417008085 2208 52-53 1.181497497076272 2208 54-55 1.2039881193327062 2208 56-57 1.2579355845112232 2208 58-59 1.1715802760773713 2208 60-61 1.10501758558771 2208 62-63 1.074252255968375 2208 64-65 1.051466472390068 2208 66-67 1.0796642616614704 2208 68-69 1.0155151058156449 2208 70-71 0.9033154690404075 2208 72-73 0.9679953793273199 2208 74-75 0.9824817020821826 2208 76-77 0.9892152021560392 2208 78-79 0.8776028548159864 2208 80-81 0.925983408703857 2208 82-83 0.9813062730300608 2208 84-85 0.9574778657226624 2208 86-87 0.9988206214094255 2208 88-89 0.955973795164244 2208 90-91 0.83818839484886 2208 92-93 0.8463670732801027 2208 94-95 0.8590301704806649 2208 96-97 0.9017537644331206 2208 98-99 0.8478426882291075 2208 100 0.7703722258989032 2209 1 0.6744047488093976 2209 2 0.7287021550840826 2209 3 0.7938974854961778 2209 4 0.6749643731358788 2209 5 0.6805249972183134 2209 6 0.7375514724258423 2209 7 0.7887705248046899 2209 8 0.7608087782107944 2209 9 0.9344365968926596 2209 10-11 0.9611983622425342 2209 12-13 0.9795186349718534 2209 14-15 1.1707531759551983 2209 16-17 1.139073056545186 2209 18-19 1.1094167822424978 2209 20-21 1.0609198403421018 2209 22-23 1.0934899584424755 2209 24-25 1.1828229385093678 2209 26-27 1.2715219075029651 2209 28-29 1.2350617731280735 2209 30-31 1.2511334933785676 2209 32-33 1.3134963663081791 2209 34-35 1.2647170741939817 2209 36-37 1.2953797141394148 2209 38-39 1.29453512263931 2209 40-41 1.3020986414164355 2209 42-43 1.3673341759708677 2209 44-45 1.4877935042083195 2209 46-47 1.3839109891327794 2209 48-49 1.473293521004095 2209 50-51 1.5018313444239197 2209 52-53 1.4037718958041623 2209 54-55 1.2925502839123126 2209 56-57 1.3273340639387712 2209 58-59 1.267168876753182 2209 60-61 1.2624216802011006 2209 62-63 1.3528884614941106 2209 64-65 1.2469407816725138 2209 66-67 1.1726564600315683 2209 68-69 1.237774596970489 2209 70-71 1.231610042683819 2209 72-73 1.1771796866220967 2209 74-75 1.1906957748311413 2209 76-77 1.1473547370397625 2209 78-79 1.217541485306942 2209 80-81 1.2201222479008393 2209 82-83 1.1796068097026868 2209 84-85 1.2917565876467343 2209 86-87 1.364162998670409 2209 88-89 1.362231248950767 2209 90-91 1.4058153576793124 2209 92-93 1.4003126109465711 2209 94-95 1.533653754261227 2209 96-97 1.4274954161922118 2209 98-99 1.4489210026795192 2209 100 1.5631842910946894 2210 1 0.6670636543993389 2210 2 0.6392520617546786 2210 3 0.691549514182686 2210 4 0.59897459024954 2210 5 0.5509788728446026 2210 6 0.5874053382279811 2210 7 0.6275550321236736 2210 8 0.5942600013171884 2210 9 0.7985870537155364 2210 10-11 0.8365311552477479 2210 12-13 0.8763030686097366 2210 14-15 1.0372098514635937 2210 16-17 1.0606296927570042 2210 18-19 1.0248278000242053 2210 20-21 1.0346862957895908 2210 22-23 1.024651541112675 2210 24-25 1.0230049308465325 2210 26-27 1.0286984474381313 2210 28-29 1.055811601205491 2210 30-31 1.1020428410551588 2210 32-33 1.1329555469742587 2210 34-35 1.1197961585834122 2210 36-37 1.1782949071839184 2210 38-39 1.2064702341433886 2210 40-41 1.285214001471445 2210 42-43 1.3129176075456854 2210 44-45 1.293777638209697 2210 46-47 1.3720581548661528 2210 48-49 1.384182360772236 2210 50-51 1.2890888107762137 2210 52-53 1.1662497914717065 2210 54-55 1.17452481480926 2210 56-57 1.1629594937452339 2210 58-59 1.212482144264726 2210 60-61 1.1593442659167934 2210 62-63 1.1375560368944448 2210 64-65 1.109758224446857 2210 66-67 1.0426123741685203 2210 68-69 1.0842170274646392 2210 70-71 1.072350783424561 2210 72-73 1.0118116494868161 2210 74-75 1.0171055400340094 2210 76-77 1.0312308545365667 2210 78-79 1.0765692630847177 2210 80-81 1.013649362548641 2210 82-83 0.9836359383007895 2210 84-85 0.9680699533997519 2210 86-87 0.995644480151892 2210 88-89 1.0411541539512577 2210 90-91 0.8890326432672886 2210 92-93 0.9445360120665249 2210 94-95 0.9703770325793322 2210 96-97 0.896875389667013 2210 98-99 0.8935152653772249 2210 100 1.0139419784903083 2211 1 0.6941780190869942 2211 2 0.7413376264011085 2211 3 0.7561490187851696 2211 4 0.5579294707983777 2211 5 0.6159445888338695 2211 6 0.587674936057951 2211 7 0.6405214915772675 2211 8 0.6580525526879981 2211 9 0.7784583092501123 2211 10-11 0.8635392450228991 2211 12-13 0.8340438387946065 2211 14-15 1.0137120542526858 2211 16-17 1.0291479352367787 2211 18-19 1.054587075845788 2211 20-21 1.0722740301004805 2211 22-23 1.0873178544497648 2211 24-25 1.131826300661146 2211 26-27 1.0894435311882802 2211 28-29 1.100862962812549 2211 30-31 1.1170915526903755 2211 32-33 1.085634226846615 2211 34-35 1.1036225891081415 2211 36-37 1.123813142302673 2211 38-39 1.0760497535415467 2211 40-41 1.1130670458106309 2211 42-43 1.1668912771310005 2211 44-45 1.189069705592587 2211 46-47 1.2185553153477144 2211 48-49 1.2519041394922255 2211 50-51 1.2298324959301112 2211 52-53 0.9951568437092817 2211 54-55 0.9867421051394416 2211 56-57 1.024594618385926 2211 58-59 1.0379999009163328 2211 60-61 0.9971448260913931 2211 62-63 0.9816571811670158 2211 64-65 0.9536606208311156 2211 66-67 0.9792288201882329 2211 68-69 1.0521404064731925 2211 70-71 1.1142309200453298 2211 72-73 1.0381360833313735 2211 74-75 1.0481898017776103 2211 76-77 1.02770215939006 2211 78-79 1.060759144608891 2211 80-81 0.8504718254766459 2211 82-83 0.9051687077789943 2211 84-85 0.9437509409559262 2211 86-87 0.955925726816421 2211 88-89 1.1219483909986003 2211 90-91 0.9483975333486647 2211 92-93 0.9455821988674558 2211 94-95 1.0773471026195622 2211 96-97 1.082882710214232 2211 98-99 1.1279931473136209 2211 100 1.1244053187062093 2212 1 0.6372929769963669 2212 2 0.6483709025669704 2212 3 0.6418766852308373 2212 4 0.4862260505817204 2212 5 0.4866079297872332 2212 6 0.5183561402454444 2212 7 0.5540225720593526 2212 8 0.5861576209295194 2212 9 0.6524055504829605 2212 10-11 0.780348682459227 2212 12-13 0.864657826572774 2212 14-15 0.9923005806601353 2212 16-17 1.0005234683651736 2212 18-19 0.999406210353655 2212 20-21 0.9680454020045488 2212 22-23 0.9027512193302343 2212 24-25 0.9383575345467392 2212 26-27 0.9662547180657768 2212 28-29 1.0339975758019406 2212 30-31 1.0943376246081797 2212 32-33 1.128719688822848 2212 34-35 1.1687355764801168 2212 36-37 1.0346359210523062 2212 38-39 1.1120194079361951 2212 40-41 1.0855499830888249 2212 42-43 1.086832894709275 2212 44-45 1.0652874236447971 2212 46-47 1.167810723009417 2212 48-49 1.1338337237948295 2212 50-51 1.0867348154780672 2212 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2213 78-79 1.1215148399362924 2213 80-81 1.0580848316612617 2213 82-83 1.1185864146733273 2213 84-85 1.0992088763832442 2213 86-87 1.2052059963379875 2213 88-89 1.1656627448202777 2213 90-91 1.3277148081173245 2213 92-93 1.3449375124622556 2213 94-95 1.3097791569743222 2213 96-97 1.4149048788773833 2213 98-99 1.47857979840159 2213 100 1.3984723918490225 2214 1 0.6178174544662447 2214 2 0.6189033622127589 2214 3 0.6300485933619093 2214 4 0.4984094438369553 2214 5 0.5091773791486531 2214 6 0.6244129188504743 2214 7 0.5744508943214512 2214 8 0.5438770300420259 2214 9 0.6907731667304162 2214 10-11 0.7851531538664176 2214 12-13 0.8133696283619329 2214 14-15 0.9152112801470551 2214 16-17 0.9554643731293311 2214 18-19 0.9435385619015833 2214 20-21 0.9152402980165348 2214 22-23 0.9684879142343306 2214 24-25 1.0562148597988 2214 26-27 1.0296724981364989 2214 28-29 1.014027061523059 2214 30-31 1.1269509389360692 2214 32-33 1.1291812425908034 2214 34-35 1.1697287119184594 2214 36-37 1.145433766805823 2214 38-39 1.133921879213105 2214 40-41 1.193400535779361 2214 42-43 1.2333703232755582 2214 44-45 1.2319821532434503 2214 46-47 1.2550325082741693 2214 48-49 1.1006880969117177 2214 50-51 0.9658822328462833 2214 52-53 0.9243091517352084 2214 54-55 0.9753076734870376 2214 56-57 0.8378870745344287 2214 58-59 0.9108084895136059 2214 60-61 0.8740242252877763 2214 62-63 0.8498549992330524 2214 64-65 0.9586089791183028 2214 66-67 0.9711629045165822 2214 68-69 0.99955351443392 2214 70-71 1.041476056725152 2214 72-73 1.00180873415421 2214 74-75 0.9907961787286297 2214 76-77 1.0267253491232111 2214 78-79 1.0661577465548469 2214 80-81 1.109375132611305 2214 82-83 1.042692118786963 2214 84-85 1.0365177575803308 2214 86-87 1.037140848962114 2214 88-89 1.184712736982167 2214 90-91 1.276577524045532 2214 92-93 1.3264579379818429 2214 94-95 1.3293658137581446 2214 96-97 1.3735949244413064 2214 98-99 1.3914523218498154 2214 100 1.3187474846992657 2215 1 0.5675829359246656 2215 2 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2310 78-79 1.0615168161394983 2310 80-81 1.0761144049050273 2310 82-83 1.0302846056485429 2310 84-85 1.1920473936678455 2310 86-87 1.1514845574908712 2310 88-89 1.2301727030109326 2310 90-91 1.4176676692991315 2310 92-93 1.3846594821643414 2310 94-95 1.3381647292453955 2310 96-97 1.5209295121837485 2310 98-99 1.4481022371999188 2310 100 1.6267216745719004 2311 1 0.6634093728641197 2311 2 0.6819564540099528 2311 3 0.7666854051532219 2311 4 0.5795771888319763 2311 5 0.641946251814268 2311 6 0.6403100502079013 2311 7 0.7247287718383859 2311 8 0.75150286809086 2311 9 0.877048344881679 2311 10-11 0.862553266470016 2311 12-13 0.9853612204285582 2311 14-15 1.1408515575360454 2311 16-17 1.1306285371739335 2311 18-19 1.1576577749692447 2311 20-21 1.1408588887180642 2311 22-23 1.1856538514790884 2311 24-25 1.2044859524939255 2311 26-27 1.2599192229823117 2311 28-29 1.313947865641282 2311 30-31 1.370747292489078 2311 32-33 1.3500862062323904 2311 34-35 1.3312733576100015 2311 36-37 1.3203872402701435 2311 38-39 1.3665549755686826 2311 40-41 1.4924472317219752 2311 42-43 1.511797251770048 2311 44-45 1.510370491508219 2311 46-47 1.4659369833848217 2311 48-49 1.5719129275837318 2311 50-51 1.4703684050985828 2311 52-53 1.364765866871707 2311 54-55 1.3174008651303097 2311 56-57 1.3437511641105644 2311 58-59 1.330317629221753 2311 60-61 1.3683482572767076 2311 62-63 1.2941020550910807 2311 64-65 1.2607028636210416 2311 66-67 1.2674159650329386 2311 68-69 1.3397958146382294 2311 70-71 1.3369586129196804 2311 72-73 1.2778627582467763 2311 74-75 1.3385860498660591 2311 76-77 1.2706118858604611 2311 78-79 1.2286338981643858 2311 80-81 1.3511020096335784 2311 82-83 1.261980611542679 2311 84-85 1.1789094824976551 2311 86-87 1.2519786947672564 2311 88-89 1.2433782914824398 2311 90-91 1.2955383759989196 2311 92-93 1.346398220181836 2311 94-95 1.4760582827842583 2311 96-97 1.4342596014434044 2311 98-99 1.4415562321963975 2311 100 1.5826271968635197 2312 1 0.5672672819019091 2312 2 0.6547162704315888 2312 3 0.6785091588019618 2312 4 0.47077224276169716 2312 5 0.5157915104841777 2312 6 0.3506863125508701 2312 7 0.5002317704851365 2312 8 0.5915136796136693 2312 9 0.803453656545777 2312 10-11 0.6981859953342209 2312 12-13 0.8380471728808203 2312 14-15 0.9460114212480164 2312 16-17 1.0112996773565683 2312 18-19 0.9653259603111266 2312 20-21 0.9814140527609752 2312 22-23 0.9557747540317862 2312 24-25 0.9941114021096737 2312 26-27 1.1066177005273587 2312 28-29 1.0928543892714444 2312 30-31 1.0330829721457562 2312 32-33 1.1738210234244093 2312 34-35 1.1578851717766838 2312 36-37 1.0846288234251134 2312 38-39 1.175539506108315 2312 40-41 1.1955587469001046 2312 42-43 1.2774140412915642 2312 44-45 1.2509605090165294 2312 46-47 1.1060042089825117 2312 48-49 1.210067341426189 2312 50-51 1.190957155757431 2312 52-53 1.056195277879361 2312 54-55 1.0795587931204835 2312 56-57 1.1021821579751077 2312 58-59 1.0937092248740186 2312 60-61 1.1027300075551025 2312 62-63 1.0347162917800503 2312 64-65 1.1018301743373158 2312 66-67 1.1367199007454687 2312 68-69 1.1262265478822329 2312 70-71 1.123357121058426 2312 72-73 1.0974182310464755 2312 74-75 1.1473278850817792 2312 76-77 1.1292272576787639 2312 78-79 1.1843522229503414 2312 80-81 0.976110342844386 2312 82-83 1.1101066612503097 2312 84-85 1.1800419935402893 2312 86-87 1.3552828657230513 2312 88-89 1.3018084998600372 2312 90-91 1.259591976016484 2312 92-93 1.2474074802672384 2312 94-95 1.3776552985783894 2312 96-97 1.4018151640705803 2312 98-99 1.1271492234776943 2312 100 1.3642322205963033 2313 1 0.7379002167944684 2313 2 0.7530532529465255 2313 3 0.7690101799850488 2313 4 0.6680330561856564 2313 5 0.5956721059381991 2313 6 0.6615717574338404 2313 7 0.681123658438537 2313 8 0.7200594204482016 2313 9 0.8237990853808626 2313 10-11 0.8048311236210424 2313 12-13 0.9551896518881406 2313 14-15 1.1888975945485711 2313 16-17 1.1509411191694028 2313 18-19 1.0704447862171023 2313 20-21 1.1493774405553765 2313 22-23 1.2184242199906237 2313 24-25 1.2522227494734537 2313 26-27 1.299450752445047 2313 28-29 1.297490278774923 2313 30-31 1.3146074524692537 2313 32-33 1.3492423311184183 2313 34-35 1.3548981124374038 2313 36-37 1.3528960124283955 2313 38-39 1.3651285257705226 2313 40-41 1.411511963601498 2313 42-43 1.4841592813947457 2313 44-45 1.3285434678552548 2313 46-47 1.417526664572648 2313 48-49 1.5082076066034347 2313 50-51 1.43294562632979 2313 52-53 1.2637635346001446 2313 54-55 1.256570144801266 2313 56-57 1.2077073492860606 2313 58-59 1.2323532473605212 2313 60-61 1.2498907800993138 2313 62-63 1.2647047210065026 2313 64-65 1.233251127753654 2313 66-67 1.2231160233101335 2313 68-69 1.1964260195869443 2313 70-71 1.2466226450782685 2313 72-73 1.2656494045223425 2313 74-75 1.3221290548466484 2313 76-77 1.3376036343302502 2313 78-79 1.3779502754770725 2313 80-81 1.3653557557288636 2313 82-83 1.3430398715998386 2313 84-85 1.3699577776035916 2313 86-87 1.3877452292942287 2313 88-89 1.437921048281872 2313 90-91 1.5497077405012796 2313 92-93 1.5577746825863379 2313 94-95 1.701951764537025 2313 96-97 1.6603061742747123 2313 98-99 1.7644098301721236 2313 100 1.7249608651822292 2314 1 0.4625288314830591 2314 2 0.4841462613375427 2314 3 0.5840745745941298 2314 4 0.4819188095867162 2314 5 0.44846208567463464 2314 6 0.502752121271314 2314 7 0.6212600181598376 2314 8 0.6257975898370773 2314 9 0.7097202161511049 2314 10-11 0.716105506761032 2314 12-13 0.4960477026875836 2314 14-15 0.8709579953772462 2314 16-17 0.8975128598864686 2314 18-19 0.8445899313486294 2314 20-21 0.9247873583678157 2314 22-23 0.7419403787082643 2314 24-25 0.7664729750019106 2314 26-27 0.966332747793949 2314 28-29 1.012152575402645 2314 30-31 0.9503619868379829 2314 32-33 0.9878018049004496 2314 34-35 1.0243300527074908 2314 36-37 1.118830334913703 2314 38-39 1.0811167937572677 2314 40-41 1.1089887647692578 2314 42-43 1.1561774324693488 2314 44-45 1.181630374511407 2314 46-47 1.096793459076558 2314 48-49 1.1032876862070395 2314 50-51 1.1191460044016281 2314 52-53 1.0347728141330421 2314 54-55 0.9742311766563176 2314 56-57 1.0168808044727697 2314 58-59 0.9815893971930194 2314 60-61 0.9738292096959 2314 62-63 1.0375556274269186 2314 64-65 1.0444203398320084 2314 66-67 1.069462008903514 2314 68-69 1.1247374347270451 2314 70-71 1.1857571597727556 2314 72-73 1.0773512717620122 2314 74-75 1.1047762829998504 2314 76-77 1.1781674781512166 2314 78-79 1.2054357351244782 2314 80-81 1.2074325668697412 2314 82-83 1.1178400692488957 2314 84-85 1.1318393876274833 2314 86-87 1.3501681076267218 2314 88-89 1.5039204226953835 2314 90-91 1.5078671109255453 2314 92-93 1.6005548252019217 2314 94-95 1.5766610708160087 2314 96-97 1.6339012112885776 2314 98-99 1.6831153194668893 2314 100 1.5674989330266307 2315 1 0.41853144292106137 2315 2 0.4376497243518571 2315 3 0.528711769228078 2315 4 0.43243079927337646 2315 5 0.38427015965258704 2315 6 -0.2730990741612658 2315 7 0.26479412287428516 2315 8 0.39470488811411997 2315 9 0.7024502654249716 2315 10-11 0.7457422433964567 2315 12-13 0.762494740095427 2315 14-15 0.8993038721021023 2315 16-17 0.7543759536881396 2315 18-19 0.8222316094778037 2315 20-21 0.9068390356487086 2315 22-23 0.7520138070632783 2315 24-25 0.8561968611391606 2315 26-27 0.8876335068677221 2315 28-29 0.9782774244841406 2315 30-31 0.97424693911303 2315 32-33 1.0533868176087324 2315 34-35 0.9193368530921688 2315 36-37 1.1252258564216646 2315 38-39 1.199682408206023 2315 40-41 1.1559061525563266 2315 42-43 1.1674410456996611 2315 44-45 1.1822842685051569 2315 46-47 1.217085126080221 2315 48-49 1.3241452023954565 2315 50-51 1.24870494711908 2315 52-53 1.0484812178511262 2315 54-55 0.8864045517631212 2315 56-57 0.9344908665969243 2315 58-59 1.0119463294568192 2315 60-61 0.9221049063756084 2315 62-63 0.9779947530277937 2315 64-65 1.0339950896115155 2315 66-67 1.1279730603985385 2315 68-69 1.1224680379252092 2315 70-71 1.2056579480756824 2315 72-73 1.18825067741723 2315 74-75 1.073236595405632 2315 76-77 1.0365559797737731 2315 78-79 1.0549299900173033 2315 80-81 1.142685540991792 2315 82-83 1.2128339478681873 2315 84-85 1.2105522650601195 2315 86-87 1.2190888355114602 2315 88-89 1.3727951626378037 2315 90-91 1.4117014802258403 2315 92-93 1.468827960099322 2315 94-95 1.6235606841223031 2315 96-97 1.6280918244442155 2315 98-99 1.69054411198152 2315 100 1.6788200358870817 2316 1 0.06222988997389223 2316 2 -0.01247737837884344 2316 3 0.1442538679825205 2316 4 0.07448302536211315 2316 5 0.07957618670271671 2316 6 0.19622151778361285 2316 7 0.13691803012728343 2316 8 0.16341916421468028 2316 9 0.43047357736104885 2316 10-11 0.2055483113307517 2316 12-13 0.34621249315939195 2316 14-15 0.5504521021793991 2316 16-17 0.5871360945901785 2316 18-19 0.5723061416796895 2316 20-21 0.4889346279011164 2316 22-23 0.3652310257385949 2316 24-25 0.4778439974744728 2316 26-27 0.6026313215546182 2316 28-29 0.6228487304547414 2316 30-31 0.669700673791418 2316 32-33 0.713251064077312 2316 34-35 0.7488719385834983 2316 36-37 0.8483088773348229 2316 38-39 0.8554776848905377 2316 40-41 0.9128068937537535 2316 42-43 0.9243871936511425 2316 44-45 0.7836175808536936 2316 46-47 0.6763019916320872 2316 48-49 0.8383252251603466 2316 50-51 0.6216377639370165 2316 52-53 0.4443474303778885 2316 54-55 0.33552619338190937 2316 56-57 0.47370602080260227 2316 58-59 0.5480833218147367 2316 60-61 0.5558170333437502 2316 62-63 0.5741099971786454 2316 64-65 0.5653378704441536 2316 66-67 0.5211620834695125 2316 68-69 0.5244715792902142 2316 70-71 0.39599030742509456 2316 72-73 0.5723795153733704 2316 74-75 0.6733556841155917 2316 76-77 0.7938950499158111 2316 78-79 0.7957830617209005 2316 80-81 0.8079855729031493 2316 82-83 0.8985378936608548 2316 84-85 0.9423669505089016 2316 86-87 1.1338982573948364 2316 88-89 1.2107785274845106 2316 90-91 1.2421333943357986 2316 92-93 1.2502316149269497 2316 94-95 1.3838024398115358 2316 96-97 1.4164145821646308 2316 98-99 1.5417239277929085 2316 100 1.437474997379983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 7.0 1 138.0 2 75.0 3 64.0 4 60.0 5 38.0 6 46.0 7 208.0 8 701.0 9 1416.0 10 2063.0 11 2674.0 12 3299.0 13 4026.0 14 4856.0 15 6250.0 16 8218.0 17 11385.0 18 14686.0 19 17860.0 20 22110.0 21 26614.0 22 28591.0 23 29207.0 24 29116.0 25 29749.0 26 33129.0 27 38299.0 28 45190.0 29 54706.0 30 65660.0 31 81595.0 32 102324.0 33 131905.0 34 180088.0 35 242129.0 36 344714.0 37 509569.0 38 495575.0 39 133975.0 40 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12692049529588 18.96160678194892 12.529250121450582 23.38222260130462 2 16.316019817016112 20.893322151767155 40.37396483735898 22.416693193857746 3 18.820218377186798 27.01685777723987 30.64364679020475 23.519277055368583 4 12.752149810538347 17.187343857911532 38.396167685592104 31.66433864595802 5 12.763402764720427 37.636448295108806 34.59028128372806 15.0098676564427 6 29.00786307774443 36.93932345987268 19.634369476537756 14.418443985845128 7 25.99972008356024 33.624888274092996 23.12754044533373 17.247851197013038 8 25.484789157072573 35.06698339262072 21.366413819045615 18.08181363126109 9 27.47395332032233 16.00819456084913 21.00037611464685 35.51747600418169 10-11 24.437609775140164 25.91891822354786 29.493638247773585 20.149833753538392 12-13 25.011674871622684 24.408468543740465 28.590987832035108 21.988868752601743 14-15 22.837539927499932 25.897798553884755 26.913776585871908 24.35088493274341 16-17 21.779235388481773 28.075584389485293 27.749046858672315 22.396133363360622 18-19 21.284665081779828 27.6869503684427 29.675306687076386 21.35307786270108 20-21 22.406593469634444 27.1787794116368 29.244818109427655 21.169809009301098 22-23 22.19414409796183 26.902694401890304 29.17491275218957 21.728248747958297 24-25 21.715528550535744 26.543251571264427 29.574987383805635 22.16623249439419 26-27 21.62804740050212 26.790477041711842 29.390557835078273 22.190917722707766 28-29 21.474331928425187 27.847862807536465 29.173550568551075 21.50425469548727 30-31 22.677206472735765 26.95711212077081 29.348146500540985 21.017534905952438 32-33 22.36038730309132 26.954078082435153 29.09218435390264 21.593350260570887 34-35 22.39869981025328 27.169498247345853 29.20774406456237 21.224057877838497 36-37 21.976075984786462 27.03303052330961 29.207244492938795 21.783648998965134 38-39 21.926680359730696 27.188270130756486 29.186323689895215 21.6987258196176 40-41 21.94710304428239 26.540193847182213 29.678616517242645 21.834086591292756 42-43 21.65264869518676 26.870735709386594 29.037318291666836 22.439297303759812 44-45 20.993549373455757 27.22328762990421 29.562530505405505 22.220632491234525 46-47 20.90510703004467 26.564311965578614 29.32418687430067 23.20639413007605 48-49 21.160960163001008 26.690748604485265 29.422788675072642 22.725502557441086 50-51 21.16608884921824 27.037303879664925 29.232319258005035 22.564288013111796 52-53 20.8744773777784 27.342795013626862 28.835475155870434 22.9472524527243 54-55 20.62829165218312 26.886331640470416 29.373355677791142 23.112021029555322 56-57 21.220181441656358 26.772602650472706 28.777208659385607 23.230007248485332 58-59 20.861936647523134 26.791212558395927 29.1513168538261 23.195533940254844 60-61 21.448565909534214 26.74762946565219 28.85350858329783 22.950296041515767 62-63 21.466816000094752 26.533634813145685 28.794104191896906 23.205444994862653 64-65 21.099457633028145 26.315133957628124 28.724925914266898 23.860482495076834 66-67 21.163670403875017 26.369833160936484 28.36909989596965 24.09739653921885 68-69 20.816123771146568 26.616230526573737 28.5873562188738 23.980289483405897 70-71 21.130571571407234 26.49861451649115 28.574146735206345 23.79666717689527 72-73 21.308448960425096 26.979897424241216 28.0702715579288 23.64138205740489 74-75 21.773458981365614 27.034072794284477 27.786432396080734 23.40603582826918 76-77 21.719081556197512 26.859397920083524 27.731331445954428 23.69018907776454 78-79 21.688946376956412 27.005488506704268 27.903190587747638 23.402374528591675 80-81 21.955595346131126 27.031015676236635 27.631051058430955 23.38233791920128 82-83 22.48250177276879 26.884760406041774 27.692387975076127 22.940349846113307 84-85 22.726609119080056 27.17964213569874 27.064882495343095 23.028866249878106 86-87 22.456557890153714 27.263968191025562 27.332784260419697 22.946689658401027 88-89 22.652119467935076 26.59995221993191 27.389544677417 23.358383634716013 90-91 23.404755353042965 26.364383132221995 27.278447059145737 22.9524144555893 92-93 23.643257001128262 26.427738296878967 27.095610393941094 22.83339430805168 94-95 23.489317964961472 26.325659017272024 26.973776253541125 23.21124676422538 96-97 23.71992211854437 26.100984214773977 26.86310618649022 23.315987480191435 98-99 23.785582580754653 26.141711998439476 26.562657600127032 23.51004782067884 100 24.312627997970168 26.171381532314985 26.352230426512463 23.16376004320238 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1545.0 1 1077.5 2 1321.5 3 1945.5 4 1353.5 5 938.0 6 1237.5 7 1875.5 8 2861.0 9 3440.0 10 2990.0 11 2479.5 12 2538.5 13 2732.5 14 2976.0 15 3307.0 16 3581.0 17 3865.5 18 4194.5 19 4626.5 20 5103.0 21 5726.0 22 6468.5 23 7463.0 24 8809.5 25 10482.0 26 12753.5 27 15399.5 28 18362.0 29 22398.5 30 27481.0 31 32360.0 32 38269.0 33 44666.0 34 50522.5 35 56369.5 36 63195.0 37 70916.0 38 75785.5 39 78376.0 40 80330.0 41 81949.5 42 83929.0 43 87624.0 44 92737.0 45 98391.5 46 104905.0 47 111606.5 48 118246.0 49 122039.0 50 122492.5 51 120559.5 52 115065.5 53 107651.0 54 98743.5 55 87170.0 56 75903.0 57 64349.0 58 52625.5 59 42416.5 60 32492.5 61 24514.5 62 19079.5 63 14772.0 64 10832.0 65 7624.0 66 5423.5 67 4109.5 68 3224.0 69 2507.5 70 1985.5 71 1524.0 72 1222.5 73 976.0 74 692.0 75 497.0 76 375.0 77 258.5 78 168.5 79 107.0 80 78.0 81 65.0 82 45.0 83 31.0 84 28.5 85 24.0 86 16.5 87 14.5 88 13.0 89 7.5 90 7.0 91 5.5 92 5.5 93 3.5 94 1.5 95 2.0 96 2.5 97 2.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.219168005245527 2 0.14815949569527098 3 0.0241998776685083 4 0.023200800150083644 5 0.027271115965887795 6 0.05605934964493895 7 0.062423843466014525 8 0.0564293783554666 9 0.04399641368173757 10-11 0.04560603857253284 12-13 0.04360788353568354 14-15 0.020888120709285835 16-17 0.018741954188225465 18-19 0.018963971414542057 20-21 0.022479244164554726 22-23 0.026124026963252077 24-25 0.022516247035607492 26-27 0.019926046061913946 28-29 0.019870541755334797 30-31 0.017853885282959104 32-33 0.027123104481676735 34-35 0.027141605917203118 36-37 0.025494978155355074 38-39 0.02362633316719044 40-41 0.0249399350895636 42-43 0.031156417426428117 44-45 0.04172073711199252 46-47 0.04682713331727409 48-49 0.042923330421207384 50-51 0.02973180689089667 52-53 0.044810476844898396 54-55 0.04262730745278526 56-57 0.045846557234375813 58-59 0.04884378978964978 60-61 0.02501394083166913 62-63 0.026235035576410375 64-65 0.026808580077728232 66-67 0.032470019348801275 68-69 0.0366698452132901 70-71 0.04031462801198745 72-73 0.043385866309366944 74-75 0.02442189489482489 76-77 0.01885296280138376 78-79 0.017872386718485487 80-81 0.021054633629023278 82-83 0.018686449881646316 84-85 0.011970428785569472 86-87 0.013413540756627306 88-89 0.01959302022243906 90-91 0.020296074772441594 92-93 0.020481089127705417 94-95 0.024680914992194245 96-97 0.025698493946145283 98-99 0.030989904506690674 100 0.029232268131684336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2702493.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.24134371522798 #Duplication Level Percentage of deduplicated Percentage of total 1 84.47995124741851 61.02945195110463 2 10.35223613998737 14.957188984200654 3 2.5285420898839384 5.479958346411794 4 0.9637542139941307 2.784915977205975 5 0.4863927525636598 1.7568833009273592 6 0.28190156234977126 1.221896859574176 7 0.20006887992745173 1.0117271305091802 8 0.11961060406184716 0.6912664608014356 9 0.10310671112574951 0.6703710622003792 >10 0.44591021221653754 5.556597720081831 >50 0.024323029397395605 1.2190189492601924 >100 0.012792027257988689 1.6575689378555671 >500 8.137672014336027E-4 0.3583694296781133 >1k 4.340091740574922E-4 0.6042977073245709 >5k 1.0850229351437306E-4 0.5631463620563434 >10k+ 5.425114675718653E-5 0.43734082080776226 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11159 0.41291503807780444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9081 0.33602307203015885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5288 0.19567118212702123 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3007 0.1112676332556643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2712 0.10035178629509862 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.700287105276498E-5 0.0 6 0.0 0.0 0.0 3.700287105276498E-5 0.0 7 0.0 0.0 0.0 3.700287105276498E-5 0.0 8 0.0 0.0 0.0 3.700287105276498E-5 0.0 9 0.0 0.0 0.0 7.400574210552996E-5 0.0 10-11 0.0 0.0 0.0 1.8501435526382492E-4 0.0 12-13 0.0 0.0 0.0 2.220172263165899E-4 0.0 14-15 0.0 1.850143552638249E-5 0.0 2.220172263165899E-4 1.850143552638249E-5 16-17 0.0 3.700287105276498E-5 0.0 2.5902009736935487E-4 3.700287105276498E-5 18-19 0.0 3.700287105276498E-5 0.0 4.625358881595623E-4 3.700287105276498E-5 20-21 0.0 3.700287105276498E-5 0.0 9.990775184246545E-4 3.700287105276498E-5 22-23 0.0 3.700287105276498E-5 0.0 0.003718788540802881 3.700287105276498E-5 24-25 0.0 5.5504306579147474E-5 0.0 0.010601322556617169 7.400574210552996E-5 26-27 0.0 7.400574210552996E-5 0.0 0.021942702534289635 7.400574210552996E-5 28-29 0.0 7.400574210552996E-5 0.0 0.057613470229155084 7.400574210552996E-5 30-31 0.0 7.400574210552996E-5 0.0 0.16161003932295107 9.250717763191246E-5 32-33 0.0 7.400574210552996E-5 0.0 0.38714253838955365 1.4801148421105992E-4 34-35 0.0 7.400574210552996E-5 0.0 0.7169676295183743 1.4801148421105992E-4 36-37 0.0 7.400574210552996E-5 0.0 1.1953222450529937 1.4801148421105992E-4 38-39 0.0 7.400574210552996E-5 0.0 1.8712166877027987 1.8501435526382492E-4 40-41 0.0 7.400574210552996E-5 0.0 2.606075205375185 2.220172263165899E-4 42-43 0.0 7.400574210552996E-5 0.0 3.2808595618934073 2.4051866184297238E-4 44-45 0.0 7.400574210552996E-5 0.0 4.055218644414619 2.5902009736935487E-4 46-47 0.0 7.400574210552996E-5 0.0 4.93546144245332 2.5902009736935487E-4 48-49 0.0 7.400574210552996E-5 0.0 5.80552845095251 2.5902009736935487E-4 50-51 0.0 7.400574210552996E-5 0.0 6.6736158058503765 2.5902009736935487E-4 52-53 0.0 7.400574210552996E-5 0.0 7.503275679159946 2.5902009736935487E-4 54-55 0.0 7.400574210552996E-5 0.0 8.414360370221125 2.5902009736935487E-4 56-57 0.0 7.400574210552996E-5 0.0 9.392179739225966 2.5902009736935487E-4 58-59 0.0 7.400574210552996E-5 0.0 10.438047388096843 2.5902009736935487E-4 60-61 0.0 7.400574210552996E-5 0.0 11.450575450149177 2.5902009736935487E-4 62-63 0.0 7.400574210552996E-5 0.0 12.467284836630474 2.5902009736935487E-4 64-65 0.0 7.400574210552996E-5 0.0 13.456057055466934 2.5902009736935487E-4 66-67 0.0 7.400574210552996E-5 0.0 14.519908099669454 2.775215328957374E-4 68-69 0.0 7.400574210552996E-5 0.0 15.671622461186764 2.9602296842211985E-4 70-71 0.0 7.400574210552996E-5 0.0 16.82744414139093 2.9602296842211985E-4 72-73 0.0 7.400574210552996E-5 0.0 17.880157321406568 3.330258394748849E-4 74-75 0.0 7.400574210552996E-5 0.0 18.906653967281322 3.330258394748849E-4 76-77 0.0 1.1100861315829495E-4 0.0 19.994205350393138 3.330258394748849E-4 78-79 0.0 1.1100861315829495E-4 0.0 21.147418328188085 3.330258394748849E-4 80-81 0.0 1.1100861315829495E-4 0.0 22.2547477458776 3.330258394748849E-4 82-83 0.0 1.1100861315829495E-4 0.0 23.348756129988125 3.330258394748849E-4 84-85 0.0 1.4801148421105995E-4 0.0 24.419822734045937 3.330258394748849E-4 86-87 0.0 1.8501435526382492E-4 0.0 25.480140004062918 3.5152727500126733E-4 88 0.0 1.8501435526382492E-4 0.0 26.291501957636893 3.7002871052764985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5660 0.0 48.357143 1 TACCTGG 2210 0.0 43.013924 2 GTATCAA 9845 0.0 39.237297 1 ACCTGGG 2635 0.0 35.857063 3 ATCAACG 11500 0.0 32.945526 3 CAACGCA 11590 0.0 32.933044 5 TATCAAC 11770 0.0 32.306126 2 TCAACGC 11960 0.0 31.795713 4 AACGCAG 12305 0.0 31.211004 6 GTACATG 9200 0.0 28.418825 1 ACGCAGA 13400 0.0 28.380436 7 CGCAGAG 13585 0.0 27.855541 8 TACATGG 9305 0.0 27.76553 2 ATAACGC 305 0.0 27.741627 3 GTACACG 340 0.0 27.710989 1 ACATGGG 9405 0.0 26.639599 3 CATGGGG 7020 0.0 26.38216 4 ATGGGGG 5565 0.0 26.185196 5 CCTGGGG 2620 0.0 25.655825 4 TAACGCA 275 0.0 25.639515 4 >>END_MODULE