FastQCFastQC Report
Mon 6 Jun 2016
ERR1211388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702493
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.36914064162238347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85950.3180396766985151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.19234092373227238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27710.10253495568721177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.049.6569481
TACCTGG22850.046.27492
GTATCAA90200.039.412841
ACCTGGG26350.038.523193
ATCAACG108600.032.022193
CAACGCA109850.031.9964985
TATCAAC113150.031.399042
TCAACGC112350.031.2473164
AACGCAG117050.030.3873796
CCTGGGG26350.028.35844
TAACGCA2900.027.5496524
ACGCAGA131150.026.9054577
GTACATG88300.026.5743271
CGCAGAG133800.026.3374618
TATTCGC1458.680199E-725.9233269
TACATGG90700.025.6476152
ATAACGC3150.025.3622343
TGGGGGG57900.024.9942976
ATGGGGG52400.024.570855
GTACCTG44000.024.260271
Produced by FastQC (version 0.11.5)