##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211388_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2702493 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.105505176146615 33.0 31.0 34.0 30.0 34.0 2 32.23627998296388 33.0 31.0 34.0 30.0 34.0 3 32.31166963244678 34.0 31.0 34.0 30.0 34.0 4 35.94741003954497 37.0 35.0 37.0 35.0 37.0 5 35.811940308448534 37.0 35.0 37.0 35.0 37.0 6 35.847363896964765 37.0 35.0 37.0 35.0 37.0 7 35.78626808654084 37.0 35.0 37.0 33.0 37.0 8 35.770305417997385 37.0 35.0 37.0 33.0 37.0 9 37.36355468820826 39.0 37.0 39.0 34.0 39.0 10-11 37.346623099486294 39.0 37.0 39.0 34.0 39.0 12-13 37.26141825344229 39.0 37.0 39.0 33.5 39.0 14-15 38.47636737634473 40.0 38.0 41.0 34.0 41.0 16-17 38.45502227017795 40.0 38.0 41.0 34.0 41.0 18-19 38.46483099123661 40.0 38.0 41.0 34.0 41.0 20-21 38.42347399234707 40.0 38.0 41.0 34.0 41.0 22-23 38.32324912589968 40.0 38.0 41.0 34.0 41.0 24-25 38.20434761533147 40.0 37.5 41.0 33.5 41.0 26-27 38.07932823507776 40.0 37.0 41.0 33.0 41.0 28-29 37.986975914461205 40.0 37.0 41.0 33.0 41.0 30-31 38.0254422490641 40.0 37.0 41.0 33.0 41.0 32-33 37.84874188388277 40.0 37.0 41.0 33.0 41.0 34-35 37.73544834343697 40.0 37.0 41.0 33.0 41.0 36-37 37.58707108584555 40.0 37.0 41.0 33.0 41.0 38-39 37.4715072342463 40.0 36.0 41.0 32.0 41.0 40-41 37.3980539450056 40.0 36.0 41.0 32.0 41.0 42-43 37.25874646113792 40.0 36.0 41.0 31.5 41.0 44-45 37.107549584772286 40.0 36.0 41.0 31.0 41.0 46-47 36.89224745447999 39.5 36.0 41.0 31.0 41.0 48-49 36.67410017343246 39.0 35.0 41.0 30.0 41.0 50-51 36.497504526376204 39.0 35.0 41.0 30.0 41.0 52-53 36.30239042247288 39.0 35.0 40.5 29.0 41.0 54-55 36.10415364628142 39.0 35.0 40.0 29.0 41.0 56-57 35.884604881492756 38.0 35.0 40.0 28.0 41.0 58-59 35.631457139759476 38.0 34.5 40.0 28.0 41.0 60-61 35.368161360639974 38.0 34.0 40.0 27.5 41.0 62-63 35.068590001898244 37.0 34.0 40.0 27.0 41.0 64-65 34.721740814869825 37.0 34.0 40.0 26.0 41.0 66-67 34.3685929991308 36.0 33.5 39.0 26.0 41.0 68-69 33.98963420071763 36.0 33.0 39.0 26.0 40.0 70-71 33.601030048921494 35.0 33.0 38.5 25.0 40.0 72-73 33.23577174852997 35.0 32.0 37.5 25.0 40.0 74-75 32.85459610811203 35.0 32.0 37.0 24.0 39.0 76-77 31.800228899760334 34.0 30.5 36.0 23.5 38.5 78-79 32.26089688298916 35.0 31.5 36.0 24.0 38.5 80-81 32.14253986966848 35.0 32.0 36.0 24.0 37.5 82-83 31.86315431714347 34.5 31.0 35.0 23.5 37.0 84-85 31.580848868063672 34.0 31.0 35.0 23.0 36.5 86-87 31.315127920775375 34.0 31.0 35.0 21.5 36.0 88-89 31.085136390732558 34.0 31.0 35.0 20.5 36.0 90-91 30.86542647844046 34.0 30.5 35.0 20.0 35.0 92-93 30.682934053853238 34.0 30.0 35.0 20.0 35.0 94-95 30.516584131762784 34.0 30.0 35.0 19.0 35.0 96-97 30.342461016550274 34.0 30.0 35.0 19.0 35.0 98-99 30.142151154508078 34.0 29.0 35.0 18.0 35.0 100 28.233404859883077 32.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22712277522538216 1101 2 -0.25991318872023683 1101 3 -0.20756927281079385 1101 4 -0.14482000315318544 1101 5 -0.16317420694270623 1101 6 -0.18780802042280698 1101 7 -0.24998756249486576 1101 8 -0.22262174152828607 1101 9 -0.4617905966981368 1101 10-11 -0.3106753042779147 1101 12-13 -0.48717524025256154 1101 14-15 -0.5047734403554571 1101 16-17 -0.6190727051357854 1101 18-19 -0.6135344216973735 1101 20-21 -0.5755870960231064 1101 22-23 -0.6170479596838803 1101 24-25 -0.5604207921695945 1101 26-27 -0.6129871768123891 1101 28-29 -0.7250512269578167 1101 30-31 -0.5705775790846417 1101 32-33 -0.5129032238588067 1101 34-35 -0.4103821035122337 1101 36-37 -0.4935596434991183 1101 38-39 -0.5996626423922109 1101 40-41 -0.6967303030037684 1101 42-43 -0.5580746273203232 1101 44-45 -0.5716407859395858 1101 46-47 -0.6855289365917585 1101 48-49 -0.605531064785815 1101 50-51 -0.6855817542727465 1101 52-53 -0.7906612816224197 1101 54-55 -0.7449893222481236 1101 56-57 -0.6196162323038479 1101 58-59 -0.6426153858389441 1101 60-61 -0.5631107610093622 1101 62-63 -0.7305475637056205 1101 64-65 -0.6818023279987244 1101 66-67 -0.707483550876546 1101 68-69 -0.8329763194519657 1101 70-71 -0.7891718182276861 1101 72-73 -0.7846574504464101 1101 74-75 -0.7662200238293551 1101 76-77 -0.7477819035206892 1101 78-79 -0.7712803122188703 1101 80-81 -0.6127385241942456 1101 82-83 -0.8149148040699821 1101 84-85 -0.7792509403150234 1101 86-87 -1.0499717564658546 1101 88-89 -1.170991522435461 1101 90-91 -1.1412696603829495 1101 92-93 -1.3237903296077178 1101 94-95 -1.2891162045379723 1101 96-97 -1.3487706500768617 1101 98-99 -1.4716683356543676 1101 100 -1.4699547893723874 1102 1 -0.2682638103560535 1102 2 -0.1662609490280289 1102 3 -0.15542848983358937 1102 4 -0.1498941444079378 1102 5 -0.20086090218676134 1102 6 -0.29813915486941056 1102 7 -0.27108044444637613 1102 8 -0.2398295078552266 1102 9 -0.22757564806424568 1102 10-11 -0.29814953988687165 1102 12-13 -0.3372373025426043 1102 14-15 -0.4439697214984477 1102 16-17 -0.5257878774542135 1102 18-19 -0.49644354070567687 1102 20-21 -0.543342604763076 1102 22-23 -0.42409727602261427 1102 24-25 -0.5824080868882007 1102 26-27 -0.6935470388600393 1102 28-29 -0.6546611211347013 1102 30-31 -0.5533258079098502 1102 32-33 -0.5598726644847929 1102 34-35 -0.5705874865821627 1102 36-37 -0.46419311809261643 1102 38-39 -0.6074186391711507 1102 40-41 -0.7013747259383436 1102 42-43 -0.6073101142612174 1102 44-45 -0.5499581088831107 1102 46-47 -0.6349334476987138 1102 48-49 -0.632756237836297 1102 50-51 -0.6009842644198855 1102 52-53 -0.680382448686295 1102 54-55 -0.6831575492838553 1102 56-57 -0.6041344158227702 1102 58-59 -0.7641047181202865 1102 60-61 -0.8149386296618104 1102 62-63 -0.764189310559793 1102 64-65 -0.8525788793526203 1102 66-67 -0.8237893398322313 1102 68-69 -0.8972405600226452 1102 70-71 -0.7513458691952195 1102 72-73 -0.7962397541530706 1102 74-75 -0.8291695687330076 1102 76-77 -0.596944916673344 1102 78-79 -0.6630546592633877 1102 80-81 -0.5059311125089678 1102 82-83 -0.5134560555645997 1102 84-85 -0.6786749729810992 1102 86-87 -0.8253657259308831 1102 88-89 -0.8475297806243454 1102 90-91 -0.865393671087272 1102 92-93 -0.980646057151823 1102 94-95 -0.9721688070058221 1102 96-97 -1.0600960012615772 1102 98-99 -1.1647097945655531 1102 100 -1.3452234141598858 1103 1 -0.3248088245194687 1103 2 -0.3014439718877746 1103 3 -0.39712521579100013 1103 4 -0.16489691834136977 1103 5 -0.19315130330888763 1103 6 -0.32068362292510955 1103 7 -0.25191693725984976 1103 8 -0.273832557512975 1103 9 -0.5355279911534083 1103 10-11 -0.46539652945140375 1103 12-13 -0.6243480088574955 1103 14-15 -0.8074805131343226 1103 16-17 -0.8691841466973287 1103 18-19 -0.7722263507302998 1103 20-21 -0.6403202857814279 1103 22-23 -0.716801831081689 1103 24-25 -0.731116789161355 1103 26-27 -0.8507279213514138 1103 28-29 -0.8159559204935221 1103 30-31 -0.6543946234903402 1103 32-33 -0.7193097462667666 1103 34-35 -0.6299529167621571 1103 36-37 -0.5899540303996034 1103 38-39 -0.5834161035756793 1103 40-41 -0.8250947442576475 1103 42-43 -0.9067390378081299 1103 44-45 -0.7058741978973373 1103 46-47 -0.7730855974470501 1103 48-49 -0.7549249731029093 1103 50-51 -0.962416608817577 1103 52-53 -0.8769064530578348 1103 54-55 -0.8636470667529679 1103 56-57 -0.8823055291281037 1103 58-59 -0.981171602295376 1103 60-61 -1.051202067883871 1103 62-63 -1.0410105931384948 1103 64-65 -0.8885553105987896 1103 66-67 -1.0125590986836102 1103 68-69 -1.023343290360934 1103 70-71 -0.8904134002876418 1103 72-73 -0.9514999965100657 1103 74-75 -0.9789978585813444 1103 76-77 -0.9532257896178464 1103 78-79 -0.8482780560400194 1103 80-81 -0.7985244264948648 1103 82-83 -0.7039349045724954 1103 84-85 -0.8944945050776134 1103 86-87 -0.9017324305847474 1103 88-89 -0.8867068336855901 1103 90-91 -1.073148100201088 1103 92-93 -1.128515486001934 1103 94-95 -1.1487796578589986 1103 96-97 -1.1633247129080253 1103 98-99 -1.1651204602227914 1103 100 -1.2191241053399793 1104 1 -0.3446084895110957 1104 2 -0.2965662499447248 1104 3 -0.3026468238311999 1104 4 -0.14672204396951116 1104 5 -0.23756604367739698 1104 6 -0.36228965307586947 1104 7 -0.2829916441275202 1104 8 -0.25605031295685876 1104 9 -0.4644599844532422 1104 10-11 -0.41976101856363357 1104 12-13 -0.4107466688239967 1104 14-15 -0.6307203791309703 1104 16-17 -0.6205094398296538 1104 18-19 -0.6758323808810545 1104 20-21 -0.7046442388802561 1104 22-23 -0.7215214290716361 1104 24-25 -0.8872330370675527 1104 26-27 -0.8548565645674913 1104 28-29 -0.8889736759374074 1104 30-31 -0.9003081913295361 1104 32-33 -0.7603358769200312 1104 34-35 -0.7377127157571337 1104 36-37 -0.7160984190093203 1104 38-39 -0.8544422342289408 1104 40-41 -0.9458160172894807 1104 42-43 -0.8472583007897114 1104 44-45 -1.0188571124701937 1104 46-47 -0.8919207733264471 1104 48-49 -0.9013269831531616 1104 50-51 -0.907271550191119 1104 52-53 -0.8835020979489556 1104 54-55 -0.9123974855134307 1104 56-57 -0.8462284772018052 1104 58-59 -0.8376888552266948 1104 60-61 -0.9073230059073225 1104 62-63 -0.9036618494198976 1104 64-65 -0.9530390626925964 1104 66-67 -1.0096059998561344 1104 68-69 -1.0374742786356421 1104 70-71 -0.9904412059827834 1104 72-73 -1.0488574504464125 1104 74-75 -0.940060840155887 1104 76-77 -0.8479819035206901 1104 78-79 -0.8740639856882595 1104 80-81 -0.9131589323575113 1104 82-83 -0.990914804069984 1104 84-85 -0.8993407362333912 1104 86-87 -0.9183186952413678 1104 88-89 -0.9592405020272956 1104 90-91 -0.830183946097236 1104 92-93 -0.9278923704240434 1104 94-95 -0.9896141637216438 1104 96-97 -1.1335012623217615 1104 98-99 -1.157570376470698 1104 100 -1.0080527485560609 1105 1 -0.40837937857482487 1105 2 -0.37431290612096646 1105 3 -0.42037680022773927 1105 4 -0.29600386930389533 1105 5 -0.34531521755623373 1105 6 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68-69 -0.9112810449613704 1105 70-71 -1.2363154782093773 1105 72-73 -1.2566401412410642 1105 74-75 -1.1687958519576185 1105 76-77 -1.1387732489180173 1105 78-79 -1.1284026166874739 1105 80-81 -1.0578895381796976 1105 82-83 -0.9824285727560564 1105 84-85 -0.8092403428423616 1105 86-87 -0.9637467046827481 1105 88-89 -0.9287591487621505 1105 90-91 -1.1686676597085643 1105 92-93 -1.0989553129889558 1105 94-95 -1.110544454831004 1105 96-97 -1.1666859987497702 1105 98-99 -1.2635603056603095 1105 100 -1.4677245030800563 1106 1 -0.37417527259219696 1106 2 -0.3508898820751085 1106 3 -0.4042485312429207 1106 4 -0.2105700843265197 1106 5 -0.18982851942438828 1106 6 -0.3303219386792833 1106 7 -0.38202323124432525 1106 8 -0.3805384774892602 1106 9 -0.476299953409395 1106 10-11 -0.43465834103161427 1106 12-13 -0.50870747596408 1106 14-15 -0.5914878606986846 1106 16-17 -0.6370211976876377 1106 18-19 -0.6358130560847783 1106 20-21 -0.6008695396175412 1106 22-23 -0.6987229812641118 1106 24-25 -0.8269973366407015 1106 26-27 -0.7089129091542148 1106 28-29 -0.7663269549439988 1106 30-31 -0.7362040392146767 1106 32-33 -0.8244893111458751 1106 34-35 -0.8046542757105684 1106 36-37 -0.7708631842402056 1106 38-39 -0.9147307091998371 1106 40-41 -0.9428476044062748 1106 42-43 -0.7055858134012709 1106 44-45 -0.9075948695520779 1106 46-47 -0.8138518559807011 1106 48-49 -0.7750825128388428 1106 50-51 -0.8437153220188165 1106 52-53 -0.9285461026055302 1106 54-55 -0.7403606209422335 1106 56-57 -0.927120211776888 1106 58-59 -0.961888389568955 1106 60-61 -1.0469981320229635 1106 62-63 -0.9604411276504052 1106 64-65 -0.956673598975101 1106 66-67 -0.9263921853431398 1106 68-69 -1.0368847559348282 1106 70-71 -0.9816866076125876 1106 72-73 -0.9160815870393506 1106 74-75 -0.8984354618089725 1106 76-77 -0.8972386361555387 1106 78-79 -0.7956987547996306 1106 80-81 -0.7702897821828856 1106 82-83 -0.8304056849392119 1106 84-85 -0.7331664250188012 1106 86-87 -0.8154056180197884 1106 88-89 -0.9553070134747159 1106 90-91 -0.8955171242113238 1106 92-93 -0.842959114700335 1106 94-95 -0.7786746216184248 1106 96-97 -0.8826547741572277 1106 98-99 -1.090903088927039 1106 100 -0.9720510411443399 1107 1 -0.25070251588813264 1107 2 -0.21816749121703083 1107 3 -0.29713340257441345 1107 4 -0.18214081821311368 1107 5 -0.1753184098527285 1107 6 -0.18698662747462436 1107 7 -0.28169668679625914 1107 8 -0.3224909646248193 1107 9 -0.4362840341041334 1107 10-11 -0.3760316159259318 1107 12-13 -0.3982292134322165 1107 14-15 -0.5468061820789885 1107 16-17 -0.5141522637241493 1107 18-19 -0.5139172528748404 1107 20-21 -0.6195846578096607 1107 22-23 -0.5639487370623257 1107 24-25 -0.573624193002388 1107 26-27 -0.5916854241485581 1107 28-29 -0.5307314726790722 1107 30-31 -0.5793812712364499 1107 32-33 -0.6359478241659602 1107 34-35 -0.7113037165329317 1107 36-37 -0.7278917471706805 1107 38-39 -0.621272179923281 1107 40-41 -0.7156785463817954 1107 42-43 -0.72458180738397 1107 44-45 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2311 30-31 0.9002618775496103 2311 32-33 1.1365286190098445 2311 34-35 1.0550284238796834 2311 36-37 1.114855369343836 2311 38-39 1.0584272710935423 2311 40-41 1.071087927607202 2311 42-43 1.0774380047832395 2311 44-45 1.0775741962336198 2311 46-47 1.0993876800235824 2311 48-49 1.1411914889820949 2311 50-51 1.1044869970850257 2311 52-53 1.123534595851929 2311 54-55 1.0533063341484379 2311 56-57 1.1672104708257436 2311 58-59 1.2270361291189502 2311 60-61 1.2815180711120888 2311 62-63 1.1677394029282553 2311 64-65 1.2999489147651317 2311 66-67 1.3342336995803095 2311 68-69 1.2771225904939811 2311 70-71 1.2395316180623013 2311 72-73 1.258717627825348 2311 74-75 1.2050891911528154 2311 76-77 1.1265762680510036 2311 78-79 1.0591665716066672 2311 80-81 1.0306180244364782 2311 82-83 1.0159430544146737 2311 84-85 1.1037858761648565 2311 86-87 1.136331899624011 2311 88-89 1.2305544885800934 2311 90-91 1.234406160352357 2311 92-93 1.3422096959504088 2311 94-95 1.3708762558149878 2311 96-97 1.4213800150733569 2311 98-99 1.3996729547753937 2311 100 1.441816005357527 2312 1 0.15023512579929132 2312 2 0.07922532290827888 2312 3 0.32555864416494273 2312 4 0.30619567877091924 2312 5 0.29861060476261514 2312 6 0.39589420025318134 2312 7 0.46566232821085407 2312 8 0.3087287156697087 2312 9 0.44157501072205463 2312 10-11 0.28625480646048374 2312 12-13 0.43049971264915143 2312 14-15 0.7852389003801292 2312 16-17 0.8408660506817611 2312 18-19 0.8857715432102964 2312 20-21 0.8796033648508441 2312 22-23 0.4347079694487874 2312 24-25 0.43490456344707695 2312 26-27 0.8100728661176149 2312 28-29 0.8989228181121263 2312 30-31 0.9628306957724178 2312 32-33 0.7708452745756276 2312 34-35 0.8765675029627147 2312 36-37 0.9405083355741439 2312 38-39 0.9523323556713521 2312 40-41 0.9614477974419486 2312 42-43 0.890126710467932 2312 44-45 0.9066197611225917 2312 46-47 1.124438570514016 2312 48-49 1.0885231294232298 2312 50-51 1.1150169026876213 2312 52-53 1.1710515205779117 2312 54-55 1.1531744025534678 2312 56-57 1.1356029798583407 2312 58-59 1.1606908810228944 2312 60-61 1.226949235971091 2312 62-63 1.259373403072864 2312 64-65 1.3385963827882676 2312 66-67 1.315358297345533 2312 68-69 1.3689607165396538 2312 70-71 1.3038882247023196 2312 72-73 1.3275779950344457 2312 74-75 1.3258787545691106 2312 76-77 1.2829192865725858 2312 78-79 1.2234215080396282 2312 80-81 1.0769905047605306 2312 82-83 1.007651937647612 2312 84-85 0.8812994696205791 2312 86-87 1.101243543420587 2312 88-89 0.6207730714690065 2312 90-91 1.0595165363730104 2312 92-93 1.2980777164206003 2312 94-95 1.3769443438370566 2312 96-97 1.4869856466521831 2312 98-99 1.5306146347869465 2312 100 1.5914836940299786 2313 1 -0.552271753531258 2313 2 -0.16704640117345804 2313 3 0.15569243262752508 2313 4 0.037859934606416346 2313 5 0.19265928713545577 2313 6 0.33398306274013834 2313 7 0.11708623867021117 2313 8 0.14465164041428125 2313 9 0.4295415278340897 2313 10-11 0.46653866637650054 2313 12-13 0.5756774017494095 2313 14-15 0.541483905683144 2313 16-17 0.7093552104538929 2313 18-19 0.6871819858486319 2313 20-21 0.8569747907353715 2313 22-23 0.8650630699831865 2313 24-25 0.6811912855537443 2313 26-27 0.8387671279340836 2313 28-29 0.7636026315610209 2313 30-31 0.7874538754631502 2313 32-33 1.0207508275538189 2313 34-35 1.0014804789498655 2313 36-37 0.7109823623391378 2313 38-39 0.5720508974660774 2313 40-41 0.8054742158548223 2313 42-43 0.7375180067087186 2313 44-45 0.9702965107182067 2313 46-47 1.1619554711599989 2313 48-49 1.1879874465884939 2313 50-51 1.0726001574333353 2313 52-53 0.93529412133271 2313 54-55 0.9386793890927407 2313 56-57 1.0004961604793507 2313 58-59 1.0063261416227007 2313 60-61 0.9490999304505863 2313 62-63 1.0009719249972377 2313 64-65 1.0352267218064526 2313 66-67 1.0497210322799617 2313 68-69 1.1326046123536457 2313 70-71 1.0132693170165865 2313 72-73 1.2130867209461513 2313 74-75 0.9029678933034759 2313 76-77 0.9860555255908423 2313 78-79 0.8474125108460768 2313 80-81 0.9972807651850246 2313 82-83 0.9464147485450205 2313 84-85 0.9952321686185286 2313 86-87 1.0259894333030601 2313 88-89 1.103200959850465 2313 90-91 1.2282082404182049 2313 92-93 0.9006287385866685 2313 94-95 0.9627155149172957 2313 96-97 1.3003761163801926 2313 98-99 1.2519819864782669 2313 100 1.4324062306499883 2314 1 -0.15743601531653795 2314 2 -0.10811207415492419 2314 3 0.13235986994421722 2314 4 -0.1441947996779902 2314 5 0.1875185981743357 2314 6 0.2284606753570415 2314 7 0.13844457733009818 2314 8 0.15334411932339975 2314 9 0.24026081629746443 2314 10-11 0.3985016026437549 2314 12-13 0.4880193618297426 2314 14-15 0.4710419605624949 2314 16-17 0.7571887772488495 2314 18-19 0.8236414383244508 2314 20-21 0.463410625054685 2314 22-23 0.7045491370841361 2314 24-25 0.8400335878933447 2314 26-27 0.8513695223890281 2314 28-29 0.8532836277848332 2314 30-31 0.6631514270627008 2314 32-33 0.7912977170743432 2314 34-35 0.8388551916560658 2314 36-37 0.7134427758608695 2314 38-39 0.727238278751976 2314 40-41 0.8639824187443068 2314 42-43 0.7273397598921818 2314 44-45 0.74435552437685 2314 46-47 0.847209536338859 2314 48-49 0.7046323537251666 2314 50-51 0.7987179399558997 2314 52-53 0.9011452589481408 2314 54-55 0.9315746758878802 2314 56-57 0.8115951074087704 2314 58-59 0.7488315701721078 2314 60-61 0.8718728652218601 2314 62-63 0.5867566053814741 2314 64-65 0.8547688196971421 2314 66-67 0.9267286889145794 2314 68-69 0.9418098001882598 2314 70-71 1.105116066460134 2314 72-73 1.0207914078582476 2314 74-75 1.0177698135819924 2314 76-77 0.811289100545622 2314 78-79 0.8586164021753646 2314 80-81 0.8186214242267837 2314 82-83 0.7680942669340816 2314 84-85 0.7300628296095866 2314 86-87 0.8378986960629788 2314 88-89 0.8623524288453943 2314 90-91 0.9837104549037008 2314 92-93 1.1458495751499314 2314 94-95 1.156198535684048 2314 96-97 1.1819555409312876 2314 98-99 1.214089804949385 2314 100 1.1663483774996166 2315 1 -0.03675425018457901 2315 2 0.14237061114967986 2315 3 0.11498131808100709 2315 4 -0.04319324060210761 2315 5 0.27435963281087794 2315 6 0.3555787774713366 2315 7 0.4059298231003794 2315 8 0.3468276184923269 2315 9 0.5771032145846391 2315 10-11 0.5411784883515836 2315 12-13 0.6058167707430542 2315 14-15 0.6156186467259133 2315 16-17 0.7315379444156846 2315 18-19 0.7667089300028991 2315 20-21 0.8397698320758522 2315 22-23 0.8755563821129684 2315 24-25 0.820109476462207 2315 26-27 0.8601279213014195 2315 28-29 0.8934745501600077 2315 30-31 0.8843710630300521 2315 32-33 1.001549510933252 2315 34-35 0.9300566167744364 2315 36-37 0.8853283999924813 2315 38-39 0.9929753244000423 2315 40-41 0.924329743384007 2315 42-43 0.9687128357105905 2315 44-45 0.9695753589669565 2315 46-47 0.9504414635947711 2315 48-49 0.950902722198613 2315 50-51 0.9069759543188027 2315 52-53 0.8914049375290958 2315 54-55 0.7945123160500032 2315 56-57 0.8961034530447378 2315 58-59 0.9631819806121484 2315 60-61 1.0623443422586405 2315 62-63 1.0705691788422769 2315 64-65 1.0915623804823866 2315 66-67 1.139881673024199 2315 68-69 1.078902630564599 2315 70-71 0.6846708806077118 2315 72-73 0.9637370173828117 2315 74-75 1.0766038621892662 2315 76-77 1.046806190285679 2315 78-79 0.8715564592906944 2315 80-81 0.8948279588030417 2315 82-83 0.9520845946071113 2315 84-85 0.9022304002669088 2315 86-87 0.9917131748728849 2315 88-89 1.1616286501073994 2315 90-91 1.2233227285870711 2315 92-93 1.2629597041399947 2315 94-95 1.308252823650573 2315 96-97 1.342197234370964 2315 98-99 1.198497212224435 2315 100 1.1103426813898203 2316 1 0.39888031915316446 2316 2 0.4295757476485349 2316 3 0.38145329650574666 2316 4 0.24223198731809248 2316 5 0.42030254838036285 2316 6 0.4014075791744318 2316 7 0.4270767072563544 2316 8 0.4241927676688917 2316 9 0.44466781338068984 2316 10-11 0.4120185241559824 2316 12-13 0.5689946067476086 2316 14-15 0.7473982730952642 2316 16-17 0.7147584302064445 2316 18-19 0.7722174386135308 2316 20-21 0.7771574458369557 2316 22-23 0.7480752014939398 2316 24-25 0.8664098028843128 2316 26-27 0.9430741213530851 2316 28-29 0.925992389044545 2316 30-31 0.7192576329785183 2316 32-33 0.7139538944397756 2316 34-35 0.5519440832801692 2316 36-37 0.663532868595972 2316 38-39 0.6117308102957324 2316 40-41 0.6789779658633464 2316 42-43 0.7699438652752733 2316 44-45 0.6776030560015229 2316 46-47 0.6947288054942504 2316 48-49 0.46852379903697283 2316 50-51 0.5172750202060001 2316 52-53 0.6820752787056819 2316 54-55 0.804470143848782 2316 56-57 0.8389111136525855 2316 58-59 0.7938134311752236 2316 60-61 0.8477390879554889 2316 62-63 0.9502543960674643 2316 64-65 0.905464427078762 2316 66-67 0.86635789906083 2316 68-69 0.8049656732295816 2316 70-71 0.7326175184456147 2316 72-73 0.746281177712433 2316 74-75 0.7514383174855084 2316 76-77 0.6994804310160134 2316 78-79 0.7422135113033193 2316 80-81 0.6720232577748604 2316 82-83 0.5731598048349547 2316 84-85 0.6978958461974116 2316 86-87 0.7231927367682616 2316 88-89 0.6973657554937631 2316 90-91 0.7833289299557151 2316 92-93 0.752635908757668 2316 94-95 0.7146681467476697 2316 96-97 0.9203064392426192 2316 98-99 0.9481875055991011 2316 100 0.7253999590251681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 4.0 12 15.0 13 88.0 14 388.0 15 1080.0 16 2690.0 17 5373.0 18 8510.0 19 11713.0 20 15266.0 21 19746.0 22 23168.0 23 24041.0 24 24025.0 25 26051.0 26 29693.0 27 35711.0 28 43197.0 29 52414.0 30 63945.0 31 78555.0 32 99196.0 33 127365.0 34 177376.0 35 243361.0 36 366002.0 37 582838.0 38 553793.0 39 86875.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2894426052774 19.63391238094674 13.181805055862386 23.894839957913476 2 15.983131131144464 21.189953128463237 39.986597560104684 22.840318180287607 3 18.35112986416616 26.86852472883371 31.08955323843577 23.69079216856436 4 12.6609997183673 17.50363883511386 38.29756918782829 31.53779225869055 5 12.561876310656547 37.822656952220036 34.56255201458573 15.052914722537686 6 29.155423956851678 37.18157167532781 19.272915516493665 14.39008885132685 7 26.00855211938036 33.796375784092284 22.820452856774942 17.374619239752406 8 25.584508764717484 35.35963038435395 21.01870761074168 18.037153240186896 9 27.332716853183364 15.95414364579552 20.722465890996986 35.99067361002413 10-11 24.462177193456355 26.066542829337607 29.323304236494764 20.147975740711274 12-13 24.899772783155647 24.410630292236643 28.541310782726043 22.148286141881666 14-15 22.651722928319494 26.018596909815088 26.769847977348316 24.559832184517106 16-17 21.756553165847144 28.250634645844396 27.59022553817468 22.402586650133774 18-19 21.283607395097786 27.729156005214445 29.563998870672375 21.423237729015394 20-21 22.298115110751443 27.27028340128911 29.071934691412704 21.359666796546744 22-23 22.089437419449375 26.924269554074698 29.055819941069227 21.930473085406696 24-25 21.616868745811733 26.675853259717247 29.450462148042046 22.256815846428974 26-27 21.585976355905455 26.927988342615507 29.240057236040943 22.245978065438095 28-29 21.430466526621476 27.952170262586584 29.049817925217052 21.567545285574887 30-31 22.647733317298574 26.957376836866377 29.276483947510833 21.118405898324212 32-33 22.25834655613484 27.09753107551106 28.91531084763169 21.728811520722406 34-35 22.27712703992361 27.26212341740235 29.12568159379832 21.33506794887572 36-37 21.93014129236851 27.09626554711118 29.127918220876552 21.845674939643757 38-39 21.83406040808713 27.24294881830459 29.13878374772338 21.784207025884896 40-41 21.885100649882865 26.56007225936702 29.68019827662446 21.874628814125654 42-43 21.627157654603195 26.931229804149392 28.978359391550423 22.46325314969699 44-45 20.93303861204183 27.282942801717407 29.541572207086485 22.24244637915428 46-47 20.833681059733138 26.573766508831266 29.28155438974433 23.31099804169127 48-49 21.069070560381416 26.74500747130818 29.35733797918691 22.82858398912349 50-51 20.986491342402264 26.95923274170276 29.197211302079456 22.85706461381552 52-53 20.739707781507658 27.261210779135205 28.808607971677837 23.190473467679304 54-55 20.43913217892278 26.863230030463885 29.241925886013554 23.45571190459978 56-57 20.94589006461487 26.69846930307969 28.607034533977526 23.748606098327908 58-59 20.438934708781247 26.833279859317077 28.925113063313606 23.80267236858807 60-61 21.184517120250085 26.92332456225719 28.46156237152828 23.43059594596445 62-63 21.072659129464007 26.7752565577715 28.291596383519245 23.860487929245245 64-65 20.57265932823033 26.421183609690523 28.25153648973441 24.75462057234474 66-67 20.686290769812846 26.408021317916987 27.90750191951583 24.998185992754337 68-69 20.345989130382467 26.58665204450479 28.031139725197647 25.0362190999151 70-71 20.546015256662525 26.30432247423749 27.99105184340541 25.158610425694572 72-73 20.491625166073323 26.547744160641628 27.544165602082206 25.416465071202836 74-75 20.61046861322244 26.488260147796105 27.406128374964606 25.495142864016852 76-77 20.317676467545464 26.251726009691378 27.550122062961208 25.88047545980195 78-79 20.22271154451085 26.212164387617264 27.631756194141705 25.93336787373018 80-81 20.16611737636528 26.31009576023622 27.464780263219 26.059006600179508 82-83 20.46235324751948 25.929002863196715 27.633929075410098 25.974714813873707 84-85 20.57643959034968 26.121631182174294 27.107310154664727 26.1946190728113 86-87 20.058627488873142 26.15385384295859 27.459959231756287 26.327559436411978 88-89 20.137395131521924 25.53174985333177 27.564814953661354 26.76604006148495 90-91 20.603714009944373 25.057138970452826 27.60752944399048 26.731617575612315 92-93 20.42234415124397 25.13165521361308 27.545124389563224 26.900876245579724 94-95 19.939280768715534 24.956129541267835 27.800271179014413 27.30431851100221 96-97 19.900025477461885 24.7794682370799 27.96957210260479 27.350934182853425 98-99 19.831519954934798 24.835049001374237 27.79372973389102 27.53970130979994 100 19.659586468878576 25.016127247405485 27.925619617542747 27.398666666173195 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1358.0 1 957.0 2 1126.0 3 1645.5 4 1192.5 5 846.5 6 1150.0 7 1780.0 8 2576.5 9 2985.0 10 2575.0 11 2112.5 12 2180.5 13 2400.5 14 2627.5 15 2891.5 16 3185.0 17 3564.5 18 3863.5 19 4106.5 20 4565.0 21 5155.0 22 5844.0 23 6858.0 24 8130.5 25 9725.5 26 11893.0 27 14609.5 28 17883.0 29 21841.5 30 26930.0 31 31925.0 32 38036.5 33 44691.5 34 50241.5 35 55846.0 36 61617.0 37 68527.0 38 73479.5 39 75635.5 40 77033.5 41 78802.5 42 81644.0 43 85930.5 44 91107.0 45 97987.0 46 104952.5 47 110405.5 48 116270.5 49 120591.5 50 122671.0 51 123054.5 52 120505.5 53 114499.5 54 105091.0 55 92490.0 56 80468.0 57 68636.5 58 56266.5 59 44937.0 60 34003.5 61 25159.0 62 19178.5 63 14436.0 64 10279.0 65 7166.5 66 5129.0 67 3903.5 68 3005.0 69 2245.0 70 1718.0 71 1354.0 72 1046.5 73 794.0 74 600.5 75 433.0 76 297.5 77 189.5 78 129.0 79 74.5 80 42.5 81 31.5 82 19.5 83 11.5 84 8.5 85 7.0 86 2.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05147099363439609 2 0.0 3 0.0 4 0.014505125452683875 5 0.0046623617526483875 6 3.700287105276498E-5 7 1.8501435526382492E-4 8 3.7002871052764985E-4 9 0.001998155036849309 10-11 8.510660342135947E-4 12-13 3.1452440394850236E-4 14-15 3.700287105276498E-5 16-17 5.550430657914748E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4801148421105992E-4 26-27 0.0 28-29 0.0011840918736884794 30-31 0.001128587567109332 32-33 0.015004664211896201 34-35 0.0238853532645598 36-37 0.03054587005405749 38-39 0.021091636500076043 40-41 2.2201722631658992E-4 42-43 0.0018686449881646318 44-45 0.01032380102372143 46-47 0.015411695793476617 48-49 0.005735445013178572 50-51 0.011100861315829495 52-53 0.01626276182769021 54-55 0.010249795281615902 56-57 0.0035707770565918213 58-59 0.009102706278980187 60-61 0.001850143552638249 62-63 0.0011655904381620969 64-65 0.020536593434284565 66-67 0.0477707065291196 68-69 0.058094507552841027 70-71 0.05098995631071015 72-73 0.04166523280541337 74-75 0.02952829110010646 76-77 0.030101835601424317 78-79 0.01269198477109839 80-81 0.0024606909250088713 82-83 0.02932477530931625 84-85 0.050323904631760376 86-87 0.050730936213340794 88-89 0.061776293222591136 90-91 0.03572627200144459 92-93 0.02734512170799332 94-95 0.008103628760555532 96-97 0.003866800025013941 98-99 0.02331180876324194 100 0.02012956185270415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2702493.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.94654162860643 #Duplication Level Percentage of deduplicated Percentage of total 1 84.16792836940063 60.555913622226456 2 10.610505023322226 15.267782827219802 3 2.523018072082144 5.445672742583529 4 0.9729397784786493 2.7999860909776504 5 0.5024494140771145 1.8074748843084014 6 0.2995431486051115 1.2930656166408898 7 0.19254523866770865 0.9697074820437382 8 0.13614405427977994 0.7836075094981952 9 0.09710332524967957 0.6287623589116963 >10 0.45760985508551844 5.610713326394853 >50 0.02568734986637234 1.2818111851116805 >100 0.013219664991520401 1.7188806416592213 >500 6.5335294679488E-4 0.2906959375579621 >1k 4.900147100739433E-4 0.6148448235726603 >5k 1.6333823669131443E-4 0.9310809512932318 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9976 0.36914064162238347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8595 0.3180396766985151 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5198 0.19234092373227238 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2771 0.10253495568721177 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.700287105276498E-5 2 0.0 0.0 0.0 0.0 3.700287105276498E-5 3 0.0 0.0 0.0 0.0 3.700287105276498E-5 4 0.0 0.0 0.0 0.0 3.700287105276498E-5 5 0.0 0.0 0.0 3.700287105276498E-5 3.700287105276498E-5 6 0.0 0.0 0.0 3.700287105276498E-5 3.700287105276498E-5 7 0.0 0.0 0.0 3.700287105276498E-5 3.700287105276498E-5 8 0.0 0.0 0.0 3.700287105276498E-5 3.700287105276498E-5 9 0.0 0.0 0.0 7.400574210552996E-5 3.700287105276498E-5 10-11 0.0 0.0 0.0 1.665129197374424E-4 3.700287105276498E-5 12-13 0.0 0.0 0.0 1.8501435526382492E-4 3.700287105276498E-5 14-15 0.0 0.0 0.0 1.8501435526382492E-4 7.400574210552996E-5 16-17 0.0 0.0 0.0 2.220172263165899E-4 7.400574210552996E-5 18-19 0.0 0.0 0.0 4.625358881595623E-4 7.400574210552996E-5 20-21 0.0 0.0 0.0 0.001054581825003802 7.400574210552996E-5 22-23 0.0 0.0 0.0 0.0037372899763292635 9.250717763191246E-5 24-25 0.0 0.0 0.0 0.010675328298722697 1.1100861315829495E-4 26-27 0.0 0.0 0.0 0.0220907140185007 1.1100861315829495E-4 28-29 0.0 0.0 0.0 0.05770597740678699 1.1100861315829495E-4 30-31 0.0 0.0 0.0 0.16185055798479403 1.2951004868467744E-4 32-33 0.0 0.0 0.0 0.38804910873034637 1.4801148421105992E-4 34-35 0.0 0.0 0.0 0.7186512601512751 1.8501435526382492E-4 36-37 0.0 0.0 0.0 1.1974869130095804 1.8501435526382492E-4 38-39 0.0 0.0 0.0 1.8751574934699184 1.8501435526382492E-4 40-41 0.0 0.0 0.0 2.611015088660729 1.8501435526382492E-4 42-43 0.0 0.0 0.0 3.288204631797381 1.8501435526382492E-4 44-45 0.0 0.0 0.0 4.06350728753044 1.8501435526382492E-4 46-47 0.0 0.0 0.0 4.944249624328352 1.8501435526382492E-4 48-49 0.0 0.0 0.0 5.814409140005173 1.8501435526382492E-4 50-51 0.0 0.0 0.0 6.682477993467513 1.8501435526382492E-4 52-53 0.0 0.0 0.0 7.512581901229716 2.035157907902074E-4 54-55 0.0 0.0 0.0 8.42379610233958 2.220172263165899E-4 56-57 0.0 0.0 0.0 9.402411033072056 2.220172263165899E-4 58-59 0.0 0.0 0.0 10.44900023792846 2.220172263165899E-4 60-61 0.0 3.700287105276498E-5 0.0 11.460177695187369 2.220172263165899E-4 62-63 0.0 3.700287105276498E-5 0.0 12.474518897921289 2.220172263165899E-4 64-65 0.0 3.700287105276498E-5 0.0 13.459960858363 2.220172263165899E-4 66-67 0.0 3.700287105276498E-5 0.0 14.52268331499841 2.220172263165899E-4 68-69 0.0 3.700287105276498E-5 0.0 15.671640962622291 2.220172263165899E-4 70-71 0.0 3.700287105276498E-5 0.0 16.826241548081715 2.220172263165899E-4 72-73 0.0 3.700287105276498E-5 0.0 17.87938026111446 2.5902009736935487E-4 74-75 0.0 3.700287105276498E-5 0.0 18.902639155772096 2.5902009736935487E-4 76-77 0.0 3.700287105276498E-5 0.0 19.98957999151154 2.5902009736935487E-4 78-79 0.0 3.700287105276498E-5 0.0 21.140517292736742 2.5902009736935487E-4 80-81 0.0 3.700287105276498E-5 0.0 22.247199160182838 2.775215328957374E-4 82-83 0.0 3.700287105276498E-5 0.0 23.341078034244674 2.9602296842211985E-4 84-85 0.0 3.700287105276498E-5 0.0 24.41070152633143 2.9602296842211985E-4 86-87 0.0 3.700287105276498E-5 0.0 25.471148306397094 2.9602296842211985E-4 88 0.0 3.700287105276498E-5 0.0 26.284434409265813 2.9602296842211985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5180 0.0 49.656948 1 TACCTGG 2285 0.0 46.2749 2 GTATCAA 9020 0.0 39.41284 1 ACCTGGG 2635 0.0 38.52319 3 ATCAACG 10860 0.0 32.02219 3 CAACGCA 10985 0.0 31.996498 5 TATCAAC 11315 0.0 31.39904 2 TCAACGC 11235 0.0 31.247316 4 AACGCAG 11705 0.0 30.387379 6 CCTGGGG 2635 0.0 28.3584 4 TAACGCA 290 0.0 27.549652 4 ACGCAGA 13115 0.0 26.905457 7 GTACATG 8830 0.0 26.574327 1 CGCAGAG 13380 0.0 26.337461 8 TATTCGC 145 8.680199E-7 25.923326 9 TACATGG 9070 0.0 25.647615 2 ATAACGC 315 0.0 25.362234 3 TGGGGGG 5790 0.0 24.994297 6 ATGGGGG 5240 0.0 24.57085 5 GTACCTG 4400 0.0 24.26027 1 >>END_MODULE