FastQCFastQC Report
Mon 6 Jun 2016
ERR1211387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4588370
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184140.4013189869169226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145010.31603815734127805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86640.188825225515815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46850.10210597663222452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.054.499631
GTATCAA143350.042.352821
TACCTGG40150.040.7875062
TAACGCA5300.035.4786764
CAACGCA174050.033.7883155
ATCAACG174900.033.6509863
TATCAAC178100.033.5562362
ATAACGC5350.033.3897443
TCAACGC181400.032.497024
AACGCAG186850.031.8514046
ACCTGGG52350.030.6211133
ACGCAGA204550.028.7275457
CGCAGAG208650.028.0933078
GTACATG143500.027.8993511
TACATGG143450.026.702832
GTGGTAT31650.026.4894771
ACATGGG140300.025.967333
GCAGAGT226600.025.2041029
CATGGGG105700.024.7721374
GTACCTG69750.023.9048561
Produced by FastQC (version 0.11.5)