FastQCFastQC Report
Mon 6 Jun 2016
ERR1211387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4588370
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163990.35740360956069367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136580.2976656198170592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87630.19098285447773392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74800.053.429261
TACCTGG41250.045.1129342
GTATCAA134450.040.1460531
ACCTGGG54850.033.241833
ATCAACG165400.032.133423
CAACGCA167950.031.6970025
TATCAAC170100.031.4665582
TCAACGC171900.031.0007174
AACGCAG180800.029.9639576
GTGGTAT30700.028.1800171
CCTGGGG51300.026.5653294
ACGCAGA203300.026.1854977
GTACCTG72900.025.9274831
CGCAGAG205800.025.776088
CCGCGTA1101.02113074E-425.6284399
GTACATG139400.025.3302211
TACATGG138000.025.062742
ACATGGG137200.024.2498443
ATAACGC4950.023.7336543
TGGGGGG79900.023.4047836
Produced by FastQC (version 0.11.5)