##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4588370 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00418122339742 33.0 31.0 34.0 30.0 34.0 2 32.136402905607 33.0 31.0 34.0 30.0 34.0 3 32.21348997574302 34.0 31.0 34.0 30.0 34.0 4 35.87600128150084 37.0 35.0 37.0 35.0 37.0 5 35.72523270791152 37.0 35.0 37.0 35.0 37.0 6 35.76233651601767 37.0 35.0 37.0 33.0 37.0 7 35.69344756416767 37.0 35.0 37.0 33.0 37.0 8 35.67791241769953 37.0 35.0 37.0 33.0 37.0 9 37.25389975089193 39.0 37.0 39.0 34.0 39.0 10-11 37.22901618657606 39.0 37.0 39.0 33.0 39.0 12-13 37.14445663710642 39.0 37.0 39.0 33.0 39.0 14-15 38.32841630034195 40.0 38.0 41.0 33.0 41.0 16-17 38.31308275051924 40.0 38.0 41.0 33.0 41.0 18-19 38.31941953242655 40.0 38.0 41.0 33.5 41.0 20-21 38.27582583793373 40.0 38.0 41.0 34.0 41.0 22-23 38.16642762026602 40.0 37.5 41.0 33.0 41.0 24-25 38.03890564623167 40.0 37.0 41.0 33.0 41.0 26-27 37.90556711860639 40.0 37.0 41.0 33.0 41.0 28-29 37.81537321532483 40.0 37.0 41.0 33.0 41.0 30-31 37.85693852065113 40.0 37.0 41.0 33.0 41.0 32-33 37.681489287045295 40.0 37.0 41.0 33.0 41.0 34-35 37.55324766747233 40.0 36.0 41.0 32.0 41.0 36-37 37.39105858943372 40.0 36.0 41.0 32.0 41.0 38-39 37.26234272737378 40.0 36.0 41.0 31.0 41.0 40-41 37.18785440145411 39.0 36.0 41.0 31.0 41.0 42-43 37.04102785956668 39.0 36.0 41.0 31.0 41.0 44-45 36.874173726181624 39.0 35.5 41.0 30.5 41.0 46-47 36.64688168129423 39.0 35.0 41.0 30.0 41.0 48-49 36.414282196073984 39.0 35.0 41.0 30.0 41.0 50-51 36.223199306071656 39.0 35.0 40.0 29.0 41.0 52-53 36.01050046094801 39.0 35.0 40.0 28.5 41.0 54-55 35.79910273147109 38.0 35.0 40.0 28.0 41.0 56-57 35.56350534067654 38.0 34.0 40.0 27.5 41.0 58-59 35.289410618585684 38.0 34.0 40.0 27.0 41.0 60-61 35.02471008222964 37.5 34.0 40.0 26.5 41.0 62-63 34.72494175491515 37.0 34.0 40.0 26.0 41.0 64-65 34.36721700734684 36.0 33.0 39.0 26.0 41.0 66-67 34.0073598031545 36.0 33.0 39.0 25.5 40.5 68-69 33.62428237042785 35.0 32.5 39.0 25.0 40.0 70-71 33.2403630265214 35.0 32.0 38.0 24.5 40.0 72-73 32.879912256422216 35.0 32.0 37.0 24.0 39.5 74-75 32.506172453398484 35.0 31.0 37.0 23.0 39.0 76-77 31.483494683297117 34.0 30.5 35.5 22.5 38.0 78-79 31.95821130379634 34.0 31.0 36.0 23.0 38.0 80-81 31.836538029845023 34.0 31.0 35.0 23.0 37.0 82-83 31.558741666430564 34.0 31.0 35.0 22.5 37.0 84-85 31.28480408075199 34.0 31.0 35.0 21.5 36.0 86-87 31.022267711627443 34.0 30.5 35.0 20.0 36.0 88-89 30.779565401220914 34.0 30.0 35.0 20.0 35.5 90-91 30.55277113659099 34.0 30.0 35.0 19.5 35.0 92-93 30.35766426857468 34.0 30.0 35.0 19.0 35.0 94-95 30.179123850081837 34.0 29.0 35.0 18.0 35.0 96-97 29.997351129050188 34.0 29.0 35.0 18.0 35.0 98-99 29.779820720648075 33.5 29.0 35.0 18.0 35.0 100 27.936893929652577 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31853503108613523 1101 2 -0.31365785497413157 1101 3 -0.38819440751444745 1101 4 -0.24462862605456337 1101 5 -0.18637849657827843 1101 6 -0.20370830845738652 1101 7 -0.32151037993092046 1101 8 -0.4092519229335849 1101 9 -0.47568451832327696 1101 10-11 -0.4358334972231006 1101 12-13 -0.5013682268007997 1101 14-15 -0.7898701856904538 1101 16-17 -0.6885527952220869 1101 18-19 -0.6711646393940285 1101 20-21 -0.7365143019791134 1101 22-23 -0.8642553696641357 1101 24-25 -0.8962466052447979 1101 26-27 -0.8086857701695038 1101 28-29 -0.8153535517472292 1101 30-31 -0.7884822656396793 1101 32-33 -0.6009392011802106 1101 34-35 -0.6652133051514681 1101 36-37 -0.7733966624747524 1101 38-39 -0.8425393450018035 1101 40-41 -0.7072659620336381 1101 42-43 -0.7970257191651982 1101 44-45 -0.6886148990365868 1101 46-47 -0.7579304179671809 1101 48-49 -0.7419599937977779 1101 50-51 -0.6895372996644014 1101 52-53 -0.8608487521944497 1101 54-55 -0.7565573263656304 1101 56-57 -0.8294476856581525 1101 58-59 -0.8531542182776732 1101 60-61 -0.8183728540149886 1101 62-63 -1.0274746927730192 1101 64-65 -0.8633069737187355 1101 66-67 -0.8491994951927353 1101 68-69 -0.8750600342943571 1101 70-71 -0.9400799384824481 1101 72-73 -0.9904502915065976 1101 74-75 -0.9869293473873313 1101 76-77 -0.8366807711630031 1101 78-79 -0.8052129263191077 1101 80-81 -0.6466663015951184 1101 82-83 -0.793799213777092 1101 84-85 -0.850150553792556 1101 86-87 -1.023923955421882 1101 88-89 -1.0421199336268678 1101 90-91 -1.201936069629408 1101 92-93 -1.3192030595673216 1101 94-95 -1.3590105221155646 1101 96-97 -1.499584807120609 1101 98-99 -1.6423301069173242 1101 100 -1.6037544995086606 1102 1 -0.3010834202127377 1102 2 -0.3046134930310167 1102 3 -0.3836653362836415 1102 4 -0.25913988958338763 1102 5 -0.2715094429663978 1102 6 -0.3191657084070485 1102 7 -0.31619015591883937 1102 8 -0.3472198313151651 1102 9 -0.5491865319150264 1102 10-11 -0.4823472777415958 1102 12-13 -0.5208685728868758 1102 14-15 -0.6752727467274511 1102 16-17 -0.7225516940390975 1102 18-19 -0.7490520041058275 1102 20-21 -0.7496252382992736 1102 22-23 -0.7402837172604109 1102 24-25 -0.773164677230703 1102 26-27 -0.8050420815211368 1102 28-29 -0.9133483604560055 1102 30-31 -0.8159152822518152 1102 32-33 -0.7772845950890925 1102 34-35 -0.8470081390301445 1102 36-37 -0.8234671696481684 1102 38-39 -0.9116188818756967 1102 40-41 -0.8294309192448139 1102 42-43 -0.934818161903685 1102 44-45 -0.9087478904788142 1102 46-47 -0.9655144539601324 1102 48-49 -1.075707067797282 1102 50-51 -1.100856737117212 1102 52-53 -1.070832486148639 1102 54-55 -1.1778699679463003 1102 56-57 -1.1564511779813316 1102 58-59 -1.1680568107770384 1102 60-61 -1.2902293856032188 1102 62-63 -1.1271578037722563 1102 64-65 -1.1228987494549543 1102 66-67 -1.172510532192483 1102 68-69 -1.241250538950787 1102 70-71 -1.1588959150744387 1102 72-73 -1.1694689172302333 1102 74-75 -1.2565517045481691 1102 76-77 -1.042159282363606 1102 78-79 -0.8990994415468947 1102 80-81 -0.9073748971148774 1102 82-83 -1.013867864670626 1102 84-85 -1.1417138246206449 1102 86-87 -1.2610369682585336 1102 88-89 -1.1793145598539034 1102 90-91 -1.2385441428738098 1102 92-93 -1.3186619382484182 1102 94-95 -1.3063399646023477 1102 96-97 -1.4213518282633224 1102 98-99 -1.5867229460816823 1102 100 -1.6100525110868134 1103 1 -0.3092175139242066 1103 2 -0.22123833890031364 1103 3 -0.3296061500937526 1103 4 -0.1604681879804275 1103 5 -0.08356030609833454 1103 6 -0.24316601666845372 1103 7 -0.2557841756475696 1103 8 -0.3697531235469782 1103 9 -0.4911538562060258 1103 10-11 -0.452452394880936 1103 12-13 -0.5041002313332399 1103 14-15 -0.738274442165185 1103 16-17 -0.7768446965051936 1103 18-19 -0.7659132864732783 1103 20-21 -0.7036594553153037 1103 22-23 -0.8298652524022074 1103 24-25 -0.8325130126191098 1103 26-27 -0.840072445269584 1103 28-29 -0.8118580706902847 1103 30-31 -0.7282277051864625 1103 32-33 -0.7624982202432236 1103 34-35 -0.7758970107934076 1103 36-37 -0.7054204680452045 1103 38-39 -0.721025786931186 1103 40-41 -0.8337408760882141 1103 42-43 -0.7724006218297035 1103 44-45 -0.8917397215515663 1103 46-47 -0.8020192320119222 1103 48-49 -0.7911634488083763 1103 50-51 -0.8512514658471773 1103 52-53 -1.062160938676385 1103 54-55 -1.0307048939635592 1103 56-57 -1.0384867821736918 1103 58-59 -1.073413160961998 1103 60-61 -1.0628533066882895 1103 62-63 -1.0562737100607933 1103 64-65 -1.080319372142995 1103 66-67 -1.0724448725130813 1103 68-69 -1.1313872528842026 1103 70-71 -1.1831053786995938 1103 72-73 -1.2222592994743735 1103 74-75 -1.0601369388268367 1103 76-77 -0.9623961812538653 1103 78-79 -0.9053844292164399 1103 80-81 -0.828192714624123 1103 82-83 -0.8037824762612544 1103 84-85 -0.8223042993432088 1103 86-87 -0.803268780835598 1103 88-89 -0.8230016745271449 1103 90-91 -1.1205637174730683 1103 92-93 -1.0701096879401568 1103 94-95 -1.1237824738501914 1103 96-97 -1.2516383572398944 1103 98-99 -1.3383144997191678 1103 100 -1.3136850634912527 1104 1 -0.4653069842598434 1104 2 -0.3487423683013837 1104 3 -0.39189845332281337 1104 4 -0.2505697742264985 1104 5 -0.2897588421492898 1104 6 -0.3121666096327118 1104 7 -0.37935964642592523 1104 8 -0.3825477572944962 1104 9 -0.5831953038448461 1104 10-11 -0.5249134277055489 1104 12-13 -0.5806606000438066 1104 14-15 -0.7584650382439122 1104 16-17 -0.7462149660890844 1104 18-19 -0.8014912014141728 1104 20-21 -0.7703196026347712 1104 22-23 -0.8235405966163327 1104 24-25 -0.8346418461804745 1104 26-27 -0.9013268387910216 1104 28-29 -0.9010220266420177 1104 30-31 -0.9518211041696247 1104 32-33 -0.8064652607944538 1104 34-35 -0.8562661599385848 1104 36-37 -0.8654331333588132 1104 38-39 -0.969412441116269 1104 40-41 -0.978292761350076 1104 42-43 -0.9293382592360544 1104 44-45 -1.0145337893012112 1104 46-47 -1.0083705282211284 1104 48-49 -1.0034026638078544 1104 50-51 -0.98742880512971 1104 52-53 -0.984623912488459 1104 54-55 -1.0056169939015902 1104 56-57 -1.078422969617968 1104 58-59 -1.1314415740551027 1104 60-61 -1.1342567828634884 1104 62-63 -1.299895847511742 1104 64-65 -1.1221836268882655 1104 66-67 -1.2603221760281045 1104 68-69 -1.1108719340481272 1104 70-71 -1.1655475913541764 1104 72-73 -1.0868837213638542 1104 74-75 -1.0145759475186082 1104 76-77 -0.9148745851754327 1104 78-79 -0.8867474528422576 1104 80-81 -1.072226074716415 1104 82-83 -1.0025865020174187 1104 84-85 -0.9896476446158395 1104 86-87 -0.9530941660475243 1104 88-89 -0.9440208203682019 1104 90-91 -0.9329593375385521 1104 92-93 -1.0947206681210453 1104 94-95 -1.1916294683274167 1104 96-97 -1.3759615675086927 1104 98-99 -1.372201977564501 1104 100 -1.2937694060639835 1105 1 -0.30662703521392487 1105 2 -0.3117801794777293 1105 3 -0.34799336856467633 1105 4 -0.21486900185492175 1105 5 -0.25858628844468257 1105 6 -0.3285432897030347 1105 7 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1105 70-71 -1.0821036352263533 1105 72-73 -1.1013889267246526 1105 74-75 -1.1196707111942672 1105 76-77 -1.0161549802036234 1105 78-79 -0.8395691484066141 1105 80-81 -0.7622015645727451 1105 82-83 -0.8079213301346648 1105 84-85 -0.7673841604858218 1105 86-87 -0.8513407897633982 1105 88-89 -0.8262610943898601 1105 90-91 -0.707199762384846 1105 92-93 -0.8403898933872753 1105 94-95 -0.846256918079682 1105 96-97 -1.0077723421489146 1105 98-99 -0.9923501119967106 1105 100 -0.9073746093540827 1106 1 -0.3052982831783062 1106 2 -0.35354468300525 1106 3 -0.3825601594272321 1106 4 -0.21462191113410256 1106 5 -0.19885466432971555 1106 6 -0.3612659075169091 1106 7 -0.42096861691673126 1106 8 -0.3631920730579594 1106 9 -0.40690602125678765 1106 10-11 -0.5060799434712777 1106 12-13 -0.5613216356650454 1106 14-15 -0.7422628205152222 1106 16-17 -0.7653657898460793 1106 18-19 -0.6916581179498209 1106 20-21 -0.620782008034567 1106 22-23 -0.6160800103032713 1106 24-25 -0.754609629210087 1106 26-27 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1.033471612559623 2311 40-41 1.0442492258017069 2311 42-43 1.0795545574905887 2311 44-45 1.137787390491809 2311 46-47 1.1099336166474174 2311 48-49 1.1159812418177566 2311 50-51 1.1490171225799273 2311 52-53 1.1709352341578736 2311 54-55 1.094771817311866 2311 56-57 1.202054637563272 2311 58-59 1.2468448495039297 2311 60-61 1.2580723841468355 2311 62-63 1.1934364308564582 2311 64-65 1.2550980805030818 2311 66-67 1.2457536075630387 2311 68-69 1.2084667343569961 2311 70-71 1.261147393628356 2311 72-73 1.2672355162673057 2311 74-75 1.1315975610967897 2311 76-77 1.024010508732907 2311 78-79 1.078868379106268 2311 80-81 1.1120675860086422 2311 82-83 1.0785334763255463 2311 84-85 1.0977830509495057 2311 86-87 1.1664124022942879 2311 88-89 1.264386817631955 2311 90-91 1.260889058184393 2311 92-93 1.4106620217953676 2311 94-95 1.4518803191050047 2311 96-97 1.56877476816069 2311 98-99 1.5367457183956041 2311 100 1.6016851280301374 2312 1 0.10605909813342151 2312 2 0.12061350714797214 2312 3 0.3187355944484196 2312 4 0.25794204168563084 2312 5 0.36858448042719516 2312 6 0.4197563875299508 2312 7 0.45024393214273317 2312 8 0.29255706147166194 2312 9 0.39647240848232457 2312 10-11 0.23582182016425435 2312 12-13 0.41233531560425973 2312 14-15 0.7725204562437327 2312 16-17 0.8190515590484253 2312 18-19 0.8709647311724993 2312 20-21 0.8760737504245526 2312 22-23 0.44072834198426847 2312 24-25 0.46905198645284685 2312 26-27 0.7518956981459581 2312 28-29 0.9426694711172914 2312 30-31 0.8596124830963134 2312 32-33 0.7601612166166518 2312 34-35 0.9139788172970427 2312 36-37 0.9981517447960755 2312 38-39 1.0313213364883609 2312 40-41 1.0590071736494053 2312 42-43 0.8974667181750675 2312 44-45 0.8607915090200251 2312 46-47 0.998103520802708 2312 48-49 1.0657244473271135 2312 50-51 1.0485763888895931 2312 52-53 1.0474205342558633 2312 54-55 1.0905817727016327 2312 56-57 1.1188844448931903 2312 58-59 1.094186253479272 2312 60-61 1.0540938647995048 2312 62-63 1.1040895561704644 2312 64-65 1.1724506106667505 2312 66-67 1.3026738690962176 2312 68-69 1.2760056137250615 2312 70-71 1.077590414798479 2312 72-73 1.228558467770661 2312 74-75 1.1972419906370888 2312 76-77 1.0933998504446976 2312 78-79 1.074880131569273 2312 80-81 0.9713676749273645 2312 82-83 0.9529295668668709 2312 84-85 0.8374743130203122 2312 86-87 1.0780448287702846 2312 88-89 0.7132280837831111 2312 90-91 1.0609047787299097 2312 92-93 1.213531524253817 2312 94-95 1.295028412881095 2312 96-97 1.3832955003566916 2312 98-99 1.4983060945483508 2312 100 1.5714076643203825 2313 1 -0.46341571264477466 2313 2 -0.07420956209787732 2313 3 0.22022298113002847 2313 4 0.05512186647833772 2313 5 0.2019764130218462 2313 6 0.3347823077575356 2313 7 0.06661788963589999 2313 8 0.06596936780313456 2313 9 0.23251038211877528 2313 10-11 0.4250423254913187 2313 12-13 0.5780699833217895 2313 14-15 0.6186007837648333 2313 16-17 0.7515204036831591 2313 18-19 0.6121900972902026 2313 20-21 0.7802540783796417 2313 22-23 0.8109235751716284 2313 24-25 0.6497916049147747 2313 26-27 0.8968749831372094 2313 28-29 0.8449285499040187 2313 30-31 0.8241808786592841 2313 32-33 1.0020535753738002 2313 34-35 1.0096798661131317 2313 36-37 0.871558271204151 2313 38-39 0.647874872716514 2313 40-41 0.8580311490872958 2313 42-43 0.9308518417700853 2313 44-45 1.1241689904910643 2313 46-47 1.1387110236298739 2313 48-49 1.1195233693812696 2313 50-51 1.0834368888930754 2313 52-53 1.1090821905596613 2313 54-55 1.2141540033982992 2313 56-57 1.2195593841053665 2313 58-59 1.0773002795976794 2313 60-61 1.1468771537061215 2313 62-63 1.164640138916944 2313 64-65 1.264114935335634 2313 66-67 1.2687207315841462 2313 68-69 1.2663397439294428 2313 70-71 1.1849118549476074 2313 72-73 1.2851872179644772 2313 74-75 0.9289450809037092 2313 76-77 1.1056780615011945 2313 78-79 0.785302928034298 2313 80-81 0.9310925900269353 2313 82-83 1.0590871316556019 2313 84-85 1.0704260541449706 2313 86-87 1.153345838501206 2313 88-89 1.1019178060535175 2313 90-91 1.2390189541136998 2313 92-93 0.9775317612372554 2313 94-95 1.032297342524764 2313 96-97 1.3010169263288915 2313 98-99 1.488293034814717 2313 100 1.4794801854598418 2314 1 -0.24815502646718102 2314 2 -0.0772231234503451 2314 3 0.1617177482934764 2314 4 -0.1325999109053484 2314 5 0.19138305881043038 2314 6 0.22882487439323995 2314 7 0.11817533803993996 2314 8 0.18067178204660195 2314 9 0.2879751596272655 2314 10-11 0.3878771359044606 2314 12-13 0.47168126719841297 2314 14-15 0.6063072994701315 2314 16-17 0.8125974708507329 2314 18-19 0.8487401067683678 2314 20-21 0.45118872758558837 2314 22-23 0.6754425620572846 2314 24-25 0.8988214634942437 2314 26-27 0.8193494429991262 2314 28-29 0.7747604703897579 2314 30-31 0.8149419494468404 2314 32-33 0.9353804724304524 2314 34-35 0.9416248567054595 2314 36-37 0.8157491594207755 2314 38-39 0.8828743661697871 2314 40-41 0.9413197026457283 2314 42-43 0.7723695602000831 2314 44-45 0.7898762211061197 2314 46-47 0.9262063171841461 2314 48-49 0.7537352740648373 2314 50-51 0.8017945599687906 2314 52-53 0.9440268777461682 2314 54-55 1.0675155153515021 2314 56-57 0.9160666791615242 2314 58-59 0.9431706234802419 2314 60-61 0.9724564708289094 2314 62-63 0.7306382161282343 2314 64-65 0.786041815577029 2314 66-67 0.9027348480491639 2314 68-69 0.9650341980783494 2314 70-71 1.003948168081216 2314 72-73 1.0135225539851191 2314 74-75 1.1048504809528978 2314 76-77 0.8673832727181292 2314 78-79 0.829245530023826 2314 80-81 0.8552724021033242 2314 82-83 0.8948970607025046 2314 84-85 0.7712013317404107 2314 86-87 0.8641940196590241 2314 88-89 0.9125516448369275 2314 90-91 1.0295359033237723 2314 92-93 1.0554726566343113 2314 94-95 1.1124785321993578 2314 96-97 1.1269901226961672 2314 98-99 1.1945792927242884 2314 100 1.152419414997052 2315 1 -0.045111197990514995 2315 2 0.09415872919120716 2315 3 0.13131244149413845 2315 4 -0.045112111805607924 2315 5 0.26971833481137963 2315 6 0.38008429159295076 2315 7 0.4504931774144936 2315 8 0.4148801686848387 2315 9 0.5509801347516401 2315 10-11 0.5071916111472916 2315 12-13 0.6060175382242292 2315 14-15 0.7064022532725502 2315 16-17 0.7336844170720127 2315 18-19 0.8098479958941667 2315 20-21 0.7557025394785057 2315 22-23 0.8403746160729213 2315 24-25 0.8200686561026274 2315 26-27 0.888038474034424 2315 28-29 0.9218599728773214 2315 30-31 0.9019152733037359 2315 32-33 0.9120098493553499 2315 34-35 0.9067224165254046 2315 36-37 0.9942911636851619 2315 38-39 0.9998700070131861 2315 40-41 1.049955191866296 2315 42-43 1.135590171429648 2315 44-45 0.9376382206323015 2315 46-47 0.9700049904843056 2315 48-49 1.0776734877582754 2315 50-51 0.9435238184383437 2315 52-53 1.0258869582958 2315 54-55 0.896710587609256 2315 56-57 1.0129682664631119 2315 58-59 1.106432078111844 2315 60-61 1.1477706143967836 2315 62-63 1.13375330733885 2315 64-65 1.1999262505450474 2315 66-67 1.227150578918625 2315 68-69 1.1726577943825376 2315 70-71 0.8559235293700098 2315 72-73 1.098553304991995 2315 74-75 1.2381732954518299 2315 76-77 1.1456434954141699 2315 78-79 1.0338811140086648 2315 80-81 1.0780751028851228 2315 82-83 1.071348801996038 2315 84-85 0.9374833976015786 2315 86-87 0.862740809519245 2315 88-89 1.0016687734794303 2315 90-91 1.0993864126817456 2315 92-93 1.1414075061960247 2315 94-95 1.1820794798420948 2315 96-97 1.254545393958903 2315 98-99 1.1947603872516517 2315 100 1.1886586000242971 2316 1 0.4266693809077111 2316 2 0.4254656386216453 2316 3 0.44145091021496086 2316 4 0.2754308387079334 2316 5 0.35650806403639734 2316 6 0.4187131431335729 2316 7 0.45464947993550453 2316 8 0.4842745664439363 2316 9 0.4894311151438018 2316 10-11 0.44529487912114973 2316 12-13 0.6310890601663459 2316 14-15 0.7308774633565847 2316 16-17 0.8389115412550154 2316 18-19 0.810226007098656 2316 20-21 0.8451093555382698 2316 22-23 0.8705058485659265 2316 24-25 0.9046980678673364 2316 26-27 0.9309526663780332 2316 28-29 0.8555252389837662 2316 30-31 0.8190913704092466 2316 32-33 0.7175711472078277 2316 34-35 0.6125167209138311 2316 36-37 0.6169806594834881 2316 38-39 0.5564014262475467 2316 40-41 0.7047844168896376 2316 42-43 0.8592487148470127 2316 44-45 0.786984952658436 2316 46-47 0.8521007421182958 2316 48-49 0.5775677921466951 2316 50-51 0.6455693833309724 2316 52-53 0.767825427016632 2316 54-55 0.8698222589444597 2316 56-57 0.8874427295816858 2316 58-59 0.829168679419034 2316 60-61 0.9145122110354365 2316 62-63 0.9849052214378204 2316 64-65 0.9526562225338395 2316 66-67 0.9920244818131181 2316 68-69 0.9292820240744177 2316 70-71 0.7899916899675787 2316 72-73 0.8588756205848966 2316 74-75 0.9172028472725557 2316 76-77 0.7475504515299534 2316 78-79 0.7040521690973662 2316 80-81 0.6960592765545925 2316 82-83 0.6035544042369381 2316 84-85 0.7159798028303364 2316 86-87 0.7192151325818052 2316 88-89 0.6517064485494579 2316 90-91 0.6891200728124645 2316 92-93 0.7817088600709106 2316 94-95 0.7369271922603957 2316 96-97 0.8234265330812178 2316 98-99 0.9748624880919863 2316 100 0.6493256401736893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 28.0 13 213.0 14 753.0 15 2124.0 16 4929.0 17 9578.0 18 14845.0 19 20482.0 20 27306.0 21 34256.0 22 40740.0 23 42285.0 24 43706.0 25 47537.0 26 55444.0 27 67014.0 28 80553.0 29 97556.0 30 118833.0 31 146157.0 32 182941.0 33 236548.0 34 322777.0 35 444586.0 36 657333.0 37 976052.0 38 806062.0 39 107716.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21451513654222 19.320400501171594 13.207389184689058 24.257695177597125 2 16.07407423551283 21.077506827043155 39.0223543436994 23.82606459374462 3 18.568140755867553 26.108530916207716 30.75789441566395 24.56543391226078 4 12.747434838589863 17.2724246864948 37.72178485928641 32.25835561562893 5 12.915987117424581 37.33998399812737 34.550815317423144 15.193213567024896 6 30.185011234926563 36.867318895381146 18.59034907821296 14.357320791479328 7 26.50726348056443 32.964683498370334 22.581703940292456 17.94634908077278 8 26.041020783692286 34.62524820976223 20.748494504677815 18.585236501867662 9 26.834947664461485 15.617035410670407 20.409552341858973 37.13846458300914 10-11 24.82804757196132 25.76602439273697 28.733093260667875 20.672834774633838 12-13 25.34467130609061 23.981067732959747 28.28639968511604 22.387861275833593 14-15 22.834457844034237 25.416106736036316 26.37703705414956 25.372398365779887 16-17 22.136274190795984 27.861600870200665 27.128006543504473 22.874118395498876 18-19 21.76837308238002 27.10792721598302 29.18406754468362 21.93963215695334 20-21 22.654539918809938 26.6189329346732 28.865580681765056 21.860946464751805 22-23 22.514825526276216 26.43517196738711 28.742538199840027 22.307464306496644 24-25 22.125808035915203 26.375918124185233 29.05480352635531 22.44347031354425 26-27 22.166662671057477 26.56860715243104 28.733362828193894 22.531367348317595 28-29 21.935196887581515 27.229684270678934 28.71957307333454 22.115545768405013 30-31 22.692290675882518 26.653854444114376 28.77625112595506 21.877603754048046 32-33 22.504443666278345 26.756064218974636 28.500856473148527 22.238635641598492 34-35 22.58143486148455 26.741236539426584 28.741035549124256 21.93629304996462 36-37 22.471993274810583 26.570526194621507 28.677347439487903 22.28013309108001 38-39 22.3148320923991 26.586136734414495 28.769659266984114 22.32937190620229 40-41 22.35810347093439 26.130594345126724 29.17370712548555 22.337595058453335 42-43 22.03818464593735 26.433487988101874 28.707115775745358 22.821211590215427 44-45 21.61082716626042 26.696529009418384 29.026092532576158 22.666551291745034 46-47 21.62291560655314 26.292017959544705 28.742562675800837 23.342503758101323 48-49 21.688131126274403 26.406145688643758 28.729424882964228 23.17629830211762 50-51 21.60230527701239 26.579409768089317 28.51996721381867 23.29831774107962 52-53 21.45819507782979 26.70263117139054 28.32452149200308 23.514652258776593 54-55 21.143644860821418 26.511951505867614 28.612830626579072 23.731573006731892 56-57 21.561724237886942 26.424590746322657 28.13180681875146 23.881878197038944 58-59 21.19189470536386 26.571394195769628 28.306651926649817 23.930059172216694 60-61 21.529026654038873 26.630075704649542 27.95524216366811 23.885655477643475 62-63 21.485279044217247 26.478072707610167 27.850752878557156 24.185895369615434 64-65 21.074062738963743 26.242012016960814 27.806802199708947 24.877123044366495 66-67 21.207054816772306 26.357511496019725 27.512248248614917 24.92318543859305 68-69 21.1245164173601 26.468192492575426 27.40469348413585 25.00259760592862 70-71 21.175139887871662 26.338701720627633 27.266843099364678 25.219315292136024 72-73 21.2246171865325 26.626913631281603 26.87159549362399 25.276873688561906 74-75 21.418812716503417 26.73933042809089 26.61993063170493 25.221926223700763 76-77 21.433221901067377 26.613138194431045 26.627275693105318 25.326364211396264 78-79 21.41041073496356 26.71824535113412 26.608541652475992 25.26280226142633 80-81 21.458944651427316 26.74800150949959 26.370114436240804 25.422939402832284 82-83 21.65300028252726 26.54611734298809 26.52712959281195 25.273752781672698 84-85 21.823032245579892 26.62710307628992 26.133850091168508 25.416014586961676 86-87 21.582304708226825 26.69102129960156 26.284001156061926 25.442672836109686 88-89 21.59595829197363 26.249371972164408 26.31560928294922 25.839060452912744 90-91 21.8454511335199 26.01858512411757 26.28036662709331 25.855597115269212 92-93 21.78641277459434 26.037416955032576 26.299646723375936 25.87652354699715 94-95 21.460420627382902 25.834969384991695 26.590860669427112 26.113749318198288 96-97 21.51765673458432 25.797209066735306 26.534767555359934 26.15036664332044 98-99 21.496167087955826 25.863771751997934 26.370059916397597 26.270001243648643 100 21.40947596914906 25.768297962409846 26.552040140573972 26.270185927867118 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2164.0 1 1537.0 2 1836.0 3 2666.0 4 1855.0 5 1288.0 6 1786.0 7 2880.5 8 4270.5 9 4866.5 10 4139.5 11 3390.5 12 3470.5 13 3843.0 14 4271.5 15 4736.5 16 5151.0 17 5592.0 18 6245.5 19 6927.5 20 7716.0 21 8692.0 22 9842.0 23 11682.5 24 13893.5 25 16675.5 26 20496.5 27 25061.0 28 30263.0 29 36701.5 30 44381.0 31 52190.5 32 61678.0 33 69903.0 34 76983.5 35 85222.0 36 93774.0 37 104602.5 38 112442.0 39 116147.5 40 119472.0 41 123396.5 42 129185.5 43 137605.0 44 147254.0 45 159384.0 46 171007.0 47 179917.5 48 189725.5 49 197314.5 50 201480.0 51 204285.0 52 203116.0 53 196894.0 54 187055.5 55 170269.5 56 151636.5 57 132494.5 58 112696.5 59 95555.5 60 76720.0 61 59201.0 62 45763.0 63 34288.5 64 24837.5 65 17521.5 66 12448.0 67 9476.5 68 7243.0 69 5287.5 70 3973.0 71 3104.0 72 2482.5 73 1889.0 74 1401.5 75 983.0 76 657.5 77 415.0 78 261.5 79 166.0 80 98.5 81 67.5 82 49.5 83 33.5 84 21.5 85 16.5 86 11.5 87 6.0 88 5.5 89 4.0 90 3.5 91 2.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05047544116974002 2 0.0 3 0.0 4 0.014100868064258115 5 0.0034216944143562965 6 0.0 7 1.0897115969287569E-4 8 2.8332501520147676E-4 9 0.0020050693383489126 10-11 8.0638658172728E-4 12-13 2.8332501520147676E-4 14-15 8.717692775430056E-5 16-17 6.538269581572541E-5 18-19 0.0 20-21 1.089711596928757E-5 22-23 0.0 24-25 1.4166250760073838E-4 26-27 0.0 28-29 8.935635094815806E-4 30-31 9.37151973358731E-4 32-33 0.014395090195428877 34-35 0.023624947421415448 36-37 0.0312965170637939 38-39 0.021053228052663582 40-41 1.9614808744717623E-4 42-43 0.001874303946717462 44-45 0.009349725501648734 46-47 0.01339255552625442 48-49 0.005295998361073758 50-51 0.010058038039652425 52-53 0.015223271009094734 54-55 0.009447799545372322 56-57 0.003051192471400519 58-59 0.008848458167061505 60-61 0.0017108472071781483 62-63 0.0010243289011130315 64-65 0.018612274075543167 66-67 0.04358846387715028 68-69 0.05266576147956682 70-71 0.0459313438105471 72-73 0.03927320595331239 74-75 0.02746073224260467 76-77 0.02786392553346831 78-79 0.012150284305755639 80-81 0.0020595549181953504 82-83 0.026196666790167314 84-85 0.045386488012082726 86-87 0.04599672650636283 88-89 0.056359883793155305 90-91 0.03333427775005067 92-93 0.025531942716040774 94-95 0.007464524438961984 96-97 0.004173595416237139 98-99 0.023134577202797508 100 0.019440454889209022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4588370.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.7739152524493 #Duplication Level Percentage of deduplicated Percentage of total 1 80.67402393309608 55.48268485048817 2 12.625934169376851 17.36669853095455 3 3.3687150511369675 6.950391703096328 4 1.278595985541751 3.517362078070811 5 0.6037031513516027 2.0759514684345852 6 0.34819925600868523 1.436821567422433 7 0.2255664922756384 1.0859163576489912 8 0.16345373086291864 0.8993082427250421 9 0.12752358853860832 0.7893266823758217 >10 0.5531972242310077 6.44031269364275 >50 0.020809600083002613 0.9856654560776781 >100 0.009118562068273805 1.1478093903073368 >500 7.377081005396513E-4 0.3576580787668354 >1k 3.161604959557422E-4 0.5262191623018606 >5k 3.5128943995070806E-5 0.21171013815943002 >10k+ 7.025788799014161E-5 0.7261635995273295 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16399 0.35740360956069367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13658 0.2976656198170592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8763 0.19098285447773392 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.538269581572541E-5 2 0.0 0.0 0.0 0.0 6.538269581572541E-5 3 0.0 0.0 0.0 0.0 6.538269581572541E-5 4 0.0 0.0 0.0 2.1794231938575135E-5 6.538269581572541E-5 5 0.0 0.0 0.0 2.1794231938575135E-5 6.538269581572541E-5 6 0.0 0.0 0.0 2.1794231938575135E-5 1.0897115969287569E-4 7 0.0 0.0 0.0 2.1794231938575135E-5 1.0897115969287569E-4 8 0.0 0.0 0.0 2.1794231938575135E-5 1.3076539163145082E-4 9 0.0 0.0 0.0 6.538269581572541E-5 2.1794231938575137E-4 10-11 0.0 0.0 0.0 7.627981178501297E-5 2.1794231938575137E-4 12-13 0.0 0.0 0.0 1.0897115969287569E-4 2.3973655132432652E-4 14-15 0.0 0.0 0.0 1.4166250760073838E-4 3.2691347907862705E-4 16-17 0.0 0.0 0.0 1.9614808744717623E-4 3.9229617489435246E-4 18-19 0.0 0.0 0.0 3.2691347907862705E-4 3.9229617489435246E-4 20-21 0.0 0.0 0.0 8.717692775430054E-4 4.0319329086364E-4 22-23 0.0 0.0 0.0 0.002800558804106905 4.249875228022152E-4 24-25 0.0 0.0 0.0 0.007878614845794912 4.5767887071007787E-4 26-27 0.0 0.0 0.0 0.016825147056580003 4.6857598667936543E-4 28-29 0.0 0.0 0.0 0.04798000161277316 4.79473102648653E-4 30-31 0.0 0.0 0.0 0.13799017951908849 4.903702186179405E-4 32-33 0.0 1.0897115969287568E-5 0.0 0.32387318372319585 5.230615665258033E-4 34-35 0.0 2.1794231938575135E-5 0.0 0.6022618053905853 5.448557984643784E-4 36-37 0.0 2.1794231938575135E-5 0.0 1.0292652946471188 5.448557984643784E-4 38-39 0.0 2.1794231938575135E-5 0.0 1.6076733131809335 5.448557984643784E-4 40-41 0.0 2.1794231938575135E-5 0.0 2.2255397886395385 5.448557984643784E-4 42-43 0.0 2.1794231938575135E-5 0.0 2.8051137985820676 5.448557984643784E-4 44-45 0.0 2.1794231938575135E-5 0.0 3.4880796448411964 5.55752914433666E-4 46-47 0.0 2.1794231938575135E-5 0.0 4.27758659393205 5.884442623415287E-4 48-49 0.0 2.1794231938575135E-5 0.0 5.0718664798174515 6.429298421879665E-4 50-51 0.0 2.1794231938575135E-5 0.0 5.8478501079904195 6.538269581572541E-4 52-53 0.0 2.1794231938575135E-5 0.0 6.576736400944126 6.865183060651168E-4 54-55 0.0 2.1794231938575135E-5 0.0 7.369665044449336 7.410038859115547E-4 56-57 0.0 2.1794231938575135E-5 0.0 8.260613246098288 7.519010018808422E-4 58-59 0.0 2.1794231938575135E-5 0.0 9.19577104723464 7.627981178501298E-4 60-61 0.0 2.1794231938575135E-5 0.0 10.105015506596025 7.627981178501298E-4 62-63 0.0 2.1794231938575135E-5 0.0 11.019806597985777 8.499750456044304E-4 64-65 0.0 2.1794231938575135E-5 0.0 11.91026442941611 8.499750456044304E-4 66-67 0.0 2.1794231938575135E-5 0.0 12.866257516285739 8.499750456044304E-4 68-69 0.0 2.1794231938575135E-5 0.0 13.886587175838041 8.826663935122931E-4 70-71 0.0 2.1794231938575135E-5 0.0 14.923938130534374 9.262548573894433E-4 72-73 0.0 2.1794231938575135E-5 0.0 15.899142832857855 0.0010134317851437438 74-75 0.0 2.1794231938575135E-5 0.0 16.844380466265797 0.0010352260170823188 76-77 0.0 3.2691347907862705E-5 0.0 17.851012451044706 0.0010461231330516066 78-79 0.0 4.358846387715027E-5 0.0 18.90128738528061 0.0010570202490208943 80-81 0.0 4.358846387715027E-5 0.0 19.943759984482504 0.0010679173649901818 82-83 0.0 4.358846387715027E-5 0.0 20.97340667818855 0.001111505828867332 84-85 0.0 4.358846387715027E-5 0.0 21.958396990652453 0.001133300060805907 86-87 0.0 4.358846387715027E-5 0.0 22.95063388523593 0.0011550942927444823 88 0.0 4.358846387715027E-5 0.0 23.712298703025258 0.0011768885246830575 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7480 0.0 53.42926 1 TACCTGG 4125 0.0 45.112934 2 GTATCAA 13445 0.0 40.146053 1 ACCTGGG 5485 0.0 33.24183 3 ATCAACG 16540 0.0 32.13342 3 CAACGCA 16795 0.0 31.697002 5 TATCAAC 17010 0.0 31.466558 2 TCAACGC 17190 0.0 31.000717 4 AACGCAG 18080 0.0 29.963957 6 GTGGTAT 3070 0.0 28.180017 1 CCTGGGG 5130 0.0 26.565329 4 ACGCAGA 20330 0.0 26.185497 7 GTACCTG 7290 0.0 25.927483 1 CGCAGAG 20580 0.0 25.77608 8 CCGCGTA 110 1.02113074E-4 25.628439 9 GTACATG 13940 0.0 25.330221 1 TACATGG 13800 0.0 25.06274 2 ACATGGG 13720 0.0 24.249844 3 ATAACGC 495 0.0 23.733654 3 TGGGGGG 7990 0.0 23.404783 6 >>END_MODULE