FastQCFastQC Report
Mon 6 Jun 2016
ERR1211386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6624886
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280540.42346389054845623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226040.34119832401644345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131260.1981317112475596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120650.055.2584881
TACCTGG57850.045.5549352
GTATCAA222550.041.7069551
ACCTGGG68650.037.7966883
ATCAACG267150.034.187933
CAACGCA268450.033.8992925
TATCAAC275950.033.3230632
TCAACGC284150.032.2079964
AACGCAG287700.031.9744366
GTACATG256650.029.1343631
GTGGTAT45000.028.6885071
ACGCAGA317600.028.4764847
TACATGG261300.028.1674862
CGCAGAG324600.027.759768
ACATGGG261700.027.0685353
CCTGGGG66950.026.8901714
CATGGGG190200.026.8141844
GTACTAG26000.026.4575331
ATGGGGG153250.025.642155
GTACCTG105550.025.533341
Produced by FastQC (version 0.11.5)