##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6624886 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28285437666399 31.0 31.0 34.0 28.0 34.0 2 31.328788450095594 31.0 31.0 34.0 28.0 34.0 3 31.38550021841885 31.0 31.0 34.0 28.0 34.0 4 35.025481947915786 37.0 35.0 37.0 32.0 37.0 5 35.08085120257164 37.0 35.0 37.0 32.0 37.0 6 35.14667331634084 37.0 35.0 37.0 32.0 37.0 7 35.07713702545221 37.0 35.0 37.0 32.0 37.0 8 35.08153951630262 37.0 35.0 37.0 32.0 37.0 9 36.633247726828806 39.0 37.0 39.0 32.0 39.0 10-11 36.55925981216885 39.0 37.0 39.0 32.0 39.0 12-13 36.465805675750495 39.0 37.0 39.0 32.0 39.0 14-15 37.626764973767095 40.0 37.0 41.0 32.0 41.0 16-17 37.61175497963286 40.0 37.0 41.0 32.0 41.0 18-19 37.6197054560637 40.0 37.0 41.0 32.0 41.0 20-21 37.606453756336336 40.0 37.0 41.0 32.0 41.0 22-23 37.48120601622428 40.0 37.0 41.0 31.5 41.0 24-25 37.38303851568163 40.0 37.0 41.0 31.0 41.0 26-27 37.23467996279483 40.0 36.0 41.0 31.0 41.0 28-29 37.09550081314607 39.5 36.0 41.0 31.0 41.0 30-31 36.930304008250104 39.0 36.0 41.0 30.0 41.0 32-33 36.71428172499874 39.0 35.0 41.0 30.0 41.0 34-35 36.58439020686545 39.0 35.0 41.0 30.0 41.0 36-37 36.44783314007215 39.0 35.0 41.0 30.0 41.0 38-39 36.29052031989683 39.0 35.0 40.0 29.0 41.0 40-41 36.115000544915034 39.0 35.0 40.0 28.5 41.0 42-43 35.910073018614966 38.0 35.0 40.0 27.5 41.0 44-45 35.757128575495486 38.0 35.0 40.0 27.0 41.0 46-47 35.50948348394222 38.0 34.0 40.0 26.0 41.0 48-49 35.245837966117456 38.0 34.0 40.0 25.5 41.0 50-51 34.57036928635451 37.5 33.5 39.5 24.5 40.5 52-53 34.870567810525344 38.0 33.5 39.5 25.5 40.5 54-55 35.27951809283964 38.0 34.0 40.0 26.0 41.0 56-57 35.192338253065785 38.0 34.0 40.0 26.0 41.0 58-59 34.982329582727914 38.0 34.0 40.0 25.5 41.0 60-61 34.756649321965696 37.5 34.0 40.0 25.0 41.0 62-63 34.458188186181616 37.0 33.0 40.0 25.0 41.0 64-65 34.240597121218386 36.5 33.0 40.0 24.5 41.0 66-67 34.03281890435548 36.0 33.0 39.5 24.0 41.0 68-69 33.69158714580145 36.0 33.0 39.0 23.0 41.0 70-71 33.32137164322526 35.0 32.0 39.0 23.0 40.5 72-73 32.93383584260921 35.0 32.0 38.0 22.0 40.0 74-75 32.55467241247623 35.0 31.5 37.0 21.5 39.5 76-77 32.163483341449194 35.0 31.0 37.0 20.0 39.0 78-79 31.753568514235567 35.0 31.0 36.0 20.0 39.0 80-81 31.35187473414637 35.0 30.5 36.0 19.0 37.5 82-83 30.97947066560843 34.0 30.0 35.0 18.0 37.0 84-85 30.64000422044998 34.0 30.0 35.0 17.5 37.0 86-87 30.295556180136536 34.0 29.0 35.0 15.5 36.0 88-89 29.962911210849516 34.0 29.0 35.0 13.0 36.0 90-91 29.646884640731933 34.0 29.0 35.0 10.5 36.0 92-93 29.341197720232472 34.0 27.5 35.0 10.0 35.0 94-95 29.047039677361994 34.0 27.0 35.0 10.0 35.0 96-97 28.737468086243297 34.0 26.5 35.0 10.0 35.0 98-99 28.400607648191986 33.5 25.0 35.0 8.0 35.0 100 27.115846974574357 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33451688998019335 1101 2 -0.2763897414493002 1101 3 -0.45331299953797455 1101 4 -0.23768950222024898 1101 5 -0.2780780298728658 1101 6 -0.20394710339162714 1101 7 -0.32984753415343704 1101 8 -0.2506575246386902 1101 9 -0.4952613615344035 1101 10-11 -0.3801688729813577 1101 12-13 -0.47810346742497245 1101 14-15 -0.5843881293383575 1101 16-17 -0.5611935886002826 1101 18-19 -0.5955844588762673 1101 20-21 -0.6752468827141271 1101 22-23 -0.6833861786190027 1101 24-25 -0.6991032228442364 1101 26-27 -0.7353762372103319 1101 28-29 -0.7277261555763843 1101 30-31 -0.7743492650845312 1101 32-33 -0.740225495087067 1101 34-35 -0.7512011688979428 1101 36-37 -0.7788916431682722 1101 38-39 -0.8202726822054842 1101 40-41 -1.000464016913142 1101 42-43 -0.9761359214861969 1101 44-45 -0.8720480077382788 1101 46-47 -0.9481357089221873 1101 48-49 -1.0399777943040647 1101 50-51 -0.9247542718808646 1101 52-53 -0.6944740452241192 1101 54-55 -0.7642336168281645 1101 56-57 -0.7423163521287393 1101 58-59 -0.6249998627433229 1101 60-61 -0.7681723242838316 1101 62-63 -0.8131700250974276 1101 64-65 -0.8001781033988422 1101 66-67 -0.798269154732516 1101 68-69 -0.8876601797466961 1101 70-71 -0.9440147468355562 1101 72-73 -0.9795459807892399 1101 74-75 -0.9981757945588683 1101 76-77 -0.8639165410255565 1101 78-79 -0.9378226190133816 1101 80-81 -1.051851887504366 1101 82-83 -1.0933187157288273 1101 84-85 -1.0695683263627735 1101 86-87 -1.2163174425929455 1101 88-89 -1.2915097401275908 1101 90-91 -1.3526842493855717 1101 92-93 -1.4585195481622861 1101 94-95 -1.5095224122268789 1101 96-97 -1.7477943932114748 1101 98-99 -1.8829251627281884 1101 100 -1.8757790527151386 1102 1 -0.44228362042225555 1102 2 -0.42304063872144226 1102 3 -0.42554801143623777 1102 4 -0.38597044830442684 1102 5 -0.31137919947125425 1102 6 -0.27153549900755536 1102 7 -0.4400954433450437 1102 8 -0.33771469636764095 1102 9 -0.5486465366694162 1102 10-11 -0.4715807913841772 1102 12-13 -0.5417159760152259 1102 14-15 -0.7373529676297395 1102 16-17 -0.6785766464274374 1102 18-19 -0.6483608086269115 1102 20-21 -0.8494245764738437 1102 22-23 -0.7555915000664442 1102 24-25 -0.8230942002154507 1102 26-27 -0.8048250890323487 1102 28-29 -0.853702053864346 1102 30-31 -0.8662595115914655 1102 32-33 -0.8279199239262667 1102 34-35 -0.8379477171207981 1102 36-37 -0.8333780094104242 1102 38-39 -0.9071281543024199 1102 40-41 -1.1031074883705543 1102 42-43 -1.032600114515425 1102 44-45 -1.051854272301945 1102 46-47 -1.1578824026713121 1102 48-49 -1.1867573520367003 1102 50-51 -1.0159965108478062 1102 52-53 -0.805517683893207 1102 54-55 -0.9400284961142376 1102 56-57 -0.858457440400251 1102 58-59 -0.7977057592293164 1102 60-61 -0.8732647886729481 1102 62-63 -0.852503744439737 1102 64-65 -0.8746339201655857 1102 66-67 -0.9457322332380826 1102 68-69 -0.8231771082723185 1102 70-71 -0.8260188058922822 1102 72-73 -0.8874757161125046 1102 74-75 -1.0894816598638144 1102 76-77 -1.0486722721990063 1102 78-79 -1.0956839801764602 1102 80-81 -1.193018442873246 1102 82-83 -1.1831960662320533 1102 84-85 -1.311642527315339 1102 86-87 -1.3335367833655667 1102 88-89 -1.4785125161681805 1102 90-91 -1.5378099242388075 1102 92-93 -1.5964207888234299 1102 94-95 -1.6407761773602871 1102 96-97 -1.7327656589499547 1102 98-99 -1.819622366724662 1102 100 -1.6928448014400708 1103 1 -0.469137696169625 1103 2 -0.43554471861898136 1103 3 -0.5872305066172459 1103 4 -0.3955781729765704 1103 5 -0.30352264784199434 1103 6 -0.4098239361066973 1103 7 -0.5125168902296622 1103 8 -0.5075344996529196 1103 9 -0.5093991624369067 1103 10-11 -0.5542812652309976 1103 12-13 -0.6769954576772648 1103 14-15 -0.7815030807425316 1103 16-17 -0.8543471476852105 1103 18-19 -0.8895214439897288 1103 20-21 -0.8659964836965628 1103 22-23 -0.8455514633333081 1103 24-25 -0.7881718480648203 1103 26-27 -0.9148996498832531 1103 28-29 -0.955981984935157 1103 30-31 -0.8598615543345716 1103 32-33 -0.9331303540458435 1103 34-35 -0.8367668203336862 1103 36-37 -0.9739524292217112 1103 38-39 -0.9586091860444341 1103 40-41 -0.9912143762296779 1103 42-43 -1.0639340583039427 1103 44-45 -1.0953200208387273 1103 46-47 -1.0878660698535256 1103 48-49 -0.9731515261910317 1103 50-51 -0.9752781413385563 1103 52-53 -0.8280661656422197 1103 54-55 -0.824323759332529 1103 56-57 -0.8195630785789021 1103 58-59 -0.8174556875520906 1103 60-61 -0.9204014529222704 1103 62-63 -0.9117716347284457 1103 64-65 -0.8253641350186953 1103 66-67 -0.8939420056136953 1103 68-69 -0.8926918658445615 1103 70-71 -0.8741032299264901 1103 72-73 -0.9200127956433128 1103 74-75 -1.0002079626449571 1103 76-77 -0.9872186811781845 1103 78-79 -1.0113531180953501 1103 80-81 -1.1124272801875996 1103 82-83 -1.0839669229824267 1103 84-85 -1.202737619764907 1103 86-87 -1.2563967096942719 1103 88-89 -1.2812760494404714 1103 90-91 -1.2737660653816043 1103 92-93 -1.327432606719956 1103 94-95 -1.287433902148173 1103 96-97 -1.4156650267683872 1103 98-99 -1.3663196255956898 1103 100 -1.3853246946137219 1104 1 -0.5474185573962238 1104 2 -0.5124584350675825 1104 3 -0.5135789562215116 1104 4 -0.37817534162024913 1104 5 -0.39305577613480835 1104 6 -0.362985098570185 1104 7 -0.2836907583027539 1104 8 -0.42798549762329685 1104 9 -0.5348194436711395 1104 10-11 -0.5125708417376273 1104 12-13 -0.5909050169356576 1104 14-15 -0.7569960019718778 1104 16-17 -0.7482858226316438 1104 18-19 -0.8065800296529559 1104 20-21 -0.8331304876522765 1104 22-23 -0.7699545350232029 1104 24-25 -0.7479690714279386 1104 26-27 -0.7799436525241532 1104 28-29 -0.921168955936075 1104 30-31 -0.9722271182505224 1104 32-33 -0.9715205765445987 1104 34-35 -0.9573775378436338 1104 36-37 -1.0665736730057418 1104 38-39 -1.051020538145714 1104 40-41 -1.0771352295923862 1104 42-43 -1.0593727940527558 1104 44-45 -1.1664983846527477 1104 46-47 -1.123525597761116 1104 48-49 -1.0969895453778875 1104 50-51 -1.0721947033714017 1104 52-53 -0.9715750862829253 1104 54-55 -0.9449093663522419 1104 56-57 -0.8414427942570626 1104 58-59 -0.966124196685719 1104 60-61 -0.9943656974291528 1104 62-63 -1.088181863464257 1104 64-65 -1.0568334478833563 1104 66-67 -0.9629529447796727 1104 68-69 -1.0309580796921765 1104 70-71 -0.9320910301623542 1104 72-73 -1.023188598431517 1104 74-75 -1.0650538976459316 1104 76-77 -1.0030781873477714 1104 78-79 -0.9694521343339026 1104 80-81 -1.0312740556235234 1104 82-83 -1.127486604099012 1104 84-85 -1.0899498984500546 1104 86-87 -1.1624273133992986 1104 88-89 -1.1869200112859808 1104 90-91 -1.1912616902415678 1104 92-93 -1.2362265581495642 1104 94-95 -1.280131334768086 1104 96-97 -1.2096294118180424 1104 98-99 -1.351691754378674 1104 100 -1.2995997600515778 1105 1 -0.49727601922375797 1105 2 -0.5184473858767262 1105 3 -0.5593666938098352 1105 4 -0.3788071105949129 1105 5 -0.5479734088858521 1105 6 -0.49363850103945595 1105 7 -0.4982368753774651 1105 8 -0.5149511037672525 1105 9 -0.618012434855963 1105 10-11 -0.6026676031792135 1105 12-13 -0.7018747999916215 1105 14-15 -0.8294336334957038 1105 16-17 -0.7420028678160051 1105 18-19 -0.7448349477828771 1105 20-21 -0.8307309991503828 1105 22-23 -0.8539516405253806 1105 24-25 -0.7790719356626425 1105 26-27 -0.8558670599940399 1105 28-29 -0.9717919341683441 1105 30-31 -0.9360227654066833 1105 32-33 -0.9439469168895513 1105 34-35 -0.899257543851057 1105 36-37 -0.9531167333001846 1105 38-39 -0.9997011198942616 1105 40-41 -0.98603975580248 1105 42-43 -0.8732072026269506 1105 44-45 -0.896592643796815 1105 46-47 -0.986133472453119 1105 48-49 -1.0554341521225012 1105 50-51 -1.1324578811568458 1105 52-53 -0.9092019442195536 1105 54-55 -0.8763144045469744 1105 56-57 -0.8817269262585157 1105 58-59 -0.7911461509433835 1105 60-61 -0.7597019271745182 1105 62-63 -0.9290585390923454 1105 64-65 -0.8457209312460137 1105 66-67 -0.7631144721786782 1105 68-69 -0.8663633057984939 1105 70-71 -0.7516393814122964 1105 72-73 -0.8130677354940659 1105 74-75 -0.8592604514587663 1105 76-77 -0.9607076597758777 1105 78-79 -1.0957948428019115 1105 80-81 -1.0130718351561683 1105 82-83 -0.9668144181807499 1105 84-85 -0.9043292719042135 1105 86-87 -0.9876068310466195 1105 88-89 -0.9764481301365002 1105 90-91 -1.0071106982276241 1105 92-93 -0.9683728767267574 1105 94-95 -1.0939934860904614 1105 96-97 -1.1295060952359073 1105 98-99 -1.1151882424551545 1105 100 -1.2086514544289564 1106 1 -0.5485776901272885 1106 2 -0.5661466672725055 1106 3 -0.6464530529458123 1106 4 -0.4319053310802303 1106 5 -0.44855688934232774 1106 6 -0.43730700656971067 1106 7 -0.4885899068948447 1106 8 -0.47671189098885947 1106 9 -0.6972847557666242 1106 10-11 -0.8130189905398879 1106 12-13 -0.7405081107314615 1106 14-15 -0.8784519876245795 1106 16-17 -0.8407925233378464 1106 18-19 -0.9195858923922842 1106 20-21 -0.8972180108588717 1106 22-23 -0.8647229748639376 1106 24-25 -0.8945258142564185 1106 26-27 -0.9557817449678367 1106 28-29 -0.9570209895558222 1106 30-31 -1.0258945883236734 1106 32-33 -1.0138481300835807 1106 34-35 -0.952958465656188 1106 36-37 -0.9912749092949014 1106 38-39 -1.1316277155577197 1106 40-41 -1.065446201177835 1106 42-43 -0.9693241666548928 1106 44-45 -1.0137568435257904 1106 46-47 -1.0080536882264326 1106 48-49 -0.9648602836590996 1106 50-51 -0.9926680240579557 1106 52-53 -0.935461765635722 1106 54-55 -0.9220771956724008 1106 56-57 -0.9637112860031323 1106 58-59 -0.9002132301950212 1106 60-61 -0.8129907948907871 1106 62-63 -0.9564266048848467 1106 64-65 -0.9255775289283648 1106 66-67 -0.9392432763208873 1106 68-69 -0.861532482965977 1106 70-71 -0.9354857681476219 1106 72-73 -0.8572936772890429 1106 74-75 -0.8741688385160558 1106 76-77 -0.9640878617245399 1106 78-79 -0.9352447747950308 1106 80-81 -0.9420539460129262 1106 82-83 -0.9399076296872266 1106 84-85 -0.9134172583337552 1106 86-87 -0.9049686340541641 1106 88-89 -0.8738315944792987 1106 90-91 -0.927507438081907 1106 92-93 -0.9449718269334788 1106 94-95 -0.9773963096368448 1106 96-97 -0.9342404312081634 1106 98-99 -0.9823678610326567 1106 100 -0.9778570550543897 1107 1 -0.547612159826965 1107 2 -0.5156224584535813 1107 3 -0.5360602590629604 1107 4 -0.4328295764997918 1107 5 -0.43263690341094474 1107 6 -0.4913398347984099 1107 7 -0.48554410489236943 1107 8 -0.4307587421830661 1107 9 -0.6145028633947192 1107 10-11 -0.5982645929061405 1107 12-13 -0.7638969632975972 1107 14-15 -0.84704448977417 1107 16-17 -0.8407941360285065 1107 18-19 -0.8605124630636638 1107 20-21 -0.897870679172172 1107 22-23 -0.8536181855001956 1107 24-25 -0.8936794493901701 1107 26-27 -1.005955429270287 1107 28-29 -1.0017515699815434 1107 30-31 -0.8969678106324324 1107 32-33 -0.9418212716883474 1107 34-35 -0.961275706817851 1107 36-37 -0.8550287986085507 1107 38-39 -0.8488520109558024 1107 40-41 -0.7948048276536639 1107 42-43 -0.8676446163266363 1107 44-45 -1.0193568746085404 1107 46-47 -0.8697054476768997 1107 48-49 -0.9376169267536909 1107 50-51 -1.002012871704828 1107 52-53 -0.9085405250806318 1107 54-55 -0.7728198967833606 1107 56-57 -0.751033488098976 1107 58-59 -0.8020518017460816 1107 60-61 -0.698679237148724 1107 62-63 -0.7308570075077085 1107 64-65 -0.7225894463649567 1107 66-67 -0.7637779679267993 1107 68-69 -0.7110406073541569 1107 70-71 -0.6732005140352086 1107 72-73 -0.6623747544433414 1107 74-75 -0.60014138526126 1107 76-77 -0.6768926652204144 1107 78-79 -0.6844476590203143 1107 80-81 -0.7150751795416497 1107 82-83 -0.7100954153567649 1107 84-85 -0.7615513950946848 1107 86-87 -0.7629286468683567 1107 88-89 -0.6432054923913171 1107 90-91 -0.6985169020453661 1107 92-93 -0.7818428825402464 1107 94-95 -0.8360223388808308 1107 96-97 -0.776091798094992 1107 98-99 -0.7967887740874922 1107 100 -0.8340951608405476 1108 1 -0.4905776097226351 1108 2 -0.46457183246752365 1108 3 -0.5731478653024631 1108 4 -0.3929317127408254 1108 5 -0.46293663240478367 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1.1881945416356245 2311 56-57 1.2640872079963614 2311 58-59 1.2214573304001206 2311 60-61 1.2090768514898542 2311 62-63 1.2630032899162913 2311 64-65 1.2216259614441256 2311 66-67 1.2339164108898615 2311 68-69 1.2480591898589708 2311 70-71 1.2711034162023438 2311 72-73 1.2431102392954614 2311 74-75 1.2783722601591094 2311 76-77 1.2691009222858867 2311 78-79 1.217634299213902 2311 80-81 1.3413277811272408 2311 82-83 1.394530962569661 2311 84-85 1.4204809422854048 2311 86-87 1.459955041093096 2311 88-89 1.5078163780562477 2311 90-91 1.5103727038611048 2311 92-93 1.5799261038110863 2311 94-95 1.6890247093948467 2311 96-97 1.6958062826697677 2311 98-99 1.7771321410605196 2311 100 1.7837114812575017 2312 1 0.5929653862267266 2312 2 0.6366956285624497 2312 3 0.7159414791941714 2312 4 0.5314348573357535 2312 5 0.6073648041557007 2312 6 0.49455294666766747 2312 7 0.6105049270838592 2312 8 0.6529840969746061 2312 9 0.83889374948523 2312 10-11 0.9000289627765312 2312 12-13 0.9516777953086404 2312 14-15 1.1379264954689319 2312 16-17 1.0920970052920609 2312 18-19 1.118560479253233 2312 20-21 1.1606258443135644 2312 22-23 1.2023959997097293 2312 24-25 1.2155194577998287 2312 26-27 1.1804949722853877 2312 28-29 1.260835775480409 2312 30-31 1.1856882505108572 2312 32-33 1.30208358468996 2312 34-35 1.3449317019644624 2312 36-37 1.3613485040760835 2312 38-39 1.3419869062510514 2312 40-41 1.423333321507343 2312 42-43 1.4878546379597495 2312 44-45 1.3468089371320744 2312 46-47 1.3449983866427715 2312 48-49 1.4240140997648538 2312 50-51 1.259554688348949 2312 52-53 1.1321502696985917 2312 54-55 1.1566325832367994 2312 56-57 1.1993972276293832 2312 58-59 1.2368399670970618 2312 60-61 1.1779896194803214 2312 62-63 1.144582637847357 2312 64-65 1.1848149364329643 2312 66-67 1.2388490074470226 2312 68-69 1.205786817080309 2312 70-71 1.1897911174190199 2312 72-73 1.2817727159746184 2312 74-75 1.2400793920047946 2312 76-77 1.178126029859115 2312 78-79 1.1639978023459072 2312 80-81 0.9837589164830192 2312 82-83 1.1289696323212368 2312 84-85 1.2115720813999893 2312 86-87 1.2777006240888404 2312 88-89 1.3039286892075808 2312 90-91 1.3442373792780344 2312 92-93 1.4738829140902006 2312 94-95 1.5001708012721906 2312 96-97 1.4570076590098218 2312 98-99 1.310771579251469 2312 100 1.412225408513759 2313 1 0.6539202556168995 2313 2 0.6691970146722532 2313 3 0.7348601949822822 2313 4 0.6095409138769767 2313 5 0.6208400622715473 2313 6 0.651473739158746 2313 7 0.6874011142419434 2313 8 0.7253010475852832 2313 9 0.8859002044664521 2313 10-11 0.7808463284665592 2313 12-13 0.9192890463202161 2313 14-15 1.1037562512423094 2313 16-17 1.0576232135688528 2313 18-19 1.0784613123660236 2313 20-21 1.107055488715858 2313 22-23 1.1433939927392913 2313 24-25 1.164876813285197 2313 26-27 1.1971876228589196 2313 28-29 1.3091321314836435 2313 30-31 1.28112203606063 2313 32-33 1.3205657185936914 2313 34-35 1.2155311926959698 2313 36-37 1.2350065297372481 2313 38-39 1.2865879809357992 2313 40-41 1.299603302538884 2313 42-43 1.3170008546084588 2313 44-45 1.335404704910303 2313 46-47 1.3173631552784855 2313 48-49 1.3802690471067507 2313 50-51 1.278767154917027 2313 52-53 1.1262263207556629 2313 54-55 1.1103929348495 2313 56-57 1.0925524777551274 2313 58-59 1.0952340551453332 2313 60-61 1.11510725865449 2313 62-63 1.1458975079368585 2313 64-65 1.148019071672259 2313 66-67 1.1060763678377796 2313 68-69 1.0833515221328227 2313 70-71 1.1218464163173465 2313 72-73 1.1539416909486775 2313 74-75 1.1152212936771093 2313 76-77 1.1402797561181544 2313 78-79 1.1063513092790664 2313 80-81 1.206832565485655 2313 82-83 1.2623760943761937 2313 84-85 1.3306553920752755 2313 86-87 1.3677932899753884 2313 88-89 1.4361177961831046 2313 90-91 1.393571584368427 2313 92-93 1.4374157668518812 2313 94-95 1.5570688251890417 2313 96-97 1.5692150310843012 2313 98-99 1.6747699418433264 2313 100 1.7072191155049161 2314 1 0.5893240964519464 2314 2 0.5948872911518706 2314 3 0.671399272673689 2314 4 0.5845725261823915 2314 5 0.600771077388373 2314 6 0.5602712529967562 2314 7 0.6191371780635251 2314 8 0.6055080445190981 2314 9 0.7889179375330855 2314 10-11 0.7528562003086705 2314 12-13 0.531720019633255 2314 14-15 0.9377374470379465 2314 16-17 0.9852728379402791 2314 18-19 1.0228065489167832 2314 20-21 1.0295124111236262 2314 22-23 0.8843389580171674 2314 24-25 0.8882191685465841 2314 26-27 1.0777367380699445 2314 28-29 1.0744750310590518 2314 30-31 1.0165667773279168 2314 32-33 1.0797595706898093 2314 34-35 1.133274282627525 2314 36-37 1.164883814986439 2314 38-39 1.2212952362842273 2314 40-41 1.2224833560631012 2314 42-43 1.2799298406563722 2314 44-45 1.2846577530784415 2314 46-47 1.294580864935007 2314 48-49 1.3980564586533148 2314 50-51 1.3295554300239445 2314 52-53 1.1326754424823022 2314 54-55 1.0777524967382206 2314 56-57 1.0950140956401526 2314 58-59 1.1145995833461555 2314 60-61 1.140596559866374 2314 62-63 1.1585663591632667 2314 64-65 1.0874743232743072 2314 66-67 1.1361277586158494 2314 68-69 1.2110854652054925 2314 70-71 1.1349154306073217 2314 72-73 1.1276604724156343 2314 74-75 1.206545058846352 2314 76-77 1.209647167891518 2314 78-79 1.2167215004751348 2314 80-81 1.288406248056397 2314 82-83 1.0993108952816186 2314 84-85 1.180269855529069 2314 86-87 1.4300671771828348 2314 88-89 1.451855717437855 2314 90-91 1.5357738150949167 2314 92-93 1.6820354764395908 2314 94-95 1.6983220140485145 2314 96-97 1.6753136929982126 2314 98-99 1.7121067221144024 2314 100 1.595350571185513 2315 1 0.48844646557192206 2315 2 0.5427464901844772 2315 3 0.5807648829817076 2315 4 0.4827623055866255 2315 5 0.4632415179570657 2315 6 -0.1749442903139098 2315 7 0.319763794315314 2315 8 0.3719879329504323 2315 9 0.625170819571828 2315 10-11 0.6610329725405464 2315 12-13 0.7063840242545609 2315 14-15 0.8522522410231872 2315 16-17 0.741308455951085 2315 18-19 0.8564856990107401 2315 20-21 0.8963960127345345 2315 22-23 0.8092484739462122 2315 24-25 0.8613934559177068 2315 26-27 0.8949766703865336 2315 28-29 0.9981820900529712 2315 30-31 1.0506164812362613 2315 32-33 1.0681378034955102 2315 34-35 1.1436971254235004 2315 36-37 1.1680057873467575 2315 38-39 1.1859815814119585 2315 40-41 1.1650153501559544 2315 42-43 1.2191901943822998 2315 44-45 1.2262747736588153 2315 46-47 1.2520269040409318 2315 48-49 1.2628043628828962 2315 50-51 1.1566182316491265 2315 52-53 1.0101045960623125 2315 54-55 0.8951910412884345 2315 56-57 0.7989059843964768 2315 58-59 0.8406857500993752 2315 60-61 0.7192563934913281 2315 62-63 0.8914592130458274 2315 64-65 0.9530423338109344 2315 66-67 0.9805663185976741 2315 68-69 0.9978690737141491 2315 70-71 1.0428094139720443 2315 72-73 1.086716465169097 2315 74-75 1.024277175797117 2315 76-77 1.0040714210992974 2315 78-79 0.946014048625937 2315 80-81 0.9433532590747546 2315 82-83 1.1325615951422279 2315 84-85 1.0653521106485044 2315 86-87 1.1589077930267102 2315 88-89 1.2821338712448807 2315 90-91 1.3777497785989787 2315 92-93 1.4887620214642823 2315 94-95 1.5214168937107928 2315 96-97 1.58637561818243 2315 98-99 1.6386471080273175 2315 100 1.6963271130959932 2316 1 0.07729021505841871 2316 2 0.008822592200260715 2316 3 0.13267795486703093 2316 4 0.09222578559052153 2316 5 0.17581934694433698 2316 6 0.17098184489729817 2316 7 0.18453290155088808 2316 8 0.20213498979027378 2316 9 0.27618002428202004 2316 10-11 0.19238105202514078 2316 12-13 0.28102518685230393 2316 14-15 0.510871346873067 2316 16-17 0.501304423939267 2316 18-19 0.41091818047120654 2316 20-21 0.3870513072393962 2316 22-23 0.19985118245750755 2316 24-25 0.360090471076262 2316 26-27 0.4639641412286366 2316 28-29 0.5997584681046035 2316 30-31 0.5587074523992825 2316 32-33 0.6195941163776624 2316 34-35 0.611837548819075 2316 36-37 0.6581152182076835 2316 38-39 0.7173556580530445 2316 40-41 0.7192413718871222 2316 42-43 0.7516747450815373 2316 44-45 0.7019035737502506 2316 46-47 0.6089453145404491 2316 48-49 0.7180163512999371 2316 50-51 0.4750837666546701 2316 52-53 0.25345159261818395 2316 54-55 0.21076023860661053 2316 56-57 0.29226935180324887 2316 58-59 0.36208528801217454 2316 60-61 0.4580863841053784 2316 62-63 0.4603472631585461 2316 64-65 0.4841990828076561 2316 66-67 0.4989943937853738 2316 68-69 0.5129229908357118 2316 70-71 0.35974680362208744 2316 72-73 0.4579777031144445 2316 74-75 0.5801376001295822 2316 76-77 0.6642861024293438 2316 78-79 0.6905110974581596 2316 80-81 0.7618048144772658 2316 82-83 0.8129634976926248 2316 84-85 0.8731526494273112 2316 86-87 0.9518943365551458 2316 88-89 1.0108746658602286 2316 90-91 1.0294183545851112 2316 92-93 1.1180035587153867 2316 94-95 1.1649514407434474 2316 96-97 1.1816504295865506 2316 98-99 1.36147690648313 2316 100 1.2730042239976562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 43.0 1 381.0 2 161.0 3 121.0 4 124.0 5 92.0 6 127.0 7 788.0 8 2514.0 9 4293.0 10 5799.0 11 7684.0 12 10168.0 13 12478.0 14 15023.0 15 18910.0 16 24564.0 17 32511.0 18 40384.0 19 48608.0 20 58436.0 21 69359.0 22 75326.0 23 75351.0 24 75688.0 25 78652.0 26 88102.0 27 100851.0 28 119400.0 29 142070.0 30 171686.0 31 210269.0 32 264066.0 33 337576.0 34 460796.0 35 611832.0 36 867896.0 37 1220751.0 38 1094823.0 39 276918.0 40 265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98431010844126 19.11067065417688 12.56257840974708 23.342440827634782 2 16.325286998376423 20.79670205078745 40.13617494731687 22.741836003519264 3 18.661699159740245 27.310548247107295 30.318742489592438 23.70901010356002 4 12.747863634991091 17.11706978289096 38.26800434821995 31.867062233897997 5 12.81783181406602 37.84620625063509 34.52437770636712 14.811584228931766 6 29.145990781182977 37.16630117621473 19.3162458995596 14.371462143042693 7 26.39083497902452 33.33296077150506 23.14409458035819 17.132109669112232 8 25.819491206600304 34.7571985024632 21.15226180826557 18.27104848267093 9 27.632142646270246 15.554013255225687 20.91297333808708 35.90087076041698 10-11 24.723136453176195 25.939326844296566 29.018099469955366 20.319437232571875 12-13 25.146838725889115 24.412301547811172 28.424383284901783 22.016476441397927 14-15 23.051251668018796 25.66914191836448 26.605851904766688 24.673754508850042 16-17 21.837717865637096 28.050013236746473 27.49179015993779 22.620478737678635 18-19 21.38453175660053 27.627399647732233 29.553745594671337 21.4343230009959 20-21 22.536667571782516 27.06014823215927 29.061496016848594 21.341688179209616 22-23 22.518730398146232 26.683682462896847 28.9299638433203 21.867623295636626 24-25 21.955380272299674 26.57352571277938 29.372162915996192 22.09893109892476 26-27 21.89011995071257 26.76013352566593 29.127495783823154 22.222250739798348 28-29 21.66307359199193 27.597659377124735 29.050988194298238 21.68827883658509 30-31 22.702826500178187 26.815937234076042 29.19884992145277 21.28238634429301 32-33 22.506558167733733 26.985709916891754 28.751411981118878 21.75631993425564 34-35 22.461629934909077 27.082193085907 29.080359530299337 21.37581744888458 36-37 22.172215248942802 26.83305757697618 29.036129052616307 21.958598121464707 38-39 22.11222347887621 26.977724008890284 29.089333000574175 21.820719511659327 40-41 22.006632049440068 26.334313812527064 29.63544412657633 22.023610011456537 42-43 21.78790123065332 26.742751103933998 28.92156840540518 22.547779260007506 44-45 21.163735730984264 27.148301874911425 29.425100299980695 22.262862094123616 46-47 21.211063433747253 26.546531186742012 29.100880625550772 23.14152475395996 48-49 21.53774889490154 26.66786718356847 29.026769639305073 22.767614282224912 50-51 21.464672964026654 26.78970228991843 28.957598739058373 22.788026006996542 52-53 21.108682243251046 27.201718461554375 28.62883164895278 23.0607676462418 54-55 20.95020725865498 26.824537969004865 29.055950209901344 23.169304562438807 56-57 21.488940775520316 26.69020768990483 28.538268118945343 23.28258341562951 58-59 21.041025420693728 26.787039876322005 28.891201315551125 23.280733387433145 60-61 21.550707540493086 26.798017381318463 28.52808673311279 23.12318834507566 62-63 21.600011414449607 26.538293364641536 28.527910443073907 23.33378477783495 64-65 21.232281971944673 26.307248826724898 28.51433318431007 23.946136017020358 66-67 21.388445762457224 26.27650233701483 28.234785622017387 24.100266278510563 68-69 21.030301758236746 26.536488482348098 28.41608447098499 24.017125288430165 70-71 21.286357265556262 26.445118794330657 28.40812025585832 23.860403684254756 72-73 21.499563682595905 26.84636787374982 27.93172110207955 23.722347341574725 74-75 21.90950528077865 26.9705779905124 27.648712376594233 23.471204352114718 76-77 21.92280000066429 26.808446490117877 27.554555834741297 23.714197674476537 78-79 21.803142750059163 26.926611166111776 27.750725335662356 23.519520748166702 80-81 21.99595739868812 27.013751729081697 27.510040827616027 23.480250044614156 82-83 22.528119858085518 26.755713858991225 27.67534680900388 23.040819473919374 84-85 22.703166082421085 27.099519411745305 27.01366584393476 23.183648661898854 86-87 22.464849007606404 27.230608822412034 27.233152618158368 23.071389551823195 88-89 22.775689710226214 26.536589158639202 27.28449645699516 23.403224674139427 90-91 23.483577596544215 26.33773069264807 27.12004430850879 23.05864740229893 92-93 23.62794436274099 26.489922997451366 26.89854846655425 22.983584173253394 94-95 23.390261241400363 26.3275879002346 26.919277700445782 23.36287315791925 96-97 23.65028864904757 26.107700911392982 26.790445441736615 23.451564997822828 98-99 23.75500580747275 26.236066878229547 26.450986779647838 23.55794053464987 100 24.215289631487916 26.17251888687654 26.251214520340906 23.360976961294643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3258.0 1 2256.5 2 2596.5 3 3638.0 4 2575.5 5 1988.0 6 2706.5 7 4225.5 8 6414.0 9 7704.0 10 6802.0 11 5819.0 12 6122.5 13 6808.0 14 7534.5 15 8231.0 16 8893.5 17 9819.0 18 10833.0 19 11697.5 20 12755.0 21 14202.5 22 15976.5 23 18236.5 24 21021.0 25 24633.5 26 29792.0 27 35972.0 28 42680.5 29 51617.5 30 64395.0 31 76540.5 32 91419.5 33 108202.5 34 123915.0 35 139919.5 36 156476.5 37 176477.5 38 186996.5 39 188721.0 40 190435.0 41 192649.0 42 197801.5 43 207568.5 44 220250.5 45 237715.5 46 255724.5 47 270904.0 48 285668.0 49 295270.5 50 298052.0 51 295448.0 52 283219.0 53 265122.0 54 246108.5 55 218607.5 56 192117.5 57 164382.5 58 134842.0 59 111206.5 60 87815.0 61 67557.0 62 52524.0 63 40941.0 64 30308.0 65 20946.0 66 15193.0 67 11559.0 68 8791.0 69 6721.0 70 5157.5 71 3917.0 72 2979.0 73 2329.5 74 1815.0 75 1367.5 76 1019.0 77 703.5 78 481.5 79 351.0 80 240.0 81 177.5 82 144.0 83 122.5 84 93.5 85 65.5 86 48.0 87 35.5 88 32.0 89 28.5 90 17.0 91 14.5 92 15.0 93 8.5 94 8.0 95 6.0 96 2.5 97 5.5 98 6.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22006416412297508 2 0.14892331732198863 3 0.023804183196510852 4 0.022430574654416696 5 0.027879725024702314 6 0.055034909279948356 7 0.06129916801587228 8 0.05453678750094718 9 0.041404486054552485 10-11 0.04372150705687615 12-13 0.04264224320237359 14-15 0.02089847281900398 16-17 0.019313539885818413 18-19 0.018588999116362154 20-21 0.02150225679355086 22-23 0.024581555063739963 24-25 0.021856979878597155 26-27 0.019479580478818804 28-29 0.01908712089536333 30-31 0.017577660958996124 32-33 0.025004203845922786 34-35 0.025494778325242124 36-37 0.02396267648982941 38-39 0.022294723260143645 40-41 0.024060791385693277 42-43 0.029902401339434368 44-45 0.039645965228684694 46-47 0.04446114242569608 48-49 0.041336560357415966 50-51 0.02866464419161326 52-53 0.04402339904414959 54-55 0.042966777088692544 56-57 0.044838507409787884 58-59 0.047917805679976985 60-61 0.02453627126564895 62-63 0.025691008116969862 64-65 0.026551400280699168 66-67 0.03201564525034846 68-69 0.036211943873449295 70-71 0.04083843857841478 72-73 0.04508002099960663 74-75 0.0258872379086976 76-77 0.019004100598863136 78-79 0.018543715318271137 80-81 0.021555087891323713 82-83 0.019917323860365294 84-85 0.012324740380438244 86-87 0.013985146310442172 88-89 0.0191852357912272 90-91 0.02003808065527467 92-93 0.0202116685479569 94-95 0.023660784502555966 96-97 0.024423061770421407 98-99 0.02948729985693339 100 0.028694833390340603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6624886.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.06446240888418 #Duplication Level Percentage of deduplicated Percentage of total 1 77.01259174168845 47.027325134210784 2 14.045851438068357 17.15404734281394 3 4.2973358897586476 7.872435176955472 4 1.7128852942397368 4.183856786433317 5 0.8565856212136234 2.6153470233295 6 0.5126968244497311 1.8784533578258937 7 0.31217956137750885 1.3344153963391188 8 0.2169903694596675 1.0600320207167804 9 0.16799227279361845 0.9232522044290035 >10 0.795349670716776 8.36611998620741 >50 0.04202754378092112 1.7770167213166501 >100 0.02496256014510927 2.8863352932517294 >500 0.0016612602085842678 0.6733330670683357 >1k 7.416267494031065E-4 0.8778439592971403 >5k 5.933013994737002E-5 0.21475113778391658 >10k+ 8.899520992105504E-5 1.1554353920210318 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28054 0.42346389054845623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22604 0.34119832401644345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13126 0.1981317112475596 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.509459936367207E-5 0.0 2 0.0 0.0 0.0 1.509459936367207E-5 0.0 3 0.0 0.0 0.0 3.018919872734414E-5 0.0 4 0.0 0.0 0.0 3.018919872734414E-5 0.0 5 0.0 0.0 0.0 3.018919872734414E-5 0.0 6 0.0 0.0 0.0 3.018919872734414E-5 3.018919872734414E-5 7 0.0 0.0 0.0 3.018919872734414E-5 3.018919872734414E-5 8 0.0 0.0 0.0 3.018919872734414E-5 4.528379809101621E-5 9 0.0 0.0 0.0 9.056759618203241E-5 4.528379809101621E-5 10-11 0.0 0.0 0.0 1.2075679490937656E-4 4.528379809101621E-5 12-13 0.0 1.509459936367207E-5 0.0 1.5094599363672069E-4 4.528379809101621E-5 14-15 0.0 1.509459936367207E-5 0.0 2.1132439109140897E-4 7.547299681836034E-5 16-17 0.0 3.018919872734414E-5 0.0 2.5660818918242517E-4 9.056759618203241E-5 18-19 0.0 3.018919872734414E-5 0.0 3.849122837736378E-4 1.0566219554570449E-4 20-21 1.509459936367207E-5 3.018919872734414E-5 0.0 9.434124602295043E-4 1.2075679490937656E-4 22-23 1.509459936367207E-5 3.018919872734414E-5 0.0 0.00302646717241625 1.2830409459121259E-4 24-25 1.509459936367207E-5 3.018919872734414E-5 0.0 0.008619016236656752 1.3585139427304863E-4 26-27 1.509459936367207E-5 3.018919872734414E-5 0.0 0.01819653953290668 1.3585139427304863E-4 28-29 1.509459936367207E-5 3.018919872734414E-5 0.0 0.04849894775547836 1.4339869395488464E-4 30-31 1.509459936367207E-5 3.018919872734414E-5 0.0 0.14231188280070028 1.5094599363672069E-4 32-33 1.509459936367207E-5 3.018919872734414E-5 0.0 0.33365102433460747 1.5094599363672069E-4 34-35 1.509459936367207E-5 3.018919872734414E-5 0.0 0.6252334002426607 1.8113519236406483E-4 36-37 1.509459936367207E-5 3.018919872734414E-5 0.0 1.056614408157363 1.8113519236406483E-4 38-39 1.509459936367207E-5 3.018919872734414E-5 0.0 1.6561718948824176 1.8113519236406483E-4 40-41 1.509459936367207E-5 3.773649840918017E-5 0.0 2.2816015249168062 2.0377709140957293E-4 42-43 1.509459936367207E-5 4.528379809101621E-5 0.0 2.8705626028885627 2.18871690773245E-4 44-45 1.509459936367207E-5 4.528379809101621E-5 0.0 3.576038893348504 2.2641899045508103E-4 46-47 1.509459936367207E-5 4.528379809101621E-5 0.0 4.373079023548481 2.4151358981875311E-4 48-49 1.509459936367207E-5 4.528379809101621E-5 0.0 5.1783381631019765 2.4151358981875311E-4 50-51 1.509459936367207E-5 4.528379809101621E-5 0.0 5.985174688288976 2.4151358981875311E-4 52-53 1.509459936367207E-5 4.528379809101621E-5 0.0 6.756848344258302 2.4906088950058913E-4 54-55 1.509459936367207E-5 7.547299681836034E-5 0.0 7.591458932274458 2.5660818918242517E-4 56-57 1.509459936367207E-5 9.056759618203241E-5 0.0 8.505067106060391 2.5660818918242517E-4 58-59 1.509459936367207E-5 9.056759618203241E-5 0.0 9.4734913174355 2.641554888642612E-4 60-61 1.509459936367207E-5 9.056759618203241E-5 0.0 10.429039835553397 2.7170278854609726E-4 62-63 1.509459936367207E-5 9.056759618203241E-5 0.0 11.3874110437523 2.7170278854609726E-4 64-65 1.509459936367207E-5 9.056759618203241E-5 0.0 12.30372115082433 2.7170278854609726E-4 66-67 1.509459936367207E-5 9.056759618203241E-5 7.547299681836035E-6 13.304568561632607 2.7170278854609726E-4 68-69 1.509459936367207E-5 9.056759618203241E-5 1.509459936367207E-5 14.391424999615086 2.7170278854609726E-4 70-71 1.509459936367207E-5 1.1320949522754053E-4 1.509459936367207E-5 15.491225056551915 2.7170278854609726E-4 72-73 1.509459936367207E-5 1.3585139427304863E-4 1.509459936367207E-5 16.510570898880374 3.6227038472812966E-4 74-75 1.509459936367207E-5 1.5094599363672069E-4 1.509459936367207E-5 17.49036134357633 3.6227038472812966E-4 76-77 1.509459936367207E-5 1.5094599363672069E-4 1.509459936367207E-5 18.53234908495029 3.6227038472812966E-4 78-79 1.509459936367207E-5 1.5094599363672069E-4 1.509459936367207E-5 19.631839702600168 3.6227038472812966E-4 80-81 3.018919872734414E-5 1.5094599363672069E-4 1.509459936367207E-5 20.693918355727178 3.9245958345547377E-4 82-83 3.018919872734414E-5 1.5849329331855673E-4 1.509459936367207E-5 21.734894758943774 4.3774338154649E-4 84-85 3.018919872734414E-5 1.6604059300039277E-4 1.509459936367207E-5 22.739213927605697 4.3774338154649E-4 86-87 3.018919872734414E-5 1.735878926822288E-4 1.509459936367207E-5 23.745616150979806 4.3774338154649E-4 88 3.018919872734414E-5 1.8113519236406483E-4 1.509459936367207E-5 24.51420296137926 4.3774338154649E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12065 0.0 55.258488 1 TACCTGG 5785 0.0 45.554935 2 GTATCAA 22255 0.0 41.706955 1 ACCTGGG 6865 0.0 37.796688 3 ATCAACG 26715 0.0 34.18793 3 CAACGCA 26845 0.0 33.899292 5 TATCAAC 27595 0.0 33.323063 2 TCAACGC 28415 0.0 32.207996 4 AACGCAG 28770 0.0 31.974436 6 GTACATG 25665 0.0 29.134363 1 GTGGTAT 4500 0.0 28.688507 1 ACGCAGA 31760 0.0 28.476484 7 TACATGG 26130 0.0 28.167486 2 CGCAGAG 32460 0.0 27.75976 8 ACATGGG 26170 0.0 27.068535 3 CCTGGGG 6695 0.0 26.890171 4 CATGGGG 19020 0.0 26.814184 4 GTACTAG 2600 0.0 26.457533 1 ATGGGGG 15325 0.0 25.64215 5 GTACCTG 10555 0.0 25.53334 1 >>END_MODULE