FastQCFastQC Report
Mon 6 Jun 2016
ERR1211386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6624886
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267210.40334278959668135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225390.34021717505780474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134350.20279594245093424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125500.055.971021
TACCTGG57150.046.130682
GTATCAA225300.040.5061261
ACCTGGG67400.038.139983
CAACGCA274700.032.705445
ATCAACG274300.032.6721653
TATCAAC283550.032.0365262
TCAACGC288550.031.1403244
AACGCAG294250.030.85076
CCTGGGG61200.030.7160174
GTGGTAT47300.028.428981
GTACATG258100.027.616431
ACGCAGA326000.027.4280877
TGGGGGG139750.027.1328936
TACATGG259000.027.0533522
CGCAGAG331550.026.841418
ACATGGG256700.026.1613043
GTACTAG26750.025.4859351
CTGGGGG46300.025.4731985
CATGGGG183600.025.0079574
Produced by FastQC (version 0.11.5)