##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6624886 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08050795138211 33.0 31.0 34.0 30.0 34.0 2 32.209137334589606 33.0 31.0 34.0 30.0 34.0 3 32.28422421155624 34.0 31.0 34.0 30.0 34.0 4 35.928287973559094 37.0 35.0 37.0 35.0 37.0 5 35.787917407182555 37.0 35.0 37.0 35.0 37.0 6 35.82799854971089 37.0 35.0 37.0 35.0 37.0 7 35.7608502244416 37.0 35.0 37.0 33.0 37.0 8 35.74645676921837 37.0 35.0 37.0 33.0 37.0 9 37.33249326856341 39.0 37.0 39.0 34.0 39.0 10-11 37.3116707970522 39.0 37.0 39.0 34.0 39.0 12-13 37.22562509000154 39.0 37.0 39.0 33.0 39.0 14-15 38.422348399655476 40.0 38.0 41.0 33.5 41.0 16-17 38.40952266952216 40.0 38.0 41.0 34.0 41.0 18-19 38.42588159252853 40.0 38.0 41.0 34.0 41.0 20-21 38.389706177585545 40.0 38.0 41.0 34.0 41.0 22-23 38.28652968519006 40.0 38.0 41.0 34.0 41.0 24-25 38.16315375087209 40.0 37.5 41.0 33.0 41.0 26-27 38.02804795433461 40.0 37.0 41.0 33.0 41.0 28-29 37.94862915376959 40.0 37.0 41.0 33.0 41.0 30-31 37.98177847890514 40.0 37.0 41.0 33.0 41.0 32-33 37.80468048808689 40.0 37.0 41.0 33.0 41.0 34-35 37.68737862055287 40.0 37.0 41.0 33.0 41.0 36-37 37.54370799739044 40.0 36.0 41.0 32.5 41.0 38-39 37.42419280875173 40.0 36.0 41.0 32.0 41.0 40-41 37.34421807409214 40.0 36.0 41.0 32.0 41.0 42-43 37.20294454878167 40.0 36.0 41.0 31.0 41.0 44-45 37.03930943113587 39.0 36.0 41.0 31.0 41.0 46-47 36.80023044924849 39.0 35.0 41.0 30.5 41.0 48-49 36.56178687452132 39.0 35.0 41.0 30.0 41.0 50-51 36.371704584803425 39.0 35.0 41.0 29.5 41.0 52-53 36.16583470266507 39.0 35.0 40.0 29.0 41.0 54-55 35.96383417012761 38.5 35.0 40.0 28.5 41.0 56-57 35.728594575061365 38.0 35.0 40.0 28.0 41.0 58-59 35.46014452173215 38.0 34.0 40.0 27.5 41.0 60-61 35.19934350568448 37.5 34.0 40.0 27.0 41.0 62-63 34.89242071486211 37.0 34.0 40.0 26.5 41.0 64-65 34.5413866744273 36.5 33.5 39.5 26.0 41.0 66-67 34.18809961409147 36.0 33.0 39.0 26.0 40.5 68-69 33.82187761419593 35.5 33.0 39.0 25.5 40.0 70-71 33.453607428112726 35.0 32.5 38.0 25.0 40.0 72-73 33.10223904230201 35.0 32.0 37.5 25.0 40.0 74-75 32.74294984094821 35.0 32.0 37.0 24.0 39.0 76-77 31.72760104853125 34.0 30.5 36.0 23.0 38.0 78-79 32.20875204494085 35.0 31.5 36.0 24.0 38.0 80-81 32.11369991876087 35.0 32.0 35.5 24.0 37.0 82-83 31.85860828397651 34.5 31.5 35.0 24.0 37.0 84-85 31.599697111769167 34.0 31.0 35.0 23.0 36.5 86-87 31.357138598309465 34.0 31.0 35.0 23.0 36.0 88-89 31.144009723337128 34.0 31.0 35.0 21.5 36.0 90-91 30.935748101929605 34.0 31.0 35.0 20.0 35.0 92-93 30.764680479030133 34.0 30.5 35.0 20.0 35.0 94-95 30.615199114369666 34.0 30.0 35.0 20.0 35.0 96-97 30.45915529716285 34.0 30.0 35.0 19.0 35.0 98-99 30.269749622861436 34.0 29.5 35.0 19.0 35.0 100 28.432892430148986 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24579378898107151 1101 2 -0.24161701972381167 1101 3 -0.2457508819394647 1101 4 -0.11918362868589583 1101 5 -0.17087956282468753 1101 6 -0.251854878496637 1101 7 -0.19344396365428196 1101 8 -0.24400748981201303 1101 9 -0.3501392943987369 1101 10-11 -0.3840131646080067 1101 12-13 -0.4060633513238443 1101 14-15 -0.5083116602213238 1101 16-17 -0.5289877668926763 1101 18-19 -0.4859914602485418 1101 20-21 -0.5681154847419734 1101 22-23 -0.6192833683679169 1101 24-25 -0.6560084996538436 1101 26-27 -0.5968841840664965 1101 28-29 -0.5853627950416325 1101 30-31 -0.5499714546202057 1101 32-33 -0.4200350740574237 1101 34-35 -0.5584029094576053 1101 36-37 -0.534958315100539 1101 38-39 -0.6123751829387913 1101 40-41 -0.5927770270536428 1101 42-43 -0.5531972459494199 1101 44-45 -0.5280170408806129 1101 46-47 -0.6229262323341018 1101 48-49 -0.44998247570285343 1101 50-51 -0.4434923234240955 1101 52-53 -0.5972320427926405 1101 54-55 -0.6954176426848591 1101 56-57 -0.628067496356806 1101 58-59 -0.6003465022760182 1101 60-61 -0.6117271333921721 1101 62-63 -0.7972786784676273 1101 64-65 -0.7019438961301603 1101 66-67 -0.6599936636432062 1101 68-69 -0.6643223210357903 1101 70-71 -0.6892471009266856 1101 72-73 -0.6991473569580577 1101 74-75 -0.7028147118223629 1101 76-77 -0.5748756498816014 1101 78-79 -0.49160498156248167 1101 80-81 -0.4239322255544842 1101 82-83 -0.4695768815160939 1101 84-85 -0.5451760096529661 1101 86-87 -0.6513932684799819 1101 88-89 -0.6178297280364689 1101 90-91 -0.6179193233550713 1101 92-93 -0.8062973843948207 1101 94-95 -0.7824304371090101 1101 96-97 -0.7179631212376201 1101 98-99 -0.7696380521526258 1101 100 -0.7654161832784467 1102 1 -0.29129034714123136 1102 2 -0.24651141711697022 1102 3 -0.29374098526973924 1102 4 -0.15349332404795746 1102 5 -0.1962846072643032 1102 6 -0.2858308461248811 1102 7 -0.2724309970206491 1102 8 -0.31457116892813275 1102 9 -0.4135653957609904 1102 10-11 -0.4131010284678496 1102 12-13 -0.474834782772362 1102 14-15 -0.6111214434733085 1102 16-17 -0.6065457057813646 1102 18-19 -0.6157860153577275 1102 20-21 -0.5919265306938257 1102 22-23 -0.6012151721958503 1102 24-25 -0.7165304244342039 1102 26-27 -0.798563886265427 1102 28-29 -0.7328666992972757 1102 30-31 -0.64036811197672 1102 32-33 -0.6224159909480065 1102 34-35 -0.6877389684825346 1102 36-37 -0.6541674350265438 1102 38-39 -0.6760336791555517 1102 40-41 -0.6134089091875197 1102 42-43 -0.6835891433381676 1102 44-45 -0.7552573062993275 1102 46-47 -0.7549245774420328 1102 48-49 -0.8015247836567525 1102 50-51 -0.8216002423056779 1102 52-53 -0.8637321326700231 1102 54-55 -0.9235282501587747 1102 56-57 -0.9492852155825204 1102 58-59 -0.9516564705826553 1102 60-61 -0.9062614846585788 1102 62-63 -0.9143749071068399 1102 64-65 -0.9479317379512651 1102 66-67 -0.9554634462945373 1102 68-69 -0.9055345714328453 1102 70-71 -0.9787863015965854 1102 72-73 -1.0660789852269872 1102 74-75 -1.0020604761024856 1102 76-77 -0.792241604507435 1102 78-79 -0.73430252740215 1102 80-81 -0.6877824509766661 1102 82-83 -0.664053806216959 1102 84-85 -0.72289326005885 1102 86-87 -0.7616159203617094 1102 88-89 -0.7786338280348808 1102 90-91 -0.7103655216965628 1102 92-93 -0.9076848360553633 1102 94-95 -0.886279883005038 1102 96-97 -0.9992355629785301 1102 98-99 -1.0956868460732956 1102 100 -0.9754460901416344 1103 1 -0.3533879584624984 1103 2 -0.3827970254871502 1103 3 -0.37603669359782543 1103 4 -0.2322660181163272 1103 5 -0.2340809103397632 1103 6 -0.32849557891184844 1103 7 -0.3179538902475443 1103 8 -0.38591588662237086 1103 9 -0.5211908606708917 1103 10-11 -0.531782312989904 1103 12-13 -0.5991932013164458 1103 14-15 -0.7534594372318466 1103 16-17 -0.7945160514160605 1103 18-19 -0.734520375750499 1103 20-21 -0.722857180537595 1103 22-23 -0.817472394069199 1103 24-25 -0.7413056952451527 1103 26-27 -0.8245992543060794 1103 28-29 -0.8775356497144884 1103 30-31 -0.6505663677151148 1103 32-33 -0.7185887246331291 1103 34-35 -0.6594086890785817 1103 36-37 -0.6150668048291195 1103 38-39 -0.8141933948672957 1103 40-41 -0.8276167576041544 1103 42-43 -0.7666819817993513 1103 44-45 -0.7448579905646966 1103 46-47 -0.7245902292186912 1103 48-49 -0.8309047582795515 1103 50-51 -0.8487086192653877 1103 52-53 -0.8792257399211465 1103 54-55 -0.9351211942756663 1103 56-57 -0.9998595758694719 1103 58-59 -1.063829760489675 1103 60-61 -1.0127736790465534 1103 62-63 -1.0297587911560768 1103 64-65 -0.9412477331668327 1103 66-67 -0.9988032843155779 1103 68-69 -0.9198468685333765 1103 70-71 -0.8576862620128978 1103 72-73 -0.9076869460123547 1103 74-75 -0.9288653781303822 1103 76-77 -0.8716946948624091 1103 78-79 -0.8092152333026341 1103 80-81 -0.6781233038877232 1103 82-83 -0.5755367295102545 1103 84-85 -0.6248723782586474 1103 86-87 -0.6971363433284488 1103 88-89 -0.7376759557258339 1103 90-91 -0.8947358847917322 1103 92-93 -0.8480367340385797 1103 94-95 -0.9750207546993259 1103 96-97 -1.0310219744312796 1103 98-99 -1.0746072927836288 1103 100 -1.0938284301955967 1104 1 -0.3695002381406667 1104 2 -0.3394123929552286 1104 3 -0.34038367732465247 1104 4 -0.1502393120586092 1104 5 -0.2468555704012445 1104 6 -0.3028943827680166 1104 7 -0.32462891242644076 1104 8 -0.33766212519031313 1104 9 -0.42774068582536984 1104 10-11 -0.4451640093173381 1104 12-13 -0.46855079548024037 1104 14-15 -0.6350575203803146 1104 16-17 -0.6846655791624912 1104 18-19 -0.6736249408240482 1104 20-21 -0.5979753734852338 1104 22-23 -0.6444245020290538 1104 24-25 -0.7131686787360465 1104 26-27 -0.7717903739778222 1104 28-29 -0.8216512159230831 1104 30-31 -0.7321453853406439 1104 32-33 -0.6494227136066542 1104 34-35 -0.8210590503413471 1104 36-37 -0.7005640628069969 1104 38-39 -0.8336750655117555 1104 40-41 -0.8539388730735595 1104 42-43 -0.7798770085602698 1104 44-45 -0.7705014716345318 1104 46-47 -0.8250600680606226 1104 48-49 -0.8961679805195146 1104 50-51 -0.8776355784249077 1104 52-53 -0.836898434027951 1104 54-55 -0.7905422086114129 1104 56-57 -0.8750765150399005 1104 58-59 -1.0022342434677967 1104 60-61 -1.001612384939527 1104 62-63 -1.0071120863388288 1104 64-65 -1.0392977401315733 1104 66-67 -0.9250546970011015 1104 68-69 -0.9279640956428565 1104 70-71 -0.9908898835886149 1104 72-73 -0.963462513415827 1104 74-75 -0.9429979852862473 1104 76-77 -0.832389126487076 1104 78-79 -0.7358270513873215 1104 80-81 -0.8179803371556069 1104 82-83 -0.843464493562049 1104 84-85 -0.7139921669908382 1104 86-87 -0.6669300786260592 1104 88-89 -0.8171973918364195 1104 90-91 -0.7857408785099054 1104 92-93 -0.9289699196229293 1104 94-95 -0.9325971308803531 1104 96-97 -1.0260349442504513 1104 98-99 -1.1573861197485051 1104 100 -1.1221895825877546 1105 1 -0.38872802306527277 1105 2 -0.36877057464526786 1105 3 -0.348597205720214 1105 4 -0.23524553842459994 1105 5 -0.21379668954875797 1105 6 -0.2606192998209238 1105 7 -0.349019613482632 1105 8 -0.38364551096634614 1105 9 -0.42770428925395976 1105 10-11 -0.5555982656108256 1105 12-13 -0.4902304703985081 1105 14-15 -0.7099513222834872 1105 16-17 -0.6430008366754052 1105 18-19 -0.600586008164008 1105 20-21 -0.7014388355946366 1105 22-23 -0.8081126583321492 1105 24-25 -0.7266819493805983 1105 26-27 -0.6938338231891663 1105 28-29 -0.716150502919831 1105 30-31 -0.6475841063954277 1105 32-33 -0.5734283607380917 1105 34-35 -0.7011527790898029 1105 36-37 -0.8752337479697516 1105 38-39 -0.9298082740917835 1105 40-41 -0.8663866865980552 1105 42-43 -0.8023137800190199 1105 44-45 -0.7722468720078552 1105 46-47 -0.8393107614232775 1105 48-49 -0.7568813724611587 1105 50-51 -0.7085363233795121 1105 52-53 -0.7134563249282593 1105 54-55 -0.8171627912101869 1105 56-57 -0.8812616989341748 1105 58-59 -0.8533018793307647 1105 60-61 -0.8669684001695188 1105 62-63 -0.7727259695491213 1105 64-65 -0.8119242376916276 1105 66-67 -0.8494690146200199 1105 68-69 -0.8763000930510074 1105 70-71 -0.9831172349181259 1105 72-73 -0.9689230226971759 1105 74-75 -0.8850370966856218 1105 76-77 -0.8080657152206818 1105 78-79 -0.7547573096277738 1105 80-81 -0.6196839997806052 1105 82-83 -0.7076903806274508 1105 84-85 -0.6404858747042503 1105 86-87 -0.6222526890349549 1105 88-89 -0.6438171795064243 1105 90-91 -0.7423711201578804 1105 92-93 -0.761507340495946 1105 94-95 -0.7982451561483472 1105 96-97 -0.8338065720090739 1105 98-99 -0.8213805496108328 1105 100 -0.8321466077813895 1106 1 -0.38301118245057353 1106 2 -0.3665998349824129 1106 3 -0.3801042759844151 1106 4 -0.2517707186143383 1106 5 -0.31935147221013693 1106 6 -0.3773648875710336 1106 7 -0.3921565551856432 1106 8 -0.417049216705081 1106 9 -0.5374420688991748 1106 10-11 -0.5075570370931786 1106 12-13 -0.5760069300403856 1106 14-15 -0.7004145107389732 1106 16-17 -0.8183352383202518 1106 18-19 -0.7122304162590112 1106 20-21 -0.6921440414194819 1106 22-23 -0.6739095379944047 1106 24-25 -0.8322720197358322 1106 26-27 -0.7481161331715711 1106 28-29 -0.8387798451993689 1106 30-31 -0.7722388998461014 1106 32-33 -0.8326124905193524 1106 34-35 -0.7259886405677989 1106 36-37 -0.8168390163912136 1106 38-39 -0.8864138961708932 1106 40-41 -0.8364183003120971 1106 42-43 -0.8445375355383788 1106 44-45 -0.9048063821272194 1106 46-47 -0.8730130072976223 1106 48-49 -0.8761835614380047 1106 50-51 -0.9945622092450535 1106 52-53 -0.9831207046088508 1106 54-55 -0.9796640657048812 1106 56-57 -0.9561711883415427 1106 58-59 -0.9648061905969527 1106 60-61 -1.058036468101136 1106 62-63 -1.0148171541991573 1106 64-65 -1.1437838413858472 1106 66-67 -0.9995958629496045 1106 68-69 -0.9257520095865601 1106 70-71 -0.9608811474427412 1106 72-73 -0.9530460587067111 1106 74-75 -0.948841658135315 1106 76-77 -0.9685996227128975 1106 78-79 -0.862493917908921 1106 80-81 -0.8678060620996106 1106 82-83 -0.8476531630402526 1106 84-85 -0.7975734938919494 1106 86-87 -0.7742495118933839 1106 88-89 -0.9201820052328387 1106 90-91 -0.9350486426307576 1106 92-93 -0.8698651527151391 1106 94-95 -0.8280550210573736 1106 96-97 -0.8601070289222541 1106 98-99 -0.9176437389719396 1106 100 -1.1019315667610066 1107 1 -0.3711242629298894 1107 2 -0.33822918249869005 1107 3 -0.370300803925943 1107 4 -0.22791872610277863 1107 5 -0.2559493082472315 1107 6 -0.29714856273523793 1107 7 -0.30698822799343617 1107 8 -0.42849530103262623 1107 9 -0.5892336540877494 1107 10-11 -0.51097100396683 1107 12-13 -0.5167550378828523 1107 14-15 -0.612669748956705 1107 16-17 -0.6523366473652956 1107 18-19 -0.6915052076314154 1107 20-21 -0.6178253511188103 1107 22-23 -0.6455185407978092 1107 24-25 -0.6732348188825057 1107 26-27 -0.6752698542493292 1107 28-29 -0.6871041802150657 1107 30-31 -0.7192712165789459 1107 32-33 -0.7150664211575872 1107 34-35 -0.6785739474375205 1107 36-37 -0.6878816968200354 1107 38-39 -0.7118872233591276 1107 40-41 -0.6982769803788855 1107 42-43 -0.6895919293612138 1107 44-45 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1.0526998313118554 2311 38-39 1.056344764695723 2311 40-41 1.0334435389029082 2311 42-43 1.0982820035808132 2311 44-45 1.0684134351812347 2311 46-47 1.0861849060259203 2311 48-49 1.0347704143543552 2311 50-51 1.0952667973698382 2311 52-53 1.1487637346030652 2311 54-55 1.1419615952360616 2311 56-57 1.1894449806073268 2311 58-59 1.2218921568014807 2311 60-61 1.2187054675044138 2311 62-63 1.2295290954372788 2311 64-65 1.266535767471325 2311 66-67 1.3016741123190059 2311 68-69 1.2898217789797357 2311 70-71 1.3264215536533115 2311 72-73 1.3149426618535571 2311 74-75 1.1664375149202044 2311 76-77 1.0686628935553308 2311 78-79 1.043791768404624 2311 80-81 1.093085271784254 2311 82-83 1.0720155009304868 2311 84-85 1.1050523887598125 2311 86-87 1.1275517631697518 2311 88-89 1.1607015043189435 2311 90-91 1.199309132321023 2311 92-93 1.2442875913283196 2311 94-95 1.28755186869871 2311 96-97 1.2886417742890153 2311 98-99 1.3528250217076625 2311 100 1.4271550364546677 2312 1 0.16506280442398236 2312 2 0.16619514552649406 2312 3 0.31238687005485843 2312 4 0.20986320196524133 2312 5 0.3636718568290789 2312 6 0.4026917487667063 2312 7 0.4193227946594149 2312 8 0.26637964011672466 2312 9 0.35745133218968306 2312 10-11 0.2971001174898049 2312 12-13 0.41327403156189035 2312 14-15 0.7360626195198492 2312 16-17 0.7798811397001586 2312 18-19 0.8330638358504743 2312 20-21 0.8429092273995451 2312 22-23 0.4420800360776269 2312 24-25 0.3884418842274826 2312 26-27 0.7426220649445696 2312 28-29 0.8879955924467566 2312 30-31 0.8319005847047052 2312 32-33 0.6893183969424967 2312 34-35 0.8677037205292422 2312 36-37 0.9760700245899798 2312 38-39 0.9596843713513934 2312 40-41 0.9358962316878277 2312 42-43 0.8356755472405553 2312 44-45 0.7160827878675704 2312 46-47 0.9384040690859052 2312 48-49 1.041632350706898 2312 50-51 1.0308052229350224 2312 52-53 1.0981732353830438 2312 54-55 1.058389270889684 2312 56-57 1.0424492294008445 2312 58-59 0.9810721065515011 2312 60-61 1.0574934327890233 2312 62-63 1.095474585713859 2312 64-65 1.1293806983544954 2312 66-67 1.1082921588626107 2312 68-69 1.1326692506232234 2312 70-71 1.016478947973205 2312 72-73 1.1281721652591656 2312 74-75 1.1750134417735723 2312 76-77 1.0575206556019587 2312 78-79 0.9678454749573362 2312 80-81 0.8552027689774988 2312 82-83 0.8164798615767701 2312 84-85 0.7308274561659864 2312 86-87 0.8989691232273991 2312 88-89 0.5296092661286451 2312 90-91 0.9295089544439428 2312 92-93 1.108194831383873 2312 94-95 1.2100800963481078 2312 96-97 1.2730782111169319 2312 98-99 1.224833939493422 2312 100 1.3754325301625094 2313 1 -0.3231060777073509 2313 2 0.013905637779849656 2313 3 0.27091058624789355 2313 4 0.07339433653353922 2313 5 0.22167402597285957 2313 6 0.3348052250899016 2313 7 0.15929171043116241 2313 8 0.05690785295245604 2313 9 0.2839719383522308 2313 10-11 0.40854686243775973 2313 12-13 0.6166960698938837 2313 14-15 0.6145518331841302 2313 16-17 0.7978558036828289 2313 18-19 0.6043337636280697 2313 20-21 0.8076220067591962 2313 22-23 0.8414717252867945 2313 24-25 0.7313830333913884 2313 26-27 0.8988966258155386 2313 28-29 0.8027029157888492 2313 30-31 0.7850697672686806 2313 32-33 0.9406954636226672 2313 34-35 0.9176634585359054 2313 36-37 0.7793380886803973 2313 38-39 0.5762050619164256 2313 40-41 0.7596376751350533 2313 42-43 0.8267484273767707 2313 44-45 0.9709636125682408 2313 46-47 0.9855041706301293 2313 48-49 0.9932238599780092 2313 50-51 0.976639176727069 2313 52-53 1.0085036369358846 2313 54-55 1.0055265396695887 2313 56-57 1.0446769022817364 2313 58-59 0.8908583632954077 2313 60-61 0.8844730176373332 2313 62-63 0.9728166289813842 2313 64-65 1.0569058950072616 2313 66-67 1.079622544941671 2313 68-69 1.0288686671609284 2313 70-71 0.9529372932384419 2313 72-73 1.0538346292851273 2313 74-75 0.7523903211606182 2313 76-77 0.9045107123867417 2313 78-79 0.7297019135705156 2313 80-81 0.8293101976619255 2313 82-83 0.9020247408882547 2313 84-85 0.9194586629385988 2313 86-87 0.997155735908624 2313 88-89 0.9756967288031575 2313 90-91 1.0266144115265838 2313 92-93 0.7783853519000061 2313 94-95 0.7889869214342156 2313 96-97 1.0776403461138777 2313 98-99 1.1488239281533446 2313 100 1.246063521105924 2314 1 -0.34740589325178206 2314 2 -0.13072765082824134 2314 3 0.1541216891776358 2314 4 -0.16535588233566756 2314 5 0.19176241357518364 2314 6 0.2040744316195955 2314 7 0.12491785703191738 2314 8 0.17638997044932836 2314 9 0.28290925747881346 2314 10-11 0.4267305449719885 2314 12-13 0.533569977589984 2314 14-15 0.6131711552940615 2314 16-17 0.776681805594464 2314 18-19 0.7619173341858314 2314 20-21 0.47009522591343966 2314 22-23 0.6581780622975089 2314 24-25 0.8476688916633179 2314 26-27 0.8056939489898838 2314 28-29 0.741349576608755 2314 30-31 0.730660275908221 2314 32-33 0.8418328915736168 2314 34-35 0.9059328859048676 2314 36-37 0.7892476022401027 2314 38-39 0.7993312868392053 2314 40-41 0.8398932893824735 2314 42-43 0.5974254890574286 2314 44-45 0.7314646255650814 2314 46-47 0.8520331635204101 2314 48-49 0.7161605263890465 2314 50-51 0.8696846425162974 2314 52-53 0.9172936068736846 2314 54-55 0.991976013124571 2314 56-57 0.9854625230889908 2314 58-59 0.9698696664604469 2314 60-61 1.018428948574801 2314 62-63 0.7115923145200966 2314 64-65 0.8543524625711001 2314 66-67 1.0066306494930117 2314 68-69 1.006232353689093 2314 70-71 1.0500853337752218 2314 72-73 1.0122404945762469 2314 74-75 1.0681058605763951 2314 76-77 0.9511952499283645 2314 78-79 0.9076035138191472 2314 80-81 0.8586887398113774 2314 82-83 0.8036775085194101 2314 84-85 0.7365737159698149 2314 86-87 0.863065167974991 2314 88-89 0.9118435236834515 2314 90-91 1.067262051035403 2314 92-93 1.051344803772711 2314 94-95 1.0645830865574055 2314 96-97 1.1003128414765406 2314 98-99 1.0817365040560354 2314 100 1.2184678380235354 2315 1 -0.12261895634976838 2315 2 0.08576805035335155 2315 3 0.09983487210298847 2315 4 -0.07696186466814936 2315 5 0.23423075693255413 2315 6 0.32675952771668193 2315 7 0.39705807791958847 2315 8 0.3604399718344524 2315 9 0.5590920948904596 2315 10-11 0.5797096541071127 2315 12-13 0.6230453698456628 2315 14-15 0.7345577187511623 2315 16-17 0.76562278080889 2315 18-19 0.7988327620941007 2315 20-21 0.8091354654119982 2315 22-23 0.8684343738717217 2315 24-25 0.786624796493804 2315 26-27 0.9063473567444689 2315 28-29 0.9044785471104007 2315 30-31 0.8254742608867645 2315 32-33 0.888389077222179 2315 34-35 0.863159907729063 2315 36-37 0.8728706500719525 2315 38-39 0.8675534967905065 2315 40-41 0.8781043681243901 2315 42-43 0.962126819578117 2315 44-45 0.7994974264707508 2315 46-47 0.8287732679087298 2315 48-49 0.8556888509198544 2315 50-51 0.778075737474424 2315 52-53 0.886894129019808 2315 54-55 0.8695301534343329 2315 56-57 0.9953477695605955 2315 58-59 0.9980335133260851 2315 60-61 1.040666546628266 2315 62-63 1.0371657800372418 2315 64-65 1.0587175253359078 2315 66-67 1.1107491040044408 2315 68-69 1.0006930106182281 2315 70-71 0.6601361260767433 2315 72-73 0.8687782719896191 2315 74-75 1.01908256981757 2315 76-77 0.9792720848348786 2315 78-79 0.9272916893587038 2315 80-81 0.9064620238304926 2315 82-83 0.8223960956683065 2315 84-85 0.8094927589646908 2315 86-87 0.7647288254780769 2315 88-89 0.9129338225751091 2315 90-91 0.9829677282496654 2315 92-93 0.9833274809559249 2315 94-95 1.0278072403335656 2315 96-97 1.1108656444422351 2315 98-99 1.002734525649501 2315 100 0.9944785107468306 2316 1 0.48493198520102254 2316 2 0.4794135911420341 2316 3 0.4875193384831533 2316 4 0.29534215662597774 2316 5 0.4004696048268741 2316 6 0.4567360517938468 2316 7 0.5073427063159883 2316 8 0.5276405918911635 2316 9 0.4722951922799794 2316 10-11 0.5099457174852375 2316 12-13 0.6607573876934438 2316 14-15 0.8034804726152345 2316 16-17 0.82471874747268 2316 18-19 0.8552042390195425 2316 20-21 0.8816446619061082 2316 22-23 0.898888065333054 2316 24-25 0.8925709961141948 2316 26-27 0.9593647559545957 2316 28-29 0.9045011566657237 2316 30-31 0.8273598364871049 2316 32-33 0.8143819938554344 2316 34-35 0.6950657887027987 2316 36-37 0.6804460436652207 2316 38-39 0.6822211936558205 2316 40-41 0.755376191572303 2316 42-43 0.9197929181088114 2316 44-45 0.8804587492011677 2316 46-47 0.9203727466412204 2316 48-49 0.5687469375193288 2316 50-51 0.6540150533666065 2316 52-53 0.7828445108714348 2316 54-55 0.8678493514269547 2316 56-57 0.9086599446421175 2316 58-59 0.8372434303093215 2316 60-61 0.8605249375416193 2316 62-63 0.9688840952056736 2316 64-65 0.9485210205555035 2316 66-67 0.9244919550929751 2316 68-69 0.9128912627263901 2316 70-71 0.8243603782844886 2316 72-73 0.846995015433734 2316 74-75 0.926932751515622 2316 76-77 0.7529557856478313 2316 78-79 0.7467224147848341 2316 80-81 0.6792476117917516 2316 82-83 0.5899311623988375 2316 84-85 0.7282547412625924 2316 86-87 0.728844931967334 2316 88-89 0.6564504137887077 2316 90-91 0.7397501043358155 2316 92-93 0.7604065085102114 2316 94-95 0.6545402359245962 2316 96-97 0.7438888175962965 2316 98-99 0.8358873197674725 2316 100 0.5414435695874715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 14.0 12 35.0 13 213.0 14 971.0 15 2764.0 16 6610.0 17 13224.0 18 20531.0 19 28776.0 20 37879.0 21 49560.0 22 58248.0 23 59801.0 24 59488.0 25 63315.0 26 72520.0 27 87241.0 28 106739.0 29 128768.0 30 157253.0 31 194991.0 32 244880.0 33 319040.0 34 448399.0 35 615394.0 36 928286.0 37 1439794.0 38 1290648.0 39 189475.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.057740804160076 19.92225834405863 13.296351325350553 23.723649526430744 2 15.94813857929027 21.202900698970517 39.877893747907514 22.9710669738317 3 18.183769501845013 27.210973894494185 30.978827409256553 23.62642919440425 4 12.615282839814334 17.509978221375057 38.26821132965556 31.606527609155055 5 12.550563407346827 38.122722221995794 34.54872691993769 14.777987450719687 6 29.14128635571993 37.57880512962789 18.9780624149608 14.301846099691376 7 26.26457179317152 33.507082628476844 22.96055274132885 17.26779283702279 8 25.85431577981311 35.187957849090616 20.830483588868177 18.1272427822281 9 27.356972240973516 15.612580622177996 20.76859608424678 36.261851052601706 10-11 24.687645192229606 26.1308804834171 28.916548600886543 20.26492572346675 12-13 25.031611955379635 24.427245105448907 28.436797297213666 22.104345641957792 14-15 22.906217581972207 25.803242548075385 26.504424795458636 24.786115074493775 16-17 21.758209694634516 28.2098274670896 27.424869971249294 22.607092867026594 18-19 21.33768037668875 27.667834586134767 29.536953541540186 21.457531495636303 20-21 22.40300020302239 27.06294524357781 29.05790817500983 21.476146378389966 22-23 22.35601488085984 26.748490464590635 28.915040953157533 21.980453701391994 24-25 21.841963095801088 26.626168341388066 29.370896059598333 22.16097250321251 26-27 21.827707197936267 26.807476658085385 29.125433875455954 22.239382268522398 28-29 21.527650782569317 27.663303678405992 29.072649225921488 21.7363963131032 30-31 22.60123097591257 26.843033589566517 29.19800900401143 21.357726430509487 32-33 22.42457464387372 27.02491919662129 28.73757567143288 21.81293048807211 34-35 22.368537169018285 27.09032014108278 29.17398767737543 21.367155012523504 36-37 22.11399896600125 26.82922502035383 29.046192021781618 22.0105839918633 38-39 22.020839051586723 26.961183514641597 29.198647366976445 21.81933006679524 40-41 21.92187942579139 26.34245373251976 29.706306134553945 22.029360707134906 42-43 21.710586744811657 26.752611162379065 28.998633086580465 22.538169006228813 44-45 21.050851433780007 27.110574831399475 29.503280226344526 22.335293508475996 46-47 21.10426587792833 26.53712036029464 29.13509418603387 23.223519575743158 48-49 21.41056521921917 26.67286386347167 29.091416024498752 22.82515489281041 50-51 21.286479576509475 26.749754611359037 28.94636412685272 23.017401685278767 52-53 20.933669594991404 27.141395963954277 28.633867819276215 23.2910666217781 54-55 20.779533288447546 26.751013704254987 28.957567834401637 23.511885172895827 56-57 21.253516677969095 26.57859452132234 28.422524400225974 23.74536440048259 58-59 20.705413671399718 26.752619883641238 28.758351775422152 23.783614669536888 60-61 21.272328398147227 26.963162751703813 28.247387402033475 23.51712144811549 62-63 21.200571427536357 26.704335156982566 28.140323076337864 23.95477033914321 64-65 20.70111323954277 26.40676784903327 28.14664246615713 24.745476445266828 66-67 20.806933416976435 26.266540950322238 27.929838560913822 24.99668707178751 68-69 20.392550610858397 26.387188840098148 28.102899601707172 25.11736094733628 70-71 20.45693175585997 26.104734632550752 28.158172913305034 25.28016069828424 72-73 20.327619885790448 26.328215917985847 27.77287292425974 25.571291271963965 74-75 20.312397255873538 26.301990556920636 27.7120504766346 25.673561710571224 76-77 20.047728282480165 26.151512760822946 27.833183537592816 25.967575419104072 78-79 19.76117497221679 26.161182701621794 27.94821509998144 26.12942722617998 80-81 19.60525361431466 26.231005936917168 27.73222081579269 26.43151963297548 82-83 19.952849421862613 25.73866231901334 28.068643438700665 26.23984482042338 84-85 19.957755411337295 25.987905026770786 27.549276984461564 26.505062577430355 86-87 19.42409814429963 26.17791448195956 27.830370830480927 26.56761654325988 88-89 19.486166915324972 25.498341153559068 27.901916456246056 27.113575474869904 90-91 19.89649287078566 25.072121130581486 27.88350510280121 27.147880895831644 92-93 19.678176674206934 25.12506216871661 27.930958677091986 27.26580247998447 94-95 19.10003042549117 24.849965192966376 28.308175882737395 27.74182849880506 96-97 19.11874711918156 24.721250743342672 28.36572995363595 27.794272183839823 98-99 19.005884285482207 24.965335045242597 28.120638571948803 27.908142097326387 100 18.89661746593325 24.990798074887817 28.226071144846305 27.88651331433263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2983.0 1 2079.5 2 2211.5 3 3141.5 4 2342.0 5 1749.5 6 2386.5 7 3946.5 8 5838.5 9 6795.5 10 6013.5 11 5144.5 12 5384.5 13 5935.5 14 6604.0 15 7344.5 16 8297.0 17 9196.0 18 9894.0 19 10767.5 20 11776.5 21 12957.5 22 14554.0 23 16856.5 24 19697.5 25 23241.0 26 28344.5 27 34552.0 28 41492.0 29 51196.0 30 64495.0 31 76888.0 32 91557.5 33 108547.5 34 124268.0 35 139221.5 36 154909.0 37 174466.0 38 185586.5 39 187206.0 40 187626.0 41 189928.0 42 197148.0 43 208600.0 44 222445.0 45 240699.5 46 256684.0 47 270856.0 48 286528.5 49 296556.5 50 302498.0 51 303347.0 52 292975.0 53 273053.0 54 250665.0 55 222487.5 56 193854.5 57 164305.0 58 135266.5 59 112556.5 60 88188.0 61 66215.5 62 51347.0 63 39532.0 64 28466.0 65 19375.5 66 13803.5 67 10498.5 68 8010.0 69 5981.0 70 4517.0 71 3406.0 72 2551.0 73 1976.5 74 1502.5 75 1055.0 76 732.0 77 458.0 78 272.5 79 183.5 80 129.5 81 85.0 82 53.5 83 29.0 84 18.5 85 15.0 86 11.0 87 6.5 88 6.0 89 3.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05234807059321474 2 0.0 3 0.0 4 0.014490815389125186 5 0.0042717716199191956 6 0.0 7 2.2641899045508103E-4 8 3.4717578536445757E-4 9 0.002384946699460187 10-11 7.245407694562593E-4 12-13 2.867973879097693E-4 14-15 5.283109777285224E-5 16-17 7.547299681836034E-5 18-19 0.0 20-21 1.5094599363672068E-5 22-23 0.0 24-25 4.528379809101621E-5 26-27 1.5094599363672068E-5 28-29 0.001109453053229897 30-31 9.207705611839962E-4 32-33 0.014136092304078892 34-35 0.023253230319736824 36-37 0.031004307092982433 38-39 0.020528655134594016 40-41 1.7358789268222879E-4 42-43 0.0016075748322310756 44-45 0.0097813003876595 46-47 0.014702139780216596 48-49 0.005818968054695583 50-51 0.010566219554570449 52-53 0.016241788915311146 54-55 0.010053003176205598 56-57 0.003071750970507266 58-59 0.00824165125256495 60-61 0.0016679532296857637 62-63 0.0011170003529117332 64-65 0.02013619555113854 66-67 0.04680080532706525 68-69 0.056642484112179446 70-71 0.04957821160998091 72-73 0.04166864154341675 74-75 0.029291070065205652 76-77 0.02986466484102519 78-79 0.012551159370893326 80-81 0.0022868318035963184 82-83 0.02791746152311149 84-85 0.048446116657705506 86-87 0.049646137307117436 88-89 0.060680289441961716 90-91 0.03542702470653834 92-93 0.02686838686733628 94-95 0.007645414577699903 96-97 0.0037661025412361816 98-99 0.022370196256962006 100 0.01920033039059087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6624886.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.197674391647894 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35669531303347 45.96495482076256 2 14.41478707638089 17.354733176978215 3 4.399054414776776 7.9443853587562065 4 1.7393039778039694 4.188082180957654 5 0.888722410921988 2.6749511158621067 6 0.5441793804508299 1.9654999893016643 7 0.3461594962191843 1.4586597648685526 8 0.24488061397582883 1.1792994771955012 9 0.17165866181722808 0.9300107007521546 >10 0.821511791826113 8.524091298773113 >50 0.0441149329267875 1.8351255266704989 >100 0.026168281024241482 2.9930420485092593 >500 0.0018324254088919979 0.7392593900441361 >1k 8.110655714117078E-4 1.0022355005153205 >5k 3.003946560451109E-5 0.11195222256304449 >10k+ 9.011839681353327E-5 1.1337174274899167 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26721 0.40334278959668135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22539 0.34021717505780474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13435 0.20279594245093424 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.509459936367207E-5 1.509459936367207E-5 2 0.0 0.0 0.0 1.509459936367207E-5 1.509459936367207E-5 3 0.0 0.0 0.0 1.509459936367207E-5 1.509459936367207E-5 4 0.0 0.0 0.0 1.509459936367207E-5 1.509459936367207E-5 5 3.018919872734414E-5 3.018919872734414E-5 0.0 1.509459936367207E-5 1.509459936367207E-5 6 3.018919872734414E-5 3.018919872734414E-5 0.0 1.509459936367207E-5 3.018919872734414E-5 7 3.018919872734414E-5 3.018919872734414E-5 0.0 1.509459936367207E-5 3.018919872734414E-5 8 3.018919872734414E-5 3.018919872734414E-5 0.0 1.509459936367207E-5 3.018919872734414E-5 9 3.018919872734414E-5 3.018919872734414E-5 0.0 6.037839745468828E-5 3.018919872734414E-5 10-11 3.018919872734414E-5 3.018919872734414E-5 0.0 9.056759618203241E-5 3.018919872734414E-5 12-13 3.018919872734414E-5 3.018919872734414E-5 0.0 1.3585139427304863E-4 6.037839745468828E-5 14-15 3.018919872734414E-5 3.018919872734414E-5 0.0 1.9622979172773689E-4 6.037839745468828E-5 16-17 3.018919872734414E-5 3.018919872734414E-5 0.0 2.415135898187531E-4 6.037839745468828E-5 18-19 3.018919872734414E-5 4.528379809101621E-5 0.0 3.698176844099657E-4 6.037839745468828E-5 20-21 3.018919872734414E-5 4.528379809101621E-5 0.0 9.283178608658323E-4 6.792569713652431E-5 22-23 3.018919872734414E-5 4.528379809101621E-5 0.0 0.003018919872734414 7.547299681836034E-5 24-25 3.018919872734414E-5 4.528379809101621E-5 0.0 0.008596374337611245 9.056759618203241E-5 26-27 3.018919872734414E-5 4.528379809101621E-5 0.0 0.018068235438315466 1.0566219554570449E-4 28-29 3.018919872734414E-5 4.528379809101621E-5 0.0 0.04850649505516019 1.0566219554570449E-4 30-31 3.018919872734414E-5 6.037839745468828E-5 0.0 0.14271188968383758 1.0566219554570449E-4 32-33 3.018919872734414E-5 6.037839745468828E-5 0.0 0.3347831192868828 1.0566219554570449E-4 34-35 3.018919872734414E-5 6.037839745468828E-5 0.0 0.6271428670621653 1.3585139427304863E-4 36-37 3.018919872734414E-5 6.037839745468828E-5 0.0 1.0597918213234159 1.3585139427304863E-4 38-39 3.018919872734414E-5 6.037839745468828E-5 0.0 1.6610625450762475 1.3585139427304863E-4 40-41 3.018919872734414E-5 6.037839745468828E-5 0.0 2.288733723116141 1.3585139427304863E-4 42-43 3.773649840918017E-5 6.037839745468828E-5 0.0 2.8799137071943575 1.4339869395488464E-4 44-45 4.528379809101621E-5 6.037839745468828E-5 0.0 3.587405126669349 1.6604059300039277E-4 46-47 4.528379809101621E-5 6.037839745468828E-5 0.0 4.386611331878012 1.8113519236406483E-4 48-49 4.528379809101621E-5 6.037839745468828E-5 0.0 5.192421424308282 1.8113519236406483E-4 50-51 4.528379809101621E-5 6.037839745468828E-5 0.0 5.999959848365693 1.8113519236406483E-4 52-53 4.528379809101621E-5 6.037839745468828E-5 0.0 6.772131626114019 2.1132439109140897E-4 54-55 4.528379809101621E-5 9.811489586386844E-5 0.0 7.607391281902814 2.4151358981875311E-4 56-57 4.528379809101621E-5 1.2075679490937656E-4 0.0 8.52154286126584 2.5660818918242517E-4 58-59 4.528379809101621E-5 1.3585139427304863E-4 0.0 9.490661424211677 2.5660818918242517E-4 60-61 4.528379809101621E-5 1.3585139427304863E-4 0.0 10.445357097465527 2.5660818918242517E-4 62-63 4.528379809101621E-5 1.3585139427304863E-4 0.0 11.402966028396564 2.7170278854609726E-4 64-65 4.528379809101621E-5 1.3585139427304863E-4 0.0 12.317811959330319 2.7170278854609726E-4 66-67 4.528379809101621E-5 1.3585139427304863E-4 0.0 13.317142362902548 2.7170278854609726E-4 68-69 4.528379809101621E-5 1.5094599363672069E-4 0.0 14.40273085453848 2.867973879097693E-4 70-71 4.528379809101621E-5 1.5094599363672069E-4 0.0 15.501655424712215 3.3208118600078554E-4 72-73 4.528379809101621E-5 1.5094599363672069E-4 0.0 16.52133334822667 3.3208118600078554E-4 74-75 4.528379809101621E-5 1.5094599363672069E-4 0.0 17.50213513107999 3.3208118600078554E-4 76-77 4.528379809101621E-5 1.5094599363672069E-4 0.0 18.54603988657314 3.3208118600078554E-4 78-79 4.528379809101621E-5 1.5094599363672069E-4 0.0 19.645741828614106 3.3208118600078554E-4 80-81 4.528379809101621E-5 1.6604059300039277E-4 0.0 20.70925446868067 3.471757853644576E-4 82-83 4.528379809101621E-5 1.6604059300039277E-4 0.0 21.751370514149222 3.6227038472812966E-4 84-85 4.528379809101621E-5 1.6604059300039277E-4 0.0 22.75706329135324 3.6227038472812966E-4 86-87 4.528379809101621E-5 1.6604059300039277E-4 0.0 23.765141015256717 3.6227038472812966E-4 88 4.528379809101621E-5 1.6604059300039277E-4 0.0 24.536059941257857 3.6227038472812966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12550 0.0 55.97102 1 TACCTGG 5715 0.0 46.13068 2 GTATCAA 22530 0.0 40.506126 1 ACCTGGG 6740 0.0 38.13998 3 CAACGCA 27470 0.0 32.70544 5 ATCAACG 27430 0.0 32.672165 3 TATCAAC 28355 0.0 32.036526 2 TCAACGC 28855 0.0 31.140324 4 AACGCAG 29425 0.0 30.8507 6 CCTGGGG 6120 0.0 30.716017 4 GTGGTAT 4730 0.0 28.42898 1 GTACATG 25810 0.0 27.61643 1 ACGCAGA 32600 0.0 27.428087 7 TGGGGGG 13975 0.0 27.132893 6 TACATGG 25900 0.0 27.053352 2 CGCAGAG 33155 0.0 26.84141 8 ACATGGG 25670 0.0 26.161304 3 GTACTAG 2675 0.0 25.485935 1 CTGGGGG 4630 0.0 25.473198 5 CATGGGG 18360 0.0 25.007957 4 >>END_MODULE