FastQCFastQC Report
Mon 6 Jun 2016
ERR1211385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165343
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271270.3785862030610398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219580.30644729777765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126720.17685126866920398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.056.362841
GTATCAA210750.042.2286571
ATCAACG248100.034.7141533
CAACGCA249550.034.4182555
TATCAAC258550.033.663452
TCAACGC263950.032.8430944
AACGCAG270450.032.1932566
TACCTGG50550.030.9159892
GTGGTAT40700.029.981151
ACGCAGA298000.028.8539527
CGCAGAG301500.028.4386528
GCAGAGT329350.025.233529
GTACATG197000.024.3936561
TACATGG200150.023.3068892
TGGTATC50450.022.7664262
ACATGGG197350.022.6781543
CAGAGTA322100.022.18227810-11
GTACTAG28750.021.9588641
GAGTACT229450.021.92783712-13
ACCTGGG70300.021.7338123
Produced by FastQC (version 0.11.5)