FastQCFastQC Report
Mon 6 Jun 2016
ERR1211385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165343
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253300.3535071524140575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215800.30117190482018796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126570.1766419276788285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116650.056.1128121
GTATCAA213900.040.933271
ATCAACG258150.033.351963
CAACGCA259150.033.235835
TATCAAC268800.032.3098262
TCAACGC270600.031.834844
AACGCAG276250.031.3978866
TACCTGG49350.029.1404462
GTGGTAT43900.027.8494321
ACGCAGA309800.027.6184657
CGCAGAG313900.027.1828828
GTACTAG28650.024.6192151
GCAGAGT344700.024.481399
TGGTATC50350.023.8014072
GTACATG199950.023.3526081
TACATGG202450.022.4244212
TAACGCA6100.022.3427284
ACATGGG193750.022.2673723
GAGTACT240250.021.54215212-13
CAGAGTA339200.021.422110-11
Produced by FastQC (version 0.11.5)