##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211385_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7165343 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07752845886094 33.0 31.0 34.0 30.0 34.0 2 32.21269267919205 33.0 31.0 34.0 30.0 34.0 3 32.29165023921395 34.0 31.0 34.0 30.0 34.0 4 35.931511164224794 37.0 35.0 37.0 35.0 37.0 5 35.788917013463276 37.0 35.0 37.0 35.0 37.0 6 35.8251489147135 37.0 35.0 37.0 35.0 37.0 7 35.75524828329921 37.0 35.0 37.0 33.0 37.0 8 35.74355589118343 37.0 35.0 37.0 33.0 37.0 9 37.33283389225052 39.0 37.0 39.0 34.0 39.0 10-11 37.3182292878373 39.0 37.0 39.0 34.0 39.0 12-13 37.239916079383775 39.0 37.0 39.0 33.0 39.0 14-15 38.45704595020783 40.0 38.0 41.0 34.0 41.0 16-17 38.451143441423525 40.0 38.0 41.0 34.0 41.0 18-19 38.45927640867995 40.0 38.0 41.0 34.0 41.0 20-21 38.42322126100593 40.0 38.0 41.0 34.0 41.0 22-23 38.316791952597384 40.0 38.0 41.0 34.0 41.0 24-25 38.198139363321474 40.0 38.0 41.0 33.0 41.0 26-27 38.06864835360987 40.0 37.0 41.0 33.0 41.0 28-29 37.99722330110366 40.0 37.0 41.0 33.0 41.0 30-31 38.030032756282566 40.0 37.0 41.0 33.0 41.0 32-33 37.86465756070575 40.0 37.0 41.0 33.0 41.0 34-35 37.74574943586092 40.0 37.0 41.0 33.0 41.0 36-37 37.58456915460991 40.0 37.0 41.0 32.5 41.0 38-39 37.459999193339385 40.0 36.0 41.0 32.0 41.0 40-41 37.38811212526741 40.0 36.0 41.0 32.0 41.0 42-43 37.24360208576198 40.0 36.0 41.0 31.0 41.0 44-45 37.077023667952815 40.0 36.0 41.0 31.0 41.0 46-47 36.84288672572967 39.0 36.0 41.0 30.5 41.0 48-49 36.611893387378664 39.0 35.0 41.0 30.0 41.0 50-51 36.42849567703877 39.0 35.0 41.0 30.0 41.0 52-53 36.22302421530972 39.0 35.0 40.0 29.0 41.0 54-55 36.027124172562296 39.0 35.0 40.0 29.0 41.0 56-57 35.799427187226065 38.0 35.0 40.0 28.0 41.0 58-59 35.536020885531926 38.0 34.0 40.0 28.0 41.0 60-61 35.28963714647017 38.0 34.0 40.0 27.0 41.0 62-63 35.0111117499888 37.0 34.0 40.0 27.0 41.0 64-65 34.66868515575598 37.0 34.0 39.5 26.0 41.0 66-67 34.31843060408971 36.0 33.0 39.0 26.0 41.0 68-69 33.95142284186535 36.0 33.0 39.0 26.0 40.0 70-71 33.58941567765842 35.0 33.0 38.5 25.0 40.0 72-73 33.24191961222233 35.0 32.0 37.5 25.0 40.0 74-75 32.880006511900405 35.0 32.0 37.0 24.5 39.0 76-77 31.85543671810268 34.0 30.5 36.0 23.5 39.0 78-79 32.32851860685525 35.0 31.5 36.0 24.0 38.5 80-81 32.21241781447169 35.0 32.0 36.0 24.0 37.5 82-83 31.950204756422686 34.5 31.5 35.0 24.0 37.0 84-85 31.682498521005904 34.0 31.0 35.0 23.5 37.0 86-87 31.426630867496502 34.0 31.0 35.0 23.0 36.0 88-89 31.189396167078115 34.0 31.0 35.0 21.5 36.0 90-91 30.97204509260757 34.0 31.0 35.0 20.0 35.5 92-93 30.802641967034937 34.0 30.0 35.0 20.0 35.0 94-95 30.643411069086294 34.0 30.0 35.0 20.0 35.0 96-97 30.47947111533949 34.0 30.0 35.0 19.5 35.0 98-99 30.283709865668676 34.0 29.5 35.0 19.0 35.0 100 28.440275085226205 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3220999432913807 1101 2 -0.3095322629695758 1101 3 -0.3487727702457164 1101 4 -0.18916238297249066 1101 5 -0.20294385051718677 1101 6 -0.3195906335983878 1101 7 -0.2639047226089062 1101 8 -0.2895107828785086 1101 9 -0.440739193423326 1101 10-11 -0.45779052433702816 1101 12-13 -0.4654942839871197 1101 14-15 -0.6163100848376004 1101 16-17 -0.5989701509772161 1101 18-19 -0.6589012599429935 1101 20-21 -0.7375386138184226 1101 22-23 -0.7439237437720507 1101 24-25 -0.8235945499886697 1101 26-27 -0.6938932698464981 1101 28-29 -0.7804422377940767 1101 30-31 -0.6947111132642476 1101 32-33 -0.6177271277826719 1101 34-35 -0.7466950938903949 1101 36-37 -0.7526036907279661 1101 38-39 -0.8119631755749452 1101 40-41 -0.7737744204279906 1101 42-43 -0.7435287796190408 1101 44-45 -0.8111283767920128 1101 46-47 -0.7639328131108627 1101 48-49 -0.7338239156956163 1101 50-51 -0.786811902615888 1101 52-53 -0.8275231600191688 1101 54-55 -0.8674545623706607 1101 56-57 -0.7939569850313077 1101 58-59 -0.7647472323394666 1101 60-61 -0.8122234615912092 1101 62-63 -0.9361306387706918 1101 64-65 -0.9021833223216404 1101 66-67 -0.8818208456725287 1101 68-69 -0.973737740418926 1101 70-71 -0.9936118237455034 1101 72-73 -1.0669319427146036 1101 74-75 -1.0310991310344235 1101 76-77 -0.8180849792312834 1101 78-79 -0.748253536639325 1101 80-81 -0.7944124123897112 1101 82-83 -0.7343246348617178 1101 84-85 -0.827189457083584 1101 86-87 -0.9361666898480827 1101 88-89 -0.8920525652111913 1101 90-91 -0.9791085474254295 1101 92-93 -1.080040466496996 1101 94-95 -1.1519979987782563 1101 96-97 -1.1828094642221991 1101 98-99 -1.3046810412156802 1101 100 -1.2153659465554654 1102 1 -0.2739693888583048 1102 2 -0.23133532080204589 1102 3 -0.2731581878034035 1102 4 -0.1598839025385459 1102 5 -0.1443653208252087 1102 6 -0.26466940220656454 1102 7 -0.22531385628694522 1102 8 -0.2841569214322135 1102 9 -0.38440382143669893 1102 10-11 -0.42269436056439247 1102 12-13 -0.44846938434667294 1102 14-15 -0.5666258376743656 1102 16-17 -0.6288953274360054 1102 18-19 -0.5755039268082598 1102 20-21 -0.6050832668147592 1102 22-23 -0.6505633652226024 1102 24-25 -0.7062999663075331 1102 26-27 -0.7201028015707323 1102 28-29 -0.7356831486547364 1102 30-31 -0.5892577218339596 1102 32-33 -0.5671914651146537 1102 34-35 -0.6050049718956529 1102 36-37 -0.5612503373473032 1102 38-39 -0.5859865134113065 1102 40-41 -0.6705575501417869 1102 42-43 -0.6134397354018475 1102 44-45 -0.6020743927905556 1102 46-47 -0.6779660246755839 1102 48-49 -0.7240182120873939 1102 50-51 -0.6700127566675818 1102 52-53 -0.6655007343412791 1102 54-55 -0.7795468562906862 1102 56-57 -0.6758289598654699 1102 58-59 -0.7538577414079626 1102 60-61 -0.7500533539904239 1102 62-63 -0.7722984597074216 1102 64-65 -0.7467174504692125 1102 66-67 -0.782519270005416 1102 68-69 -0.899187127790583 1102 70-71 -0.9692828353934075 1102 72-73 -0.8941926688723925 1102 74-75 -0.9043909539060806 1102 76-77 -0.6840069008254694 1102 78-79 -0.6528860591815118 1102 80-81 -0.5872372204456084 1102 82-83 -0.5956437325253532 1102 84-85 -0.6246979260731464 1102 86-87 -0.73237890937963 1102 88-89 -0.7580284088656093 1102 90-91 -0.7293209173856958 1102 92-93 -0.7116126899809814 1102 94-95 -0.7046359765737975 1102 96-97 -0.8518164097522565 1102 98-99 -0.873089147701517 1102 100 -1.0127154145982118 1103 1 -0.3306198268112652 1103 2 -0.2880281831321625 1103 3 -0.2719743809473272 1103 4 -0.22250747048423847 1103 5 -0.1696711839111842 1103 6 -0.26651217635325963 1103 7 -0.2745096457138274 1103 8 -0.37514035184140937 1103 9 -0.5049909451750807 1103 10-11 -0.4425129361427764 1103 12-13 -0.5258891485486501 1103 14-15 -0.6717875319817139 1103 16-17 -0.7256946027016582 1103 18-19 -0.7201507670258351 1103 20-21 -0.6285045797843907 1103 22-23 -0.7240375697192079 1103 24-25 -0.7109654436095667 1103 26-27 -0.7545118429650728 1103 28-29 -0.8395073635258683 1103 30-31 -0.6650568377766319 1103 32-33 -0.6612909567631675 1103 34-35 -0.6066925913878976 1103 36-37 -0.6720864310440433 1103 38-39 -0.7480103970388328 1103 40-41 -0.7220512957881979 1103 42-43 -0.7131328533897872 1103 44-45 -0.7473860359663433 1103 46-47 -0.7944277019390853 1103 48-49 -0.7843062161779102 1103 50-51 -0.6502694889901477 1103 52-53 -0.7775180412640523 1103 54-55 -0.7329903633231254 1103 56-57 -0.8623359700769626 1103 58-59 -0.8797997848920218 1103 60-61 -0.8358281268625376 1103 62-63 -0.901884460774518 1103 64-65 -0.9826219048435547 1103 66-67 -0.9717004980521864 1103 68-69 -0.9015416807228647 1103 70-71 -0.8894493279163385 1103 72-73 -1.036332024614687 1103 74-75 -1.0165120060306307 1103 76-77 -0.9385770622233629 1103 78-79 -0.7677476519667721 1103 80-81 -0.705876528020493 1103 82-83 -0.7548061011765199 1103 84-85 -0.80059032006778 1103 86-87 -0.8735218480365745 1103 88-89 -0.7689332337585277 1103 90-91 -0.8993029842865319 1103 92-93 -1.0175592352657645 1103 94-95 -1.054669230616156 1103 96-97 -1.0450207285167998 1103 98-99 -1.0807654589667663 1103 100 -1.1659657888219748 1104 1 -0.375783274683517 1104 2 -0.298344697916324 1104 3 -0.3803892292041624 1104 4 -0.21268039359546265 1104 5 -0.23261707750982907 1104 6 -0.36611122046885924 1104 7 -0.31301708656325644 1104 8 -0.3351889821120153 1104 9 -0.46056740199307455 1104 10-11 -0.46666626083691654 1104 12-13 -0.5562546767932233 1104 14-15 -0.708613117913778 1104 16-17 -0.6470786843001903 1104 18-19 -0.6732763766459868 1104 20-21 -0.6906184289608817 1104 22-23 -0.6953801971472444 1104 24-25 -0.8152883472129417 1104 26-27 -0.9149922584821724 1104 28-29 -0.8191476179638997 1104 30-31 -0.763396406653257 1104 32-33 -0.7096216426032527 1104 34-35 -0.7763308950414398 1104 36-37 -0.6673845178818212 1104 38-39 -0.79750946344911 1104 40-41 -0.8517680140113129 1104 42-43 -0.82643145579204 1104 44-45 -0.9070002683326379 1104 46-47 -0.8369919036502012 1104 48-49 -0.8303454269462449 1104 50-51 -0.8323922908740471 1104 52-53 -0.7171578080059575 1104 54-55 -0.702156995154354 1104 56-57 -0.7507866291634357 1104 58-59 -0.8828346481977647 1104 60-61 -0.9073398803391157 1104 62-63 -0.8894894896182493 1104 64-65 -0.817661652077625 1104 66-67 -0.8555563629464231 1104 68-69 -0.8287671426407641 1104 70-71 -0.8523609086091497 1104 72-73 -0.8394687815748085 1104 74-75 -0.7693011053255674 1104 76-77 -0.7080873456444827 1104 78-79 -0.7077963867436878 1104 80-81 -0.8236861453880451 1104 82-83 -0.767024282488002 1104 84-85 -0.6897679947326623 1104 86-87 -0.5851924162722284 1104 88-89 -0.6769762137211863 1104 90-91 -0.721789259124197 1104 92-93 -0.8095660699785796 1104 94-95 -0.8402966516790542 1104 96-97 -0.9244918569062861 1104 98-99 -0.9400102615195074 1104 100 -0.8609783992503104 1105 1 -0.4101493563407921 1105 2 -0.35464897058628253 1105 3 -0.4155179244908709 1105 4 -0.2764030327131408 1105 5 -0.29610178117511765 1105 6 -0.3748663638741192 1105 7 -0.3247223584265413 1105 8 -0.34261616098388714 1105 9 -0.505791745975884 1105 10-11 -0.5195670736135725 1105 12-13 -0.5650762523190878 1105 14-15 -0.7911360471635689 1105 16-17 -0.7340029110099735 1105 18-19 -0.7204406402323755 1105 20-21 -0.8141714967013058 1105 22-23 -0.7595188843671963 1105 24-25 -0.765738967133089 1105 26-27 -0.8490751562783885 1105 28-29 -0.834825598427436 1105 30-31 -0.8028550526581881 1105 32-33 -0.7251715039770446 1105 34-35 -0.7380051539504535 1105 36-37 -0.8422962241705036 1105 38-39 -0.759313366675137 1105 40-41 -0.7882007786043417 1105 42-43 -0.8764065437468034 1105 44-45 -0.8257936102072492 1105 46-47 -0.937835693263743 1105 48-49 -0.8538007106724095 1105 50-51 -0.9563297158837045 1105 52-53 -0.8903746478706509 1105 54-55 -0.95693722685332 1105 56-57 -0.9736389397132612 1105 58-59 -0.9769469320391693 1105 60-61 -1.0530182336359744 1105 62-63 -1.0054692956093518 1105 64-65 -0.9475764427147553 1105 66-67 -0.9627415390932228 1105 68-69 -0.894866255297444 1105 70-71 -0.9712206826043612 1105 72-73 -1.1060580005906644 1105 74-75 -1.0246597370950283 1105 76-77 -0.9742752604215603 1105 78-79 -0.859291278927067 1105 80-81 -0.7285033214806198 1105 82-83 -0.76409872713581 1105 84-85 -0.7678784415225657 1105 86-87 -0.7643710305524216 1105 88-89 -0.799111328519956 1105 90-91 -0.8005458938627754 1105 92-93 -0.8757236496801752 1105 94-95 -0.8742492272794848 1105 96-97 -0.9612577989205349 1105 98-99 -0.9684760862607256 1105 100 -0.95741140110092 1106 1 -0.4048378845819549 1106 2 -0.39541823865818415 1106 3 -0.4215291390547158 1106 4 -0.25092689868436935 1106 5 -0.33390046502643145 1106 6 -0.3550595654488973 1106 7 -0.3629687986466621 1106 8 -0.3853783310486918 1106 9 -0.49764223881321357 1106 10-11 -0.5324381384583248 1106 12-13 -0.5756209327650836 1106 14-15 -0.7732753635134131 1106 16-17 -0.7624780069192738 1106 18-19 -0.7987887550936463 1106 20-21 -0.6975375631265877 1106 22-23 -0.7630241090895211 1106 24-25 -0.8427858405615396 1106 26-27 -0.8491794621392685 1106 28-29 -0.8610222196898434 1106 30-31 -0.7871719951988112 1106 32-33 -0.7921641994236879 1106 34-35 -0.7611522094988317 1106 36-37 -0.8208804752876446 1106 38-39 -0.8580198482763493 1106 40-41 -0.898499624100559 1106 42-43 -0.8258868553981742 1106 44-45 -0.8912011978187806 1106 46-47 -0.8418577775489879 1106 48-49 -0.9118757713790515 1106 50-51 -0.8991955182889839 1106 52-53 -0.9614095883134937 1106 54-55 -0.9799775967686912 1106 56-57 -1.0269074893633885 1106 58-59 -1.0065275892514052 1106 60-61 -1.14516750012735 1106 62-63 -1.0969087164895583 1106 64-65 -1.1867854334040118 1106 66-67 -1.0319687507546504 1106 68-69 -1.0784129149954111 1106 70-71 -1.0092145975719617 1106 72-73 -1.0481791139288816 1106 74-75 -1.0664811787519994 1106 76-77 -1.0512132929517009 1106 78-79 -0.8770259244577652 1106 80-81 -0.8393315091180185 1106 82-83 -0.874656653483207 1106 84-85 -0.8505511860374178 1106 86-87 -0.832273472468021 1106 88-89 -0.9146262752849061 1106 90-91 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0.7635712441728728 2116 18-19 0.6491874049885595 2116 20-21 0.7485975515420478 2116 22-23 0.7817558164924847 2116 24-25 0.825587420339545 2116 26-27 0.8448349683629601 2116 28-29 0.8265843562631545 2116 30-31 0.5864102097296069 2116 32-33 0.6795684497747629 2116 34-35 0.7281290273023018 2116 36-37 0.7797350057658647 2116 38-39 0.6649950416253674 2116 40-41 0.8091428370198983 2116 42-43 0.8911212877138297 2116 44-45 0.8957076700884983 2116 46-47 0.8058765871086138 2116 48-49 0.7524064307126039 2116 50-51 0.9366481613827133 2116 52-53 0.9170254669405224 2116 54-55 0.9464226457753213 2116 56-57 0.9623874388032405 2116 58-59 0.9468461208052688 2116 60-61 0.9643039304253165 2116 62-63 0.9267953711414876 2116 64-65 0.8161253115008336 2116 66-67 0.8101962028168472 2116 68-69 0.8014877073608773 2116 70-71 0.7498965629200001 2116 72-73 0.9527332826077384 2116 74-75 0.8746787497928636 2116 76-77 0.7394287867805112 2116 78-79 0.7659969359856547 2116 80-81 0.7730248595930149 2116 82-83 0.734449542657515 2116 84-85 0.7129641419879995 2116 86-87 0.7708335416086705 2116 88-89 0.7274678377825303 2116 90-91 0.6036773016647707 2116 92-93 0.7486999474706906 2116 94-95 0.810810805804266 2116 96-97 0.8995673902643411 2116 98-99 0.9626176197341643 2116 100 0.5931005553398911 2201 1 0.6927895871173391 2201 2 0.6511662125426696 2201 3 0.6263827798251853 2201 4 0.35983026604837676 2201 5 0.5134576654046441 2201 6 0.5077287594659268 2201 7 0.5404065402238061 2201 8 0.5706048478353125 2201 9 0.7325100998880387 2201 10-11 0.6179587705149743 2201 12-13 0.6476329580199192 2201 14-15 0.8188386001699968 2201 16-17 0.7991089254314332 2201 18-19 0.7953215666697915 2201 20-21 0.8090178890727273 2201 22-23 0.7886554186039447 2201 24-25 0.7721458138103827 2201 26-27 0.7853588827267615 2201 28-29 0.7922895528412113 2201 30-31 0.9675918050347221 2201 32-33 0.7760066493455753 2201 34-35 0.883939985319742 2201 36-37 0.9295304557825901 2201 38-39 0.990589357719692 2201 40-41 0.9739154572703512 2201 42-43 2.803576014969167E-4 2201 44-45 0.7794682543661295 2201 46-47 0.908719157297881 2201 48-49 0.9248840689153113 2201 50-51 0.925357897237312 2201 52-53 0.9160751992676808 2201 54-55 0.991585166872234 2201 56-57 0.9941594455794771 2201 58-59 1.0250158659732875 2201 60-61 0.99770150416051 2201 62-63 0.9485215462483083 2201 64-65 0.9802816636986549 2201 66-67 0.9916595888970647 2201 68-69 0.9503274146704968 2201 70-71 0.9755046544708605 2201 72-73 1.0037968316682395 2201 74-75 0.9422990953265611 2201 76-77 1.0705181221257654 2201 78-79 0.872853224492836 2201 80-81 0.7313677798690392 2201 82-83 0.7457296678505969 2201 84-85 0.8229984963634003 2201 86-87 0.8068567434970255 2201 88-89 0.8120130012273954 2201 90-91 0.15321701125453302 2201 92-93 0.4895560769990972 2201 94-95 0.7658859417495876 2201 96-97 0.7399300141747283 2201 98-99 0.7384572579186646 2201 100 0.939774046197396 2202 1 0.6860313364099326 2202 2 0.6304686035666549 2202 3 0.612357189158331 2202 4 0.387732863773131 2202 5 0.38416466545394456 2202 6 0.4536816205716576 2202 7 0.5463710078373865 2202 8 0.5440106069811321 2202 9 0.7187434414669625 2202 10-11 0.7158214481082013 2202 12-13 0.6994511077286987 2202 14-15 0.8970182823502526 2202 16-17 0.8552700586984585 2202 18-19 0.8014930359153496 2202 20-21 0.8016383122329529 2202 22-23 0.8043595167128856 2202 24-25 0.8148500781160806 2202 26-27 0.8288765333845376 2202 28-29 0.9098119792977357 2202 30-31 0.9955686240486372 2202 32-33 0.6383536980961964 2202 34-35 0.9591285879383804 2202 36-37 1.1098619567518924 2202 38-39 1.0873020166702219 2202 40-41 1.0891489408514516 2202 42-43 1.1251934474727037 2202 44-45 1.1361898183043593 2202 46-47 1.206113019287372 2202 48-49 1.1423468676193664 2202 50-51 1.1261792925624476 2202 52-53 1.1665183096876817 2202 54-55 1.1358349749373744 2202 56-57 1.161465482464756 2202 58-59 1.145801725652639 2202 60-61 1.176334432162399 2202 62-63 1.2387096046454005 2202 64-65 1.158295341852515 2202 66-67 1.1818484025915552 2202 68-69 1.2452769023547745 2202 70-71 1.2485797657260136 2202 72-73 1.1807149681796858 2202 74-75 1.1358514113144054 2202 76-77 1.185831319355767 2202 78-79 1.055257272498281 2202 80-81 1.0053955373932482 2202 82-83 1.0453922426544224 2202 84-85 1.024062221494571 2202 86-87 0.9876297957143017 2202 88-89 1.0147379262077187 2202 90-91 1.0820255067268683 2202 92-93 1.1158854278466777 2202 94-95 1.0016576117099483 2202 96-97 0.8448865092011566 2202 98-99 0.8607112230028058 2202 100 1.1226771467747092 2203 1 0.6252726872207859 2203 2 0.5895332770843353 2203 3 0.5729513651412077 2203 4 0.36127080263511857 2203 5 0.47313812062292726 2203 6 0.487613711396655 2203 7 0.4921166758543549 2203 8 0.5081181281224971 2203 9 0.6486730050004965 2203 10-11 0.6659580950511241 2203 12-13 0.6292913711183061 2203 14-15 0.7944638128548931 2203 16-17 0.7453437689181044 2203 18-19 0.7746312077464523 2203 20-21 0.8085406800806396 2203 22-23 0.7773706438014045 2203 24-25 0.7890575684774035 2203 26-27 0.8293712367028974 2203 28-29 0.7841701414985351 2203 30-31 0.8958130506610829 2203 32-33 0.9509813675246619 2203 34-35 0.9429048449827988 2203 36-37 0.9719925215135916 2203 38-39 0.932849777813594 2203 40-41 0.9347176100117167 2203 42-43 0.9839309660093036 2203 44-45 1.0250060458249663 2203 46-47 1.0349325068300388 2203 48-49 0.9925475286990846 2203 50-51 1.0204754242935294 2203 52-53 1.027772549927711 2203 54-55 0.951474308883796 2203 56-57 1.019181557758401 2203 58-59 1.0071440206564333 2203 60-61 0.9782231086518749 2203 62-63 0.9673337080076081 2203 64-65 1.0962314464419691 2203 66-67 1.0919926993619455 2203 68-69 1.067434180840209 2203 70-71 1.1358425331797903 2203 72-73 1.0975928199206209 2203 74-75 1.1170597104383688 2203 76-77 1.1124604338722754 2203 78-79 0.9311122539183216 2203 80-81 0.9375217439630532 2203 82-83 0.9145698788583729 2203 84-85 0.8278817641213543 2203 86-87 0.8532799964009321 2203 88-89 0.9040412490512288 2203 90-91 0.9890024584762749 2203 92-93 0.9243951120664917 2203 94-95 0.9781323006834413 2203 96-97 0.9692337902919839 2203 98-99 0.9572483987660867 2203 100 1.2722534029824892 2204 1 0.6700797719618876 2204 2 0.6777729810125521 2204 3 0.631585233207737 2204 4 0.4044503250228857 2204 5 0.4875216721506135 2204 6 0.5395164577766565 2204 7 0.55128829070145 2204 8 0.5285229666351015 2204 9 0.6975938638020196 2204 10-11 0.6771341873171082 2204 12-13 0.7227626373586844 2204 14-15 0.8610553486355386 2204 16-17 0.8814234673323469 2204 18-19 0.8347856706419279 2204 20-21 0.8532205450777752 2204 22-23 0.8304285499322575 2204 24-25 0.8182115896283264 2204 26-27 0.8753851747055847 2204 28-29 0.8857826881177999 2204 30-31 1.0073909201096853 2204 32-33 1.0229257551534943 2204 34-35 1.0347564931106632 2204 36-37 1.0039002850732928 2204 38-39 1.0090097600542975 2204 40-41 1.023781612843898 2204 42-43 1.087410744895358 2204 44-45 1.1278819505173487 2204 46-47 1.0928480365005413 2204 48-49 1.0525146865361634 2204 50-51 1.0790290667167568 2204 52-53 1.0683356198150946 2204 54-55 0.9957979138415354 2204 56-57 1.0534755027251705 2204 58-59 1.0292431083890463 2204 60-61 1.0495247960086331 2204 62-63 1.0852706625124 2204 64-65 1.0295141454966696 2204 66-67 1.0330553836739256 2204 68-69 1.0770005013766735 2204 70-71 1.0893568639188942 2204 72-73 1.0871913470807542 2204 74-75 1.1166194204713449 2204 76-77 1.1125929124798866 2204 78-79 0.9770287351074884 2204 80-81 0.9791522512800341 2204 82-83 0.9186998770725978 2204 84-85 0.9276966744991348 2204 86-87 0.9329579346399406 2204 88-89 0.930121632236645 2204 90-91 0.9640927878512109 2204 92-93 0.9495725371746779 2204 94-95 0.9560549197497039 2204 96-97 0.9322696852812022 2204 98-99 0.961831554379664 2204 100 1.1564773938736899 2205 1 0.6688425313045769 2205 2 0.4902527262550933 2205 3 0.55820609987137 2205 4 0.42371323923394755 2205 5 0.513766452720148 2205 6 0.5298779562187192 2205 7 0.5646513664433712 2205 8 0.5602691984087897 2205 9 0.7287430704072619 2205 10-11 0.7224721029675862 2205 12-13 0.749816496768382 2205 14-15 0.9143940480674431 2205 16-17 0.884898448740465 2205 18-19 0.8515103590712769 2205 20-21 0.8499173543822849 2205 22-23 0.8514785008292947 2205 24-25 0.859107688432502 2205 26-27 0.9101204459062018 2205 28-29 0.942458681222007 2205 30-31 1.0323403980797536 2205 32-33 1.0706557085439528 2205 34-35 1.0631113141632653 2205 36-37 1.0219826586417327 2205 38-39 1.0677811724892337 2205 40-41 1.0588779826747654 2205 42-43 1.0930055820568825 2205 44-45 1.1368091658888346 2205 46-47 1.153480428890191 2205 48-49 1.1467070302057536 2205 50-51 1.1770416951970049 2205 52-53 1.1376112187367866 2205 54-55 1.1415570345210497 2205 56-57 1.081851433653469 2205 58-59 1.1387280033141494 2205 60-61 1.1522921793924965 2205 62-63 1.165737360301975 2205 64-65 1.160635160203391 2205 66-67 1.157099494532062 2205 68-69 1.227640136089562 2205 70-71 1.2028278152740697 2205 72-73 1.2154202070626212 2205 74-75 1.2523590599525534 2205 76-77 1.1682961214972103 2205 78-79 1.0662723431357435 2205 80-81 1.0163597339161683 2205 82-83 0.9120499374842623 2205 84-85 0.9787034958145959 2205 86-87 1.0342369191567649 2205 88-89 1.113278341841184 2205 90-91 1.1128303030073532 2205 92-93 1.111254343204461 2205 94-95 1.1858777060610635 2205 96-97 1.1782267018485335 2205 98-99 1.2088497002941914 2205 100 1.272921033699042 2206 1 0.6809034094557518 2206 2 0.6306695104130142 2206 3 0.6412353148392356 2206 4 0.30261815538565173 2206 5 0.4839080236516722 2206 6 0.49475054439579225 2206 7 0.5385000880316326 2206 8 0.5464505777900257 2206 9 0.6947677447007408 2206 10-11 0.7082205969266511 2206 12-13 0.7755992694691756 2206 14-15 0.980061834466234 2206 16-17 0.9131766624141093 2206 18-19 0.9105465695249677 2206 20-21 0.8756481912150136 2206 22-23 0.9318621013036861 2206 24-25 0.9425915082910876 2206 26-27 0.9116203488900396 2206 28-29 0.9497216867787088 2206 30-31 1.0213815558390422 2206 32-33 1.1011008151942505 2206 34-35 1.0963986862000894 2206 36-37 1.1419252593921243 2206 38-39 1.0988195832586953 2206 40-41 1.1312963173202704 2206 42-43 1.209397671889036 2206 44-45 1.2446197753598724 2206 46-47 1.2889511594747916 2206 48-49 1.2335344649131343 2206 50-51 1.1991396627189062 2206 52-53 1.21839154877987 2206 54-55 1.2383044643254166 2206 56-57 1.2205522500123323 2206 58-59 1.2391810187520278 2206 60-61 1.1874473417490705 2206 62-63 1.1925630339383488 2206 64-65 1.2151054192350372 2206 66-67 1.3128759891231354 2206 68-69 1.3375020940182054 2206 70-71 1.3884553184332802 2206 72-73 1.3252254424424166 2206 74-75 1.2926816159710626 2206 76-77 1.2784414020681574 2206 78-79 1.1900014984769811 2206 80-81 1.1541909393981271 2206 82-83 1.1490648024259613 2206 84-85 1.1752648848198781 2206 86-87 1.16474930501008 2206 88-89 1.201645797566595 2206 90-91 1.2441106953765377 2206 92-93 1.315895092048379 2206 94-95 1.3062946768002917 2206 96-97 1.2806758624128314 2206 98-99 1.2973714596746682 2206 100 1.5315291499919006 2207 1 0.6755711547654641 2207 2 0.6206086897191341 2207 3 0.6206086870582226 2207 4 0.3823688913049281 2207 5 0.4931061629924187 2207 6 0.5181946251089897 2207 7 0.5647626078034662 2207 8 0.5735604491310013 2207 9 0.7136323438008887 2207 10-11 0.7246772993413018 2207 12-13 0.7564906488951308 2207 14-15 0.892242298117786 2207 16-17 0.9021110685095906 2207 18-19 0.8901256387578584 2207 20-21 0.8528971332806208 2207 22-23 0.9065307986736784 2207 24-25 0.891065504495046 2207 26-27 0.9306973237696283 2207 28-29 0.9141959433200455 2207 30-31 0.9793331237621743 2207 32-33 1.0206998633491366 2207 34-35 0.9728599911358486 2207 36-37 1.0175195798774794 2207 38-39 1.060565586488174 2207 40-41 1.0135246242415548 2207 42-43 1.0889890318434823 2207 44-45 1.1002254470572126 2207 46-47 1.0936312439159082 2207 48-49 1.123179428908763 2207 50-51 1.1688056509305724 2207 52-53 1.1362601243004846 2207 54-55 1.11056585668576 2207 56-57 1.1241810698628072 2207 58-59 1.0876057175979739 2207 60-61 1.0501438005510337 2207 62-63 1.1776000889846543 2207 64-65 1.293472799032493 2207 66-67 1.2299418128847606 2207 68-69 1.2674467741169906 2207 70-71 1.2933581688407614 2207 72-73 1.2942322051183481 2207 74-75 1.2105405991706988 2207 76-77 1.1665407024921173 2207 78-79 1.1342637934119821 2207 80-81 1.0442229132037326 2207 82-83 1.0065855317005372 2207 84-85 1.0297079582497268 2207 86-87 1.0323291964931407 2207 88-89 1.1264802807233814 2207 90-91 1.186495629659273 2207 92-93 1.1696769676895826 2207 94-95 1.2246936538823547 2207 96-97 1.1918168591320786 2207 98-99 1.249892429205513 2207 100 1.4618821760912617 2208 1 0.6775760061834006 2208 2 0.6386226111637185 2208 3 0.6064495806880501 2208 4 0.3783010495695862 2208 5 0.4466704716922365 2208 6 0.5151493650955246 2208 7 0.561361347810788 2208 8 0.5166437206973526 2208 9 0.7036912190667053 2208 10-11 0.6979454096084936 2208 12-13 0.7376583037439204 2208 14-15 0.9156052222697255 2208 16-17 0.8666358587034111 2208 18-19 0.8884484793187823 2208 20-21 0.8703027910465693 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0.9567011156499419 2311 42-43 1.017644493681935 2311 44-45 0.9794908882102362 2311 46-47 1.0380690613177421 2311 48-49 1.0374870316479203 2311 50-51 1.0439192766924918 2311 52-53 1.0474792230708374 2311 54-55 1.0742263857266678 2311 56-57 1.1320819835497389 2311 58-59 1.1295246882022951 2311 60-61 1.0382827635531626 2311 62-63 1.0721158608733887 2311 64-65 1.0716209091076365 2311 66-67 1.1389972236638002 2311 68-69 1.162290146060343 2311 70-71 1.1383059474039428 2311 72-73 1.1760170164103414 2311 74-75 1.0329235534893328 2311 76-77 0.9682642559361447 2311 78-79 0.9823491647875073 2311 80-81 0.9716924365311783 2311 82-83 0.9551286923572206 2311 84-85 1.0326037614635162 2311 86-87 1.0432917892448081 2311 88-89 1.088717905751917 2311 90-91 1.0681257263069952 2311 92-93 1.149256732397017 2311 94-95 1.1722692322090111 2311 96-97 1.1956302787237796 2311 98-99 1.2088321698287139 2311 100 1.2815050012749545 2312 1 0.150021403562711 2312 2 0.16966042611294796 2312 3 0.3429277583935857 2312 4 0.23153874919229622 2312 5 0.3523168714720626 2312 6 0.42743022538648034 2312 7 0.45482617097945877 2312 8 0.3032088480137105 2312 9 0.40079864890884664 2312 10-11 0.3225197958904431 2312 12-13 0.4866047017077548 2312 14-15 0.8303091181585458 2312 16-17 0.8836349341044141 2312 18-19 0.9196940633035666 2312 20-21 0.8741143036494634 2312 22-23 0.4897975570296609 2312 24-25 0.525745803657415 2312 26-27 0.8141522622554831 2312 28-29 0.9414666220505552 2312 30-31 0.8606675030368365 2312 32-33 0.7306790728142616 2312 34-35 0.8994500676329267 2312 36-37 0.9910165279449572 2312 38-39 1.0083168184452447 2312 40-41 0.9939319153201254 2312 42-43 0.8779026442866993 2312 44-45 0.8472316094535586 2312 46-47 1.0045273640565568 2312 48-49 1.0928329047698568 2312 50-51 1.0891039557021216 2312 52-53 1.1670584622795204 2312 54-55 1.1668205343524747 2312 56-57 1.1487311854897442 2312 58-59 1.233845277202974 2312 60-61 1.186100071627358 2312 62-63 1.2241435545611736 2312 64-65 1.272143431572168 2312 66-67 1.2834608873503228 2312 68-69 1.2806841763552583 2312 70-71 1.1737014725951482 2312 72-73 1.2618697115376705 2312 74-75 1.3128592702125559 2312 76-77 1.2404843530938372 2312 78-79 1.1896466036044728 2312 80-81 1.021848440781703 2312 82-83 1.0061947728019476 2312 84-85 0.8130713674145333 2312 86-87 1.030805848428944 2312 88-89 0.7028484706901104 2312 90-91 1.0530728513820549 2312 92-93 1.1599020460951763 2312 94-95 1.2589451560811895 2312 96-97 1.3658100606007046 2312 98-99 1.3311409853779637 2312 100 1.4547717881460542 2313 1 -0.30224627017606664 2313 2 -0.005084849052735763 2313 3 0.2803916181836925 2313 4 0.059155597370910584 2313 5 0.1627079706314376 2313 6 0.3144685985323008 2313 7 0.09503239454897283 2313 8 0.048432851056951165 2313 9 0.24811632135202188 2313 10-11 0.3680769781879576 2313 12-13 0.5891414029472699 2313 14-15 0.6460161067678243 2313 16-17 0.7789225188635598 2313 18-19 0.6458450998269214 2313 20-21 0.8283214494976505 2313 22-23 0.8337851822305069 2313 24-25 0.749253068984082 2313 26-27 0.9267360174975963 2313 28-29 0.8174472574358873 2313 30-31 0.8251529411159026 2313 32-33 0.9343498730701043 2313 34-35 0.9674459156758672 2313 36-37 0.8277012884505481 2313 38-39 0.6052949138414263 2313 40-41 0.8088068671501887 2313 42-43 0.817287390144422 2313 44-45 0.9736522540715384 2313 46-47 0.9612769903405862 2313 48-49 0.9236683332026061 2313 50-51 0.8692523628178179 2313 52-53 0.8604048465188683 2313 54-55 0.9316827208583405 2313 56-57 0.9483616068695753 2313 58-59 0.9012959215502931 2313 60-61 0.9273025154814363 2313 62-63 0.974843873941289 2313 64-65 1.0251624177240686 2313 66-67 1.043809501816149 2313 68-69 1.0845137201276884 2313 70-71 0.9624407338778909 2313 72-73 1.0186530150819664 2313 74-75 0.6088191559466054 2313 76-77 0.8688700089146089 2313 78-79 0.7068812480257662 2313 80-81 0.8221185499464454 2313 82-83 0.8163043296714392 2313 84-85 0.8498832857219192 2313 86-87 0.8828112921898033 2313 88-89 0.8725775484124334 2313 90-91 0.9602471423301928 2313 92-93 0.7427275779427518 2313 94-95 0.7926398623077944 2313 96-97 0.9608504362253818 2313 98-99 1.09287821151376 2313 100 1.176542922935166 2314 1 -0.3553614639569034 2314 2 -0.17027552336666574 2314 3 0.12873800236919664 2314 4 -0.12626755863072248 2314 5 0.18202850177247853 2314 6 0.19034880475040694 2314 7 0.16231137067141788 2314 8 0.19296005987276033 2314 9 0.30352102269225156 2314 10-11 0.4467924613926897 2314 12-13 0.5486320685221173 2314 14-15 0.6118804089015342 2314 16-17 0.7929064454025294 2314 18-19 0.8311904823959608 2314 20-21 0.48076992482261005 2314 22-23 0.7023704252356353 2314 24-25 0.8640406437554375 2314 26-27 0.816866390341545 2314 28-29 0.7432911615720315 2314 30-31 0.7307728503204345 2314 32-33 0.812603261712546 2314 34-35 0.8599048517033197 2314 36-37 0.8142040508310515 2314 38-39 0.8333672939715484 2314 40-41 0.8623296389437556 2314 42-43 0.6911262729889458 2314 44-45 0.7720551403352403 2314 46-47 0.8847738548461521 2314 48-49 0.752397901251129 2314 50-51 0.8235272239045841 2314 52-53 0.9202366907195056 2314 54-55 0.9499837795615136 2314 56-57 0.8545677985586408 2314 58-59 0.8639814061197981 2314 60-61 0.9620996012107383 2314 62-63 0.626592933014436 2314 64-65 0.8593249781382539 2314 66-67 0.8903048631386596 2314 68-69 0.9294831406426027 2314 70-71 1.0266067526968925 2314 72-73 1.033216012775739 2314 74-75 1.038356467380332 2314 76-77 0.8379398229419941 2314 78-79 0.850122247934685 2314 80-81 0.8446399462926237 2314 82-83 0.8733116352861856 2314 84-85 0.7657113560944531 2314 86-87 0.8294469904008075 2314 88-89 0.8650118707789467 2314 90-91 0.9606256337271866 2314 92-93 1.017542935499371 2314 94-95 1.0637722710631685 2314 96-97 1.108845915452438 2314 98-99 1.1281800765346013 2314 100 1.222892896680687 2315 1 -0.0787914408751682 2315 2 0.07852913888538637 2315 3 0.147449530820829 2315 4 -0.02872821956842131 2315 5 0.27335764657364336 2315 6 0.37776784372313443 2315 7 0.4228939484822689 2315 8 0.3617229722359241 2315 9 0.5766606641956429 2315 10-11 0.5656826164143638 2315 12-13 0.6031009533977212 2315 14-15 0.7696387720061821 2315 16-17 0.7475474091095151 2315 18-19 0.7476584778931468 2315 20-21 0.782231321111226 2315 22-23 0.8554716341344104 2315 24-25 0.8451550049805263 2315 26-27 0.9442461745561417 2315 28-29 0.9109706730288138 2315 30-31 0.8454993207225954 2315 32-33 0.9270119360627831 2315 34-35 0.9122211811491638 2315 36-37 0.9158634138201833 2315 38-39 0.8843546413540153 2315 40-41 0.891737211127726 2315 42-43 0.942343912094465 2315 44-45 0.8270499672667455 2315 46-47 0.7773834593630085 2315 48-49 0.918796173434508 2315 50-51 0.8265434159598541 2315 52-53 0.8434729536907781 2315 54-55 0.8133645162836913 2315 56-57 0.8892007214896509 2315 58-59 0.9260784270051943 2315 60-61 0.9743833759990039 2315 62-63 0.926505630742561 2315 64-65 0.9760366106765375 2315 66-67 0.9922988761900626 2315 68-69 0.8784890694042389 2315 70-71 0.5704734138151863 2315 72-73 0.7951761004586473 2315 74-75 0.9906896672195273 2315 76-77 0.9322856035046705 2315 78-79 0.7944811048611555 2315 80-81 0.7980179992064862 2315 82-83 0.7368149373206165 2315 84-85 0.6695315397364077 2315 86-87 0.6376208768777083 2315 88-89 0.7063668529349947 2315 90-91 0.8454520576262006 2315 92-93 0.8860919145641084 2315 94-95 0.8968681942603212 2315 96-97 0.9792956637290224 2315 98-99 0.8981904218524548 2315 100 0.8797322895802573 2316 1 0.49947469240709097 2316 2 0.49914654689722937 2316 3 0.4887918120818284 2316 4 0.31694000101905573 2316 5 0.41678829932265415 2316 6 0.43878071589038825 2316 7 0.534246660080214 2316 8 0.5086649874460605 2316 9 0.5186399008323264 2316 10-11 0.5174457490167228 2316 12-13 0.6625298432602378 2316 14-15 0.8244301545121786 2316 16-17 0.7988900792697038 2316 18-19 0.8503690036258647 2316 20-21 0.9012290095298141 2316 22-23 0.9095155898776071 2316 24-25 0.9466091897066917 2316 26-27 1.0392254517191049 2316 28-29 0.982514168513049 2316 30-31 0.83839842666751 2316 32-33 0.8536413995799847 2316 34-35 0.7410967096947658 2316 36-37 0.7160948741598645 2316 38-39 0.6754067150081724 2316 40-41 0.7824620420162987 2316 42-43 0.8920677257087917 2316 44-45 0.8352966359268663 2316 46-47 0.8483287666635135 2316 48-49 0.6970299873070971 2316 50-51 0.7930145192523383 2316 52-53 0.9429490509265577 2316 54-55 0.9798178360489729 2316 56-57 0.974084303719124 2316 58-59 0.9186543322551515 2316 60-61 0.9733765533453536 2316 62-63 1.0275375358762844 2316 64-65 1.0329872612433846 2316 66-67 0.9696530138220751 2316 68-69 0.9395991993597832 2316 70-71 0.8993708880462563 2316 72-73 0.9375041111780575 2316 74-75 0.9831763579134858 2316 76-77 0.7620498201420709 2316 78-79 0.7370606938983073 2316 80-81 0.7856787241349039 2316 82-83 0.7315173900498735 2316 84-85 0.7938470253682333 2316 86-87 0.7168079833506944 2316 88-89 0.7408319383325441 2316 90-91 0.8113671551664616 2316 92-93 0.8587172584419811 2316 94-95 0.7532485156552156 2316 96-97 0.935608646312236 2316 98-99 0.9419598642550788 2316 100 0.7068275651175746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 12.0 12 34.0 13 217.0 14 913.0 15 2724.0 16 6551.0 17 12769.0 18 21174.0 19 29714.0 20 40158.0 21 52102.0 22 61789.0 23 62238.0 24 63006.0 25 67370.0 26 77618.0 27 93721.0 28 114396.0 29 139479.0 30 169706.0 31 210154.0 32 264765.0 33 342071.0 34 470414.0 35 659274.0 36 1000750.0 37 1537745.0 38 1430015.0 39 234410.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.621678802478286 20.142887382982085 13.25578868998582 23.97964512455381 2 15.71157385090621 21.306324248026122 39.34704023897254 23.635061662095126 3 18.262363713781742 26.48086769886661 30.91653811966852 24.340230467683124 4 12.36178385058628 17.486609340004538 39.00838069534005 31.143226114069133 5 12.620304480820717 37.84991561118424 35.550838521830066 13.978941386164982 6 29.67405747359198 38.30045540039046 18.169137192734528 13.856349933283028 7 25.793798384916695 33.093869965739174 23.310757485317836 17.80157416402629 8 25.36551095053502 35.263991295843084 20.87225231783347 18.498245435788423 9 25.942616184271987 15.499893861866349 21.064016297681388 37.49347365618028 10-11 24.257249079555617 26.348176605298125 29.05092872039076 20.343645594755493 12-13 24.957871773184376 24.42904032356376 28.82991444981607 21.783173453435793 14-15 22.257693127074702 25.90537742065433 26.66181739723325 25.17511205503772 16-17 21.46233667979306 28.49018123162027 27.592581699093284 22.454900389493385 18-19 21.15240680034438 27.738560456910438 29.756761120856325 21.35227162188886 20-21 22.001330990295436 27.09070460912435 29.598959100553685 21.309005300026524 22-23 21.96189505246958 26.990573025643926 29.322883030364565 21.724648891521934 24-25 21.679009185748225 27.067065494040126 29.631466145565476 21.62245917464617 26-27 21.65646408175131 27.213917662816044 29.379655483249156 21.749962772183487 28-29 21.408019889296426 27.558158732808195 29.40631890450327 21.627502473392106 30-31 21.862127817309062 27.270484767416324 29.34259456519608 21.524792850078537 32-33 21.80950210151595 27.44223064416712 28.999691807437717 21.748575446879215 34-35 21.93751354921907 27.299277174900055 29.323064613145977 21.440144662734898 36-37 21.91809574598778 27.020634863020582 29.258179396687478 21.80308999430416 38-39 21.692755290165632 27.02274918663169 29.523732417442638 21.760763105760038 40-41 21.64634520773078 26.68349491691383 29.803154968953287 21.867004906402105 42-43 21.45349692845687 26.914837591280477 29.387933458191277 22.24373202207137 44-45 21.296589145685495 27.249393476088958 29.42986473211756 22.02415264610799 46-47 21.378744415092836 26.960430742126242 29.15417327322128 22.50665156955965 48-49 21.412904280679925 26.9614871886236 29.118195134992437 22.50741339570404 50-51 21.22489815638509 27.114461036115582 28.899760954596026 22.760879852903297 52-53 20.978283771918225 27.3191540918588 28.820600493414545 22.88196164280843 54-55 20.90925801833484 27.181084918636895 28.851133718215827 23.05852334481244 56-57 21.185262059882216 26.997910287073495 28.567937442315873 23.24889021072842 58-59 20.91558157112822 27.11259268181519 28.647903976241523 23.323921770815065 60-61 21.00650648174426 27.206431829668777 28.355018698258444 23.432042990328515 62-63 20.946593967725935 26.976020961143337 28.30844332472048 23.76894174641025 64-65 20.58799060345144 26.923332477655 28.295802834961425 24.192874083932132 66-67 20.86006293841573 26.946653280425988 28.00685499136738 24.186428789790902 68-69 20.858377500995623 26.962528563058243 27.926432286212037 24.2526616497341 70-71 20.781956957502054 26.762847095974617 27.91184458991892 24.543351356604408 72-73 20.906666417213234 26.845694181310247 27.817522395484538 24.430117005991978 74-75 21.005016090116097 26.970591624735622 27.606413486841895 24.417978798306383 76-77 20.926691851869016 26.912116524442077 27.588755362302415 24.572436261386493 78-79 20.85369263123573 26.93749540262662 27.604332634462793 24.60447933167486 80-81 20.834840626118257 26.98613737243139 27.53469425124114 24.644327750209207 82-83 20.993621992695395 26.574397579323737 27.7538420732101 24.678138354770766 84-85 21.131408498531673 26.596106673428377 27.417844380873447 24.854640447166503 86-87 20.830986983360265 26.768175130584588 27.613831542846647 24.787006343208507 88-89 20.98082746224171 26.35491509935099 27.547445383905995 25.11681205450131 90-91 21.03824659801667 26.159178936414172 27.57719145580776 25.225383009761398 92-93 21.052988288768393 26.042328230674656 27.63450012895372 25.270183351603233 94-95 20.646953844869785 25.865285257576264 27.95451739740395 25.533243500150004 96-97 20.80957427588506 25.78232787391115 27.877274688490676 25.530823161713112 98-99 20.7159227264834 25.964899937853758 27.743103191234532 25.576074144428308 100 20.763084016656595 25.69770999697376 27.90482646027196 25.634379526097682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2533.0 1 1758.0 2 1891.0 3 2874.5 4 2186.0 5 1584.5 6 2284.0 7 3787.0 8 5672.5 9 6657.0 10 5748.0 11 4708.5 12 4947.0 13 5479.5 14 6111.0 15 6977.5 16 7962.0 17 8896.0 18 9848.5 19 11054.5 20 12662.0 21 14673.0 22 17226.5 23 20855.0 24 25426.0 25 31037.5 26 38506.5 27 47217.0 28 56781.0 29 68864.0 30 82920.5 31 96695.5 32 114026.0 33 130056.0 34 143658.0 35 158254.0 36 173439.0 37 193189.5 38 206194.0 39 209585.5 40 211736.0 41 215096.5 42 223972.5 43 236415.0 44 248531.5 45 266408.5 46 283239.5 47 295071.0 48 303816.5 49 303294.0 50 295632.0 51 289222.0 52 281585.5 53 267602.5 54 251610.5 55 226497.5 56 198323.0 57 169821.5 58 142225.0 59 121273.0 60 97934.5 61 75748.0 62 58993.5 63 44589.5 64 32107.0 65 22154.5 66 15482.5 67 11710.0 68 8765.0 69 6298.5 70 4753.0 71 3692.5 72 2762.0 73 1943.0 74 1428.0 75 1009.0 76 630.0 77 364.0 78 214.0 79 139.5 80 100.5 81 69.0 82 38.0 83 27.0 84 19.5 85 9.0 86 7.0 87 7.0 88 4.0 89 2.5 90 3.5 91 2.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050507002944590366 2 1.395606602503188E-5 3 0.0 4 0.014249143411557547 5 0.004103083411359372 6 0.0 7 2.232970564005101E-4 8 4.326380467759883E-4 9 0.0021213220358048458 10-11 7.536275653517215E-4 12-13 2.3027508941302597E-4 14-15 3.48901650625797E-5 16-17 1.3258262723780286E-4 18-19 0.0 20-21 2.093409903754782E-5 22-23 6.97803301251594E-6 24-25 5.582426410012752E-5 26-27 2.791213205006376E-5 28-29 0.001144397414052614 30-31 8.582980605394606E-4 32-33 0.013830461430806594 34-35 0.022971684677202474 36-37 0.03102433477364587 38-39 0.02038283442955906 40-41 1.5351672627535068E-4 42-43 0.001863134814341756 44-45 0.01039726918864875 46-47 0.01474458375544618 48-49 0.006161603150051575 50-51 0.011227655117138146 52-53 0.01653096020665026 54-55 0.010215840330323337 56-57 0.0034052801101077785 58-59 0.008017759931380816 60-61 0.0014444528335907994 62-63 0.0011862656121277098 64-65 0.020040910811945777 66-67 0.045531665406666505 68-69 0.05575448377000236 70-71 0.048720626493386286 72-73 0.042112429230533696 74-75 0.02923098028942927 76-77 0.029893893425618285 78-79 0.01292331713917952 80-81 0.0024981358184807062 82-83 0.028686693714453027 84-85 0.04810655958828489 86-87 0.04898579174786189 88-89 0.05955053372881103 90-91 0.035392583439480846 92-93 0.026802624801073725 94-95 0.008115452393556037 96-97 0.00374720372772106 98-99 0.02225992530992585 100 0.018631348143417558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7165343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.86395975824112 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80964624717204 49.82177803221304 2 14.55204153493984 18.878060730451825 3 4.38187733218842 8.526777448218558 4 1.6609685218236012 4.309479814370852 5 0.8185180171334213 2.654615986236878 6 0.48136401754432107 1.8733905757836071 7 0.28504158198666907 1.2942247992386084 8 0.2025804820418688 1.0512137787975093 9 0.14260377765451412 0.8324861125640013 >10 0.6322192889724311 6.962987001466256 >50 0.02354780910176526 1.0403954895511522 >100 0.00861755102559872 0.9503125312108612 >500 4.3567253891341044E-4 0.18481580560431424 >1k 4.6128503723485775E-4 0.6293003689263403 >5k 0.0 0.0 >10k+ 7.688083933658486E-5 0.9901615253661509 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25330 0.3535071524140575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21580 0.30117190482018796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12657 0.1766419276788285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.186819807509564E-5 2 0.0 0.0 0.0 0.0 4.186819807509564E-5 3 0.0 0.0 0.0 0.0 4.186819807509564E-5 4 0.0 0.0 0.0 0.0 4.186819807509564E-5 5 0.0 1.3956066025031879E-5 0.0 0.0 5.5824264100127516E-5 6 2.7912132050063758E-5 1.3956066025031879E-5 0.0 0.0 8.373639615019128E-5 7 2.7912132050063758E-5 1.3956066025031879E-5 0.0 0.0 8.373639615019128E-5 8 2.7912132050063758E-5 1.3956066025031879E-5 0.0 0.0 8.373639615019128E-5 9 2.7912132050063758E-5 1.3956066025031879E-5 0.0 4.186819807509564E-5 8.373639615019128E-5 10-11 2.7912132050063758E-5 1.3956066025031879E-5 0.0 6.97803301251594E-5 8.373639615019128E-5 12-13 2.7912132050063758E-5 1.3956066025031879E-5 0.0 1.2560459422528692E-4 2.0934099037547819E-4 14-15 2.7912132050063758E-5 1.3956066025031879E-5 0.0 1.744508253128985E-4 2.651652544756057E-4 16-17 6.97803301251594E-5 2.7912132050063758E-5 0.0 2.1631902338799412E-4 2.930773865256695E-4 18-19 6.97803301251594E-5 2.7912132050063758E-5 0.0 3.2796755158824917E-4 3.4192361761328105E-4 20-21 6.97803301251594E-5 2.7912132050063758E-5 0.0 7.745616643892693E-4 3.7681378267586074E-4 22-23 6.97803301251594E-5 2.7912132050063758E-5 0.0 0.0022399485970176166 3.907698487008926E-4 24-25 6.97803301251594E-5 4.186819807509564E-5 0.0 0.00568709690520049 4.1868198075095637E-4 26-27 6.97803301251594E-5 4.186819807509564E-5 0.0 0.012309250234078117 4.396160797885042E-4 28-29 6.97803301251594E-5 4.186819807509564E-5 0.0 0.03646022249039578 4.60550178826052E-4 30-31 6.97803301251594E-5 5.582426410012752E-5 0.0 0.11533990766387596 4.6752821183856794E-4 32-33 6.97803301251594E-5 8.373639615019128E-5 0.0 0.28164039041815586 4.745062448510839E-4 34-35 6.97803301251594E-5 9.769246217522315E-5 0.0 0.5476848770533386 5.024183769011477E-4 36-37 6.97803301251594E-5 1.5351672627535068E-4 0.0 0.9539320029759915 5.024183769011477E-4 38-39 6.97803301251594E-5 1.6049475928786662E-4 0.0 1.4833344335365384 5.442865749762433E-4 40-41 6.97803301251594E-5 1.744508253128985E-4 0.0 2.011515708319895 5.442865749762433E-4 42-43 6.97803301251594E-5 1.8142885832541443E-4 0.0 2.529550923103053 5.582426410012752E-4 44-45 6.97803301251594E-5 2.0236295736296225E-4 0.0 3.162123571753648 5.79176740038823E-4 46-47 6.97803301251594E-5 2.2329705640051006E-4 0.0 3.905842330227597 6.001108390763708E-4 48-49 6.97803301251594E-5 2.30275089413026E-4 0.0 4.642827286844469 6.419790371514665E-4 50-51 6.97803301251594E-5 2.581872214630898E-4 0.0 5.355912480393472 6.838472352265621E-4 52-53 6.97803301251594E-5 2.7214328748812166E-4 0.0 6.017297427352744 7.257154333016577E-4 54-55 6.97803301251594E-5 2.930773865256695E-4 0.0 6.740961318948723 7.396714993266896E-4 56-57 7.675836313767534E-5 2.930773865256695E-4 0.0 7.574836263944378 7.675836313767534E-4 58-59 8.373639615019128E-5 2.930773865256695E-4 0.0 8.453335730055073 7.815396974017852E-4 60-61 8.373639615019128E-5 3.000554195381854E-4 0.0 9.311103460085581 7.815396974017852E-4 62-63 9.769246217522315E-5 3.2796755158824917E-4 0.0 10.134155475878824 8.792321595770084E-4 64-65 1.0467049518773909E-4 3.349455846007651E-4 0.0 10.957982053336455 9.071442916270721E-4 66-67 1.1164852820025503E-4 3.558796836383129E-4 0.0 11.86407266197864 9.21100357652104E-4 68-69 1.1164852820025503E-4 3.6285771665082886E-4 0.0 12.828743857760891 9.21100357652104E-4 70-71 1.1164852820025503E-4 3.6285771665082886E-4 0.0 13.798857919292907 9.420344566896518E-4 72-73 1.1164852820025503E-4 3.6285771665082886E-4 0.0 14.732679231126827 9.839026547647475E-4 74-75 1.1164852820025503E-4 3.6285771665082886E-4 0.0 15.618868489617315 0.0010048367538022954 76-77 1.1862656121277098E-4 3.6285771665082886E-4 0.0 16.558509480983673 0.0010048367538022954 78-79 1.2560459422528692E-4 3.6285771665082886E-4 0.0 17.549878631071813 0.0010118147868148114 80-81 1.2560459422528692E-4 3.6285771665082886E-4 0.0 18.553452360898845 0.001025770852839843 82-83 1.3258262723780286E-4 3.6285771665082886E-4 0.0 19.53152138006513 0.001046704951877391 84-85 1.395606602503188E-4 3.6285771665082886E-4 0.0 20.458259988391344 0.001046704951877391 86-87 1.395606602503188E-4 3.6285771665082886E-4 0.0 21.388396898794657 0.0011095072489900343 88 1.395606602503188E-4 3.6285771665082886E-4 0.0 22.10555726362297 0.001172309546102678 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11665 0.0 56.112812 1 GTATCAA 21390 0.0 40.93327 1 ATCAACG 25815 0.0 33.35196 3 CAACGCA 25915 0.0 33.23583 5 TATCAAC 26880 0.0 32.309826 2 TCAACGC 27060 0.0 31.83484 4 AACGCAG 27625 0.0 31.397886 6 TACCTGG 4935 0.0 29.140446 2 GTGGTAT 4390 0.0 27.849432 1 ACGCAGA 30980 0.0 27.618465 7 CGCAGAG 31390 0.0 27.182882 8 GTACTAG 2865 0.0 24.619215 1 GCAGAGT 34470 0.0 24.48139 9 TGGTATC 5035 0.0 23.801407 2 GTACATG 19995 0.0 23.352608 1 TACATGG 20245 0.0 22.424421 2 TAACGCA 610 0.0 22.342728 4 ACATGGG 19375 0.0 22.267372 3 GAGTACT 24025 0.0 21.542152 12-13 CAGAGTA 33920 0.0 21.4221 10-11 >>END_MODULE