FastQCFastQC Report
Mon 6 Jun 2016
ERR1211384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107837
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216780.4244066519742114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174330.3412990665128899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108570.2125557256427721No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.11680090809475714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82900.052.220431
GTATCAA156300.042.917111
TACCTGG49200.042.6623152
ACCTGGG59700.035.1179123
ATCAACG191600.034.2743383
CAACGCA194250.033.806765
TATCAAC195900.033.7533262
TCAACGC200300.032.855724
AACGCAG206250.032.2959676
GTACATG148650.028.8056981
ACGCAGA227250.028.670837
CGCAGAG230050.028.2583548
TACATGG151550.027.9176242
TATCACG5100.027.6838042
CCTGGGG57200.027.1195094
ACATGGG151800.026.383723
GTGGTAT35400.026.214551
GTACCTG83700.025.7762361
CATGGGG114900.025.6923314
GCAGAGT250750.025.1942239
Produced by FastQC (version 0.11.5)