##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211384_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5107837 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36507938683243 31.0 31.0 34.0 28.0 34.0 2 31.409858223745196 31.0 31.0 34.0 28.0 34.0 3 31.474389061358067 31.0 31.0 34.0 28.0 34.0 4 35.102407535714235 37.0 35.0 37.0 32.0 37.0 5 35.161545288152304 37.0 35.0 37.0 32.0 37.0 6 35.224653018489036 37.0 35.0 37.0 32.0 37.0 7 35.1568632671716 37.0 35.0 37.0 32.0 37.0 8 35.15862663589304 37.0 35.0 37.0 32.0 37.0 9 36.73232074555237 39.0 37.0 39.0 32.0 39.0 10-11 36.663162117350254 39.0 37.0 39.0 32.0 39.0 12-13 36.576105110636846 39.0 37.0 39.0 32.0 39.0 14-15 37.764399294652506 40.0 37.0 41.0 32.0 41.0 16-17 37.74974681063628 40.0 37.0 41.0 32.0 41.0 18-19 37.75677228932717 40.0 37.0 41.0 32.0 41.0 20-21 37.736771944758615 40.0 37.0 41.0 32.0 41.0 22-23 37.61174563714543 40.0 37.0 41.0 32.0 41.0 24-25 37.51595166408012 40.0 37.0 41.0 32.0 41.0 26-27 37.37600318882533 40.0 36.5 41.0 31.0 41.0 28-29 37.23550252288787 40.0 36.0 41.0 31.0 41.0 30-31 37.064568426909474 39.0 36.0 41.0 30.5 41.0 32-33 36.84382675093195 39.0 35.5 41.0 30.0 41.0 34-35 36.70357599508364 39.0 35.0 41.0 30.0 41.0 36-37 36.55990471113311 39.0 35.0 41.0 30.0 41.0 38-39 36.396740733895776 39.0 35.0 41.0 29.5 41.0 40-41 36.223530136141775 39.0 35.0 40.0 29.0 41.0 42-43 36.01715021446456 39.0 35.0 40.0 28.0 41.0 44-45 35.877054122909556 38.5 35.0 40.0 27.5 41.0 46-47 35.64449736747668 38.0 34.5 40.0 26.5 41.0 48-49 35.388020212861136 38.0 34.0 40.0 26.0 41.0 50-51 34.713747913255645 37.5 33.5 39.5 25.0 40.5 52-53 35.009783397551644 38.0 34.0 39.5 26.0 40.5 54-55 35.415573069383385 38.0 34.0 40.0 26.0 41.0 56-57 35.33930193935319 38.0 34.0 40.0 26.0 41.0 58-59 35.1412343424428 38.0 34.0 40.0 26.0 41.0 60-61 34.91275338269408 38.0 34.0 40.0 26.0 41.0 62-63 34.63105165650352 37.0 33.5 40.0 25.0 41.0 64-65 34.41666355054008 37.0 33.0 40.0 25.0 41.0 66-67 34.209179051719936 36.5 33.0 39.5 24.5 41.0 68-69 33.869480956420496 36.0 33.0 39.0 23.5 41.0 70-71 33.491584892000276 35.5 32.5 39.0 23.5 41.0 72-73 33.098411127841395 35.0 32.0 38.5 22.5 40.0 74-75 32.716472060482744 35.0 32.0 37.0 22.0 40.0 76-77 32.32018895669537 35.0 31.0 37.0 20.5 39.0 78-79 31.90997275363329 35.0 31.0 36.0 20.0 39.0 80-81 31.498442099855573 35.0 31.0 36.0 19.5 38.5 82-83 31.119061062441894 35.0 30.0 35.5 18.0 37.0 84-85 30.774523051538253 34.0 30.0 35.0 17.5 37.0 86-87 30.429050692103132 34.0 29.5 35.0 16.0 36.0 88-89 30.100341103288926 34.0 29.0 35.0 14.0 36.0 90-91 29.786983120252273 34.0 29.0 35.0 11.0 36.0 92-93 29.485354368199296 34.0 28.5 35.0 10.0 35.0 94-95 29.19276740820038 34.0 27.0 35.0 10.0 35.0 96-97 28.892227473194623 34.0 27.0 35.0 10.0 35.0 98-99 28.567083973118173 34.0 25.5 35.0 9.0 35.0 100 27.29894552234145 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3341877719318056 1101 2 -0.2461283092147255 1101 3 -0.43115597180201704 1101 4 -0.3219861320536026 1101 5 -0.2914083509002836 1101 6 -0.23795209087685265 1101 7 -0.3391602266231004 1101 8 -0.28268219157551755 1101 9 -0.4434554339358243 1101 10-11 -0.4262367831381937 1101 12-13 -0.476794305893641 1101 14-15 -0.6658560312626136 1101 16-17 -0.6480450069335433 1101 18-19 -0.6363197962947282 1101 20-21 -0.6685150097057715 1101 22-23 -0.6855680848372288 1101 24-25 -0.6728744242916918 1101 26-27 -0.7207032518304999 1101 28-29 -0.7473737314755837 1101 30-31 -0.6875292003715998 1101 32-33 -0.666987418031951 1101 34-35 -0.7723926944951813 1101 36-37 -0.8569020923266848 1101 38-39 -0.9136186413210581 1101 40-41 -1.079401762013184 1101 42-43 -1.095639338104938 1101 44-45 -1.0250931305709017 1101 46-47 -1.0213861019143948 1101 48-49 -1.077520975122276 1101 50-51 -0.9817701758805057 1101 52-53 -0.7732417685792043 1101 54-55 -0.7198218726601766 1101 56-57 -0.7772354876974319 1101 58-59 -0.744839300235661 1101 60-61 -0.7838486005288132 1101 62-63 -0.9927261375593375 1101 64-65 -0.8815822094143044 1101 66-67 -0.9371171031048036 1101 68-69 -0.9313248711052964 1101 70-71 -0.8815989886379114 1101 72-73 -1.1209227695994173 1101 74-75 -1.2802663171403168 1101 76-77 -1.1065390135314495 1101 78-79 -1.1463140863092818 1101 80-81 -1.2685430567874043 1101 82-83 -1.3587307178571635 1101 84-85 -1.348911468379459 1101 86-87 -1.500333118938908 1101 88-89 -1.4751862852888173 1101 90-91 -1.5773444227424314 1101 92-93 -1.7309438846603342 1101 94-95 -1.8307109619548037 1101 96-97 -1.9921836853386665 1101 98-99 -2.0238894454702923 1101 100 -2.00982384413809 1102 1 -0.3596677529594068 1102 2 -0.4034772223526666 1102 3 -0.5078926347812427 1102 4 -0.3687762727410018 1102 5 -0.36267931002272036 1102 6 -0.3956538825016054 1102 7 -0.47375570432832603 1102 8 -0.4477874346151651 1102 9 -0.5457928649436141 1102 10-11 -0.5305000855431814 1102 12-13 -0.5511857032813978 1102 14-15 -0.7807971070918072 1102 16-17 -0.753302822736309 1102 18-19 -0.8032608728604345 1102 20-21 -0.9109258666895457 1102 22-23 -0.7906517169465346 1102 24-25 -0.8615202896937291 1102 26-27 -0.8229152006109217 1102 28-29 -0.8581015320768657 1102 30-31 -0.7640462464371112 1102 32-33 -0.8295851339792222 1102 34-35 -0.828080795576227 1102 36-37 -0.8317153327915037 1102 38-39 -1.0989912434413114 1102 40-41 -1.1187545259732872 1102 42-43 -1.0913508932228453 1102 44-45 -1.0234035855696462 1102 46-47 -1.3282025303458909 1102 48-49 -1.253658204990252 1102 50-51 -1.1666686982283139 1102 52-53 -0.9001971002205451 1102 54-55 -0.9273035134042829 1102 56-57 -0.9353767893394007 1102 58-59 -0.9024829539098747 1102 60-61 -1.012461061696527 1102 62-63 -0.9338110810177582 1102 64-65 -1.079638229173291 1102 66-67 -1.1323240410659565 1102 68-69 -1.043814716527386 1102 70-71 -1.0875739680525598 1102 72-73 -0.9806902122734655 1102 74-75 -1.1642893454766536 1102 76-77 -1.2544080343907744 1102 78-79 -1.3360144903565292 1102 80-81 -1.254961055670865 1102 82-83 -1.2890425103509884 1102 84-85 -1.4572611828360422 1102 86-87 -1.540120881816346 1102 88-89 -1.5609619956121286 1102 90-91 -1.5851609159864637 1102 92-93 -1.572840467051062 1102 94-95 -1.7218055076115562 1102 96-97 -1.7892437555129668 1102 98-99 -1.8805026626615238 1102 100 -1.923503855804345 1103 1 -0.41241755865669916 1103 2 -0.39061349144032675 1103 3 -0.4863978274506806 1103 4 -0.2909942240583163 1103 5 -0.2270049576045423 1103 6 -0.32312002502028747 1103 7 -0.3705030113115839 1103 8 -0.3067656745893359 1103 9 -0.4059683662821385 1103 10-11 -0.4666149512959734 1103 12-13 -0.5767821985111254 1103 14-15 -0.702255025067906 1103 16-17 -0.7598735160684456 1103 18-19 -0.6741262614470713 1103 20-21 -0.6142304206944331 1103 22-23 -0.734658440571117 1103 24-25 -0.7066392789817328 1103 26-27 -0.6789597982447475 1103 28-29 -0.7643676535695718 1103 30-31 -0.7831843355873218 1103 32-33 -0.8868567485126917 1103 34-35 -0.8483823543157101 1103 36-37 -0.8065301820238702 1103 38-39 -0.8445688856765301 1103 40-41 -0.8669817953725811 1103 42-43 -0.8015749467715523 1103 44-45 -0.9211915384263634 1103 46-47 -0.9416092528440743 1103 48-49 -0.9607194891903461 1103 50-51 -0.9535214684029327 1103 52-53 -0.7735032405615385 1103 54-55 -0.8451183729619061 1103 56-57 -0.7889715771458228 1103 58-59 -0.7515276557350035 1103 60-61 -0.768219156703978 1103 62-63 -0.8340953543256404 1103 64-65 -0.7307110476326102 1103 66-67 -0.6854014157829482 1103 68-69 -0.6236409564039391 1103 70-71 -0.7933300214736008 1103 72-73 -0.9194682219003667 1103 74-75 -0.8838225402637079 1103 76-77 -0.9179997668575375 1103 78-79 -0.9092339454078378 1103 80-81 -0.8760394732395831 1103 82-83 -0.9551315253958386 1103 84-85 -1.0382568293290184 1103 86-87 -1.041354070408243 1103 88-89 -1.1312496387109086 1103 90-91 -1.1396868783949898 1103 92-93 -1.2980009075831695 1103 94-95 -1.3927524423220916 1103 96-97 -1.4648420107309796 1103 98-99 -1.383089845441237 1103 100 -1.443106089065214 1104 1 -0.4763702275084931 1104 2 -0.4436282740506634 1104 3 -0.4436561476223204 1104 4 -0.25956381686665253 1104 5 -0.3566978325524133 1104 6 -0.38909869835931943 1104 7 -0.3258173163269902 1104 8 -0.44315643630701373 1104 9 -0.5153962902066098 1104 10-11 -0.5057567900081423 1104 12-13 -0.5669942857575876 1104 14-15 -0.7126379737401294 1104 16-17 -0.7049166288702864 1104 18-19 -0.7030864923758244 1104 20-21 -0.706658484255513 1104 22-23 -0.7326464233316585 1104 24-25 -0.7336438898201791 1104 26-27 -0.6268483842731598 1104 28-29 -0.7708981179367598 1104 30-31 -0.8794617227600057 1104 32-33 -0.865441069208913 1104 34-35 -0.8232033741535503 1104 36-37 -0.8838132405183998 1104 38-39 -0.9283921736657064 1104 40-41 -0.9689440906587166 1104 42-43 -1.0600708907055605 1104 44-45 -1.05853836273144 1104 46-47 -0.9854078544023466 1104 48-49 -0.9826847989288652 1104 50-51 -1.0926197414377938 1104 52-53 -0.8964153195052873 1104 54-55 -0.8199235893326033 1104 56-57 -0.8176593515836501 1104 58-59 -0.905244466095283 1104 60-61 -0.7960025954252075 1104 62-63 -0.8927054370320207 1104 64-65 -0.7738523900687397 1104 66-67 -0.8791351759514185 1104 68-69 -0.8628038585432094 1104 70-71 -0.8584560859668429 1104 72-73 -0.9029041126592503 1104 74-75 -0.8553925746256326 1104 76-77 -0.8731925738740856 1104 78-79 -0.781369295735324 1104 80-81 -0.8644940262420526 1104 82-83 -0.8777775164100348 1104 84-85 -0.8857710382970829 1104 86-87 -1.0127598666482456 1104 88-89 -1.1190680903673957 1104 90-91 -0.9945999640431751 1104 92-93 -1.0191694395307032 1104 94-95 -1.1000971862304425 1104 96-97 -1.1573515918777737 1104 98-99 -1.2213835804751163 1104 100 -1.1922056688345108 1105 1 -0.5253190875706153 1105 2 -0.5028652242496605 1105 3 -0.47517627939783225 1105 4 -0.35380612796326716 1105 5 -0.4428936318666743 1105 6 -0.44508779503885165 1105 7 -0.46615190939132134 1105 8 -0.49675531240018245 1105 9 -0.6334459860596056 1105 10-11 -0.6202442376489969 1105 12-13 -0.6945500282087025 1105 14-15 -0.8386018884055559 1105 16-17 -0.873281535726079 1105 18-19 -0.7810713418445516 1105 20-21 -0.8773127259135549 1105 22-23 -0.7757028848735814 1105 24-25 -0.8830732613776107 1105 26-27 -0.842316598540819 1105 28-29 -0.8588727353963108 1105 30-31 -0.917274660085468 1105 32-33 -0.9963149969832727 1105 34-35 -1.077026057576127 1105 36-37 -1.0065056194471609 1105 38-39 -1.028848126678703 1105 40-41 -0.9933429774146347 1105 42-43 -1.0139130272132775 1105 44-45 -0.9082925888196414 1105 46-47 -1.051843991095275 1105 48-49 -0.9927375888277936 1105 50-51 -1.025812160582504 1105 52-53 -0.8540098306914032 1105 54-55 -0.855644028667939 1105 56-57 -0.8680654405007573 1105 58-59 -0.6689767161927804 1105 60-61 -0.7547763518943498 1105 62-63 -0.8943182490607953 1105 64-65 -0.8117874280174817 1105 66-67 -0.7703148114030043 1105 68-69 -0.7746156860558671 1105 70-71 -0.809116895923573 1105 72-73 -0.7748441488240942 1105 74-75 -0.7972472662596459 1105 76-77 -0.9519006187624726 1105 78-79 -0.8810847355632134 1105 80-81 -0.8094674837110958 1105 82-83 -0.8092936634853203 1105 84-85 -0.8529352405988391 1105 86-87 -0.9167085062605196 1105 88-89 -0.8856040889978409 1105 90-91 -0.992549826552569 1105 92-93 -0.941503573616604 1105 94-95 -1.0064387905224095 1105 96-97 -1.019054315162112 1105 98-99 -1.0094898590713335 1105 100 -0.9860153724650544 1106 1 -0.4520023987527999 1106 2 -0.4158608776399042 1106 3 -0.5052471179212894 1106 4 -0.40631374089664973 1106 5 -0.36722921932542363 1106 6 -0.40023316454583124 1106 7 -0.42431224613935115 1106 8 -0.4764181562102934 1106 9 -0.6112358703625276 1106 10-11 -0.7529900056779795 1106 12-13 -0.6535369016892503 1106 14-15 -0.7675200906172819 1106 16-17 -0.7545899398518827 1106 18-19 -0.8901819791095207 1106 20-21 -0.8531088488395113 1106 22-23 -0.7458433311004669 1106 24-25 -0.7726207322907968 1106 26-27 -0.8087869119384834 1106 28-29 -0.7607609830788391 1106 30-31 -0.8472516113325312 1106 32-33 -0.9110149703802222 1106 34-35 -0.831499406575098 1106 36-37 -0.8507221830879601 1106 38-39 -0.8552467418752414 1106 40-41 -0.9320627241203638 1106 42-43 -0.8446887291825362 1106 44-45 -0.9430788090174005 1106 46-47 -0.9215447149041438 1106 48-49 -0.8441311741875595 1106 50-51 -0.8598509804473409 1106 52-53 -0.8110782323176409 1106 54-55 -0.757491008210323 1106 56-57 -0.9038433602939122 1106 58-59 -0.8098886996479635 1106 60-61 -0.6666096569630753 1106 62-63 -0.7615859812220833 1106 64-65 -0.7135599307576967 1106 66-67 -0.7117770382318369 1106 68-69 -0.6391288150134784 1106 70-71 -0.7101603883893901 1106 72-73 -0.6937322289056027 1106 74-75 -0.6459992617141914 1106 76-77 -0.6162464492083899 1106 78-79 -0.6502440791668072 1106 80-81 -0.6703552020416801 1106 82-83 -0.6703977686448468 1106 84-85 -0.7095936292558633 1106 86-87 -0.7554729571079974 1106 88-89 -0.7457111667276912 1106 90-91 -0.8514164896432703 1106 92-93 -0.7999819369203607 1106 94-95 -0.9067972724978048 1106 96-97 -0.9177686635977764 1106 98-99 -0.8756921598288443 1106 100 -0.8733961079915389 1107 1 -0.5646761115441272 1107 2 -0.6112389944443137 1107 3 -0.5758818987311543 1107 4 -0.47291041189637895 1107 5 -0.39784809106722463 1107 6 -0.43776562946731445 1107 7 -0.5578931302856702 1107 8 -0.4976607565360922 1107 9 -0.6458609755808808 1107 10-11 -0.7318793416291456 1107 12-13 -0.8048624545518521 1107 14-15 -0.8465551450609965 1107 16-17 -0.9114466317921099 1107 18-19 -0.9898819579997138 1107 20-21 -0.9999506184230924 1107 22-23 -0.897108504102988 1107 24-25 -0.8459364979285837 1107 26-27 -0.9946907475211475 1107 28-29 -0.9945077564267351 1107 30-31 -1.0611062298561507 1107 32-33 -1.0799969699072278 1107 34-35 -1.0342360625598843 1107 36-37 -1.0344399106148714 1107 38-39 -1.0184865042814906 1107 40-41 -1.0361323399166409 1107 42-43 -0.9988236319425212 1107 44-45 -1.0057872947883908 1107 46-47 -1.1133491111399252 1107 48-49 -1.0331106791599964 1107 50-51 -1.189148187974702 1107 52-53 -0.8719300208577181 1107 54-55 -0.8405251537156033 1107 56-57 -0.7393876017350536 1107 58-59 -0.7612292119019628 1107 60-61 -0.8179601421446847 1107 62-63 -0.7938704244602519 1107 64-65 -0.7132850139139251 1107 66-67 -0.8374474332391415 1107 68-69 -0.7970014493530471 1107 70-71 -0.6318112041854462 1107 72-73 -0.7266249678207046 1107 74-75 -0.728567695308513 1107 76-77 -0.7987106167705065 1107 78-79 -0.7544285026707449 1107 80-81 -0.5530172277258067 1107 82-83 -0.6196734616310522 1107 84-85 -0.7135089292081922 1107 86-87 -0.7153174402802058 1107 88-89 -0.7517613874283136 1107 90-91 -0.7850573700755348 1107 92-93 -0.8192696318090888 1107 94-95 -0.8130221252450127 1107 96-97 -0.866097259129166 1107 98-99 -0.755831656484343 1107 100 -0.7849961570890294 1108 1 -0.41059934002502274 1108 2 -0.468785419062538 1108 3 -0.44138780497603847 1108 4 -0.37077109745117554 1108 5 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2311 38-39 1.2169317598008647 2311 40-41 1.2332009751980522 2311 42-43 1.2274241916744089 2311 44-45 1.2245235835445456 2311 46-47 1.3169028368970075 2311 48-49 1.3214168895851515 2311 50-51 1.3286733565841473 2311 52-53 1.138997642554294 2311 54-55 1.1327382200368419 2311 56-57 1.1385069505234569 2311 58-59 1.076761962217617 2311 60-61 1.0866881688909658 2311 62-63 1.1340422851263767 2311 64-65 1.1442810387319895 2311 66-67 1.1762919095128836 2311 68-69 1.1656815538287333 2311 70-71 1.1610548665564906 2311 72-73 1.117052769648332 2311 74-75 1.1300548107741477 2311 76-77 1.1177458260503812 2311 78-79 1.1593267638741018 2311 80-81 1.237131935644424 2311 82-83 1.28278199072642 2311 84-85 1.2347352405201661 2311 86-87 1.2942300384613574 2311 88-89 1.2939651763485749 2311 90-91 1.3541753518647361 2311 92-93 1.3887113416234023 2311 94-95 1.3822469842260716 2311 96-97 1.4149362423236838 2311 98-99 1.5598452737587571 2311 100 1.5021036612676184 2312 1 0.5719040122939205 2312 2 0.6359687057690948 2312 3 0.7051138985134848 2312 4 0.5433518687167336 2312 5 0.6296938167358661 2312 6 0.4528192363899137 2312 7 0.5571073760145424 2312 8 0.616494233549588 2312 9 0.7769293742030428 2312 10-11 0.7807601883759148 2312 12-13 0.9057064672264588 2312 14-15 1.057629212497588 2312 16-17 1.0508562523473373 2312 18-19 1.0409756754263597 2312 20-21 1.023834572661201 2312 22-23 0.9891972750641997 2312 24-25 1.103203765155932 2312 26-27 1.1240659089360747 2312 28-29 1.1424011792725182 2312 30-31 1.1001881948976973 2312 32-33 1.1637779324149449 2312 34-35 1.2329730200642928 2312 36-37 1.2505080671664288 2312 38-39 1.248351995425807 2312 40-41 1.3180474284914467 2312 42-43 1.3624648902675744 2312 44-45 1.4388650263277114 2312 46-47 1.398875335094857 2312 48-49 1.479045522131777 2312 50-51 1.4063066576171153 2312 52-53 1.227769196638043 2312 54-55 1.22613341626802 2312 56-57 1.1894681010544588 2312 58-59 1.200298100651331 2312 60-61 1.2422389023437148 2312 62-63 1.161580873424647 2312 64-65 1.1828264561913358 2312 66-67 1.1839814273566915 2312 68-69 1.1573166734443774 2312 70-71 1.2104330129956153 2312 72-73 1.2396748037312832 2312 74-75 1.1673649472932865 2312 76-77 1.1674003866938634 2312 78-79 1.2039230250219184 2312 80-81 1.004503524660013 2312 82-83 1.1685281775043137 2312 84-85 1.2308142710129921 2312 86-87 1.2067851034895263 2312 88-89 1.3130758924199348 2312 90-91 1.3395216358845907 2312 92-93 1.4353868107865075 2312 94-95 1.4495820461537292 2312 96-97 1.4056074913060286 2312 98-99 1.2853686139407507 2312 100 1.3814106637380696 2313 1 0.49997615906283954 2313 2 0.5086375395921579 2313 3 0.5446816729044208 2313 4 0.4557654718979549 2313 5 0.4350579209018264 2313 6 0.48468584550680305 2313 7 0.568939523881312 2313 8 0.4833678191064692 2313 9 0.6597588093667994 2313 10-11 0.6324052471928994 2313 12-13 0.705645138159916 2313 14-15 0.9350398036821232 2313 16-17 0.9350858766063297 2313 18-19 0.9301202291580211 2313 20-21 0.9234814567558018 2313 22-23 0.9838993928937754 2313 24-25 0.9991362436071611 2313 26-27 0.9756801044530476 2313 28-29 1.005022994689135 2313 30-31 1.0028028024336066 2313 32-33 1.0012597180306955 2313 34-35 0.9899380650013612 2313 36-37 0.9655294789722149 2313 38-39 1.0041409403360504 2313 40-41 1.0543100606617841 2313 42-43 1.0768345514067903 2313 44-45 1.0390900193763173 2313 46-47 1.0016790133576308 2313 48-49 1.046384300802245 2313 50-51 0.9784327330132072 2313 52-53 0.8498729611492379 2313 54-55 0.7932086792636355 2313 56-57 0.7975171516737518 2313 58-59 0.7627241008863663 2313 60-61 0.7807186984406229 2313 62-63 0.8181792019107945 2313 64-65 0.7933238092174975 2313 66-67 0.8118611352556329 2313 68-69 0.7917061279380704 2313 70-71 0.7950651371628439 2313 72-73 0.8772143161069366 2313 74-75 0.8541288364862254 2313 76-77 0.7534103455061327 2313 78-79 0.7798763946734759 2313 80-81 0.8467912175576764 2313 82-83 0.8552213819801757 2313 84-85 0.8506451787931795 2313 86-87 0.8501946330963506 2313 88-89 0.8723392961319405 2313 90-91 0.9256847403791468 2313 92-93 1.0929564959519844 2313 94-95 1.1324981790091968 2313 96-97 1.0834044278369248 2313 98-99 1.1787014686002273 2313 100 1.3255553606906858 2314 1 0.4182718276036148 2314 2 0.4459111217196785 2314 3 0.47489303234854674 2314 4 0.3550598718898499 2314 5 0.40913501738276636 2314 6 0.38504048535389046 2314 7 0.47070195343525256 2314 8 0.39505807401550896 2314 9 0.6043729880861903 2314 10-11 0.5557236872258144 2314 12-13 0.37307360220723496 2314 14-15 0.7723841564630547 2314 16-17 0.772403103150971 2314 18-19 0.81500426732633 2314 20-21 0.7630586328106261 2314 22-23 0.6955378978400191 2314 24-25 0.6893974425943483 2314 26-27 0.8647858281373999 2314 28-29 0.8854055187986134 2314 30-31 0.8721483155817253 2314 32-33 0.8616715540992317 2314 34-35 0.9029208837227927 2314 36-37 1.0036668929965913 2314 38-39 1.0601297210109237 2314 40-41 1.1055215393859328 2314 42-43 1.1930044092717722 2314 44-45 1.2442999161048292 2314 46-47 1.2026644903963302 2314 48-49 1.2588872895370216 2314 50-51 1.1817686836108052 2314 52-53 1.1129663670979184 2314 54-55 1.069371853353303 2314 56-57 1.069933308975969 2314 58-59 1.0778875565285873 2314 60-61 1.0294406675026053 2314 62-63 1.015876222162433 2314 64-65 1.099944870943233 2314 66-67 1.0713902022434851 2314 68-69 1.0448842076117373 2314 70-71 1.0351605859568664 2314 72-73 0.9865851054119901 2314 74-75 1.0974475663684942 2314 76-77 1.0737470060730345 2314 78-79 1.166175953660673 2314 80-81 1.189809236813371 2314 82-83 1.00665942808088 2314 84-85 1.0787892712053413 2314 86-87 1.2255497524340093 2314 88-89 1.3879315079335939 2314 90-91 1.433620161188152 2314 92-93 1.514102224127381 2314 94-95 1.6303652406252453 2314 96-97 1.6080967241905455 2314 98-99 1.639879554846683 2314 100 1.566918523294131 2315 1 0.45894320716927695 2315 2 0.49431962596195334 2315 3 0.5315194695336665 2315 4 0.42267319765556266 2315 5 0.443729181290351 2315 6 -0.25636762625575926 2315 7 0.23219950631285258 2315 8 0.31745328732302625 2315 9 0.5386444296781363 2315 10-11 0.647511990905123 2315 12-13 0.7175770919045803 2315 14-15 0.7810520283536846 2315 16-17 0.6770340631292484 2315 18-19 0.792213218095057 2315 20-21 0.8336611833406664 2315 22-23 0.7590085898344299 2315 24-25 0.8389981222684924 2315 26-27 0.8606971666433338 2315 28-29 0.9326089559749775 2315 30-31 0.9808525612308898 2315 32-33 0.9976947543336365 2315 34-35 1.0218404157381684 2315 36-37 1.0672203215632763 2315 38-39 1.0927557926280045 2315 40-41 1.1950243890777088 2315 42-43 1.2687223497325562 2315 44-45 1.2233614051460862 2315 46-47 1.2886044637579772 2315 48-49 1.2870115005618246 2315 50-51 1.2354371445157497 2315 52-53 1.015223788972456 2315 54-55 0.8749870490596834 2315 56-57 0.8505967208319092 2315 58-59 0.8622495991125518 2315 60-61 0.735314179653102 2315 62-63 0.9023622202919697 2315 64-65 0.9127447207413155 2315 66-67 0.8933214605467228 2315 68-69 0.9274850777839632 2315 70-71 0.9186144874477833 2315 72-73 0.9481923679257918 2315 74-75 0.9694904628231811 2315 76-77 1.0098711689830182 2315 78-79 0.9881724807642875 2315 80-81 0.919049162543967 2315 82-83 1.0809488813513823 2315 84-85 1.0264880687662874 2315 86-87 1.13766255397044 2315 88-89 1.3039815400253936 2315 90-91 1.3322145154630718 2315 92-93 1.4179433950120028 2315 94-95 1.4156710920281128 2315 96-97 1.4901947944855145 2315 98-99 1.507117218919202 2315 100 1.5401663691426108 2316 1 0.039582435033921115 2316 2 -0.034396327585977104 2316 3 0.10911673117421472 2316 4 0.058557678718386796 2316 5 0.040702721240450046 2316 6 0.17440945072479508 2316 7 0.15088852883016557 2316 8 0.1953888900785401 2316 9 0.29834092078803565 2316 10-11 0.21725004345585575 2316 12-13 0.2948422715669139 2316 14-15 0.5389457335295376 2316 16-17 0.5841279575216376 2316 18-19 0.4832782746095319 2316 20-21 0.4069662999016401 2316 22-23 0.29950680166602694 2316 24-25 0.36896882667509345 2316 26-27 0.43917591049897453 2316 28-29 0.570892721281048 2316 30-31 0.5288761615249911 2316 32-33 0.6398719989199577 2316 34-35 0.6773056788814174 2316 36-37 0.749112793457364 2316 38-39 0.7632101557292899 2316 40-41 0.8150214423894582 2316 42-43 0.8123809748680912 2316 44-45 0.6736896814145936 2316 46-47 0.6301490461338233 2316 48-49 0.7514900494311902 2316 50-51 0.4334260426616936 2316 52-53 0.25462978143908543 2316 54-55 0.2667287620463199 2316 56-57 0.4150743810584103 2316 58-59 0.4141348034534005 2316 60-61 0.4444320032662432 2316 62-63 0.45255731603937477 2316 64-65 0.48736757877392023 2316 66-67 0.45569912887403774 2316 68-69 0.5245974392055714 2316 70-71 0.40000555869904275 2316 72-73 0.5022650761705592 2316 74-75 0.5772165300285366 2316 76-77 0.7050379949399854 2316 78-79 0.7394243918294272 2316 80-81 0.7939035828789933 2316 82-83 0.8343753144071471 2316 84-85 0.9624601262282546 2316 86-87 1.076136154000526 2316 88-89 1.066864768874506 2316 90-91 1.121430550856303 2316 92-93 1.2158879769242041 2316 94-95 1.257174938499471 2316 96-97 1.3091479499984047 2316 98-99 1.4259481795975297 2316 100 1.3375545833426337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19.0 1 299.0 2 151.0 3 114.0 4 100.0 5 74.0 6 97.0 7 585.0 8 2038.0 9 3293.0 10 4427.0 11 5778.0 12 7407.0 13 8852.0 14 10846.0 15 13614.0 16 17645.0 17 23743.0 18 29822.0 19 36253.0 20 43600.0 21 51868.0 22 57134.0 23 57680.0 24 57514.0 25 59724.0 26 65343.0 27 74999.0 28 88352.0 29 105868.0 30 127676.0 31 156998.0 32 196530.0 33 251001.0 34 341660.0 35 463159.0 36 656736.0 37 947562.0 38 898274.0 39 240619.0 40 383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63161526780706 18.53806444447889 12.217663710556115 23.61265657715794 2 16.40676223401085 20.746546023221015 39.80782744546889 23.03886429729925 3 18.893301267596616 26.669816842658108 30.37778808689081 24.059093802854466 4 12.688237654365109 16.89148820467771 38.08312035910871 32.337153781848464 5 12.761869101482699 37.26180721796784 34.63451909458934 15.34180458596012 6 29.453649418628224 36.8432228664605 19.20668909083394 14.496438624077335 7 26.111156495288828 33.34739916337697 23.00685914912699 17.534585192207217 8 25.722031154534854 34.748936275242464 21.20070386737236 18.328328702850317 9 27.181551273990767 15.747755899882678 20.846696085286574 36.22399674083997 10-11 24.62838423457171 25.783115185168864 29.253908793990764 20.334591786268664 12-13 25.114949839847313 24.22483837099152 28.594807678201757 22.065404110959413 14-15 22.758913135790078 25.594808749562954 26.836646385325764 24.809631729321204 16-17 21.884061106917915 27.870037309914714 27.645024461898448 22.600877121268923 18-19 21.45464286385653 27.22658869032327 29.6867852734211 21.6319831723991 20-21 22.414690679330633 26.88892059036947 29.353121062438674 21.343267667861216 22-23 22.281222143368534 26.634298142134032 29.21118831983682 21.873291394660612 24-25 21.77469052698777 26.44607586360178 29.657065814969265 22.122167794441186 26-27 21.739084034563735 26.58399907182535 29.36471438313346 22.31220251047746 28-29 21.609576276156524 27.492669863497472 29.18950577643248 21.708248083913528 30-31 22.568280854886762 26.79445662181643 29.31039479357625 21.326867729720565 32-33 22.23584537557751 26.861349818197482 29.058078401019095 21.84472640520591 34-35 22.31768910226471 26.998386169110912 29.156064947244513 21.527859781379867 36-37 22.05197919876409 26.841539062730252 29.17605294482636 21.9304287936793 38-39 22.0342496267104 26.914145839962796 29.169823513573057 21.881781019753753 40-41 22.147100785237146 26.328635213641938 29.577220142810802 21.94704385831011 42-43 21.779345833432068 26.760307411324902 29.03068061844958 22.429666136793458 44-45 21.28965420258359 27.068426977823496 29.423285134213017 22.218633685379896 46-47 21.274642687157108 26.53119105586753 29.11497864055611 23.079187616419254 48-49 21.392870877604842 26.63048592998736 29.150697064152386 22.825946128255417 50-51 21.29995851223321 26.990793475816115 29.072613775329003 22.636634236621664 52-53 21.183674468710215 27.200509254725297 28.721623739104885 22.894192537459602 54-55 20.940157673249654 26.864396911718863 29.16200059209977 23.033444822931713 56-57 21.365505174510773 26.79237330006698 28.661079117575827 23.181042407846427 58-59 21.120230444182102 26.848290717574052 28.888231398489918 23.143247439753935 60-61 21.490818119954373 26.819157842172515 28.674480199352786 23.015543838520326 62-63 21.582040515626367 26.57683190639602 28.620933194384868 23.220194383592748 64-65 21.21726179985195 26.398308403930738 28.627081203661277 23.75734859255604 66-67 21.29149423049787 26.45686795434456 28.325470051456914 23.92616776370065 68-69 21.094541209198514 26.70159154752208 28.39778249170422 23.806084751575185 70-71 21.25637473054657 26.549912979713287 28.354981661693802 23.83873062804635 72-73 21.384844625361072 27.00224709417768 27.98783464638259 23.625073634078657 74-75 21.781838231168745 26.98886838230679 27.712976260310285 23.51631712621418 76-77 21.843019853507965 26.92016114496066 27.62616656260669 23.610652438924692 78-79 21.83088076842454 27.01715504689815 27.750618799461 23.40134538521631 80-81 22.062936645275776 27.045713163377222 27.545312610455575 23.346037580891423 82-83 22.50636894656351 26.938509558804814 27.653956187913785 22.901165306717893 84-85 22.72415324410763 27.127291716514613 27.094622139184736 23.053932900193022 86-87 22.51381819922702 27.16062182557363 27.300220956082228 23.025339019117123 88-89 22.684716902676154 26.644557676433372 27.33891325553478 23.331812165355693 90-91 23.32322026850486 26.557501570712 27.18774455939338 22.931533601389766 92-93 23.548630195933026 26.588800103705463 26.981279068640347 22.881290631721164 94-95 23.3805828759981 26.462736765836205 26.95748128638079 23.199199071784903 96-97 23.607565952996108 26.240310331791548 26.858077625834113 23.294046089378227 98-99 23.79666095471236 26.271392900856792 26.55805140758874 23.373894736842104 100 24.25263209843082 26.168701445884263 26.420802434332423 23.157864021352502 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2812.0 1 2017.0 2 2828.5 3 4160.0 4 2811.0 5 1917.0 6 2554.5 7 4075.5 8 6061.0 9 6989.0 10 6021.5 11 4980.5 12 5139.0 13 5507.0 14 5926.5 15 6506.0 16 7160.5 17 7909.5 18 8713.5 19 9455.5 20 10350.0 21 11635.5 22 13294.0 23 15433.0 24 18364.0 25 22008.5 26 26826.5 27 32688.0 28 39056.0 29 46038.0 30 54497.0 31 63406.0 32 73668.0 33 83674.0 34 92555.5 35 101920.5 36 112715.5 37 125320.0 38 133459.5 39 137363.5 40 141760.0 41 146387.5 42 151773.5 43 159700.0 44 168727.0 45 180298.0 46 193174.0 47 205505.5 48 216369.5 49 222919.0 50 225329.0 51 223239.0 52 214873.0 53 203674.5 54 190916.5 55 171260.0 56 150726.0 57 130439.0 58 109470.0 59 91165.0 60 71923.0 61 54670.5 62 42208.5 63 32431.0 64 23872.0 65 16988.5 66 12432.0 67 9532.5 68 7303.0 69 5401.5 70 4120.0 71 3184.5 72 2467.0 73 1853.0 74 1435.0 75 1088.5 76 791.5 77 542.0 78 357.5 79 261.0 80 198.5 81 153.5 82 103.0 83 76.0 84 68.0 85 57.0 86 40.0 87 26.0 88 27.0 89 29.5 90 21.0 91 13.5 92 12.5 93 10.0 94 6.0 95 3.5 96 3.5 97 3.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2229515154849303 2 0.1493587207266011 3 0.024354731758276547 4 0.023375843825869932 5 0.028759727454106307 6 0.0573236773217313 7 0.06398011526209627 8 0.05673634456228732 9 0.04359966850939057 10-11 0.04624266592688843 12-13 0.043863968251140355 14-15 0.021349545805788243 16-17 0.019920369424474588 18-19 0.019362403303002818 20-21 0.022915766497638823 22-23 0.026097152277960316 24-25 0.022945133135611022 26-27 0.020351080114733498 28-29 0.0197441695966414 30-31 0.01791364916304103 32-33 0.02612651891593252 34-35 0.026508285209571096 36-37 0.02571538598432174 38-39 0.023728243481536314 40-41 0.0252650975354147 42-43 0.03101116969864152 44-45 0.0405846936775782 46-47 0.04634055472012909 48-49 0.04255225842171549 50-51 0.029611359955300058 52-53 0.04575322196068512 54-55 0.044607923079769386 56-57 0.04656569894458261 58-59 0.0502365286911074 60-61 0.02568601934634954 62-63 0.027164140124283528 64-65 0.027741684004403428 66-67 0.03368353375411157 68-69 0.03770676315630275 70-71 0.04172020367916987 72-73 0.046095832737027435 74-75 0.026361452019710106 76-77 0.01970501407934513 78-79 0.019039370285308634 80-81 0.022436111410759584 82-83 0.020106358131631842 84-85 0.012510187776156522 86-87 0.013968730795442376 88-89 0.019881213907178324 90-91 0.021075457184714392 92-93 0.02087967959823307 94-95 0.02470713141394293 96-97 0.025500030639192286 98-99 0.031069902974585915 100 0.030345525904605024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5107837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.05750005278152 #Duplication Level Percentage of deduplicated Percentage of total 1 79.96366015095612 53.621631447933424 2 12.957967481195729 17.378578101084475 3 3.471728346615037 6.984162713591427 4 1.3822906717300572 3.7077182716999078 5 0.6425797632196101 2.1544896253007666 6 0.3772346175317083 1.5177846231026126 7 0.2582504306372332 1.2122339786260978 8 0.17675496366929908 0.9482196788466732 9 0.12683444432683033 0.7654680681426845 >10 0.6052259594507947 6.94885976060875 >50 0.025415629351426872 1.1358041622562958 >100 0.010646162362868694 1.3690127420012979 >500 8.533922912361292E-4 0.4061529326825546 >1k 4.266956827423174E-4 0.6187702822887067 >5k 3.282274482626349E-5 0.13131232896278325 >10k+ 9.846823447879048E-5 1.0998012828716648 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21678 0.4244066519742114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17433 0.3412990665128899 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10857 0.2125557256427721 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5966 0.11680090809475714 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.9577758648132273E-5 0.0 0.0 0.0 6 0.0 1.9577758648132273E-5 0.0 0.0 1.9577758648132273E-5 7 0.0 1.9577758648132273E-5 0.0 0.0 1.9577758648132273E-5 8 0.0 1.9577758648132273E-5 0.0 0.0 3.915551729626455E-5 9 0.0 1.9577758648132273E-5 0.0 3.915551729626455E-5 3.915551729626455E-5 10-11 0.0 1.9577758648132273E-5 0.0 3.915551729626455E-5 3.915551729626455E-5 12-13 0.0 1.9577758648132273E-5 0.0 9.788879324066136E-5 9.788879324066136E-5 14-15 0.0 1.9577758648132273E-5 0.0 2.3493310377758727E-4 1.1746655188879363E-4 16-17 0.0 1.9577758648132273E-5 0.0 2.5451086242571954E-4 1.7619982783319044E-4 18-19 0.0 1.9577758648132273E-5 0.0 4.0134405228671157E-4 1.8598870715725658E-4 20-21 0.0 1.9577758648132273E-5 0.0 9.005768978140845E-4 2.2514422445352113E-4 22-23 0.0 3.915551729626455E-5 0.0 0.0029366637972198404 2.936663797219841E-4 24-25 0.0 3.915551729626455E-5 0.0 0.007811525700604776 3.132441383701164E-4 26-27 9.788879324066137E-6 3.915551729626455E-5 0.0 0.016484472781727374 3.132441383701164E-4 28-29 1.9577758648132273E-5 4.894439662033068E-5 0.0 0.046144777133647764 3.132441383701164E-4 30-31 1.9577758648132273E-5 5.873327594439682E-5 0.0 0.13305044777270691 3.132441383701164E-4 32-33 1.9577758648132273E-5 5.873327594439682E-5 0.0 0.3169541236339374 3.230330176941825E-4 34-35 1.9577758648132273E-5 5.873327594439682E-5 0.0 0.6037976544670474 3.3282189701824865E-4 36-37 1.9577758648132273E-5 5.873327594439682E-5 0.0 1.0271373185949355 3.3282189701824865E-4 38-39 1.9577758648132273E-5 5.873327594439682E-5 0.0 1.621890048566546 3.7197741431451315E-4 40-41 1.9577758648132273E-5 5.873327594439682E-5 0.0 2.269179693870419 3.9155517296264543E-4 42-43 1.9577758648132273E-5 5.873327594439682E-5 0.0 2.8675249425539615 4.111329316107777E-4 44-45 1.9577758648132273E-5 5.873327594439682E-5 0.0 3.560597176456492 4.2092181093484384E-4 46-47 2.936663797219841E-5 5.873327594439682E-5 0.0 4.349316550234473 4.404995695829761E-4 48-49 3.915551729626455E-5 5.873327594439682E-5 0.0 5.148568758165149 4.5028844890704226E-4 50-51 3.915551729626455E-5 1.076776725647275E-4 0.0 5.930230349950478 4.99232845527373E-4 52-53 3.915551729626455E-5 1.566220691850582E-4 0.0 6.678355241171557 5.188106041755052E-4 54-55 3.915551729626455E-5 1.566220691850582E-4 0.0 7.490910536103638 5.481772421477036E-4 56-57 3.915551729626455E-5 1.566220691850582E-4 0.0 8.38599587261692 5.677550007958359E-4 58-59 3.915551729626455E-5 1.566220691850582E-4 0.0 9.348174188017355 5.775438801199021E-4 60-61 3.915551729626455E-5 1.566220691850582E-4 0.0 10.278401601304036 6.069105180921005E-4 62-63 4.894439662033068E-5 1.566220691850582E-4 0.0 11.198086391558697 6.852215526846295E-4 64-65 5.873327594439682E-5 1.566220691850582E-4 0.0 12.088854832290068 6.852215526846295E-4 66-67 5.873327594439682E-5 1.566220691850582E-4 0.0 13.058981717701641 6.852215526846295E-4 68-69 5.873327594439682E-5 1.566220691850582E-4 0.0 14.101086232783075 6.950104320086956E-4 70-71 5.873327594439682E-5 1.566220691850582E-4 0.0 15.151246995548213 7.047993113327617E-4 72-73 5.873327594439682E-5 1.566220691850582E-4 0.0 16.12879385148743 7.24377069980894E-4 74-75 5.873327594439682E-5 1.566220691850582E-4 0.0 17.081574842736757 7.537437079530925E-4 76-77 7.83110345925291E-5 1.7619982783319044E-4 0.0 18.080060111550154 7.831103459252909E-4 78-79 9.788879324066136E-5 1.7619982783319044E-4 0.0 19.128928742244515 7.831103459252909E-4 80-81 9.788879324066136E-5 1.7619982783319044E-4 0.0 20.16928104792694 8.124769838974892E-4 82-83 9.788879324066136E-5 1.7619982783319044E-4 0.0 21.183193590555064 9.005768978140845E-4 84-85 9.788879324066136E-5 1.7619982783319044E-4 0.0 22.16476367589647 9.005768978140845E-4 86-87 9.788879324066136E-5 1.7619982783319044E-4 0.0 23.14931936943172 9.397324151103491E-4 88 9.788879324066136E-5 1.7619982783319044E-4 0.0 23.899490136431528 9.593101737584813E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8290 0.0 52.22043 1 GTATCAA 15630 0.0 42.91711 1 TACCTGG 4920 0.0 42.662315 2 ACCTGGG 5970 0.0 35.117912 3 ATCAACG 19160 0.0 34.274338 3 CAACGCA 19425 0.0 33.80676 5 TATCAAC 19590 0.0 33.753326 2 TCAACGC 20030 0.0 32.85572 4 AACGCAG 20625 0.0 32.295967 6 GTACATG 14865 0.0 28.805698 1 ACGCAGA 22725 0.0 28.67083 7 CGCAGAG 23005 0.0 28.258354 8 TACATGG 15155 0.0 27.917624 2 TATCACG 510 0.0 27.683804 2 CCTGGGG 5720 0.0 27.119509 4 ACATGGG 15180 0.0 26.38372 3 GTGGTAT 3540 0.0 26.21455 1 GTACCTG 8370 0.0 25.776236 1 CATGGGG 11490 0.0 25.692331 4 GCAGAGT 25075 0.0 25.194223 9 >>END_MODULE