FastQCFastQC Report
Mon 6 Jun 2016
ERR1211384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107837
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198500.3886185091654256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165910.3248145937311625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108060.21155725995171734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86300.054.6983641
TACCTGG46500.044.5676882
GTATCAA156600.040.7117081
ACCTGGG58400.034.601453
ATCAACG193200.032.4722863
CAACGCA196100.032.059265
TATCAAC199850.031.6736372
TCAACGC199950.031.5177064
AACGCAG207500.030.545826
CCTGGGG49850.028.8471894
ATAACGC4800.028.3919663
TAACGCA5050.027.9175384
ACGCAGA228950.027.519857
CGCAGAG233250.026.8916578
GTACATG149350.026.0661281
TACATGG147950.025.8867242
GTACCTG85200.025.0534571
GTGGTAT34700.024.6600131
ACATGGG148350.024.296663
GCAGAGT252050.024.2893379
Produced by FastQC (version 0.11.5)