FastQCFastQC Report
Mon 6 Jun 2016
ERR1211383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915359
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267490.4521957162701368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217450.36760237206228735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125120.21151717080907517No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69430.11737241983115479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111050.054.7713051
TACCTGG50500.041.9357642
GTATCAA201300.040.8410641
ACCTGGG62050.033.8630183
ATCAACG243050.033.3040773
CAACGCA246000.032.9229745
TATCAAC249600.032.580862
TCAACGC256400.031.6608984
AACGCAG262250.031.1525946
GTACATG191200.028.0660481
ACGCAGA290450.027.8209387
TAACGCA6150.027.5154294
CGCAGAG293500.027.4988638
TACATGG191450.027.359192
GTGGTAT42950.026.9846861
ACATGGG196750.025.7312493
CCTGGGG61300.025.458134
GCAGAGT315100.024.9866459
ATAACGC7050.024.6701933
GTACTAG22200.024.6178651
Produced by FastQC (version 0.11.5)