##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211383_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5915359 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34899183633656 31.0 31.0 34.0 28.0 34.0 2 31.387671483674957 31.0 31.0 34.0 28.0 34.0 3 31.45186150155891 31.0 31.0 34.0 28.0 34.0 4 35.08568186647674 37.0 35.0 37.0 32.0 37.0 5 35.145450850912006 37.0 35.0 37.0 32.0 37.0 6 35.21003442056518 37.0 35.0 37.0 32.0 37.0 7 35.143636083625694 37.0 35.0 37.0 32.0 37.0 8 35.14964112913519 37.0 35.0 37.0 32.0 37.0 9 36.71466803620879 39.0 37.0 39.0 32.0 39.0 10-11 36.64654452925004 39.0 37.0 39.0 32.0 39.0 12-13 36.553854212398605 39.0 37.0 39.0 32.0 39.0 14-15 37.73308078174122 40.0 37.0 41.0 32.0 41.0 16-17 37.726675168827455 40.0 37.0 41.0 32.0 41.0 18-19 37.73320097732022 40.0 37.0 41.0 32.0 41.0 20-21 37.71712333942877 40.0 37.0 41.0 32.0 41.0 22-23 37.58832321081442 40.0 37.0 41.0 32.0 41.0 24-25 37.490511564894035 40.0 37.0 41.0 31.5 41.0 26-27 37.347503507394904 40.0 36.5 41.0 31.0 41.0 28-29 37.2084608896941 40.0 36.0 41.0 31.0 41.0 30-31 37.03733078584072 39.0 36.0 41.0 30.5 41.0 32-33 36.813397885064965 39.0 35.5 41.0 30.0 41.0 34-35 36.67216317724757 39.0 35.0 41.0 30.0 41.0 36-37 36.524658097674205 39.0 35.0 41.0 30.0 41.0 38-39 36.35750923992948 39.0 35.0 40.5 29.5 41.0 40-41 36.18361438418192 39.0 35.0 40.0 29.0 41.0 42-43 35.96763349443373 38.5 35.0 40.0 28.0 41.0 44-45 35.81465630403835 38.0 35.0 40.0 27.0 41.0 46-47 35.56555823577233 38.0 34.5 40.0 26.5 41.0 48-49 35.29893173009449 38.0 34.0 40.0 25.5 41.0 50-51 34.618469056569516 37.5 33.5 39.5 24.5 40.5 52-53 34.90935309251729 38.0 33.5 39.5 25.5 40.5 54-55 35.312073874130036 38.0 34.0 40.0 26.0 41.0 56-57 35.23052675247605 38.0 34.0 40.0 26.0 41.0 58-59 35.02584771270856 38.0 34.0 40.0 25.0 41.0 60-61 34.801574257792296 38.0 34.0 40.0 25.0 41.0 62-63 34.51687665955693 37.0 33.0 40.0 24.5 41.0 64-65 34.30016048053888 37.0 33.0 40.0 24.0 41.0 66-67 34.09481723763511 36.0 33.0 39.5 24.0 41.0 68-69 33.75508485621921 36.0 33.0 39.0 23.0 41.0 70-71 33.38046160850085 35.0 32.0 39.0 23.0 41.0 72-73 32.99088550669536 35.0 32.0 38.5 22.0 40.0 74-75 32.60776691659797 35.0 32.0 37.0 21.0 40.0 76-77 32.212442896534256 35.0 31.0 37.0 20.0 39.0 78-79 31.80049909058774 35.0 31.0 36.0 20.0 39.0 80-81 31.38454834271259 35.0 30.5 36.0 18.5 38.0 82-83 31.002479139474037 34.5 30.0 35.5 18.0 37.0 84-85 30.65279774228411 34.0 30.0 35.0 17.0 37.0 86-87 30.302114546217737 34.0 29.0 35.0 15.0 36.0 88-89 29.963989167859467 34.0 29.0 35.0 11.5 36.0 90-91 29.643369489493367 34.0 29.0 35.0 10.5 36.0 92-93 29.33634442136141 34.0 27.5 35.0 10.0 35.0 94-95 29.035332428682686 34.0 27.0 35.0 10.0 35.0 96-97 28.7259248339788 34.0 26.0 35.0 10.0 35.0 98-99 28.388511246739206 33.5 25.0 35.0 8.0 35.0 100 27.11831285303225 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33043828311138057 1101 2 -0.346811898334618 1101 3 -0.4046362059020794 1101 4 -0.21059030463430162 1101 5 -0.2878768928770583 1101 6 -0.33441683518181264 1101 7 -0.28754148795552226 1101 8 -0.3173835600852968 1101 9 -0.5009534790884018 1101 10-11 -0.44692941252814933 1101 12-13 -0.46306499492802544 1101 14-15 -0.6659996125231871 1101 16-17 -0.6106377293266334 1101 18-19 -0.6287894938054137 1101 20-21 -0.6483011462629591 1101 22-23 -0.6826679480860918 1101 24-25 -0.708406089060098 1101 26-27 -0.7573901436296637 1101 28-29 -0.719623050165275 1101 30-31 -0.7883937560634919 1101 32-33 -0.8763865857132132 1101 34-35 -0.8216881459453589 1101 36-37 -0.8468617414509296 1101 38-39 -0.7935759948585641 1101 40-41 -0.9783239490922071 1101 42-43 -1.0124981503380397 1101 44-45 -0.9342737623150299 1101 46-47 -0.9876320451102387 1101 48-49 -1.0264402794836727 1101 50-51 -0.8189480725107998 1101 52-53 -0.7218541134175993 1101 54-55 -0.6713790519266709 1101 56-57 -0.6946558046860645 1101 58-59 -0.6031173119747208 1101 60-61 -0.6775683335802469 1101 62-63 -0.746318527264684 1101 64-65 -0.7101560842905528 1101 66-67 -0.8253753999353037 1101 68-69 -0.8888715504921052 1101 70-71 -0.7894331064371016 1101 72-73 -0.9259775991945745 1101 74-75 -0.9234482878640335 1101 76-77 -0.9496893278961025 1101 78-79 -0.9709438984082652 1101 80-81 -1.0943787082284402 1101 82-83 -1.1499844003525759 1101 84-85 -1.1685745835631671 1101 86-87 -1.294539297228102 1101 88-89 -1.4450654254973152 1101 90-91 -1.4981545232077238 1101 92-93 -1.5243063196673248 1101 94-95 -1.531599319296209 1101 96-97 -1.7424284304715414 1101 98-99 -1.7552670684709852 1101 100 -1.9600301766405472 1102 1 -0.2750508954605806 1102 2 -0.2852279949134413 1102 3 -0.32284030939749186 1102 4 -0.2823420154392906 1102 5 -0.2083070665579143 1102 6 -0.19625520367067395 1102 7 -0.24225794793404276 1102 8 -0.2722866201094618 1102 9 -0.38301324849758345 1102 10-11 -0.41675930868458266 1102 12-13 -0.457526452441563 1102 14-15 -0.6394634548895297 1102 16-17 -0.5407900564193895 1102 18-19 -0.6257499841391763 1102 20-21 -0.6502355058220886 1102 22-23 -0.5885255516081713 1102 24-25 -0.6538575386656333 1102 26-27 -0.6274725860611525 1102 28-29 -0.7445407891223041 1102 30-31 -0.6084479445272422 1102 32-33 -0.6169006603786826 1102 34-35 -0.6498102652183206 1102 36-37 -0.6104349827471935 1102 38-39 -0.7139533763611112 1102 40-41 -0.8250946346243566 1102 42-43 -0.8351147391565448 1102 44-45 -0.7836477621401343 1102 46-47 -0.8461469260070302 1102 48-49 -0.921561121456655 1102 50-51 -0.7797526550884584 1102 52-53 -0.6435445649910605 1102 54-55 -0.5861372475062652 1102 56-57 -0.6068117350480406 1102 58-59 -0.6027612693413857 1102 60-61 -0.6091745606478014 1102 62-63 -0.6611836946876295 1102 64-65 -0.7147312372051289 1102 66-67 -0.6832816140890756 1102 68-69 -0.7678109975928535 1102 70-71 -0.8098277739939306 1102 72-73 -0.7778884662343586 1102 74-75 -0.9825929896004055 1102 76-77 -0.8717628611737211 1102 78-79 -0.9657190802096345 1102 80-81 -1.0082740901040488 1102 82-83 -0.9736105211372887 1102 84-85 -1.1269409796902252 1102 86-87 -1.2124957174202713 1102 88-89 -1.1873615576716041 1102 90-91 -1.22851735572738 1102 92-93 -1.2454225683146944 1102 94-95 -1.2517887944851012 1102 96-97 -1.5214026391912405 1102 98-99 -1.5442050732623471 1102 100 -1.4495944489518102 1103 1 -0.43619658242270987 1103 2 -0.4460119375361131 1103 3 -0.5507784436879923 1103 4 -0.3745250641270488 1103 5 -0.34930469417526666 1103 6 -0.4171273568940208 1103 7 -0.4320949126487008 1103 8 -0.3575464598305871 1103 9 -0.5621105326916336 1103 10-11 -0.5806309386853172 1103 12-13 -0.6562657665412814 1103 14-15 -0.8039524573114321 1103 16-17 -0.86646573432553 1103 18-19 -0.8303539579901411 1103 20-21 -0.6788765795122984 1103 22-23 -0.7552063396001287 1103 24-25 -0.894595439349068 1103 26-27 -0.8754418661266428 1103 28-29 -1.0043260422773201 1103 30-31 -0.9789689856687147 1103 32-33 -1.08847009988677 1103 34-35 -1.0273454328201126 1103 36-37 -1.0133298727714148 1103 38-39 -0.9450099394212614 1103 40-41 -0.9146606762114757 1103 42-43 -0.9736463157236486 1103 44-45 -0.9759064882939015 1103 46-47 -1.0926039634336675 1103 48-49 -0.9365423575164229 1103 50-51 -0.8510889139202433 1103 52-53 -0.7037791238720317 1103 54-55 -0.787886879709923 1103 56-57 -0.7853591816006542 1103 58-59 -0.7730889354384232 1103 60-61 -0.824481696721989 1103 62-63 -0.7942914444342719 1103 64-65 -0.7151021970847893 1103 66-67 -0.7736470694027915 1103 68-69 -0.8107617420434465 1103 70-71 -0.853778198238885 1103 72-73 -0.7860684273035119 1103 74-75 -0.7929084251626008 1103 76-77 -0.947380494931096 1103 78-79 -0.9607734298895956 1103 80-81 -0.9900888032779278 1103 82-83 -1.02404008910176 1103 84-85 -1.179283047338341 1103 86-87 -1.2208814463722533 1103 88-89 -1.15032567703458 1103 90-91 -1.1622361645624153 1103 92-93 -1.2887721943563264 1103 94-95 -1.3002264990838484 1103 96-97 -1.3452023357111216 1103 98-99 -1.4063501511735481 1103 100 -1.3617271615336897 1104 1 -0.37001365128995545 1104 2 -0.3528099209326392 1104 3 -0.36102297693980745 1104 4 -0.21417059278966377 1104 5 -0.1865257994792131 1104 6 -0.2408015315263654 1104 7 -0.24041244007108276 1104 8 -0.47751521966466726 1104 9 -0.4427046631577696 1104 10-11 -0.43062235876830357 1104 12-13 -0.47755801368052886 1104 14-15 -0.641490956353941 1104 16-17 -0.7197332152745304 1104 18-19 -0.7141184625785684 1104 20-21 -0.6843099769586303 1104 22-23 -0.6471938907162809 1104 24-25 -0.6638613902362778 1104 26-27 -0.7515275029664181 1104 28-29 -0.889920308954899 1104 30-31 -0.8825450768242149 1104 32-33 -0.8300376984691056 1104 34-35 -0.816889178531035 1104 36-37 -0.8269923479637313 1104 38-39 -0.9074670451021021 1104 40-41 -0.8636267224888243 1104 42-43 -0.8509689491094221 1104 44-45 -0.9158229338041934 1104 46-47 -0.936308575707784 1104 48-49 -0.8988969535504836 1104 50-51 -0.8888295254759484 1104 52-53 -0.7765381366114568 1104 54-55 -0.7074828590007272 1104 56-57 -0.5780025276503338 1104 58-59 -0.6676373846570414 1104 60-61 -0.6474726375372768 1104 62-63 -0.7532838820308214 1104 64-65 -0.6923158823754036 1104 66-67 -0.6411036056636092 1104 68-69 -0.7142097487995329 1104 70-71 -0.7073309333437052 1104 72-73 -0.6247510323011269 1104 74-75 -0.7729393177786861 1104 76-77 -0.7609112171424499 1104 78-79 -0.7493724895250757 1104 80-81 -0.7362848879430288 1104 82-83 -0.8058048095221473 1104 84-85 -0.8381388416820847 1104 86-87 -0.9074164426741831 1104 88-89 -0.9788892985234661 1104 90-91 -1.0302347556866813 1104 92-93 -0.9631147389069454 1104 94-95 -0.9675176788589503 1104 96-97 -0.993242993705099 1104 98-99 -1.0821405742729908 1104 100 -1.1735197659822418 1105 1 -0.5248724612421931 1105 2 -0.3863301890567037 1105 3 -0.41277755860777177 1105 4 -0.34746154075304503 1105 5 -0.4596325169122224 1105 6 -0.3359107146413862 1105 7 -0.41457289713578405 1105 8 -0.4414623223206817 1105 9 -0.4882476034877641 1105 10-11 -0.5376802939146685 1105 12-13 -0.6299839782675676 1105 14-15 -0.754316609504059 1105 16-17 -0.7187542842004859 1105 18-19 -0.6181076209529692 1105 20-21 -0.7320044804656476 1105 22-23 -0.7303030637741372 1105 24-25 -0.7226548151959875 1105 26-27 -0.7613434987222902 1105 28-29 -0.762872984119312 1105 30-31 -0.8397296400791845 1105 32-33 -0.8755865222415693 1105 34-35 -0.814337926790941 1105 36-37 -0.8653086274155086 1105 38-39 -0.8508145562467107 1105 40-41 -0.9671353159186467 1105 42-43 -0.9062263245744546 1105 44-45 -0.8511919438186126 1105 46-47 -0.9385696614292058 1105 48-49 -0.9946019735013891 1105 50-51 -0.9768883163195667 1105 52-53 -0.7451368129207125 1105 54-55 -0.7236515478562993 1105 56-57 -0.849711717972994 1105 58-59 -0.7401184792790545 1105 60-61 -0.762641861936963 1105 62-63 -0.8858059093209434 1105 64-65 -0.8475823428142775 1105 66-67 -0.8387172411701016 1105 68-69 -0.8344783757197192 1105 70-71 -0.8055406571152446 1105 72-73 -0.771308170836086 1105 74-75 -0.7988945348919643 1105 76-77 -0.8558958757728803 1105 78-79 -0.8804209072394329 1105 80-81 -0.893144319763401 1105 82-83 -0.9014490339008852 1105 84-85 -0.8207265171272589 1105 86-87 -0.9781091384055038 1105 88-89 -1.016929559037603 1105 90-91 -0.9716100732207629 1105 92-93 -0.9011520996047118 1105 94-95 -0.8615531588665597 1105 96-97 -0.93721882633373 1105 98-99 -1.0646910717255906 1105 100 -0.9015751541511605 1106 1 -0.44721014163548034 1106 2 -0.5322627538544076 1106 3 -0.5447009184373144 1106 4 -0.3762566340232638 1106 5 -0.330057798080837 1106 6 -0.3461894664257201 1106 7 -0.48502937884628494 1106 8 -0.438552524218899 1106 9 -0.6046322851533219 1106 10-11 -0.6879840061325098 1106 12-13 -0.6044840663727271 1106 14-15 -0.7894136625886361 1106 16-17 -0.6935740566143593 1106 18-19 -0.7887234517522614 1106 20-21 -0.8244945585316614 1106 22-23 -0.7801473520542075 1106 24-25 -0.8679590851437169 1106 26-27 -0.8412682294357481 1106 28-29 -0.8994455965099704 1106 30-31 -0.8960781097168606 1106 32-33 -0.9477686525757676 1106 34-35 -0.7844094132138508 1106 36-37 -0.8473288220874977 1106 38-39 -0.9536555999937448 1106 40-41 -0.9017584117560418 1106 42-43 -0.773055072398094 1106 44-45 -0.8139417946864853 1106 46-47 -0.757779689574356 1106 48-49 -0.7167539217569825 1106 50-51 -0.8368267397060762 1106 52-53 -0.8046193527418168 1106 54-55 -0.6536946143037525 1106 56-57 -0.7254176504407965 1106 58-59 -0.6202850869395178 1106 60-61 -0.6162854547521093 1106 62-63 -0.6538424091051311 1106 64-65 -0.6858844351905162 1106 66-67 -0.619835028873851 1106 68-69 -0.48776599136784426 1106 70-71 -0.5693432110331713 1106 72-73 -0.5393880765554826 1106 74-75 -0.5653447861937373 1106 76-77 -0.5805984602447118 1106 78-79 -0.5893974971333655 1106 80-81 -0.648833762825646 1106 82-83 -0.609895036755816 1106 84-85 -0.6823137517407858 1106 86-87 -0.8055813933640472 1106 88-89 -0.8321692616463245 1106 90-91 -0.7749694501152256 1106 92-93 -0.8089945417180076 1106 94-95 -0.7740637516930207 1106 96-97 -0.8210915572217807 1106 98-99 -0.8984513998324317 1106 100 -0.9175449817722061 1107 1 -0.4862342214616433 1107 2 -0.45487536742441037 1107 3 -0.508826486350582 1107 4 -0.41838488045283384 1107 5 -0.3515564590757023 1107 6 -0.4527673923745894 1107 7 -0.3907169372316659 1107 8 -0.4135568129824918 1107 9 -0.5541847229422103 1107 10-11 -0.4775504437614799 1107 12-13 -0.5926060572800438 1107 14-15 -0.7067733314606599 1107 16-17 -0.738046004811153 1107 18-19 -0.7387788071655166 1107 20-21 -0.7318805748345341 1107 22-23 -0.6847832145182409 1107 24-25 -0.7469847666143394 1107 26-27 -0.7791410292620711 1107 28-29 -0.7476502808519072 1107 30-31 -0.7341230104149048 1107 32-33 -0.725517142779168 1107 34-35 -0.8467293230966817 1107 36-37 -0.7473324087083739 1107 38-39 -0.8171551981909957 1107 40-41 -0.8124811557145861 1107 42-43 -0.7560771667417825 1107 44-45 -0.8058570957804676 1107 46-47 -0.7616233832448032 1107 48-49 -0.9290232077828549 1107 50-51 -1.001372777242743 1107 52-53 -0.7777570172456265 1107 54-55 -0.7518561579853795 1107 56-57 -0.6061858793307167 1107 58-59 -0.6983731611910429 1107 60-61 -0.6471911517989071 1107 62-63 -0.6592379014248237 1107 64-65 -0.7350082686215202 1107 66-67 -0.7534470938869546 1107 68-69 -0.6735007982646763 1107 70-71 -0.5850669379915132 1107 72-73 -0.6518610915031502 1107 74-75 -0.5623418609786413 1107 76-77 -0.6022671536368307 1107 78-79 -0.6260883440768836 1107 80-81 -0.5518973000359182 1107 82-83 -0.5864395483023301 1107 84-85 -0.5845836146622858 1107 86-87 -0.5660098889214424 1107 88-89 -0.6179073768372021 1107 90-91 -0.6438429703020176 1107 92-93 -0.5661578838970769 1107 94-95 -0.6172475463404439 1107 96-97 -0.6510916103569038 1107 98-99 -0.6146408877818921 1107 100 -0.659802308599776 1108 1 -0.5155227622190139 1108 2 -0.4700194528655537 1108 3 -0.5236005710730822 1108 4 -0.38631401595526427 1108 5 -0.42230031379721567 1108 6 -0.5154182509018881 1108 7 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1.1199410477833567 2311 56-57 1.1609304868809716 2311 58-59 1.160340591062365 2311 60-61 1.0500251886595038 2311 62-63 1.0518464148254978 2311 64-65 1.0273418858981813 2311 66-67 1.0085824708825513 2311 68-69 1.0885393124744454 2311 70-71 1.0717045917535302 2311 72-73 1.1156601549557763 2311 74-75 1.104338373376244 2311 76-77 1.1512695705455016 2311 78-79 1.138368068490717 2311 80-81 1.154745437538125 2311 82-83 1.2135278480407017 2311 84-85 1.1926279871919512 2311 86-87 1.1811793848359606 2311 88-89 1.2633623373013485 2311 90-91 1.2866777095846658 2311 92-93 1.308525234825563 2311 94-95 1.3738584412979833 2311 96-97 1.4315933497255031 2311 98-99 1.4073658595783982 2311 100 1.6571401070582183 2312 1 0.6071423640153171 2312 2 0.6546281904296123 2312 3 0.718084118881162 2312 4 0.5517277476410953 2312 5 0.5835783662040726 2312 6 0.4488383701490761 2312 7 0.6305427911959853 2312 8 0.7128596430329139 2312 9 0.9121440119910531 2312 10-11 0.9340054132247815 2312 12-13 0.9848078866809615 2312 14-15 1.1858606829049805 2312 16-17 1.1241812077388502 2312 18-19 1.0777185038533545 2312 20-21 1.0947474433421505 2312 22-23 1.1096515249419028 2312 24-25 1.1749237693882222 2312 26-27 1.254157990624897 2312 28-29 1.2539720685519242 2312 30-31 1.1713634465563132 2312 32-33 1.3133182472425702 2312 34-35 1.3619708959264045 2312 36-37 1.351252393591274 2312 38-39 1.4108066720668049 2312 40-41 1.380215075339514 2312 42-43 1.408710284781307 2312 44-45 1.4470732022088697 2312 46-47 1.407936170899859 2312 48-49 1.4579277728385804 2312 50-51 1.371602034594666 2312 52-53 1.2708484137946385 2312 54-55 1.1940578134297724 2312 56-57 1.15989481501515 2312 58-59 1.1898982494843438 2312 60-61 1.2467850398635676 2312 62-63 1.2810402802565477 2312 64-65 1.2695094509964022 2312 66-67 1.2157739607996731 2312 68-69 1.258887938722566 2312 70-71 1.2533200978554717 2312 72-73 1.3028389058531147 2312 74-75 1.264461482427997 2312 76-77 1.2847712608150346 2312 78-79 1.255638315831316 2312 80-81 1.1341815792858227 2312 82-83 1.3295292576863353 2312 84-85 1.3354878449221594 2312 86-87 1.418791915019348 2312 88-89 1.474787201884002 2312 90-91 1.4754553118818663 2312 92-93 1.5206282955839008 2312 94-95 1.542235077726879 2312 96-97 1.4988073939727151 2312 98-99 1.4015952402479783 2312 100 1.649328686318153 2313 1 0.6429171331191519 2313 2 0.6295013429681617 2313 3 0.700951646286665 2313 4 0.6334433725759965 2313 5 0.6303989619540289 2313 6 0.6516116135778276 2313 7 0.6704290082482913 2313 8 0.7086431302800875 2313 9 0.8592321978399227 2313 10-11 0.7388686232187425 2313 12-13 0.8234129532634924 2313 14-15 1.08387593355922 2313 16-17 1.0542405632567053 2313 18-19 1.0484234592236703 2313 20-21 1.1061053144894046 2313 22-23 1.1367928426923228 2313 24-25 1.2097769352275236 2313 26-27 1.1879508046596854 2313 28-29 1.2027106558367535 2313 30-31 1.2171101763634766 2313 32-33 1.2625672726750636 2313 34-35 1.2068252861669606 2313 36-37 1.2687614330764276 2313 38-39 1.2965762844599809 2313 40-41 1.3112523769014146 2313 42-43 1.3421293770071756 2313 44-45 1.303155758352247 2313 46-47 1.3778597448915448 2313 48-49 1.391436332724254 2313 50-51 1.3447011262515147 2313 52-53 1.2544846721260328 2313 54-55 1.2006242620920489 2313 56-57 1.2179005956115958 2313 58-59 1.2208817993233225 2313 60-61 1.1872779197924856 2313 62-63 1.1692305491624992 2313 64-65 1.1297440991131396 2313 66-67 1.182958279928421 2313 68-69 1.088013802598617 2313 70-71 1.1411641812793647 2313 72-73 1.1849029008864207 2313 74-75 1.147538682196931 2313 76-77 1.1001560403389803 2313 78-79 1.099592542530484 2313 80-81 1.1497785714247613 2313 82-83 1.114244157633447 2313 84-85 1.213998739299008 2313 86-87 1.257541013865108 2313 88-89 1.3196005924644467 2313 90-91 1.3955065843192713 2313 92-93 1.3616220115096027 2313 94-95 1.4048346492372445 2313 96-97 1.4672899848667278 2313 98-99 1.5106424985798768 2313 100 1.6270744569263265 2314 1 0.5010021974035972 2314 2 0.41321689906926906 2314 3 0.5490097749149285 2314 4 0.4572138208948715 2314 5 0.45112045324609795 2314 6 0.4470678625166826 2314 7 0.5166944751405183 2314 8 0.4866549541295839 2314 9 0.6816840134620037 2314 10-11 0.6320994889394953 2314 12-13 0.3922735536461275 2314 14-15 0.7852824664110614 2314 16-17 0.8449492109973136 2314 18-19 0.8552908531400192 2314 20-21 0.8549237067519542 2314 22-23 0.6635856523493189 2314 24-25 0.7821617752437291 2314 26-27 0.8384440233106432 2314 28-29 0.908892157773117 2314 30-31 0.8718883997092917 2314 32-33 0.957522415516074 2314 34-35 0.9909753688873693 2314 36-37 1.0954022100636607 2314 38-39 1.1419056272365893 2314 40-41 1.1257778148452573 2314 42-43 1.2329695189671739 2314 44-45 1.1820973482228538 2314 46-47 1.158757943073752 2314 48-49 1.1621163710453075 2314 50-51 1.1025230316119874 2314 52-53 0.9545835635611652 2314 54-55 1.0135591033568048 2314 56-57 0.9783397594278966 2314 58-59 1.0408897298929247 2314 60-61 0.9745440905879548 2314 62-63 1.0019243825429172 2314 64-65 0.9576819273785944 2314 66-67 0.9444100154224699 2314 68-69 0.9984050550640049 2314 70-71 1.038286652119126 2314 72-73 1.009079454941606 2314 74-75 1.0520150013536167 2314 76-77 1.0353732148960475 2314 78-79 1.0476595263597623 2314 80-81 1.0601043976937348 2314 82-83 0.9369417426907596 2314 84-85 0.9251669027754446 2314 86-87 1.2102502390490564 2314 88-89 1.2827591554187592 2314 90-91 1.3012749605411642 2314 92-93 1.4028999343502129 2314 94-95 1.4086974122745737 2314 96-97 1.4556365202718347 2314 98-99 1.4825000680367921 2314 100 1.43067939622307 2315 1 0.5029990659884227 2315 2 0.5226502481239095 2315 3 0.5275201484326928 2315 4 0.38671663823637203 2315 5 0.3783744067400079 2315 6 -0.22396608885128444 2315 7 0.2581447114027213 2315 8 0.34830187829514614 2315 9 0.6026805611190653 2315 10-11 0.6316155221282926 2315 12-13 0.7000916616324773 2315 14-15 0.811829407519916 2315 16-17 0.7263351504763023 2315 18-19 0.8114260330495213 2315 20-21 0.843411001200927 2315 22-23 0.7496980618196147 2315 24-25 0.8256526169744873 2315 26-27 0.9322891800157365 2315 28-29 1.0127678539227603 2315 30-31 0.9673665906825377 2315 32-33 1.059044370554382 2315 34-35 1.0682774027954167 2315 36-37 1.114147735816097 2315 38-39 1.1245259733826813 2315 40-41 1.1787356435124536 2315 42-43 1.2215574313567998 2315 44-45 1.2067518965571864 2315 46-47 1.2036862035395686 2315 48-49 1.1928835376057236 2315 50-51 1.1075481614224358 2315 52-53 1.0271104850289632 2315 54-55 0.878309086378259 2315 56-57 0.8189374319535929 2315 58-59 0.7614841661163894 2315 60-61 0.7115732865538646 2315 62-63 0.8705799592247132 2315 64-65 0.9981885013059895 2315 66-67 0.9853422377050052 2315 68-69 1.0325202160374332 2315 70-71 1.0211159792604434 2315 72-73 1.0246955336180221 2315 74-75 1.0737136284141826 2315 76-77 1.0416893504339413 2315 78-79 0.9854930998993332 2315 80-81 0.998668498998498 2315 82-83 1.1470084149033575 2315 84-85 1.1692477067758489 2315 86-87 1.2004566550505444 2315 88-89 1.4080091336227731 2315 90-91 1.4683408383851102 2315 92-93 1.5745070731088937 2315 94-95 1.5496681603793405 2315 96-97 1.62645567446544 2315 98-99 1.7025612663827587 2315 100 1.8818103316926234 2316 1 0.006324249472356769 2316 2 -0.05175729810965635 2316 3 0.05043564092172659 2316 4 -0.00365406568231208 2316 5 0.029294756393063892 2316 6 0.11386982304657067 2316 7 0.11762373940029391 2316 8 0.1975653038622056 2316 9 0.31493825597581804 2316 10-11 0.22642092791075896 2316 12-13 0.2722496415359714 2316 14-15 0.4460087160864461 2316 16-17 0.4703963722789979 2316 18-19 0.4484224160303043 2316 20-21 0.4422602519090191 2316 22-23 0.3394910379775311 2316 24-25 0.34590029411129564 2316 26-27 0.4074551279162577 2316 28-29 0.5800106510497613 2316 30-31 0.5926721529204855 2316 32-33 0.5989617838400036 2316 34-35 0.5586653476588168 2316 36-37 0.6051168391141033 2316 38-39 0.7007142620902087 2316 40-41 0.6525086274089986 2316 42-43 0.6428281118281021 2316 44-45 0.5894036033535954 2316 46-47 0.5066838699959746 2316 48-49 0.6307655808343426 2316 50-51 0.39015948640808773 2316 52-53 0.2017916684340051 2316 54-55 0.1901553973681942 2316 56-57 0.264147999936867 2316 58-59 0.3379878502641134 2316 60-61 0.3860647833945592 2316 62-63 0.4119782737091455 2316 64-65 0.47347417058553276 2316 66-67 0.44725794083208115 2316 68-69 0.4447188012595191 2316 70-71 0.35865805641808635 2316 72-73 0.45238973203250765 2316 74-75 0.5346866958348855 2316 76-77 0.5414951613481449 2316 78-79 0.6308177672972128 2316 80-81 0.6756110714220753 2316 82-83 0.738887524429888 2316 84-85 0.8594481201929902 2316 86-87 0.8787149632402027 2316 88-89 0.9753221815072912 2316 90-91 1.006189157442499 2316 92-93 1.1184950228003672 2316 94-95 1.13001465615352 2316 96-97 1.2202220163337287 2316 98-99 1.2977794953158508 2316 100 1.112372518234313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20.0 1 353.0 2 177.0 3 113.0 4 114.0 5 64.0 6 113.0 7 470.0 8 1642.0 9 3198.0 10 4625.0 11 6373.0 12 8553.0 13 10220.0 14 12679.0 15 15995.0 16 21373.0 17 28522.0 18 36430.0 19 44646.0 20 53555.0 21 63085.0 22 68960.0 23 69163.0 24 68092.0 25 70281.0 26 77558.0 27 89301.0 28 104844.0 29 123813.0 30 149981.0 31 184585.0 32 229996.0 33 295836.0 34 397446.0 35 539890.0 36 762662.0 37 1083535.0 38 1013979.0 39 272772.0 40 345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.63082238220884 18.683923490598293 12.208751705651121 23.476502421541742 2 16.438831589172946 20.8589381857188 39.72500650949303 22.97722371561523 3 18.95310527960435 26.818097886886683 30.265163864017282 23.96363296949168 4 12.603952591091568 17.003771055567523 38.499278152801644 31.892998200539264 5 12.727285332916672 37.489306532320015 34.79768948385068 14.985718650912624 6 29.28996034530236 37.09446288892693 19.224984421610422 14.390592344160286 7 26.12648790997028 33.22648777464423 23.140383189256195 17.506641126129296 8 25.684871437282386 34.47288989774353 21.29759416924119 18.5446444957329 9 27.066954652395236 15.550502106195838 20.951672077815005 36.43087116359393 10-11 24.573487589775507 25.798113033632042 29.195168337796918 20.433231038795537 12-13 25.245390215578077 24.314837538675782 28.614112168792445 21.825660076953696 14-15 22.76750613700148 25.636865024519263 26.90733170040679 24.688297138072475 16-17 21.723762126210232 28.01471914180836 27.683169846962485 22.57834888501892 18-19 21.273772592178965 27.356673318687896 29.863122792141173 21.506431296991966 20-21 22.361121197730725 26.848925283948795 29.561981251259184 21.227972267061297 22-23 22.266475161641548 26.72105531127993 29.260734576436327 21.751734950642195 24-25 21.761073052913726 26.549397180473182 29.763197488282973 21.926332278330115 26-27 21.689420875808576 26.764029268298454 29.50565171286199 22.040898143030972 28-29 21.43165721738951 27.477461483014885 29.44189155303778 21.648989746557824 30-31 22.34052185431158 26.845516390025654 29.52178642542368 21.29217533023909 32-33 22.144483773042843 26.973894744495862 29.171237506547133 21.71038397591416 34-35 22.215620942513375 27.047340588465712 29.34257651526213 21.394461953758785 36-37 21.983355769248654 26.82719689489863 29.331931095671354 21.85751624018136 38-39 21.818477112521254 26.97297046859845 29.374916299964692 21.8336361189156 40-41 21.899118091348495 26.389808679822206 29.803475165306615 21.907598063522684 42-43 21.591575697057884 26.70006704877989 29.262784714231 22.445572539931227 44-45 21.218536424992408 27.134058895036517 29.517719178735497 22.129685501235578 46-47 21.187305376825645 26.62739306591836 29.277862191063726 22.907439366192275 48-49 21.349002208895076 26.682787885696236 29.278844210575805 22.689365694832883 50-51 21.293189411888246 26.950546435566697 29.083800314828867 22.672463837716187 52-53 21.03783927129628 27.27001126041944 28.809651217894363 22.882498250389922 54-55 20.857969464431417 26.950437013064604 29.169804980187507 23.021788542316475 56-57 21.334671586486202 26.78583478938411 28.773113660921783 23.106379963207903 58-59 21.03495003296407 26.82507461313306 28.97134172392109 23.168633629981777 60-61 21.37094449163082 26.818042777516904 28.733115417386934 23.077897313465343 62-63 21.347141296154067 26.564661774408954 28.744694220365336 23.343502709071647 64-65 21.06470018528766 26.42968052146027 28.697055308557008 23.80856398469506 66-67 21.24203125040955 26.457952371784483 28.37586362910366 23.92415274870231 68-69 21.012614879622138 26.711887107861916 28.43645335775305 23.8390446547629 70-71 21.189781855766242 26.56634125044827 28.4225505820531 23.821326311732392 72-73 21.43621358321834 26.867511978044522 28.09575095476799 23.60052348396915 74-75 21.811749015935515 26.969382377735844 27.78446686012727 23.434401746201363 76-77 21.836357240388246 26.88563626324676 27.683880430429625 23.594126065935374 78-79 21.818324769994568 27.009225579689776 27.740643909532253 23.431805740783403 80-81 21.943889368157667 27.11907065518992 27.588482579434714 23.3485573972177 82-83 22.39909763050228 26.925680822466987 27.683888981227746 22.99133256580299 84-85 22.63902424014126 27.12550966650278 27.143930023271025 23.091536070084935 86-87 22.48883260574787 27.198133751737245 27.31401765817798 22.999015984336904 88-89 22.75395349128192 26.66387170004141 27.307973703303723 23.274201105372946 90-91 23.350029446322896 26.53854289482971 27.155005004099493 22.95642265474791 92-93 23.543501642590723 26.585283083758853 27.000669413420358 22.870545860230063 94-95 23.357525441704237 26.460970335710105 26.985521597210106 23.195982625375557 96-97 23.663172564974786 26.227051621518292 26.829668756724658 23.280107056782267 98-99 23.79908814633355 26.30796668368124 26.586284721796527 23.306660448188683 100 24.345384709821012 26.150537758052334 26.357449727121736 23.146627805004915 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3241.0 1 2312.5 2 3193.5 3 4630.0 4 3033.0 5 2068.0 6 2891.0 7 4603.5 8 6880.0 9 7993.0 10 6864.0 11 5605.0 12 5686.5 13 6256.5 14 6923.0 15 7513.5 16 8168.5 17 9037.0 18 9986.0 19 10947.5 20 12271.5 21 13857.0 22 15595.5 23 18116.0 24 21297.5 25 25369.5 26 30448.0 27 36565.0 28 43813.0 29 52738.5 30 63459.0 31 74167.0 32 87022.0 33 99421.5 34 110825.5 35 122464.5 36 134891.0 37 150797.5 38 160752.0 39 163818.5 40 166853.5 41 170347.0 42 176092.5 43 185264.0 44 195801.0 45 210602.5 46 225613.0 47 237920.5 48 250172.5 49 257808.5 50 259470.0 51 256595.0 52 247227.5 53 234141.5 54 218790.0 55 195540.5 56 171063.0 57 146240.0 58 121486.0 59 100925.5 60 80526.5 61 62245.5 62 48470.0 63 37416.0 64 27516.5 65 19485.5 66 14175.0 67 10754.0 68 8077.5 69 5988.0 70 4583.5 71 3546.0 72 2725.5 73 2056.0 74 1561.0 75 1179.0 76 869.5 77 623.0 78 453.0 79 324.5 80 217.5 81 151.0 82 115.0 83 87.5 84 70.5 85 56.5 86 44.5 87 39.5 88 39.0 89 25.5 90 15.5 91 13.5 92 16.5 93 11.5 94 8.0 95 7.0 96 2.5 97 2.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21843137500192297 2 0.14540114978651336 3 0.024191262102604423 4 0.023075522550702332 5 0.028772556323293313 6 0.05617579592379769 7 0.06273499207740393 8 0.05517839238497613 9 0.04337860136637523 10-11 0.045542459891276255 12-13 0.04365753625435075 14-15 0.020844043446898153 16-17 0.019533894730649485 18-19 0.018874594086343705 20-21 0.02212038187369524 22-23 0.025391527378135458 24-25 0.022264075603864446 26-27 0.019922713059342637 28-29 0.019440916434657642 30-31 0.017674328810812667 32-33 0.02555212625303046 34-35 0.02573808284501414 36-37 0.024985803904716518 38-39 0.023379815155766537 40-41 0.024081378661886792 42-43 0.029651623849034355 44-45 0.03940589235581475 46-47 0.04481553866806731 48-49 0.041383794288732095 50-51 0.028231591692068056 52-53 0.044173143168487326 54-55 0.043099666478399704 56-57 0.04523816728621205 58-59 0.04836561906048306 60-61 0.024647701010200734 62-63 0.025780345706828613 64-65 0.026735486383835706 66-67 0.03158726292013722 68-69 0.035568424503060594 70-71 0.04002293013830606 72-73 0.04506911583895415 74-75 0.025247833647966254 76-77 0.019077455823053174 78-79 0.018536491191827917 80-81 0.021249766920317093 82-83 0.01947472672410922 84-85 0.011951937321133003 86-87 0.0138453135304214 88-89 0.01954234730301238 90-91 0.02042141482875342 92-93 0.020725707433817627 94-95 0.024461744418217052 96-97 0.02515485535197441 98-99 0.03043771307878355 100 0.02929661580979278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5915359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.69370827064321 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0716111489771 52.73578966459619 2 13.593589658920477 18.132138061257493 3 3.6928399071340525 7.388675623697631 4 1.381654877542487 3.6859074933411966 5 0.7011791257332266 2.338211802855824 6 0.38936487054939206 1.5580912252354748 7 0.24757719641034145 1.1558288918298536 8 0.17004077720986854 0.9072519991478728 9 0.12301841091893863 0.7384098608771222 >10 0.5932873953698204 6.694154283384894 >50 0.024443598709013435 1.1138942195427315 >100 0.01006733708247736 1.2443928080424658 >500 8.069471003468441E-4 0.3716147769325342 >1k 3.746515806910375E-4 0.5233795190792404 >5k 5.763870471600534E-5 0.2396820639955739 >10k+ 8.6458057074008E-5 1.17257770618396 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26749 0.4521957162701368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21745 0.36760237206228735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12512 0.21151717080907517 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6943 0.11737241983115479 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 5.071543417736776E-5 0.0 0.0 0.0 0.0 4 5.071543417736776E-5 0.0 0.0 1.6905144725789253E-5 0.0 5 5.071543417736776E-5 0.0 0.0 1.6905144725789253E-5 0.0 6 5.071543417736776E-5 0.0 0.0 1.6905144725789253E-5 0.0 7 5.071543417736776E-5 0.0 0.0 1.6905144725789253E-5 0.0 8 5.071543417736776E-5 0.0 0.0 1.6905144725789253E-5 0.0 9 5.071543417736776E-5 0.0 0.0 5.071543417736776E-5 1.6905144725789253E-5 10-11 5.071543417736776E-5 0.0 0.0 9.297829599184089E-5 1.6905144725789253E-5 12-13 5.071543417736776E-5 0.0 0.0 2.0286173670947105E-4 8.452572362894627E-5 14-15 5.071543417736776E-5 0.0 0.0 2.1976688143526032E-4 1.0988344071763016E-4 16-17 5.071543417736776E-5 0.0 0.0 2.704823156126281E-4 1.1833601308052478E-4 18-19 5.071543417736776E-5 0.0 0.0 3.5500803924157437E-4 1.3524115780631402E-4 20-21 5.071543417736776E-5 0.0 0.0 8.875200981039359E-4 1.4369373016920865E-4 22-23 5.071543417736776E-5 0.0 0.0 0.003220430070262853 1.6905144725789254E-4 24-25 5.071543417736776E-5 0.0 0.0 0.00940771303990172 1.859565919836818E-4 26-27 5.071543417736776E-5 0.0 0.0 0.018756258073263178 2.0286173670947105E-4 28-29 5.071543417736776E-5 0.0 0.0 0.049058729994240415 2.1131430907236567E-4 30-31 5.071543417736776E-5 0.0 0.0 0.14039722694767975 2.1976688143526032E-4 32-33 5.071543417736776E-5 0.0 0.0 0.3337160094594428 2.789348879755227E-4 34-35 5.071543417736776E-5 8.452572362894627E-6 0.0 0.6307816651533744 2.8738746033841735E-4 36-37 5.071543417736776E-5 2.5357717088683878E-5 0.0 1.0777283339861536 2.8738746033841735E-4 38-39 5.071543417736776E-5 3.3810289451578506E-5 0.0 1.6887986003892579 3.042926050642066E-4 40-41 5.071543417736776E-5 3.3810289451578506E-5 0.0 2.33805758872792 3.042926050642066E-4 42-43 5.071543417736776E-5 3.3810289451578506E-5 0.0 2.947733180691147 3.2119774978999583E-4 44-45 5.071543417736776E-5 3.3810289451578506E-5 0.0 3.669320830739098 3.7191318396736356E-4 46-47 5.071543417736776E-5 3.3810289451578506E-5 0.0 4.487791865210548 4.057234734189421E-4 48-49 5.071543417736776E-5 3.3810289451578506E-5 0.0 5.316397533945108 4.7334405232209913E-4 50-51 5.071543417736776E-5 3.3810289451578506E-5 0.0 6.1343614140747835 4.7334405232209913E-4 52-53 5.071543417736776E-5 3.3810289451578506E-5 0.0 6.903942769999251 5.409646312252562E-4 54-55 5.071543417736776E-5 3.3810289451578506E-5 0.0 7.748777377670569 5.916800654026239E-4 56-57 5.071543417736776E-5 3.3810289451578506E-5 0.0 8.672533653494234 5.916800654026239E-4 58-59 5.071543417736776E-5 3.3810289451578506E-5 0.0 9.654147787141913 5.916800654026239E-4 60-61 5.071543417736776E-5 3.3810289451578506E-5 0.0 10.613430224606823 5.916800654026239E-4 62-63 5.071543417736776E-5 3.3810289451578506E-5 0.0 11.55237577296661 6.931109337573594E-4 64-65 5.071543417736776E-5 3.3810289451578506E-5 0.0 12.476791349434581 7.100160784831487E-4 66-67 5.071543417736776E-5 3.3810289451578506E-5 0.0 13.478235217845612 7.26921223208938E-4 68-69 5.071543417736776E-5 3.3810289451578506E-5 0.0 14.545541530108316 7.438263679347272E-4 70-71 5.071543417736776E-5 3.3810289451578506E-5 0.0 15.622280913128012 7.522789402976218E-4 72-73 5.071543417736776E-5 3.3810289451578506E-5 0.0 16.627824279135048 7.94541802112095E-4 74-75 5.071543417736776E-5 3.3810289451578506E-5 0.0 17.596057990732263 7.94541802112095E-4 76-77 5.071543417736776E-5 3.3810289451578506E-5 0.0 18.601677429890564 8.029943744749897E-4 78-79 5.071543417736776E-5 3.3810289451578506E-5 0.0 19.675661274319953 8.114469468378842E-4 80-81 5.071543417736776E-5 3.3810289451578506E-5 0.0 20.732866762609 8.36804663926568E-4 82-83 5.071543417736776E-5 5.071543417736776E-5 0.0 21.775195723539348 8.959726704668305E-4 84-85 5.071543417736776E-5 5.071543417736776E-5 0.0 22.765473743858994 8.959726704668305E-4 86-87 5.071543417736776E-5 6.762057890315701E-5 0.0 23.75128035339867 8.959726704668305E-4 88 5.071543417736776E-5 6.762057890315701E-5 0.0 24.51060028647458 9.128778151926198E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11105 0.0 54.771305 1 TACCTGG 5050 0.0 41.935764 2 GTATCAA 20130 0.0 40.841064 1 ACCTGGG 6205 0.0 33.863018 3 ATCAACG 24305 0.0 33.304077 3 CAACGCA 24600 0.0 32.922974 5 TATCAAC 24960 0.0 32.58086 2 TCAACGC 25640 0.0 31.660898 4 AACGCAG 26225 0.0 31.152594 6 GTACATG 19120 0.0 28.066048 1 ACGCAGA 29045 0.0 27.820938 7 TAACGCA 615 0.0 27.515429 4 CGCAGAG 29350 0.0 27.498863 8 TACATGG 19145 0.0 27.35919 2 GTGGTAT 4295 0.0 26.984686 1 ACATGGG 19675 0.0 25.731249 3 CCTGGGG 6130 0.0 25.45813 4 GCAGAGT 31510 0.0 24.986645 9 ATAACGC 705 0.0 24.670193 3 GTACTAG 2220 0.0 24.617865 1 >>END_MODULE