FastQCFastQC Report
Mon 6 Jun 2016
ERR1211383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915359
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247460.4183347113843809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207860.35139033827025545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127650.21579417242469984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59210.10009536192139817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.053.3471641
TACCTGG51600.044.6218762
GTATCAA197450.040.7159351
ACCTGGG61400.036.5064663
ATCAACG239150.032.912753
CAACGCA241400.032.816235
TATCAAC248050.032.1283232
TCAACGC250050.031.7038274
AACGCAG257850.031.2138676
ACGCAGA282900.027.951727
CCTGGGG58950.027.8205094
CGCAGAG288650.027.280978
GTACATG186800.026.8542461
TACATGG186650.026.5598642
GTGGTAT42500.026.4383531
ACATGGG186500.025.5492943
GCAGAGT312050.024.7235039
GTACCTG94650.024.6865961
TGGGGGG108200.024.1871836
CTGGGGG41650.023.6904145
Produced by FastQC (version 0.11.5)