##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5915359 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.074138864606525 33.0 31.0 34.0 30.0 34.0 2 32.1991333070402 33.0 31.0 34.0 30.0 34.0 3 32.27918660558049 34.0 31.0 34.0 30.0 34.0 4 35.921522599051045 37.0 35.0 37.0 35.0 37.0 5 35.78301993167278 37.0 35.0 37.0 35.0 37.0 6 35.82220926912466 37.0 35.0 37.0 35.0 37.0 7 35.75522804279504 37.0 35.0 37.0 33.0 37.0 8 35.743578707564495 37.0 35.0 37.0 33.0 37.0 9 37.33223342826699 39.0 37.0 39.0 34.0 39.0 10-11 37.30923643011354 39.0 37.0 39.0 34.0 39.0 12-13 37.22523611838267 39.0 37.0 39.0 33.0 39.0 14-15 38.428257777761246 40.0 38.0 41.0 33.5 41.0 16-17 38.41853672786385 40.0 38.0 41.0 34.0 41.0 18-19 38.43659353557409 40.0 38.0 41.0 34.0 41.0 20-21 38.39553956065896 40.0 38.0 41.0 34.0 41.0 22-23 38.28598272733743 40.0 38.0 41.0 34.0 41.0 24-25 38.16622718925428 40.0 37.5 41.0 33.0 41.0 26-27 38.03173543651366 40.0 37.0 41.0 33.0 41.0 28-29 37.94702510870431 40.0 37.0 41.0 33.0 41.0 30-31 37.97256328821294 40.0 37.0 41.0 33.0 41.0 32-33 37.78735119880298 40.0 37.0 41.0 33.0 41.0 34-35 37.65735553835363 40.0 37.0 41.0 33.0 41.0 36-37 37.49780013351683 40.0 36.0 41.0 32.0 41.0 38-39 37.368208759603604 40.0 36.0 41.0 32.0 41.0 40-41 37.292146174053 40.0 36.0 41.0 32.0 41.0 42-43 37.13832389547278 40.0 36.0 41.0 31.0 41.0 44-45 36.96762800026169 39.0 36.0 41.0 31.0 41.0 46-47 36.72546754981397 39.0 35.0 41.0 30.0 41.0 48-49 36.48372102183485 39.0 35.0 41.0 30.0 41.0 50-51 36.291965711633054 39.0 35.0 41.0 29.0 41.0 52-53 36.087328089470134 39.0 35.0 40.0 28.5 41.0 54-55 35.8845569643364 38.5 35.0 40.0 28.0 41.0 56-57 35.65328773790399 38.0 34.5 40.0 28.0 41.0 58-59 35.392909542768244 38.0 34.0 40.0 27.0 41.0 60-61 35.13441593316652 37.5 34.0 40.0 26.5 41.0 62-63 34.838453253640225 37.0 34.0 40.0 26.0 41.0 64-65 34.49270348595918 36.5 33.5 39.5 26.0 41.0 66-67 34.140596623129724 36.0 33.0 39.0 26.0 41.0 68-69 33.77054917546002 35.5 33.0 39.0 25.0 40.0 70-71 33.39409974948266 35.0 32.5 38.0 24.5 40.0 72-73 33.04378533914847 35.0 32.0 37.5 24.0 40.0 74-75 32.67993776878123 35.0 32.0 37.0 24.0 39.0 76-77 31.650736244410524 34.0 30.5 36.0 22.5 38.5 78-79 32.12292770396522 35.0 31.5 36.0 23.5 38.5 80-81 32.01552340272163 35.0 31.5 35.5 23.5 37.5 82-83 31.75194185171179 34.5 31.0 35.0 23.0 37.0 84-85 31.48168547335842 34.0 31.0 35.0 22.5 36.5 86-87 31.227861487358588 34.0 31.0 35.0 20.5 36.0 88-89 30.999454809082593 34.0 31.0 35.0 20.0 36.0 90-91 30.788339980717993 34.0 30.5 35.0 20.0 35.0 92-93 30.616280939161935 34.0 30.0 35.0 19.5 35.0 94-95 30.452783846255148 34.0 30.0 35.0 19.0 35.0 96-97 30.28416550880513 34.0 29.5 35.0 19.0 35.0 98-99 30.08819126615984 34.0 29.0 35.0 18.0 35.0 100 28.250568393228544 32.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.19372117541051992 1101 2 -0.19517735806073233 1101 3 -0.23589143760969478 1101 4 -0.16359984914748793 1101 5 -0.14401384452058608 1101 6 -0.17012529011520172 1101 7 -0.2686638097809606 1101 8 -0.2696822997140558 1101 9 -0.41955467638707944 1101 10-11 -0.41179315636824043 1101 12-13 -0.49951418167898254 1101 14-15 -0.5743658293808238 1101 16-17 -0.6203805755457594 1101 18-19 -0.6448793088120865 1101 20-21 -0.5715209311866616 1101 22-23 -0.6873913773980362 1101 24-25 -0.7286573929648128 1101 26-27 -0.7278811009490767 1101 28-29 -0.7827293311866725 1101 30-31 -0.7047614353164917 1101 32-33 -0.5568225100954578 1101 34-35 -0.6617853637106776 1101 36-37 -0.6202750944596573 1101 38-39 -0.6489690991056278 1101 40-41 -0.6243826239909254 1101 42-43 -0.6597504835712158 1101 44-45 -0.6606836932557272 1101 46-47 -0.6679642072686391 1101 48-49 -0.5280871438481896 1101 50-51 -0.5981312791633044 1101 52-53 -0.5953573386241402 1101 54-55 -0.6573855505230455 1101 56-57 -0.5291287583936111 1101 58-59 -0.6277722977170015 1101 60-61 -0.5585636759540478 1101 62-63 -0.6352954768120611 1101 64-65 -0.636465836415887 1101 66-67 -0.6503586745119136 1101 68-69 -0.7130865389713037 1101 70-71 -0.65646958212416 1101 72-73 -0.729352043905017 1101 74-75 -0.8098983143167722 1101 76-77 -0.7085156324733681 1101 78-79 -0.6079679504055377 1101 80-81 -0.527328132247149 1101 82-83 -0.5248337935020899 1101 84-85 -0.6149975039974578 1101 86-87 -0.7540588650612179 1101 88-89 -0.9121724752389149 1101 90-91 -0.9351859070116539 1101 92-93 -0.9688351743626882 1101 94-95 -1.0834265027600907 1101 96-97 -0.9872098678060937 1101 98-99 -1.1402291153376574 1101 100 -1.0719542705622942 1102 1 -0.2635129765957167 1102 2 -0.22702857129267429 1102 3 -0.3090506352660469 1102 4 -0.12311779158451941 1102 5 -0.16608703261857727 1102 6 -0.24611668661451347 1102 7 -0.24087437937065914 1102 8 -0.2629609078631674 1102 9 -0.35239554668373074 1102 10-11 -0.4059509911742083 1102 12-13 -0.44087345911921005 1102 14-15 -0.5230545393538648 1102 16-17 -0.6212117308633296 1102 18-19 -0.6373688938628561 1102 20-21 -0.5774893407532531 1102 22-23 -0.5799777887184163 1102 24-25 -0.6472003985561727 1102 26-27 -0.6992438992215 1102 28-29 -0.6289200134213502 1102 30-31 -0.5945686091779194 1102 32-33 -0.6246459921375873 1102 34-35 -0.6078188301615199 1102 36-37 -0.5789419829888374 1102 38-39 -0.6319751107810987 1102 40-41 -0.6847999048742892 1102 42-43 -0.6347400029701689 1102 44-45 -0.5712585147978544 1102 46-47 -0.6849783818331474 1102 48-49 -0.6101631942297914 1102 50-51 -0.6122857927997956 1102 52-53 -0.6311322966679 1102 54-55 -0.6743098768610096 1102 56-57 -0.6716423266602618 1102 58-59 -0.7530312331992732 1102 60-61 -0.7629564623495853 1102 62-63 -0.6630719799069027 1102 64-65 -0.816429769882582 1102 66-67 -0.850355378384883 1102 68-69 -0.8366391195748051 1102 70-71 -0.7885587764045212 1102 72-73 -0.8896522107553722 1102 74-75 -0.8142798789903054 1102 76-77 -0.7455159443533148 1102 78-79 -0.6815898241271867 1102 80-81 -0.5622611227291259 1102 82-83 -0.5589778793297207 1102 84-85 -0.6389419886902985 1102 86-87 -0.8249671869765507 1102 88-89 -0.7692257065398564 1102 90-91 -0.7694686704466527 1102 92-93 -0.7754988467652915 1102 94-95 -0.9945863530089873 1102 96-97 -1.030479893842113 1102 98-99 -0.9639496021411027 1102 100 -1.140608434146035 1103 1 -0.33069674051943565 1103 2 -0.2994343829699453 1103 3 -0.335182056021754 1103 4 -0.18456151186784098 1103 5 -0.214301225980968 1103 6 -0.30123318204635297 1103 7 -0.3196153935312367 1103 8 -0.3687038674655341 1103 9 -0.47943396915930947 1103 10-11 -0.5251066881386421 1103 12-13 -0.5364844824458075 1103 14-15 -0.7147263593872992 1103 16-17 -0.7194736662258023 1103 18-19 -0.6401688363138689 1103 20-21 -0.6999642139639306 1103 22-23 -0.8404553765246376 1103 24-25 -0.7230845264799797 1103 26-27 -0.8326528045693777 1103 28-29 -0.909311833432767 1103 30-31 -0.7366897762009117 1103 32-33 -0.7195791918501726 1103 34-35 -0.7621604224528227 1103 36-37 -0.6239433226808018 1103 38-39 -0.6889307942110605 1103 40-41 -0.8134491900234551 1103 42-43 -0.8654676262630971 1103 44-45 -0.7080856290628574 1103 46-47 -0.7696806125990747 1103 48-49 -0.8341428413653844 1103 50-51 -0.7768203915139154 1103 52-53 -0.8623431856401709 1103 54-55 -0.952753572936345 1103 56-57 -0.9436373453169153 1103 58-59 -1.0623758047234233 1103 60-61 -0.9033680778915496 1103 62-63 -0.8910106643789106 1103 64-65 -0.9142993570593987 1103 66-67 -0.8876321009756651 1103 68-69 -0.8224894140869665 1103 70-71 -0.8428760644278412 1103 72-73 -0.840369152274377 1103 74-75 -0.9526955735779481 1103 76-77 -0.8409861899873512 1103 78-79 -0.7591695426763359 1103 80-81 -0.6732973785899325 1103 82-83 -0.7055167975188326 1103 84-85 -0.6551543934126158 1103 86-87 -0.700070208463341 1103 88-89 -0.638892522122319 1103 90-91 -0.7896936864294268 1103 92-93 -0.938988582028955 1103 94-95 -0.9515132619462427 1103 96-97 -1.026719815892399 1103 98-99 -1.2192844371031555 1103 100 -1.2854226708627259 1104 1 -0.34502379768794356 1104 2 -0.29975820111446083 1104 3 -0.3224007530511237 1104 4 -0.23692810855177981 1104 5 -0.2274219341793895 1104 6 -0.23576149935330903 1104 7 -0.3033125331329458 1104 8 -0.2764141867184904 1104 9 -0.437274559918194 1104 10-11 -0.3999456391644429 1104 12-13 -0.5021506140027512 1104 14-15 -0.6715110481251827 1104 16-17 -0.6218765485162265 1104 18-19 -0.6236169687495945 1104 20-21 -0.680436881030424 1104 22-23 -0.7215393240616024 1104 24-25 -0.8260998231660679 1104 26-27 -0.7658685946779258 1104 28-29 -0.8483334785553254 1104 30-31 -0.7687455081682444 1104 32-33 -0.7022192358254102 1104 34-35 -0.7103335811864753 1104 36-37 -0.6470841206636351 1104 38-39 -0.7876966623182113 1104 40-41 -0.8656123120797261 1104 42-43 -0.7374983922165868 1104 44-45 -0.834318202247367 1104 46-47 -0.8274961060475192 1104 48-49 -0.7578878810569805 1104 50-51 -0.7447150196011947 1104 52-53 -0.7405967435323504 1104 54-55 -0.669791455586946 1104 56-57 -0.7472508560041149 1104 58-59 -0.8546241375725216 1104 60-61 -0.8750152085131404 1104 62-63 -0.9102868662307984 1104 64-65 -0.9284048950939123 1104 66-67 -1.0159482178372272 1104 68-69 -0.7901597145567862 1104 70-71 -0.7494361166150796 1104 72-73 -0.7718498963989262 1104 74-75 -0.7705584868655997 1104 76-77 -0.6976710160741142 1104 78-79 -0.6352756112333822 1104 80-81 -0.8229131920931501 1104 82-83 -0.7490348457463867 1104 84-85 -0.7467932081146316 1104 86-87 -0.6538890684409182 1104 88-89 -0.7851388224719322 1104 90-91 -0.5747181609586569 1104 92-93 -0.8306099771122248 1104 94-95 -0.7697148607878006 1104 96-97 -0.8085051611132918 1104 98-99 -0.7760731314405938 1104 100 -1.0584553597848476 1105 1 -0.37898808971788966 1105 2 -0.3110318155511891 1105 3 -0.3754887809161822 1105 4 -0.26666000963284375 1105 5 -0.2518229670504013 1105 6 -0.34150258262749134 1105 7 -0.30480206655584396 1105 8 -0.31502954954334683 1105 9 -0.44941808574289865 1105 10-11 -0.45627413639746095 1105 12-13 -0.4832979020458694 1105 14-15 -0.6853630854137975 1105 16-17 -0.6909716130455266 1105 18-19 -0.6511977592261289 1105 20-21 -0.75118800889976 1105 22-23 -0.712048778217266 1105 24-25 -0.6836182675905818 1105 26-27 -0.6610199173210489 1105 28-29 -0.7692083682407826 1105 30-31 -0.616434698138896 1105 32-33 -0.656500982319443 1105 34-35 -0.6950663329362214 1105 36-37 -0.7775633865575173 1105 38-39 -0.7584779281634866 1105 40-41 -0.6791358990687684 1105 42-43 -0.6813546618961581 1105 44-45 -0.7160647130391311 1105 46-47 -0.7917426608393825 1105 48-49 -0.724333133613392 1105 50-51 -0.6887839145448638 1105 52-53 -0.731805053433888 1105 54-55 -0.6797367529815546 1105 56-57 -0.7296582064519441 1105 58-59 -0.710952986806376 1105 60-61 -0.7804240197644248 1105 62-63 -0.7512291556944888 1105 64-65 -0.7309042853781875 1105 66-67 -0.8039244896287983 1105 68-69 -0.8943314272671117 1105 70-71 -0.788734009052007 1105 72-73 -0.8065996196173231 1105 74-75 -0.9790581168456782 1105 76-77 -0.9486146381123852 1105 78-79 -0.7558278502520039 1105 80-81 -0.7230397447730077 1105 82-83 -0.6628556220087916 1105 84-85 -0.6717570232672614 1105 86-87 -0.5654380313375178 1105 88-89 -0.6180778366516968 1105 90-91 -0.6666976041101016 1105 92-93 -0.6706614865672229 1105 94-95 -0.7303634809864441 1105 96-97 -0.8481073163640964 1105 98-99 -0.8804586092272437 1105 100 -0.782569076167718 1106 1 -0.3246337065267362 1106 2 -0.307265215003806 1106 3 -0.34082139000895495 1106 4 -0.20004203521082076 1106 5 -0.23430410527203804 1106 6 -0.24885954107580233 1106 7 -0.3082231655585588 1106 8 -0.37703207468565125 1106 9 -0.497038680687119 1106 10-11 -0.4300673301528306 1106 12-13 -0.5306647864696856 1106 14-15 -0.686978546009783 1106 16-17 -0.6985320378255366 1106 18-19 -0.7388719770705805 1106 20-21 -0.658959675897826 1106 22-23 -0.6406592224774172 1106 24-25 -0.6954793233328544 1106 26-27 -0.7812220095356253 1106 28-29 -0.8108532834415882 1106 30-31 -0.7103609549306782 1106 32-33 -0.674593756241606 1106 34-35 -0.6606900608674948 1106 36-37 -0.6854080272582266 1106 38-39 -0.7307166074591009 1106 40-41 -0.6054617846872574 1106 42-43 -0.572697998802191 1106 44-45 -0.7449491885871211 1106 46-47 -0.6891494754903817 1106 48-49 -0.7261090910901231 1106 50-51 -0.7595029657897285 1106 52-53 -0.7397790713038717 1106 54-55 -0.8238836283200328 1106 56-57 -0.8263094506745929 1106 58-59 -0.8002668012100358 1106 60-61 -0.9614574366764046 1106 62-63 -0.8170676662161895 1106 64-65 -0.8210614520456616 1106 66-67 -0.8753349481151034 1106 68-69 -0.8461273983556907 1106 70-71 -0.8275529472959491 1106 72-73 -0.7549964452450908 1106 74-75 -0.8419666863250796 1106 76-77 -0.7786094164672939 1106 78-79 -0.6930870362250161 1106 80-81 -0.6639521423810741 1106 82-83 -0.6362240034119786 1106 84-85 -0.5930954456838009 1106 86-87 -0.6860126386229233 1106 88-89 -0.6852889931895056 1106 90-91 -0.7635988244877758 1106 92-93 -0.839299494000386 1106 94-95 -0.7331331191409909 1106 96-97 -0.7676204909141582 1106 98-99 -0.8573765776678179 1106 100 -0.9767806805060495 1107 1 -0.36454261268201194 1107 2 -0.3289712294950178 1107 3 -0.3783280192482543 1107 4 -0.18391021152227438 1107 5 -0.16900135398591232 1107 6 -0.2936210756991571 1107 7 -0.2744909645600231 1107 8 -0.2573684225516928 1107 9 -0.5494664751139027 1107 10-11 -0.46056271650591185 1107 12-13 -0.4987322847769562 1107 14-15 -0.6064488179199188 1107 16-17 -0.6361874264355976 1107 18-19 -0.6390306043929641 1107 20-21 -0.6742176481906412 1107 22-23 -0.626401866805061 1107 24-25 -0.5878381572243612 1107 26-27 -0.6541224130589427 1107 28-29 -0.5331089681407946 1107 30-31 -0.6553618000321748 1107 32-33 -0.6877566864969538 1107 34-35 -0.6756682198012243 1107 36-37 -0.6640793037764467 1107 38-39 -0.6195230103933724 1107 40-41 -0.644282717547739 1107 42-43 -0.6208447468819926 1107 44-45 -0.7056270776450262 1107 46-47 -0.7859764103426699 1107 48-49 -0.8382329323648818 1107 50-51 -0.8714134442386339 1107 52-53 -0.8675605685403411 1107 54-55 -0.7809882751637574 1107 56-57 -0.8056193924081327 1107 58-59 -0.764878935045779 1107 60-61 -0.8185870823309145 1107 62-63 -0.8247534721545691 1107 64-65 -0.8355808777409948 1107 66-67 -0.8123477812596818 1107 68-69 -0.7780274939483078 1107 70-71 -0.7590203395353896 1107 72-73 -0.7685716787613117 1107 74-75 -0.7486047308715484 1107 76-77 -0.746419715471287 1107 78-79 -0.6325997504481258 1107 80-81 -0.5797425539202017 1107 82-83 -0.6174769511462443 1107 84-85 -0.49031506808030656 1107 86-87 -0.6177977662534921 1107 88-89 -0.6588257088012135 1107 90-91 -0.6464206310415115 1107 92-93 -0.7337578294844249 1107 94-95 -0.6925043901938857 1107 96-97 -0.7174112144323708 1107 98-99 -0.7428484352818749 1107 100 -0.7583931695880466 1108 1 -0.47021048345288463 1108 2 -0.3690400144503485 1108 3 -0.4605611815143611 1108 4 -0.2724586076162723 1108 5 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0.7754863953006037 2115 40-41 0.7293343882991579 2115 42-43 0.7866330896349112 2115 44-45 0.8960394847204682 2115 46-47 0.8686751225334746 2115 48-49 0.8518813610679885 2115 50-51 0.8977333081326151 2115 52-53 0.9069416360353557 2115 54-55 0.7857953533935316 2115 56-57 0.7307199627173304 2115 58-59 0.6864408155897408 2115 60-61 0.7509339873323526 2115 62-63 0.8602409244972193 2115 64-65 0.9139423316453659 2115 66-67 0.8846735476074983 2115 68-69 0.9057728866167238 2115 70-71 0.9079703320154664 2115 72-73 0.9014401898861308 2115 74-75 0.6321321434969676 2115 76-77 0.5936342794908605 2115 78-79 0.4421318599124291 2115 80-81 0.6043793014510364 2115 82-83 0.682128351525435 2115 84-85 0.6888511906758339 2115 86-87 0.7244416481067724 2115 88-89 0.7736361572438604 2115 90-91 0.8503739666946792 2115 92-93 0.8934364193695004 2115 94-95 0.9424385839138125 2115 96-97 0.9594816143559122 2115 98-99 1.031679224976358 2115 100 0.9445968455882898 2116 1 0.1012678820804922 2116 2 0.23748036481290313 2116 3 0.32943558507350446 2116 4 0.17924365236862627 2116 5 0.29258537768284043 2116 6 0.27156355438187774 2116 7 0.29815463132904085 2116 8 0.33941825641266377 2116 9 0.4929560329564566 2116 10-11 0.4823104553104187 2116 12-13 0.4131747907879699 2116 14-15 0.6319996552996585 2116 16-17 0.7032611950150951 2116 18-19 0.5809972712905491 2116 20-21 0.6914532622777685 2116 22-23 0.7495900463644531 2116 24-25 0.7915011026083008 2116 26-27 0.7632449200987139 2116 28-29 0.776603875261273 2116 30-31 0.507183698683157 2116 32-33 0.5932890839754137 2116 34-35 0.554993385047581 2116 36-37 0.5681142284061735 2116 38-39 0.4883167682481684 2116 40-41 0.6085552905555645 2116 42-43 0.7279621985689673 2116 44-45 0.7569200680826214 2116 46-47 0.6566665915019811 2116 48-49 0.5496172636295853 2116 50-51 0.6559934583899931 2116 52-53 0.6425055590042916 2116 54-55 0.5644097665385885 2116 56-57 0.6992697636627554 2116 58-59 0.7449372583793519 2116 60-61 0.7602737204228802 2116 62-63 0.7108483461470314 2116 64-65 0.5826614307114752 2116 66-67 0.5228494896409046 2116 68-69 0.5189959430369981 2116 70-71 0.40284391370922634 2116 72-73 0.5633620372278898 2116 74-75 0.5103189877528749 2116 76-77 0.5049810685065772 2116 78-79 0.5453852535047048 2116 80-81 0.5810083189963287 2116 82-83 0.5611668577344489 2116 84-85 0.5124265898022955 2116 86-87 0.4981294605071618 2116 88-89 0.45849489038186775 2116 90-91 0.36327141883002056 2116 92-93 0.521160766931672 2116 94-95 0.6076253120262898 2116 96-97 0.6973478688405734 2116 98-99 0.6843299443874038 2116 100 0.4462551109310908 2201 1 0.6749343300292026 2201 2 0.6508758086669317 2201 3 0.6208941559488821 2201 4 0.4139571497762873 2201 5 0.515961038318629 2201 6 0.4828392716691994 2201 7 0.5353269129517102 2201 8 0.506772898611608 2201 9 0.6958949658279749 2201 10-11 0.6101136978937305 2201 12-13 0.6561962538318085 2201 14-15 0.7950496099425806 2201 16-17 0.7560583421800047 2201 18-19 0.6800667115518664 2201 20-21 0.697388765580051 2201 22-23 0.664042238563745 2201 24-25 0.6872560694779111 2201 26-27 0.7208636104442832 2201 28-29 0.7190218470645817 2201 30-31 0.9471416089227418 2201 32-33 0.7947442754791183 2201 34-35 0.9277625515528953 2201 36-37 0.9431969423374298 2201 38-39 0.9938410100856885 2201 40-41 1.0171794784306982 2201 42-43 0.01943473064162049 2201 44-45 0.7643479404589044 2201 46-47 0.9551701886952202 2201 48-49 0.9703367784701342 2201 50-51 1.0236499806669528 2201 52-53 1.0559767473957464 2201 54-55 1.0666479279811298 2201 56-57 1.040284489476491 2201 58-59 1.0439489042140266 2201 60-61 0.9697217309858885 2201 62-63 0.9847776141948543 2201 64-65 0.9604276750014975 2201 66-67 0.991224867915399 2201 68-69 1.0261541155588532 2201 70-71 1.0661105918496219 2201 72-73 1.0168334900529317 2201 74-75 0.9781621822182274 2201 76-77 1.0433825629597457 2201 78-79 0.9081383254162958 2201 80-81 0.8271511292710478 2201 82-83 0.7790995114286261 2201 84-85 0.857284364867013 2201 86-87 0.829243322026425 2201 88-89 0.7778056464647385 2201 90-91 0.09110769320273349 2201 92-93 0.5396773706721092 2201 94-95 0.720756187830343 2201 96-97 0.6821754088372742 2201 98-99 0.6776779829176185 2201 100 0.8210853804202962 2202 1 0.634878030677811 2202 2 0.5785703342306903 2202 3 0.534409864620244 2202 4 0.34123757274964817 2202 5 0.35389172419481696 2202 6 0.4143949260575539 2202 7 0.4646425071624236 2202 8 0.4665139889304726 2202 9 0.6849764453604976 2202 10-11 0.681231737220962 2202 12-13 0.7014585040025096 2202 14-15 0.8438987186315074 2202 16-17 0.7660401328265678 2202 18-19 0.8074344843630499 2202 20-21 0.7618007843113404 2202 22-23 0.7363152544252429 2202 24-25 0.7649712792451666 2202 26-27 0.7533077811144508 2202 28-29 0.7527343783948979 2202 30-31 0.8577062727976923 2202 32-33 0.4932886455835046 2202 34-35 0.8492591750473366 2202 36-37 0.9708548683005347 2202 38-39 0.9917676139938436 2202 40-41 0.9891914213261188 2202 42-43 1.0464722118785446 2202 44-45 1.011387095312024 2202 46-47 1.0309771435075348 2202 48-49 1.049444986569405 2202 50-51 0.9676160060256578 2202 52-53 0.9872475522144839 2202 54-55 1.0190705629914731 2202 56-57 1.0247635244691153 2202 58-59 0.9957908074248891 2202 60-61 1.0180750907483116 2202 62-63 1.0523609472840434 2202 64-65 1.0585301481170362 2202 66-67 1.0647323675434919 2202 68-69 1.1093227379011452 2202 70-71 1.0866848756462844 2202 72-73 1.039979802007828 2202 74-75 0.9861059998665169 2202 76-77 1.0103789003955548 2202 78-79 0.8975751635642197 2202 80-81 0.8162893764326817 2202 82-83 0.7998489924750771 2202 84-85 0.7681884952113478 2202 86-87 0.7357269479077786 2202 88-89 0.7533596662057853 2202 90-91 0.8078551854406903 2202 92-93 0.8010687725816688 2202 94-95 0.7767359989688707 2202 96-97 0.6030119909344727 2202 98-99 0.5944471638078106 2202 100 0.9354606088916917 2203 1 0.6357261150699571 2203 2 0.6006379166622295 2203 3 0.5720124604554115 2203 4 0.37485173619020173 2203 5 0.4470860246761177 2203 6 0.45799960987952204 2203 7 0.4605279284786761 2203 8 0.4575156468731123 2203 9 0.6449835032127424 2203 10-11 0.6586977841267796 2203 12-13 0.6991044841850496 2203 14-15 0.8243890598861228 2203 16-17 0.7655072276882535 2203 18-19 0.7609250911029193 2203 20-21 0.7684580428392138 2203 22-23 0.746188613993219 2203 24-25 0.7450423798808643 2203 26-27 0.7410025042976969 2203 28-29 0.7544737846822116 2203 30-31 0.8699169981691526 2203 32-33 0.9220919666899121 2203 34-35 0.9551978169515394 2203 36-37 0.9111643299481713 2203 38-39 1.0001603396510674 2203 40-41 0.925600755312395 2203 42-43 0.9884134988457376 2203 44-45 0.9929970198456815 2203 46-47 0.9358456513848097 2203 48-49 0.9536368216972377 2203 50-51 0.9331697595653665 2203 52-53 0.9501497396591958 2203 54-55 0.8933374425746763 2203 56-57 0.9947035187398541 2203 58-59 0.9923088481856155 2203 60-61 1.0782287948271119 2203 62-63 0.9542800164680187 2203 64-65 0.960524375968653 2203 66-67 0.9409164399661947 2203 68-69 0.9517391945376588 2203 70-71 1.0078626046261618 2203 72-73 0.9588403854181067 2203 74-75 0.9727314864049603 2203 76-77 1.041325915923995 2203 78-79 0.8905459459672329 2203 80-81 0.855998209726355 2203 82-83 0.8025403930135511 2203 84-85 0.8164041382369334 2203 86-87 0.7906058048154172 2203 88-89 0.7905723902920094 2203 90-91 0.8280121946827954 2203 92-93 0.9086992944828047 2203 94-95 0.7678491537285979 2203 96-97 0.8727056246724985 2203 98-99 0.9283004387981002 2203 100 0.9707269577374618 2204 1 0.7015230412858315 2204 2 0.6220639782523989 2204 3 0.5825140830742512 2204 4 0.3717059080635394 2204 5 0.47445366826497093 2204 6 0.49039692973113347 2204 7 0.5207992011209868 2204 8 0.4799392013507955 2204 9 0.6988868870001426 2204 10-11 0.7041470282465596 2204 12-13 0.7011550853195985 2204 14-15 0.8563743839468145 2204 16-17 0.8494136086548636 2204 18-19 0.8414828922158861 2204 20-21 0.8448439473474068 2204 22-23 0.8717547155240553 2204 24-25 0.9026761348205099 2204 26-27 0.8797334129875196 2204 28-29 0.8555173522190387 2204 30-31 0.9775197341656039 2204 32-33 1.0419724099559602 2204 34-35 1.050081110795638 2204 36-37 1.10759731437075 2204 38-39 1.13065761542925 2204 40-41 1.1432732455354966 2204 42-43 1.1205182679442558 2204 44-45 1.1427722779451628 2204 46-47 1.116366383348094 2204 48-49 1.096273999657548 2204 50-51 1.0846208550483354 2204 52-53 1.1002717211297366 2204 54-55 1.0833761266640778 2204 56-57 1.1067534124656504 2204 58-59 1.140148668503791 2204 60-61 1.0444888410786106 2204 62-63 1.078591933466626 2204 64-65 1.0676468651911364 2204 66-67 1.095648446070392 2204 68-69 1.0534658071154794 2204 70-71 1.110683559935147 2204 72-73 1.0321112713126084 2204 74-75 1.0466779709324996 2204 76-77 1.017108614846638 2204 78-79 0.9603095504382466 2204 80-81 0.9187092513997079 2204 82-83 0.8530479587967505 2204 84-85 0.8940130454439839 2204 86-87 0.8012737615722649 2204 88-89 0.8789448940582609 2204 90-91 0.9315129604993153 2204 92-93 0.8643611242420945 2204 94-95 0.8834907525282354 2204 96-97 0.9258712323203362 2204 98-99 0.8596049517401916 2204 100 1.0529410261429568 2205 1 0.6335733054171442 2205 2 0.4364182337384861 2205 3 0.49332578550419726 2205 4 0.3358018162952874 2205 5 0.45730816517473727 2205 6 0.4880270433811873 2205 7 0.4922351834194103 2205 8 0.5322687662007368 2205 9 0.6793631508244786 2205 10-11 0.6713050830222613 2205 12-13 0.6977581444477821 2205 14-15 0.8608522270637451 2205 16-17 0.8239032779680286 2205 18-19 0.8198357916507106 2205 20-21 0.8292078949724981 2205 22-23 0.8733847878464971 2205 24-25 0.8899928416372305 2205 26-27 0.8430715729898353 2205 28-29 0.9526680970591599 2205 30-31 1.0413217064676203 2205 32-33 1.0862801078026934 2205 34-35 1.0691211874881503 2205 36-37 1.0366864951337078 2205 38-39 1.1145278198123805 2205 40-41 1.0938266882390408 2205 42-43 1.0642821376243745 2205 44-45 1.1338827565090739 2205 46-47 1.1392014499600052 2205 48-49 1.0979322929694106 2205 50-51 1.0835514803216881 2205 52-53 1.1250670524080562 2205 54-55 1.1350846041590756 2205 56-57 1.1402701574156708 2205 58-59 1.1712610347834485 2205 60-61 1.1788199837773803 2205 62-63 1.1243870872527424 2205 64-65 1.0713654260840073 2205 66-67 1.0699741133106784 2205 68-69 1.1846547648446375 2205 70-71 1.1881187741534376 2205 72-73 1.1314432888394848 2205 74-75 1.07343564228173 2205 76-77 1.1249830198484752 2205 78-79 1.0260086680394167 2205 80-81 0.9488694130719146 2205 82-83 0.9748352890297838 2205 84-85 0.9462777764620824 2205 86-87 0.9898959519595856 2205 88-89 1.0675966589596904 2205 90-91 1.1147707942263878 2205 92-93 1.1139065880981605 2205 94-95 1.1387214260682832 2205 96-97 1.1400217778313824 2205 98-99 1.1405644599667752 2205 100 1.3992428854256609 2206 1 0.6482614278616126 2206 2 0.6298847332276267 2206 3 0.5979064895235666 2206 4 0.2724970169847296 2206 5 0.4453988928687309 2206 6 0.48095289589685564 2206 7 0.5007915363978768 2206 8 0.5239730356285364 2206 9 0.7131788582026815 2206 10-11 0.7157577150604979 2206 12-13 0.7216486387399854 2206 14-15 0.8971835597153444 2206 16-17 0.8537674451225215 2206 18-19 0.8264230800015966 2206 20-21 0.8660437502545264 2206 22-23 0.8373017851361695 2206 24-25 0.9280792171341616 2206 26-27 0.9318316309393992 2206 28-29 0.95006992400738 2206 30-31 1.042498793047102 2206 32-33 1.0556539407106627 2206 34-35 1.0986261821049368 2206 36-37 1.1339215321583822 2206 38-39 1.0772878746611383 2206 40-41 1.0947432299323125 2206 42-43 1.111520430491133 2206 44-45 1.135575650423327 2206 46-47 1.1354159576822198 2206 48-49 1.1659353831400594 2206 50-51 1.2018240523328316 2206 52-53 1.1627857503104337 2206 54-55 1.1361874376054715 2206 56-57 1.0913199449996611 2206 58-59 1.205816183764803 2206 60-61 1.2206885071156748 2206 62-63 1.2441745892931877 2206 64-65 1.2185064091401117 2206 66-67 1.223405693339835 2206 68-69 1.1915745366876322 2206 70-71 1.2393154068596743 2206 72-73 1.1624320947228597 2206 74-75 1.179779993871911 2206 76-77 1.1875761629564643 2206 78-79 1.0665042891533076 2206 80-81 1.0341427188656986 2206 82-83 1.0363949791356362 2206 84-85 1.0455569396708029 2206 86-87 1.123323527524473 2206 88-89 1.1429836556580035 2206 90-91 1.1375653543565853 2206 92-93 1.1868776170837911 2206 94-95 1.1920382874985371 2206 96-97 1.1674044506828203 2206 98-99 1.1783328814437262 2206 100 1.3547559230798072 2207 1 0.7040596654365601 2207 2 0.6386472375026244 2207 3 0.597454032258085 2207 4 0.4212794935410997 2207 5 0.5126960915655445 2207 6 0.5030704206771901 2207 7 0.5283065327252103 2207 8 0.552342869072227 2207 9 0.702338010811971 2207 10-11 0.701724334681785 2207 12-13 0.7624708929343669 2207 14-15 0.9302065174061198 2207 16-17 0.9038785054824316 2207 18-19 0.8363128477447646 2207 20-21 0.8607081256186575 2207 22-23 0.8527100774474263 2207 24-25 0.8747754607738258 2207 26-27 0.9394199567725252 2207 28-29 0.8827402206345596 2207 30-31 0.9550137779635648 2207 32-33 1.035178016177582 2207 34-35 1.0324351856719716 2207 36-37 1.0266135876830234 2207 38-39 1.0709839888689459 2207 40-41 1.0641396373342502 2207 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2311 20-21 0.8883361375420336 2311 22-23 0.8260698560896103 2311 24-25 0.7476304366463538 2311 26-27 0.7651484084708073 2311 28-29 0.7668900274811463 2311 30-31 0.8730708551313597 2311 32-33 0.992558005751313 2311 34-35 0.9766861968869023 2311 36-37 0.9952300317888358 2311 38-39 1.0099666625399237 2311 40-41 1.019762003029136 2311 42-43 1.1180635199220248 2311 44-45 1.0866946452496435 2311 46-47 1.161970872685913 2311 48-49 1.17663634396785 2311 50-51 1.1967621158952753 2311 52-53 1.1654411826829403 2311 54-55 1.1104118278493758 2311 56-57 1.1870213492864323 2311 58-59 1.1926738334322025 2311 60-61 1.201141426553967 2311 62-63 1.2007800586206088 2311 64-65 1.2501333123182334 2311 66-67 1.2867499653280063 2311 68-69 1.2719287125930236 2311 70-71 1.2741086071553198 2311 72-73 1.268599722217317 2311 74-75 1.1559088926154502 2311 76-77 1.098193753496009 2311 78-79 1.0088658560417016 2311 80-81 1.0303266065227525 2311 82-83 1.0289702932524136 2311 84-85 1.061521462952399 2311 86-87 1.17292116240991 2311 88-89 1.1822581567666468 2311 90-91 1.2149589981112037 2311 92-93 1.2374869297223725 2311 94-95 1.2826517390875978 2311 96-97 1.314273925923029 2311 98-99 1.3432132953387743 2311 100 1.4270361891551921 2312 1 0.10594972151019988 2312 2 0.1533862021390746 2312 3 0.3328533174229591 2312 4 0.26143346355078734 2312 5 0.2951302866128742 2312 6 0.3986535308422532 2312 7 0.4616350316696085 2312 8 0.2965846949174846 2312 9 0.3698203170012704 2312 10-11 0.24043585181119198 2312 12-13 0.45681825728991754 2312 14-15 0.7903630566085269 2312 16-17 0.8501105268069367 2312 18-19 0.8735956973911527 2312 20-21 0.8673059476314435 2312 22-23 0.5047834761379022 2312 24-25 0.4937098727881022 2312 26-27 0.8404207702798487 2312 28-29 0.9190024336679272 2312 30-31 0.844073661370345 2312 32-33 0.7338410965298792 2312 34-35 0.8565946221300038 2312 36-37 0.9273878076812849 2312 38-39 0.9121026411341973 2312 40-41 0.927250980383441 2312 42-43 0.8420193510255984 2312 44-45 0.8526266429002334 2312 46-47 0.977350315353803 2312 48-49 0.9990278860940691 2312 50-51 1.0288812048465346 2312 52-53 1.0582125198884427 2312 54-55 1.0357678547370597 2312 56-57 0.9899421872424483 2312 58-59 1.0675916432605703 2312 60-61 1.0902933680797773 2312 62-63 1.0722598471737612 2312 64-65 1.1903748316029734 2312 66-67 1.13729771707375 2312 68-69 1.152948930566005 2312 70-71 0.9684489321569458 2312 72-73 1.080784229167655 2312 74-75 1.0904596724888407 2312 76-77 0.9992764657586157 2312 78-79 0.929693161399662 2312 80-81 0.8881763226357897 2312 82-83 0.8987079164332528 2312 84-85 0.730665886368179 2312 86-87 0.9255838524836584 2312 88-89 0.6002275883401005 2312 90-91 0.9398707255797198 2312 92-93 1.1286811867216535 2312 94-95 1.1666757552724185 2312 96-97 1.2035599404110506 2312 98-99 1.2307776626297766 2312 100 1.4250363718870354 2313 1 -0.296013384892305 2313 2 9.083137587424517E-4 2313 3 0.2380136352389144 2313 4 0.06342603687133419 2313 5 0.20889057526673582 2313 6 0.33122570319933686 2313 7 0.13807567183332736 2313 8 0.09833401374902451 2313 9 0.20012318103328397 2313 10-11 0.3451030559950894 2313 12-13 0.5719578517336785 2313 14-15 0.6138492063949741 2313 16-17 0.7804545429141712 2313 18-19 0.6713396999842374 2313 20-21 0.8027651666869886 2313 22-23 0.8831283904780776 2313 24-25 0.7594559014858291 2313 26-27 0.9139597318961421 2313 28-29 0.8225288616830397 2313 30-31 0.8373258895519982 2313 32-33 0.9925768438134668 2313 34-35 0.9917790875772425 2313 36-37 0.8270551363375063 2313 38-39 0.6192874886352797 2313 40-41 0.7427792841202958 2313 42-43 0.7306481689601796 2313 44-45 0.9831892730563752 2313 46-47 1.058962907685597 2313 48-49 1.0296627610044595 2313 50-51 0.992790733279314 2313 52-53 0.9241404814026168 2313 54-55 1.0370531712193838 2313 56-57 1.109841654246864 2313 58-59 0.9852781955244652 2313 60-61 1.0443893967012983 2313 62-63 1.0838307438165202 2313 64-65 1.0745708597672419 2313 66-67 1.1427810532775666 2313 68-69 1.1566076548821584 2313 70-71 1.035900333334304 2313 72-73 1.0978509092468443 2313 74-75 0.7549145929307031 2313 76-77 0.9838944805113847 2313 78-79 0.7921116129740824 2313 80-81 0.88626813647193 2313 82-83 0.8686687813177798 2313 84-85 0.9426447982158663 2313 86-87 1.0800630526498871 2313 88-89 1.07912038413588 2313 90-91 1.1108258449232729 2313 92-93 0.8721207584555621 2313 94-95 0.9031175436298362 2313 96-97 1.2129082693573707 2313 98-99 1.3309042687673305 2313 100 1.466044668856668 2314 1 -0.3990467074249011 2314 2 -0.25836890327320816 2314 3 0.054338514211984545 2314 4 -0.2217249142203741 2314 5 0.13417508244571508 2314 6 0.17420866842005722 2314 7 0.09015832385270528 2314 8 0.14670942533285114 2314 9 0.17884434358980883 2314 10-11 0.34996339804252585 2314 12-13 0.4794800591838566 2314 14-15 0.5109320344152337 2314 16-17 0.6769420467011926 2314 18-19 0.7758114360980457 2314 20-21 0.3808439711566294 2314 22-23 0.6466605165556771 2314 24-25 0.8056180543566924 2314 26-27 0.7660135919765452 2314 28-29 0.7127818125559244 2314 30-31 0.7335707327512537 2314 32-33 0.7373766796663119 2314 34-35 0.8454017623473717 2314 36-37 0.7311142782494784 2314 38-39 0.7550670837851072 2314 40-41 0.7887687455285217 2314 42-43 0.577301991072531 2314 44-45 0.6563408250582157 2314 46-47 0.7514052292886433 2314 48-49 0.6584639864118529 2314 50-51 0.7521147535084864 2314 52-53 0.8321530844696952 2314 54-55 0.8475397549881336 2314 56-57 0.6823727605222132 2314 58-59 0.6179443501338753 2314 60-61 0.673852014364904 2314 62-63 0.42946383101939034 2314 64-65 0.6324886680578601 2314 66-67 0.7589727331793981 2314 68-69 0.8492619497398906 2314 70-71 0.8945620988527949 2314 72-73 0.8666323242758764 2314 74-75 0.9547368571123442 2314 76-77 0.7297313576801194 2314 78-79 0.7073249385774218 2314 80-81 0.7065945133077669 2314 82-83 0.672381096194691 2314 84-85 0.597056779272755 2314 86-87 0.7676447714634627 2314 88-89 0.8188059352457877 2314 90-91 0.9220124408566441 2314 92-93 0.9668062438785299 2314 94-95 0.970930301958763 2314 96-97 1.0430926410607988 2314 98-99 1.0742072948492556 2314 100 1.0844023529899403 2315 1 -0.13556298297408276 2315 2 0.11086208348054782 2315 3 0.1166309872615301 2315 4 -0.06431970011957588 2315 5 0.2790553212466733 2315 6 0.36841537409468117 2315 7 0.4101375140729431 2315 8 0.377841289824417 2315 9 0.5703583463250155 2315 10-11 0.5815475145234004 2315 12-13 0.6665853863887818 2315 14-15 0.7442560011543691 2315 16-17 0.733793650631064 2315 18-19 0.7689773081218974 2315 20-21 0.7793516257575206 2315 22-23 0.8554956764825903 2315 24-25 0.8310935553960448 2315 26-27 0.8748594090235997 2315 28-29 0.8575779615481878 2315 30-31 0.8454834386782721 2315 32-33 0.8850335271342971 2315 34-35 0.8868160225062809 2315 36-37 0.8977320067624532 2315 38-39 0.8986092978482318 2315 40-41 0.8844555454181986 2315 42-43 0.861807306041932 2315 44-45 0.7939079725525247 2315 46-47 0.8189875368398987 2315 48-49 0.870286165566931 2315 50-51 0.8092469342278648 2315 52-53 0.8551108947287673 2315 54-55 0.7484743033506618 2315 56-57 0.8559860208779639 2315 58-59 0.8280006237231774 2315 60-61 0.8951075856574349 2315 62-63 0.9632843251666685 2315 64-65 0.9610906669591728 2315 66-67 1.0598888969326126 2315 68-69 0.9117556586707138 2315 70-71 0.6289678161607952 2315 72-73 0.8822334023017433 2315 74-75 0.9964773030883336 2315 76-77 0.9295237298767312 2315 78-79 0.8220498552376441 2315 80-81 0.8164972985173229 2315 82-83 0.758059935948026 2315 84-85 0.7192618016628884 2315 86-87 0.7318444851899493 2315 88-89 0.8343491060605821 2315 90-91 0.9324988845376581 2315 92-93 0.9816006942331512 2315 94-95 1.1010282906731028 2315 96-97 1.112300198600046 2315 98-99 0.9976798212948559 2315 100 0.9871150071862509 2316 1 0.4470744109444169 2316 2 0.43856412370723774 2316 3 0.44048459960879427 2316 4 0.26683199498741317 2316 5 0.3932796641660161 2316 6 0.40181785778070633 2316 7 0.44775380573002366 2316 8 0.4322859315040546 2316 9 0.4483660972871846 2316 10-11 0.4492653000580802 2316 12-13 0.5925953657278242 2316 14-15 0.7465048345158323 2316 16-17 0.7265147963984546 2316 18-19 0.7730433420062965 2316 20-21 0.7937003524977655 2316 22-23 0.8405473623827362 2316 24-25 0.87350975385759 2316 26-27 0.9486898329094373 2316 28-29 0.8526651680589907 2316 30-31 0.7071224775687099 2316 32-33 0.648087698045515 2316 34-35 0.5765712098488649 2316 36-37 0.5272652081842821 2316 38-39 0.4544551946442681 2316 40-41 0.536686157370454 2316 42-43 0.7325563451296091 2316 44-45 0.7027009571633798 2316 46-47 0.6222688050332223 2316 48-49 0.3561996797641669 2316 50-51 0.5915539154168812 2316 52-53 0.7329137571672817 2316 54-55 0.8480267660452228 2316 56-57 0.8492979964579703 2316 58-59 0.8103976972251203 2316 60-61 0.8465688908929039 2316 62-63 0.8281009067213958 2316 64-65 0.8750454640045433 2316 66-67 0.7149647407165531 2316 68-69 0.741865548370555 2316 70-71 0.6650544975233856 2316 72-73 0.7002695189586703 2316 74-75 0.8063461028497656 2316 76-77 0.6138720362547154 2316 78-79 0.5486874993935302 2316 80-81 0.581932332330215 2316 82-83 0.4859408852448013 2316 84-85 0.5335499166587709 2316 86-87 0.538971772937284 2316 88-89 0.4768263141637341 2316 90-91 0.6333926816889566 2316 92-93 0.6608671198312024 2316 94-95 0.5339448047490336 2316 96-97 0.7099540822180366 2316 98-99 0.8334708364476207 2316 100 0.4661560078129092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 10.0 12 39.0 13 179.0 14 833.0 15 2591.0 16 6320.0 17 12642.0 18 20016.0 19 27794.0 20 36791.0 21 47353.0 22 54654.0 23 55979.0 24 55111.0 25 58816.0 26 66489.0 27 79447.0 28 96836.0 29 117319.0 30 142014.0 31 175832.0 32 219903.0 33 283493.0 34 393706.0 35 545437.0 36 816930.0 37 1261836.0 38 1161104.0 39 175863.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57968499462137 19.471320708762104 12.97115495206587 23.977839344550663 2 16.035172167910687 21.273772225827713 39.42276369025109 23.268291916010508 3 18.440148772035645 26.708471962563895 30.875776094062928 23.975603171337532 4 12.494156382886812 17.39420580319154 38.38747108202063 31.724166731901015 5 12.510116029826628 37.739693061016375 34.829911225762736 14.920279683394257 6 29.368530295456285 37.499904908560914 18.84847563774236 14.28308915824044 7 26.006800275013358 33.388173715532254 22.933946923503584 17.6710790859508 8 25.770975192774436 34.88572832670621 20.940562433256083 18.402734047263273 9 26.856846962306953 15.622286136344002 20.709020732756077 36.811846168592965 10-11 24.542801340993222 26.04002456673074 29.03100543893083 20.386168653345205 12-13 25.150551375791704 24.36295499184789 28.556070196816258 21.930423435544146 14-15 22.647781245306177 25.787592007345626 26.756654480587944 24.807972266760252 16-17 21.67189346096505 28.153408596241004 27.58577552345566 22.58892241933829 18-19 21.224950167859635 27.436415947028742 29.79700809367614 21.541625791435482 20-21 22.218131176506983 26.915902638521626 29.49498576417522 21.370980420796172 22-23 22.0914149082076 26.766524229552257 29.249771653757616 21.89228920848253 24-25 21.65121661790796 26.645026370782404 29.701741409029907 22.00201560227973 26-27 21.67845786523336 26.8791654808411 29.399071924381253 22.043304729544285 28-29 21.38951229595599 27.546699460728817 29.392912293437096 21.6708759498781 30-31 22.251939839491055 26.873811663938437 29.489549911931906 21.384698584638603 32-33 22.08273029764038 26.999587455955854 29.14745406220623 21.770228184197535 34-35 22.13095592684519 27.134708493973953 29.376931819613333 21.357403759567525 36-37 21.942174685042698 26.89278768918576 29.268411262365774 21.896626363405765 38-39 21.75888898996536 26.936266362129647 29.428676890570593 21.876167757334404 40-41 21.84587740758175 26.380833457093278 29.85945293680449 21.913836198520475 42-43 21.500561557419722 26.711334297114124 29.29760058141289 22.490503564053256 44-45 21.113007115398077 27.13195660090708 29.571887567039713 22.18314871665513 46-47 21.116148810824082 26.626464447809422 29.26662976311164 22.990756978254858 48-49 21.238165833153495 26.693706288740866 29.266071385853326 22.802056492252312 50-51 21.15819870510056 26.93939468227286 29.044143069565976 22.858263543060605 52-53 20.889787973743445 27.167025479637946 28.818078019291534 23.125108527327075 54-55 20.707630894377534 26.88067693490128 29.074841940404024 23.336850230317165 56-57 21.10605443292416 26.713416520573375 28.58898391762198 23.59154512888049 58-59 20.6673779237599 26.834232210478675 28.84866039959056 23.649729466170864 60-61 21.118546202686524 26.976269458650854 28.395681175872433 23.50950316279019 62-63 20.979264805449592 26.747851547806917 28.31226792194147 23.960615724802018 64-65 20.55470901059748 26.50510564678176 28.30641185099817 24.633773491622588 66-67 20.67419983229314 26.521598092782682 28.012575646515348 24.791626428408826 68-69 20.459223077305605 26.59661703619733 28.05881469408301 24.885345192414057 70-71 20.523101671235107 26.280453890484427 28.071367979293306 25.125076458987156 72-73 20.454521047406203 26.39116062127708 27.82444700398676 25.329871327329954 74-75 20.619891897449357 26.40333946378916 27.55785603597376 25.418912602787724 76-77 20.512576834949652 26.290548138628427 27.590658899326137 25.606216127095788 78-79 20.39816156909104 26.27158447836535 27.614200702024426 25.716053250519185 80-81 20.244538251259883 26.317522733689135 27.445898130757385 25.992040884293594 82-83 20.493804948043206 25.890770965069905 27.753433693882428 25.861990393004465 84-85 20.68213078979671 25.974323055254523 27.282001149259553 26.06154500568921 86-87 20.320370146267884 26.12629891702686 27.43875300244475 26.11457793426051 88-89 20.334071600497072 25.63442217802261 27.505556033412066 26.525950188068254 90-91 20.573945991998727 25.24507006144952 27.517021349636895 26.663962596914857 92-93 20.446792182020346 25.234281263662478 27.575271884126195 26.74365467019098 94-95 20.015286788314583 25.027246815104505 27.890121432924232 27.06734496365668 96-97 20.07522232173159 24.92974586790744 27.888589139803717 27.10644267055725 98-99 20.044966250371367 25.04662249232371 27.706019505271485 27.20239175203344 100 19.986361645009477 24.94418943685227 27.820688603350273 27.24876031478798 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3246.0 1 2247.5 2 2685.5 3 3919.0 4 2717.0 5 1887.5 6 2624.0 7 4305.5 8 6281.5 9 7080.0 10 5946.0 11 4802.0 12 4875.5 13 5423.0 14 6041.0 15 6722.0 16 7507.5 17 8248.5 18 9098.5 19 9966.0 20 11124.5 21 12686.0 22 14377.5 23 16767.5 24 19831.0 25 23701.0 26 28989.5 27 34979.0 28 42039.0 29 51534.5 30 63160.0 31 74087.0 32 86788.0 33 99413.0 34 110322.5 35 121535.5 36 132990.0 37 147404.0 38 157012.5 39 160286.5 40 162549.5 41 165904.0 42 172589.0 43 182013.0 44 193733.5 45 209512.0 46 223658.5 47 234347.5 48 246682.5 49 255783.0 50 259773.0 51 261581.0 52 257527.0 53 246747.0 54 230537.0 55 205537.0 56 180219.5 57 154389.5 58 127415.5 59 104965.0 60 82892.5 61 63157.0 62 48311.5 63 36791.0 64 26651.0 65 18328.0 66 12962.5 67 9829.5 68 7422.5 69 5350.0 70 3991.0 71 3041.0 72 2369.0 73 1834.5 74 1297.5 75 885.5 76 619.5 77 402.0 78 249.0 79 167.0 80 125.5 81 77.5 82 38.5 83 19.5 84 15.5 85 14.0 86 9.0 87 5.0 88 2.5 89 1.5 90 3.0 91 2.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052050940610705115 2 0.0 3 0.0 4 0.013287443754470354 5 0.0040910450236409995 6 0.0 7 1.6905144725789256E-4 8 4.3953376287052064E-4 9 0.0021976688143526034 10-11 7.100160784831486E-4 12-13 3.296503221528905E-4 14-15 4.226286181447314E-5 16-17 1.0143086835473553E-4 18-19 0.0 20-21 1.6905144725789256E-5 22-23 0.0 24-25 9.29782959918409E-5 26-27 8.452572362894628E-6 28-29 0.0010227612559102499 30-31 8.452572362894627E-4 32-33 0.014183416424937185 34-35 0.023793991201548376 36-37 0.03142666404524222 38-39 0.021046905183607623 40-41 1.6059887489499792E-4 42-43 0.0019187339263770805 44-45 0.009821889085683556 46-47 0.014251037003840342 48-49 0.005671676055502295 50-51 0.010379758861634602 52-53 0.016059887489499793 54-55 0.009813436513320662 56-57 0.0031950723531741694 58-59 0.008106016896015948 60-61 0.0015468207424097166 62-63 9.128778151926198E-4 64-65 0.01898447752706133 66-67 0.044984590115325206 68-69 0.05519529752970191 70-71 0.048610743659007 72-73 0.040732946216789204 74-75 0.02899232320472857 76-77 0.02889089233637384 78-79 0.01267885854434194 80-81 0.002180763669626814 82-83 0.0278512259357378 84-85 0.048289545909217 86-87 0.048779795106264894 88-89 0.059886475191108436 90-91 0.03465554668786797 92-93 0.026718581239109918 94-95 0.007565052264790691 96-97 0.0038290152803912665 98-99 0.0231262379848797 100 0.01954234730301238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5915359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.02020666275664 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4431845453069 51.7883525496592 2 14.041968343117725 18.541073039290374 3 3.7215910041853615 7.371006216317208 4 1.4733680348779532 3.890882486117684 5 0.7025511904771379 2.3191287393233178 6 0.4072120972712519 1.6130536090453569 7 0.2505121811257359 1.1577206178621307 8 0.1852144235198716 0.9782315614164208 9 0.12949250413470265 0.7694209695825843 >10 0.6068115306324806 6.798737695884414 >50 0.025517838030851007 1.1302684805235932 >100 0.011234396586965165 1.35559622893772 >500 7.293011823726173E-4 0.3412576621766779 >1k 4.6675013394453145E-4 0.5997550349643752 >5k 5.834376673921098E-5 0.2227534996997767 >10k+ 8.751565010881646E-5 1.1227616091991945 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24746 0.4183347113843809 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20786 0.35139033827025545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12765 0.21579417242469984 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5921 0.10009536192139817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.6905144725789253E-5 2 0.0 0.0 0.0 0.0 1.6905144725789253E-5 3 0.0 0.0 0.0 0.0 1.6905144725789253E-5 4 0.0 0.0 0.0 1.6905144725789253E-5 1.6905144725789253E-5 5 0.0 0.0 0.0 1.6905144725789253E-5 1.6905144725789253E-5 6 0.0 0.0 0.0 1.6905144725789253E-5 6.762057890315701E-5 7 0.0 0.0 0.0 1.6905144725789253E-5 6.762057890315701E-5 8 0.0 0.0 0.0 1.6905144725789253E-5 8.452572362894627E-5 9 0.0 0.0 0.0 3.3810289451578506E-5 1.0143086835473553E-4 10-11 0.0 0.0 0.0 6.762057890315701E-5 1.1833601308052478E-4 12-13 0.0 0.0 0.0 1.6905144725789254E-4 1.859565919836818E-4 14-15 0.0 0.0 0.0 2.1976688143526032E-4 2.1976688143526032E-4 16-17 0.0 0.0 0.0 2.704823156126281E-4 2.3667202616104956E-4 18-19 0.0 0.0 0.0 3.5500803924157437E-4 2.3667202616104956E-4 20-21 0.0 0.0 0.0 9.044252428297252E-4 2.7048231561262805E-4 22-23 0.0 0.0 0.0 0.0032542403597144317 2.789348879755227E-4 24-25 0.0 0.0 0.0 0.009475333618804878 3.1274517742710124E-4 26-27 0.0 0.0 0.0 0.018874594086343705 3.5500803924157437E-4 28-29 0.0 0.0 0.0 0.049286949448038575 3.5500803924157437E-4 30-31 0.0 0.0 0.0 0.14093819157890503 3.719131839673636E-4 32-33 0.0 0.0 0.0 0.3345105512615549 3.8881832869315286E-4 34-35 0.0 0.0 0.0 0.6324721796259534 4.141760457818367E-4 36-37 0.0 8.452572362894627E-6 0.0 1.0809572166287795 4.2262861814473134E-4 38-39 0.0 1.6905144725789253E-5 0.0 1.6941237209778817 4.31081190507626E-4 40-41 0.0 1.6905144725789253E-5 0.0 2.3459184810254117 4.3953376287052064E-4 42-43 0.0 1.6905144725789253E-5 0.0 2.956312541639485 4.3953376287052064E-4 44-45 0.0 1.6905144725789253E-5 0.0 3.6796836844560072 4.3953376287052064E-4 46-47 0.0 1.6905144725789253E-5 0.0 4.500817279221768 4.817966246849938E-4 48-49 0.0 1.6905144725789253E-5 0.0 5.328848173035652 5.578697759510454E-4 50-51 0.0 1.6905144725789253E-5 0.0 6.147437543520182 5.578697759510454E-4 52-53 0.0 1.6905144725789253E-5 0.0 6.917965587549293 6.423954995799918E-4 54-55 0.0 1.6905144725789253E-5 0.0 7.763459495864917 6.59300644305781E-4 56-57 0.0 1.6905144725789253E-5 0.0 8.688010313490695 6.762057890315702E-4 58-59 0.0 1.6905144725789253E-5 0.0 9.669979455177614 6.931109337573594E-4 60-61 0.0 1.6905144725789253E-5 0.0 10.629836667563204 6.931109337573594E-4 62-63 0.0 1.6905144725789253E-5 0.0 11.567683381515813 6.931109337573594E-4 64-65 0.0 2.5357717088683878E-5 0.0 12.490137961195593 6.931109337573594E-4 66-67 8.452572362894627E-6 3.3810289451578506E-5 0.0 13.490440732337632 6.931109337573594E-4 68-69 1.6905144725789253E-5 3.3810289451578506E-5 0.0 14.556267844436828 6.931109337573594E-4 70-71 1.6905144725789253E-5 3.3810289451578506E-5 0.0 15.631654815878463 7.100160784831487E-4 72-73 1.6905144725789253E-5 3.3810289451578506E-5 0.0 16.63832237400976 8.114469468378843E-4 74-75 1.6905144725789253E-5 3.3810289451578506E-5 0.0 17.607223838823643 8.283520915636735E-4 76-77 1.6905144725789253E-5 3.3810289451578506E-5 0.0 18.6142210472771 8.283520915636735E-4 78-79 1.6905144725789253E-5 3.3810289451578506E-5 0.0 19.68867823575881 8.452572362894627E-4 80-81 1.6905144725789253E-5 3.3810289451578506E-5 0.0 20.745106087390468 8.62162381015252E-4 82-83 1.6905144725789253E-5 3.3810289451578506E-5 0.0 21.78716456600521 9.213303875555143E-4 84-85 1.6905144725789253E-5 3.3810289451578506E-5 0.0 22.77748484918667 9.29782959918409E-4 86-87 1.6905144725789253E-5 3.3810289451578506E-5 0.0 23.763240743292165 9.382355322813036E-4 88 1.6905144725789253E-5 3.3810289451578506E-5 0.0 24.52189292315141 9.635932493699876E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10875 0.0 53.347164 1 TACCTGG 5160 0.0 44.621876 2 GTATCAA 19745 0.0 40.715935 1 ACCTGGG 6140 0.0 36.506466 3 ATCAACG 23915 0.0 32.91275 3 CAACGCA 24140 0.0 32.81623 5 TATCAAC 24805 0.0 32.128323 2 TCAACGC 25005 0.0 31.703827 4 AACGCAG 25785 0.0 31.213867 6 ACGCAGA 28290 0.0 27.95172 7 CCTGGGG 5895 0.0 27.820509 4 CGCAGAG 28865 0.0 27.28097 8 GTACATG 18680 0.0 26.854246 1 TACATGG 18665 0.0 26.559864 2 GTGGTAT 4250 0.0 26.438353 1 ACATGGG 18650 0.0 25.549294 3 GCAGAGT 31205 0.0 24.723503 9 GTACCTG 9465 0.0 24.686596 1 TGGGGGG 10820 0.0 24.187183 6 CTGGGGG 4165 0.0 23.690414 5 >>END_MODULE