FastQCFastQC Report
Mon 6 Jun 2016
ERR1211382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822584
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278350.40798325092076554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220310.32291284357950006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128510.1883597182533773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119200.055.0268861
GTATCAA217550.042.149821
TACCTGG53150.040.289962
ATCAACG260550.034.5494043
CAACGCA262100.034.309225
TATCAAC269600.033.726862
TCAACGC273700.033.0089654
AACGCAG279850.032.536946
ACCTGGG67900.030.3226553
ACGCAGA307000.029.307317
GTACATG233700.028.9136181
CGCAGAG313850.028.5170448
TACATGG234150.028.32082
GTGGTAT49100.026.9672571
ACATGGG236700.026.9292533
CATGGGG178250.026.5554264
GCAGAGT335000.025.9729529
ATGGGGG140200.025.4146445
GTACTAG22900.024.2805461
TGGGGGG156900.023.2793316
Produced by FastQC (version 0.11.5)