FastQCFastQC Report
Mon 6 Jun 2016
ERR1211382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822584
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256670.3762064343949448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219740.3220773829974098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132300.19391479826411812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119100.054.794621
GTATCAA212200.040.591991
TACCTGG52050.038.462912
ATCAACG259050.032.708863
CAACGCA263350.032.188155
TATCAAC270800.031.6019972
ACCTGGG62850.031.031023
TCAACGC274550.030.9487384
AACGCAG280100.030.6817866
GTGGTAT47550.028.1808821
GTACATG232200.027.842021
ACGCAGA314350.027.0847437
TACATGG230950.027.0840342
CGCAGAG318000.026.7443168
ACATGGG232400.025.8837453
TGGTATC53250.024.3580632
CATGGGG173850.024.1673374
ATGGGGG131200.024.1389775
GCAGAGT348150.023.9558739
TGGGGGG135300.023.6151756
Produced by FastQC (version 0.11.5)