##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6822584 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09556012795152 33.0 31.0 34.0 30.0 34.0 2 32.2333991050898 33.0 31.0 34.0 30.0 34.0 3 32.30888985756716 34.0 31.0 34.0 30.0 34.0 4 35.94641561027318 37.0 35.0 37.0 35.0 37.0 5 35.8065376695985 37.0 35.0 37.0 35.0 37.0 6 35.84483254438494 37.0 35.0 37.0 35.0 37.0 7 35.77798294605094 37.0 35.0 37.0 33.0 37.0 8 35.76418993155672 37.0 35.0 37.0 33.0 37.0 9 37.35812061822911 39.0 37.0 39.0 34.0 39.0 10-11 37.33832379344835 39.0 37.0 39.0 34.0 39.0 12-13 37.25380867425011 39.0 37.0 39.0 33.5 39.0 14-15 38.47080490324487 40.0 38.0 41.0 34.0 41.0 16-17 38.45852824970715 40.0 38.0 41.0 34.0 41.0 18-19 38.46879562054494 40.0 38.0 41.0 34.0 41.0 20-21 38.430784509212344 40.0 38.0 41.0 34.0 41.0 22-23 38.32735089813478 40.0 38.0 41.0 34.0 41.0 24-25 38.211743746943974 40.0 37.5 41.0 33.5 41.0 26-27 38.083320483851864 40.0 37.0 41.0 33.0 41.0 28-29 37.99989256270058 40.0 37.0 41.0 33.0 41.0 30-31 38.026422833342906 40.0 37.0 41.0 33.0 41.0 32-33 37.85238994492409 40.0 37.0 41.0 33.0 41.0 34-35 37.73462290827054 40.0 37.0 41.0 33.0 41.0 36-37 37.58348640339203 40.0 36.5 41.0 32.5 41.0 38-39 37.458837370122524 40.0 36.0 41.0 32.0 41.0 40-41 37.38693961115027 40.0 36.0 41.0 32.0 41.0 42-43 37.243044790654096 40.0 36.0 41.0 31.5 41.0 44-45 37.078855679900755 40.0 36.0 41.0 31.0 41.0 46-47 36.84550970717253 39.0 35.5 41.0 30.5 41.0 48-49 36.616628171965345 39.0 35.0 41.0 30.0 41.0 50-51 36.42990009357159 39.0 35.0 41.0 30.0 41.0 52-53 36.22239784808806 39.0 35.0 40.5 29.0 41.0 54-55 36.02251470996913 39.0 35.0 40.0 29.0 41.0 56-57 35.79199647816722 38.0 35.0 40.0 28.0 41.0 58-59 35.53184856353546 38.0 34.0 40.0 28.0 41.0 60-61 35.27603559003451 38.0 34.0 40.0 27.0 41.0 62-63 34.98240769186572 37.0 34.0 40.0 26.5 41.0 64-65 34.634491052070594 36.5 34.0 39.5 26.0 41.0 66-67 34.280274966200494 36.0 33.0 39.0 26.0 41.0 68-69 33.910502824149916 35.5 33.0 39.0 26.0 40.0 70-71 33.53379533326376 35.0 33.0 38.0 25.0 40.0 72-73 33.17738008062634 35.0 32.0 37.5 25.0 40.0 74-75 32.805715986787405 35.0 32.0 37.0 24.0 39.0 76-77 31.77603954747937 34.0 30.5 36.0 23.5 38.5 78-79 32.23263136078647 35.0 31.5 36.0 24.0 38.5 80-81 32.11303891604706 35.0 32.0 35.5 24.0 37.5 82-83 31.84239864250847 34.5 31.0 35.0 23.5 37.0 84-85 31.570671537353 34.0 31.0 35.0 23.0 36.5 86-87 31.321187251047405 34.0 31.0 35.0 22.5 36.0 88-89 31.09056641882313 34.0 31.0 35.0 20.5 36.0 90-91 30.872231840604677 34.0 30.5 35.0 20.0 35.0 92-93 30.694061370296062 34.0 30.0 35.0 20.0 35.0 94-95 30.52994305383415 34.0 30.0 35.0 19.5 35.0 96-97 30.36217318834037 34.0 30.0 35.0 19.0 35.0 98-99 30.164619079809057 34.0 29.0 35.0 18.5 35.0 100 28.377704986849558 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23885834388847016 1101 2 -0.22206675959970568 1101 3 -0.24198879057596656 1101 4 -0.09341978483125501 1101 5 -0.1211065398141642 1101 6 -0.21206153833050934 1101 7 -0.15859026422680955 1101 8 -0.20462770812071085 1101 9 -0.40754776906787527 1101 10-11 -0.34411370502044036 1101 12-13 -0.35433653357108597 1101 14-15 -0.5497863361130513 1101 16-17 -0.5535768520846105 1101 18-19 -0.5769939051472264 1101 20-21 -0.49731494130617193 1101 22-23 -0.6833456013189192 1101 24-25 -0.6659733613437098 1101 26-27 -0.6826905574551603 1101 28-29 -0.6372245203221283 1101 30-31 -0.5636853410086786 1101 32-33 -0.5034804977905978 1101 34-35 -0.48350737406388333 1101 36-37 -0.49256799429639386 1101 38-39 -0.5334705584755142 1101 40-41 -0.5297686068800544 1101 42-43 -0.46868478354222276 1101 44-45 -0.4611949862135276 1101 46-47 -0.5477286260217795 1101 48-49 -0.45338668220306744 1101 50-51 -0.49610155054328686 1101 52-53 -0.5085860267791418 1101 54-55 -0.44020916833164137 1101 56-57 -0.5474258566340637 1101 58-59 -0.5242772250113887 1101 60-61 -0.47967172372258915 1101 62-63 -0.5330582007909541 1101 64-65 -0.5151757092243443 1101 66-67 -0.5708526460027699 1101 68-69 -0.5190240731669817 1101 70-71 -0.6198600735826005 1101 72-73 -0.4961765490021932 1101 74-75 -0.5859499951794973 1101 76-77 -0.4512113700549314 1101 78-79 -0.4599088600797252 1101 80-81 -0.39946475608565635 1101 82-83 -0.5053800565230198 1101 84-85 -0.5135253191577718 1101 86-87 -0.5244977543001497 1101 88-89 -0.4741539283356566 1101 90-91 -0.6501178221203858 1101 92-93 -0.7251230673753177 1101 94-95 -0.8026431712289934 1101 96-97 -0.8454298114339522 1101 98-99 -0.8898091257744589 1101 100 -0.6977453536040947 1102 1 -0.28876286929483896 1102 2 -0.2460678510783687 1102 3 -0.3043860399544158 1102 4 -0.1352924690882844 1102 5 -0.18549293755923912 1102 6 -0.2848187194796239 1102 7 -0.27618904691829016 1102 8 -0.29555425553895986 1102 9 -0.42473528116975956 1102 10-11 -0.3890702037570719 1102 12-13 -0.4654386946630922 1102 14-15 -0.5418045707178081 1102 16-17 -0.630330709943351 1102 18-19 -0.6482085683766101 1102 20-21 -0.6652590411550179 1102 22-23 -0.6659663952981489 1102 24-25 -0.7073770136267328 1102 26-27 -0.7782524634199888 1102 28-29 -0.81220374230994 1102 30-31 -0.6942074514946768 1102 32-33 -0.6423489644835243 1102 34-35 -0.6576176431979732 1102 36-37 -0.6926289133428796 1102 38-39 -0.7541304562268465 1102 40-41 -0.7920488440264322 1102 42-43 -0.7226634675359165 1102 44-45 -0.6824887399409079 1102 46-47 -0.7388488930289157 1102 48-49 -0.7255134033523092 1102 50-51 -0.7970973460864599 1102 52-53 -0.8692303268714312 1102 54-55 -0.8076122057642294 1102 56-57 -0.8884055183114228 1102 58-59 -0.8798505581381804 1102 60-61 -0.8917857069822119 1102 62-63 -0.9472995248165361 1102 64-65 -0.8696947704812032 1102 66-67 -0.9231361123054498 1102 68-69 -1.0599385559330017 1102 70-71 -0.9418466660303082 1102 72-73 -0.9910818995712063 1102 74-75 -0.9578807923342652 1102 76-77 -0.8144152531451709 1102 78-79 -0.7585982515863279 1102 80-81 -0.6760638289245762 1102 82-83 -0.60595621069319 1102 84-85 -0.6529536155037619 1102 86-87 -0.9231242308795302 1102 88-89 -0.8558278705337585 1102 90-91 -0.8211997145966308 1102 92-93 -0.9218418990165205 1102 94-95 -0.9226817840740047 1102 96-97 -1.0512555192119883 1102 98-99 -0.9821780693935089 1102 100 -1.0578560385963236 1103 1 -0.31531879131444285 1103 2 -0.2860749665138407 1103 3 -0.33273358133029873 1103 4 -0.16394860355245555 1103 5 -0.19698603993007424 1103 6 -0.2331609272056241 1103 7 -0.24950253476591655 1103 8 -0.31434916674996316 1103 9 -0.5008227937678171 1103 10-11 -0.4803966365930634 1103 12-13 -0.4654861952873617 1103 14-15 -0.7395958531503766 1103 16-17 -0.7024677393227918 1103 18-19 -0.6545001328861986 1103 20-21 -0.6094317886625333 1103 22-23 -0.7610504114053995 1103 24-25 -0.7151489410914351 1103 26-27 -0.7830689623330613 1103 28-29 -0.759759541056205 1103 30-31 -0.637823039800594 1103 32-33 -0.6317739441106411 1103 34-35 -0.6537251382521134 1103 36-37 -0.6663713116332985 1103 38-39 -0.6490891034652577 1103 40-41 -0.6743829784608693 1103 42-43 -0.7429831692729678 1103 44-45 -0.714289720110294 1103 46-47 -0.640290951105996 1103 48-49 -0.6428640146206632 1103 50-51 -0.7514897226664914 1103 52-53 -0.8042425365512997 1103 54-55 -0.7688784758255665 1103 56-57 -0.8810832809834395 1103 58-59 -0.9881373478838569 1103 60-61 -0.8708659696328596 1103 62-63 -0.8509041344199417 1103 64-65 -0.8856646091359508 1103 66-67 -0.8735466019885934 1103 68-69 -0.8757796307266688 1103 70-71 -0.8668363610581551 1103 72-73 -0.9093015703201743 1103 74-75 -0.8195097470758625 1103 76-77 -0.8557677220596283 1103 78-79 -0.7169006641497653 1103 80-81 -0.6250752305962948 1103 82-83 -0.6839456213611683 1103 84-85 -0.6207742107676495 1103 86-87 -0.7081421710721756 1103 88-89 -0.6837779168519873 1103 90-91 -0.8826541667367813 1103 92-93 -0.8948195167691928 1103 94-95 -0.7989381507088176 1103 96-97 -0.9437075792874303 1103 98-99 -1.1092379370379781 1103 100 -0.9973797539634504 1104 1 -0.35118346184143334 1104 2 -0.3196848945551878 1104 3 -0.37126865189877023 1104 4 -0.20267784134858147 1104 5 -0.25366610776735854 1104 6 -0.3257583002543427 1104 7 -0.31383860242082307 1104 8 -0.3075875654397038 1104 9 -0.46567969894671535 1104 10-11 -0.42447993930665007 1104 12-13 -0.535304930821205 1104 14-15 -0.6797798868043827 1104 16-17 -0.7017987424258934 1104 18-19 -0.7101128397259799 1104 20-21 -0.7397684539613749 1104 22-23 -0.7685279449999669 1104 24-25 -0.793685170516973 1104 26-27 -0.8568345169439482 1104 28-29 -0.8290552822732593 1104 30-31 -0.817103787396384 1104 32-33 -0.7839035758511201 1104 34-35 -0.8217348833795484 1104 36-37 -0.7876998468290282 1104 38-39 -0.8005736530062322 1104 40-41 -0.8733888813680863 1104 42-43 -0.6768784197693591 1104 44-45 -0.7686586391838546 1104 46-47 -0.7735012634364153 1104 48-49 -0.8135976326059406 1104 50-51 -0.77875373489114 1104 52-53 -0.6350270548467094 1104 54-55 -0.7401549406790053 1104 56-57 -0.7919621718153067 1104 58-59 -0.9812842225681564 1104 60-61 -0.9791090585088185 1104 62-63 -0.9579870252196159 1104 64-65 -0.8776434911171478 1104 66-67 -0.8660754940720707 1104 68-69 -0.846655664087443 1104 70-71 -0.9511470120832684 1104 72-73 -0.8899207196006316 1104 74-75 -0.8433649371015974 1104 76-77 -0.8365676005193237 1104 78-79 -0.856938566710113 1104 80-81 -0.9064787204882023 1104 82-83 -1.0086571754678353 1104 84-85 -0.8380554125191928 1104 86-87 -0.7378210648542662 1104 88-89 -0.7335550184621873 1104 90-91 -0.7299418936246767 1104 92-93 -0.9466526397872883 1104 94-95 -0.9897843644534667 1104 96-97 -1.0637580471214925 1104 98-99 -1.1092641705670125 1104 100 -1.0680654531532099 1105 1 -0.3990624891986414 1105 2 -0.38393096293994944 1105 3 -0.4382334771028411 1105 4 -0.2422689937906668 1105 5 -0.33440538792683583 1105 6 -0.3295126678704676 1105 7 -0.3846081657331055 1105 8 -0.33629907195103215 1105 9 -0.5309347529490935 1105 10-11 -0.5547054295794354 1105 12-13 -0.5585647960478113 1105 14-15 -0.7418382348522101 1105 16-17 -0.658783591938203 1105 18-19 -0.6938073898017549 1105 20-21 -0.727854066627394 1105 22-23 -0.7391899860579443 1105 24-25 -0.7449697593416076 1105 26-27 -0.7722632757889443 1105 28-29 -0.8161088160539762 1105 30-31 -0.7159837747814777 1105 32-33 -0.669554326746379 1105 34-35 -0.6647336457977531 1105 36-37 -0.7959598468116411 1105 38-39 -0.7655159806945946 1105 40-41 -0.6566099596856745 1105 42-43 -0.7669583018274295 1105 44-45 -0.8151568793138466 1105 46-47 -0.8686890231134186 1105 48-49 -0.757425568309074 1105 50-51 -0.8088942799748509 1105 52-53 -0.8586979041660214 1105 54-55 -0.8536487064747931 1105 56-57 -0.8692735144894002 1105 58-59 -0.8474444457241148 1105 60-61 -0.9660354163060134 1105 62-63 -0.8039192575094134 1105 64-65 -0.8724464463164239 1105 66-67 -0.9405904593789174 1105 68-69 -0.9738128469739564 1105 70-71 -0.8678487261797017 1105 72-73 -0.9027261267072291 1105 74-75 -1.0174362834532324 1105 76-77 -0.9413302636719045 1105 78-79 -0.8386641032781128 1105 80-81 -0.7635921152789678 1105 82-83 -0.7988358478081636 1105 84-85 -0.6807354063378028 1105 86-87 -0.7709851978906137 1105 88-89 -0.8319861324944604 1105 90-91 -0.7690397738691885 1105 92-93 -0.8054785226347612 1105 94-95 -0.9257867796289041 1105 96-97 -1.017260350362502 1105 98-99 -1.0078987716066337 1105 100 -1.0812518720079254 1106 1 -0.34039188194970293 1106 2 -0.3289384031079834 1106 3 -0.35638154614151674 1106 4 -0.21069997225225734 1106 5 -0.24259636687239805 1106 6 -0.27791413694883005 1106 7 -0.2522912960110446 1106 8 -0.29734184644111394 1106 9 -0.4280063530684117 1106 10-11 -0.44194942319298036 1106 12-13 -0.5116329482351887 1106 14-15 -0.6332240357572516 1106 16-17 -0.6505155665741569 1106 18-19 -0.6240329541663812 1106 20-21 -0.6424662913981791 1106 22-23 -0.6617812539547216 1106 24-25 -0.6840334413786238 1106 26-27 -0.6592369011572146 1106 28-29 -0.7515390079192557 1106 30-31 -0.7496054543747874 1106 32-33 -0.6900366990625315 1106 34-35 -0.7374288495767587 1106 36-37 -0.6775046236244435 1106 38-39 -0.6657855423335093 1106 40-41 -0.6958575247039605 1106 42-43 -0.6141350942735002 1106 44-45 -0.7465118476909964 1106 46-47 -0.8048954960363375 1106 48-49 -0.7966607239988335 1106 50-51 -0.7894791874702918 1106 52-53 -0.8276300643834844 1106 54-55 -0.9091078182802121 1106 56-57 -0.9617882385454877 1106 58-59 -1.0064956625906234 1106 60-61 -1.0965260982187885 1106 62-63 -0.9022452313353497 1106 64-65 -0.8870485419727956 1106 66-67 -0.8805785200537102 1106 68-69 -0.938225607300339 1106 70-71 -0.9214063022841756 1106 72-73 -0.8917721797419063 1106 74-75 -0.8169024263841891 1106 76-77 -0.7899544879531426 1106 78-79 -0.671919955866457 1106 80-81 -0.6839752207227363 1106 82-83 -0.7126500751963931 1106 84-85 -0.7873077279820144 1106 86-87 -0.7366735984103769 1106 88-89 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66-67 1.0560147855664113 2310 68-69 0.8885802936435567 2310 70-71 0.8984479258170879 2310 72-73 1.0625313506033294 2310 74-75 1.0642485196308513 2310 76-77 1.0126835394765514 2310 78-79 0.8576262605739515 2310 80-81 0.8613747301118124 2310 82-83 0.8404412346305321 2310 84-85 0.8486357311886863 2310 86-87 0.9905156206513013 2310 88-89 1.0201351101729088 2310 90-91 1.0053847267316272 2310 92-93 1.078303664106027 2310 94-95 1.0955400189078723 2310 96-97 1.1538483877709176 2310 98-99 1.1280862350636731 2310 100 1.2863663119428956 2311 1 0.3505872410120787 2311 2 0.42866624005698384 2311 3 0.4767457314883927 2311 4 0.29445512457333933 2311 5 -0.05993411542470284 2311 6 0.11925748172823347 2311 7 0.3490139958721912 2311 8 0.15926442170929533 2311 9 0.3590689888814538 2311 10-11 0.507154524074636 2311 12-13 0.545824519004185 2311 14-15 0.6975347037357125 2311 16-17 0.8319570571808299 2311 18-19 0.8821597414602707 2311 20-21 0.944949452574626 2311 22-23 0.8474248703653444 2311 24-25 0.7507175457866495 2311 26-27 0.813039008047042 2311 28-29 0.7764239475633588 2311 30-31 0.8582058648068198 2311 32-33 1.0337483792824358 2311 34-35 0.9502615843761362 2311 36-37 1.0087231646790684 2311 38-39 1.0855719293568313 2311 40-41 1.0582411434335768 2311 42-43 1.1542448304622255 2311 44-45 1.0690852314157553 2311 46-47 1.0991366398337732 2311 48-49 1.038226596840154 2311 50-51 1.1225413478958899 2311 52-53 1.1062374909244994 2311 54-55 1.130599048152007 2311 56-57 1.1767545439867533 2311 58-59 1.162874540749229 2311 60-61 1.192384136108501 2311 62-63 1.2206546923867023 2311 64-65 1.2647430431344375 2311 66-67 1.251319972949723 2311 68-69 1.300001803288275 2311 70-71 1.2985577348365425 2311 72-73 1.305971326563565 2311 74-75 1.1692784650641173 2311 76-77 1.1853890024064562 2311 78-79 1.0382688857041913 2311 80-81 1.066553118565892 2311 82-83 1.081746403259018 2311 84-85 1.0993658979521825 2311 86-87 1.1218787598764237 2311 88-89 1.1624809241874914 2311 90-91 1.1636335105723603 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0.9575401719802841 2312 52-53 0.9600874798530157 2312 54-55 0.9451697197625677 2312 56-57 1.0488329438613206 2312 58-59 1.0013854098310802 2312 60-61 1.0060846072540954 2312 62-63 0.9993894392943687 2312 64-65 1.0643817184254019 2312 66-67 1.062693987919836 2312 68-69 1.0667536722605533 2312 70-71 0.9542058660476371 2312 72-73 1.0141615672434625 2312 74-75 0.9949136084822641 2312 76-77 0.952277603085232 2312 78-79 0.8903424168755834 2312 80-81 0.7915598462701467 2312 82-83 0.86408188166412 2312 84-85 0.6922364340000655 2312 86-87 0.8752116210097256 2312 88-89 0.5522651746203806 2312 90-91 0.9142561269364293 2312 92-93 1.092415156892006 2312 94-95 1.1571104254493427 2312 96-97 1.2036138170812372 2312 98-99 1.2359166975215103 2312 100 1.339862008747403 2313 1 -0.39317008491749306 2313 2 -0.0922956211688657 2313 3 0.19716551273679528 2313 4 0.0459427194113502 2313 5 0.20990537593051783 2313 6 0.34315134370338995 2313 7 0.04733047818945835 2313 8 -0.032193602348257855 2313 9 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2315 58-59 0.8621418471651907 2315 60-61 0.912242486384585 2315 62-63 0.9290990483030512 2315 64-65 0.9584142946769205 2315 66-67 0.9749439249163814 2315 68-69 0.8808893776448699 2315 70-71 0.4575694699374324 2315 72-73 0.710930660756766 2315 74-75 0.894946150630993 2315 76-77 0.8651909403952978 2315 78-79 0.7579945095149299 2315 80-81 0.8089604597734592 2315 82-83 0.8165773818305837 2315 84-85 0.7584845323363112 2315 86-87 0.7266299644929788 2315 88-89 0.8669264674974073 2315 90-91 0.9237878696927666 2315 92-93 0.9593913785809143 2315 94-95 1.046178127569032 2315 96-97 1.053492867798436 2315 98-99 0.8921515246724105 2315 100 0.8407027579913624 2316 1 0.49841984544912776 2316 2 0.4970820941734715 2316 3 0.4708811493493883 2316 4 0.31244673101679865 2316 5 0.4044931981358104 2316 6 0.4198346614600439 2316 7 0.5025263286710526 2316 8 0.4696827969548991 2316 9 0.5475130317014987 2316 10-11 0.5181755375355834 2316 12-13 0.6856446114132524 2316 14-15 0.791772897842435 2316 16-17 0.8132306960533668 2316 18-19 0.8258614154848587 2316 20-21 0.8796582598833851 2316 22-23 0.871486833503397 2316 24-25 0.9486533545272593 2316 26-27 0.9625341496345499 2316 28-29 0.9286761394823984 2316 30-31 0.7854409504832489 2316 32-33 0.7555443138609448 2316 34-35 0.6865776348674473 2316 36-37 0.7057200073946603 2316 38-39 0.6686559153541651 2316 40-41 0.7462950838621936 2316 42-43 0.8993648397608709 2316 44-45 0.8100973246191074 2316 46-47 0.8451405958477807 2316 48-49 0.5317146685486378 2316 50-51 0.6885333866631385 2316 52-53 0.8210502537563826 2316 54-55 0.8758722963085503 2316 56-57 0.9335847544556302 2316 58-59 0.8436594456946764 2316 60-61 0.9152510772113658 2316 62-63 0.8704674733343509 2316 64-65 0.8639721723496976 2316 66-67 0.8338514867714011 2316 68-69 0.7920450191298087 2316 70-71 0.6605337759871261 2316 72-73 0.7588133135022233 2316 74-75 0.8568143824758963 2316 76-77 0.7378123604789018 2316 78-79 0.6960476547840244 2316 80-81 0.7050270056359977 2316 82-83 0.6905198852257115 2316 84-85 0.7257189916619566 2316 86-87 0.7461082484849442 2316 88-89 0.6454278162656131 2316 90-91 0.7477626223964897 2316 92-93 0.7495957008282055 2316 94-95 0.694721725362907 2316 96-97 0.9436501533571082 2316 98-99 0.9929856522656202 2316 100 0.7035548921484853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 38.0 13 190.0 14 866.0 15 2673.0 16 6307.0 17 12876.0 18 20660.0 19 28751.0 20 38439.0 21 50338.0 22 59726.0 23 61060.0 24 61329.0 25 65670.0 26 75636.0 27 90572.0 28 110599.0 29 133514.0 30 161568.0 31 200475.0 32 251278.0 33 325877.0 34 452971.0 35 625391.0 36 942745.0 37 1472357.0 38 1357778.0 39 212873.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.220726854167665 19.68414400994155 13.10302876189479 23.992100373995996 2 16.052524967079922 21.06726718205302 39.619739383201434 23.260468467665625 3 18.3601843524389 26.701554718857256 30.843196653936396 24.09506427476745 4 12.668097604796035 17.285760944008707 38.19924616279506 31.846895288400194 5 12.65960308194506 37.78576626848804 34.69800207114447 14.856628578422427 6 29.644520264539103 37.37386558727098 18.73173254176607 14.249881606423841 7 26.31908812115312 33.33182363897023 22.77863498126 17.570453258616652 8 25.717285845384406 35.22540442669679 20.78132266793149 18.275987059987315 9 27.067269674523796 15.568156084347812 20.683301416716684 36.68127282441171 10-11 24.696379062396 25.98147446360979 29.01342955181499 20.30871692217922 12-13 25.1496468409306 24.24026718404799 28.46711712299322 22.14296885202819 14-15 22.774556205472326 25.71739325120706 26.478534596061344 25.029515947259274 16-17 21.87215581323102 28.115579762109004 27.345343113311195 22.66692131134878 18-19 21.444719478719502 27.510515077571785 29.41924936358424 21.62551608012448 20-21 22.373776911178656 26.970181234878343 29.102189009646345 21.553852844296653 22-23 22.347808396349535 26.741612855188006 28.889992413431624 22.020586335030835 24-25 21.9443247235155 26.58270233421245 29.295913664325933 22.17705927794612 26-27 21.9006187318924 26.784919524986396 29.097042198164374 22.217419544956833 28-29 21.67444593550456 27.552470214972825 28.996329578534365 21.776754270988253 30-31 22.601587668835197 26.79026081057897 29.1525043134731 21.45564720711274 32-33 22.40764784619753 26.913225909397354 28.74048633223159 21.93863991217352 34-35 22.422408737721742 27.043410057176366 29.05917020964668 21.475010995455214 36-37 22.107104369946263 26.83151404965948 28.998321225139108 22.06306035525515 38-39 22.01200640767866 26.894614151692863 29.09414939414539 21.999230046483085 40-41 22.007303116261077 26.295813584534972 29.591552563719787 22.105330735484166 42-43 21.7331116343174 26.663134706630142 28.967984142970067 22.635769516082387 44-45 21.265498422040814 27.02489737346881 29.334466148178134 22.375138056312245 46-47 21.27779078788865 26.489736488450138 28.99054366504718 23.24192905861403 48-49 21.495357035303915 26.59862022585518 29.02400228899651 22.882020449844394 50-51 21.351337346348405 26.812803174019383 28.83368112324941 23.0021783563828 52-53 21.07704234888015 27.086976066678446 28.602409333000757 23.23357225144064 54-55 20.852415756747238 26.76263251177341 28.938003128435145 23.446948603044206 56-57 21.319558748743013 26.607338811731374 28.388962694277204 23.68413974524841 58-59 20.75125206601061 26.741358082193926 28.63338441543269 23.87400543636277 60-61 21.286219915140737 26.86320098761849 28.200612021521522 23.64996707571925 62-63 21.18567514222755 26.712498230125593 28.056049100933116 24.045777526713742 64-65 20.753651917770846 26.5300393078776 27.998554230079137 24.717754544272417 66-67 20.986008359952322 26.521201257592804 27.67980639153409 24.812983990920788 68-69 20.78060692949894 26.642858148473174 27.769492735429797 24.80704218659809 70-71 20.945841463134762 26.497530136325505 27.65921035777334 24.897418042766393 72-73 21.02992920056048 26.922621802192076 27.11204006904573 24.935408928201706 74-75 21.26310154892567 27.05184965156613 26.764392259227588 24.920656540280614 76-77 20.999864309428457 27.027538588426598 26.8481426042664 25.12445449787854 78-79 20.8965290431823 27.205659180993425 26.791396859272993 25.106414916551284 80-81 20.82792013508939 27.389834041180688 26.599912011023026 25.182333812706897 82-83 21.184398488345064 26.995149277501046 26.741518309642952 25.078933924510938 84-85 21.294343388277355 27.274658270968043 26.26567136382933 25.16532697692527 86-87 20.78095182371203 27.428968535643477 26.47566960310336 25.314410037541137 88-89 20.869845400513483 26.981433806329292 26.526958481618156 25.62176231153907 90-91 21.273933913349474 26.62677299277699 26.466953925555835 25.632339168317703 92-93 21.201150979441632 26.691247851516525 26.435771935533364 25.671829233508475 94-95 20.725133524130428 26.482694467956737 26.73893590980993 26.053236098102907 96-97 20.78791909664393 26.415513744372802 26.776790213031827 26.019776945951445 98-99 20.663246326026815 26.674229523568606 26.56498689975474 26.097537250649843 100 20.618685769189383 26.75964310604815 26.64989859701189 25.971772527750588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2709.0 1 1958.0 2 2470.5 3 3572.5 4 2538.0 5 1845.0 6 2552.0 7 4174.5 8 6085.0 9 6977.0 10 6096.0 11 5043.5 12 5244.0 13 5751.0 14 6385.0 15 7235.0 16 8111.0 17 8997.0 18 9908.5 19 10871.5 20 12158.0 21 13638.0 22 15473.0 23 18092.0 24 21397.0 25 25625.0 26 31476.5 27 38762.5 28 46654.0 29 56529.0 30 69507.5 31 82123.0 32 96875.5 33 112016.5 34 125499.5 35 139329.0 36 154344.0 37 173148.0 38 184392.5 39 187675.5 40 190597.0 41 194545.0 42 202451.0 43 214653.0 44 228949.5 45 246088.0 46 261796.0 47 274999.5 48 289996.0 49 300159.0 50 303412.0 51 303808.0 52 295948.5 53 278039.5 54 257380.0 55 230925.0 56 202316.0 57 172469.0 58 144788.5 59 122444.0 60 96665.0 61 73355.0 62 56935.5 63 43548.5 64 31930.0 65 22836.5 66 16529.5 67 12629.0 68 9848.0 69 7364.0 70 5663.5 71 4395.5 72 3439.5 73 2802.0 74 2197.0 75 1610.5 76 1112.0 77 738.5 78 473.0 79 306.0 80 201.0 81 122.0 82 77.0 83 45.5 84 34.5 85 27.0 86 19.0 87 9.5 88 4.5 89 4.0 90 3.0 91 3.5 92 2.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051695955667236934 2 0.0 3 0.0 4 0.013953657441227547 5 0.003942787659338456 6 1.465720319456675E-5 7 1.6122923514023425E-4 8 3.6643007986416877E-4 9 0.0017442071801534434 10-11 8.134747772984547E-4 12-13 3.0780126708590175E-4 14-15 1.465720319456675E-5 16-17 8.061461757011713E-5 18-19 0.0 20-21 1.465720319456675E-5 22-23 0.0 24-25 6.595741437555037E-5 26-27 7.328601597283375E-6 28-29 0.0010333328252169558 30-31 9.600468092441222E-4 32-33 0.01393900023803298 34-35 0.023217009860193732 36-37 0.03127847161720545 38-39 0.020417484050031482 40-41 2.491724543076348E-4 42-43 0.001773521586542577 44-45 0.009373281442925437 46-47 0.014166186887548766 48-49 0.005577065815532648 50-51 0.01022339922821031 52-53 0.01567587881658914 54-55 0.00942458165410642 56-57 0.0031146556788454348 58-59 0.008200705187360097 60-61 0.0016342781561941929 62-63 9.673754108414055E-4 64-65 0.01912765016890961 66-67 0.04466782673544217 68-69 0.05475931113490139 70-71 0.048082955079776224 72-73 0.040395252004225966 74-75 0.02815648733676273 76-77 0.028119844328776313 78-79 0.012011578017947451 80-81 0.0020300226424474948 82-83 0.027079182901962075 84-85 0.04718153708331037 86-87 0.04784843982866316 88-89 0.059537559376330144 90-91 0.03475955737591505 92-93 0.026155779100704366 94-95 0.007365244605269792 96-97 0.003613000587460704 98-99 0.02240353508289528 100 0.01884916330821284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6822584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.43972757526277 #Duplication Level Percentage of deduplicated Percentage of total 1 76.54283177646626 47.7931356396172 2 14.61211658124611 18.247531572619742 3 4.373608389990015 8.192607491755805 4 1.7204633946410524 4.2970106265839645 5 0.8135436444519429 2.5398721765082874 6 0.48901673838167214 1.8320443154577093 7 0.3004415517005896 1.3131642048331829 8 0.20490147333397454 1.0235193739794672 9 0.1469672669141019 0.8258936497737707 >10 0.7409912517855449 7.872461283748944 >50 0.034947814597299065 1.501154468084337 >100 0.01809482718205096 2.067032043932835 >500 0.0013558653776881433 0.5584765375337659 >1k 5.810731757652245E-4 0.704994801148473 >5k 5.5340302427336465E-5 0.17942337968420602 >10k+ 8.30104536410047E-5 1.0516784347383057 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25667 0.3762064343949448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21974 0.3220773829974098 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13230 0.19391479826411812 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.465720319456675E-5 2 0.0 0.0 0.0 0.0 1.465720319456675E-5 3 0.0 0.0 0.0 0.0 1.465720319456675E-5 4 0.0 0.0 0.0 0.0 1.465720319456675E-5 5 0.0 0.0 0.0 0.0 1.465720319456675E-5 6 0.0 0.0 0.0 0.0 2.93144063891335E-5 7 0.0 0.0 0.0 0.0 2.93144063891335E-5 8 0.0 0.0 0.0 0.0 4.397160958370025E-5 9 0.0 0.0 0.0 8.79432191674005E-5 7.328601597283375E-5 10-11 0.0 0.0 0.0 1.0992902395925064E-4 8.79432191674005E-5 12-13 0.0 0.0 0.0 1.465720319456675E-4 1.6122923514023425E-4 14-15 0.0 0.0 0.0 1.6122923514023425E-4 1.9054364152936776E-4 16-17 0.0 0.0 0.0 2.4184385271035138E-4 2.1985804791850125E-4 18-19 0.0 0.0 0.0 3.224584702804685E-4 2.34515251113068E-4 20-21 0.0 0.0 0.0 7.108743549364873E-4 2.5650105590491814E-4 22-23 0.0 0.0 0.0 0.0025063817462709142 2.7848686069676824E-4 24-25 0.0 0.0 0.0 0.006984157322211056 2.93144063891335E-4 26-27 0.0 0.0 0.0 0.015206848314363003 2.93144063891335E-4 28-29 0.0 0.0 0.0 0.042344660029103344 2.93144063891335E-4 30-31 0.0 0.0 0.0 0.12849970040676673 3.004726654886184E-4 32-33 0.0 0.0 0.0 0.32246579888206583 3.3711567347503526E-4 34-35 0.0 0.0 0.0 0.6152874043031203 3.737586814614521E-4 36-37 0.0 0.0 0.0 1.0538162666813629 4.1040168944786904E-4 38-39 0.0 0.0 0.0 1.6592100588281506 4.1773029104515244E-4 40-41 0.0 0.0 0.0 2.309638107790245 4.397160958370025E-4 42-43 0.0 0.0 0.0 2.916871378937951 4.397160958370025E-4 44-45 0.0 0.0 0.0 3.6358804816474226 4.4704469743428584E-4 46-47 0.0 0.0 0.0 4.4446576253220185 4.6170190062885265E-4 48-49 0.0 0.0 0.0 5.258462189692351 4.910163070179862E-4 50-51 0.0 0.0 0.0 6.071079520603924 5.056735102125529E-4 52-53 0.0 0.0 0.0 6.8502124708175085 5.569737213935365E-4 54-55 0.0 0.0 0.0 7.686603785310668 5.8628812778267E-4 56-57 0.0 0.0 0.0 8.591627453762387 6.009453309772368E-4 58-59 0.0 0.0 0.0 9.565921064511627 6.009453309772368E-4 60-61 0.0 0.0 0.0 10.513956295737803 6.009453309772368E-4 62-63 0.0 0.0 0.0 11.463984906598437 6.888885501446373E-4 64-65 0.0 0.0 0.0 12.369778078217873 6.888885501446373E-4 66-67 0.0 4.397160958370025E-5 0.0 13.357461044085351 6.962171517419207E-4 68-69 0.0 4.397160958370025E-5 0.0 14.44095814723571 7.03545753339204E-4 70-71 0.0 4.397160958370025E-5 0.0 15.532560683752667 7.03545753339204E-4 72-73 0.0 4.397160958370025E-5 0.0 16.536322601524585 7.548459645201877E-4 74-75 0.0 4.397160958370025E-5 0.0 17.49743938660191 7.841603709093212E-4 76-77 0.0 4.397160958370025E-5 0.0 18.523113823149707 8.061461757011713E-4 78-79 0.0 4.397160958370025E-5 0.0 19.61113267348559 8.061461757011713E-4 80-81 0.0 4.397160958370025E-5 0.0 20.66883309901351 8.134747772984547E-4 82-83 0.0 4.397160958370025E-5 0.0 21.70998554213477 8.647749884794383E-4 84-85 0.0 4.397160958370025E-5 0.0 22.71562358191559 8.867607932712884E-4 86-87 7.328601597283375E-6 5.130021118098363E-5 0.0 23.71204224088703 9.014179964658552E-4 88 1.465720319456675E-5 5.8628812778267E-5 0.0 24.47074304984739 9.087465980631385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11910 0.0 54.79462 1 GTATCAA 21220 0.0 40.59199 1 TACCTGG 5205 0.0 38.46291 2 ATCAACG 25905 0.0 32.70886 3 CAACGCA 26335 0.0 32.18815 5 TATCAAC 27080 0.0 31.601997 2 ACCTGGG 6285 0.0 31.03102 3 TCAACGC 27455 0.0 30.948738 4 AACGCAG 28010 0.0 30.681786 6 GTGGTAT 4755 0.0 28.180882 1 GTACATG 23220 0.0 27.84202 1 ACGCAGA 31435 0.0 27.084743 7 TACATGG 23095 0.0 27.084034 2 CGCAGAG 31800 0.0 26.744316 8 ACATGGG 23240 0.0 25.883745 3 TGGTATC 5325 0.0 24.358063 2 CATGGGG 17385 0.0 24.167337 4 ATGGGGG 13120 0.0 24.138977 5 GCAGAGT 34815 0.0 23.955873 9 TGGGGGG 13530 0.0 23.615175 6 >>END_MODULE