FastQCFastQC Report
Mon 6 Jun 2016
ERR1211381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7144138
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263140.36832995107317357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208710.29214161316592707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.17474466478671044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119650.050.482721
TACCTGG64300.045.087782
GTATCAA213100.040.4166371
ACCTGGG82250.034.404173
ATCAACG256550.033.1632463
CAACGCA260450.032.7927635
TATCAAC264100.032.397732
TCAACGC270400.031.603244
AACGCAG277550.031.1959976
GTACATG224600.030.5854741
TACATGG229150.029.2673992
ACATGGG228250.028.2343083
ACGCAGA304700.028.000377
CGCAGAG310750.027.346668
CATGGGG180650.026.9306454
CCTGGGG81550.026.5141834
ATGGGGG141450.026.352255
GTACCTG112850.025.9273131
ATAACGC6550.025.1175483
GTACTAG22950.025.046361
Produced by FastQC (version 0.11.5)