##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211381_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7144138 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360174873441693 31.0 31.0 34.0 28.0 34.0 2 31.39925558548841 31.0 31.0 34.0 28.0 34.0 3 31.46490493324737 31.0 31.0 34.0 28.0 34.0 4 35.10077282941623 37.0 35.0 37.0 32.0 37.0 5 35.153380435820246 37.0 35.0 37.0 32.0 37.0 6 35.21669584210159 37.0 35.0 37.0 32.0 37.0 7 35.14519134428814 37.0 35.0 37.0 32.0 37.0 8 35.147124397653016 37.0 35.0 37.0 32.0 37.0 9 36.71814920148519 39.0 37.0 39.0 32.0 39.0 10-11 36.64621127139481 39.0 37.0 39.0 32.0 39.0 12-13 36.554201850524166 39.0 37.0 39.0 32.0 39.0 14-15 37.74210947492895 40.0 37.0 41.0 32.0 41.0 16-17 37.72578168282864 40.0 37.0 41.0 32.0 41.0 18-19 37.724042634674745 40.0 37.0 41.0 32.0 41.0 20-21 37.70176639924929 40.0 37.0 41.0 32.0 41.0 22-23 37.57881853626008 40.0 37.0 41.0 32.0 41.0 24-25 37.487430743919006 40.0 37.0 41.0 31.5 41.0 26-27 37.34623722274122 40.0 36.5 41.0 31.0 41.0 28-29 37.20525737324783 40.0 36.0 41.0 31.0 41.0 30-31 37.03645443299108 39.0 36.0 41.0 30.5 41.0 32-33 36.8209654544747 39.0 35.5 41.0 30.0 41.0 34-35 36.69386369636197 39.0 35.0 41.0 30.0 41.0 36-37 36.558193794688734 39.0 35.0 41.0 30.0 41.0 38-39 36.394177646064506 39.0 35.0 40.5 29.5 41.0 40-41 36.2213943095724 39.0 35.0 40.0 29.0 41.0 42-43 36.017667141928115 38.5 35.0 40.0 28.0 41.0 44-45 35.87597607996934 38.0 35.0 40.0 27.5 41.0 46-47 35.64456124727714 38.0 34.5 40.0 26.5 41.0 48-49 35.39208663102532 38.0 34.0 40.0 26.0 41.0 50-51 34.72055166067621 37.5 33.5 39.5 25.0 40.5 52-53 35.01720830420689 38.0 34.0 39.5 26.0 40.5 54-55 35.42049964600348 38.0 34.0 40.0 26.0 41.0 56-57 35.34034532927556 38.0 34.0 40.0 26.0 41.0 58-59 35.13578678071448 38.0 34.0 40.0 26.0 41.0 60-61 34.90577015729539 38.0 34.0 40.0 26.0 41.0 62-63 34.61073106370566 37.0 33.5 40.0 25.5 41.0 64-65 34.392092439983664 37.0 33.0 40.0 25.0 41.0 66-67 34.18796060490433 36.0 33.0 39.5 25.0 41.0 68-69 33.84687417852231 36.0 33.0 39.0 24.0 41.0 70-71 33.47298477437026 35.0 32.5 39.0 24.0 41.0 72-73 33.08343063921778 35.0 32.0 38.0 23.0 40.0 74-75 32.70703253492584 35.0 32.0 37.0 22.5 39.5 76-77 32.31345328155755 35.0 31.0 37.0 21.5 39.0 78-79 31.90966635862857 35.0 31.0 36.0 20.0 39.0 80-81 31.505203566896384 35.0 31.0 36.0 19.5 37.5 82-83 31.12365823560519 34.5 30.0 35.0 18.5 37.0 84-85 30.78515406617285 34.0 30.0 35.0 18.0 37.0 86-87 30.43994817849263 34.0 29.5 35.0 17.0 36.0 88-89 30.110082909932594 34.0 29.0 35.0 15.0 36.0 90-91 29.798378404784454 34.0 29.0 35.0 11.5 36.0 92-93 29.497683625372296 34.0 28.5 35.0 10.0 35.0 94-95 29.202750282819284 34.0 27.0 35.0 10.0 35.0 96-97 28.89238834692163 34.0 27.0 35.0 10.0 35.0 98-99 28.562107912809076 34.0 25.5 35.0 9.0 35.0 100 27.273412411686337 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.32851515931333 1101 2 -0.2976398202041324 1101 3 -0.383389838378406 1101 4 -0.25082186951293295 1101 5 -0.14511104356429172 1101 6 -0.32149346910883736 1101 7 -0.25415033882927673 1101 8 -0.3254826339870718 1101 9 -0.4771290116313125 1101 10-11 -0.43535960578616084 1101 12-13 -0.45585169274399107 1101 14-15 -0.6958480863207228 1101 16-17 -0.6504774182633497 1101 18-19 -0.6501221838752187 1101 20-21 -0.7083759040547051 1101 22-23 -0.6583638089135491 1101 24-25 -0.7305403870873803 1101 26-27 -0.812376742720545 1101 28-29 -0.7694950054149672 1101 30-31 -0.8419684600261448 1101 32-33 -0.7806282292315387 1101 34-35 -0.833942453673167 1101 36-37 -0.8554242102538723 1101 38-39 -0.8865333891150655 1101 40-41 -1.0477285127101865 1101 42-43 -0.9170259758655277 1101 44-45 -0.983268191933405 1101 46-47 -1.042871101909995 1101 48-49 -1.1310599552735425 1101 50-51 -0.9635148465893835 1101 52-53 -0.8455614311872708 1101 54-55 -0.789714674154034 1101 56-57 -0.8402811366726581 1101 58-59 -0.8192200804791554 1101 60-61 -0.7906751675751735 1101 62-63 -0.8437990439479677 1101 64-65 -0.8282586685773978 1101 66-67 -0.8220844508951259 1101 68-69 -0.9112845102749105 1101 70-71 -0.9931524173606903 1101 72-73 -1.0459262608183977 1101 74-75 -1.0657329244936093 1101 76-77 -1.1083740121730798 1101 78-79 -1.1929408354246824 1101 80-81 -1.3119541271945963 1101 82-83 -1.3008766858478005 1101 84-85 -1.3329558582562662 1101 86-87 -1.416890444432962 1101 88-89 -1.6491549360010147 1101 90-91 -1.5360651710121296 1101 92-93 -1.7478069349082688 1101 94-95 -1.7776035564370218 1101 96-97 -1.971669503210773 1101 98-99 -2.023446747167565 1101 100 -1.867411507547466 1102 1 -0.384092054338236 1102 2 -0.34705990778437723 1102 3 -0.45082096761930046 1102 4 -0.3680655308702967 1102 5 -0.32410239333185586 1102 6 -0.3233772843077247 1102 7 -0.4247254093355366 1102 8 -0.41864418197109643 1102 9 -0.5819268861706703 1102 10-11 -0.5343329946363298 1102 12-13 -0.5722276510174211 1102 14-15 -0.7487100085172713 1102 16-17 -0.8004132517735627 1102 18-19 -0.7535658050517426 1102 20-21 -0.8324610014146927 1102 22-23 -0.7804898567724337 1102 24-25 -0.8457942610073346 1102 26-27 -0.8861761209640591 1102 28-29 -0.9680003346450832 1102 30-31 -0.8260549861553415 1102 32-33 -0.8452068252964722 1102 34-35 -1.0106349362947356 1102 36-37 -0.9914948995085666 1102 38-39 -1.0742173003427595 1102 40-41 -1.0967852292677804 1102 42-43 -1.1329147995159587 1102 44-45 -1.113136104543024 1102 46-47 -1.1759347181344424 1102 48-49 -1.1625238912204239 1102 50-51 -1.0793471972148083 1102 52-53 -0.9493439360160707 1102 54-55 -0.990901675783654 1102 56-57 -0.9117783668217783 1102 58-59 -0.8913374697475689 1102 60-61 -0.9477464531721793 1102 62-63 -0.9967830581375665 1102 64-65 -1.0915565620001715 1102 66-67 -1.0732404763005903 1102 68-69 -0.9857775336973731 1102 70-71 -1.004698589680558 1102 72-73 -1.0357897946790686 1102 74-75 -1.1374926424891498 1102 76-77 -1.1324659085203024 1102 78-79 -1.09762918855359 1102 80-81 -1.2336039178521254 1102 82-83 -1.3222760012407946 1102 84-85 -1.3346673158956683 1102 86-87 -1.35017888465309 1102 88-89 -1.3637648842820091 1102 90-91 -1.3678953110925818 1102 92-93 -1.5538512872477348 1102 94-95 -1.5286865987019347 1102 96-97 -1.663869005277757 1102 98-99 -1.7207313600296636 1102 100 -1.679268586848636 1103 1 -0.44409955975753945 1103 2 -0.5085741043401235 1103 3 -0.5896256731372844 1103 4 -0.37094233540973676 1103 5 -0.31866061523653855 1103 6 -0.47795001530936787 1103 7 -0.442750164699774 1103 8 -0.4091875799624063 1103 9 -0.6351508639027799 1103 10-11 -0.5944978962149179 1103 12-13 -0.6886013152536918 1103 14-15 -0.7625972251346624 1103 16-17 -0.8075498283754357 1103 18-19 -0.8527176811140507 1103 20-21 -0.8458786417887723 1103 22-23 -0.8252798561081747 1103 24-25 -0.8722191199845923 1103 26-27 -0.8607418405837493 1103 28-29 -0.9117590213782449 1103 30-31 -0.9236253046801508 1103 32-33 -0.9160346274940281 1103 34-35 -0.9045307846017678 1103 36-37 -0.953941018559874 1103 38-39 -1.0464672439509926 1103 40-41 -1.0084991534240473 1103 42-43 -1.000974023134333 1103 44-45 -1.0411293298752682 1103 46-47 -1.1640159105248884 1103 48-49 -1.1117300620358606 1103 50-51 -1.1109972836556992 1103 52-53 -0.8954478074656222 1103 54-55 -0.8639704309282195 1103 56-57 -0.8296655188194677 1103 58-59 -0.8301995288610584 1103 60-61 -0.940647832217735 1103 62-63 -0.8618972702078196 1103 64-65 -0.7856160950900986 1103 66-67 -0.861563202878294 1103 68-69 -0.908521756620317 1103 70-71 -0.9331838661034837 1103 72-73 -0.8685629060268809 1103 74-75 -0.9730811889217144 1103 76-77 -0.9495421386522445 1103 78-79 -0.9331615057349119 1103 80-81 -0.9590467162838543 1103 82-83 -1.0536463089362655 1103 84-85 -1.048470466712775 1103 86-87 -1.0482650508825486 1103 88-89 -1.0621358563036303 1103 90-91 -1.049078032661054 1103 92-93 -1.1688822508943169 1103 94-95 -1.1501835169513477 1103 96-97 -1.243244472244779 1103 98-99 -1.3092710272685402 1103 100 -1.3909448250074803 1104 1 -0.47294985797456945 1104 2 -0.46051698811750796 1104 3 -0.42085405300096923 1104 4 -0.3092282271293598 1104 5 -0.3448249031581554 1104 6 -0.40048410688036284 1104 7 -0.30251417808623415 1104 8 -0.37171691742106105 1104 9 -0.48251909554090133 1104 10-11 -0.40271418772005774 1104 12-13 -0.5514950625680584 1104 14-15 -0.7513851357117645 1104 16-17 -0.7904191193733539 1104 18-19 -0.7979855010101744 1104 20-21 -0.7889964432932146 1104 22-23 -0.7700940499961035 1104 24-25 -0.8224377753751355 1104 26-27 -0.7331245302978999 1104 28-29 -0.7906825400697102 1104 30-31 -0.8325499234465781 1104 32-33 -0.8193678181845527 1104 34-35 -0.8449052149326732 1104 36-37 -0.9582571907480713 1104 38-39 -1.0084043221416863 1104 40-41 -0.9160172338863006 1104 42-43 -0.9372135008273972 1104 44-45 -0.9556655338234705 1104 46-47 -0.9556191503489302 1104 48-49 -0.8904736393732051 1104 50-51 -0.8750211556955279 1104 52-53 -0.6561270214993655 1104 54-55 -0.678052452391178 1104 56-57 -0.6025043885042294 1104 58-59 -0.6913110429522931 1104 60-61 -0.7139644581169904 1104 62-63 -0.6647102301746912 1104 64-65 -0.6830190142035306 1104 66-67 -0.6742174475674787 1104 68-69 -0.6946902932078629 1104 70-71 -0.5920009377797513 1104 72-73 -0.6751600072284987 1104 74-75 -0.8048022089388276 1104 76-77 -0.8437326541883934 1104 78-79 -0.7012690324614255 1104 80-81 -0.8241372357833789 1104 82-83 -0.7729473343776903 1104 84-85 -0.9226631163020151 1104 86-87 -0.9605681779847544 1104 88-89 -0.9095206972025842 1104 90-91 -0.9143336727565732 1104 92-93 -1.0382676676619802 1104 94-95 -1.0369587690603659 1104 96-97 -1.0569403739564471 1104 98-99 -1.1760856901038252 1104 100 -1.0652773155818132 1105 1 -0.42454074206598236 1105 2 -0.44055227303481814 1105 3 -0.4707280495534931 1105 4 -0.2647410873191731 1105 5 -0.391806776671622 1105 6 -0.30813647568589886 1105 7 -0.3279282294042929 1105 8 -0.3611728913497956 1105 9 -0.49939107571833574 1105 10-11 -0.47951429342707996 1105 12-13 -0.48958394237566694 1105 14-15 -0.6074937811516534 1105 16-17 -0.6414743476695648 1105 18-19 -0.6553299748260457 1105 20-21 -0.647048386805352 1105 22-23 -0.6581024286466786 1105 24-25 -0.699925569526286 1105 26-27 -0.7119916886392872 1105 28-29 -0.7294283249387092 1105 30-31 -0.7053012850808003 1105 32-33 -0.8402574226601871 1105 34-35 -0.7947905923506866 1105 36-37 -0.8061457955518136 1105 38-39 -0.7771303859797314 1105 40-41 -0.8429948086412722 1105 42-43 -0.7465328864921332 1105 44-45 -0.7587745378617754 1105 46-47 -0.8534416082827789 1105 48-49 -0.8873170958742591 1105 50-51 -0.7660860424368607 1105 52-53 -0.6547391228362116 1105 54-55 -0.55259715896198 1105 56-57 -0.5464561783299899 1105 58-59 -0.5086577849431251 1105 60-61 -0.5849321313778475 1105 62-63 -0.7576669893784356 1105 64-65 -0.6905821850641516 1105 66-67 -0.6397612774493666 1105 68-69 -0.6917371962057217 1105 70-71 -0.5912817767024379 1105 72-73 -0.5947578740619264 1105 74-75 -0.6370359251503821 1105 76-77 -0.6216634340613751 1105 78-79 -0.6560877610093847 1105 80-81 -0.6338546812152117 1105 82-83 -0.6022504189168671 1105 84-85 -0.6088473595760817 1105 86-87 -0.6116993790862004 1105 88-89 -0.6901902124887478 1105 90-91 -0.7363277269483071 1105 92-93 -0.7894732795813368 1105 94-95 -0.891483055940224 1105 96-97 -0.813981455258169 1105 98-99 -0.8223493364390322 1105 100 -0.9608580629400549 1106 1 -0.45061445232986586 1106 2 -0.4827771005236414 1106 3 -0.5653474962942013 1106 4 -0.37903077775929717 1106 5 -0.3440767361672812 1106 6 -0.43097215002815403 1106 7 -0.471558935627705 1106 8 -0.4370819323701056 1106 9 -0.6175475115903666 1106 10-11 -0.6707857563260191 1106 12-13 -0.6191021957922871 1106 14-15 -0.7879466745911898 1106 16-17 -0.7713813669623093 1106 18-19 -0.7861063247372684 1106 20-21 -0.9222368831963337 1106 22-23 -0.8629296600762473 1106 24-25 -0.8381287909856212 1106 26-27 -0.8650401904337528 1106 28-29 -0.871714743655005 1106 30-31 -1.0117792828536096 1106 32-33 -1.0241997379429293 1106 34-35 -0.9939338845011534 1106 36-37 -0.977518916716889 1106 38-39 -1.0457388080651882 1106 40-41 -1.0132779896207396 1106 42-43 -1.056880190050265 1106 44-45 -1.1586737369741655 1106 46-47 -1.0162954933086823 1106 48-49 -1.0518971009369622 1106 50-51 -1.0756609576075888 1106 52-53 -0.9398505294780648 1106 54-55 -0.881050171673202 1106 56-57 -0.9318022266351988 1106 58-59 -0.8700996033360369 1106 60-61 -0.8167049413852112 1106 62-63 -0.9283652308370733 1106 64-65 -0.8189909784325735 1106 66-67 -0.7616490998918906 1106 68-69 -0.8806912786915149 1106 70-71 -0.9145905295855599 1106 72-73 -0.8864271161873489 1106 74-75 -0.803868191548947 1106 76-77 -0.9215853863322181 1106 78-79 -0.9466263465323976 1106 80-81 -0.9203815327970979 1106 82-83 -0.9387339914627368 1106 84-85 -0.9410222764869474 1106 86-87 -0.9180622855740239 1106 88-89 -0.8970892116669873 1106 90-91 -1.0179157247546513 1106 92-93 -0.980428640793729 1106 94-95 -1.0195636482528379 1106 96-97 -1.05874836476114 1106 98-99 -0.9931974605337892 1106 100 -1.0918844211667604 1107 1 -0.5968823215404946 1107 2 -0.5303240241080971 1107 3 -0.5421529192646091 1107 4 -0.4156811358970174 1107 5 -0.4584685128562853 1107 6 -0.44658459028037356 1107 7 -0.5783779109563341 1107 8 -0.5676257586410145 1107 9 -0.6783659529331416 1107 10-11 -0.6403542640169348 1107 12-13 -0.7455530196161675 1107 14-15 -0.8211460069043852 1107 16-17 -0.8868179212901239 1107 18-19 -0.9283876708225947 1107 20-21 -0.8717908680902156 1107 22-23 -0.8969007424218347 1107 24-25 -0.9228422879270255 1107 26-27 -0.9722522646131537 1107 28-29 -0.9687993573852367 1107 30-31 -0.9903824292981156 1107 32-33 -0.9740604023987913 1107 34-35 -1.0029564872349752 1107 36-37 -0.9268806587021459 1107 38-39 -0.9891013443610674 1107 40-41 -0.9286067354412566 1107 42-43 -0.9160435775405702 1107 44-45 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52-53 1.303822414126948 2311 54-55 1.2356637543270281 2311 56-57 1.2715474347961049 2311 58-59 1.2974163384581345 2311 60-61 1.2827397190302605 2311 62-63 1.191724140237092 2311 64-65 1.1973427224825812 2311 66-67 1.2461329935889793 2311 68-69 1.19511805860337 2311 70-71 1.1903171201976903 2311 72-73 1.1807379114407297 2311 74-75 1.185984758044242 2311 76-77 1.1327286363673537 2311 78-79 1.2274189984380826 2311 80-81 1.197051471731914 2311 82-83 1.2251655043680998 2311 84-85 1.236671005443302 2311 86-87 1.334498424511196 2311 88-89 1.4169987745507946 2311 90-91 1.3914227108972206 2311 92-93 1.4200242440484914 2311 94-95 1.5121492177848346 2311 96-97 1.515874423109814 2311 98-99 1.5192857608782262 2311 100 1.7067579654047869 2312 1 0.5100379247944886 2312 2 0.5905693410243273 2312 3 0.6738281733332663 2312 4 0.5194998957456889 2312 5 0.5812294193660534 2312 6 0.45745865628479976 2312 7 0.5848851809082731 2312 8 0.6694364272912807 2312 9 0.8013423353756721 2312 10-11 0.8487631835288312 2312 12-13 0.8951617713166726 2312 14-15 1.1011922385977968 2312 16-17 1.0682913632396023 2312 18-19 1.0241298747062402 2312 20-21 1.0522186121410755 2312 22-23 1.0983261489780602 2312 24-25 1.1084312679327368 2312 26-27 1.1394429317059078 2312 28-29 1.1657680804244421 2312 30-31 1.0742337275983758 2312 32-33 1.1939993606297534 2312 34-35 1.1928159079092993 2312 36-37 1.2248940564429063 2312 38-39 1.2790536289453058 2312 40-41 1.2720523799063983 2312 42-43 1.3964842085860028 2312 44-45 1.373027149230488 2312 46-47 1.360968211577159 2312 48-49 1.3580126801547223 2312 50-51 1.2617707202672293 2312 52-53 1.1234559414577703 2312 54-55 1.1128201175107577 2312 56-57 1.0714618218185734 2312 58-59 1.1769237770580077 2312 60-61 1.109419029371935 2312 62-63 1.0795444943199186 2312 64-65 1.0467227623416946 2312 66-67 1.0962095988176301 2312 68-69 1.1256795941959652 2312 70-71 1.1430387414516403 2312 72-73 1.1043482432385048 2312 74-75 1.154665825791561 2312 76-77 1.0792690853823785 2312 78-79 1.0877019213379313 2312 80-81 0.8974998995998966 2312 82-83 1.154765209054787 2312 84-85 1.2435316021344498 2312 86-87 1.2466428281298008 2312 88-89 1.2841844557885338 2312 90-91 1.2971061051715829 2312 92-93 1.3778354223370464 2312 94-95 1.360576659215429 2312 96-97 1.3678859695179995 2312 98-99 1.2385294228755406 2312 100 1.363900656506189 2313 1 0.5802803126785072 2313 2 0.5969440424116037 2313 3 0.6687763880054653 2313 4 0.5306339527944957 2313 5 0.5253249250516561 2313 6 0.5789259655668246 2313 7 0.6304538506138826 2313 8 0.6573011826763775 2313 9 0.7720486334588088 2313 10-11 0.6587118307008737 2313 12-13 0.7913911700726217 2313 14-15 0.9861901665925572 2313 16-17 0.9910036268879381 2313 18-19 0.966570625090263 2313 20-21 1.0149460421918093 2313 22-23 1.0703694469467706 2313 24-25 1.0804739654011826 2313 26-27 1.0926645961021748 2313 28-29 1.1523581413182384 2313 30-31 1.102827546450797 2313 32-33 1.1472260943655073 2313 34-35 1.159270105477404 2313 36-37 1.2037479072737796 2313 38-39 1.192635998212694 2313 40-41 1.1934779669882758 2313 42-43 1.2532096691634251 2313 44-45 1.1605317357258684 2313 46-47 1.187385564683133 2313 48-49 1.3096590857095336 2313 50-51 1.240962401377793 2313 52-53 1.105493868161041 2313 54-55 1.0764429004463096 2313 56-57 1.0220073427973873 2313 58-59 1.0907403484131777 2313 60-61 1.0727265426832702 2313 62-63 1.0380027095979258 2313 64-65 1.076280349214322 2313 66-67 1.0251295409874999 2313 68-69 1.0360570167152972 2313 70-71 1.0512390223125934 2313 72-73 1.0207205507244765 2313 74-75 1.0577613446323113 2313 76-77 1.0617485124526098 2313 78-79 1.0508633873976123 2313 80-81 1.1045396075545533 2313 82-83 1.0531345324175092 2313 84-85 1.1199409448461282 2313 86-87 1.1570191194742065 2313 88-89 1.2143583961380777 2313 90-91 1.2777682997218847 2313 92-93 1.326863995843631 2313 94-95 1.4195862496516227 2313 96-97 1.4364424528945712 2313 98-99 1.4259638513882287 2313 100 1.5640935511579706 2314 1 0.5098947737872415 2314 2 0.5221440591732929 2314 3 0.5485709857653269 2314 4 0.4908473585782218 2314 5 0.5106168958288606 2314 6 0.5073089142026035 2314 7 0.5504630565321449 2314 8 0.5591526607813719 2314 9 0.67913242005433 2314 10-11 0.721301422657298 2314 12-13 0.48518015407632475 2314 14-15 0.8407042134241252 2314 16-17 0.9026865779252091 2314 18-19 0.9251542927764547 2314 20-21 0.9589603765795829 2314 22-23 0.7613612022235188 2314 24-25 0.8218965697956762 2314 26-27 0.9522840732491673 2314 28-29 1.0186364729491117 2314 30-31 0.9666790653215998 2314 32-33 1.047648139802149 2314 34-35 1.1087558273865739 2314 36-37 1.1384658064856126 2314 38-39 1.160370100371928 2314 40-41 1.164896415112885 2314 42-43 1.227250424352306 2314 44-45 1.3059830623468258 2314 46-47 1.3090984617886434 2314 48-49 1.337248109275592 2314 50-51 1.261763727177474 2314 52-53 1.1606420863182123 2314 54-55 1.1057928635212662 2314 56-57 1.1142523974894445 2314 58-59 1.0963459459931215 2314 60-61 1.1568242079242737 2314 62-63 1.1333578381135112 2314 64-65 1.0963540838383068 2314 66-67 1.1307652033552742 2314 68-69 1.1460705724326488 2314 70-71 1.1010753112487563 2314 72-73 1.1103896613342101 2314 74-75 1.1635303225959461 2314 76-77 1.1056806430101176 2314 78-79 1.1650728268627972 2314 80-81 1.2184721701646275 2314 82-83 1.0849319757612932 2314 84-85 1.125448734287879 2314 86-87 1.3049283617618634 2314 88-89 1.4318091569165254 2314 90-91 1.482334406991594 2314 92-93 1.5570529350916722 2314 94-95 1.576170207794238 2314 96-97 1.5611832145723668 2314 98-99 1.6604620407518311 2314 100 1.5957535182992046 2315 1 0.42208876814996543 2315 2 0.46018011810080495 2315 3 0.495625956037685 2315 4 0.40419276232083234 2315 5 0.38199589139605195 2315 6 -0.32653944039258676 2315 7 0.1659282075536055 2315 8 0.31501462042124473 2315 9 0.5621911732178404 2315 10-11 0.6158150537391194 2315 12-13 0.6552627438635099 2315 14-15 0.8014500359520511 2315 16-17 0.6773298320015115 2315 18-19 0.798633477339429 2315 20-21 0.8410005908983251 2315 22-23 0.7350664567334491 2315 24-25 0.818816620376694 2315 26-27 0.8745653873656067 2315 28-29 0.9554357815651571 2315 30-31 0.9839456207792736 2315 32-33 1.044829224480722 2315 34-35 1.0900461316855896 2315 36-37 1.1148542247899442 2315 38-39 1.1405089208992578 2315 40-41 1.2550011682490805 2315 42-43 1.2370771766480004 2315 44-45 1.3012101988018259 2315 46-47 1.313132526745143 2315 48-49 1.3330198005409244 2315 50-51 1.237453599625276 2315 52-53 1.1495348480898073 2315 54-55 0.9434242377665711 2315 56-57 0.9503406691878098 2315 58-59 0.8701122626530875 2315 60-61 0.8516448742936475 2315 62-63 1.093892732809202 2315 64-65 1.1400690985536812 2315 66-67 1.0839067491927281 2315 68-69 1.1244547065802948 2315 70-71 1.1211644272265389 2315 72-73 1.1480425009980877 2315 74-75 1.189520386284812 2315 76-77 1.1832773871916942 2315 78-79 1.0741373539128034 2315 80-81 1.0593573339625486 2315 82-83 1.2419254889307396 2315 84-85 1.2231769842530156 2315 86-87 1.2924774248511213 2315 88-89 1.4182279717143267 2315 90-91 1.425569161708946 2315 92-93 1.544605494595988 2315 94-95 1.690472134663846 2315 96-97 1.6797141940947355 2315 98-99 1.7973011219345345 2315 100 1.8524709296481774 2316 1 0.015407947783081966 2316 2 -0.0763090464427627 2316 3 0.04169954708034851 2316 4 0.06281142641841342 2316 5 0.10682719695977028 2316 6 0.20096322781713383 2316 7 0.15003819771282423 2316 8 0.22281775037058082 2316 9 0.33022833636208304 2316 10-11 0.20385750358868648 2316 12-13 0.2673272382668799 2316 14-15 0.43917147642684284 2316 16-17 0.512068104111016 2316 18-19 0.4772985338787876 2316 20-21 0.4417094228229601 2316 22-23 0.30708651324533776 2316 24-25 0.3818866419447744 2316 26-27 0.440252511222603 2316 28-29 0.5496804420712778 2316 30-31 0.5725510373693012 2316 32-33 0.6281570151245717 2316 34-35 0.6896037675013318 2316 36-37 0.7231507282381386 2316 38-39 0.7534434518372777 2316 40-41 0.6727227055355982 2316 42-43 0.6760974641997564 2316 44-45 0.5912618844902582 2316 46-47 0.5569534527833326 2316 48-49 0.6901242965037468 2316 50-51 0.4982698245382906 2316 52-53 0.2805574206600099 2316 54-55 0.24001391381622028 2316 56-57 0.3437128794074127 2316 58-59 0.36726207958734136 2316 60-61 0.42874506736762896 2316 62-63 0.5121615703436788 2316 64-65 0.5314743168628198 2316 66-67 0.548122943125982 2316 68-69 0.5108084066591232 2316 70-71 0.4487169861829159 2316 72-73 0.5165935970507576 2316 74-75 0.63141702877558 2316 76-77 0.7139974492768033 2316 78-79 0.7562416601769684 2316 80-81 0.8429546536906081 2316 82-83 0.8446214600714228 2316 84-85 0.9866823184484126 2316 86-87 1.002817990194984 2316 88-89 1.0038564538294104 2316 90-91 1.0811423708685943 2316 92-93 1.2062750625618754 2316 94-95 1.3515079848317342 2316 96-97 1.4458650330468537 2316 98-99 1.5163247900471823 2316 100 1.3806019321388554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 38.0 1 419.0 2 194.0 3 132.0 4 106.0 5 118.0 6 136.0 7 731.0 8 2523.0 9 4185.0 10 5975.0 11 7970.0 12 10169.0 13 12573.0 14 14784.0 15 18591.0 16 24411.0 17 32468.0 18 40391.0 19 49487.0 20 59897.0 21 70255.0 22 76564.0 23 78362.0 24 78568.0 25 82874.0 26 91921.0 27 107017.0 28 125853.0 29 150197.0 30 182171.0 31 223110.0 32 282162.0 33 361901.0 34 491476.0 35 658313.0 36 936492.0 37 1330965.0 38 1215421.0 39 314849.0 40 369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.59582359559073 18.40729341723924 12.087528862528792 23.909354124641236 2 16.615168779523906 20.55791823120528 39.64040166950159 23.186511319769217 3 18.982089541790486 26.431272073055904 30.21850268989391 24.368135695259696 4 12.881081853005085 16.698452655630476 37.85243666136511 32.56802882999933 5 13.012944954366079 37.357719963064426 34.427011977460616 15.202323105108887 6 29.975801683042103 36.51192423257387 19.071153247697602 14.44112083668643 7 26.63492300930544 33.04629021916065 22.78982824754114 17.528958523992767 8 25.92522877574515 34.540487774818004 21.030212123503887 18.504071325932962 9 27.515000091722268 15.51468865611193 20.559861523180597 36.4104497289852 10-11 24.91780179474192 25.58697549674967 29.00855499903304 20.486667709475366 12-13 25.429568495175914 23.885931707489213 28.33890006015188 22.345599737182997 14-15 23.073554899729366 25.333635182319377 26.43352892615613 25.159280991795125 16-17 22.24716741544247 27.672100599627313 27.27642777241882 22.804304212511394 18-19 21.802742105280473 27.14938749829986 29.207873974850045 21.83999642156962 20-21 22.7898909440518 26.70817532143563 28.804933380366176 21.697000354146393 22-23 22.645719536524584 26.474927750854455 28.643942237646446 22.235410474974515 24-25 22.194539063506845 26.260584573014363 29.0322987115419 22.512577651936894 26-27 22.10567159711558 26.39370682625472 28.858396940606756 22.642224636022938 28-29 21.935010844219498 27.34790844780904 28.642382680441298 22.07469802753017 30-31 22.99403026823823 26.583436314857884 28.79526526253394 21.627268154369947 32-33 22.665355035728922 26.62322379226835 28.555443219387495 22.15597795261523 34-35 22.756692907156914 26.791881472483333 28.722342318161758 21.729083302197996 36-37 22.45547267063448 26.60165165306017 28.654562067479954 22.288313608825394 38-39 22.40262801812244 26.71170184488681 28.734021601792612 22.151648535198138 40-41 22.431005294272193 26.132088475467043 29.150832398590854 22.28607383166991 42-43 22.147048645422927 26.52444571767896 28.49537354509445 22.833132091803662 44-45 21.57637328406445 26.842734596786737 28.97203433418459 22.608857784964222 46-47 21.501554043309877 26.320667239097766 28.740576190203782 23.437202527388575 48-49 21.782847670258175 26.4141987921076 28.749212842269205 23.053740695365022 50-51 21.67383521628232 26.6685937438087 28.66923025332827 22.98834078658071 52-53 21.453131993119122 26.93002385677644 28.305289437760923 23.311554712343515 54-55 21.154917556151403 26.608811211481186 28.808431786681904 23.427839445685507 56-57 21.744694515210384 26.465233012253897 28.280694398698653 23.509378073837066 58-59 21.39238432274865 26.56200087806948 28.5545603945851 23.49105440459677 60-61 21.84831116980037 26.493569916408877 28.310086119709887 23.348032794080865 62-63 21.821834484596163 26.298237826157784 28.279922956830216 23.60000473241584 64-65 21.52728851054297 26.09976617848842 28.29596622889306 24.07697908207555 66-67 21.61240050659462 26.178956160242407 27.929508016530836 24.27913531663214 68-69 21.33145473166494 26.379489579333814 28.119534572795956 24.169521116205292 70-71 21.500830212088296 26.29019649578032 28.10813516826017 24.100838123871217 72-73 21.694619537910093 26.701545789158526 27.698787331878066 23.90504734105332 74-75 22.11483567576203 26.76240098293728 27.372291414777088 23.750471926523602 76-77 22.128779049682933 26.597211493640888 27.313773781079355 23.960235675596824 78-79 22.092176660581085 26.771296873084836 27.51534981506302 23.621176651271057 80-81 22.291194381410854 26.75260298245339 27.317182204291562 23.639020431844198 82-83 22.722696838180596 26.622349006335234 27.401034808315178 23.253919347169 84-85 22.97603123506021 26.879825793703287 26.798651315121745 23.345491656114756 86-87 22.747218071785348 26.99067093163429 27.024395515876243 23.23771548070412 88-89 22.96803061955143 26.339523123880817 27.053211179745524 23.63923507682223 90-91 23.649064968560214 26.24980382071152 26.87540749640787 23.225723714320395 92-93 23.8339093767321 26.305295861181733 26.728336701111665 23.132458060974496 94-95 23.64258526013311 26.19210246315957 26.725873259677797 23.43943901702952 96-97 23.85466942720083 26.000248518079687 26.60545654696379 23.53962550775569 98-99 23.9999857182572 26.0630114692896 26.271825950920793 23.665176861532412 100 24.48941101751515 25.97535776162741 26.103640329395805 23.431590891461635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2454.0 1 1784.5 2 2718.0 3 3900.5 4 2617.0 5 1946.0 6 2649.0 7 4230.5 8 6426.5 9 7562.5 10 6641.0 11 5594.5 12 5820.5 13 6443.5 14 7159.5 15 7886.0 16 8592.0 17 9448.5 18 10349.5 19 11371.5 20 12591.0 21 14081.0 22 16130.0 23 19061.0 24 22631.0 25 26917.0 26 32589.5 27 39119.5 28 47068.5 29 57699.0 30 69456.0 31 80927.5 32 95201.0 33 109053.5 34 121924.5 35 135934.5 36 151156.5 37 170240.5 38 182955.5 39 187733.5 40 192706.5 41 198434.0 42 206457.5 43 219592.5 44 233545.0 45 252035.0 46 272783.5 47 290144.5 48 308892.0 49 321858.0 50 325043.0 51 324180.0 52 314836.0 53 297493.0 54 277824.0 55 249564.5 56 220508.5 57 190831.5 58 159528.5 59 132240.0 60 105008.5 61 81893.0 62 64520.5 63 50042.5 64 37050.5 65 26479.0 66 19409.5 67 14889.5 68 11505.5 69 8824.0 70 6958.5 71 5413.5 72 4215.0 73 3295.0 74 2533.0 75 1869.5 76 1366.0 77 965.0 78 655.5 79 477.5 80 350.0 81 247.0 82 187.5 83 134.5 84 98.5 85 90.0 86 70.5 87 50.0 88 38.5 89 30.5 90 25.5 91 20.5 92 15.5 93 19.5 94 18.5 95 11.5 96 7.0 97 5.0 98 5.0 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21721304935598948 2 0.1471416145656761 3 0.02327782582027391 4 0.02208803917281553 5 0.027617047711004462 6 0.055598030161231486 7 0.06220484542711801 8 0.055402065301650115 9 0.04220243225984716 10-11 0.04433005073530215 12-13 0.04265735068387537 14-15 0.020905251270342203 16-17 0.019148566279094833 18-19 0.01877763279488722 20-21 0.022556955086813834 22-23 0.025741384055011255 24-25 0.022514962616903535 26-27 0.019799449562704415 28-29 0.019491504783362246 30-31 0.017790809751995273 32-33 0.026385268593635793 34-35 0.02703615187724537 36-37 0.025741384055011255 38-39 0.024096678983524675 40-41 0.025146490731282063 42-43 0.031109421458544055 44-45 0.04083067824277751 46-47 0.045484843657835276 48-49 0.04113862302211967 50-51 0.02884882682837314 52-53 0.044651993004614414 54-55 0.042909305503337145 56-57 0.045155902643537964 58-59 0.04861328266615231 60-61 0.025370450570803643 62-63 0.026714209607933106 64-65 0.027127135562051017 66-67 0.03265614410023994 68-69 0.03717733336058178 70-71 0.04045974475856989 72-73 0.044651993004614414 74-75 0.02619630247903946 76-77 0.018945602674528404 78-79 0.018189738216143083 80-81 0.021864079333293956 82-83 0.020471329081269148 84-85 0.012359783643597029 86-87 0.013731537660666688 88-89 0.019337532393691164 90-91 0.020114393087031633 92-93 0.020254367986732618 94-95 0.02444661623277714 96-97 0.02520248069116246 98-99 0.030129597160637157 100 0.02928274901744619 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7144138.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.3052067762344 #Duplication Level Percentage of deduplicated Percentage of total 1 77.64705324645446 49.93109814578536 2 14.071721319972724 18.097698983567845 3 4.09687245923152 7.903506918802286 4 1.5646143242776915 4.024513905909409 5 0.7887993244171251 2.5361951830798612 6 0.4687691319570065 1.8086577576486726 7 0.28942080205829046 1.3027885165191382 8 0.19193883762702021 0.9874133313596491 9 0.1504221574131707 0.8705635142563098 >10 0.6886953384885599 7.53669675430626 >50 0.027837505546582077 1.2057720324836403 >100 0.011965261018489668 1.4286468179468204 >500 0.0011846012043568539 0.5070691745369607 >1k 6.048774284545868E-4 0.7907226745371566 >5k 2.520322617105634E-5 0.10160159964474177 >10k+ 7.560967851316902E-5 0.9670546896159032 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26314 0.36832995107317357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20871 0.29214161316592707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12484 0.17474466478671044 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7994979940197123E-5 2 0.0 0.0 0.0 0.0 2.7994979940197123E-5 3 0.0 0.0 0.0 2.7994979940197123E-5 2.7994979940197123E-5 4 0.0 0.0 0.0 4.199246991029569E-5 2.7994979940197123E-5 5 4.199246991029569E-5 0.0 0.0 4.199246991029569E-5 2.7994979940197123E-5 6 5.598995988039425E-5 0.0 0.0 4.199246991029569E-5 4.199246991029569E-5 7 5.598995988039425E-5 0.0 0.0 4.199246991029569E-5 5.598995988039425E-5 8 5.598995988039425E-5 0.0 0.0 5.598995988039425E-5 5.598995988039425E-5 9 5.598995988039425E-5 0.0 0.0 6.998744985049281E-5 5.598995988039425E-5 10-11 5.598995988039425E-5 0.0 0.0 9.098368480564065E-5 6.298870486544353E-5 12-13 5.598995988039425E-5 0.0 0.0 1.539723896710842E-4 8.398493982059137E-5 14-15 5.598995988039425E-5 0.0 0.0 1.7496862462623204E-4 1.3297615471593633E-4 16-17 9.798242979068993E-5 0.0 0.0 2.7295105441692193E-4 1.819673696112813E-4 18-19 9.798242979068993E-5 0.0 0.0 3.9192971916275973E-4 1.819673696112813E-4 20-21 9.798242979068993E-5 0.0 0.0 7.768606933404702E-4 2.169610945365277E-4 22-23 9.798242979068993E-5 0.0 0.0 0.0027505067791243672 2.9394728937206984E-4 24-25 1.0498117477573921E-4 0.0 0.0 0.008328506532208645 3.219422693122669E-4 26-27 1.119799197607885E-4 0.0 0.0 0.016328072050119973 3.359397592823655E-4 28-29 1.119799197607885E-4 0.0 0.0 0.04305627914802318 3.359397592823655E-4 30-31 1.119799197607885E-4 0.0 0.0 0.12958176339818744 3.359397592823655E-4 32-33 1.119799197607885E-4 0.0 0.0 0.3185898704644283 4.059272091328583E-4 34-35 1.1897866474583777E-4 0.0 0.0 0.5977348141931189 4.1992469910295685E-4 36-37 1.2597740973088707E-4 1.3997489970098562E-5 0.0 1.0232865042640553 4.1992469910295685E-4 38-39 1.2597740973088707E-4 2.7994979940197123E-5 0.0 1.6334650870405918 4.409209340581047E-4 40-41 1.539723896710842E-4 2.7994979940197123E-5 0.0 2.2955113689013285 4.689159139983018E-4 42-43 1.6796987964118275E-4 4.199246991029569E-5 0.0 2.900118950669766 4.899121489534497E-4 44-45 1.6796987964118275E-4 4.199246991029569E-5 0.0 3.605536455202853 5.179071288936468E-4 46-47 1.6796987964118275E-4 4.199246991029569E-5 0.0 4.403736322002739 5.389033638487946E-4 48-49 1.6796987964118275E-4 4.199246991029569E-5 0.0 5.210341681529668 5.73897088774041E-4 50-51 1.6796987964118275E-4 4.199246991029569E-5 0.0 6.014168539297533 6.298870486544353E-4 52-53 1.6796987964118275E-4 4.199246991029569E-5 0.0 6.781706904317918 6.578820285946323E-4 54-55 1.6796987964118275E-4 4.199246991029569E-5 0.0 7.6262804553887396 6.71879518564731E-4 56-57 1.6796987964118275E-4 4.899121489534497E-5 0.0 8.533079848121634 6.928757535198788E-4 58-59 1.6796987964118275E-4 5.598995988039425E-5 0.0 9.515941601352045 7.138719884750267E-4 60-61 1.7496862462623204E-4 5.598995988039425E-5 0.0 10.468211840252806 7.138719884750267E-4 62-63 1.819673696112813E-4 5.598995988039425E-5 0.0 11.435536379616408 7.558644583853223E-4 64-65 1.819673696112813E-4 5.598995988039425E-5 0.0 12.35414265513908 7.558644583853223E-4 66-67 1.819673696112813E-4 5.598995988039425E-5 0.0 13.34128764030034 7.628632033703717E-4 68-69 1.819673696112813E-4 5.598995988039425E-5 0.0 14.400568690022507 7.698619483554209E-4 70-71 2.0996234955147843E-4 5.598995988039425E-5 0.0 15.482595660946078 7.768606933404702E-4 72-73 2.0996234955147843E-4 5.598995988039425E-5 0.0 16.474968708611172 8.048556732806673E-4 74-75 2.0996234955147843E-4 5.598995988039425E-5 0.0 17.434426658611578 8.328506532208644E-4 76-77 2.0996234955147843E-4 5.598995988039425E-5 0.0 18.46422199571173 8.538468881760122E-4 78-79 2.23959839521577E-4 6.998744985049281E-5 0.0 19.5680640547537 8.538468881760122E-4 80-81 2.23959839521577E-4 6.998744985049281E-5 0.0 20.626344283943006 8.538468881760122E-4 82-83 2.23959839521577E-4 6.998744985049281E-5 0.0 21.66854139715666 8.678443781461109E-4 84-85 2.23959839521577E-4 6.998744985049281E-5 0.0 22.669158966414145 8.818418681162094E-4 86-87 2.23959839521577E-4 8.398493982059137E-5 0.0 23.683676043211932 9.168355930414557E-4 88 2.23959839521577E-4 8.398493982059137E-5 0.0 24.449303750851396 9.518293179667022E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11965 0.0 50.48272 1 TACCTGG 6430 0.0 45.08778 2 GTATCAA 21310 0.0 40.416637 1 ACCTGGG 8225 0.0 34.40417 3 ATCAACG 25655 0.0 33.163246 3 CAACGCA 26045 0.0 32.792763 5 TATCAAC 26410 0.0 32.39773 2 TCAACGC 27040 0.0 31.60324 4 AACGCAG 27755 0.0 31.195997 6 GTACATG 22460 0.0 30.585474 1 TACATGG 22915 0.0 29.267399 2 ACATGGG 22825 0.0 28.234308 3 ACGCAGA 30470 0.0 28.00037 7 CGCAGAG 31075 0.0 27.34666 8 CATGGGG 18065 0.0 26.930645 4 CCTGGGG 8155 0.0 26.514183 4 ATGGGGG 14145 0.0 26.35225 5 GTACCTG 11285 0.0 25.927313 1 ATAACGC 655 0.0 25.117548 3 GTACTAG 2295 0.0 25.04636 1 >>END_MODULE