FastQCFastQC Report
Mon 6 Jun 2016
ERR1211381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7144138
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246080.34445023318418544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206910.2896220649713093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125720.17597644390407913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116750.051.9869421
TACCTGG63850.042.686882
GTATCAA210700.040.364441
ACCTGGG77100.034.4983633
ATCAACG256600.032.3422663
CAACGCA259900.031.9811535
TATCAAC266150.031.5695442
TCAACGC270200.030.8711264
AACGCAG278450.030.289556
GTACATG226050.028.0356311
TACATGG227250.027.4820022
CCTGGGG72650.027.1677154
ACGCAGA309550.026.8213967
CGCAGAG314000.026.3963898
ACATGGG226850.025.8530753
TGGGGGG137900.024.8392726
CATGGGG174550.024.688174
ATGGGGG133850.023.8354825
GTACCTG116500.023.7691861
GCAGAGT341750.023.7302239
Produced by FastQC (version 0.11.5)