##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211381_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7144138 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06745138461771 33.0 31.0 34.0 30.0 34.0 2 32.19752334011465 33.0 31.0 34.0 30.0 34.0 3 32.27467456535694 34.0 31.0 34.0 30.0 34.0 4 35.92211572620798 37.0 35.0 37.0 35.0 37.0 5 35.77744312889813 37.0 35.0 37.0 35.0 37.0 6 35.8163102392479 37.0 35.0 37.0 35.0 37.0 7 35.74637458011029 37.0 35.0 37.0 33.0 37.0 8 35.73214753130468 37.0 35.0 37.0 33.0 37.0 9 37.32051914450701 39.0 37.0 39.0 34.0 39.0 10-11 37.2962943185028 39.0 37.0 39.0 34.0 39.0 12-13 37.20969128815821 39.0 37.0 39.0 33.0 39.0 14-15 38.41326798278533 40.0 38.0 41.0 33.5 41.0 16-17 38.39567418770466 40.0 38.0 41.0 33.5 41.0 18-19 38.40129166037946 40.0 38.0 41.0 33.5 41.0 20-21 38.3593291450977 40.0 38.0 41.0 34.0 41.0 22-23 38.25817523401703 40.0 38.0 41.0 34.0 41.0 24-25 38.13726246609458 40.0 37.5 41.0 33.0 41.0 26-27 38.00440724969199 40.0 37.0 41.0 33.0 41.0 28-29 37.91752769893303 40.0 37.0 41.0 33.0 41.0 30-31 37.95696800649708 40.0 37.0 41.0 33.0 41.0 32-33 37.78344581249691 40.0 37.0 41.0 33.0 41.0 34-35 37.665237709573915 40.0 37.0 41.0 33.0 41.0 36-37 37.51394660909406 40.0 36.0 41.0 32.0 41.0 38-39 37.394507146978405 40.0 36.0 41.0 32.0 41.0 40-41 37.31674640103537 40.0 36.0 41.0 32.0 41.0 42-43 37.174643043009524 40.0 36.0 41.0 31.0 41.0 44-45 37.02012636094096 39.0 36.0 41.0 31.0 41.0 46-47 36.797523718046875 39.0 35.0 41.0 30.5 41.0 48-49 36.57325943311845 39.0 35.0 41.0 30.0 41.0 50-51 36.38917669283544 39.0 35.0 41.0 29.5 41.0 52-53 36.18225347830627 39.0 35.0 40.0 29.0 41.0 54-55 35.97399679289509 38.5 35.0 40.0 28.5 41.0 56-57 35.744199510143844 38.0 35.0 40.0 28.0 41.0 58-59 35.478526170687076 38.0 34.0 40.0 28.0 41.0 60-61 35.216010664967555 37.5 34.0 40.0 27.0 41.0 62-63 34.911314913009804 37.0 34.0 40.0 26.5 41.0 64-65 34.55704928992134 36.5 33.5 39.5 26.0 41.0 66-67 34.199817038808604 36.0 33.0 39.0 26.0 40.5 68-69 33.824751635536714 35.5 33.0 39.0 25.5 40.0 70-71 33.44634972056811 35.0 32.5 38.0 25.0 40.0 72-73 33.08258785034667 35.0 32.0 37.5 24.5 39.5 74-75 32.711814217474526 35.0 32.0 37.0 24.0 39.0 76-77 31.688469063727492 34.0 30.5 36.0 22.5 38.0 78-79 32.17230980140641 34.5 31.0 36.0 24.0 38.0 80-81 32.06762803294113 35.0 31.5 35.5 24.0 37.0 82-83 31.802485268341684 34.0 31.0 35.0 23.5 37.0 84-85 31.541366502158834 34.0 31.0 35.0 23.0 36.5 86-87 31.299960051163623 34.0 31.0 35.0 22.5 36.0 88-89 31.081123292971103 34.0 31.0 35.0 20.5 36.0 90-91 30.86823224859318 34.0 30.5 35.0 20.0 35.0 92-93 30.69535050974659 34.0 30.0 35.0 20.0 35.0 94-95 30.543089803136503 34.0 30.0 35.0 20.0 35.0 96-97 30.385420816339213 34.0 30.0 35.0 19.0 35.0 98-99 30.191260093240082 34.0 29.0 35.0 19.0 35.0 100 28.296518768254476 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2856727047829999 1101 2 -0.2453707556067215 1101 3 -0.262545955611138 1101 4 -0.14052059759747948 1101 5 -0.2069725662546773 1101 6 -0.2201807901765136 1101 7 -0.2908124763928299 1101 8 -0.3066955739197823 1101 9 -0.43480726127501157 1101 10-11 -0.4214249102013383 1101 12-13 -0.43892535940855737 1101 14-15 -0.5755598766132124 1101 16-17 -0.5613395180711436 1101 18-19 -0.5796179694789032 1101 20-21 -0.644282244117484 1101 22-23 -0.7149405197343341 1101 24-25 -0.7326379031196382 1101 26-27 -0.7307058249348302 1101 28-29 -0.7690900423232279 1101 30-31 -0.7346007779036867 1101 32-33 -0.613278463329813 1101 34-35 -0.659745841268446 1101 36-37 -0.647440838015747 1101 38-39 -0.6424161292131316 1101 40-41 -0.6185541240423262 1101 42-43 -0.6124183248549571 1101 44-45 -0.6637474249304205 1101 46-47 -0.6965574998644541 1101 48-49 -0.6202698712545214 1101 50-51 -0.6976301716838265 1101 52-53 -0.577054947014588 1101 54-55 -0.636678476121503 1101 56-57 -0.6275230356444794 1101 58-59 -0.606114652942658 1101 60-61 -0.49812767729816443 1101 62-63 -0.6217785214765215 1101 64-65 -0.6550645723439459 1101 66-67 -0.7544011615061237 1101 68-69 -0.8074654806434793 1101 70-71 -0.8338331958943428 1101 72-73 -0.8990832182219393 1101 74-75 -0.9246811745829433 1101 76-77 -0.7641837475589668 1101 78-79 -0.6709695704968546 1101 80-81 -0.6411551325633198 1101 82-83 -0.692972446297258 1101 84-85 -0.7162213193162046 1101 86-87 -0.7765540343406236 1101 88-89 -0.8887504620926521 1101 90-91 -0.8645610964976633 1101 92-93 -1.0121640498976738 1101 94-95 -1.0713377656981855 1101 96-97 -0.9621122489579292 1101 98-99 -1.0852102271786563 1101 100 -1.066009966415912 1102 1 -0.2578966266855076 1102 2 -0.26853159303790974 1102 3 -0.2922044119958045 1102 4 -0.19666993429867574 1102 5 -0.1793778982604337 1102 6 -0.23279402510566172 1102 7 -0.2855631744864482 1102 8 -0.2602827030609518 1102 9 -0.43206822017553037 1102 10-11 -0.42246658737043674 1102 12-13 -0.46089241059199537 1102 14-15 -0.5794668994209999 1102 16-17 -0.6105049010767303 1102 18-19 -0.5423627285290422 1102 20-21 -0.6219387100920457 1102 22-23 -0.6693324750979244 1102 24-25 -0.7103361353278785 1102 26-27 -0.7728630662310323 1102 28-29 -0.7927343406731353 1102 30-31 -0.6226138903935947 1102 32-33 -0.6909825293995908 1102 34-35 -0.6531189840810185 1102 36-37 -0.701326464010755 1102 38-39 -0.7732719545454003 1102 40-41 -0.7913410806391354 1102 42-43 -0.7473822807629418 1102 44-45 -0.8356117884160099 1102 46-47 -0.88372111733662 1102 48-49 -0.7854502363637295 1102 50-51 -0.7724778608388263 1102 52-53 -0.8188885047895411 1102 54-55 -0.8732720958647064 1102 56-57 -0.865686050356139 1102 58-59 -0.8190525868106633 1102 60-61 -0.9127756965548883 1102 62-63 -0.9634938238126765 1102 64-65 -0.9956346919709205 1102 66-67 -1.0494586713826735 1102 68-69 -1.0065137469628027 1102 70-71 -0.9535768920369492 1102 72-73 -1.0923554706737306 1102 74-75 -1.0062804568359631 1102 76-77 -0.7926337372479288 1102 78-79 -0.8094002914392853 1102 80-81 -0.7326963949558731 1102 82-83 -0.7614008401611763 1102 84-85 -0.9193419850067457 1102 86-87 -0.9570990209448951 1102 88-89 -0.8934286754146008 1102 90-91 -0.9682657326861097 1102 92-93 -1.0604992691377113 1102 94-95 -0.9706690844653352 1102 96-97 -1.0542160622845138 1102 98-99 -1.1579654376061157 1102 100 -1.0616608657466209 1103 1 -0.35621343035244024 1103 2 -0.28794369773790507 1103 3 -0.3945843598960508 1103 4 -0.2641485027199977 1103 5 -0.1904983130996527 1103 6 -0.3409507430951564 1103 7 -0.3619786185852192 1103 8 -0.36991317877100727 1103 9 -0.47371376325439485 1103 10-11 -0.4709610001137463 1103 12-13 -0.527983754366609 1103 14-15 -0.7482977963821682 1103 16-17 -0.8080613013270153 1103 18-19 -0.7766719084195799 1103 20-21 -0.7967875333381684 1103 22-23 -0.8722011973596295 1103 24-25 -0.7367080152996266 1103 26-27 -0.8471983049065699 1103 28-29 -0.8562948462004698 1103 30-31 -0.7522644714015101 1103 32-33 -0.7439935919600558 1103 34-35 -0.7512831465778049 1103 36-37 -0.7534536041862268 1103 38-39 -0.7527448590644283 1103 40-41 -0.9301152218092525 1103 42-43 -0.8722018650391732 1103 44-45 -0.7094168084855568 1103 46-47 -0.8276404504747958 1103 48-49 -0.8047729833818593 1103 50-51 -0.8014475057345507 1103 52-53 -0.875415767453525 1103 54-55 -0.8754037394362086 1103 56-57 -0.9849212309389443 1103 58-59 -1.0113311537681682 1103 60-61 -0.977071347478109 1103 62-63 -1.0251298949531886 1103 64-65 -1.040831910983627 1103 66-67 -0.9478703268505555 1103 68-69 -0.9531418589737797 1103 70-71 -0.9798564289159302 1103 72-73 -0.9948878839900992 1103 74-75 -0.9728362588243478 1103 76-77 -0.905808413326632 1103 78-79 -0.7501922260917198 1103 80-81 -0.6954281504176905 1103 82-83 -0.7598006839952944 1103 84-85 -0.7806512788484845 1103 86-87 -0.8029779657591156 1103 88-89 -0.7882735834387837 1103 90-91 -0.92771913380464 1103 92-93 -0.830413578936831 1103 94-95 -0.829612464245642 1103 96-97 -1.0438611525439576 1103 98-99 -1.0505484755462788 1103 100 -1.153955951175238 1104 1 -0.3185082941347517 1104 2 -0.3059685487399584 1104 3 -0.304284365055004 1104 4 -0.15334383469878077 1104 5 -0.1921190950733731 1104 6 -0.2836519765618064 1104 7 -0.29487149424751635 1104 8 -0.26595910790921806 1104 9 -0.41559255278118457 1104 10-11 -0.41640411028495805 1104 12-13 -0.5188698750777618 1104 14-15 -0.5988711779555018 1104 16-17 -0.5395997434065336 1104 18-19 -0.6055495857941438 1104 20-21 -0.561376144546621 1104 22-23 -0.7203153512662652 1104 24-25 -0.7273915686394474 1104 26-27 -0.8297958091045103 1104 28-29 -0.7787355516860544 1104 30-31 -0.6849419126791432 1104 32-33 -0.6113885704616919 1104 34-35 -0.6419901278450055 1104 36-37 -0.6566641876704082 1104 38-39 -0.7804722400274784 1104 40-41 -0.8181168497355173 1104 42-43 -0.6364614865215401 1104 44-45 -0.736165871180134 1104 46-47 -0.6951815710315117 1104 48-49 -0.6935443964795454 1104 50-51 -0.7211612184545046 1104 52-53 -0.706334172582487 1104 54-55 -0.6149214186517753 1104 56-57 -0.6497682054569864 1104 58-59 -0.7484604164158881 1104 60-61 -0.7500633212563272 1104 62-63 -0.8438345883949623 1104 64-65 -0.8068474363048992 1104 66-67 -0.7644100086103194 1104 68-69 -0.7766551339042351 1104 70-71 -0.7202149352547025 1104 72-73 -0.8094050146262006 1104 74-75 -0.6871958894411918 1104 76-77 -0.6721785894997367 1104 78-79 -0.6386760085883125 1104 80-81 -0.7022693069072794 1104 82-83 -0.6440716556657264 1104 84-85 -0.6807827823977526 1104 86-87 -0.5758838192441509 1104 88-89 -0.6608469212229657 1104 90-91 -0.6848249510287552 1104 92-93 -0.8487901161530864 1104 94-95 -0.8743949924016228 1104 96-97 -0.9808631860666743 1104 98-99 -0.8893157466563899 1104 100 -0.9830685760821645 1105 1 -0.35355877220167287 1105 2 -0.3113161676078988 1105 3 -0.35160735872227633 1105 4 -0.19041675701804905 1105 5 -0.19385494758488164 1105 6 -0.2700722610561499 1105 7 -0.31777549779981484 1105 8 -0.3280303435644569 1105 9 -0.4071231469846879 1105 10-11 -0.4073131088092481 1105 12-13 -0.4481198473430794 1105 14-15 -0.6222317099420351 1105 16-17 -0.5912957487745061 1105 18-19 -0.5323536011742647 1105 20-21 -0.6599699842756266 1105 22-23 -0.6383022540025394 1105 24-25 -0.6385524103323164 1105 26-27 -0.5885355398611765 1105 28-29 -0.6545246493344479 1105 30-31 -0.5889045569053835 1105 32-33 -0.5442710806920061 1105 34-35 -0.5769103728662444 1105 36-37 -0.5739719434926442 1105 38-39 -0.5417209717361189 1105 40-41 -0.5537765946027662 1105 42-43 -0.5432716289380863 1105 44-45 -0.6503522752660658 1105 46-47 -0.7502421888426696 1105 48-49 -0.6350224006398903 1105 50-51 -0.6417030760921563 1105 52-53 -0.7115969877710668 1105 54-55 -0.7327356888550653 1105 56-57 -0.7032197646073328 1105 58-59 -0.7228924128982257 1105 60-61 -0.7225238615515082 1105 62-63 -0.6257436605961288 1105 64-65 -0.609874600675937 1105 66-67 -0.6054243283643714 1105 68-69 -0.6680615171818616 1105 70-71 -0.710569240087068 1105 72-73 -0.7279176884125818 1105 74-75 -0.7484982227209187 1105 76-77 -0.6883587198541683 1105 78-79 -0.5309841454978184 1105 80-81 -0.50450118684277 1105 82-83 -0.5753911788041712 1105 84-85 -0.48956532901484096 1105 86-87 -0.5813479637780397 1105 88-89 -0.6076985028809787 1105 90-91 -0.59608445198133 1105 92-93 -0.637430130485729 1105 94-95 -0.5482297733804167 1105 96-97 -0.6288500055283173 1105 98-99 -0.6512280877394687 1105 100 -0.6985933986484021 1106 1 -0.3505365128968734 1106 2 -0.35836501815422395 1106 3 -0.3934086856288346 1106 4 -0.23914476890679026 1106 5 -0.28806460183022153 1106 6 -0.3632960206283613 1106 7 -0.34822294878898674 1106 8 -0.35124919178969805 1106 9 -0.46740669094523213 1106 10-11 -0.4954091149026212 1106 12-13 -0.5696051278485967 1106 14-15 -0.6764732503304103 1106 16-17 -0.7180899900729045 1106 18-19 -0.691767968590689 1106 20-21 -0.7340826314880147 1106 22-23 -0.6824256968431754 1106 24-25 -0.7093207148427183 1106 26-27 -0.7724028231938718 1106 28-29 -0.7919924036090293 1106 30-31 -0.7184335841985714 1106 32-33 -0.7168801161746927 1106 34-35 -0.711471967401792 1106 36-37 -0.6864936532504373 1106 38-39 -0.702820131006682 1106 40-41 -0.7866347452227842 1106 42-43 -0.7375126373167333 1106 44-45 -0.794980850912907 1106 46-47 -0.7479811928497782 1106 48-49 -0.7629309281393972 1106 50-51 -0.7996525135077377 1106 52-53 -0.8252012285501422 1106 54-55 -0.8015318981362753 1106 56-57 -0.8294412231242134 1106 58-59 -0.8289426934359199 1106 60-61 -0.9141039376696867 1106 62-63 -0.9131143752102489 1106 64-65 -0.8705326495706203 1106 66-67 -0.8171319544925879 1106 68-69 -0.8474698543995345 1106 70-71 -0.7665827036488935 1106 72-73 -0.7802712966937335 1106 74-75 -0.7773319033408939 1106 76-77 -0.8173077364654695 1106 78-79 -0.6593182694441211 1106 80-81 -0.6986642857060374 1106 82-83 -0.7151594332857165 1106 84-85 -0.626588329507971 1106 86-87 -0.7133664136394735 1106 88-89 -0.8171828472487839 1106 90-91 -0.8331207028698628 1106 92-93 -0.8582012944350836 1106 94-95 -0.8004641802163128 1106 96-97 -0.8250352511314674 1106 98-99 -0.931715847301529 1106 100 -0.9746319210462353 1107 1 -0.37800217656633706 1107 2 -0.3568641942814459 1107 3 -0.460100815756725 1107 4 -0.2800136019414339 1107 5 -0.2747592987532457 1107 6 -0.3027272572359294 1107 7 -0.38200393314913583 1107 8 -0.37194672258968353 1107 9 -0.5448199029074772 1107 10-11 -0.5412294086588503 1107 12-13 -0.5688476476679085 1107 14-15 -0.7388728334729251 1107 16-17 -0.7235665902278399 1107 18-19 -0.7299990350980678 1107 20-21 -0.6975605908796609 1107 22-23 -0.7330974734503783 1107 24-25 -0.7975510078441133 1107 26-27 -0.8050058779734783 1107 28-29 -0.8220369206563518 1107 30-31 -0.7935802013679663 1107 32-33 -0.8222743290519006 1107 34-35 -0.8113794445284626 1107 36-37 -0.8187950273615812 1107 38-39 -0.8028215290965122 1107 40-41 -0.7639939028978873 1107 42-43 -0.757153598957153 1107 44-45 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0.878125599107733 2311 28-29 0.8386631355388943 2311 30-31 0.8714744208038283 2311 32-33 1.0205980972879516 2311 34-35 1.0009579835950504 2311 36-37 1.044497076811055 2311 38-39 1.0657464052421162 2311 40-41 1.0569345708472255 2311 42-43 1.1363196331116754 2311 44-45 1.057237544299511 2311 46-47 1.1084666149212694 2311 48-49 1.0929015905864858 2311 50-51 1.1226567156553173 2311 52-53 1.1569779640644882 2311 54-55 1.1533061155237903 2311 56-57 1.1213986441533876 2311 58-59 1.1659292927716152 2311 60-61 1.2052322744811477 2311 62-63 1.2057763981751606 2311 64-65 1.2016184554599292 2311 66-67 1.263631835734195 2311 68-69 1.2366794537488843 2311 70-71 1.2586874195160505 2311 72-73 1.2878373307465836 2311 74-75 1.179325700847592 2311 76-77 1.101139730181302 2311 78-79 1.0044076477295931 2311 80-81 1.0628834778156744 2311 82-83 1.122180565045678 2311 84-85 1.101047768545616 2311 86-87 1.217318228207791 2311 88-89 1.2511032295984243 2311 90-91 1.2216052565884539 2311 92-93 1.278777919763204 2311 94-95 1.2913890589623485 2311 96-97 1.3705617701103527 2311 98-99 1.4521477603611253 2311 100 1.4497902035971038 2312 1 0.08625436161606359 2312 2 0.09238390249625894 2312 3 0.28963593784543207 2312 4 0.2299661092251597 2312 5 0.3135400862531128 2312 6 0.40406402428650523 2312 7 0.4343204649310479 2312 8 0.2471283384423586 2312 9 0.3556767666188776 2312 10-11 0.2419900520594922 2312 12-13 0.46434147490869293 2312 14-15 0.8356421745930547 2312 16-17 0.8573638800430388 2312 18-19 0.8209975755397494 2312 20-21 0.8338194702714432 2312 22-23 0.4627819275556462 2312 24-25 0.4938318622220379 2312 26-27 0.7625897186374289 2312 28-29 0.8960695849210367 2312 30-31 0.8810815578495195 2312 32-33 0.6988407136821593 2312 34-35 0.8693601689754118 2312 36-37 0.9176974379454848 2312 38-39 0.8979901440192819 2312 40-41 0.9357548268510882 2312 42-43 0.7927532341649055 2312 44-45 0.757344313248467 2312 46-47 0.9379424283497642 2312 48-49 0.9768729892590073 2312 50-51 0.9883210492296186 2312 52-53 0.991477418933222 2312 54-55 0.9866749569174473 2312 56-57 1.0022562216383477 2312 58-59 1.0918471141039063 2312 60-61 1.113477437893998 2312 62-63 1.114849628926173 2312 64-65 1.1973787086055836 2312 66-67 1.173935369245207 2312 68-69 1.2045084282459442 2312 70-71 1.0548768740744592 2312 72-73 1.0965200878225545 2312 74-75 1.1384288605706487 2312 76-77 1.050046785213965 2312 78-79 0.9413089752481909 2312 80-81 0.8888890177028941 2312 82-83 0.8805670970111201 2312 84-85 0.7367431355539118 2312 86-87 0.9280924520241136 2312 88-89 0.6233015806579267 2312 90-91 0.9487698903542565 2312 92-93 1.1336144754267714 2312 94-95 1.1596434459779523 2312 96-97 1.2372861137570759 2312 98-99 1.303269195751703 2312 100 1.4549672759289969 2313 1 -0.4321295491550714 2313 2 -0.08811821125072328 2313 3 0.21323403714756495 2313 4 0.053757642232802993 2313 5 0.19816134696237242 2313 6 0.313112518761109 2313 7 0.10284934344986851 2313 8 0.09292619536024205 2313 9 0.26433415387125336 2313 10-11 0.35874666016695755 2313 12-13 0.5511264093121326 2313 14-15 0.5725293644168588 2313 16-17 0.7283877338409397 2313 18-19 0.6328884710088261 2313 20-21 0.8149709398729712 2313 22-23 0.8381604417286113 2313 24-25 0.6418051835870813 2313 26-27 0.883400443717484 2313 28-29 0.8225365184016198 2313 30-31 0.7539831969387549 2313 32-33 0.9158006930763349 2313 34-35 0.9243643737341642 2313 36-37 0.8035372603359505 2313 38-39 0.5696655252482756 2313 40-41 0.7094475440480537 2313 42-43 0.7243542773707361 2313 44-45 0.8685957303941976 2313 46-47 0.8530638379132682 2313 48-49 0.9115606178166828 2313 50-51 0.8210070805124374 2313 52-53 0.8287434797388684 2313 54-55 0.9054710021958385 2313 56-57 0.8464150200523832 2313 58-59 0.8129468725324216 2313 60-61 0.8694687408066883 2313 62-63 0.9674195298807788 2313 64-65 1.0423058959927545 2313 66-67 1.0563618363811784 2313 68-69 1.0620542280523964 2313 70-71 0.9694841576511664 2313 72-73 1.0433696134315298 2313 74-75 0.7098092174135999 2313 76-77 0.8927692637908393 2313 78-79 0.7125206208420991 2313 80-81 0.8244199301859609 2313 82-83 0.8672583142701349 2313 84-85 0.8872915137968 2313 86-87 0.9765761176207626 2313 88-89 0.9733064866054804 2313 90-91 1.0804585870483017 2313 92-93 0.8356125018197886 2313 94-95 0.8620505387408066 2313 96-97 1.1517108801645648 2313 98-99 1.2079592034076612 2313 100 1.360030431659581 2314 1 -0.33471895896636994 2314 2 -0.16782074440696348 2314 3 0.1434835667550658 2314 4 -0.15237160632260327 2314 5 0.18191064067567453 2314 6 0.2474763327261016 2314 7 0.09621085832053211 2314 8 0.1608476251084099 2314 9 0.27714838877308523 2314 10-11 0.45072328118946103 2314 12-13 0.4893673395012783 2314 14-15 0.5478972853749795 2314 16-17 0.745330821999886 2314 18-19 0.8057993002901114 2314 20-21 0.4612785112176141 2314 22-23 0.6631687670077113 2314 24-25 0.8471956466308939 2314 26-27 0.8313339205226313 2314 28-29 0.7691523432677769 2314 30-31 0.741357368402042 2314 32-33 0.8449998389024316 2314 34-35 0.7854676695905951 2314 36-37 0.7602448874300123 2314 38-39 0.7897324267195316 2314 40-41 0.8274042806774276 2314 42-43 0.6397417946508028 2314 44-45 0.7186533608953525 2314 46-47 0.8903308798531029 2314 48-49 0.7323179143688492 2314 50-51 0.8947015485426917 2314 52-53 1.0255641772148394 2314 54-55 1.058481907213718 2314 56-57 0.8556554564439196 2314 58-59 0.8556978244711004 2314 60-61 0.9467707665369645 2314 62-63 0.6304649102006152 2314 64-65 0.7817359357031037 2314 66-67 0.936424163082421 2314 68-69 0.9780278758809686 2314 70-71 0.9797931516793454 2314 72-73 1.0017238785466915 2314 74-75 0.9949822951279401 2314 76-77 0.856044320441498 2314 78-79 0.8627872601653053 2314 80-81 0.8477283942043528 2314 82-83 0.8356227551179352 2314 84-85 0.8096387096210726 2314 86-87 0.9176151167896727 2314 88-89 1.014101939851134 2314 90-91 1.0994420394346172 2314 92-93 1.0910788207193676 2314 94-95 1.0919540901858795 2314 96-97 1.1786079690897147 2314 98-99 1.172928833147278 2314 100 1.2105388849010552 2315 1 -0.11701974074313881 2315 2 0.044471379515726994 2315 3 0.08207856516376211 2315 4 -0.09310120332052918 2315 5 0.2151796183306729 2315 6 0.3367923297920896 2315 7 0.4247127276514533 2315 8 0.35323673430976754 2315 9 0.5584834566529082 2315 10-11 0.5345230579328728 2315 12-13 0.6301182193196837 2315 14-15 0.743593550108109 2315 16-17 0.7544700519526799 2315 18-19 0.790360423103273 2315 20-21 0.8152518724573454 2315 22-23 0.8811576669381438 2315 24-25 0.8261878797201803 2315 26-27 0.8638785451260063 2315 28-29 0.8934621123867146 2315 30-31 0.9016272118424453 2315 32-33 0.8824580831177542 2315 34-35 0.8806375784552145 2315 36-37 0.9235086765944232 2315 38-39 0.9565862428908716 2315 40-41 0.8979917271001057 2315 42-43 0.976256182635467 2315 44-45 0.8572387357090179 2315 46-47 0.8765048441534162 2315 48-49 0.9432598742846494 2315 50-51 0.861428073564646 2315 52-53 0.9020279949998198 2315 54-55 0.8818020691389847 2315 56-57 0.9754383419182702 2315 58-59 0.9802486095278695 2315 60-61 1.006086494439863 2315 62-63 1.090210068206794 2315 64-65 1.0902503082683808 2315 66-67 1.11286342480728 2315 68-69 1.0695380390871279 2315 70-71 0.7395023243382752 2315 72-73 0.9570022614905653 2315 74-75 1.1109257651974715 2315 76-77 0.995639564072647 2315 78-79 0.9095056683645311 2315 80-81 0.9346479470365381 2315 82-83 0.9101179018638952 2315 84-85 0.8238509402567864 2315 86-87 0.8330799837696752 2315 88-89 0.9475011234618869 2315 90-91 1.077745133849863 2315 92-93 1.0941675010316594 2315 94-95 1.1442401707884606 2315 96-97 1.1726866042083017 2315 98-99 1.081030405560199 2315 100 1.0443241958189198 2316 1 0.46800420446037805 2316 2 0.4614764380593428 2316 3 0.46610188266095776 2316 4 0.30229227224929645 2316 5 0.387284103827362 2316 6 0.44381870261509704 2316 7 0.4748884671033551 2316 8 0.47872051167314567 2316 9 0.566732679346309 2316 10-11 0.49735961278548757 2316 12-13 0.6133859278200973 2316 14-15 0.7939554493553587 2316 16-17 0.773662787357587 2316 18-19 0.8064767041932512 2316 20-21 0.85850147847286 2316 22-23 0.8603007575505259 2316 24-25 0.9270868878186107 2316 26-27 0.9601278494552474 2316 28-29 0.924016050779386 2316 30-31 0.8007158733313418 2316 32-33 0.7237720105837937 2316 34-35 0.6644103236511967 2316 36-37 0.6559205301700857 2316 38-39 0.637374603033642 2316 40-41 0.7195415795145479 2316 42-43 0.8092136030058583 2316 44-45 0.7848570612271999 2316 46-47 0.8423362044026845 2316 48-49 0.5973624347978586 2316 50-51 0.6254530970414933 2316 52-53 0.7901709982199847 2316 54-55 0.885962553155764 2316 56-57 0.8952855573120715 2316 58-59 0.8580660663047084 2316 60-61 0.9795765716302114 2316 62-63 0.9646805930911952 2316 64-65 0.9968924301093409 2316 66-67 1.011071603252752 2316 68-69 0.9991154022548798 2316 70-71 0.8261001927533442 2316 72-73 0.9061822203638954 2316 74-75 0.8851074902307161 2316 76-77 0.7858356366039985 2316 78-79 0.8056867858387591 2316 80-81 0.7363250401518897 2316 82-83 0.6722319599472648 2316 84-85 0.7289697753448117 2316 86-87 0.7429542501918895 2316 88-89 0.7170629077909965 2316 90-91 0.7416499668744869 2316 92-93 0.76291138979132 2316 94-95 0.6959468241875584 2316 96-97 0.8028056028296469 2316 98-99 0.8619652130657762 2316 100 0.5952789426156961 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 7.0 12 37.0 13 218.0 14 990.0 15 2917.0 16 6893.0 17 13833.0 18 21734.0 19 30105.0 20 39551.0 21 50990.0 22 60765.0 23 62398.0 24 64053.0 25 69824.0 26 80680.0 27 97275.0 28 118229.0 29 143276.0 30 175007.0 31 215950.0 32 270216.0 33 351068.0 34 487488.0 35 667289.0 36 1006927.0 37 1540598.0 38 1364625.0 39 201172.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54516829083022 19.25031538425342 12.884959252130384 24.31955707278598 2 16.215014323631486 20.977618293487613 39.3570364962155 23.4503308866654 3 18.501672280126726 26.35453010566145 30.893986090414266 24.24981152379755 4 12.72232076235061 17.091655385494114 37.84028659544712 32.345737256708155 5 12.759157973066932 37.58990416521495 34.43396193400848 15.216975927709639 6 29.957698179640175 36.91732115439555 18.790681085763055 14.33429958020122 7 26.4853988417056 33.23651251902787 22.606435357724003 17.671653281542525 8 25.927520611413613 34.95344410072647 20.729007152755422 18.39002813510449 9 27.30309479456595 15.550652603948825 20.391570767956757 36.754681833528466 10-11 24.90745661286199 25.786512933818013 28.88873818138833 20.417292271931668 12-13 25.36204817072074 23.93944299441575 28.273354916143177 22.425153918720333 14-15 22.961874942566514 25.454325566564513 26.322632408814865 25.261167082054108 16-17 22.159327554371025 27.821541358806172 27.227438352723492 22.791692734099307 18-19 21.758489267704515 27.208565959952065 29.190526554778195 21.842418217565225 20-21 22.668798204737676 26.793985053758067 28.761941435333615 21.77527530617064 22-23 22.506865068955832 26.531038454184397 28.6367368603462 22.32535961651357 24-25 22.08384838300183 26.364241819126267 28.988871007860578 22.563038790011326 26-27 22.074900164631515 26.476754748457004 28.833701595707197 22.61464349120429 28-29 21.86897664776521 27.394069487001104 28.651923903321215 22.08502996191247 30-31 22.88608071918608 26.595377764738227 28.828346730913836 21.69019478516186 32-33 22.58206836190023 26.709836723891456 28.53945786208611 22.168637052122207 34-35 22.665435908176978 26.841822006057004 28.783409793714483 21.709332292051542 36-37 22.42250407191636 26.627907180259612 28.672872058340154 22.276716689483866 38-39 22.30753017300428 26.70000333908071 28.79271791999213 22.199748567922885 40-41 22.368206117218765 26.10683541688567 29.217312565324615 22.307645900570943 42-43 22.074669060464796 26.537711664019813 28.56814510436811 22.81947417114728 44-45 21.47114695440852 26.827641864423864 29.03258094139518 22.668630239772433 46-47 21.44614126601286 26.25507192123785 28.736170359015762 23.56261645373353 48-49 21.66123360150453 26.42442020183356 28.758989929319412 23.1553562673425 50-51 21.511980077836874 26.607494105398814 28.66860617232604 23.21191964443827 52-53 21.315501927407404 26.85663546835615 28.300360562031578 23.52750204220487 54-55 21.00518573974765 26.53568254828629 28.716257834465097 23.742873877500966 56-57 21.5031326929345 26.381873515479953 28.148435312282793 23.966558479302755 58-59 21.016400217034523 26.545307076411717 28.440635514709466 23.997657191844297 60-61 21.571311030695846 26.600224691168624 28.024561213925814 23.803903064209713 62-63 21.43813980520065 26.50040194180655 27.868102897662382 24.193355355330414 64-65 21.014095778154633 26.212060099314314 27.864722642188045 24.90912148034301 66-67 21.138781892483152 26.226623195302135 27.565220350356938 25.06937456185777 68-69 20.866507050484973 26.348350237144942 27.673261180432878 25.111881531937208 70-71 20.913344994139255 26.122833379780495 27.654623546892264 25.309198079187983 72-73 20.910364876214025 26.34713144008601 27.280587487567843 25.461916196132123 74-75 21.064666972599415 26.385488566551054 27.084499395660306 25.465345065189226 76-77 20.745234020976028 26.288053291847902 27.26681228665693 25.699900400519148 78-79 20.670076274142296 26.341349133058756 27.36848714376579 25.620087449033154 80-81 20.653659655041658 26.37113446219782 27.161217823141765 25.813988059618758 82-83 20.882772278170382 26.04460765528735 27.349214747725043 25.72340531881723 84-85 20.972473838148268 26.279520812022373 26.90735063781822 25.840654712011148 86-87 20.573272205883196 26.416004349147965 27.14273890729898 25.86798453766986 88-89 20.597430037665536 25.850345709752272 27.203478624287094 26.348745628295106 90-91 21.03528967972866 25.46789172213811 27.173558875572816 26.323259722560415 92-93 20.876979390751195 25.526120638798293 27.207970536474164 26.388929433976344 94-95 20.30762763445454 25.35231516192667 27.597295426790176 26.742761776828615 96-97 20.3233338948859 25.217554959070238 27.68288737030953 26.776223775734337 98-99 20.31977595620153 25.38908471113605 27.459701965633478 26.831437367028947 100 20.258710905906774 25.405040797032086 27.54728223487739 26.788966062183743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2366.0 1 1702.0 2 2249.0 3 3337.0 4 2382.5 5 1671.0 6 2323.0 7 3819.0 8 5661.0 9 6707.0 10 5891.5 11 4872.5 12 5040.5 13 5636.5 14 6311.0 15 6986.5 16 7783.5 17 8647.5 18 9481.0 19 10469.0 20 11666.5 21 13112.0 22 15037.0 23 17634.0 24 21101.5 25 25546.0 26 31362.5 27 38186.5 28 46524.0 29 57560.0 30 69948.5 31 81811.5 32 96109.0 33 109624.0 34 121515.5 35 134746.5 36 149222.5 37 167953.5 38 181207.5 39 186374.5 40 190650.5 41 196662.0 42 206867.5 43 219053.5 44 232688.5 45 252779.5 46 272114.0 47 289575.5 48 308845.5 49 321976.0 50 329108.5 51 332328.5 52 324980.5 53 305862.0 54 283875.0 55 254709.5 56 222526.5 57 191315.0 58 160649.0 59 134637.0 60 106722.5 61 81231.0 62 63300.5 63 48530.5 64 35192.5 65 24784.0 66 17981.5 67 13761.0 68 10514.5 69 7971.0 70 6329.5 71 4937.5 72 3809.5 73 2880.0 74 2136.5 75 1516.5 76 1024.5 77 671.0 78 440.5 79 284.5 80 167.0 81 111.5 82 79.0 83 53.0 84 32.5 85 21.5 86 21.0 87 11.0 88 5.5 89 6.0 90 3.5 91 2.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05097885847109897 2 0.0 3 0.0 4 0.013549570291055408 5 0.003933294681597696 6 1.399748997009856E-5 7 1.819673696112813E-4 8 5.319046188637453E-4 9 0.0019876435757539956 10-11 7.908581833105688E-4 12-13 3.709334842076119E-4 14-15 6.298870486544352E-5 16-17 9.098368480564065E-5 18-19 0.0 20-21 1.399748997009856E-5 22-23 0.0 24-25 6.998744985049281E-5 26-27 2.0996234955147843E-5 28-29 9.518293179667022E-4 30-31 8.678443781461109E-4 32-33 0.013892508795322823 34-35 0.023221835860393513 36-37 0.031221401378304845 38-39 0.02034535167153826 40-41 1.2597740973088704E-4 42-43 0.0017006950313669753 44-45 0.009770247999128796 46-47 0.014158461104754695 48-49 0.0053260449336225025 50-51 0.01031615010796264 52-53 0.015719181236420684 54-55 0.009826237959009191 56-57 0.002862486698885156 58-59 0.008034559242836574 60-61 0.0015397238967108419 62-63 9.658268079368008E-4 64-65 0.019050583849304144 66-67 0.04467998798455461 68-69 0.05437324978884786 70-71 0.0477034458180959 72-73 0.040319769858868905 74-75 0.028631865733836605 76-77 0.028449898364225325 78-79 0.012478762308342866 80-81 0.0020296360456642914 82-83 0.02674920333285835 84-85 0.04688459265484513 86-87 0.047997393107467964 88-89 0.05863548548474287 90-91 0.03464378767599394 92-93 0.026364272358680642 94-95 0.007474659644032632 96-97 0.0035483637074199856 98-99 0.02247996889197829 100 0.018742639069961976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7144138.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.61413347024442 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96898198944395 48.96315093345326 2 14.494304757464658 18.440852747995105 3 4.207677493000552 8.030032729184414 4 1.6668603252515495 4.241435008272283 5 0.7999283664261069 2.544337493423247 6 0.4598890696157666 1.7553266793626343 7 0.31408675024381083 1.3986249515871574 8 0.20880975625664033 1.0626601363519297 9 0.1419305932278679 0.8125912530997701 >10 0.695188142597545 7.596513892732607 >50 0.027427763683268682 1.1942445873285925 >100 0.012903243097568039 1.5551375947535468 >500 0.0011968835518539852 0.5098663581687483 >1k 7.130078719015217E-4 0.873809884572563 >5k 2.5464566776136077E-5 0.0839597461028297 >10k+ 7.639370032840823E-5 0.9374560036112015 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24608 0.34445023318418544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20691 0.2896220649713093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12572 0.17597644390407913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.598995988039425E-5 2 0.0 0.0 0.0 0.0 5.598995988039425E-5 3 0.0 0.0 0.0 4.199246991029569E-5 5.598995988039425E-5 4 0.0 0.0 0.0 4.199246991029569E-5 5.598995988039425E-5 5 0.0 1.3997489970098562E-5 0.0 5.598995988039425E-5 5.598995988039425E-5 6 0.0 1.3997489970098562E-5 0.0 5.598995988039425E-5 1.2597740973088707E-4 7 0.0 1.3997489970098562E-5 0.0 6.998744985049281E-5 1.2597740973088707E-4 8 0.0 1.3997489970098562E-5 0.0 8.398493982059137E-5 1.2597740973088707E-4 9 0.0 1.3997489970098562E-5 0.0 8.398493982059137E-5 1.3997489970098563E-4 10-11 0.0 1.3997489970098562E-5 0.0 1.0498117477573921E-4 1.3997489970098563E-4 12-13 0.0 1.3997489970098562E-5 0.0 1.6796987964118275E-4 2.799497994019712E-4 14-15 0.0 1.3997489970098562E-5 0.0 1.7496862462623204E-4 3.2894101429731617E-4 16-17 0.0 1.3997489970098562E-5 0.0 2.589535644468234E-4 3.7793222919266115E-4 18-19 0.0 1.3997489970098562E-5 0.0 3.849309741777104E-4 3.849309741777104E-4 20-21 0.0 1.3997489970098562E-5 0.0 7.768606933404702E-4 4.269234440880061E-4 22-23 6.998744985049281E-6 1.3997489970098562E-5 0.0 0.002764504269094466 4.409209340581047E-4 24-25 4.199246991029569E-5 1.3997489970098562E-5 0.0 0.008321507787223594 4.47919679043154E-4 26-27 5.598995988039425E-5 1.3997489970098562E-5 0.0 0.016279080835224627 4.47919679043154E-4 28-29 5.598995988039425E-5 1.3997489970098562E-5 0.0 0.043049280403038126 4.549184240282033E-4 30-31 5.598995988039425E-5 1.3997489970098562E-5 0.0 0.12953977092827715 4.6191716901325256E-4 32-33 8.398493982059137E-5 1.3997489970098562E-5 0.0 0.31879983281397983 4.689159139983018E-4 34-35 9.798242979068993E-5 1.3997489970098562E-5 0.0 0.5989595945655025 4.899121489534496E-4 36-37 1.119799197607885E-4 1.3997489970098562E-5 0.0 1.0255540976392112 4.899121489534496E-4 38-39 1.119799197607885E-4 1.3997489970098562E-5 0.0 1.6377063265015317 5.039096389235483E-4 40-41 1.119799197607885E-4 1.3997489970098562E-5 0.0 2.3011593561042636 5.109083839085975E-4 42-43 1.119799197607885E-4 1.3997489970098562E-5 0.0 2.9071596881247252 5.249058738786961E-4 44-45 1.119799197607885E-4 1.3997489970098562E-5 0.0 3.614613827448462 5.389033638487946E-4 46-47 1.119799197607885E-4 1.3997489970098562E-5 0.0 4.415088286368489 5.459021088338439E-4 48-49 1.119799197607885E-4 1.3997489970098562E-5 0.0 5.221910606989955 5.73897088774041E-4 50-51 1.4697364468603492E-4 1.3997489970098562E-5 0.0 6.025653479817999 5.73897088774041E-4 52-53 1.539723896710842E-4 1.3997489970098562E-5 0.0 6.792561957789729 6.438845386245338E-4 54-55 1.539723896710842E-4 1.3997489970098562E-5 0.0 7.636554613026792 6.858770085348295E-4 56-57 1.539723896710842E-4 1.3997489970098562E-5 0.0 8.543619958069119 6.998744985049282E-4 58-59 1.539723896710842E-4 1.3997489970098562E-5 0.0 9.52664968117917 7.138719884750267E-4 60-61 1.6796987964118275E-4 1.3997489970098562E-5 0.0 10.477926098292054 7.138719884750267E-4 62-63 1.6796987964118275E-4 1.3997489970098562E-5 0.0 11.44439679076748 7.418669684152238E-4 64-65 1.6796987964118275E-4 1.3997489970098562E-5 0.0 12.36175029093783 7.558644583853223E-4 66-67 1.6796987964118275E-4 2.7994979940197123E-5 0.0 13.346949624993247 7.558644583853223E-4 68-69 1.6796987964118275E-4 2.7994979940197123E-5 0.0 14.404466990979177 7.558644583853223E-4 70-71 1.6796987964118275E-4 3.499372492524641E-5 0.0 15.484744275656489 7.698619483554209E-4 72-73 1.6796987964118275E-4 4.199246991029569E-5 0.0 16.477803200330115 8.258519082358152E-4 74-75 1.6796987964118275E-4 4.199246991029569E-5 0.0 17.437947027339057 8.538468881760122E-4 76-77 1.6796987964118275E-4 5.598995988039425E-5 0.0 18.46865920003225 8.538468881760122E-4 78-79 1.6796987964118275E-4 5.598995988039425E-5 0.0 19.57394999928613 8.538468881760122E-4 80-81 1.6796987964118275E-4 5.598995988039425E-5 0.0 20.632293217180298 8.608456331610616E-4 82-83 1.6796987964118275E-4 5.598995988039425E-5 0.0 21.674504327883923 9.168355930414557E-4 84-85 1.6796987964118275E-4 5.598995988039425E-5 0.0 22.675086903416478 9.238343380265051E-4 86-87 1.6796987964118275E-4 5.598995988039425E-5 0.0 23.68964597268418 9.588280629517516E-4 88 1.6796987964118275E-4 5.598995988039425E-5 0.0 24.455462646438242 9.93821787876998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11675 0.0 51.986942 1 TACCTGG 6385 0.0 42.68688 2 GTATCAA 21070 0.0 40.36444 1 ACCTGGG 7710 0.0 34.498363 3 ATCAACG 25660 0.0 32.342266 3 CAACGCA 25990 0.0 31.981153 5 TATCAAC 26615 0.0 31.569544 2 TCAACGC 27020 0.0 30.871126 4 AACGCAG 27845 0.0 30.28955 6 GTACATG 22605 0.0 28.035631 1 TACATGG 22725 0.0 27.482002 2 CCTGGGG 7265 0.0 27.167715 4 ACGCAGA 30955 0.0 26.821396 7 CGCAGAG 31400 0.0 26.396389 8 ACATGGG 22685 0.0 25.853075 3 TGGGGGG 13790 0.0 24.839272 6 CATGGGG 17455 0.0 24.68817 4 ATGGGGG 13385 0.0 23.835482 5 GTACCTG 11650 0.0 23.769186 1 GCAGAGT 34175 0.0 23.730223 9 >>END_MODULE