FastQCFastQC Report
Mon 6 Jun 2016
ERR1211380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10238293
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300510.2935157257171679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257890.25188769260657023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140490.13722014011515396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149050.053.7694971
GTATCAA271100.040.3375241
ATCAACG316500.033.374243
CAACGCA316900.033.287615
TATCAAC335000.032.1879432
TACCTGG71500.031.0705452
AACGCAG344250.031.0665996
TCAACGC341350.031.0269494
GTACATG321000.028.6802651
TACATGG326350.027.661642
ACGCAGA381150.027.578777
CGCAGAG391250.026.8158868
ACATGGG327550.026.7659853
GTACTAG43100.026.6732671
GTGGTAT59850.025.5848521
CATGGGG251050.024.997634
ATGGGGG193600.023.893065
GCAGAGT429550.023.800689
ACCTGGG93800.022.8530433
CTGTGCG51900.022.1892459
Produced by FastQC (version 0.11.5)