FastQCFastQC Report
Mon 6 Jun 2016
ERR1211380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10238293
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288070.2813652627444829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256500.250530044412677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148520.14506324442951574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158250.053.24391
GTATCAA278350.038.6154061
CAACGCA328850.031.8070095
ATCAACG330400.031.577243
TACCTGG69000.030.7174022
TATCAAC348350.030.6243632
TCAACGC350950.029.835794
AACGCAG356600.029.738586
GTGGTAT61950.027.3235261
GTACATG325200.026.9796711
ACGCAGA398750.026.1944227
TACATGG330100.026.0390512
CGCAGAG407200.025.5237968
GTACTAG45050.025.4665781
ACATGGG327600.025.0329953
CATGGGG250800.023.8918784
ACCTGGG88100.023.1512833
GCAGAGT447300.022.96259
ATGGGGG193150.022.795045
TGGGGGG185700.022.6201616
Produced by FastQC (version 0.11.5)