##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211380_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10238293 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06431023218421 33.0 31.0 34.0 30.0 34.0 2 32.19848689620428 33.0 31.0 34.0 30.0 34.0 3 32.277647650833984 34.0 31.0 34.0 30.0 34.0 4 35.921380937232406 37.0 35.0 37.0 35.0 37.0 5 35.77533002815997 37.0 35.0 37.0 35.0 37.0 6 35.811776631123955 37.0 35.0 37.0 35.0 37.0 7 35.74143502242024 37.0 35.0 37.0 33.0 37.0 8 35.72815800446422 37.0 35.0 37.0 33.0 37.0 9 37.317070531191085 39.0 37.0 39.0 34.0 39.0 10-11 37.29498599034038 39.0 37.0 39.0 34.0 39.0 12-13 37.207217648488864 39.0 37.0 39.0 33.0 39.0 14-15 38.42024822887956 40.0 38.0 41.0 33.5 41.0 16-17 38.40793460394228 40.0 38.0 41.0 33.5 41.0 18-19 38.407625470378704 40.0 38.0 41.0 33.5 41.0 20-21 38.366578881850714 40.0 38.0 41.0 34.0 41.0 22-23 38.26543081937585 40.0 38.0 41.0 34.0 41.0 24-25 38.14577732831049 40.0 37.5 41.0 33.0 41.0 26-27 38.01625847199333 40.0 37.0 41.0 33.0 41.0 28-29 37.94272746443181 40.0 37.0 41.0 33.0 41.0 30-31 37.99529320952233 40.0 37.0 41.0 33.0 41.0 32-33 37.84378123384435 40.0 37.0 41.0 33.0 41.0 34-35 37.73258217947074 40.0 37.0 41.0 33.0 41.0 36-37 37.58817382936785 40.0 37.0 41.0 32.5 41.0 38-39 37.470490442107874 40.0 36.0 41.0 32.0 41.0 40-41 37.39981108178873 40.0 36.0 41.0 32.0 41.0 42-43 37.26113474189496 40.0 36.0 41.0 31.0 41.0 44-45 37.10262018287619 39.0 36.0 41.0 31.0 41.0 46-47 36.88046986934248 39.0 35.5 41.0 31.0 41.0 48-49 36.65502154509546 39.0 35.0 41.0 30.0 41.0 50-51 36.46935397336255 39.0 35.0 41.0 30.0 41.0 52-53 36.25664356353154 39.0 35.0 40.0 29.5 41.0 54-55 36.05090399346844 38.5 35.0 40.0 29.0 41.0 56-57 35.811051998609535 38.0 35.0 40.0 28.0 41.0 58-59 35.536102404961454 38.0 34.0 40.0 28.0 41.0 60-61 35.27748878646079 37.5 34.0 40.0 28.0 41.0 62-63 34.98811369238993 37.0 34.0 40.0 27.0 41.0 64-65 34.641073419172514 36.5 34.0 39.5 26.5 41.0 66-67 34.28746828206616 36.0 33.0 39.0 26.0 40.5 68-69 33.92091430671108 35.5 33.0 39.0 26.0 40.0 70-71 33.554237117456985 35.0 32.5 38.0 25.5 40.0 72-73 33.20467786964096 35.0 32.0 37.0 25.0 39.5 74-75 32.83878694426893 35.0 32.0 37.0 25.0 39.0 76-77 31.819036337405073 34.0 30.5 36.0 23.5 38.0 78-79 32.29202880792726 34.5 31.5 36.0 24.5 38.0 80-81 32.17534226652822 35.0 32.0 35.5 24.5 37.0 82-83 31.906910946971337 34.0 31.0 35.0 24.0 37.0 84-85 31.63087899516062 34.0 31.0 35.0 23.5 36.5 86-87 31.360780698501205 34.0 31.0 35.0 23.0 36.0 88-89 31.11227643123712 34.0 31.0 35.0 21.5 36.0 90-91 30.879317235793117 34.0 30.0 35.0 20.0 35.0 92-93 30.686639071571793 34.0 30.0 35.0 20.0 35.0 94-95 30.506499618637598 34.0 30.0 35.0 19.5 35.0 96-97 30.31420086336658 34.0 29.5 35.0 19.0 35.0 98-99 30.094179957537843 34.0 29.0 35.0 18.5 35.0 100 28.254661494840985 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2837670742807674 1101 2 -0.28213082119413 1101 3 -0.2906821220004936 1101 4 -0.16386670833842487 1101 5 -0.21526031004967905 1101 6 -0.20584577640612167 1101 7 -0.20453364983011824 1101 8 -0.30288890612886377 1101 9 -0.4353331819088666 1101 10-11 -0.39941512269508195 1101 12-13 -0.41073420071964506 1101 14-15 -0.5473443603142982 1101 16-17 -0.6074278999584379 1101 18-19 -0.6291936424783486 1101 20-21 -0.6249456259131847 1101 22-23 -0.7242318819369231 1101 24-25 -0.7390081759064984 1101 26-27 -0.7579272542964404 1101 28-29 -0.7163157947108303 1101 30-31 -0.6701147068257001 1101 32-33 -0.5513183015843239 1101 34-35 -0.5979303115019476 1101 36-37 -0.661053073467393 1101 38-39 -0.6988255542565227 1101 40-41 -0.6836417316677412 1101 42-43 -0.7145145959135775 1101 44-45 -0.7242950767778709 1101 46-47 -0.7220115847814768 1101 48-49 -0.6294190005823666 1101 50-51 -0.7489466159596816 1101 52-53 -0.8242121340717361 1101 54-55 -0.8401714788190233 1101 56-57 -0.8826769583594398 1101 58-59 -0.7872754114685705 1101 60-61 -0.8509724916105839 1101 62-63 -0.8738779434336763 1101 64-65 -0.9123337817410402 1101 66-67 -0.8861548043038354 1101 68-69 -0.9504840805243759 1101 70-71 -0.962223510093537 1101 72-73 -1.076823862474896 1101 74-75 -1.085192857804607 1101 76-77 -0.9088303952747729 1101 78-79 -0.8007122114527263 1101 80-81 -0.7795703967121881 1101 82-83 -0.8283883138942336 1101 84-85 -0.8679195753414106 1101 86-87 -1.0028392749007224 1101 88-89 -1.0570570842161118 1101 90-91 -1.1862484033367693 1101 92-93 -1.316635431121778 1101 94-95 -1.4127016507650474 1101 96-97 -1.5080411365448008 1101 98-99 -1.5703267863947517 1101 100 -1.4956474837574625 1102 1 -0.33738904166372663 1102 2 -0.2926534511620815 1102 3 -0.3797299181092981 1102 4 -0.24566457620169047 1102 5 -0.2075440687236778 1102 6 -0.2985305745124123 1102 7 -0.32374443221632987 1102 8 -0.4000533210947026 1102 9 -0.49562780507393 1102 10-11 -0.48795752809083837 1102 12-13 -0.44511940791001336 1102 14-15 -0.6050805350515702 1102 16-17 -0.7069219891801097 1102 18-19 -0.7185052898895066 1102 20-21 -0.7277072162044433 1102 22-23 -0.7547985916198954 1102 24-25 -0.7614802331261856 1102 26-27 -0.8282605739553262 1102 28-29 -0.8580122542082194 1102 30-31 -0.7343091649809566 1102 32-33 -0.7267640520836238 1102 34-35 -0.7911436923234234 1102 36-37 -0.7250786740627788 1102 38-39 -0.7403758972304857 1102 40-41 -0.8190562026042372 1102 42-43 -0.6815566224254255 1102 44-45 -0.7148682207230692 1102 46-47 -0.8364584964447133 1102 48-49 -0.8503978667294874 1102 50-51 -0.8956628873985508 1102 52-53 -0.9154486231030177 1102 54-55 -0.8483954747911682 1102 56-57 -0.8944325067286343 1102 58-59 -0.9812667263056198 1102 60-61 -0.9681686029188015 1102 62-63 -0.9807683792803843 1102 64-65 -0.9084386564329492 1102 66-67 -0.9326670610423875 1102 68-69 -1.0282691173951832 1102 70-71 -1.050814285197383 1102 72-73 -1.0387039682976393 1102 74-75 -1.0012917441084426 1102 76-77 -0.8879605573591185 1102 78-79 -0.8246001736798405 1102 80-81 -0.7098984327987523 1102 82-83 -0.7065954983015281 1102 84-85 -0.9037914418753488 1102 86-87 -0.9926826363292172 1102 88-89 -0.9996476959539962 1102 90-91 -0.9996241769066003 1102 92-93 -1.1592794241059146 1102 94-95 -1.2826611271327693 1102 96-97 -1.3590024099380997 1102 98-99 -1.4733967281197984 1102 100 -1.5358476936165353 1103 1 -0.36746606285381844 1103 2 -0.3238885911122189 1103 3 -0.38748682571233317 1103 4 -0.20812155133392451 1103 5 -0.21378884730741987 1103 6 -0.26907694987445296 1103 7 -0.3066386738483473 1103 8 -0.30489189888985635 1103 9 -0.4510603762093268 1103 10-11 -0.4580336437321719 1103 12-13 -0.516790862521141 1103 14-15 -0.6813651349977334 1103 16-17 -0.78018545928132 1103 18-19 -0.6901351594156253 1103 20-21 -0.6936963739938236 1103 22-23 -0.765505801701174 1103 24-25 -0.7539178340714585 1103 26-27 -0.8629416452122953 1103 28-29 -0.87677649766038 1103 30-31 -0.7072344863919966 1103 32-33 -0.7229067542736942 1103 34-35 -0.7698610179341401 1103 36-37 -0.7706045597565634 1103 38-39 -0.7847665560320465 1103 40-41 -0.8559612025461973 1103 42-43 -0.902731556763193 1103 44-45 -0.8589877356570454 1103 46-47 -0.852046194882945 1103 48-49 -0.9477375242939061 1103 50-51 -0.8766275579413545 1103 52-53 -0.9249791441649577 1103 54-55 -0.8642995547548864 1103 56-57 -0.929760806671851 1103 58-59 -1.082903672058869 1103 60-61 -0.9797154888816095 1103 62-63 -0.9988300991554624 1103 64-65 -0.9723894579394425 1103 66-67 -0.9708464292058565 1103 68-69 -0.9602949525858122 1103 70-71 -0.9539741079369364 1103 72-73 -0.9693681894040012 1103 74-75 -0.892267957866558 1103 76-77 -0.85483495778114 1103 78-79 -0.7799194608089941 1103 80-81 -0.7224625783981189 1103 82-83 -0.7514336696123323 1103 84-85 -0.7749280368550266 1103 86-87 -0.8910906967837136 1103 88-89 -0.9322615230544287 1103 90-91 -1.0777425492723758 1103 92-93 -1.0826622230407104 1103 94-95 -1.0741468241816428 1103 96-97 -1.2719105881247827 1103 98-99 -1.3244052233895935 1103 100 -1.3664795876444735 1104 1 -0.39647574355917925 1104 2 -0.3420171400765355 1104 3 -0.36425505285564697 1104 4 -0.17804270338184125 1104 5 -0.2392114018410183 1104 6 -0.3143785045910903 1104 7 -0.30040836492455725 1104 8 -0.3566333042047276 1104 9 -0.5367165444745723 1104 10-11 -0.5040803119842465 1104 12-13 -0.527174540138823 1104 14-15 -0.6129391900325487 1104 16-17 -0.6795510671854714 1104 18-19 -0.7046109526404507 1104 20-21 -0.7053122074808655 1104 22-23 -0.7054443707316551 1104 24-25 -0.7581085969718586 1104 26-27 -0.7665908750610484 1104 28-29 -0.7259045155881338 1104 30-31 -0.7642586924965826 1104 32-33 -0.7339943041523398 1104 34-35 -0.8113082870205872 1104 36-37 -0.804998423845511 1104 38-39 -0.9020306229419432 1104 40-41 -0.8937745806438713 1104 42-43 -0.7618030630272372 1104 44-45 -0.8809121407009215 1104 46-47 -0.8724140374041696 1104 48-49 -0.9398140819346636 1104 50-51 -0.9180601269928772 1104 52-53 -0.8544068352754763 1104 54-55 -0.8777219042997686 1104 56-57 -0.956330246283926 1104 58-59 -0.9336276761323887 1104 60-61 -0.9466194455239574 1104 62-63 -0.9742355465895756 1104 64-65 -0.9857465308586058 1104 66-67 -1.0062628192542178 1104 68-69 -0.8812815236362468 1104 70-71 -0.9516397670953296 1104 72-73 -0.9574558140357752 1104 74-75 -0.9198058703684211 1104 76-77 -0.900242730397256 1104 78-79 -0.8882744065466319 1104 80-81 -0.9092915165092421 1104 82-83 -0.9658135967269814 1104 84-85 -0.8650771569741131 1104 86-87 -0.8440636085546309 1104 88-89 -0.8977459998528339 1104 90-91 -0.8646198722043685 1104 92-93 -1.0490862138365493 1104 94-95 -1.12175372555334 1104 96-97 -1.2694131880721393 1104 98-99 -1.2166840556287006 1104 100 -1.29261789446727 1105 1 -0.39696886222849415 1105 2 -0.35034952740924297 1105 3 -0.35170518502610904 1105 4 -0.2485024891833305 1105 5 -0.2704830253297317 1105 6 -0.3486160762515027 1105 7 -0.3880659811882623 1105 8 -0.38222837086101435 1105 9 -0.5242404209081286 1105 10-11 -0.49264175867708104 1105 12-13 -0.5372908588086105 1105 14-15 -0.723508342263429 1105 16-17 -0.7231828629094395 1105 18-19 -0.7226821110609691 1105 20-21 -0.8245810856550193 1105 22-23 -0.7798369150391622 1105 24-25 -0.7817270754037153 1105 26-27 -0.8525836339274946 1105 28-29 -0.8095846082736173 1105 30-31 -0.785174789362074 1105 32-33 -0.794691351980525 1105 34-35 -0.7863549798139289 1105 36-37 -0.8558299526336768 1105 38-39 -0.8663669524585984 1105 40-41 -0.8183517598456049 1105 42-43 -0.8393603423856675 1105 44-45 -0.8000877600363552 1105 46-47 -0.9193012505775897 1105 48-49 -0.8201720001765267 1105 50-51 -0.8883725533206288 1105 52-53 -0.8859280139595285 1105 54-55 -0.9192747801117065 1105 56-57 -0.8612377218919747 1105 58-59 -0.9058882991432782 1105 60-61 -0.9229449905873892 1105 62-63 -0.851091672407982 1105 64-65 -0.8220309252405258 1105 66-67 -0.9252520499646621 1105 68-69 -0.9716772413437695 1105 70-71 -0.971808949801023 1105 72-73 -0.9868918967257656 1105 74-75 -0.9680017100581679 1105 76-77 -0.9703529453465727 1105 78-79 -0.8985424540377522 1105 80-81 -0.7733693977333189 1105 82-83 -0.8463225409151605 1105 84-85 -0.7200715885908977 1105 86-87 -0.765059501321808 1105 88-89 -0.8386970210795184 1105 90-91 -0.9084423083709865 1105 92-93 -0.9303606695658608 1105 94-95 -1.0506764813414229 1105 96-97 -1.0703975288005019 1105 98-99 -1.1194218482684057 1105 100 -1.1360107749103534 1106 1 -0.3676578335070815 1106 2 -0.35619495824984426 1106 3 -0.37800360278502154 1106 4 -0.20736634652205765 1106 5 -0.27347435832562184 1106 6 -0.3169062495606525 1106 7 -0.33045799672299125 1106 8 -0.36050224122636365 1106 9 -0.4520114463010714 1106 10-11 -0.4810931257300908 1106 12-13 -0.5387269447905112 1106 14-15 -0.6790173403907005 1106 16-17 -0.7340830260714526 1106 18-19 -0.6831099983749809 1106 20-21 -0.6916474532900239 1106 22-23 -0.7102402878377134 1106 24-25 -0.7789484557873791 1106 26-27 -0.8275965067658575 1106 28-29 -0.7957814499251512 1106 30-31 -0.8354174156296779 1106 32-33 -0.7606605145283609 1106 34-35 -0.706980784476805 1106 36-37 -0.767902224628223 1106 38-39 -0.8350283854083429 1106 40-41 -0.8294318798082614 1106 42-43 -0.7959794294862235 1106 44-45 -0.8059342846042128 1106 46-47 -0.86121328556343 1106 48-49 -0.914052300102604 1106 50-51 -0.9311470883628132 1106 52-53 -1.0126949800555707 1106 54-55 -0.9863013913133614 1106 56-57 -1.0761308998709609 1106 58-59 -1.0500986572309117 1106 60-61 -1.1357566840740176 1106 62-63 -1.1067310737578069 1106 64-65 -1.0770874362496983 1106 66-67 -0.9831736579585026 1106 68-69 -1.0055532666743972 1106 70-71 -0.9437645456234165 1106 72-73 -0.9769641786190917 1106 74-75 -1.0348434150671544 1106 76-77 -1.0538297337143732 1106 78-79 -0.9210635245276109 1106 80-81 -0.8463630865514347 1106 82-83 -0.8856692353090239 1106 84-85 -0.8277700055190209 1106 86-87 -0.8808224222620744 1106 88-89 -0.9818687028502282 1106 90-91 -0.8835191808254201 1106 92-93 -0.868645717160895 1106 94-95 -0.9511971154009657 1106 96-97 -0.9817532989652271 1106 98-99 -1.0995069088732663 1106 100 -1.1216412536553229 1107 1 -0.3525932012656696 1107 2 -0.3219558428525531 1107 3 -0.374050081654719 1107 4 -0.2342002344928389 1107 5 -0.24332446786966244 1107 6 -0.2679067350256261 1107 7 -0.3133477458658831 1107 8 -0.35351380937885324 1107 9 -0.4410015785336867 1107 10-11 -0.44056327718006827 1107 12-13 -0.5072802591580157 1107 14-15 -0.7017501183319865 1107 16-17 -0.6566868287965946 1107 18-19 -0.7081413146390574 1107 20-21 -0.711181255990013 1107 22-23 -0.7094412829151509 1107 24-25 -0.7655091250750559 1107 26-27 -0.6920870703831525 1107 28-29 -0.6989206853052323 1107 30-31 -0.7145676381134933 1107 32-33 -0.6991868139017896 1107 34-35 -0.7515355904543171 1107 36-37 -0.7525579469523151 1107 38-39 -0.7631409579451045 1107 40-41 -0.7918819813031419 1107 42-43 -0.7401805754854109 1107 44-45 -0.8049226686598345 1107 46-47 -0.8924908130626292 1107 48-49 -0.912550754412301 1107 50-51 -0.8710307402668107 1107 52-53 -0.8546500959257486 1107 54-55 -0.8087415068188122 1107 56-57 -0.8663713559565025 1107 58-59 -0.8430600438999889 1107 60-61 -0.9935661931507056 1107 62-63 -0.9280622287871481 1107 64-65 -0.8821733736121402 1107 66-67 -0.8560815051351298 1107 68-69 -0.8144714846909409 1107 70-71 -0.7666163002145794 1107 72-73 -0.8078083091462887 1107 74-75 -0.7890306762649182 1107 76-77 -0.7918400064551356 1107 78-79 -0.6587475547421491 1107 80-81 -0.6096539479876526 1107 82-83 -0.6409370962698446 1107 84-85 -0.7189210228574119 1107 86-87 -0.7461153865414119 1107 88-89 -0.6187930306824647 1107 90-91 -0.6284221758676409 1107 92-93 -0.6385655022483157 1107 94-95 -0.7347241945367884 1107 96-97 -0.7040435617451557 1107 98-99 -0.7828016313910453 1107 100 -0.7402182124310457 1108 1 -0.4360194515855582 1108 2 -0.4257292392367731 1108 3 -0.45366139967814334 1108 4 -0.26903029402206613 1108 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1.1534915332109392 2205 72-73 1.157543661145624 2205 74-75 1.203703948980369 2205 76-77 1.1444484142196565 2205 78-79 1.0496853640548665 2205 80-81 0.977262060910185 2205 82-83 0.9131193726490494 2205 84-85 0.9191100382728479 2205 86-87 0.9935182083571164 2205 88-89 0.9914868461834914 2205 90-91 1.0385280517312374 2205 92-93 0.9813170282670391 2205 94-95 1.06091963372333 2205 96-97 0.9995429521186416 2205 98-99 1.0073308723087955 2205 100 1.175177539009809 2206 1 0.6768894308271953 2206 2 0.6786520526850097 2206 3 0.6335384103361577 2206 4 0.27498950494005214 2206 5 0.4577175314071411 2206 6 0.5053299746817714 2206 7 0.5681008972071169 2206 8 0.5523066691463114 2206 9 0.7286561806042329 2206 10-11 0.7489026442301707 2206 12-13 0.7990504487726753 2206 14-15 0.9415611170176561 2206 16-17 0.9352728843280929 2206 18-19 0.8884697059700599 2206 20-21 0.9288232032796273 2206 22-23 0.9342336586110065 2206 24-25 0.9857806725739025 2206 26-27 1.047864813546859 2206 28-29 1.0474892487206446 2206 30-31 1.1365687853023232 2206 32-33 1.1985946797883855 2206 34-35 1.1911601337326516 2206 36-37 1.2069891107948294 2206 38-39 1.1684536316142058 2206 40-41 1.1928576123798749 2206 42-43 1.2904123343544995 2206 44-45 1.3018932502774092 2206 46-47 1.2989145673175315 2206 48-49 1.3236501479628018 2206 50-51 1.300117636578257 2206 52-53 1.3126924170540164 2206 54-55 1.286319107090577 2206 56-57 1.3003060154040895 2206 58-59 1.3064492398222853 2206 60-61 1.2815090674015508 2206 62-63 1.31131950566688 2206 64-65 1.3320292734970067 2206 66-67 1.3543763385567615 2206 68-69 1.361241469771386 2206 70-71 1.395633579150072 2206 72-73 1.3696973057933732 2206 74-75 1.3639472822022682 2206 76-77 1.3981714873276623 2206 78-79 1.2279145145861747 2206 80-81 1.2183336796581798 2206 82-83 1.1755211259749814 2206 84-85 1.2107122220944717 2206 86-87 1.2921834610742593 2206 88-89 1.3257768101487883 2206 90-91 1.2767119176824728 2206 92-93 1.3597744181856584 2206 94-95 1.4200604978033162 2206 96-97 1.3442351933784558 2206 98-99 1.3538925826039154 2206 100 1.5883594317288505 2207 1 0.7208573337219804 2207 2 0.6697340434026984 2207 3 0.6533719400946438 2207 4 0.41145634883320525 2207 5 0.5242319359470997 2207 6 0.5629044095439042 2207 7 0.5910527306042397 2207 8 0.5758784413177338 2207 9 0.7044859078781727 2207 10-11 0.7109488074183687 2207 12-13 0.7731667647779474 2207 14-15 0.9721329465704116 2207 16-17 0.9308008325431061 2207 18-19 0.935238603644656 2207 20-21 0.9361164732799949 2207 22-23 0.9216169472315769 2207 24-25 0.9906456798179235 2207 26-27 0.9860154519097364 2207 28-29 0.9658406214890434 2207 30-31 1.0304358292062261 2207 32-33 1.0802238146306067 2207 34-35 1.074520640517079 2207 36-37 1.1168495162191263 2207 38-39 1.0815100505972026 2207 40-41 1.0895991473146296 2207 42-43 1.1440223470590425 2207 44-45 1.1755456485759765 2207 46-47 1.140266559937679 2207 48-49 1.1484809970735412 2207 50-51 1.1250733147271674 2207 52-53 1.1419955065132399 2207 54-55 1.1207093134678203 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0.9583949014854909 2208 16-17 0.9450348598462526 2208 18-19 0.950751271889068 2208 20-21 0.9489629139772617 2208 22-23 0.9429179961288909 2208 24-25 0.9744541786524294 2208 26-27 0.9959130880254747 2208 28-29 1.014074231641537 2208 30-31 1.0772716559914386 2208 32-33 1.1012140090983138 2208 34-35 1.0849869286776723 2208 36-37 1.0937337123159026 2208 38-39 1.1445397594630506 2208 40-41 1.1344464337702433 2208 42-43 1.1616570551564038 2208 44-45 1.1738902036457333 2208 46-47 1.1656888440603481 2208 48-49 1.143549448706203 2208 50-51 1.2147068753451649 2208 52-53 1.2590206115413167 2208 54-55 1.2460760782296205 2208 56-57 1.245919177540621 2208 58-59 1.2716634036307752 2208 60-61 1.2435737788154526 2208 62-63 1.26836326247372 2208 64-65 1.316798877096602 2208 66-67 1.339947205306494 2208 68-69 1.3556178695074692 2208 70-71 1.3382250720113475 2208 72-73 1.3516737886946757 2208 74-75 1.3361741031200083 2208 76-77 1.3469371522980857 2208 78-79 1.195192967650847 2208 80-81 1.194623999443806 2208 82-83 1.1891232223868826 2208 84-85 1.1771071871648147 2208 86-87 1.1785552167003743 2208 88-89 1.2520681471080906 2208 90-91 1.3527198295268796 2208 92-93 1.3831597963165656 2208 94-95 1.3777302004311345 2208 96-97 1.390042248793641 2208 98-99 1.48574644830126 2208 100 1.6558206832710276 2209 1 0.6682493098419258 2209 2 0.6280078916566296 2209 3 0.5978037156300999 2209 4 0.37508880421114554 2209 5 0.4997833407280723 2209 6 0.4863273124430876 2209 7 0.5313982140281865 2209 8 0.5445527052332579 2209 9 0.7102415942648648 2209 10-11 0.6980297348917404 2209 12-13 0.7361421817083311 2209 14-15 0.9340029253298923 2209 16-17 0.912854289345951 2209 18-19 0.8972395861299205 2209 20-21 0.9287000550794602 2209 22-23 0.9370382051450079 2209 24-25 0.9417122322140656 2209 26-27 0.9702100159691653 2209 28-29 1.0164079458541622 2209 30-31 1.0380249845670306 2209 32-33 1.0393137691568128 2209 34-35 0.998268051451042 2209 36-37 1.025251583125069 2209 38-39 1.0189932661893337 2209 40-41 1.042795129002215 2209 42-43 1.0721728629150107 2209 44-45 1.0494423013440226 2209 46-47 1.0292848831628092 2209 48-49 1.063231524445321 2209 50-51 1.0854778013188664 2209 52-53 1.11376269585206 2209 54-55 1.0515716206718722 2209 56-57 1.0472194280842118 2209 58-59 1.102832889265244 2209 60-61 1.1395102824742978 2209 62-63 1.07780671362476 2209 64-65 1.1731386467184706 2209 66-67 1.2079080621949387 2209 68-69 1.1962042805691908 2209 70-71 1.187679652241286 2209 72-73 1.2318210972993668 2209 74-75 1.2322036084034167 2209 76-77 1.2045397172278776 2209 78-79 1.051082809825445 2209 80-81 1.0429033689693128 2209 82-83 1.010412644970046 2209 84-85 1.0326951223471426 2209 86-87 1.057586007246286 2209 88-89 1.0863338916799066 2209 90-91 1.1595819675846535 2209 92-93 1.1469757070721194 2209 94-95 1.2136566438473189 2209 96-97 1.1912921408421688 2209 98-99 1.1944703776143122 2209 100 1.3773339766266481 2210 1 0.6584236829302696 2210 2 0.6355998884842293 2210 3 0.5930637540654615 2210 4 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1.156339303887755 2210 68-69 1.1677858017987433 2210 70-71 1.1718795081184652 2210 72-73 1.2083629763765913 2210 74-75 1.2536523278362992 2210 76-77 1.2333875893584043 2210 78-79 1.029374156385927 2210 80-81 0.9984908548665388 2210 82-83 0.9928211013184836 2210 84-85 1.0667648918539427 2210 86-87 1.0981995980930606 2210 88-89 1.1033288582708138 2210 90-91 1.2023184354218053 2210 92-93 1.2131506386587354 2210 94-95 1.237748771434216 2210 96-97 1.2681521910941704 2210 98-99 1.3557780562702355 2210 100 1.6142200592306715 2211 1 0.6719285215510666 2211 2 0.6424234346293005 2211 3 0.5902545064616405 2211 4 0.400256159127359 2211 5 0.5188029883957981 2211 6 0.5025260944786751 2211 7 0.3962583927747616 2211 8 0.5000620176732511 2211 9 0.714349219817322 2211 10-11 0.7030875415315734 2211 12-13 0.7584554976136744 2211 14-15 0.9299273930284642 2211 16-17 0.9236292015284633 2211 18-19 0.828144517941908 2211 20-21 0.8627910116043864 2211 22-23 0.9170335120764364 2211 24-25 0.9708432416684261 2211 26-27 0.9478650795843393 2211 28-29 1.003030930132276 2211 30-31 0.990938774309555 2211 32-33 1.0983252344949008 2211 34-35 0.9712247390344118 2211 36-37 1.0736234628225034 2211 38-39 1.1288531714875987 2211 40-41 1.1118841857447137 2211 42-43 1.1889945432050055 2211 44-45 1.1329898300960508 2211 46-47 1.1955220742632022 2211 48-49 1.1898188393870086 2211 50-51 1.2231401210455601 2211 52-53 1.2102479147773124 2211 54-55 1.207473297607045 2211 56-57 1.1805179456465638 2211 58-59 1.1689350892500059 2211 60-61 1.219985320702527 2211 62-63 1.1900008300884508 2211 64-65 1.2790248105352902 2211 66-67 1.3303883899982978 2211 68-69 1.2930462315086686 2211 70-71 1.3098365974043986 2211 72-73 1.2860992153146853 2211 74-75 1.2732163503489105 2211 76-77 1.2512414468870823 2211 78-79 1.1450293904779585 2211 80-81 1.125884986728117 2211 82-83 1.1056392804690347 2211 84-85 1.1597116758941866 2211 86-87 1.2226850508839746 2211 88-89 1.2438039623422057 2211 90-91 1.2831882060046844 2211 92-93 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1.1374049212807407 2310 82-83 1.1480481455910194 2310 84-85 1.1593532934706232 2310 86-87 1.2544735523706763 2310 88-89 1.2923855339887211 2310 90-91 1.3413873990388616 2310 92-93 1.3796312133270412 2310 94-95 1.4431034768445024 2310 96-97 1.5592675332963246 2310 98-99 1.5051898206035297 2310 100 1.630406896103743 2311 1 0.37334193714092123 2311 2 0.4504250571872035 2311 3 0.47810713151409345 2311 4 0.30689530737517146 2311 5 -0.03085783295664868 2311 6 0.1904313138614313 2311 7 0.4096644277262058 2311 8 0.16932310845380982 2311 9 0.3228031880430109 2311 10-11 0.5029549737091017 2311 12-13 0.5144476384649437 2311 14-15 0.6146362103617946 2311 16-17 0.8246085711298505 2311 18-19 0.8511176751061384 2311 20-21 0.8968929763246294 2311 22-23 0.8340558327943555 2311 24-25 0.800639526140877 2311 26-27 0.8202941078403541 2311 28-29 0.7996345233122142 2311 30-31 0.8981346727113788 2311 32-33 1.012099321148959 2311 34-35 0.9901485644709638 2311 36-37 1.0469070751734648 2311 38-39 1.063389391901481 2311 40-41 1.0333107966397321 2311 42-43 1.1496403929197 2311 44-45 1.0813876887316454 2311 46-47 1.1678845925089192 2311 48-49 1.1316779336595815 2311 50-51 1.1703208324212397 2311 52-53 1.1871834470631342 2311 54-55 1.1934640153320757 2311 56-57 1.1884717212713696 2311 58-59 1.2197205485092582 2311 60-61 1.177071309003523 2311 62-63 1.2096432031236475 2311 64-65 1.2268446479450645 2311 66-67 1.2451389581968542 2311 68-69 1.2255405314898553 2311 70-71 1.2361826183969953 2311 72-73 1.2415996344244746 2311 74-75 1.1262718892677057 2311 76-77 1.03753875835476 2311 78-79 1.0063841652611671 2311 80-81 1.0268029817051385 2311 82-83 1.0805534461613178 2311 84-85 1.0725252001536774 2311 86-87 1.1796663437072041 2311 88-89 1.1705525318797072 2311 90-91 1.2177168203368502 2311 92-93 1.3246202715706588 2311 94-95 1.4195583933319575 2311 96-97 1.4357940807222391 2311 98-99 1.4808006547365444 2311 100 1.5605428915106145 2312 1 0.13159837919156558 2312 2 0.09647348481684048 2312 3 0.29266182400576213 2312 4 0.23751713814896647 2312 5 0.30356163387730817 2312 6 0.38631395670284263 2312 7 0.4343330556243927 2312 8 0.3214495014992593 2312 9 0.4489114281153803 2312 10-11 0.30710677845325307 2312 12-13 0.4594804050304262 2312 14-15 0.8221183578263407 2312 16-17 0.857466630892695 2312 18-19 0.8851970498832245 2312 20-21 0.8686288704629774 2312 22-23 0.512316536706642 2312 24-25 0.48682795624295494 2312 26-27 0.7872326492048884 2312 28-29 0.9593578207508315 2312 30-31 0.8815185079957644 2312 32-33 0.7658949942007851 2312 34-35 0.9379829091874328 2312 36-37 0.9851632027837312 2312 38-39 1.0138861745563688 2312 40-41 0.981390016806607 2312 42-43 0.8244431399271832 2312 44-45 0.7843684571604044 2312 46-47 0.9674748989293391 2312 48-49 1.061745823919587 2312 50-51 1.1083701882092711 2312 52-53 1.1340364360640507 2312 54-55 1.1413433407653528 2312 56-57 1.1447978239213938 2312 58-59 1.1555832076082098 2312 60-61 1.1731607865000058 2312 62-63 1.1789233655510927 2312 64-65 1.2423122594537404 2312 66-67 1.2723136324092863 2312 68-69 1.2265705136077685 2312 70-71 1.1012221670423443 2312 72-73 1.1684002827047024 2312 74-75 1.2100064609060084 2312 76-77 1.158876518774779 2312 78-79 1.084600708735877 2312 80-81 0.9597816515171047 2312 82-83 1.0093805087037708 2312 84-85 0.8980137454925483 2312 86-87 1.070146891185324 2312 88-89 0.7281705135828886 2312 90-91 1.0989079642651411 2312 92-93 1.2464081850846114 2312 94-95 1.3223775529395745 2312 96-97 1.4697498508785571 2312 98-99 1.531029712192602 2312 100 1.6480951553128627 2313 1 -0.42615574030969583 2313 2 -0.061677118134113584 2313 3 0.2444534069902886 2313 4 0.10389213531810526 2313 5 0.23860338103127532 2313 6 0.3806941130244468 2313 7 0.1253803850818116 2313 8 0.03438785721996851 2313 9 0.24583308989803498 2313 10-11 0.36392948092261435 2313 12-13 0.5714679673699621 2313 14-15 0.6053450513408549 2313 16-17 0.7611731715753152 2313 18-19 0.6178932672487392 2313 20-21 0.7982439782490189 2313 22-23 0.8756659245053129 2313 24-25 0.728032647447094 2313 26-27 0.8947362649152595 2313 28-29 0.8140550487224587 2313 30-31 0.833723684727012 2313 32-33 0.980162897945128 2313 34-35 0.987426276944511 2313 36-37 0.8290633293846454 2313 38-39 0.601967151602814 2313 40-41 0.7107802119169762 2313 42-43 0.7943463177132557 2313 44-45 0.9695692627020875 2313 46-47 1.055642034378316 2313 48-49 1.086436563535102 2313 50-51 1.016124472396541 2313 52-53 0.964559012194357 2313 54-55 1.052673609520447 2313 56-57 1.1525370095041225 2313 58-59 1.039791882556763 2313 60-61 1.044995056780813 2313 62-63 1.0661669070652025 2313 64-65 1.1271093169490882 2313 66-67 1.157973276366583 2313 68-69 1.1579709336941733 2313 70-71 1.0876522578540317 2313 72-73 1.1828045771539877 2313 74-75 0.8333796776454605 2313 76-77 1.006873532141089 2313 78-79 0.8157760013387687 2313 80-81 0.9170826976360686 2313 82-83 1.0080999266391686 2313 84-85 1.0317181181162098 2313 86-87 1.1012958217515276 2313 88-89 1.085812958237696 2313 90-91 1.2326784397401234 2313 92-93 1.0413827297075002 2313 94-95 1.1243683456480902 2313 96-97 1.3565362574373374 2313 98-99 1.4627883976629867 2313 100 1.5131919298940133 2314 1 -0.38191901244797677 2314 2 -0.18462911040838392 2314 3 0.10051295115123082 2314 4 -0.16292652303225452 2314 5 0.15931658196558374 2314 6 0.17747774139798622 2314 7 0.10925013490128066 2314 8 0.1635815894850552 2314 9 0.24053959967329774 2314 10-11 0.41132376841379426 2314 12-13 0.49951710884941036 2314 14-15 0.611029100948997 2314 16-17 0.7785903893760278 2314 18-19 0.8103821599362462 2314 20-21 0.43572590977117187 2314 22-23 0.6531145295381506 2314 24-25 0.8598474589639764 2314 26-27 0.841778500752099 2314 28-29 0.7932399861349992 2314 30-31 0.7555640449810141 2314 32-33 0.8543239846458093 2314 34-35 0.9115643391866755 2314 36-37 0.8429908905867691 2314 38-39 0.8757043566681304 2314 40-41 0.9141609072874743 2314 42-43 0.7860401460639324 2314 44-45 0.806666612175789 2314 46-47 0.8745919254750234 2314 48-49 0.7763251036512244 2314 50-51 0.9368145465796474 2314 52-53 1.053163664063888 2314 54-55 1.0724904313498698 2314 56-57 0.9164531016782576 2314 58-59 0.8224400009730957 2314 60-61 0.9164837258876659 2314 62-63 0.6852988572705101 2314 64-65 0.8249445312129495 2314 66-67 0.9301931599818829 2314 68-69 1.0303475164423759 2314 70-71 1.06707138589924 2314 72-73 1.054631176229627 2314 74-75 1.1243023331227278 2314 76-77 0.8727550234247197 2314 78-79 0.9262085357424894 2314 80-81 0.9198575039054973 2314 82-83 0.8947346579968567 2314 84-85 0.7806908331910449 2314 86-87 0.9321183764662067 2314 88-89 1.028993837683366 2314 90-91 1.1261088739130614 2314 92-93 1.183557471665626 2314 94-95 1.3259410064409316 2314 96-97 1.3954733873079377 2314 98-99 1.434098573669882 2314 100 1.4452870279688845 2315 1 -0.06476375278958457 2315 2 0.11334972376150887 2315 3 0.1258097938152929 2315 4 -0.07882845791063886 2315 5 0.23236616931844623 2315 6 0.35637303637249573 2315 7 0.4188468511422059 2315 8 0.3741985250080617 2315 9 0.5930074363494526 2315 10-11 0.5593666277484957 2315 12-13 0.648935869212302 2315 14-15 0.7177707582956074 2315 16-17 0.7478869351016542 2315 18-19 0.7919063572269351 2315 20-21 0.797804564736218 2315 22-23 0.8877767469167352 2315 24-25 0.8074087605561004 2315 26-27 0.8958326204226594 2315 28-29 0.9617990011701281 2315 30-31 0.9196952289434677 2315 32-33 0.940834363478892 2315 34-35 0.9207667601439056 2315 36-37 0.9555518063546984 2315 38-39 0.9794186181496869 2315 40-41 0.9281171763913676 2315 42-43 1.0119865321819006 2315 44-45 0.8781652724617857 2315 46-47 0.9087286025135128 2315 48-49 1.00068711790599 2315 50-51 0.9531010084495932 2315 52-53 0.9595005460225678 2315 54-55 0.8749312707044439 2315 56-57 1.014892563203162 2315 58-59 1.032442042343888 2315 60-61 1.101223798849439 2315 62-63 1.0746969450022092 2315 64-65 1.1687836396103322 2315 66-67 1.1379710348792997 2315 68-69 1.0545190347384334 2315 70-71 0.737643292759131 2315 72-73 1.027610156748871 2315 74-75 1.169951293804779 2315 76-77 1.0843336605328666 2315 78-79 0.9542588270956784 2315 80-81 1.023731235932658 2315 82-83 1.0159264673007655 2315 84-85 0.897550539777555 2315 86-87 0.9452793803056423 2315 88-89 1.1122577244973044 2315 90-91 1.15785287041815 2315 92-93 1.2237220821688162 2315 94-95 1.331133588210072 2315 96-97 1.365110202556611 2315 98-99 1.346152000471097 2315 100 1.2647264816431125 2316 1 0.4877903659254059 2316 2 0.4747594176305512 2316 3 0.47793084527233276 2316 4 0.30140846380029984 2316 5 0.4115961014878877 2316 6 0.4121847023389691 2316 7 0.4701408447109685 2316 8 0.48085469372912826 2316 9 0.534843002243413 2316 10-11 0.46766999338624515 2316 12-13 0.6366357342818247 2316 14-15 0.7786116221365447 2316 16-17 0.8075901357096669 2316 18-19 0.8067325844072855 2316 20-21 0.8744566593352587 2316 22-23 0.8774697296585003 2316 24-25 0.9513807896832915 2316 26-27 0.9887908983953224 2316 28-29 0.9335527399514021 2316 30-31 0.8398718727087484 2316 32-33 0.8153741409667035 2316 34-35 0.7241222483357035 2316 36-37 0.7004569349039613 2316 38-39 0.634161012129205 2316 40-41 0.7604867445264887 2316 42-43 0.9168572062068705 2316 44-45 0.874488981707664 2316 46-47 0.9128246215483173 2316 48-49 0.6744018261218159 2316 50-51 0.7515766419437924 2316 52-53 0.9135622995469248 2316 54-55 1.0170891639783264 2316 56-57 1.073033410286989 2316 58-59 0.9217204335342544 2316 60-61 0.9870284331830916 2316 62-63 1.0306384333036007 2316 64-65 1.0142841776644644 2316 66-67 0.9730188815906544 2316 68-69 0.9616062249205157 2316 70-71 0.8917345673875161 2316 72-73 0.9138909153773866 2316 74-75 0.9684730593847206 2316 76-77 0.8049709231040829 2316 78-79 0.7722453181647779 2316 80-81 0.8179425613565314 2316 82-83 0.7634975551707583 2316 84-85 0.8208983498774103 2316 86-87 0.8088672411692102 2316 88-89 0.7301624249857994 2316 90-91 0.8500680952453585 2316 92-93 0.9059164471736203 2316 94-95 0.8746551004479279 2316 96-97 1.0370537271248352 2316 98-99 1.0936230380697651 2316 100 0.7136803884719924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 13.0 12 67.0 13 253.0 14 1088.0 15 3256.0 16 7825.0 17 15590.0 18 25587.0 19 36648.0 20 50465.0 21 67793.0 22 80876.0 23 85441.0 24 88882.0 25 97808.0 26 113741.0 27 139572.0 28 170148.0 29 207973.0 30 254078.0 31 313766.0 32 395644.0 33 510766.0 34 707767.0 35 977010.0 36 1481554.0 37 2221220.0 38 1901787.0 39 281635.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9458758834091 19.691988902899197 12.922606917677543 24.439528296014156 2 16.136576673474767 20.792421158488043 39.10392093682023 23.967081231216962 3 18.719751427313128 25.74794450598357 30.518446776235063 25.013857290468245 4 12.767655107011247 16.863155801168872 37.927577067517134 32.44161202430275 5 13.03352673583392 37.68060974288743 34.901999816563745 14.383863704714908 6 30.741013174754816 37.26515738512269 17.860692207187274 14.133137232935217 7 26.848779956084417 32.68437986501442 22.50098869155646 17.9658514873447 8 26.009778812936617 35.25982987143222 20.249354943912817 18.48103637171835 9 26.615417554201485 15.184512823478116 20.38969941285958 37.81037020946082 10-11 25.153693738500195 25.824176629175117 28.45641692724261 20.565712705082078 12-13 25.45626884719301 23.71977782947926 28.27065765654366 22.55329566678407 14-15 22.85312363029462 25.347657017836234 25.846099750568634 25.95311960130051 16-17 22.199858990034805 27.93773975881797 26.87588207534984 22.986519175797387 18-19 21.867175514512038 27.370060614596593 28.814881543241633 21.947882327649737 20-21 22.73908089357092 26.773205921456427 28.529187290223803 21.958525894748853 22-23 22.756158937447772 26.49489778177844 28.292424166062073 22.45651911471171 24-25 22.436088899272573 26.476448592361766 28.654626107265628 22.43283640110003 26-27 22.395087969751202 26.638740133608223 28.413494164847027 22.552677731793544 28-29 22.06708153133979 27.203815554260625 28.41633361652119 22.312769297878397 30-31 22.73805054089565 26.732989496985994 28.46075346808874 22.068206494029617 32-33 22.759728754649558 26.772215008365603 28.07772307077636 22.390333166208478 34-35 22.763603666057897 26.751605175247956 28.484174508795174 22.000616649898973 36-37 22.51956465008052 26.570206119703542 28.428607285331587 22.48162194488435 38-39 22.451133807427702 26.606017964838184 28.561491167929404 22.381357059804703 40-41 22.38849645890003 26.068364944538747 28.95317750595352 22.58996109060771 42-43 22.12844680809604 26.40803405822762 28.512806582970583 22.950712550705752 44-45 21.83445427258934 26.727413344651396 28.689830763854513 22.748301618904748 46-47 21.8451101670502 26.40284430884735 28.44538898293354 23.30665654116891 48-49 22.039875609228876 26.4426650147391 28.431825314520193 23.085634061511836 50-51 21.793194468437783 26.5243535681794 28.30774361370593 23.374708349676887 52-53 21.515857740107553 26.772814761202202 28.162514324722494 23.54881317396775 54-55 21.358574760357296 26.530990911296744 28.36394549982007 23.74648882852589 56-57 21.76358221236091 26.408880613823854 27.90925383175949 23.91828334205575 58-59 21.362015263005873 26.544784224717272 28.114459943229907 23.97874056904695 60-61 21.595764610552294 26.64005304101908 27.793287904393328 23.9708944440353 62-63 21.51909070260023 26.442260475618223 27.665723742882104 24.372925078899442 64-65 21.078066444201596 26.335817402342148 27.756150902613026 24.82996525084323 66-67 21.366852140710122 26.353134331537614 27.428377616592588 24.851635911159683 68-69 21.22123809571276 26.49014249621534 27.443057168430464 24.845562239641435 70-71 21.184588781789586 26.29823017017296 27.40596601837222 25.11121502966524 72-73 21.281015537631966 26.56669290226823 27.16501118555117 24.987280374548632 74-75 21.477877541003153 26.68655450399283 26.911533465492294 24.92403448951172 76-77 21.47031207431738 26.564705809939888 26.806831214241438 25.158150901501298 78-79 21.31324510078013 26.655566776249145 26.81842150043792 25.212766622532808 80-81 21.12020433503816 26.91786429838581 26.742221142376444 25.219710224199588 82-83 21.429947239147342 26.46273769360818 26.994527735242098 25.11278733200238 84-85 21.649851685401163 26.542340152304934 26.491976070514013 25.31583209177989 86-87 21.225928436213653 26.783099609476537 26.67166107519619 25.319310879113623 88-89 21.37284211472757 26.297042485320908 26.698987709436057 25.63112769051547 90-91 21.470624673784986 26.123357004826858 26.683279246696618 25.722739074691535 92-93 21.514835433208834 26.076476947499117 26.73878489258104 25.66990272671101 94-95 21.091469984475307 25.922078799925043 27.01630432367164 25.970146891928014 96-97 21.17032968133167 25.795921166070997 26.986370204870596 26.04737894772674 98-99 21.081463434840156 25.967142723720567 26.83961982128224 26.111774020157036 100 21.09612626154722 25.928410538181666 26.867418126734567 26.108045073536545 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1499.0 1 1122.0 2 1240.5 3 1799.5 4 1521.5 5 1318.0 6 1958.0 7 3331.0 8 4952.0 9 5967.5 10 5696.5 11 5207.5 12 5662.5 13 6512.5 14 7499.5 15 8551.0 16 9562.5 17 10889.0 18 12310.5 19 13653.5 20 15157.5 21 17083.0 22 19761.0 23 23695.5 24 28922.0 25 35011.0 26 42924.5 27 52950.0 28 64577.5 29 78716.0 30 95450.5 31 112610.5 32 133953.0 33 155317.5 34 174774.5 35 195677.0 36 218028.5 37 246699.5 38 265246.0 39 271314.5 40 278386.0 41 286831.5 42 300536.0 43 319429.5 44 342782.5 45 374144.0 46 402900.5 47 427508.5 48 450611.5 49 461100.5 50 461477.5 51 460874.0 52 448685.5 53 422608.5 54 395697.5 55 358161.5 56 318171.0 57 276213.0 58 232579.5 59 198537.5 60 161163.5 61 124462.0 62 96765.0 63 74631.0 64 54715.5 65 38322.5 66 27765.0 67 21261.0 68 15981.5 69 11853.5 70 9280.0 71 7165.0 72 5421.5 73 4277.0 74 3340.0 75 2433.5 76 1707.0 77 1107.5 78 714.5 79 458.0 80 281.0 81 185.5 82 123.5 83 76.5 84 62.0 85 43.5 86 25.0 87 18.5 88 14.5 89 8.0 90 5.0 91 4.5 92 3.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052254804585100276 2 0.0 3 0.0 4 0.013918335800704276 5 0.003945970290164582 6 0.0 7 1.367415447086736E-4 8 3.516211149651607E-4 9 0.001924148879114907 10-11 7.325439895107514E-4 12-13 3.0278484899777725E-4 14-15 5.371989256412177E-5 16-17 9.278890533802852E-5 18-19 0.0 20-21 9.767253193476686E-6 22-23 9.767253193476686E-6 24-25 8.790527874129017E-5 26-27 9.767253193476686E-6 28-29 9.278890533802851E-4 30-31 8.692855342194251E-4 32-33 0.014020891959235783 34-35 0.02284560521954197 36-37 0.030512898976421166 38-39 0.020267050376464122 40-41 1.904614372727954E-4 42-43 0.001753221948229065 44-45 0.009479119224269123 46-47 0.013967172066671661 48-49 0.005430592775573038 50-51 0.010514448062777652 52-53 0.015822950173432233 54-55 0.009464468344478907 56-57 0.0029985467303973424 58-59 0.00839983774638995 60-61 0.001748338321632327 62-63 0.0010011434523313602 64-65 0.018845915036813266 66-67 0.04410891542174072 68-69 0.05401291015992608 70-71 0.04738582886815214 72-73 0.04012387611880223 74-75 0.028242012608937838 76-77 0.028271314368518263 78-79 0.01225790275781324 80-81 0.0020071705312594587 82-83 0.027040640466140204 84-85 0.04697560423402612 86-87 0.047361410735168454 88-89 0.05912118358011438 90-91 0.03398027386010539 92-93 0.025849035576536047 94-95 0.007154512964221673 96-97 0.0038238796252461228 98-99 0.022122828483224694 100 0.019085212740053443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0238293E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.645531926362146 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20635861132217 41.62373950615327 2 15.987587818916149 18.432260080816988 3 5.489049392790697 9.492575160524847 4 2.378731798446161 5.484930393263241 5 1.211341359990093 3.4914208520515926 6 0.7252771284006195 2.5085391516406905 7 0.46229994863768675 1.8654668514124548 8 0.30495044890461304 1.4063224670631473 9 0.2195098326767594 1.1388384960946691 >10 0.9562806231181137 9.4784482051046 >50 0.04105788891300504 1.5915646923479472 >100 0.016029891790680456 1.599489034340807 >500 8.716850842119319E-4 0.35136940557572394 >1k 5.664282240367554E-4 0.5954101928788458 >5k 2.1785696265783932E-5 0.0692097860149592 >10k+ 6.53570887973518E-5 0.8704157247161689 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28807 0.2813652627444829 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25650 0.250530044412677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14852 0.14506324442951574 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.767253193476686E-6 2 0.0 0.0 0.0 9.767253193476686E-6 9.767253193476686E-6 3 0.0 0.0 0.0 9.767253193476686E-6 9.767253193476686E-6 4 0.0 0.0 0.0 9.767253193476686E-6 9.767253193476686E-6 5 0.0 0.0 0.0 9.767253193476686E-6 9.767253193476686E-6 6 0.0 0.0 0.0 9.767253193476686E-6 3.9069012773906744E-5 7 0.0 0.0 0.0 1.9534506386953372E-5 3.9069012773906744E-5 8 0.0 0.0 0.0 1.9534506386953372E-5 6.83707723543368E-5 9 0.0 0.0 0.0 4.883626596738343E-5 7.813802554781349E-5 10-11 0.0 0.0 0.0 7.325439895107514E-5 7.813802554781349E-5 12-13 0.0 9.767253193476686E-6 0.0 7.813802554781349E-5 1.2697429151519693E-4 14-15 0.0 2.9301759580430058E-5 0.0 1.0743978512824355E-4 1.5139242449888865E-4 16-17 0.0 3.9069012773906744E-5 0.0 1.367415447086736E-4 1.904614372727954E-4 18-19 0.0 3.9069012773906744E-5 0.0 2.246468234499638E-4 2.1976319685322544E-4 20-21 0.0 3.9069012773906744E-5 0.0 4.737117798836193E-4 2.7836671601408553E-4 22-23 9.767253193476686E-6 3.9069012773906744E-5 0.0 0.0012404411555715393 2.9301759580430055E-4 24-25 9.767253193476686E-6 7.325439895107514E-5 0.0 0.002998546730397343 3.0278484899777725E-4 26-27 9.767253193476686E-6 9.767253193476686E-5 0.0 0.007403577920655328 3.467374883684224E-4 28-29 9.767253193476686E-6 1.0743978512824355E-4 0.0 0.026439954394741387 3.6627199475537577E-4 30-31 9.767253193476686E-6 1.1232341172498188E-4 0.0 0.09689603530588546 3.711556213521141E-4 32-33 9.767253193476686E-6 1.1720703832172023E-4 0.0 0.2575673503385769 3.711556213521141E-4 34-35 9.767253193476686E-6 1.1720703832172023E-4 0.0 0.5207752893963866 3.8092287454559074E-4 36-37 9.767253193476686E-6 1.2209066491845858E-4 0.0 0.9307606258191674 3.8092287454559074E-4 38-39 9.767253193476686E-6 1.2697429151519693E-4 0.0 1.4900921471968032 3.9069012773906744E-4 40-41 9.767253193476686E-6 1.3185791811193525E-4 0.0 2.054111950107308 3.9069012773906744E-4 42-43 9.767253193476686E-6 1.6604330428910368E-4 0.0 2.591750402142232 3.9069012773906744E-4 44-45 9.767253193476686E-6 1.7581055748258035E-4 0.0 3.2464884527137485 4.0045738093254414E-4 46-47 9.767253193476686E-6 1.8557781067605705E-4 0.0 4.012749000248381 4.1510826072275916E-4 48-49 9.767253193476686E-6 1.8557781067605705E-4 0.0 4.780225570805602 4.199918873194975E-4 50-51 9.767253193476686E-6 1.8557781067605705E-4 0.0 5.529886671537922 4.5906090009340423E-4 52-53 9.767253193476686E-6 1.8557781067605705E-4 0.0 6.243032896206428 4.6882815328688093E-4 54-55 9.767253193476686E-6 1.904614372727954E-4 0.0 7.028676557703516 4.8347903307709595E-4 56-57 9.767253193476686E-6 1.9534506386953372E-4 0.0 7.896365146025808 4.932462862705727E-4 58-59 9.767253193476686E-6 1.9534506386953372E-4 0.0 8.828610394330383 4.98129912867311E-4 60-61 9.767253193476686E-6 2.2953045004670214E-4 0.0 9.730962964236323 5.030135394640493E-4 62-63 9.767253193476686E-6 2.4418132983691716E-4 0.0 10.606411635220832 5.371989256412178E-4 64-65 9.767253193476686E-6 2.490649564336555E-4 0.0 11.4678491814993 5.42082552237956E-4 66-67 9.767253193476686E-6 2.734830894173472E-4 0.0 12.420351712927145 5.567334320281711E-4 68-69 9.767253193476686E-6 2.734830894173472E-4 0.0 13.443696131767279 5.665006852216478E-4 70-71 9.767253193476686E-6 2.7836671601408553E-4 0.0 14.474204830824826 5.762679384151245E-4 72-73 9.767253193476686E-6 2.832503426108239E-4 0.0 15.443736568195499 6.397550841727229E-4 74-75 9.767253193476686E-6 2.832503426108239E-4 0.0 16.36182418299613 6.934749767368447E-4 76-77 9.767253193476686E-6 2.832503426108239E-4 0.0 17.3412403805986 7.227767363172748E-4 78-79 9.767253193476686E-6 2.881339692075623E-4 0.0 18.368638209514028 7.423112427042282E-4 80-81 9.767253193476686E-6 2.930175958043006E-4 0.0 19.396031154802856 7.569621224944432E-4 82-83 9.767253193476686E-6 3.027848489977773E-4 0.0 20.41224547881175 8.2533289484878E-4 84-85 9.767253193476686E-6 3.1255210219125395E-4 0.0 21.37516966939704 8.302165214455183E-4 86-87 9.767253193476686E-6 3.1255210219125395E-4 0.0 22.327198489045 8.302165214455183E-4 88 9.767253193476686E-6 3.1255210219125395E-4 0.0 23.059351788428014 8.302165214455183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15825 0.0 53.2439 1 GTATCAA 27835 0.0 38.615406 1 CAACGCA 32885 0.0 31.807009 5 ATCAACG 33040 0.0 31.57724 3 TACCTGG 6900 0.0 30.717402 2 TATCAAC 34835 0.0 30.624363 2 TCAACGC 35095 0.0 29.83579 4 AACGCAG 35660 0.0 29.73858 6 GTGGTAT 6195 0.0 27.323526 1 GTACATG 32520 0.0 26.979671 1 ACGCAGA 39875 0.0 26.194422 7 TACATGG 33010 0.0 26.039051 2 CGCAGAG 40720 0.0 25.523796 8 GTACTAG 4505 0.0 25.466578 1 ACATGGG 32760 0.0 25.032995 3 CATGGGG 25080 0.0 23.891878 4 ACCTGGG 8810 0.0 23.151283 3 GCAGAGT 44730 0.0 22.9625 9 ATGGGGG 19315 0.0 22.79504 5 TGGGGGG 18570 0.0 22.620161 6 >>END_MODULE