FastQCFastQC Report
Mon 6 Jun 2016
ERR1211379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658333
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100340.21539894206790283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66790.1433774700091213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG49650.042.282612
GGTATCA71200.041.165931
GTATCAA124600.040.9579241
ACCTGGG56950.036.318773
CAACGCA150900.033.301265
ATCAACG151250.033.2241973
TCAACGC156300.032.180814
TATCAAC158000.032.0554162
GTACCTG67500.031.4847531
AACGCAG161900.031.4738566
CCTGGGG52400.028.348364
ACGCAGA180950.027.9265587
GTACATG182850.027.8585321
CGCAGAG185300.027.244438
TACATGG182650.027.136572
TATCACG5400.027.0217932
ACATGGG184700.025.8073393
TAACGCA5750.025.3434664
CATGGGG139450.024.776564
GCAGAGT204250.024.1874189
Produced by FastQC (version 0.11.5)