##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4658333 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56403760744455 31.0 31.0 34.0 30.0 34.0 2 31.64256011753561 33.0 31.0 34.0 30.0 34.0 3 31.71269743919123 33.0 31.0 34.0 30.0 34.0 4 35.32917848509327 37.0 35.0 37.0 33.0 37.0 5 35.383798238554434 37.0 35.0 37.0 33.0 37.0 6 35.440631015429766 37.0 35.0 37.0 33.0 37.0 7 35.38870986681287 37.0 35.0 37.0 33.0 37.0 8 35.38961469693128 37.0 35.0 37.0 33.0 37.0 9 36.99299813903386 39.0 37.0 39.0 33.0 39.0 10-11 36.919348187431 39.0 37.0 39.0 33.0 39.0 12-13 36.84300027499107 39.0 37.0 39.0 33.0 39.0 14-15 38.07229946850086 40.0 38.0 41.0 33.0 41.0 16-17 38.06140243730965 40.0 38.0 41.0 33.0 41.0 18-19 38.05581749952182 40.0 38.0 41.0 33.0 41.0 20-21 38.0415158169242 40.0 38.0 41.0 33.0 41.0 22-23 37.92865248147782 40.0 38.0 41.0 33.0 41.0 24-25 37.84231268996871 40.0 37.5 41.0 32.5 41.0 26-27 37.717550140790706 40.0 37.0 41.0 32.0 41.0 28-29 37.59936032911344 40.0 37.0 41.0 32.0 41.0 30-31 37.46214160730888 40.0 37.0 41.0 32.0 41.0 32-33 37.29219121947701 40.0 36.5 41.0 31.0 41.0 34-35 37.18891994196207 40.0 36.0 41.0 31.0 41.0 36-37 37.08362358380133 40.0 36.0 41.0 31.0 41.0 38-39 36.945340747430464 39.0 36.0 41.0 30.0 41.0 40-41 36.8054319002098 39.0 36.0 41.0 30.0 41.0 42-43 36.64034333741276 39.0 35.0 41.0 30.0 41.0 44-45 36.537896281781485 39.0 35.0 41.0 30.0 41.0 46-47 36.35840031187122 39.0 35.0 40.5 29.0 41.0 48-49 36.15103364229221 39.0 35.0 40.0 28.5 41.0 50-51 35.523182219905706 38.0 34.0 39.5 27.5 40.5 52-53 35.84149619617146 38.5 35.0 40.0 28.5 40.5 54-55 36.26369916019314 39.0 35.0 40.5 29.0 41.0 56-57 36.2038381541208 39.0 35.0 41.0 29.0 41.0 58-59 36.01692783663169 39.0 35.0 40.0 28.0 41.0 60-61 35.80097418969404 38.0 35.0 40.0 28.0 41.0 62-63 35.536254278086176 38.0 34.0 40.0 28.0 41.0 64-65 35.34625465805042 37.5 34.0 40.0 28.0 41.0 66-67 35.166180369673015 37.0 34.0 40.0 28.0 41.0 68-69 34.85469630445054 36.5 34.0 39.5 27.0 41.0 70-71 34.50063316641382 36.0 34.0 39.0 26.0 41.0 72-73 34.13078047447445 35.5 33.5 39.0 26.0 40.5 74-75 33.767661843839846 35.0 33.0 38.0 26.0 40.0 76-77 33.39511344508862 35.0 33.0 37.0 26.0 39.0 78-79 33.032799286783494 35.0 33.0 37.0 25.0 39.0 80-81 32.67952473556528 35.0 32.0 36.0 25.0 39.0 82-83 32.35169877293015 35.0 32.0 36.0 24.0 37.0 84-85 32.05094279434295 35.0 32.0 35.5 24.0 37.0 86-87 31.763462487546512 35.0 31.5 35.0 23.0 36.5 88-89 31.509159392426433 35.0 31.0 35.0 22.5 36.0 90-91 31.272641200188996 34.0 31.0 35.0 20.5 36.0 92-93 31.0557711310033 34.0 31.0 35.0 20.0 36.0 94-95 30.84453075810596 34.0 31.0 35.0 19.5 35.0 96-97 30.625694749602488 34.0 30.0 35.0 19.0 35.0 98-99 30.39662020727157 34.0 30.0 35.0 18.0 35.0 100 29.229414041460753 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3066356155810297 1101 2 -0.31695338621772606 1101 3 -0.415704606598819 1101 4 -0.2736766140418112 1101 5 -0.2601871644880518 1101 6 -0.287883360124944 1101 7 -0.23571104975846424 1101 8 -0.3139347085179196 1101 9 -0.39744864631118304 1101 10-11 -0.3638651760008713 1101 12-13 -0.38070451099487457 1101 14-15 -0.5462103290672857 1101 16-17 -0.5293943851076506 1101 18-19 -0.5472800535914359 1101 20-21 -0.5889011963097843 1101 22-23 -0.6201755181075796 1101 24-25 -0.596062635208412 1101 26-27 -0.6663538938106868 1101 28-29 -0.7009423302667983 1101 30-31 -0.7195616622553231 1101 32-33 -0.6759858416222926 1101 34-35 -0.7375815357389754 1101 36-37 -0.797262912432025 1101 38-39 -0.8314355842849395 1101 40-41 -0.9482484416730301 1101 42-43 -0.9127618179706474 1101 44-45 -0.8735534386504966 1101 46-47 -0.787186318622517 1101 48-49 -0.7911126782289344 1101 50-51 -0.7741107935088394 1101 52-53 -0.5377672933743369 1101 54-55 -0.5261103808065144 1101 56-57 -0.5763140427923688 1101 58-59 -0.5968496961778698 1101 60-61 -0.6912692657590682 1101 62-63 -0.7142784717283917 1101 64-65 -0.6698026223647062 1101 66-67 -0.7533605662728249 1101 68-69 -0.6710040679860825 1101 70-71 -0.6600174062763742 1101 72-73 -0.8003484172379061 1101 74-75 -0.758869566605874 1101 76-77 -0.7900270297327197 1101 78-79 -0.8853924705000509 1101 80-81 -0.8963866025618685 1101 82-83 -0.909819384691982 1101 84-85 -0.927125271200314 1101 86-87 -0.9011319683929706 1101 88-89 -1.001058704502011 1101 90-91 -1.0778273827758014 1101 92-93 -1.2378383898794993 1101 94-95 -1.2021740855682062 1101 96-97 -1.4326358907991157 1101 98-99 -1.381843544158322 1101 100 -1.3218209518145905 1102 1 -0.22390557193084248 1102 2 -0.24221201112278123 1102 3 -0.2664188853275853 1102 4 -0.16883590951020722 1102 5 -0.17447873729649643 1102 6 -0.16722551042632006 1102 7 -0.2277549150981315 1102 8 -0.17127657846709354 1102 9 -0.2533915587793558 1102 10-11 -0.29000179228020073 1102 12-13 -0.32807083026386863 1102 14-15 -0.4446857998796858 1102 16-17 -0.4954911000970412 1102 18-19 -0.4944262637838648 1102 20-21 -0.5703526057315784 1102 22-23 -0.45685852778624536 1102 24-25 -0.4853877035850118 1102 26-27 -0.5520993535720038 1102 28-29 -0.6522161398294131 1102 30-31 -0.634783580581562 1102 32-33 -0.6051803832301488 1102 34-35 -0.6598314599460835 1102 36-37 -0.669161418415591 1102 38-39 -0.6638621955766126 1102 40-41 -0.8872866706295 1102 42-43 -0.8199322166901624 1102 44-45 -0.6586606081690363 1102 46-47 -0.9932437191056422 1102 48-49 -1.0029812908441258 1102 50-51 -0.8741359926078047 1102 52-53 -0.732127845863765 1102 54-55 -0.7057801519934799 1102 56-57 -0.6942101951214141 1102 58-59 -0.65961220852477 1102 60-61 -0.6128250900760364 1102 62-63 -0.6731854567360642 1102 64-65 -0.7720402893128124 1102 66-67 -0.7669981360756353 1102 68-69 -0.7192370667440571 1102 70-71 -0.8073183317646269 1102 72-73 -0.6321800950806278 1102 74-75 -0.7522443225201698 1102 76-77 -0.77953797813813 1102 78-79 -0.8726844964358875 1102 80-81 -0.8409412500515749 1102 82-83 -0.8768533236078042 1102 84-85 -0.9183168332510583 1102 86-87 -0.8730752329313489 1102 88-89 -0.8983945436189664 1102 90-91 -0.8334568549638846 1102 92-93 -1.1288817890521337 1102 94-95 -1.115454304112525 1102 96-97 -1.179709894000343 1102 98-99 -1.1484024164904945 1102 100 -1.3211107479724618 1103 1 -0.34974122079366055 1103 2 -0.431604570554704 1103 3 -0.4203613315558421 1103 4 -0.2512946159010667 1103 5 -0.3157058392827068 1103 6 -0.28281828597432934 1103 7 -0.322959196254871 1103 8 -0.2578869005829745 1103 9 -0.3567665716434121 1103 10-11 -0.4292137404123437 1103 12-13 -0.4902961827389163 1103 14-15 -0.5777744589308398 1103 16-17 -0.5252567170570046 1103 18-19 -0.686289879097238 1103 20-21 -0.6757870891112177 1103 22-23 -0.6395359873931241 1103 24-25 -0.67675180773238 1103 26-27 -0.7122631129627592 1103 28-29 -0.6745600345500051 1103 30-31 -0.6871767446239545 1103 32-33 -0.7867482539721493 1103 34-35 -0.7475963307909907 1103 36-37 -0.7169554263398439 1103 38-39 -0.721522160071828 1103 40-41 -0.7102223117738902 1103 42-43 -0.8082676082719189 1103 44-45 -0.8486368096715609 1103 46-47 -0.8691454891992905 1103 48-49 -0.915118009996128 1103 50-51 -0.7196964150632894 1103 52-53 -0.6507954516062782 1103 54-55 -0.7002942124030724 1103 56-57 -0.7122769852829833 1103 58-59 -0.536319011035836 1103 60-61 -0.5992795641935587 1103 62-63 -0.6243211596279039 1103 64-65 -0.6322368520379271 1103 66-67 -0.7239706455950383 1103 68-69 -0.6047388934395386 1103 70-71 -0.7303534120363153 1103 72-73 -0.597416665009618 1103 74-75 -0.6875872781649193 1103 76-77 -0.7042198501150736 1103 78-79 -0.7891836731365132 1103 80-81 -0.752915753499142 1103 82-83 -0.7554068252029431 1103 84-85 -0.8465277008027705 1103 86-87 -0.9617011591431464 1103 88-89 -0.9767073587882571 1103 90-91 -0.9572091704933676 1103 92-93 -1.048201344985035 1103 94-95 -1.2039438713657908 1103 96-97 -1.2300756833386188 1103 98-99 -1.2190128801109559 1103 100 -1.1207277851238473 1104 1 -0.3928578895749162 1104 2 -0.33566767283423005 1104 3 -0.35350937425437934 1104 4 -0.21068983663368357 1104 5 -0.23349216687040553 1104 6 -0.19021526030324765 1104 7 -0.18835167356333926 1104 8 -0.33279776069603173 1104 9 -0.34941561474271055 1104 10-11 -0.40122076990175515 1104 12-13 -0.45048047146252657 1104 14-15 -0.5644562146820036 1104 16-17 -0.6489254086919019 1104 18-19 -0.6748335407754951 1104 20-21 -0.48294432849851177 1104 22-23 -0.5667065788832204 1104 24-25 -0.5656330606147648 1104 26-27 -0.5224239146729559 1104 28-29 -0.6013869428132068 1104 30-31 -0.6819173087855432 1104 32-33 -0.6206064578625785 1104 34-35 -0.7444328214032367 1104 36-37 -0.7160102502652919 1104 38-39 -0.6852250010186793 1104 40-41 -0.775667408299725 1104 42-43 -0.7228274923523088 1104 44-45 -0.8552717650940664 1104 46-47 -0.9113353901359531 1104 48-49 -0.8467867862144871 1104 50-51 -0.8101573206399593 1104 52-53 -0.6670214095139215 1104 54-55 -0.6178092624684197 1104 56-57 -0.5273757868159592 1104 58-59 -0.6053381047442414 1104 60-61 -0.6268369361956374 1104 62-63 -0.6027600876030803 1104 64-65 -0.5300142203243681 1104 66-67 -0.5185372850238181 1104 68-69 -0.5276403803965692 1104 70-71 -0.6306534001040021 1104 72-73 -0.5780842474935923 1104 74-75 -0.6562862385939567 1104 76-77 -0.6655183244913232 1104 78-79 -0.5366916078703099 1104 80-81 -0.47122269729746336 1104 82-83 -0.6098616986701195 1104 84-85 -0.5270325734140435 1104 86-87 -0.5692100366300501 1104 88-89 -0.5799683090733261 1104 90-91 -0.5691295094917805 1104 92-93 -0.617541434170235 1104 94-95 -0.6190432581860783 1104 96-97 -0.7179745534949582 1104 98-99 -0.7801342138208831 1104 100 -0.7237707630858274 1105 1 -0.43494754524062884 1105 2 -0.505259798386053 1105 3 -0.5119665499336072 1105 4 -0.3280754345813506 1105 5 -0.26921784533299586 1105 6 -0.2838640885835204 1105 7 -0.31768291529438386 1105 8 -0.3117132382649572 1105 9 -0.4808389388205683 1105 10-11 -0.43611190169046665 1105 12-13 -0.4599856325403948 1105 14-15 -0.5912635400425827 1105 16-17 -0.6541976794317463 1105 18-19 -0.5834319428143857 1105 20-21 -0.7294361721136724 1105 22-23 -0.6288150335266067 1105 24-25 -0.6802378164296741 1105 26-27 -0.6703335185214669 1105 28-29 -0.6380219366108832 1105 30-31 -0.7355229584640028 1105 32-33 -0.872693139583788 1105 34-35 -0.8806791441772717 1105 36-37 -0.8498462138233052 1105 38-39 -0.8009913413915442 1105 40-41 -0.8439018825812212 1105 42-43 -0.8309946124314962 1105 44-45 -0.9443602451035886 1105 46-47 -0.9478258871951368 1105 48-49 -1.0060910200169317 1105 50-51 -0.9432736508356498 1105 52-53 -0.7886257361591191 1105 54-55 -0.6695552967290297 1105 56-57 -0.6244472915790666 1105 58-59 -0.694793557263857 1105 60-61 -0.7711013877897059 1105 62-63 -0.6799289068390948 1105 64-65 -0.7369559624557809 1105 66-67 -0.7735458252131053 1105 68-69 -0.644042164379222 1105 70-71 -0.7077238979504727 1105 72-73 -0.7014031227521045 1105 74-75 -0.7041103685411727 1105 76-77 -0.7313043651462792 1105 78-79 -0.76073725131765 1105 80-81 -0.8296138533289792 1105 82-83 -0.8606813336166326 1105 84-85 -0.7272530269513844 1105 86-87 -0.7942575261312328 1105 88-89 -0.7284474088922472 1105 90-91 -0.7737623941651393 1105 92-93 -0.7559645090992362 1105 94-95 -0.7654837418517069 1105 96-97 -0.8488392728640513 1105 98-99 -0.9715742102300702 1105 100 -0.7724518823066795 1106 1 -0.37647180493875965 1106 2 -0.39523482214905314 1106 3 -0.3439779237908418 1106 4 -0.22315653893604548 1106 5 -0.3183934037407212 1106 6 -0.3330632503561901 1106 7 -0.43374470867577486 1106 8 -0.3915373099571511 1106 9 -0.4507769748564101 1106 10-11 -0.5981914482230763 1106 12-13 -0.4972252999678375 1106 14-15 -0.5652981852212875 1106 16-17 -0.6303365588944843 1106 18-19 -0.6268291523265219 1106 20-21 -0.631778019306175 1106 22-23 -0.5629754994638745 1106 24-25 -0.6639671161645921 1106 26-27 -0.5954547223706115 1106 28-29 -0.6856992229673722 1106 30-31 -0.5961588855676894 1106 32-33 -0.6977031510593577 1106 34-35 -0.6908250220947352 1106 36-37 -0.5718267961217407 1106 38-39 -0.6475737166791191 1106 40-41 -0.6702552618989301 1106 42-43 -0.6768423757447124 1106 44-45 -0.7207903125522037 1106 46-47 -0.6282206757089881 1106 48-49 -0.7345337931454878 1106 50-51 -0.6940495686986736 1106 52-53 -0.5997381547674223 1106 54-55 -0.6217890416503167 1106 56-57 -0.5480807707743551 1106 58-59 -0.5460783613946987 1106 60-61 -0.48253799348443493 1106 62-63 -0.4317443134737715 1106 64-65 -0.4969946046990046 1106 66-67 -0.46512886527177955 1106 68-69 -0.43576327842156104 1106 70-71 -0.480052139570482 1106 72-73 -0.4895134065671769 1106 74-75 -0.5603764518968504 1106 76-77 -0.7414062062543536 1106 78-79 -0.6293518284365689 1106 80-81 -0.7388926667661551 1106 82-83 -0.6567008826760556 1106 84-85 -0.5812550972059434 1106 86-87 -0.627922389820089 1106 88-89 -0.5388724222560874 1106 90-91 -0.5717962110595849 1106 92-93 -0.656105171471232 1106 94-95 -0.57938386609435 1106 96-97 -0.5753292951401505 1106 98-99 -0.594315527840596 1106 100 -0.6731178620812166 1107 1 -0.4788891631169676 1107 2 -0.48194947010074785 1107 3 -0.5338169162699202 1107 4 -0.3213347374520765 1107 5 -0.2802171339209636 1107 6 -0.31544867029504786 1107 7 -0.40375116451210147 1107 8 -0.4376713276466546 1107 9 -0.49829545001236397 1107 10-11 -0.45413212514471013 1107 12-13 -0.5822902395465732 1107 14-15 -0.7260219461525423 1107 16-17 -0.7358005234444391 1107 18-19 -0.6815476890065639 1107 20-21 -0.7659878612402409 1107 22-23 -0.7347394466798249 1107 24-25 -0.7138874691733861 1107 26-27 -0.7012882893432177 1107 28-29 -0.7848462382822987 1107 30-31 -0.7817034118051893 1107 32-33 -0.8763149355039417 1107 34-35 -0.9034882813244209 1107 36-37 -0.9129823279327027 1107 38-39 -0.9508476620557502 1107 40-41 -0.9566959533299269 1107 42-43 -0.9877568767932985 1107 44-45 -0.982603411732093 1107 46-47 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0.8890101470150356 2311 26-27 0.9222638968213843 2311 28-29 0.9722273191107007 2311 30-31 0.9425291517091949 2311 32-33 0.9711242831828883 2311 34-35 0.9639249924184199 2311 36-37 0.9929419454808865 2311 38-39 1.0998723981017022 2311 40-41 1.117501642289639 2311 42-43 1.181362128190834 2311 44-45 1.1710897615826852 2311 46-47 1.1446540875969333 2311 48-49 1.1674491069119242 2311 50-51 1.1529022116932452 2311 52-53 1.0275014794434583 2311 54-55 0.9783612923906801 2311 56-57 0.9597113567327469 2311 58-59 0.9482124357369273 2311 60-61 0.9327172912376014 2311 62-63 0.9354896502499344 2311 64-65 0.9843484126053923 2311 66-67 0.8952255518966865 2311 68-69 0.9462982684637353 2311 70-71 0.9037604653097802 2311 72-73 0.9445051510941056 2311 74-75 0.9375884844187823 2311 76-77 0.8607284006812321 2311 78-79 0.8870026384553995 2311 80-81 0.9063078421713655 2311 82-83 0.9286591398954016 2311 84-85 0.9207526980816034 2311 86-87 1.0149083426634604 2311 88-89 1.0005187127399715 2311 90-91 1.0555524130290124 2311 92-93 1.0872024670659357 2311 94-95 1.0997324176923584 2311 96-97 1.1023281189527836 2311 98-99 1.1156955356365081 2311 100 1.326492427952818 2312 1 0.5817135881833977 2312 2 0.5625993495485595 2312 3 0.5876392402674249 2312 4 0.510681826112652 2312 5 0.521384684303591 2312 6 0.34411374065791733 2312 7 0.5272061229470708 2312 8 0.49266369162211276 2312 9 0.7129831282909151 2312 10-11 0.7565707897867213 2312 12-13 0.826364397690007 2312 14-15 0.9985781012521429 2312 16-17 0.9812237107347386 2312 18-19 0.8988572946238094 2312 20-21 0.9415764517769745 2312 22-23 1.0069565704119796 2312 24-25 1.0834313172534635 2312 26-27 1.0862534019725842 2312 28-29 1.1157821677055821 2312 30-31 1.0026986573508125 2312 32-33 1.1250119142910933 2312 34-35 1.1717928657123977 2312 36-37 1.1398477843628712 2312 38-39 1.209877094293283 2312 40-41 1.2904638053762838 2312 42-43 1.325947380291609 2312 44-45 1.3367372533309165 2312 46-47 1.3592802134073168 2312 48-49 1.3932235326811337 2312 50-51 1.3043325469359388 2312 52-53 1.1660516083162662 2312 54-55 1.150464514978772 2312 56-57 1.1043489177448507 2312 58-59 1.1363369381907873 2312 60-61 1.113069624496788 2312 62-63 1.1501607316916989 2312 64-65 1.1430910970981003 2312 66-67 1.1171166253925762 2312 68-69 1.0639072158976006 2312 70-71 1.0981176542725422 2312 72-73 1.074582536702458 2312 74-75 1.136157399915568 2312 76-77 1.1217731974198486 2312 78-79 1.2013968124037149 2312 80-81 0.8758286981166847 2312 82-83 1.029321176586123 2312 84-85 1.0651532048294037 2312 86-87 1.156508085710442 2312 88-89 1.1555150946166357 2312 90-91 1.2119429458188833 2312 92-93 1.322627105204063 2312 94-95 1.2751410557125844 2312 96-97 1.29331917761208 2312 98-99 1.0866935339343833 2312 100 1.1807922173672658 2313 1 0.4603783954155567 2313 2 0.5291563774594756 2313 3 0.5553995368557132 2313 4 0.44074578508388385 2313 5 0.42880887658899525 2313 6 0.40995751058259344 2313 7 0.48563151889510436 2313 8 0.5102765607513788 2313 9 0.5842420466673133 2313 10-11 0.5124303721776116 2313 12-13 0.6383606291229356 2313 14-15 0.8338339916836972 2313 16-17 0.8573891149496191 2313 18-19 0.8268216017238359 2313 20-21 0.862486847397733 2313 22-23 0.8910610720859538 2313 24-25 0.973891912151295 2313 26-27 0.9850904978122514 2313 28-29 0.9513620955100635 2313 30-31 0.9628664397994058 2313 32-33 1.1026810482843246 2313 34-35 1.0621795347548684 2313 36-37 1.0802156954203284 2313 38-39 1.0875063031729937 2313 40-41 1.0950838001155603 2313 42-43 1.1274585799299075 2313 44-45 1.1470544329784644 2313 46-47 1.0926025313488026 2313 48-49 1.1211810517542773 2313 50-51 1.0819357704002073 2313 52-53 0.9679016789844752 2313 54-55 0.9990647874695711 2313 56-57 0.9007486573755301 2313 58-59 0.8574804752618945 2313 60-61 0.9540123359741344 2313 62-63 0.9796539963932389 2313 64-65 0.9191811360322859 2313 66-67 0.9324065195963342 2313 68-69 0.9677723305304013 2313 70-71 0.9584358187900719 2313 72-73 0.9892757663729199 2313 74-75 0.9599553888413865 2313 76-77 0.9552113360701497 2313 78-79 0.9643000030187849 2313 80-81 1.0037784291411143 2313 82-83 0.9839313983849038 2313 84-85 1.033736686044751 2313 86-87 1.0229499292900641 2313 88-89 1.057597458231843 2313 90-91 1.0821151889708531 2313 92-93 1.1428635921819534 2313 94-95 1.174535890993841 2313 96-97 1.1185102423868258 2313 98-99 1.1823684244168433 2313 100 1.3149494474644072 2314 1 0.40722846478191954 2314 2 0.415595543918176 2314 3 0.46629510984156397 2314 4 0.31411446798165343 2314 5 0.3321566198788872 2314 6 0.3354825437451723 2314 7 0.39076668714990603 2314 8 0.37935052202249864 2314 9 0.5934137022559369 2314 10-11 0.5714646367465548 2314 12-13 0.3078349116886727 2314 14-15 0.6228053211475384 2314 16-17 0.6819950773263059 2314 18-19 0.666597062220788 2314 20-21 0.721679388925871 2314 22-23 0.5850576390994107 2314 24-25 0.5446226606371738 2314 26-27 0.6740397785219656 2314 28-29 0.8230840540119004 2314 30-31 0.7576502770339815 2314 32-33 0.8299044169225027 2314 34-35 0.8462555041173871 2314 36-37 0.9083272308096966 2314 38-39 0.9084214024997195 2314 40-41 0.9488974742788088 2314 42-43 1.0524963937668588 2314 44-45 1.070736315521664 2314 46-47 1.1092890105876378 2314 48-49 1.1523703338235904 2314 50-51 1.0808088688628743 2314 52-53 0.8624534005315212 2314 54-55 0.8304242877097323 2314 56-57 0.8048887246577721 2314 58-59 0.7783623449246946 2314 60-61 0.7802146192053598 2314 62-63 0.8344272162358664 2314 64-65 0.8250718654480025 2314 66-67 0.8422796207424241 2314 68-69 0.8854690273318582 2314 70-71 0.845311622138432 2314 72-73 0.8521236156578382 2314 74-75 0.872647286796358 2314 76-77 0.8826669992904144 2314 78-79 0.9662323772983967 2314 80-81 1.0092122618586075 2314 82-83 0.7738669247866099 2314 84-85 0.8221140408561602 2314 86-87 1.0237723522916156 2314 88-89 1.1626303435237624 2314 90-91 1.202370569895507 2314 92-93 1.2442286918217604 2314 94-95 1.2279832785519034 2314 96-97 1.2205940509088258 2314 98-99 1.2762809109885715 2314 100 1.2164086903647515 2315 1 0.33800402589555034 2315 2 0.361407136004253 2315 3 0.40490680774918886 2315 4 0.26844730385666793 2315 5 0.2470181292948368 2315 6 -0.4300521139075002 2315 7 0.11989016489286541 2315 8 0.10891975412232568 2315 9 0.32116424474816085 2315 10-11 0.3909906031653705 2315 12-13 0.39434271410550537 2315 14-15 0.5079235087594256 2315 16-17 0.40883580175197665 2315 18-19 0.5171930538390654 2315 20-21 0.5192177008768937 2315 22-23 0.4680992089025011 2315 24-25 0.5597251406582373 2315 26-27 0.616558778098927 2315 28-29 0.7285630701625223 2315 30-31 0.7538131069890781 2315 32-33 0.8180409619825255 2315 34-35 0.76016386234582 2315 36-37 0.7854083963777683 2315 38-39 0.8057242425672584 2315 40-41 0.869460158256004 2315 42-43 0.9264561992061786 2315 44-45 0.9228697455662456 2315 46-47 0.9178237601124408 2315 48-49 0.9019352426681522 2315 50-51 0.8008848494233689 2315 52-53 0.6905805034080146 2315 54-55 0.5112489350446836 2315 56-57 0.5377586391019236 2315 58-59 0.4783456002968549 2315 60-61 0.39422076239254267 2315 62-63 0.6565713461597369 2315 64-65 0.6724386817662733 2315 66-67 0.6488038613494425 2315 68-69 0.7503411526229726 2315 70-71 0.7001396180440054 2315 72-73 0.7603695181341195 2315 74-75 0.6931102543111862 2315 76-77 0.6950418915417984 2315 78-79 0.714573295554743 2315 80-81 0.6932776054837504 2315 82-83 0.7803277452266428 2315 84-85 0.7394872888152655 2315 86-87 0.7978989325677048 2315 88-89 0.9557688351726519 2315 90-91 1.0237298970673123 2315 92-93 1.0963544461360328 2315 94-95 1.138998152667856 2315 96-97 1.1128607689008376 2315 98-99 1.255689485172713 2315 100 1.1971045096621467 2316 1 -0.14473711650054355 2316 2 -0.1578224816623255 2316 3 -0.09074772906076234 2316 4 -0.13363908832724292 2316 5 -0.08488914865137076 2316 6 -0.02784613456723406 2316 7 -0.02243472819466774 2316 8 -0.00969724849756659 2316 9 0.05667536707040455 2316 10-11 -0.05408229629339445 2316 12-13 0.0442227042401413 2316 14-15 0.21925731422020078 2316 16-17 0.2711897122768505 2316 18-19 0.23235486231163804 2316 20-21 0.21130462927229843 2316 22-23 0.10524177735788243 2316 24-25 0.1349615073933137 2316 26-27 0.20970577876791907 2316 28-29 0.24887770432192013 2316 30-31 0.25918886852713996 2316 32-33 0.36586551276352 2316 34-35 0.31945587946726306 2316 36-37 0.3463140210198361 2316 38-39 0.43421878448716456 2316 40-41 0.4271158966126478 2316 42-43 0.4213078521870699 2316 44-45 0.3457630596934749 2316 46-47 0.38501931813995327 2316 48-49 0.4263313736024017 2316 50-51 0.16585253500205965 2316 52-53 -0.03715755613766447 2316 54-55 -0.07659597831650444 2316 56-57 0.010045032321407632 2316 58-59 0.11324184337331644 2316 60-61 0.16123572860229984 2316 62-63 0.18612043149497737 2316 64-65 0.22581623933492523 2316 66-67 0.150765669927317 2316 68-69 0.14244495720000572 2316 70-71 0.04719930247842541 2316 72-73 0.1678578388598524 2316 74-75 0.24294919632993128 2316 76-77 0.3038390860879545 2316 78-79 0.3640656220406626 2316 80-81 0.4289917597675057 2316 82-83 0.3952334505490853 2316 84-85 0.4123366426687163 2316 86-87 0.46171462933563134 2316 88-89 0.5627902862358347 2316 90-91 0.5889862065988929 2316 92-93 0.6027592773869443 2316 94-95 0.6862386775723941 2316 96-97 0.7729742807488158 2316 98-99 0.8343992912873581 2316 100 0.7184159033547957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 25.0 1 209.0 2 122.0 3 108.0 4 83.0 5 59.0 6 125.0 7 850.0 8 3028.0 9 4893.0 10 5892.0 11 6858.0 12 7416.0 13 7961.0 14 8340.0 15 9266.0 16 10935.0 17 13406.0 18 16778.0 19 20071.0 20 24470.0 21 29333.0 22 33052.0 23 34188.0 24 34810.0 25 36194.0 26 40890.0 27 48888.0 28 59570.0 29 72789.0 30 91361.0 31 117224.0 32 154923.0 33 209957.0 34 302317.0 35 420087.0 36 630430.0 37 962337.0 38 978308.0 39 260566.0 40 214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26007641207448 19.984145355040788 13.459883660895605 23.295894571989123 2 15.366921593951913 21.34929308841511 40.853530602452736 22.43025471518024 3 17.599728596075163 28.581796397671166 30.877435576459572 22.941039429794095 4 12.1688061669828 18.124701813361252 40.27511112618893 29.43138089346701 5 12.815638509036157 38.155874039202345 34.49421395607708 14.53427349568441 6 27.62220369365666 38.035178787054804 20.896656567596118 13.44596095169242 7 25.895656570668447 33.39476463541703 24.06098710651479 16.64859168739973 8 26.019749573551653 34.40414716989075 21.72349995639939 17.852603300158208 9 27.376473460899238 16.196349637461346 21.281533281978255 35.145643619661165 10-11 24.313696013297957 26.130761078139447 29.75115728933898 19.80438561922362 12-13 25.242460529831025 24.330957119921106 28.922824534170825 21.503757816077044 14-15 22.992618841546538 25.68246783236738 26.960477296448083 24.364436029638 16-17 22.1189461973552 27.854134713107992 27.58435336911595 22.44256572042086 18-19 21.78098999259152 27.581301941095216 29.05891884690487 21.5787892194084 20-21 23.028471193905816 27.025609914121883 28.315826800031306 21.630092091941 22-23 22.69912778494824 26.93912230528794 28.158164210732444 22.203585699031375 24-25 22.172285408163045 26.938224261540412 28.473707038152764 22.415783292143782 26-27 22.132683593790258 26.97820441030468 28.289961300761618 22.599150695143443 28-29 22.090342142690282 27.584363362785645 28.260577841369738 22.064716653154328 30-31 23.06175253212091 26.849799830726745 28.3132813375593 21.77516629959305 32-33 22.94353606229822 27.035587711659648 28.001627153931057 22.01924907211107 34-35 22.960970836158246 27.16550476169004 28.1589509627251 21.71457343942661 36-37 22.660033736516773 27.255420989301022 28.04510068993238 22.03944458424982 38-39 22.631978488064437 27.32742414282544 28.03807939821204 22.00251797089808 40-41 22.741605164229327 26.720643948340527 28.39973993336866 22.13801095406148 42-43 22.47353289355159 26.95096872304465 27.899603758379577 22.675894625024185 44-45 22.115333096178418 27.244826445443294 28.253257399378366 22.386583058999925 46-47 22.11061195808203 26.723223344039905 27.902230768768206 23.263933929109864 48-49 22.27493011622617 26.707794286308236 28.184907424200013 22.83236817326559 50-51 22.414068549327403 27.061744984889497 27.839741500262182 22.684444965520914 52-53 22.098983640531962 27.293133533419596 27.67194085464395 22.93594197140449 54-55 21.9932035310198 26.936283344914198 28.09328517154443 22.977227952521574 56-57 22.403223073422442 26.900333548024495 27.72576343324884 22.970679945304223 58-59 22.135042188938794 27.00043759446537 28.129238021858242 22.73528219473759 60-61 22.463623801466138 27.1887800407133 27.780669953977714 22.566926203842847 62-63 22.449532615661344 26.91706919362446 27.818566388846033 22.814831801868163 64-65 22.0043002957943 26.622142024853762 27.97867738687943 23.394880292472507 66-67 22.115799842082534 26.598882123008554 27.750666059963542 23.534651974945366 68-69 21.75002789021253 26.842442471806187 27.975456183482915 23.43207345449837 70-71 21.997798775904652 26.425093954686997 28.200697949103404 23.37640932030495 72-73 22.050609847633122 26.837032708398702 27.745388156802562 23.366969287165613 74-75 22.251962483252985 26.86362232371201 27.574665191236704 23.309750001798303 76-77 21.99794459289767 26.60432812095576 27.791667646275453 23.60605963987112 78-79 21.70484689797027 26.586216040098638 28.09448815516429 23.614448906766803 80-81 21.90178320960982 26.80381945954152 27.755871592933378 23.538525737915283 82-83 22.369100218189566 26.618439659055724 27.90813493656786 23.104325186186852 84-85 22.491492449881374 26.83885834318514 27.325292368705266 23.344356838228215 86-87 22.009539844318947 27.01332065762535 27.686535151902387 23.29060434615332 88-89 22.131394368291353 26.394097221290323 27.789716192329845 23.68479221808848 90-91 22.65557642901519 26.263693309796743 27.819154321069167 23.2615759401189 92-93 22.531761214924177 26.54962736800067 27.614414449232516 23.304196967842643 94-95 22.20351300303259 26.403371266740468 27.64114286131455 23.751972868912397 96-97 22.20125708512782 26.302603333224184 27.725431737185573 23.77070784446242 98-99 22.198268781440905 26.494974931022462 27.430834037119993 23.875922250416632 100 22.387186351990092 26.287360112966105 27.406349018203247 23.919104516840555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 93.0 1 114.5 2 361.5 3 560.5 4 416.0 5 363.5 6 522.5 7 802.0 8 1224.0 9 1579.5 10 1612.5 11 1598.5 12 1833.5 13 2091.5 14 2350.0 15 2734.5 16 3093.5 17 3474.5 18 3883.0 19 4116.0 20 4479.5 21 5148.5 22 6068.0 23 7387.0 24 9158.0 25 11687.0 26 15252.0 27 19595.0 28 25422.5 29 33760.0 30 44604.0 31 56333.0 32 70448.0 33 85237.5 34 98686.5 35 111939.0 36 127137.5 37 146108.5 38 156385.5 39 156071.0 40 155354.0 41 156095.0 42 158433.0 43 162708.5 44 168569.0 45 176840.5 46 183539.0 47 187609.5 48 192485.0 49 194355.5 50 192727.0 51 189085.0 52 181139.0 53 170606.0 54 158921.0 55 143880.0 56 128565.0 57 112296.0 58 94344.5 59 77472.5 60 60600.5 61 46248.5 62 36017.0 63 27531.5 64 19948.5 65 14268.0 66 10617.0 67 8091.0 68 6120.5 69 4648.5 70 3650.5 71 2794.0 72 2186.0 73 1750.5 74 1339.5 75 978.5 76 746.5 77 536.0 78 375.0 79 276.5 80 194.5 81 161.5 82 124.5 83 82.0 84 70.5 85 56.0 86 39.5 87 36.5 88 32.0 89 20.5 90 17.0 91 19.5 92 14.0 93 8.0 94 5.5 95 6.0 96 4.0 97 1.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21151343195087172 2 0.14058677213501053 3 0.02292665638115609 4 0.021788910324787858 5 0.02715563700577009 6 0.052787123634141224 7 0.05851878772943025 8 0.05227191787276693 9 0.04001431413340352 10-11 0.042461541499931414 12-13 0.0408944573090846 14-15 0.020050090880149616 16-17 0.01840787251576905 18-19 0.01789266675439476 20-21 0.020962434415916595 22-23 0.023828266463561107 24-25 0.02153130744410071 26-27 0.01882647719688567 28-29 0.018483006689302806 30-31 0.016636852711044918 32-33 0.02407513589088629 34-35 0.023838999916923072 36-37 0.022862255660984303 38-39 0.021713776151254107 40-41 0.022862255660984303 42-43 0.028465118315929752 44-45 0.03740608496644615 46-47 0.04295528035458178 48-49 0.039745977799354404 50-51 0.028057247088175107 52-53 0.04310554870164928 54-55 0.04174240012467979 56-57 0.043620754463023576 58-59 0.047130593712385954 60-61 0.024311271864849507 62-63 0.025577819361561314 64-65 0.026007157496039893 66-67 0.03155635288417552 68-69 0.03609660365628649 70-71 0.039348840024961716 72-73 0.04306261488820142 74-75 0.024890878346395588 76-77 0.018493740142664767 78-79 0.017559929700173862 80-81 0.02087656678902088 82-83 0.019642219652394967 84-85 0.011989267405314304 86-87 0.012987478567977 88-89 0.018579607769560483 90-91 0.019395350225069784 92-93 0.019813954906186395 94-95 0.02345259559589235 96-97 0.02426833805140165 98-99 0.029667265092469777 100 0.029216460051267264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4658333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.60413295610466 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84930658830571 55.46596578592994 2 12.478340480240607 17.121314587559393 3 3.272759909820818 6.735745679602696 4 1.2757923301318663 3.500985065629806 5 0.6296147698400242 2.159708769061613 6 0.3658455593668182 1.5059110437721008 7 0.2466830577314777 1.1846434103440118 8 0.1653413671355799 0.9074480907290751 9 0.1283443590175206 0.7924458123183611 >10 0.5462778724375621 6.393319640054732 >50 0.026723687444800307 1.2417236857832998 >100 0.013745237832011646 1.793456825986641 >500 8.645511676615726E-4 0.39365722045885887 >1k 2.8818362203705215E-4 0.3906420962003383 >5k 3.602295275462878E-5 0.1650596925742995 >10k+ 3.602295275462878E-5 0.24797259399468802 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10034 0.21539894206790283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6679 0.1433774700091213 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.293381344785785E-5 0.0 5 0.0 0.0 0.0 6.440072017178677E-5 0.0 6 0.0 0.0 0.0 6.440072017178677E-5 2.1466906723928925E-5 7 0.0 0.0 0.0 6.440072017178677E-5 2.1466906723928925E-5 8 0.0 0.0 0.0 6.440072017178677E-5 6.440072017178677E-5 9 0.0 0.0 0.0 2.576028806871471E-4 6.440072017178677E-5 10-11 0.0 0.0 0.0 4.293381344785785E-4 6.440072017178677E-5 12-13 0.0 0.0 0.0 4.7227194792643637E-4 7.513417353375123E-5 14-15 1.0733453361964463E-5 1.0733453361964463E-5 0.0 5.152057613742943E-4 8.58676268957157E-5 16-17 2.1466906723928925E-5 2.1466906723928925E-5 0.0 6.118068416319743E-4 8.58676268957157E-5 18-19 2.1466906723928925E-5 2.1466906723928925E-5 0.0 7.084079218896545E-4 1.2880144034357354E-4 20-21 2.1466906723928925E-5 3.220036008589339E-5 0.0 8.157424555092991E-4 1.717352537914314E-4 22-23 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.0015241503773989536 2.254025206012537E-4 24-25 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.0026618964337671866 2.3613597396321818E-4 26-27 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.00447585005193918 2.3613597396321818E-4 28-29 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.008060823474835312 2.3613597396321818E-4 30-31 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.02051162937471409 2.3613597396321818E-4 32-33 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.05231485168621479 2.576028806871471E-4 34-35 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.10216100909917775 2.79069787411076E-4 36-37 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.18020394849402135 2.79069787411076E-4 38-39 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.32428982642503235 2.79069787411076E-4 40-41 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.5156350995087728 2.79069787411076E-4 42-43 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.7078068485013844 2.79069787411076E-4 44-45 2.1466906723928925E-5 4.293381344785785E-5 0.0 0.9341861133585769 2.79069787411076E-4 46-47 2.1466906723928925E-5 4.293381344785785E-5 0.0 1.1872487432736132 2.898032407730405E-4 48-49 2.1466906723928925E-5 4.293381344785785E-5 0.0 1.4695815005067263 3.0053669413500497E-4 50-51 2.1466906723928925E-5 4.293381344785785E-5 0.0 1.7885046002507763 3.2200360085893386E-4 52-53 2.1466906723928925E-5 4.293381344785785E-5 0.0 2.134690671534216 3.2200360085893386E-4 54-55 2.1466906723928925E-5 4.293381344785785E-5 0.0 2.513205904343893 3.3273705422089833E-4 56-57 2.1466906723928925E-5 4.293381344785785E-5 0.0 2.908991263612971 3.434705075828628E-4 58-59 2.1466906723928925E-5 4.293381344785785E-5 0.0 3.3660217077654173 3.434705075828628E-4 60-61 2.1466906723928925E-5 4.293381344785785E-5 0.0 3.839731938442357 3.434705075828628E-4 62-63 2.1466906723928925E-5 4.293381344785785E-5 0.0 4.343860775947103 3.434705075828628E-4 64-65 2.1466906723928925E-5 4.293381344785785E-5 0.0 4.811452938207724 3.434705075828628E-4 66-67 2.1466906723928925E-5 4.293381344785785E-5 0.0 5.325510220072287 3.434705075828628E-4 68-69 2.1466906723928925E-5 4.293381344785785E-5 0.0 5.89326696910676 3.434705075828628E-4 70-71 2.1466906723928925E-5 4.293381344785785E-5 0.0 6.4965299818626105 3.434705075828628E-4 72-73 2.1466906723928925E-5 4.293381344785785E-5 0.0 7.080215175686238 3.434705075828628E-4 74-75 2.1466906723928925E-5 4.293381344785785E-5 0.0 7.647735788746747 3.434705075828628E-4 76-77 2.1466906723928925E-5 4.293381344785785E-5 0.0 8.241488961823897 3.6493741430679175E-4 78-79 2.1466906723928925E-5 4.293381344785785E-5 0.0 8.900050726300588 3.6493741430679175E-4 80-81 2.1466906723928925E-5 4.293381344785785E-5 0.0 9.538358464283252 3.864043210307207E-4 82-83 2.1466906723928925E-5 4.293381344785785E-5 0.0 10.206236866278129 4.078712277546496E-4 84-85 2.1466906723928925E-5 4.293381344785785E-5 0.0 10.86731025884152 4.078712277546496E-4 86-87 2.1466906723928925E-5 4.293381344785785E-5 0.0 11.53351424211193 4.18604681116614E-4 88 2.1466906723928925E-5 4.293381344785785E-5 0.0 12.02644808776015 4.293381344785785E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4965 0.0 42.28261 2 GGTATCA 7120 0.0 41.16593 1 GTATCAA 12460 0.0 40.957924 1 ACCTGGG 5695 0.0 36.31877 3 CAACGCA 15090 0.0 33.30126 5 ATCAACG 15125 0.0 33.224197 3 TCAACGC 15630 0.0 32.18081 4 TATCAAC 15800 0.0 32.055416 2 GTACCTG 6750 0.0 31.484753 1 AACGCAG 16190 0.0 31.473856 6 CCTGGGG 5240 0.0 28.34836 4 ACGCAGA 18095 0.0 27.926558 7 GTACATG 18285 0.0 27.858532 1 CGCAGAG 18530 0.0 27.24443 8 TACATGG 18265 0.0 27.13657 2 TATCACG 540 0.0 27.021793 2 ACATGGG 18470 0.0 25.807339 3 TAACGCA 575 0.0 25.343466 4 CATGGGG 13945 0.0 24.77656 4 GCAGAGT 20425 0.0 24.187418 9 >>END_MODULE