FastQCFastQC Report
Mon 6 Jun 2016
ERR1211379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211379_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658333
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85600.1837567215568316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65900.1414669153106916No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT51670.11091950704254076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45050.044.854472
GGTATCA65100.044.631361
GTATCAA119600.040.331911
ACCTGGG55350.035.4887543
CAACGCA144700.032.40735
ATCAACG144750.032.3025323
GTACCTG64800.031.4881631
TCAACGC151100.031.0386494
TATCAAC152450.030.9484122
AACGCAG157650.030.280426
TAACGCA6800.029.0253264
CCTGGGG51550.027.7128814
ATAACGC7300.027.680743
ACGCAGA175050.026.9216067
CGCAGAG178950.026.334888
GTACATG164650.025.7273861
TACATGG166000.024.9401992
TGGGGGG94800.024.0378486
ACATGGG167300.023.5947533
ATGGGGG95400.023.1981495
Produced by FastQC (version 0.11.5)