##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211379_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4658333 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81456735703523 31.0 31.0 34.0 30.0 34.0 2 31.971402001531448 31.0 31.0 34.0 30.0 34.0 3 32.038164725450066 33.0 31.0 34.0 30.0 34.0 4 35.7457380140063 37.0 35.0 37.0 35.0 37.0 5 35.58522844974801 37.0 35.0 37.0 33.0 37.0 6 35.61617406913589 37.0 35.0 37.0 33.0 37.0 7 35.54576476177208 37.0 35.0 37.0 33.0 37.0 8 35.52294136121226 37.0 35.0 37.0 33.0 37.0 9 37.053835782027605 39.0 37.0 39.0 33.0 39.0 10-11 37.016604008343755 39.0 37.0 39.0 33.0 39.0 12-13 36.93120113997861 39.0 37.0 39.0 33.0 39.0 14-15 38.056352562171924 40.0 37.0 41.0 33.0 41.0 16-17 38.03926168438366 40.0 37.0 41.0 33.0 41.0 18-19 38.03747692575864 40.0 37.0 41.0 33.0 41.0 20-21 37.99365738773935 40.0 37.0 41.0 33.0 41.0 22-23 37.88942986686439 40.0 37.0 41.0 33.0 41.0 24-25 37.76116252316011 39.5 37.0 41.0 32.5 41.0 26-27 37.62022283507856 39.0 37.0 41.0 32.0 41.0 28-29 37.53764694365989 39.0 36.0 41.0 32.0 41.0 30-31 37.59202540908947 39.0 37.0 41.0 32.0 41.0 32-33 37.440768618301874 39.0 36.0 41.0 32.0 41.0 34-35 37.329152402801604 39.0 36.0 41.0 31.5 41.0 36-37 37.18780183812535 39.0 36.0 41.0 31.0 41.0 38-39 37.072514566906236 39.0 36.0 41.0 31.0 41.0 40-41 37.0239551358823 39.0 36.0 40.5 31.0 41.0 42-43 36.90778557479682 39.0 35.5 40.0 31.0 41.0 44-45 36.77459876741315 39.0 35.0 40.0 30.0 41.0 46-47 36.58324340488325 39.0 35.0 40.0 30.0 41.0 48-49 36.39497509087478 39.0 35.0 40.0 30.0 41.0 50-51 36.22318584781294 39.0 35.0 40.0 29.0 41.0 52-53 36.02261849893513 38.0 35.0 40.0 28.5 41.0 54-55 35.83115472852627 38.0 34.0 40.0 28.0 41.0 56-57 35.60034866549901 38.0 34.0 40.0 28.0 41.0 58-59 35.33480152664054 38.0 34.0 40.0 27.0 41.0 60-61 35.08072705407707 37.0 34.0 40.0 27.0 41.0 62-63 34.790037659394464 37.0 33.0 40.0 26.0 41.0 64-65 34.440670299869076 36.5 33.0 39.0 26.0 41.0 66-67 34.09083077573029 36.0 32.5 39.0 26.0 40.0 68-69 33.73630320116659 35.5 32.0 39.0 25.5 40.0 70-71 33.36743111752638 35.0 32.0 38.0 25.0 40.0 72-73 33.002345366894126 35.0 31.5 37.5 24.5 39.5 74-75 32.625793175369814 35.0 31.0 37.0 24.0 39.0 76-77 31.685233215401304 34.0 30.0 36.0 23.0 38.5 78-79 32.21853128576252 34.0 31.0 36.0 24.5 38.5 80-81 32.12045660969278 34.0 31.0 36.0 24.5 37.5 82-83 31.835040131308773 34.0 31.0 35.0 24.0 37.0 84-85 31.535895244071213 34.0 31.0 35.0 23.5 36.5 86-87 31.267612040616246 34.0 30.5 35.0 23.0 36.0 88-89 31.02382730045276 34.0 30.0 35.0 22.5 36.0 90-91 30.789967355274946 34.0 30.0 35.0 20.5 35.0 92-93 30.58703156257829 34.0 29.5 35.0 20.0 35.0 94-95 30.39699845416805 34.0 29.0 35.0 20.0 35.0 96-97 30.198466597385803 33.5 29.0 35.0 19.5 35.0 98-99 29.984108048952276 33.0 29.0 35.0 19.0 35.0 100 28.162382122531817 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.48177958553470646 1101 2 -0.4465113617398586 1101 3 -0.49517594920249763 1101 4 -0.38156662587010004 1101 5 -0.37355047427236343 1101 6 -0.29785762896361945 1101 7 -0.33392616257040686 1101 8 -0.3640469442998153 1101 9 -0.6165107501163121 1101 10-11 -0.5572959858977171 1101 12-13 -0.5771437830209081 1101 14-15 -0.7934376167851909 1101 16-17 -0.8182172850666731 1101 18-19 -0.8681682238691906 1101 20-21 -0.8070940913164293 1101 22-23 -0.9048199274841693 1101 24-25 -0.9332874699673042 1101 26-27 -0.8643950599583832 1101 28-29 -1.025593179743062 1101 30-31 -0.8662408072768173 1101 32-33 -0.827575001857106 1101 34-35 -0.898742414172709 1101 36-37 -0.9333461859440249 1101 38-39 -1.0159127739126887 1101 40-41 -1.1113071401866534 1101 42-43 -1.0472027969617628 1101 44-45 -1.1770968463854885 1101 46-47 -1.255764112379424 1101 48-49 -1.0915688689409748 1101 50-51 -1.2334461968766774 1101 52-53 -1.2577786379122315 1101 54-55 -1.1688983660060401 1101 56-57 -1.255450823114444 1101 58-59 -1.315981642072508 1101 60-61 -1.2001517141175668 1101 62-63 -1.2259988671105333 1101 64-65 -1.3650111365057498 1101 66-67 -1.3187766959709037 1101 68-69 -1.3078758355180682 1101 70-71 -1.4663772991399604 1101 72-73 -1.6805633639610704 1101 74-75 -1.578005086421463 1101 76-77 -1.276940135177501 1101 78-79 -1.2420712000485992 1101 80-81 -1.0801691519856504 1101 82-83 -1.2333756781411402 1101 84-85 -1.2682158914262338 1101 86-87 -1.346244288023378 1101 88-89 -1.3706236946956452 1101 90-91 -1.4309288163614013 1101 92-93 -1.571090205889334 1101 94-95 -1.4106894702493449 1101 96-97 -1.469949238501627 1101 98-99 -1.5984967534331709 1101 100 -1.4391450759681952 1102 1 -0.40517452028762335 1102 2 -0.3792278767664108 1102 3 -0.4340035554184922 1102 4 -0.292980611533018 1102 5 -0.29789991047104536 1102 6 -0.35672161576750483 1102 7 -0.2926926780859418 1102 8 -0.3476752233610938 1102 9 -0.652272411626889 1102 10-11 -0.6418598263316753 1102 12-13 -0.6120270293689956 1102 14-15 -0.7769094201996154 1102 16-17 -0.878689743722866 1102 18-19 -0.783867197323751 1102 20-21 -0.7918460285175328 1102 22-23 -0.8866795753487651 1102 24-25 -0.9075281881905184 1102 26-27 -1.0382223890820939 1102 28-29 -0.8497991147339619 1102 30-31 -0.7793452841102706 1102 32-33 -0.8636265345034673 1102 34-35 -0.8672685439757828 1102 36-37 -0.8517149925238314 1102 38-39 -1.0455752429027996 1102 40-41 -0.9982200712689107 1102 42-43 -1.0844990607796987 1102 44-45 -1.0173817950582205 1102 46-47 -1.0816544751095307 1102 48-49 -1.1680064726468373 1102 50-51 -1.1811477000208654 1102 52-53 -1.1730655914291859 1102 54-55 -1.198057863588815 1102 56-57 -1.1828885669964109 1102 58-59 -1.320248243975648 1102 60-61 -1.2736287659859826 1102 62-63 -1.4834349810378384 1102 64-65 -1.3932766365595413 1102 66-67 -1.2325401960703246 1102 68-69 -1.3332520127528227 1102 70-71 -1.367473508843407 1102 72-73 -1.4539237762743973 1102 74-75 -1.4458052670204395 1102 76-77 -1.190053022576972 1102 78-79 -1.0451590513445765 1102 80-81 -0.9745259881784705 1102 82-83 -0.9914521360540824 1102 84-85 -1.096711280284513 1102 86-87 -1.2047918680297016 1102 88-89 -1.126937226253201 1102 90-91 -1.183997531989732 1102 92-93 -1.2024935642479022 1102 94-95 -1.3172450826722084 1102 96-97 -1.327455212285436 1102 98-99 -1.3117291817070154 1102 100 -1.1162360632969275 1103 1 -0.4250651758397481 1103 2 -0.3908491333270767 1103 3 -0.42119584727812054 1103 4 -0.2287920759767772 1103 5 -0.2254440341717796 1103 6 -0.36088159312677703 1103 7 -0.39699030080900855 1103 8 -0.529752639230189 1103 9 -0.6933927419757566 1103 10-11 -0.5786391177796588 1103 12-13 -0.7588716058843801 1103 14-15 -0.9741776742903809 1103 16-17 -0.9996819481069394 1103 18-19 -0.9178079069048906 1103 20-21 -0.8192847313064604 1103 22-23 -0.9718848213094589 1103 24-25 -0.9292709457288524 1103 26-27 -1.051139505990605 1103 28-29 -1.0816335286477141 1103 30-31 -0.9853116960686918 1103 32-33 -0.9287796810507558 1103 34-35 -0.9839241475571399 1103 36-37 -0.9482564276375527 1103 38-39 -1.0583922131611132 1103 40-41 -1.056884975560358 1103 42-43 -1.162965344097593 1103 44-45 -1.2212137390738604 1103 46-47 -1.2342999675454678 1103 48-49 -1.1709062230842164 1103 50-51 -1.1195371206239244 1103 52-53 -1.1471990045196492 1103 54-55 -1.289568039054494 1103 56-57 -1.2369309669140733 1103 58-59 -1.5201957586406607 1103 60-61 -1.4000930167835506 1103 62-63 -1.3216989128069088 1103 64-65 -1.4779160571626022 1103 66-67 -1.6370818498729491 1103 68-69 -1.3880466381516072 1103 70-71 -1.3766505953927535 1103 72-73 -1.5122566593658853 1103 74-75 -1.4829960151937414 1103 76-77 -1.2365956797757 1103 78-79 -1.0319269581059238 1103 80-81 -1.0650109629649265 1103 82-83 -1.0855754508631783 1103 84-85 -1.194330401412433 1103 86-87 -1.3305583726093033 1103 88-89 -1.1789988836577443 1103 90-91 -1.364259251141732 1103 92-93 -1.3587763675822657 1103 94-95 -1.3318626343857005 1103 96-97 -1.4443128458143057 1103 98-99 -1.5383353230057715 1103 100 -1.504007494808711 1104 1 -0.40053534406013114 1104 2 -0.3678599917283307 1104 3 -0.45408156797951804 1104 4 -0.3413051914472973 1104 5 -0.33897020016479473 1104 6 -0.35704877937752855 1104 7 -0.4570324717038403 1104 8 -0.4934140380593135 1104 9 -0.5474400226176286 1104 10-11 -0.5505401347446792 1104 12-13 -0.6368636827304641 1104 14-15 -0.8739499597655893 1104 16-17 -0.7686344952773183 1104 18-19 -0.8843734950821371 1104 20-21 -0.8481309311429399 1104 22-23 -0.8506113707978287 1104 24-25 -0.9453406382176368 1104 26-27 -0.9572882764592947 1104 28-29 -0.8836375020562883 1104 30-31 -0.9473892633779926 1104 32-33 -0.8866984358715158 1104 34-35 -1.042025052600117 1104 36-37 -0.8046790622209059 1104 38-39 -0.9755720446386391 1104 40-41 -1.0645221253697486 1104 42-43 -0.9789509817763005 1104 44-45 -0.9370165971891424 1104 46-47 -0.9603090406316213 1104 48-49 -0.9901875947358647 1104 50-51 -1.0355115119754714 1104 52-53 -0.9926991079833414 1104 54-55 -1.0928433760792942 1104 56-57 -1.0592669404595938 1104 58-59 -1.1997392496415884 1104 60-61 -1.2289897778916767 1104 62-63 -1.2811561551143384 1104 64-65 -1.2049429008302255 1104 66-67 -1.2373261475382407 1104 68-69 -1.2305956348810483 1104 70-71 -1.2320294361362016 1104 72-73 -1.2406369327416336 1104 74-75 -1.0819512332881587 1104 76-77 -1.031710632721115 1104 78-79 -0.9113979867615924 1104 80-81 -0.9238881226121727 1104 82-83 -0.9516270372057711 1104 84-85 -0.9443543163778649 1104 86-87 -0.7856868803053914 1104 88-89 -0.9425609661528007 1104 90-91 -0.8531139017717777 1104 92-93 -1.0462737243492413 1104 94-95 -1.318969887719195 1104 96-97 -1.26808840587762 1104 98-99 -1.2981990819650697 1104 100 -1.3483152799103415 1105 1 -0.4635294412963553 1105 2 -0.448338734385878 1105 3 -0.4919559153439188 1105 4 -0.2968046965099589 1105 5 -0.38087275415664834 1105 6 -0.4256150020887688 1105 7 -0.45635289298144244 1105 8 -0.48722924161248926 1105 9 -0.6643288830232166 1105 10-11 -0.6198833274601228 1105 12-13 -0.611395712520796 1105 14-15 -0.8701971250372154 1105 16-17 -0.8894679426238596 1105 18-19 -0.8141493703195266 1105 20-21 -0.8904967988050458 1105 22-23 -0.8886785204964553 1105 24-25 -0.8793383025070725 1105 26-27 -1.0565841788966281 1105 28-29 -1.071420704862497 1105 30-31 -0.9263774693717721 1105 32-33 -0.8618128631267652 1105 34-35 -0.8728947705482497 1105 36-37 -0.9920856013944004 1105 38-39 -1.02540511730178 1105 40-41 -1.0393485792661608 1105 42-43 -0.8916504688848903 1105 44-45 -0.9592372312273838 1105 46-47 -1.1101419272732045 1105 48-49 -1.1054962920953741 1105 50-51 -1.1392537494762607 1105 52-53 -1.1319757817932299 1105 54-55 -1.1695459413044489 1105 56-57 -1.1347584153920565 1105 58-59 -1.1864783735016928 1105 60-61 -1.1032268795175284 1105 62-63 -0.9859194648961491 1105 64-65 -1.1188778410537026 1105 66-67 -1.1589639400601257 1105 68-69 -1.2392501974255694 1105 70-71 -1.2386401019157276 1105 72-73 -1.299084273281558 1105 74-75 -1.3016999289770652 1105 76-77 -1.1270138826495852 1105 78-79 -1.0749643942942413 1105 80-81 -0.9393048984914927 1105 82-83 -1.2026137615079122 1105 84-85 -0.888244066566525 1105 86-87 -0.9249258097429767 1105 88-89 -0.854947787042132 1105 90-91 -0.8553049355811382 1105 92-93 -1.072891157712725 1105 94-95 -1.0891655271820682 1105 96-97 -1.0234097923070244 1105 98-99 -0.9691025994285347 1105 100 -1.0125925297869678 1106 1 -0.4585047752098532 1106 2 -0.4354824832114126 1106 3 -0.4298333411312889 1106 4 -0.25334400306818594 1106 5 -0.29438164407329026 1106 6 -0.42311156306709563 1106 7 -0.46474799415435797 1106 8 -0.44291854196770686 1106 9 -0.6105308849534481 1106 10-11 -0.6033663312129462 1106 12-13 -0.6164970610369025 1106 14-15 -0.8214676930322398 1106 16-17 -0.8047585730229869 1106 18-19 -0.8468568210922598 1106 20-21 -0.8306514361935058 1106 22-23 -0.7211366478989731 1106 24-25 -0.8277149782319597 1106 26-27 -0.7355681532380629 1106 28-29 -0.8508989416478059 1106 30-31 -0.7288551613412508 1106 32-33 -0.8954845740719719 1106 34-35 -0.9690020568499946 1106 36-37 -0.8816440599738016 1106 38-39 -0.9162283228531081 1106 40-41 -1.0164157152178959 1106 42-43 -0.9482460928438385 1106 44-45 -1.02338359443916 1106 46-47 -0.9915410902773658 1106 48-49 -1.0794039124018369 1106 50-51 -1.0653014994256367 1106 52-53 -1.1134877905467704 1106 54-55 -1.156963637397098 1106 56-57 -1.2318991755982864 1106 58-59 -1.1425000023938523 1106 60-61 -1.2547045812932325 1106 62-63 -1.1342999946970522 1106 64-65 -1.3388590073928839 1106 66-67 -1.1994734432081557 1106 68-69 -1.2417965101922803 1106 70-71 -1.155428947353137 1106 72-73 -1.215088249821413 1106 74-75 -1.19346937860589 1106 76-77 -1.1626368621103254 1106 78-79 -0.9833188566301132 1106 80-81 -0.9504781556281436 1106 82-83 -0.985697155731085 1106 84-85 -0.8690202375670886 1106 86-87 -1.0225376965343997 1106 88-89 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1107 46-47 -1.1424374099430992 1107 48-49 -1.119187907245994 1107 50-51 -1.1268439025525012 1107 52-53 -1.0921138802243107 1107 54-55 -1.0360934941560913 1107 56-57 -1.130905444633541 1107 58-59 -1.0965539167370153 1107 60-61 -1.1950305475143423 1107 62-63 -1.342895834296165 1107 64-65 -1.099601062251054 1107 66-67 -1.0377910165110293 1107 68-69 -1.148498144960982 1107 70-71 -1.0363295801045425 1107 72-73 -1.0935487176714886 1107 74-75 -1.1738549622853256 1107 76-77 -1.1703014989013987 1107 78-79 -0.9363545506413438 1107 80-81 -0.9442025001334642 1107 82-83 -0.9700316015955721 1107 84-85 -0.8802602723145192 1107 86-87 -1.0175579252261358 1107 88-89 -0.9749541038903295 1107 90-91 -0.8904438517271913 1107 92-93 -0.9445938924241801 1107 94-95 -1.0561949185840689 1107 96-97 -1.1496586815775132 1107 98-99 -1.0918463875204232 1107 100 -1.2202679432781736 1108 1 -0.48272230348691636 1108 2 -0.4791526654542544 1108 3 -0.5320888300510767 1108 4 -0.30663732226820173 1108 5 -0.41165866022942055 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46-47 1.4350231772667428 2310 48-49 1.488199646169214 2310 50-51 1.4403325907193079 2310 52-53 1.5187609509420241 2310 54-55 1.4398039586041094 2310 56-57 1.4600528679614655 2310 58-59 1.4597835921624096 2310 60-61 1.4255582915029095 2310 62-63 1.4701603950994055 2310 64-65 1.5814555139043307 2310 66-67 1.721776515211765 2310 68-69 1.5617825361829105 2310 70-71 1.5796602074203747 2310 72-73 1.6624746148796419 2310 74-75 1.6290037385710647 2310 76-77 1.5526151148295888 2310 78-79 1.3526667646246082 2310 80-81 1.3313601998699767 2310 82-83 1.3091911332931012 2310 84-85 1.344867433445831 2310 86-87 1.4605055308232977 2310 88-89 1.485130777531264 2310 90-91 1.5089478488556765 2310 92-93 1.5197368038406296 2310 94-95 1.639789268969146 2310 96-97 1.644256630309524 2310 98-99 1.6605688493847843 2310 100 1.6171672198608604 2311 1 0.5317114160903209 2311 2 0.5794385407727667 2311 3 0.6039421783107422 2311 4 0.47107663976532876 2311 5 0.13945268972210556 2311 6 0.30611915799846656 2311 7 0.534107151726964 2311 8 0.27431956747311403 2311 9 0.4579942823537593 2311 10-11 0.6208137093502089 2311 12-13 0.6625844330468951 2311 14-15 0.866033465822305 2311 16-17 1.083905902098877 2311 18-19 1.0534581864413965 2311 20-21 1.100355418072759 2311 22-23 1.1051767021012182 2311 24-25 1.063839319131354 2311 26-27 1.0553383031021326 2311 28-29 1.0447910067607253 2311 30-31 1.079398055293673 2311 32-33 1.2559115741236226 2311 34-35 1.2049253837667422 2311 36-37 1.3057111302945188 2311 38-39 1.4132968173740466 2311 40-41 1.3705608398623639 2311 42-43 1.409947549773257 2311 44-45 1.4039257865966945 2311 46-47 1.5167560990017677 2311 48-49 1.5534773291887518 2311 50-51 1.5366347576518535 2311 52-53 1.5243110960906492 2311 54-55 1.4960990011867281 2311 56-57 1.5397596432251675 2311 58-59 1.5950040088647484 2311 60-61 1.5855146256446844 2311 62-63 1.6574358771238238 2311 64-65 1.6788949372433564 2311 66-67 1.6403436647454441 2311 68-69 1.64300828345376 2311 70-71 1.6349648714977292 2311 72-73 1.6667837157846677 2311 74-75 1.5445455750229726 2311 76-77 1.3770253872992804 2311 78-79 1.3373340094792994 2311 80-81 1.3880542822749362 2311 82-83 1.3617560032111875 2311 84-85 1.3938362674230298 2311 86-87 1.5060879046939917 2311 88-89 1.4585695643468917 2311 90-91 1.511309266264277 2311 92-93 1.554563009453691 2311 94-95 1.5818114144902147 2311 96-97 1.6808093521053884 2311 98-99 1.7472965647187166 2311 100 1.6628975583339418 2312 1 0.2749087807243278 2312 2 0.251316180213248 2312 3 0.4723884088987056 2312 4 0.346207357861573 2312 5 0.47084916396215704 2312 6 0.5561360937631861 2312 7 0.6012177975672444 2312 8 0.4949199777954263 2312 9 0.6287440370173769 2312 10-11 0.5269864766900767 2312 12-13 0.6546061552859896 2312 14-15 1.0281099726285632 2312 16-17 1.0545973847281758 2312 18-19 1.0597335219738753 2312 20-21 1.1328049005752305 2312 22-23 0.8141998453345565 2312 24-25 0.7621720163156667 2312 26-27 1.0048429118593418 2312 28-29 1.2438570504224202 2312 30-31 1.1238920400320396 2312 32-33 1.02413654684063 2312 34-35 1.1972171494059722 2312 36-37 1.2708456541388387 2312 38-39 1.2669370098386423 2312 40-41 1.3016789978753422 2312 42-43 1.2663249581194904 2312 44-45 1.2253045083161354 2312 46-47 1.3750569860519164 2312 48-49 1.4573998071035206 2312 50-51 1.4961473971195787 2312 52-53 1.4709762706349139 2312 54-55 1.4937346507795084 2312 56-57 1.5766036935021646 2312 58-59 1.5940124925992265 2312 60-61 1.5884800718547112 2312 62-63 1.6221268820840535 2312 64-65 1.7195929731157165 2312 66-67 1.68850851518809 2312 68-69 1.6893048434934244 2312 70-71 1.4813886169631587 2312 72-73 1.6548632904721856 2312 74-75 1.7115852516495664 2312 76-77 1.4637505280735716 2312 78-79 1.4702577181628413 2312 80-81 1.28436597613873 2312 82-83 1.2907932449268422 2312 84-85 1.216268427765275 2312 86-87 1.3656099161104933 2312 88-89 1.0124497400032801 2312 90-91 1.4280220547667284 2312 92-93 1.5188881306852835 2312 94-95 1.6446558111608738 2312 96-97 1.7414769921502327 2312 98-99 1.7666216345547525 2312 100 1.7813393924345355 2313 1 -0.24902795365290586 2313 2 0.1409334082254894 2313 3 0.3781437162872301 2313 4 0.25272737890445285 2313 5 0.3592745174850194 2313 6 0.46212383046532324 2313 7 0.2826675140459045 2313 8 0.24567044871479027 2313 9 0.43456170940295635 2313 10-11 0.5602427174322955 2313 12-13 0.725018282459672 2313 14-15 0.777465655540432 2313 16-17 0.9353714652535672 2313 18-19 0.8247513041800332 2313 20-21 1.0338499536794217 2313 22-23 1.026111514316426 2313 24-25 0.8938729200929671 2313 26-27 1.1017127223521754 2313 28-29 1.0333906705498848 2313 30-31 0.991243009507734 2313 32-33 1.134263540520493 2313 34-35 1.1768734176639484 2313 36-37 1.0161368544317 2313 38-39 0.7898833286674147 2313 40-41 0.9358541374541431 2313 42-43 1.0188045273706976 2313 44-45 1.2594507836288074 2313 46-47 1.235667687255095 2313 48-49 1.2678145494857844 2313 50-51 1.2107628220621862 2313 52-53 1.1676152427579467 2313 54-55 1.2576784398561145 2313 56-57 1.3746029225762513 2313 58-59 1.2699504979132854 2313 60-61 1.265593011894616 2313 62-63 1.3409746872482842 2313 64-65 1.4382248341745054 2313 66-67 1.4911926040530616 2313 68-69 1.4837827230107976 2313 70-71 1.4240932911257431 2313 72-73 1.5055231836577363 2313 74-75 1.06778541722894 2313 76-77 1.1650627082181053 2313 78-79 1.0516291299027358 2313 80-81 1.1132174541103694 2313 82-83 1.1187370212759014 2313 84-85 1.18805566069884 2313 86-87 1.2717958086511771 2313 88-89 1.2129093106363378 2313 90-91 1.3604781265355683 2313 92-93 1.104888749486662 2313 94-95 1.167135831937081 2313 96-97 1.4910416644703552 2313 98-99 1.4939413518334312 2313 100 1.5876517422797427 2314 1 -0.15821123738772158 2314 2 0.03257545452877153 2314 3 0.2699284309996024 2314 4 -0.007679767217517508 2314 5 0.35905465607463327 2314 6 0.3236176715729684 2314 7 0.2542308825230748 2314 8 0.35082556371268225 2314 9 0.4645983738423851 2314 10-11 0.6058435661080708 2314 12-13 0.6238012627893212 2314 14-15 0.7174812372563366 2314 16-17 0.9406759572064445 2314 18-19 1.0461249102560117 2314 20-21 0.6137675637912565 2314 22-23 0.7859639084175569 2314 24-25 1.0745973446562616 2314 26-27 1.0805715450292155 2314 28-29 1.088982243542894 2314 30-31 0.985581667637554 2314 32-33 1.0382476117491493 2314 34-35 1.120097339086307 2314 36-37 1.07704758873124 2314 38-39 1.0784650257864072 2314 40-41 1.1340874642578243 2314 42-43 0.988240302883078 2314 44-45 1.0274650182686926 2314 46-47 1.144943414752177 2314 48-49 1.0723832481262718 2314 50-51 1.1896670839566568 2314 52-53 1.2405795792335539 2314 54-55 1.3627076578096151 2314 56-57 1.2106433777913423 2314 58-59 1.2452288354819459 2314 60-61 1.3357909676665187 2314 62-63 1.08784805763068 2314 64-65 1.151035920627983 2314 66-67 1.2776637926374335 2314 68-69 1.4080251716479424 2314 70-71 1.41969029356855 2314 72-73 1.4462065787544205 2314 74-75 1.4437324626511625 2314 76-77 1.2074327211969056 2314 78-79 1.1605632135198434 2314 80-81 1.1114752000210615 2314 82-83 1.0665687745230663 2314 84-85 1.0414242004809644 2314 86-87 1.2118016853373703 2314 88-89 1.359945788130954 2314 90-91 1.3308573472172114 2314 92-93 1.4338271802414972 2314 94-95 1.4372878118078702 2314 96-97 1.5257198823585618 2314 98-99 1.5631216211949663 2314 100 1.5269471839569881 2315 1 0.03647373792186315 2315 2 0.2262766053085663 2315 3 0.2624970817552956 2315 4 0.04297881044215046 2315 5 0.3342875738181945 2315 6 0.48676616208070556 2315 7 0.533810647677079 2315 8 0.5021533408229644 2315 9 0.7996745860058425 2315 10-11 0.7108379434507626 2315 12-13 0.7647478439066475 2315 14-15 0.8941563759178877 2315 16-17 0.8816934567475201 2315 18-19 0.9592804849156025 2315 20-21 1.0072100143599982 2315 22-23 1.0775963744554815 2315 24-25 1.0447172647056178 2315 26-27 1.1369965779313276 2315 28-29 1.1452420337963929 2315 30-31 1.0982957739260968 2315 32-33 1.1621852773652321 2315 34-35 1.1751807608162466 2315 36-37 1.2169350078040466 2315 38-39 1.2354830776251262 2315 40-41 1.1153209100443036 2315 42-43 1.2680985535891764 2315 44-45 1.1682343181160846 2315 46-47 1.124029268106149 2315 48-49 1.1706853647525293 2315 50-51 1.125725011672749 2315 52-53 1.1847514489805278 2315 54-55 1.1957346866145784 2315 56-57 1.300962498275105 2315 58-59 1.3784884906780874 2315 60-61 1.3675597653229659 2315 62-63 1.3417245528525896 2315 64-65 1.4350794113454413 2315 66-67 1.4335860933049318 2315 68-69 1.3338299061567014 2315 70-71 0.8598901873328941 2315 72-73 1.1678888574719863 2315 74-75 1.4233600243854738 2315 76-77 1.2539954463479681 2315 78-79 1.0928620595455243 2315 80-81 1.1202029342655635 2315 82-83 1.0983806686690976 2315 84-85 1.0520704827742726 2315 86-87 1.0297065789274953 2315 88-89 1.1282485596345495 2315 90-91 1.1772236121252106 2315 92-93 1.1525363927565309 2315 94-95 1.3200042500986342 2315 96-97 1.4333618128233638 2315 98-99 1.4049800287876657 2315 100 1.3804464798899048 2316 1 0.6268265004334417 2316 2 0.6308359183002459 2316 3 0.6058314566007077 2316 4 0.4480616390282677 2316 5 0.4907680699808594 2316 6 0.5000676589415534 2316 7 0.6338538751577403 2316 8 0.6278644829028295 2316 9 0.6770553920858831 2316 10-11 0.7001322309144768 2316 12-13 0.7962815114501964 2316 14-15 0.9939231751123572 2316 16-17 1.025288173534392 2316 18-19 1.023195870800805 2316 20-21 1.0596402357076968 2316 22-23 1.029052223757887 2316 24-25 1.1273844608165362 2316 26-27 1.2266306034962398 2316 28-29 1.1868407830223902 2316 30-31 1.0618184880116033 2316 32-33 1.0542996862338967 2316 34-35 0.9555462230274756 2316 36-37 0.9863247941540436 2316 38-39 0.9403115085237417 2316 40-41 0.9814947058128993 2316 42-43 1.1409183733934398 2316 44-45 1.1212194525374102 2316 46-47 1.124616204677828 2316 48-49 0.9048669429303047 2316 50-51 1.0021668772054824 2316 52-53 1.1828773854308992 2316 54-55 1.2652531754237444 2316 56-57 1.2500554627503249 2316 58-59 1.2138584428195287 2316 60-61 1.1934606993577503 2316 62-63 1.2821509149987165 2316 64-65 1.3139291824082875 2316 66-67 1.2621650278342642 2316 68-69 1.2572857742981753 2316 70-71 1.1373767818262195 2316 72-73 1.221106341430179 2316 74-75 1.271524646754365 2316 76-77 0.9910447028876597 2316 78-79 1.0077171304724288 2316 80-81 1.0395797336493047 2316 82-83 0.9493367355130466 2316 84-85 0.9466439756784517 2316 86-87 0.8872996451892803 2316 88-89 0.8988290543550299 2316 90-91 1.0010630572408026 2316 92-93 1.0580538510333213 2316 94-95 0.9299323448104921 2316 96-97 1.1071550802712338 2316 98-99 1.2187362113456466 2316 100 0.9075544572922603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 2.0 12 34.0 13 120.0 14 497.0 15 1476.0 16 3177.0 17 6216.0 18 10430.0 19 14558.0 20 19702.0 21 26727.0 22 32433.0 23 36147.0 24 40381.0 25 48012.0 26 58965.0 27 73373.0 28 90566.0 29 109864.0 30 134868.0 31 166942.0 32 209161.0 33 269087.0 34 364932.0 35 478748.0 36 680511.0 37 945321.0 38 751325.0 39 84746.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.16999864906572 21.344224712990897 14.83345171011313 23.652324927830257 2 14.965310552079467 22.142856682852 40.09739106242512 22.794441702643415 3 16.957503896780242 28.575479683397475 32.01366239811538 22.453354021706907 4 12.125427445713026 18.905386862658503 40.08687719385634 28.88230849777214 5 12.510342079716558 38.453644941751605 34.67727773706065 14.358735241471193 6 27.656223803665387 38.707043914636415 20.293718804559486 13.34301347713871 7 25.548459059524415 33.60846089709518 23.954151750715493 16.888928292664914 8 25.90701749151035 34.93441944805388 21.494522592601577 17.664040467834198 9 26.973781801890105 16.023882773219917 21.035139139072097 35.967196285817884 10-11 24.19004856703212 26.34220523177534 29.716072630725687 19.75167357046685 12-13 25.047179036042834 24.380983784064593 28.966908449163743 21.60492873072883 14-15 22.7980018167449 25.824697104531957 26.82759910925189 24.549701969471258 16-17 22.05988693154637 28.07627242260824 27.467126888968863 22.396713756876526 18-19 21.824201919442 27.61099303119807 28.9690217509139 21.59578329844603 20-21 22.897863441248926 27.02899879184223 28.29631936925062 21.776818397658218 22-23 22.520738641913322 27.024377604606624 28.17088215891822 22.28400159456183 24-25 22.150746925320135 27.001476172633094 28.508571901456968 22.339205000589804 26-27 22.1952538156634 27.066547324938124 28.295517397162484 22.442681462235996 28-29 22.138312178778698 27.627015074505255 28.27112406243872 21.963548684277328 30-31 23.026748732449455 26.886454498217212 28.385157188933164 21.70163958040017 32-33 22.952171918218056 27.116789112573304 28.01618093725598 21.91485803195266 34-35 22.985823025781755 27.130977343015182 28.2784541404896 21.604745490713462 36-37 22.605219225307177 27.30399721448917 28.11470927733795 21.976074282865703 38-39 22.617026265208004 27.198343459119823 28.216010766485105 21.968619509187068 40-41 22.671285492762262 26.64997827009335 28.596202949707365 22.082533287437023 42-43 22.476976627838468 26.873729875916 28.107281792507948 22.54201170373759 44-45 22.023201944813025 27.24018842281499 28.347901862383672 22.38870776998831 46-47 22.133262741134995 26.602792320779713 27.957827602386864 23.306117335698428 48-49 22.236071487685187 26.659419774933458 28.257489283695524 22.847019453685835 50-51 22.31264851189684 27.026170297363006 27.907043897261325 22.75413729347883 52-53 21.975516452349297 27.253214760550858 27.803098901960066 22.968169885139776 54-55 21.940127124206896 26.931662446404058 28.108097547696264 23.02011288169278 56-57 22.412244112862332 26.90013065115626 27.721498362245033 22.96612687373637 58-59 21.96016513257053 27.036556497063252 28.215936845239685 22.787341525126532 60-61 22.408647259428516 27.381607131586843 27.673743984344373 22.53600162464026 62-63 22.366135230881273 27.180850064696454 27.62649618932259 22.826518515099686 64-65 21.8776153973987 26.85899602234153 27.765144667305346 23.498243912954422 66-67 21.896892156685883 26.83744857557897 27.650510657708573 23.61514861002657 68-69 21.565435010766414 27.014430143515337 27.859344895475402 23.560789950242846 70-71 21.778407666854445 26.615088831040083 28.079695476933008 23.526808025172464 72-73 21.834099490311974 27.008740760911348 27.6156354435686 23.54152430520808 74-75 22.024262175187072 27.053019974192004 27.432280861556197 23.490436989064726 76-77 21.73528122140106 26.8709478346723 27.652027730692936 23.741743213233708 78-79 21.490591898224782 26.828172306460957 27.893526420447106 23.78770937486716 80-81 21.591336729894394 27.149593591228964 27.499532548874132 23.759537130002506 82-83 22.003508175973955 26.975109729809464 27.627970155810853 23.393411938405723 84-85 22.09726988845029 27.190038271387518 27.077532848533792 23.6351589916284 86-87 21.62662221841368 27.281973007608563 27.397378248920095 23.69402652505766 88-89 21.67625187767287 26.84393194444698 27.46353848452106 24.016277693359093 90-91 22.16126240296049 26.64924626913988 27.548275355949787 23.641215971949848 92-93 21.960529005460803 27.00938677211504 27.303533974978915 23.726550247445243 94-95 21.517367969085445 26.850933877200518 27.38620652640618 24.245491627307857 96-97 21.444287301053677 26.795352505685717 27.536942650820084 24.223417542440522 98-99 21.426415074893722 26.96469202438391 27.250649674395195 24.35824322632717 100 21.396598434096216 26.926923488170267 27.405849745206766 24.27062833252675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 98.0 1 91.0 2 243.5 3 403.5 4 301.0 5 234.5 6 363.0 7 626.0 8 971.5 9 1217.5 10 1252.5 11 1259.0 12 1462.5 13 1745.5 14 2086.5 15 2435.0 16 2782.5 17 3210.5 18 3633.0 19 4050.0 20 4623.0 21 5307.5 22 6273.0 23 7723.0 24 9534.0 25 12027.0 26 15516.0 27 20033.5 28 26202.5 29 34786.0 30 45431.5 31 57066.5 32 71240.5 33 85883.0 34 99637.0 35 112662.5 36 127624.0 37 146800.0 38 157290.0 39 156589.5 40 156268.5 41 157959.0 42 160411.0 43 164890.5 44 170946.0 45 180003.0 46 187146.0 47 191579.5 48 195499.5 49 196219.0 50 194432.5 51 190249.0 52 181658.0 53 169842.5 54 157638.0 55 142503.5 56 126165.5 57 109167.0 58 91364.0 59 74478.0 60 57681.5 61 43660.5 62 33861.0 63 25678.5 64 18320.0 65 12924.5 66 9342.5 67 7049.0 68 5410.5 69 4014.5 70 2990.0 71 2292.5 72 1720.0 73 1298.0 74 996.0 75 701.0 76 482.0 77 294.0 78 180.5 79 130.5 80 91.5 81 53.0 82 25.0 83 15.5 84 13.0 85 8.5 86 4.0 87 1.0 88 1.0 89 2.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04928801783814081 2 0.0 3 0.0 4 0.015542040468124541 5 0.0038425763035832773 6 0.0 7 1.9320216051536032E-4 8 3.434705075828628E-4 9 0.0017173525379143141 10-11 6.225402949939389E-4 12-13 3.005366941350049E-4 14-15 3.2200360085893384E-5 16-17 9.660108025768016E-5 18-19 0.0 20-21 2.1466906723928925E-5 22-23 0.0 24-25 5.3667266809822316E-5 26-27 3.2200360085893384E-5 28-29 8.050090021473346E-4 30-31 8.050090021473346E-4 32-33 0.013524151236075222 34-35 0.02205724665883697 36-37 0.029570664012212096 38-39 0.019685153465842823 40-41 1.39534893705538E-4 42-43 0.0016636852711044915 44-45 0.00868336376982925 46-47 0.012762076047375745 48-49 0.0052379252406386575 50-51 0.009810376372835519 52-53 0.015359571760971144 54-55 0.009606440758958194 56-57 0.0027262971539389735 58-59 0.008125224195007098 60-61 0.0015241503773989536 62-63 8.694097223191215E-4 64-65 0.017581396606897788 66-67 0.042611809846998916 68-69 0.05193918081854603 70-71 0.045810378948864326 72-73 0.03776028892739098 74-75 0.026801433044825265 76-77 0.0269409679385308 78-79 0.01157066272419769 80-81 0.0019749554186014612 82-83 0.026017890949401858 84-85 0.04535957390766182 86-87 0.04576744513541647 88-89 0.056597499577638606 90-91 0.03287656764769715 92-93 0.024493740572002907 94-95 0.007148479939068332 96-97 0.0035849734228961304 98-99 0.021466906723928925 100 0.017838999487584935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4658333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.70562975946183 #Duplication Level Percentage of deduplicated Percentage of total 1 81.05266602829757 55.68774463157522 2 12.274539449254016 16.8665992573671 3 3.29678830332122 6.795237496899362 4 1.2744209119245886 3.50239565329626 5 0.622409234431703 2.1381509209867335 6 0.3725654411621311 1.5358405956993564 7 0.23295350606314177 1.1203652137119935 8 0.16831884482478776 0.925156178725774 9 0.11330966413908188 0.7006510649257858 >10 0.550959836328464 6.44426512782124 >50 0.026683842052475645 1.2447442419902497 >100 0.013168599473308234 1.7639507140647894 >500 8.22817472364762E-4 0.37099863356334145 >1k 2.861972765324257E-4 0.40452455835045914 >5k 1.073239786996195E-4 0.4993757110222849 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8560 0.1837567215568316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6590 0.1414669153106916 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 5167 0.11091950704254076 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.293381344785785E-5 0.0 3 0.0 0.0 0.0 4.293381344785785E-5 0.0 4 0.0 0.0 0.0 8.58676268957157E-5 0.0 5 0.0 0.0 0.0 1.0733453361964462E-4 0.0 6 0.0 0.0 0.0 1.0733453361964462E-4 0.0 7 0.0 0.0 0.0 1.0733453361964462E-4 0.0 8 0.0 0.0 0.0 1.0733453361964462E-4 0.0 9 0.0 0.0 0.0 3.0053669413500497E-4 2.1466906723928925E-5 10-11 0.0 0.0 0.0 4.7227194792643637E-4 2.1466906723928925E-5 12-13 0.0 0.0 0.0 5.152057613742941E-4 6.440072017178678E-5 14-15 0.0 0.0 0.0 5.474061214601876E-4 9.660108025768016E-5 16-17 0.0 0.0 0.0 6.332737483559032E-4 1.2880144034357354E-4 18-19 0.0 0.0 0.0 7.513417353375124E-4 1.2880144034357354E-4 20-21 0.0 0.0 0.0 8.801431756810859E-4 2.4686942732518266E-4 22-23 0.0 0.0 0.0 0.0016100180042946694 3.0053669413500497E-4 24-25 0.0 0.0 0.0 0.0027370306073009375 3.0053669413500497E-4 26-27 0.0 0.0 0.0 0.004529517318749003 3.0053669413500497E-4 28-29 0.0 0.0 0.0 0.008050090021473347 3.0053669413500497E-4 30-31 0.0 0.0 0.0 0.020404294841094444 3.112701474969694E-4 32-33 0.0 0.0 0.0 0.051992848085355856 3.2200360085893386E-4 34-35 0.0 1.0733453361964463E-5 0.0 0.10187120585840471 3.756708676687562E-4 36-37 0.0 3.220036008589339E-5 0.0 0.17949554057213168 3.8640432103072064E-4 38-39 0.0 6.440072017178678E-5 0.0 0.3232379479955598 3.8640432103072064E-4 40-41 0.0 8.58676268957157E-5 0.0 0.5132522728624167 3.8640432103072064E-4 42-43 0.0 1.0733453361964462E-4 0.0 0.7046190128528811 3.971377743926851E-4 44-45 0.0 1.0733453361964462E-4 0.0 0.9295599949595703 4.18604681116614E-4 46-47 0.0 1.0733453361964462E-4 0.0 1.1808945388833303 4.4007158784054295E-4 48-49 0.0 1.0733453361964462E-4 0.0 1.4607586018431915 4.7227194792643637E-4 50-51 0.0 1.0733453361964462E-4 0.0 1.7766334008324436 4.7227194792643637E-4 52-53 0.0 1.0733453361964462E-4 0.0 2.119910706254791 4.8300540128840084E-4 54-55 0.0 1.0733453361964462E-4 0.0 2.4945726293075223 4.937388546503653E-4 56-57 0.0 1.0733453361964462E-4 0.0 2.8866442137133603 5.044723080123298E-4 58-59 0.0 1.0733453361964462E-4 0.0 3.33931687580085 5.152057613742941E-4 60-61 0.0 1.0733453361964462E-4 0.0 3.8084117215321447 5.152057613742941E-4 62-63 0.0 1.0733453361964462E-4 0.0 4.306132687379799 5.152057613742941E-4 64-65 0.0 1.0733453361964462E-4 0.0 4.767703382304356 5.152057613742941E-4 66-67 0.0 1.0733453361964462E-4 0.0 5.275223991071484 5.152057613742941E-4 68-69 0.0 1.0733453361964462E-4 0.0 5.836218664487919 5.152057613742941E-4 70-71 0.0 1.0733453361964462E-4 0.0 6.432011193703842 5.152057613742941E-4 72-73 0.0 1.0733453361964462E-4 0.0 7.009191914790119 5.259392147362586E-4 74-75 0.0 1.0733453361964462E-4 0.0 7.5722044774386035 5.366726680982231E-4 76-77 0.0 1.5026834706750248E-4 0.0 8.16047285584779 5.366726680982231E-4 78-79 0.0 1.5026834706750248E-4 0.0 8.812948752268248 5.366726680982231E-4 80-81 0.0 1.5026834706750248E-4 0.0 9.445964897743464 5.366726680982231E-4 82-83 0.0 1.5026834706750248E-4 0.0 10.108455106150632 5.58139574822152E-4 84-85 0.0 1.5026834706750248E-4 0.0 10.763002559069951 5.79606481546081E-4 86-87 0.0 1.5026834706750248E-4 0.0 11.422455200175685 6.118068416319743E-4 88 0.0 1.5026834706750248E-4 0.0 11.909560780648356 6.440072017178677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4505 0.0 44.85447 2 GGTATCA 6510 0.0 44.63136 1 GTATCAA 11960 0.0 40.33191 1 ACCTGGG 5535 0.0 35.488754 3 CAACGCA 14470 0.0 32.4073 5 ATCAACG 14475 0.0 32.302532 3 GTACCTG 6480 0.0 31.488163 1 TCAACGC 15110 0.0 31.038649 4 TATCAAC 15245 0.0 30.948412 2 AACGCAG 15765 0.0 30.28042 6 TAACGCA 680 0.0 29.025326 4 CCTGGGG 5155 0.0 27.712881 4 ATAACGC 730 0.0 27.68074 3 ACGCAGA 17505 0.0 26.921606 7 CGCAGAG 17895 0.0 26.33488 8 GTACATG 16465 0.0 25.727386 1 TACATGG 16600 0.0 24.940199 2 TGGGGGG 9480 0.0 24.037848 6 ACATGGG 16730 0.0 23.594753 3 ATGGGGG 9540 0.0 23.198149 5 >>END_MODULE